Isolation,Screening and Identification of Antagonistic Bacteria on Leaf Spot Disease of Thunbergia grandiflora

doi:10.14088/j.cnki.issn0439-8114.2018.24.024

Abstract

Abstract: Using the plate confrontation assay,2 strains（bjq-a, bjq-d） with antagonistic effects on leaf spot disease of Thunbergia grandiflora were screened from 6 bacteria isolated from health leaves of T. grandiflora conventional tissue separation,strain bjq-a was the biggest antagonism width. Antibacterial spectrum tests showed it had significantly antagonistic activities to 9 plant pathogens. Based on morphological and 16S rDNA sequence analysis, strains bjq-a and bjq-d were identified as Bacillus methylotrophicus and B. amyloliquefaciens,respectively. When observed under the microscope,the mycelia of Colletotrichum gloeosporioides showed swollen, deformity and breakage.

Key words: Thunbergia grandiflora, leaf spot disease, antagonistic bacteria, screening, identification

CLC Number:

S435.67
S476

SONG Li-sha, JIANG Ni. Isolation,Screening and Identification of Antagonistic Bacteria on Leaf Spot Disease of Thunbergia grandiflora[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(24): 89-92.

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