关键词: 金沙鲈鲤(Percocypris pingi), 全长转录组, 测序, 抗菌肽基因, 鉴定

Abstract: In order to further enrich the Percocypris pingi gene information database, the PacBio sequencing platform was used to perform full-length transcriptome sequencing of Percocypris pingi. A total of 499 007 full-length non-chimeric reads (FLNC) were obtained, and 70 321 isoforms were obtained through clustering and redundancy removal. The average length and N50 of the isoforms were 1 882 bp and 2 243 bp, respectively. By comparing isoforms with five public databases including NR, GO, KEGG, KOG, and Swiss-Prot, 57 521 isoforms were successfully annotated and 2 310 TFs, 16 225 LncRNAs, and 35 314 CDS were identified. Five antimicrobial peptide genes were identified, including two hepcidin genes and three hemoglobin genes. The hepcidin-1and hepcidin-2 of Percocypris pingi was closely related to hepcidin of Onychostoma macrolepis; the hemoglobin-1α of Percocypris pingi was closely related to hemoglobin α of Danio rerio; the hemoglobin-1β of Percocypris pingi was closely related to hemoglobin 1β and hemoglobin 2β of Ictalurus punctatus; the hemoglobin-2β of Percocypris pingi was closely related to hemoglobin 2β of Onychostoma macrolepis. Through annotation analysis of the full-length transcriptome of Percocypris pingi, the biological processes, metabolic pathways, or signaling pathways involved in the Percocypris pingi gene were elucidated, which could more effectively explore the role of antimicrobial peptides in fish immunity.

Key words: Percocypris pingi, full-length transcriptome, sequencing, antimicrobial peptide gene, identification