Study on the SSR genetic diversity of Camellia nitidissima var. phaeopubisperma

doi:10.14088/j.cnki.issn0439-8114.2026.01.017

Abstract

Abstract: To explore the genetic diversity of the Camellia nitidissima var. phaeopubisperma germplasm resources, a total of 111 samples of Camellia nitidissima var. phaeopubisperma from the same habitat with diverse phenotypic traits were analyzed using SSR molecular markers. The results showed that the 32 pairs of primers detected a total of 224 alleles (Na) across the 111 samples, with an average of approximately 7 alleles per locus. The total number of effective alleles (Ne) was 95.22, and the average effective allele number was 2.976. The average values of observed heterozygosity (Ho), expected heterozygosity (He), and the Shannon index (I) were 0.475, 0.562, and 1.194, respectively. The average polymorphism information content (PIC) was 0.528. Analysis of molecular variance (AMOVA) showed that 9% of the total genetic variation originated among populations, while 91% originated within populations (20% among individuals and 71% within individuals). The clustering results obtained from the unweighted pair group method with arithmetic mean (UPGMA), principal coordinate analysis (PCoA), and STRUCTURE analysis were largely consistent. All three methods classified the 111 samples into two genetic groups, and their phenotypic characteristics aligned with previous findings. This further confirmed that the diversity in leaf phenotypic traits of Fangcheng Camellia nitidissima was closely associated with its genetic background.

Key words: Camellia nitidissima var. phaeopubisperma, genetic diversity, SSR, genetic structure

CLC Number:

LI Shuang-shuang, GAO Hui, SU Meng-xue, CHEN Jin-mei, XIE Lei-hui, HUANG Yong-guang, LIN Xing, DENG Jia-gang, XIE Yang-jiao. Study on the SSR genetic diversity of Camellia nitidissima var. phaeopubisperma[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(1): 97-104.

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