Detection and drug resistance analysis of virulence and adhesion factor genes of Escherichia coli from Tibetan piglets with diarrhea under house feeding conditions

doi:10.14088/j.cnki.issn0439-8114.2024.06.025

Abstract

Abstract: To effectively prevent and treat diarrhea in Tibetan piglets, PCR and Kirby Bauer paper diffusion methods were used,the virulence genes, adhesion factor genes and drug resistance of 102 strains of Escherichia coli from Tibetan piglets with diarrhea in Linzhi, Xizang were detected. The detection results of virulence genes and pili adhesion factor genes in Escherichia coli showed that the pili adhesion factor gene with the highest detection rate was F18 （45.10%）, followed by F4 （15.69%）, and no F41 gene was detected in all strains; among the 102 strains, the pili adhesion factor gene was not detected in 37 strains. The gene with the highest detection rate among virulence genes was Stx2e （25.49%）, followed by the STb：EAST-1 gene （21.57%） and the STa：STb gene （17.65%）. The detection results of Escherichia coli pili adhesion factor genes and non pili adhesion factor genes showed that 44.73% of the strains containing AIDA-1 gene had pili adhesion factor genes （F18, F4）, and 54.17% of the strains containing paa gene had pili adhesion factor genes （F18, F4）;no F4 gene was detected in the strains that the eae gene was detected. Escherichia coli exhibited high drug resistance to chloramphenicol, tetracycline, ampicillin, and streptomycin, with 86.27% of the total bacterial strains exhibiting resistance to three or more antibiotics.

Key words: Tibetan piglets, diarrhea source, Escherichia coli, virulence genes, drug resistance, pili adhesion factor genes, non pili adhesion factor genes

CLC Number:

S828

LI Long, TAN Zhan-kun, LI Wen-feng, LIU Suo-zhu. Detection and drug resistance analysis of virulence and adhesion factor genes of Escherichia coli from Tibetan piglets with diarrhea under house feeding conditions[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(6): 156-160.

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