Identification and screening of energy metabolism-related membrane protein genes and their upstream transcription factors in chicken liver

doi:10.14088/j.cnki.issn0439-8114.2022.24.028

Abstract

Abstract: Tran membrane proteins（TMEMs） participates in the physiological and biochemical processes of cells and plays an important role in the maintenance of cell function. In order to explore the expression pattern of TMEMs genes in liver tissues, the eight TMEMs genes were chosen to detect their expression levels in liver tissues of broilers and laying hens under the different feeding conditions. The results showed that TMEMs genes maintained a high expression level in the liver tissue of broilers. The expression of TMEM86A gene was high in the liver of broilers and low in the liver of laying hens, and feeding restriction would promote the up-regulated expression of TMEM86A gene. Through the prediction of transcription factors using the promoter region of TMEM86A gene and detection of the promoter activity, it was found that SP1 and CREB1 may be the upstream transcription factors of TMEM86A.

Key words: TMEM86A gene, TMEMs, liver, chicken

CLC Number:

S831

SHENG Jie, LI Gang, HUANG Tao, PI Jin-song, GONG Yan-zhang, PAN Ai-luan, WU Yan, ZHANG Hao, FU Ming, LIANG Zhen-hua. Identification and screening of energy metabolism-related membrane protein genes and their upstream transcription factors in chicken liver[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(24): 132-135.

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