Screening and optimization of cordycepic acid extraction method of Cordyceps militaris CNZ

doi:10.14088/j.cnki.issn0439-8114.2020.09.030

Abstract

Abstract: In order to obtain the optimal cordycepic acid extraction method of Cordyceps militaris CNZ, six extraction methods were used to extract cordycepic acid of C. militaris CNZ, respectively, and the extraction yield of cordycepic acid with ultrasonic-associated ethanol extraction method was the maximum for 113.31 mg/g, so was selected as one suitably initial method. Subsequently, the extraction conditions, including extraction times, solid-liquid ratio, ultrasonic time and ethanol concentration, were further optimized by single factor experiments and orthogonal experiments. One optimized cordycepic acid extraction method of C. militaris CNZ was obtained as that： ethanol concentration was 20%（volume fraction）, solid-liquid ratio was 1:10（g:mL）, ultrasonic time was 20 min and extraction times was three. Using the optimized extraction method, the extraction yield of cordycepic acid of C. militaris CNZ was 161.06 mg/g, which was about 42.1% higher than that using the initial extraction method.

Key words: Cordyceps militaris, cordycepic acid, extraction method, optimization

CLC Number:

S567.3⁺9

LIN Ai-hua, MIAO Li-yun, WANG Jun-ling, LI Hui, SONG Fa-jun, ZHANG Peng. Screening and optimization of cordycepic acid extraction method of Cordyceps militaris CNZ[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(9): 141-144.

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