HUBEI AGRICULTURAL SCIENCES ›› 2018, Vol. 57 ›› Issue (6): 108-110.doi: 10.14088/j.cnki.issn0439-8114.2018.06.027

• Biological Engineering • Previous Articles     Next Articles

Effects of Developmental Potency of Reconstructed Embryos on Pig Handmade Clone of Amino Acid

LYU Ling-yan, CHEN Bao-jian, WU Yong-shao, SUN Ru-yu, WANG Yan-ling, MA Qing-yan, PAN Tian-biao   

  1. Guangxi Key Laboratory of Livestock Genetic Improvement,Guangxi Institute of Animal Sciences,Nanning 530001,China
  • Received:2017-12-15 Online:2018-03-25 Published:2020-01-06

Abstract: Effect of developmental potency of reconstructed embryos on pig handmade clone(HMC) of amino acid was investigated. In this study,we compared with the effect of the essential amino acid(EAA) and the essential amino acid (NEAA) on oocyte maturation rate and porcine HMC reconstructed embryos developmental potency. The results showed that the difference of maturation rate was not significant between the group of EAA,NEAA and EAA+NEAA in maturation medium,yet was significantly higher than the control group(P<0.05);the cleavage rate of EAA group, NEAA group and the control group was significantly higher than EAA+NEAA group in embryo culture medium(P<0.05),as for the blastocyst rate and the total number of cells,the group of NEAA was significantly higher than the group of control, EAA+NEAA,EAA(P<0.05).

Key words: amino acid, porcine, reconstructed embryos, developmental potency

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