Abstract: In order to accurately determine the content of vitamin K₁ in samples, a highly sensitive detection technology for vitamin K₁ in Chinese cabbage （Brassica rapa L. ssp. pekinensis） based on high performance liquid chromatography-fluorescence detection was established. The optimized mobile phase and hexane vortex were used to extract the samples to be tested. After purification by the neutral alumina column, the samples were quantitatively analyzed by the C₁₈ reverse-phase chromatography column. The results showed that vitamin K₁ had a good linear relationship within the range of 0.229~6.197 μg/mL, and the correlation coefficient（R²） was 0.996 9. The detection limit of this method was 0.29 μg/100 g, and the limit of quantification was 0.95 μg/100 g. Recovery rate was 97.5%~101.8%. This method had the advantages of early peak time, effective separation of miscellaneous peaks and high detection limit, which was the optimal method for the detection of vitamin K₁ in Chinese cabbage.

Key words: vitamin K₁, Chinese cabbage（Brassica rapa L. ssp. pekinensis）, solid phase extraction, liquid chromatography