Optimization of extraction of luteolin from millet pepper by response surface methodology

doi:10.14088/j.cnki.issn0439-8114.2024.07.023

Abstract

Abstract: With millet pepper as raw material, ultrasonic-assisted extraction was used to extract luteolin, and the extraction rate of luteolin was used as the index. The effects of material to liquid ratio, ethanol concentration, extraction time and extraction temperature on the extraction rate of luteolin were investigated through single factor experiment, and the process was optimized by the response surface analysis method. The results showed that the optimum extraction condition of luteolin from millet pepper was extraction temperature of 52.3 ℃, material to liquid ratio of 1∶50, ethanol concentration of 97%, and extraction time of 20 min. Under this extraction condition, the extraction rate was 1.641%, with a deviation of -0.061% compared with the theoretical value. The antibacterial activity of luteolin was tested by the Oxford cup method. The results showed that luteolin extract had a bacteriostatic effect on Staphylococcus aureus and Bacillus subtilis, but had no bacteriostatic effect on Escherichia coli.

Key words: millet pepper, luteolin, ultrasonic extraction method, response surface methodology, antibacterial

CLC Number:

TQ28

XIE Jun-quan, FENG Qing-ling, XIE Yong-wu. Optimization of extraction of luteolin from millet pepper by response surface methodology[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(7): 141-146.

References

[1] 王伟,何平,江小明. 木犀草素及其黄酮苷的抗炎、抗氧化作用[J]. 食品科学,2020, 41(17): 208-215.
[2] EL-ASFAR R K, EL-DERANY M O, SALLAM A A M, et al. Luteolin mitigates tamoxifen-associated fatty liver and cognitive impairment in rats by modulating beta-catenin[J]. European journal of pharmacology, 2021.DOI: 10.1016/j.ejphar.2021.174337.
[3] REUDHABIBADH R,BINLATEH T,CHONPATHOMPIKUNLERT P,et al.Suppressing Cdk5 activity by luteolin inhibits MPP+-induced apoptotic of neuroblastoma through Erk/Drp1 and Fak/Akt/GSK3β pathways[J]. Molecules, 2021,26(5):1307.
[4] 王春红,杨璐,胡敏,等. 乌拉草提取液中木犀草素含量的测定及其抗菌性能[J]. 纺织学报,2021,42(4): 114-120.
[5] ATTIQ A, JALIL J, HUSAIN K, et al.Luteolin and apigenin derived glycosides from Alphonsea elliptica abrogate LPS-induced inflammatory responses in human plasma[J]. Journal of ethnopharmacology, 2021, 275: 114120.
[6] FASOULAKIS Z, KOUTRAS A, SYLLAIOS A, et al.Breast cancer apoptosis and the therapeutic role of luteolin[J]. Chirurgia (Bucharest, Romania : 1990),2021, 116(2):170-177.
[7] SWAMINATHAN A, BASU M, BEKRI A, et al.Corrigendum: The dietary flavonoid, luteolin, negatively affects neuronal differentiation[J]. Frontiers in molecular neuroscience, 2019, 12: 122.
[8] 王智儀,郭佳佳,曹智威,等. 花生壳中提取木犀草素及其含量测定[J]. 生物化工,2021, 7(3): 61-63,67.
[9] 唐付杰,王星敏,何孟阳,等. 响应面法优化水热酸控提取紫苏梗中木犀草素工艺[J]. 食品工业科技, 2021, 42(17): 194-200.
[10] 赵丹,邓祖磊,崔田,等. HPLC法测定不同产地白毛夏枯草中木犀草素的含量[J]. 海峡药学, 2016, 28(11): 77-79.
[11] 王超,林娇,周建新. 乙醇回流法提取花生壳中木犀草素工艺条件优化[J]. 粮食与食品工业, 2013,20(6): 29-32,36.
[12] 冯亚莉,曹智威,顾悦馨,等. 连续萃取法从花生壳中提取木犀草素的研究[J]. 食品研究与开发, 2021, 42(10): 93-99.
[13] 秦梅颂,周国梁,张丽娟,等. 响应面法优化凤尾草中木犀草素的酶法提取工艺[J]. 中成药, 2017, 39(12): 2622-2624.
[14] 雷永平,钟方丽,王晓林,等. 离子液体辅助提取刺玫果中木犀草素与金丝桃苷的工艺研究[J]. 经济林研究, 2018, 36(3): 142-150.
[15] 高山,程丹,胡平. 万寿菊木犀草素提取工艺优化研究[J]. 内蒙古师范大学学报(自然科学汉文版),2012,41(6):644-648.
[16] 钟方丽,薛健飞,刘威振. 超声波辅助法提取锦灯笼宿萼中的木犀草素[J]. 食品与机械, 2012,28(1): 171-174.
[17] 孙长花,王征远,刘俊,等. 八角茴香提取液的抑菌效果及稳定性探讨[J]. 食品工业, 2020, 41(12): 189-192.
[18] HEMWIMON S,PAVASANT P,SHOTIPRUK A.Microwave-assisted extraction of antioxidative anthraquinones from roots of Morinda citrifolia[J]. Separation and purification technology,2007,54(1): 44-50.
[19] 朱德艳. 乙醇回流法提取葛根黄酮的工艺优化研究[J]. 中国酿造,2015, 34(11): 145-148.
[20] 王定美,王伟,李勤奋,等. 干燥温度对2个品种木薯嫩茎叶黄酮提取率的影响[J]. 农业资源与环境学报, 2017, 34(1): 86-94.
[21] 刘奉强,肖鉴谋,刘太泽. 应用响应面法优化超声波提取荆芥中总黄酮的工艺[J]. 南昌大学学报(工科版), 2011, 33(2): 149-155.
[22] 杨洋,聂静然,关红艳,等. 花生壳木犀草素的提取及其抑菌性能研究[J]. 食品科技,2009,34(12): 211-216.
[23] 王倩,谢明杰. 木犀草素对金黄色葡萄球菌的抑菌活性及其机制[J]. 微生物学报,2010,50(9): 1180-1184.