Isolation and identification of a pathogen of walnut black spot in Yichang city

doi:10.14088/j.cnki.issn0439-8114.2019.18.013

Abstract

Abstract: To identify the pathogen causing walnut black spot in Yichang city, the pathogenicity was determined according to Koch's rule. And the pathogenic bacteria was identified by morphological characteristics and rDNA-ITS sequence analysis. The results showed that Alternaria altenata was the pathogen causing walnut black spot in Yichang city.

Key words: walnut black spot, isolation and identification, morphological observation, Alternaria alternata, Yichang city

CLC Number:

S436.64

WANG Peng-cheng, WU Chu, SUN Zhi, PENG Xin, HAN Yong-shi, SUN Chang-cheng, JIA Qie. Isolation and identification of a pathogen of walnut black spot in Yichang city[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(18): 55-57.

References 16

[1]	陈善义,陶万强,王合,等.北京地区核桃黑斑病病原菌的分离、致病性测定和16S rDNA序列分析[J].果树学报,2011,28(3):469-473.
[2]	张永强,朱惠英,冯强,等.核桃黑斑病病原研究与防治试验初报[J].甘肃林业科技,2004,29(2):41-42.
[3]	阳金华,罗智勇,邓学基,等.鄂西北核桃主要病虫害调查及防治初探[J].湖北林业科技,2014,43(2):32-34,81.
[4]	李海菊,郝新科,牛昉卿,等.核桃黑斑病发生与防治[J].河北果树,2004(5):44-45.
[5]	李侠. 核桃黑斑病发生特点及防控措施[J].黑龙江农业科学,2014(10):172.
[6]	宫永红. 核桃细菌性黑斑病研究进展[J].北方果树,2012(6):1-4.
[7]	黄友佳. 宜昌市木本油料产业发展调查研究[J].绿色科技,2018(3):183-185.
[8]	方中达. 植病研究方法[M].第3版.北京:中国农业出版社,1998.
[9]	张天宇. 中国真菌志第十六卷链格孢属[M].北京:科学出版社,2003.33-42.
[10]	张荣,岳海梅,孙广宇.小孢子链格孢OPA2-1核苷酸序列分析及系统发育研究[J].菌物学报,2009,28(4):553-557.
[11]	崔迪,王继华,陈捷,等.链格孢属真菌对农作物的危害[J].哈尔滨师范大学自然科学学报,2005,21(3):87-91.
[12]	BELISARIO A,MACCARONI M,CORAMUSI A,et al.First report of Alternaria species groups involved in disease complexes of hazelnut and walnut fruit[J].Plant disease,2004,88(4):426.
[13]	HONG S G,MACCARONI M,FIGULI P J,et al.Polyphasic classification of Alternaria isolated from hazelnut and walnut fruit in Europe[J].Mycological research,2006,110(11):1290-1300.
[14]	MORAGREGA C,ZAKTAN H.Apical necrosis of Persian (English) walnut (Juglans regia):An update[J].Journal of plant pathology,2010,92(S1):67-71.
[15]	曲文文,刘霞,杨克强,等.山东省危害核桃的链格孢属真菌鉴定及其系统发育[J].植物保护学报,2012,39(2):121-128.
[16]	曲文文,杨克强,刘会香,等.山东省核桃主要病害及其综合防治[J].植物保护,2011,37(2):136-140.