Analysis of HMW-GS composition of two Aegilops diploid species based on relative mobility

doi:10.14088/j.cnki.issn0439-8114.2025.08.027

Abstract

Abstract: Aegilops is considered as an important gene source for improving modern common wheat, which has many excellent genes or traits. In order to understand the genetic diversity of high molecular weight glutenin subunits (HMW-GS) in Ae. diploid species, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was used to analyze HMW-GS in 40 Ae. accession (Ae. comosa and Ae. markgraﬁi). The results showed the total of 17 relative mobilities were identified among the 40 accessions, of which five relative mobilities (0.82~0.98) were lower than that of Chinese Spring 1Dx2 (1.00), and one relative mobility (1.49) was higher than that of Chinese Spring 1Dy12 (1.40). Analysis of genetic similarity coefficients (GS) among the accessions revealed a range of 0.00~1.00, with an average value of 0.23. And, it was found that the average GS (0.58) of Ae. markgraﬁi accessions was significantly higher than that of Ae. comosa accessions(0.31). The clustering results showed that 40 Ae. accessions were divided into two groups at the GS 0.10, of which 19 Ae. comosa accessions were clustered in Group1. And, the Group2 including 21 Ae. accessions could be further divided into two sub-groups, which indicated that Ae. accessions clustering together had the same species.

Key words: Aegilops comosa, Ae. markgraﬁi, HMW-GS, genetic diversity, clustering analysis

CLC Number:

S545

ZHANG Hai-hui, ZHANG Yan-ru, SONG Lin-xuan, HAO Zhi-hua, ZHANG Yi-lin, WEI Sa, HU Xi-gui. Analysis of HMW-GS composition of two Aegilops diploid species based on relative mobility[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(8): 178-182.

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