Isolation, identification and whole genome sequence analysis of a porcine epidemic diarrhea virus variant strain

doi:10.14088/j.cnki.issn0439-8114.2024.07.022

Abstract

Abstract: PED was suspected to have occurred in a pig farm in Jiangsu Province, and after collection of disease material, PEDV was tested positive by RT-PCR. Virus-infected Vero cells were able to cause significant cytopathic changes, and the immunofluorescence results showed that the virus-infected cells could be recognized by a specific monoclonal antibody against PEDV N protein. Through whole genome sequence analysis, this strain was identified as a PEDV G2b subtype variant and designated PEDV JS-B strain.

Key words: porcine epidemic diarrhea virus, isolation and identification, variant strain, segmental sequencing, sequence analysis

CLC Number:

S852.65

LIU Xi, JIA Xiang-chao, LI Chen-xi, LI Zi-li. Isolation, identification and whole genome sequence analysis of a porcine epidemic diarrhea virus variant strain[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(7): 134-140.

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