Population structure analysis of Dioscorea alata in Dabie Mountains based on SRAP markers

doi:10.14088/j.cnki.issn0439-8114.2022.09.031

Abstract

Abstract: In order to determine the population structure and genetic relationship of Dioscorea alata resources, SRAP molecular markers were used for marker detection of 48 Dioscorea alata resources collected in Dabie Mountains. The marker data were input into Structure 2.3.4 and the mixed model was used for population structure analysis. Twenty-two pairs of SRAP primer combinations with polymorphism were screened for marker detection of 48 Dioscorea alata resources. The results showed that a total of 175 polymorphic bands were detected in 22 SRAP primer combinations, with 4~11 polymorphic bands per primer combination and 7.95 polymorphic bands per primer combination on average. Population structure analysis showed that when K = 3, the △K value was the largest-that is, the 48 Dioscorea alata resources could be divided into three subgroups, including 15, 13 and 20 Dioscorea alata resources, and there was gene exchange between subgroups, which belonged to mixed subgroup. The population structure of 48 accessions of Dioscorea alata resources based on SRAP markers revealed that the genetic diversity of Dioscorea alata resources in Dabie Mountains was rich and the genetic relationship was complex. Divided into three subgroups, gene exchange exists between subgroups.

Key words: Dioscorea alata, SRAP, genetic diversity, population structure

CLC Number:

S632.1

LI Ren-xue, JIANG Ling, GU Qin, LI Shi-sheng, LI Jing-cai. Population structure analysis of Dioscorea alata in Dabie Mountains based on SRAP markers[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(9): 156-161.

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