Study on optimization of extraction process of Bupleurum chinensis saponins by central composite design-response surface methodology

doi:10.14088/j.cnki.issn0439-8114.2021.06.023

Abstract

Abstract: On the basis of single factor experiment, the effect of extraction times on the content of saponins in Bupleurum chinense was analyzed. The extraction process of saikosaponins was optimized by response surface method of central composite design. The effects of extraction time, extraction temperature and the ratio of liquid to material on saikosaponins were investigated. The results showed that the best conditions of total saponins of Bupleurum chinense were extraction temperature 80 ℃, extraction time 60 min, liquid-to-material ratio 8∶1（mL∶g）, and extraction twice. Under the optimal condition, the content of total saponins of Bupleurum chinense was 1.80 mg/g, which was well fitting with the predicted value of 1.81 mg/g. The extraction process by response surface methodology is accurate, reliable and predictable.

Key words: Bupleurum chinense, saponins, CCD-response surface methodology, extraction technology

CLC Number:

R282

ZHAO Yan-dan, ZHAO Yan-jie, LI Xiao-yang, SONG Li-hua. Study on optimization of extraction process of Bupleurum chinensis saponins by central composite design-response surface methodology[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(6): 110-114.

References

[1] 张爱华,郭姗姗.柴胡的临床运用及药理研究[J].中医研究,2019,32(4):7-9.
[2] 沈映君. 中药药理学[M]. 北京: 人民卫生出版社, 2004.
[3] LI C,HUANG L H,SUN W,et al.Saikosaponin D suppresses enterovirus A71 infection by inhibiting autophagy[EB/OL]. Signal transduction and targeted therapy,http://doi.org/10.1038/s41392-019-0037-x,2019.
[4] ZHU Y H,CHEN X B,RAO X L,et al.Saikosaponin a ameliorates lipopolysaccharide and D-galactosamine-induced liver injury via activating LXRα[J]. International immunopharmacology,2019,72:131-137.
[5] ZHAO L W,LI Q,XING X,et al.A novel enteromorpha based hydrogel optimized with Box-Behnken response surface method: Synthesis, characterization and swelling behaviors[J]. Chemical engineering journal,2016,287:537-544.
[6] SONDHI S,SAINI K.Response surface based optimization of laccase production from Bacillus sp. MSK-01 using fruit juice waste as an effective substrate[J]. Heliyon,2019,5(5):e01718.
[7] 郭梦鸿,孙玉琦,刘影,等.Plackett-Burman联合星点设计优选甜瓜蒂中葫芦素的提取工艺[J].中成药,2015,37(9):1937-1941.
[8] 张丹,陶燕铎,颜学伟,等.青海产小叶黑柴胡总皂苷提取工艺的优选[J].湖北农业科学,2014,53(5):1139-1141.
[9] 张国松,封传华,罗晓健,等.柴胡总皂苷提取工艺的优化[J].中国实验方剂学杂志,2011,17(12):17-20.
[10] 国家药典委员会. 中华人民共和国药典:2015 年版一部[S].
[11] 王冉冉,蒋子敬,谢凌宇,等.响应面法优化棕榈油基焙烤专用脱模剂制备工艺及其脱模特性[J].食品工业科技,2017,38(12):207-213.
[12] 刘鑫,邓姣,李洁欣,等.白藜芦醇/紫胶钠盐微胶囊的响应面法优化及其释放性能[J].食品工业科技,2019,40(17):121-126.
[13] 肖功胜,杨云,孙维英,等.柴胡有效成分提取工艺的研究[J].时珍国医国药,2008(8):1829-1831.
[14] ZHAO S, KWOK K C, LIANG H H.Investigation on ultrasound assisted extraction of saikosaponins from Radix Bupleuri[J]. Separation and purification technology,2006,55(3):307-312.
[15] 卫昊,刘清,卫伟光,等.HPLC法测定秦岭柴胡及其不同提取物中柴胡皂苷a和柴胡皂苷d[J].中成药,2013,35(2):342-345.