HUBEI AGRICULTURAL SCIENCES ›› 2020, Vol. 59 ›› Issue (14): 156-158.doi: 10.14088/j.cnki.issn0439-8114.2020.14.032

• Detection Analysis • Previous Articles     Next Articles

Extraction separation and determination of kaempferol in Camellia euphlebia Merr.ex Sealy

CHEN Qiu-hong1, LIU Peng2, WEI Ying-liang1, LIU Bu-ming3, HUANG Yan3, MO Jian-guang1   

  1. 1. Guangxi Research Center of Analysis and Testing,Nanning 530022, China;
    2. Nanyang Second General Hospital,Nanyang 473012,Henan,China;
    3. Guangxi Institute of Traditional Medical and Pharmaceutical Science, Nanning 530022, China
  • Received:2019-11-10 Online:2020-07-25 Published:2020-09-14

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Abstract: Solvent extraction method was used to extract and separate the ethyl acetate part of dried leaves of Camellia euphlebia Merr.ex Sealy, and determine its content. The results showed that the compound kaempferol was first isolated from the ethyl acetate part of Camellia euphlebia Merr.ex Sealy; the content determination method of kaempferol in Camellia euphlebia Merr.ex Sealy was established by HPLC. The kaempferol ranged from 0.10 to 0.40 μg/mL. The internal linear relationship was good, the average recovery rate was 99.84% （RSD=1.59%）, and the measured content of kaempferol was 0.318 2~0.364 0 mg/g.

Key words: Camellia euphlebia Merr. ex Sealy, kaempferol, content determination

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