HUBEI AGRICULTURAL SCIENCES

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Progress of ecological control techniques for eutrophication of lakes in China

LIU Han, WANG Han-xi, SHENG Lian-xi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 5-6. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.001

Abstract （733）

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Eutrophication of lake water in China is serious, and ecological control has attracted widespread attention because of its no pollution and small environmental impact. Ecological control technology of the lake water rich nutrition were summarized. It is pointed out that it is difficult to control the lake ecology in northern China, and the effect of using biochar with reproducibility as substrate to repair eutrophication water body is good. In addition, the effects of complex constructed wetland and three-dimensional ecological floating bed technology on eutrophic water body are remarkable. At present, aquatic plants and substrate materials play an important role in eutrophic restoration of lakes, but the utilization rate of plant biomass is low. Especially, the matrix materials have not been used as resources after retirement. In the future, we should pay attention to improving the level of eutrophication and ecological control of lakes, realize the utilization of resources and reduce the cost of lake control.

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Overview of well-being in domestic tourism

MENG Cai-juan, LIU Chao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 11-16. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.002

Abstract （597）

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As an important activity related to human well-being, tourism has become indispensable in people's daily life, and tourism happiness has become more and more concerned by scholars. These results were found by reviewing the relevant research results in the past 20 years. ①The domestic tourism well-being literature first appeared in 2009, and after 2013, tourism happiness was more concerned. ②There were three major areas of tourism well-being research, the well-being of residents in tourism communities, the well-being of tourists （including subjective well-being, psychological well-being）, and their influencing factors. ③In the context of tourism economy, some scholars believed that the well-being of tourists and the well-being of residents in tourist destinations were the perception of the influence of tourism in the process of participating in tourism. ④The well-being of tourists was composed of the emotions and values of the three periods before, during and after the tour, there was no clear analysis dimension for the composition of community residents' happiness.⑤There were population differences in the influence of community participation, gender, age, education, and income on tourism well-being.

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Research on the application of big data in intelligent agriculture

CHEN Ying-bo

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 17-21. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.003

Abstract （546）

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The processing flow of big data in intelligent agriculture was analyzed, the overall framework of agricultural big data was designed, the application of big data collection technology, management technology and processing technology in the field of agriculture were explored and these application were studied with specific examples. It is of great practical significance and application value to promote the transformation and upgrading of agriculture and speed up the development of intelligent agriculture.

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Effect of different fertilizers on the yield and yield-related traits of sesame variety Ezhi No.6

TANG Xue-hui, TAN Shun-lin, LIU Ke-zhao, CHEN Han-jun, MU Jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 22-24. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.004

Abstract （517）

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To get simple and efficient formula fertilization, sesame （Sesamum indicum Linn.） variety Ezhi No.6 was adopted to study the effect of different N, P, K fertilizers on sesame yield and yield-related traits by “3414” fertilizers application experiment. The results showed that the different levels of N, P, K have different effects on the yield of sesame, and the nitrogenous fertilizer had the highest effects. Among 14 treatments, the yield of treatment of 292.5 kg/hm² urea, 249.0 kg/hm² superphosphate and 150.0 kg/hm² muriate of potash kalium chloratum （N₃P₂K₂） was the highest, and the yield was 1 751.0 kg/hm²; The yield of treatment of 195.0 kg/hm² urea, 124.5 kg/hm² superphosphate and 150.0 kg/hm² muriate of potash kalium chloratum （N₂P₁K₂） was the second, and the yield was 1 682.0 kg/hm²; The yield of no fertilization treatment（N₀P₀K₀） was the lowest, and the yield was 1 302.0 kg/hm². The optimum ratio of N, P and K is 10∶2∶7.

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Preliminary study on cultivation patterns and effect of high-starch sweet potato varieties

YE Xing-zhi, CHEN Qiao-ling, QU Yong, XU Yi, HAO Miao, ZHANG Deng-hong, WANG Zhen, CHENG Qun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 25-27. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.005

Abstract （421）

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In order to explore the high yield cultivation mode of high starch sweet potato [Ipomoea batatas （Linn.） Lamarck], the effects of four factors including different fertilizers, sweet potato cultivars, planting densities and mulching types on vine yield, fresh root tuber production, dry matter rate and T/R value（ratio of fresh vine yield and fresh root tuber production）were studied by an orthogonal randomized test. The results showed that combination A₃B₁C₃D₂, that is, applying compound fertilizer, choosing Shangshu 19, covering black film, planting density of 59 895/hm², had the highest production of fresh root tuber. The vine yield, dry matter rate and fresh root tuber production of compound fertilizer was the highest; The effect of sweet potato cultivar on vine and T/R value was the most remarkable; Mulching can improve dry matter content and the production of haulm and tuber; And the effect of cultivation density on dry matter content was the highest.

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Comparative analysis of oilseed and vegetable dual-purpose rapeseed varieties based on yield and economic benefits

HUANG Wei, CHANG Hai-bin, LI Ning, HU Hai-zhen, YIN Hui, ZHAO Jun-li

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 28-31. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.006

Abstract （477）

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Seven oilseed and vegetable dual-purpose rapeseed （Brassica napus L.） varieties from different sources were used as materials, and under the suitable cultivation conditions, the same experimental treatment was carried out, and the varieties were compared and analyzed based on the indexes of rapeseed stalk yield, rapeseed yield and economic benefits. The aim was to provide the basis for the promotion of high-quality rapeseed varieties of oilseed and vegetable dual-purpose. The results showed that Fenglu No.2, Youtai 929, Fenglu No.1 and Shishancaitai were suitable for the promotion and application as high-quality rapeseed varieties of oilseed and vegetable dual-purpose.

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Microspore culture and double haploid seed setting analysis in three rapeseed F₁ hybrids

WAN Li-li, WANG Zhuan-rong, YANG Guang-sheng, XIN Qiang, HONG Deng-feng, SUN Yu-hong, GAO Hong-xia

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 32-38. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.007

Abstract （378）

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To investigate the ploidy levels of microspore-derived plants and effect of colchicine and trifluralin treatment on regeneration, double haploid, and seed setting of regenerated plants in oilseed rape （Brassica napus L.）, the effects of microspore culture treatment with antimitotic agents colchicine and trifluralin on the frequency of embryo formation, embryo development, plant regeneration and diploidization rate in three F₁ hybrids of rapeseed cultivars were compared, and the ploidy levels of young plantlets were measured by flow-cytometry （FCM）. The results indicated three F₁ combinations obtained the most number of regenerated plantlets after treatment with 85 mg/L colchicine. The mean rates of DH plants from colchicine（85 mg/L） were higher than the other colchicine concentration, and maximum DH plants were derived from the trifluralin treatment（10 μmol/L）. After 4 ℃ cold treatment with embryos derived from three F₁ hybrid combination for 10 days, the number of embryos to plants conversion （EPC） was significantly increased. The fruity characters of DH population obtained by natural doubling and doubling agent of three F₁ combinations were investigated, and the results showed that the average pod length,pod number and 1 000-grain weight of double haploid plants treated from antimitotic agents was similar to the spontaneous double haploid plants.

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Experimental study on purification of rural domestic sewage： A case of Cencun river,Guangzhou city

GAO Ting, ZHANG Zhen-bang, WU Qi-tang, ZHENG Jia, SHI Jia-cheng, WEI Ze-bin, WU Wei-bin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 39-43. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.008

Abstract （480）

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In response to the difficulties in rural sewage treatment, high investment costs in infrastructure, and complex technology, the static test of wastewater from sewage treatment in the Cencun river, Guangzhou city, was carried out to simulate the discharge time of rural household sewage, and the treatment of sewage with the planting of Pennisetum sinese Roxb and Canna generalis L. was conducted. The results showed that compared with the control treatment, the removal rates of ammonia nitrogen, total nitrogen （TN） and total phosphorus （TP） of planting Pennisetum sinese Roxb and Canna generalis L. increased by 43.01,41.70 and 61.62, 60.44 percent, respectively, and the treatment of Pennisetum sinese Roxb was better than the treatment of planting Canna generalis L.. The removal rates of COD, TP, ammonia nitrogen, and TN in wastewater of Pennisetum sinese Roxb were 79.86%, 99.83%, 98.86% and 98.14%, respectively, while the Canna generalis L. was 57.51%, 98.65%, 98.18% and 96.83%, respectively.

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Comparison of key technologies for rural domestic sewage treatment in Jiangxi province

CAO Teng-fei, KONG Qiong-ju, LU Jiang-hai

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 44-48. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.009

Abstract （415）

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The choice of rural domestic sewage treatment technology and process is related to the construction, operation and maintenance costs of sewage treatment facilities. In order to implement the notice of the General Office of the Central Committee of the Communist Party of China on printing and distributing Three-Year Plan for Rural Residential Environment Remediation,and improve the level of rural domestic sewage treatment, begining with the current situation of rural domestic sewage treatment at home and abroad, the discharge characteristics and existing problems of rural water environmental in Jiangxi province were sorted out, a comparison on the key technologies of rural domestic sewage treatment was made, and corresponding suggestions were put forward.

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A remediation plan for black and odorous river in a township in the suburbs of a city of south Jiangsu

XUE Jing-jing, WANG Mei-juan, YU Xin-ya, MAO Lin-qiang, ZHANG Wen-yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 49-53. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.010

Abstract （429）

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In recent years, the urbanization of southern Jiangsu province has developed rapidly, turning the towns and towns far away from the city into unplanned “urban suburbs”. At the same time as the rapid development of towns and villages, increasing sanitary sewage, industrial wastewater and initial rainwater which makes the water worse are the causes of black and odorous rivers. This project took the black and odorous river in the suburbs of a city in southern Jiangsu as an example. After a field investigation of the river, the river was divided into five parts according to biological phase, sediment, water quality and integrating the water landscape design concept of Jiangnan villages and towns. Each part adopted different physical-bio-ecological combination processes （desilting, interception, re-oxygenation, wetlands produce running water, ecoenhancement） for design and construction. Engineering test results showed that the remediation plan can have higher removal efficiency on pollutants including ammonia nitrogen （NH₃-N）, total nitrogen（TN）, total phosphorus（TP）, chemical oxygen demand （COD）, etc. in heavy polluted rivers. NH₃-N, TN, TP, COD can maintain its concentration at 1.5 mg/L, 2.0 mg/L, 0.3 mg/L, 34.7 mg/L, removal rates were 66.52%, 79.21%, 44.44%, 42.55% when 6 months after completion of the construction. At the same time, dissolved oxygen （DO）, transparency and redox potential in the river were stable at 5 mg/L, 60 cm, 500 MV. The quality of the river has reached the requirements of Class V of the Surface Water Environmental Quality Standards. After public comments, black and odorous rivers will be eliminated to create a natural, lively and beautiful ecological landscape of the town. According to the calculation, the total investment of the project is about 1.2 million, the annual operating cost is 61 000, and the emission reductions of NH₃-N, TN, TP and COD are up to 36.09 t/a, 92.28 t/a, 2.91 t/a, 310.86 t/a,respectively.

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Characteristics of soil water-aggregates in rain-fed drylands in the plain of Yili valley

CHEN Chuan-xin, ZHANG Yong-qiang, XUE Li-hua, LEI Jun-jie, CHEN Xing-wu, XIAMAIDING Abasi, LIU Yun-xiao, LIU Xin-yan, WU-Wei, KULEDANA Kadaerjiang, SAILIHAN Sai

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 54-57. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.011

Abstract （497）

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The characteristics of soil aggregates under different yield levels in dry wheat fields in Xinjiang were analyzed by wet sieve method. The results showed that there were differences in the size of water stable aggregates in soil at different yield levels. The soil aggregates of >5 mm particle in high-yield field soil were significantly higher than those of medium-yield and low-yield fields, increased by 45.18% and 52.72%, respectively; The soil aggregates of 0.25~0.5 mm particle in high-yield fields and medium-yield fields were significantly higher than those in low-yield fields, increased by 45.70% and 51.15% respectively; The soil aggregates of <0.25 mm grain in low-yield fields increased significantly by 36.34% and 8.84% compared with high-yield fields and medium-yield fields. The content of large aggregates in the soil of >0.25 mm particle size in high yield field reached 68.30%, increased by 13.29% and 20.29% respectively over the middle and low yield fields. The mean weight diameter （MWD） and geometric mean diameter （GMD） of soil all showed that high-yield fields was the highest, medium-yield fields was second, and low-yield fields was the lowest. But the fractal dimension is opposite. The fractal dimension of soil was significantly negatively correlated with MWD, GMD and the soil aggregates of >5 mm particle, and was significantly positively correlated with <0.25 mm particle. MWD, GMD were significantly positively correlated with the soil aggregates of >5 mm particle, and significantly negatively correlated with the soil aggregates of <0.25 mm particle.

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Improvement of coastal saline soil by organic material and its effect on the growth of Lonicera maackii

FNEG Tao, ZHOU Guo-hua, LU Li-bo, SU Xiao, LIU Wei, LYU Le-fu, YOU Xue-yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 58-61. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.012

Abstract （432）

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To explore the effects of organic materials on the improvement of coastal saline soil, cow dung, vinegar grains, edible fungi residue and plant crushing waste were selected as materials. 14 kinds of proportions were set for saline soil treatment, and Lonicera maackii were planted for growth test, and the total salt content and pH of soil were determined regularly. The results showed that organic materials had different effects on the improvement of soil salt content and pH, in which, the vinegar residue, mushroom residue and green crushing waste mixed with a small amount of organic fertilizer, i.e. cow manure∶vinegar residue∶mushroom residue∶green crushing waste was 1∶3∶3∶3（volume rato）, which had better effect on soil improvement, and the maximum growth of Lonicera maackii could be obtained under this condition. In evaluating the effect of soil improvement, the growth index（plant height, crown width） is more representative than the physiological index （chlorophyll index and net photosynthetic rate）.

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Multi-functional evaluation of county land use based on the perspective of ecological civilization：A case study of Guiping city,Guangxi

MO Su-fen, WU Zhuang-jin, HU Bao-qing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 62-66. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.013

Abstract （463）

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17 indexes from production, living and ecology three dimensions were selected to establish evaluation index system based on the perspective of ecological civilization. Index weight was determined by entropy evaluation method and comprehensive evaluation model was set up to evaluate land use multi-functional of Guiping city, Guangxi, from 2000 to 2017. The results showed that the overall level of multi-functional of land use was not high in Guiping city, showing a trend of first decreasing and then increasing. The level of production functional of land use was not high, showing a trend of first stationary and then gradually increasing. The level of living functional of land use was medium and upward, showing an upward trend of fluctuation. The level of ecological function of land use was relatively high, showing a trend of first decline and then rise. It is suggested to increase the input of productive land, control the scale of living land and increase the proportion of protected areas and other measures to improve the level of multi-functional of land use.

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Interdecadal variability of spring sea surface temperature in and around the South China Sea and its possible impact on climate in the Indo-Pacific region

GAO Wen-juan, MENG Lei, LUO Yu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 67-73. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.014

Abstract （505）

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In order to study the main characteristics, formation mechanism and seasonal trends of spring climate in and around the South China Sea in the context of global warming, the main modes of spring sea temperature in and around the South China Sea in the context of the inter-generational context were analyzed, and their possible effects on spring climate in the Indian Ocean and the Pacific region during the same period were analyzed by the methods of empirical orthogonal function （EOF） decomposition, linear tendency estimation, sliding average and correlation analysis. The results show that in and around the South China Sea, the spring sea temperature anomaly presents two modes. The first mode is consistent warming in the region, and the second mode is cold in the north and warm in the south. The first modal sea temperature distribution is significantly affected by global warming. In the spring, there is an abnormal anti-cyclone circulation in the Philippine Sea area, and the western side of the subtropical high is strengthened. The consistent warming of the Indian Ocean basin in the previous winter indicate that the spring will be consistent warming in and around the South China Sea in the following spring. The second mode is obviously affected by the La Niña event in the pre-winter Pacific Ocean. The South China Sea temperature anomaly triggers a positive and negative wave distribution of the potential height field on the East Asian coast. The first （second） mode of precipitation in and around the South China Sea shows the distribution of waterlogging （drought） in the north and drought （waterlogging） in the south. The abnormal sea temperature in the South China Sea and its surrounding areas in spring will have a significant impact on the spring and summer climate in the India-Pacific coastal areas.

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Regression analysis of Morus alba leaf size and its mass

JIN Fang-lun, WANG Xian-yu, HAN Shi-yu, LUO Chan-bin, YANG Sheng-te, LI Ming, HU Shi-yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 74-77. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.015

Abstract （662）

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In order to explore the main factors affecting the net quality of mulberry （Morrus alba L.） leaves, from 2017 to 2018, Nongsang 14 was used as a test material, the leaf length, leaf width, petiole length, petiole width and transverse diameter, petiole thickness and transverse diameter, petiole mass, leaf mass, number of kilogram leaves, and leaf net mass were investigated in the growing season to analyze the relationship of mulberry leaf characteristics. The results show that there is a highly positive correlation between leaf length and leaf width and leaf net mass. The petiole length, petiole width and transverse diameter, and petiole thickness and transverse diameter are significantly positively related to the leaf net mass. There are extremely significant differences between the correlation coefficient of leaf length, leaf width, leaf length + leaf width, leaf length × leaf width, petiole length, petiole width and transverse diameter, petiole thickness and transverse diameter, petiole mass, leaf mass, kilo leaf quantity and leaf net mass. Comparing the correlation coefficients of the quadratic curve equation and the straight line equation formed by the blade length and the net mass of the blade, respectively, it shows that it is more in line with the quadratic curve. The order of influence of each index on the leaf net quality is leaf mass, leaf number per kilogram, leaf width, leaf length, petiole mass, petiole thickness and transverse diameter, petiole width and transverse diameter, and petiole length. Mulberry orchard yield can be estimated by investigating indicators such as leaf width and leaf length.

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Study on variation of flowering stalk height and its related traits in seed seedling of Hemerocallis hybridus Stella de Oro

LUO Xin-kai, LUO Zhen-xing, WANG Li, ZHU Hong, WANG Man, YUE Long

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 78-81. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.016

Abstract （429）

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The flowering stalk height, number of single stalk flowers, leaf length and the variation of flowering period of seed seedling in Hemerocallis hybridus Stella de Oro were studied. The results showed that the variation coefficient of number of single stalk flowers was the largest, the variation coefficient of flowering stalk height and leaf length were larger, and the coefficient of variation of flowering days was the smallest. The number of single stalk flowers and flowering stalk height of seed seedling were highly significantly more than their female parent. According to the calculation of the generalized heritability, the variation of flowering stalk height, number of single stalk flowers and leaf length were mainly controlled by genetic factors, the variation of flowering period mainly controlled by environmental factors. There was a high correlation between flowering stalk height, leaf length and the number of single stalk flowers. Cluster analysis was carried out on seed seedlings, which were divided into 4 groups, from the first group to the fourth group, the height of flower stalk changed from high to low, until there was no flower stalk. Correlation analysis was carried out in each group. In the first and second groups, there was a significant correlation between the height of flower stalk and leaf length, while the rest was negatively correlated. The correlation coefficient between flower stalk height and number of single-stalk flowers increased gradually from the first to the fourth groups, from low degree to high degree.

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Analysis of using value and protection measures of wild peony resources of Subsect. Delavayanae

WANG Er-qiang, PANG Jing-jing, JI Han-le

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 82-85. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.017

Abstract （433）

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The species and biological characteristics of wild peony resources of Subsect. Delavayanae were expounded,and the potential utilization value of medicinal, oil and breeding of wild peony of Subsect. Delavayanae were analyzed, besides,the protection countermeasures and rational utilization were also proposed. It is expected to provide reference for the effective protection and sustainable use of wild peony in the Subsect. Delavayanae.

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Analysis and evaluation on dynamic change of forest resources in Hubei province： Based on data of eight continuous inventory of forest resources

HUANG Guang-ti, CHEN Qiang, LIN Cheng-jun, SHEN Li-li, YU Xiang, CHEN Zhen, HUANG Xin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 86-90. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.018

Abstract （464）

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On the basis of explaining the construction of the continuous inventory system of forest resources in Hubei province, based on the data of eight consecutive forest resource inventories from 1979 to 2014, the dynamic changes of forest land area, coverage, and total standing tree standing volume in Hubei province were analyzed, and the change trend of forest resources in Hubei province was analyzed and evaluated. The results show that the area of forest land in Hubei province has maintained a substantial growth trend, but the space for future growth is limited; the forest coverage rate has shown a steady upward trend, but the growth rate will slow down; the total volume of standing timber has shown a rapid growth momentum, and the growth rate will stabilize in the future.

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Study on Tibetan medicinal ethnic botany in Banma area,Qinghai

ZHANG Jie, LONGZHU Duo-jie, KANG Jun-hua, LA Ben

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 91-94. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.019

Abstract （466）

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An investigation was conducted on the Tibetan medicinal ethnobotany in the Banma region of Qinghai. The results show that there are 478 Tibetan medicinal plants belonging to 228 genera of 70 families in the Banma area. The Tibetans are unique in terms of selecting medicinal plant species, medicinal parts, harvest season, processing and drinking methods. For the protection and sustainable use of medicinal plant resources in the region, corresponding suggestions were put forward.

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Comparison of biological characteristics and fruit quality of anthropogenic lines of Lycium barbarum

ZHANG Bo, LUO Qing, CAO You-long, DAI Guo-li, HUANG Ting

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 95-96. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.020

Abstract （427）

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Taking Ningxia wolfberry （Lycium barbarum） Ningqi No. 1 anther cultured strains as research objects, its leaves, flower organs, fruit biological characteristics investigation and fruit quality were studied to obtain breeding materials with good phenotype. The results show that the use of anther culture technology can create germplasm resources with different morphological characteristics. Compared with the parent plant, the leaf length becomes shorter, the leaf width and thickness of some lines increase, the diameter of the corolla and the length of the petiole decrease, the weight of the single fruit decreases, the aspect ratio decreases, and the number of seeds decreases. The content of carotene and betaine in fresh fruit reduces. Among the 5 anthropogenic lines, the fruit weight, aspect ratio, and sugar content of the material "194" were significantly higher than those of other materials.

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Effects of drought stress on potassium absorption and distribution of two flue-cured tobacco varieties

SUN Ji-ping, WU Zhao-hui, SUN Huan, LI Xue-jun, PING Wen-li, DING Yan-fang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 97-100. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.021

Abstract （409）

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In order to study the characteristics of potassium absorption and accumulation in different flue-cured tobacco varieties under drought stress, pot experiments were used to simulate natural drought condition, and the differences in potassium content and accumulation between 2 flue-cured tobacco varieties Yuyan 13 and Zhongyan100 were compared. The results showed that the potassium content of roots, stems and leaves of the two flue-cured tobacco varieties was the highest during the peak period, and decreased with the increase of biomass, the proportion of potassium in the root system increased at the mature stage, and the proportion of leaves decreased. The potassium content and accumulation of leaves and stems of Yuyan No.13 at maturity were higher than those of Zhongyan 100; under moderate drought conditions, the potassium content of Yuyan No.13 and Zhongyan 100 was higher than that of the control, and the potassium accumulation was lower than that of the control; under severe drought conditions, the potassium content and accumulation of stems and leaves of Yuyan No.13 were higher than those of the control, and that of Zhongyan 100 was lower than that of the control. Under drought conditions, the distribution ratio of potassium in roots and stems of Zhongyan 100 increased and the distribution ratio of potassium in leaves decreased. Drought stress had little effect on the potassium absorption and its distribution in rhizomes and leaves of Yuyan No.13.

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Effect of different topping times and remaining branch tobacco numbers on the quality of upper burley tobacco

JI Fei, WU Bao-cheng, YANG Dong-rong, MA Fang-lei, WEN Tao, ZHANG Fang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 101-105. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.022

Abstract （446）

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To study the effects of different topping periods and the number of leaves on the tobacco leaves on improving the quality of the upper leaves of burley tobacco, and determine the optimal topping period and the number of leaves on the tobacco leaves, plot trials were conducted in the core tobacco area of burley tobacco in Jianshi county, Enshi prefecture, Hubei province. The results showed that the combined measures of different topping periods and the number of leaves remaining on the tobacco can directly affect the economic benefits of tobacco leaves and the distribution of nicotine content in tobacco plants. At the early stage of budding, keeping 8 and 6 leaves of tobacco leaves can significantly increase the output value of tobacco leaves, but the nicotine content in the upper part and the branches is high, and the internal chemical components are not coordinated. In the initial flowering stage, 15%~20% flower topping tobacco, the yield of keeping 4 leaves was lower than that of the early budding with 8 and 6 leaves, but the nicotine content in each part was appropriate and the internal chemical composition was coordinated. Comprehensive analysis shows that under the treatment of keeping 4 leaves of tobacco leaves in the early flowering stage, the economic benefits of tobacco leaves are better, the nicotine content is appropriate, and the industrial availability of upper tobacco is stronger.

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Preliminary report on regional experiment of 7 different flue-cured tobacco varieties （strains） in Guizhou province

SU Chang-tao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 106-109. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.023

Abstract （517）

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To provide a reference for the approval, promotion and layout of new flue-cured tobacco varieties, taking G68 （K326） as a control, regional tests of flue-cured tobacco varieties （strains） G61, G62, G63, G64, G65, G66, G67 are conducted; and their agronomic and economic traits, including seedling growth, seed color, uniformity, plant shape, leaf shape, growth potential, growth period, disease resistance, tobacco leaf yield and quality, and economic benefits are investigated. The results showed that among the eight varieties （strains） tested, G66 had better agronomic traits and economic benefits, and G64 performed worse.

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Superovulation effect of Yichang white goat

CHONG Yu-qing, ZHU Fang-xian, SHEN Hong-xue, ZHU De-jiang, QIN Jun-gan, DENG Xiao-lin, YIN Chun-yun, LIU Pan, JIANG Xun-ping, LIU Gui-qiong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 110-113. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.024

Abstract （438）

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Yichang white goats were selected to study the effects of different FSH dosages on superovulation. The results showed that the rate of synchronization estrus was 100% and the number of corpus luteum, collected embryo and effectiveembryo increased with the increment of FSH dosage. When injected 18 AU FSH per goat, the corpus luteum, collected embryos and effective embryos of 6-month-old goats was 18.60, 15.60 and 14.20, respectively while of the 12-month-old goats was 20.00, 16.25 and 14.00, respectively. And the superovulation effects had no significant difference between 6-month- and 12-month- goats. It suggested that the Yichang white goat could be treated superovulation at 6-month-old and the suitable FSH dosage of superovulation for 6- and 12-months old goat was 18 AU/head.

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Analysis on drug resistance phenotype of Escherichia coli isolated from different pigs cultivation modes in Guangxi

FENG Shi-wen, LI Jun, PAN Yan, HE Hui-li, PENG Hao, ZHONG Shu-hong, HU Shuai, YANG Wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 114-118. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.025

Abstract （466）

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This study was to investigate the difference of drug resistance phenotype of E. coli isolated from different pigs cultivation modes in guangxi, and to provide a reference for the scientific use of antimicrobial drugs of swine colibacillosis and to slow down the drug resistant strains. E. coli from different pigs cultivation modes in Guangxi was isolated and identified, and the drug-resistant phenotype was tested and analysis. The results showed that, the E.coli isolates from three pigs cultivation modes were highly sensitive to cefoxitin, ceftazidime and amikacin, the sensitive rate up to 70%, while highly resistant to penicillin G, ampicillin, amoxicillin, rifampicin, lincomycin and sulfamethoxazole, the resistance rate up to 90%. The resistance rate of E.coli from large scale pig farms against enrofloxacin was extremely significantly lower than free-range farming （P<0.01）, the resistance rate of E.coli from large scale pig farms against ofloxacin, gentamicin, florfenicol were significantly lower than free-range farmers （P<0.05）. The resistance rate of E. coli from small and medium-sized pig farms against enrofloxacin, doxycycline and florfenicol were significantly lower than free-range farmers （P<0.05）. The resistance rate of E. coli from free-range farmers against ciprofloxacin, kanamycin, spectinomycin were extremely significantly higher than large scale pig farms and small and medium-sized pig farms（P<0.01）, while the resistance rate against cefotaxime was extremely significantly lower than large scale pig farms and small and medium-sized pig farms（P<0.01）. The multiple antibiotics resistance index （MARI） of E. coli from large scale pig farms, small and medium-sized pig farms and free-range farmers was 0.62, 0.63 and 0.68. 14 to 23 resistant E. coli strains accounted in free-range farming, which was extremely significantly higher than large scale pig farms（P<0.01）, and was significantly higher than small and medium-sized pig farms（P<0.05）. The results indicated that the resistance of E. coli from different pigs cultivation mode were different but all very serious and mainly of multi-drug resistance.

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Structural characterization of Ce-ZnO nanomaterials and photocatalytic degradation of rhodamine B dye

XU Jia-hao, CHEN Rou-lin, YANG Shu-yi, LI Meng-jie, SONG Ru, YU Qun-di

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 119-122. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.026

Abstract （447）

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Ce-doped nano ZnO nanomaterials were prepared by doping rare earth element Ce with nano-ZnO which prepared by pyrolysis at high temperature, and the structural characteristics and element composition of the materials was characterized by X-ray diffraction （XRD） and energy dispersive spectroscopy （EDS）. The effects of Photocatalytic Degradation of Dye RhB Catalyzed by Ce-ZnO was researched in Rhodamine B （RhB） dye as a single sewage system. The result of phase analysis of xray diffraction indicated that the Ce-ZnO catalyst has six crystal wurtzite type structure characteristic diffraction peak and had a diffraction peak of CeO₂. The result of energy spectrum showed that the mass ratio of Ce and Zn is 1∶23.88. Photocatalytic degradation of RhB by Ce-ZnO followed the first-orderkinetics, In natural light, the apparent rate constant of RhB degradation catalyzed by nano-material Ce-ZnO is 0.039 8, which is greater than the apparent rate constant under ultraviolet light （0.024 0）. It indicated that doping rare earth element Ce could effectively improve the catalytic degradation of RhB by nano-ZnO under natural light.

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Study on antioxidant activity of polysaccharide from Gomphidius rutilus

JIN Zhi-ming, CHAI Jun-hong, HE Ting-ting, PENG Dan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 123-125. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.027

Abstract （433）

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The antioxidant activity of polysaccharide which extracted from Gomphidius rutilus was studied by. in vitro antioxidant systems. The structure of the extracted polysaccharide was also studied by infrared spectroscopy. The results showed that when the polysaccharide concentration reached 1 mg/mL the scavenging rate of superoxide radical, DPPH and hydroxyl radical was 96.96%, 48.53% and 94.95%, respectively. The IR results showed that polysaccharide is beta-pyranose. It indicated that Gomphidius rutilus’s polysaccharide has a high antioxidant capacities and a bright future of development and utilization.

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Optimization of microwave-assisted extraction from Hibiseusmanihot L. by response surface methodology and its volatile components analysis

JIANG Mei-hong, LI Xian, ZHANG Bo, ZHENG Lin, WU Chang-wei, HUANG Zhi-qiang, PU Xing-wei, YANG Ji

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 126-130. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.028

Abstract （470）

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In order to study the development and application of Hibiseusmanihot L. in the fields of tobacco, flavor and fragrance industries,the process of microwave-assisted extraction to make tobacco flavor from Hibiseusmanihot L. was optimized by response surface methodology （RSM）, and its volatile components were analyzed and identified. Based on single factor experiment, with water addition at pretreatment, extraction time, extraction power as factors, and the yields of Hibiseusmanihot L.tobacco flavor as response value, the mathematical model was established by Box-Behnken Design（BBD）for response surface analysis. The volatile components were collected by simultaneous distillation and extraction device, analyzed and identified by gas chromatography-mass spectrometry. The results showed that the extraction yield of Hibiseusmanihot L.could be 0.290% when extracted 85 min 520 W power and the water addition at pretreatment was 1∶2.5. 45 components were identified and mainly of： 1-Docosene, β-Sitosterol, 9,12,15-Octadecatrienoic acid, n-Hexadecanoic acid, Acetic acid, n-octadecylester, 1,2-Benzenedicarboxylic acid, mono（2-ethylhexyl） ester, 26,26-Dimethyl-5,24（28）-ergostadien-3.beta.-ol, Stigmasterol, et al. It suggested that the microwave method could be used as an effective method to extract the flavor of Hibiseusmanihot L.

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Curcuma kwangsiensis germplasm different types of high volatile oil content by GC-MS analysis

YANG Ni, WANG Jian, XIE Wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 131-134. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.029

Abstract （460）

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Steam distillation method using GC-MS of different germplasm Curcuma kwangsiensis high volatile oil content of the composition analysis, determine its composition and contains volatile oil content of each component.The result showed that different germplasm Curcuma kwangsiensis high volatile oil content types there are both volatile oil from volatile oils, and there peculiar volatile oil components, the same composition of different germplasm resulting mass fraction varies, the number of detected peaks are large differences of detected in 31 kinds of compounds.The results of C-108,D-16,D-11,D-3 the four varieties. With different content of volatile oil contains chemicals and active ingredients there is a certain correlation between the results of this study can be volatile oil quality control and breeding new varieties Curcuma provide a scientific basis.

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Identification of Alpinia officinarum and Alpinia Galanga by HS-SPME-GC-MS

LI Jin-zhou, YANG Hong-bing, LIU Yi, CHEN Cheng, LUO Yu-shen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 135-138. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.030

Abstract （504）

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The volatile components of Alpinia officinarum and Alpinia Galanga were extracted by headspace solid phase microextraction （HS-SPME）, and analyzed by gas chromatography-mass spectrometry （GC-MS）, and the relative content of each component was calculated by the method of area normalization. The results showed that 128 peaks were detected, and 69 compounds were identified in two medicinal materials. In Alpinia officinarum, 61 peaks were detected, and 44 components were identified, which is account for 91.52% of the total volatile components. The main substances were1,2,4a,5,6,8a-Hexahydro-4, 7-dimethyl-1-（1-methylethyl）naphthalene, Eucalyptol, alpha-Terpineol, Seychellene, 1-Caryophyllene, beta-Pinene, （1r,4r）-（+）-campho, etc. 67 peaks were detected, and 41 components were identified from Alpinia Galanga, which is account for 78.29% of the total volatile components; the main substances were β-Sesquiphellandrene, Pentadecane, （E）-b-Farnesene, l-Caryophyllene. Composition and content of volatile components. The Composition and content of volatile components in Alpinia officinarum and Alpinia Galanga are very different, and they can not be mixed together.

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Determination of sweeteners and preservatives in seasoning tea-based drinks by high performance liquid chromatography

QU Hui-lin, DOU Kai-kai, QI Guo-ye, LIU Yun-feng, WANG Peng-tao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 139-141. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.031

Abstract （512）

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Develop a method for the determination of 2 kinds of sweeteners（saccharin sodium, aspartame） and 3 kinds of preservatives （benzoic acid, sorbic acid, dehydroacetic acid） in seasoning tea-based drinks by high performance liquid chromatography （HPLC）. A Diamonsil C₁₈ column （5 μm,250 mm×4.6 mm） was used with methanol -0.02 mol/L ammonium acetate （containing 0.4 mmol/L formic acid） solution as the mobile phase under the condition of gradient elution. Samples were extracted with water in an 50 ℃ ultrasonic bath for 30 min, and then they were analysed by VWD. Results show that the targets are baseline separated in 20 min. All correlation coef cients （r） are more than 0.999, with the average recoveries range from 83.0% to 108.0%, intra-day precision （RSD） values below 3.6% and inter-day precision （RSD） values below 4.4%. The method can be used for analyzing the targets in seasoning tea-based drinks.

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Analysis of volatile components of Citri Reticulatae Pericarpium viride by HS-SPME-GC-MS

HAN Man, JIANG Han-mei, MA Yin-yu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 142-144. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.032

Abstract （414）

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Using HS-SPME and GC-MS to extract and analyze the volatile constituents in Citri reticulatae pericarpium viride. The results showed that 68 peaks were detected from Mori folium, 56 components were identified, accounting for 87.98% of volatile components, including （+）-p-Mentha-1,8-diene（15.06%）, Linalool（10.88%）, cis-Carveol（7.88%）, trans-Carveol（6.49%）, α-Farnesene（6.26%）, α-Terpineol（3.59%）. HS-SPME-GC-MS combined technique was used to analyze the volatile components of Citri reticulatae pericarpium viride for the first time, providing reference for its further development and utilization.

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Determination of biogenic amines in aquatic products by a new pre-column derivatization RP-HPLC method

LI Su-yuan, YU Xi, HUANG Long, YU Jia

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 145-148. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.033

Abstract （411）

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A new pre-column derivatization and reversed high performance liquid chromatography （RP-HPLC） was used to detect 8 biogenic amines in 15 kinds of aquatic products.,8 biogenic amines could be separated by a new precolumn derivatization method. The results showed that in the range of 1.0 to 100 mg/L, 8 biogenic amines showed a good linear correlation（R²≥0.998）, the detection limit and quantitative limit of the method were lower than that of the national standard method. The average recovery rate of the added standard was 90%~110%. The method was simple to operate,derivatives are stable, few impurity, accurate and reliable results, can be used to detect biological amines in fish and dried fish products.

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Adventitious shoot induction and plant regeneration of lily varieties khaki

LI Hao, LIU Yu-chao, HUANG Zi-xuan, LI Jia

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 149-151. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.034

Abstract （490）

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With the bulbs of Lillium spp. var. Khaki as explants, MS medium plus different concentrations of plant growth regulators were used to inducing adventitious shoots and roots for plant regeneration. In this process, orthogonal experimental methods were used to analysis the impact of the concentrations and proportions of 6-BA, NAA, GA, IBA and active carbon to shoots and roots inducement of Lillium spp. var. Khaki. The results showed that the optimal medium for shoots inducement was MS+2.0 mg/L 6-BA+0.1 mg/L NAA+0.5 mg/L GA₃. After retesting, it was proved that the ratio of shoots inducement was 83.3% and the shoots were strong, fresh green and less browning. The optimal medium for rooting of adventitious shoots was 1/2MS+0.1 mg/L NAA+0.1 mg/L IBA and the ratio of rooting was 73.3%. The number of roots for each adventitious shoot was higher. The roots were strong and in good condition.

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Study on high efficient transformation of exogenous fatty acids into hydroxyfatty acids by engineering Escherichia coli

WANG Xiang-wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 152-155. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.035

Abstract （378）

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Cytochrome P450BM3 can hydroxylates fatty acids into hydroxyfatty acids（HFAs） at the ω-positions with high entioselectivity. Based on the construction of Escherichia coli carrying P450BM3 gene to transform fatty acids. As an effort to solve inhibit fatty acid β-oxidation and increase outer membrane fatty acids transporter, the lipoacyl-coa synthase gene（fadD） deletion and outer membrane proteins encode gene（fadL） overexpression strains were built. Under shake-flask conditions, this recombinant strains produced 748.8 mg/L hydroxy dodecanoic acid, meanwhile the conversion rate was 93.6%.

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Bioinformatics analysis of β-carotene converting enzyme （Asy） gene from phaffia rhodozyma

ZHANG Shuang, XU Yong, TONG Lin, ZHANG Zhong-dong, YE Hong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 156-158. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.036

Abstract （395）

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The amino acid sequence of the yeast extract of Astaxanthin was used as the research object. The physical and chemical properties and subcellular localization were determined by online prediction tools such as ProtParam, SignalP, PSORT, ProtScale, SOMPA, PredictProtein, PROSITE, COILS and SWISS-MODEL., pro-hydrophobicity, secondary structure, tertiary structure, etc. were analyzed. The invertase consisted of 557 amino acid residues and was a signal-free peptide, a transmembrane structure, and a soluble hydrophilic protein localized in cytochrome oxidase. The alpha helix and random coil are the primary secondary structural elements. The tertiary structure uses the B-chain of PDB ID 5vcc.1 as a template, and its function is predicted to be cytochrome P450. The bioinformatics method is more reliable in analyzing and predicting the results, which lays a theoretical foundation for further research on the biosynthesis regulation mechanism of astaxanthin.

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Study on coordinated development of population urbanization and land urbanization in Fuzhou city

JIN Ze-tao, LIU Ping-hui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 159-163. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.037

Abstract （462）

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Taking Fuzhou city as the research object, based on the four aspects of population structure, living standards, land structure, and land-use input-output, a coordinated development evaluation index system for population urbanization and land urbanization was constructed, and using the coordinated development model as the research method, the coordinated development relationship between population and land in Fuzhou from 2007 to 2016 was analyzed. The results show that the coordinated relationship between population urbanization and land urbanization in Fuzhou city has developed from serious disorder to a moderate one. The state of coordination between population and land urbanization has shifted from a lagging type of severely imbalanced land lagging type of primary coordination population lagging type. Fuzhou's population and land urbanization rate are relatively consistent, but the population urbanization rate is slightly insufficient.

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Impact of negative reports on trustworthiness of tourist destination： Analysis based on personality characteristics and perceived betrayal

YAN Zhi-wu, XIE Yun-hu, TIAN Qi, LI Jiang-min

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 164-169. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.038

Abstract （389）

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Based on the analysis of personality traits and perceived betrayal, a research model of "severity of negative reporting × personality traits - perceived betrayal - destination trust" was constructed and verified using the scenario test. The results show that for the same negative report, compared with the low severity group, the high severity group has a greater impact on destination trust; the psychological mechanism is that perceived betrayal partially mediates between the negative report and the destination trust. Personality traits have a moderating effect between negative reporting and perceived betrayal. Compared with rational personality, the perceived betrayal of potential tourists with emotional personality will be greater.

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Research on the development strategy of food tourism from the perspective of tourism industry cluster：A case study of Huanggang city Hubei province

CHENG Wen-qian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 170-174. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.039

Abstract （438）

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The location entropy theory was used to measure the tourism industry cluster related indexes of Huanggang city and its subordinate counties and cities in 2017. The results show that Huanggang's total tourism area entropy index is 1.25, showing a clear cluster trend; Macheng（1.62）, Hongan（2.53）, Luotian（3.03）, Yingshan（3.89） have formed an industrial agglomeration trend; and in Huangzhou（0.96）, Tuanfeng（0.85）, Qichun（0.82）, there has been a clear cluster trend. Based on the characteristics, development advantages and limiting factors of the food tourism resources in Huanggang city, the development strategies of food tourism in Huanggang city is analyzed from the perspective of industrial clusters.

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Study on the mechanism of recreational behavior in urban and rural areas based on SEM

LI Jiang-min, CHAI Ya-duo, GAO Jie

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 175-181. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.040

Abstract （484）

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Based on 1665 questionnaires for residents' recreation around the city of Wuhan, the structural equation model （SEM） was adopted to study the relationship between experience value, tourist satisfaction and behavioral intention, and to verify the "tourism experience value-satisfaction-behavioral intention" model （TEVSB） to reveal the mechanism of urban and rural recreation around metropolis. There are three links nested with each other： the first is the layered effect of the experience value; the second is the mediating effect of situational value and emotional value; the third is the direct effect of cognitive value on behavioral intentions. Functional value, economic value, situational value and emotional value have indirect effects on behavioral intentions through tourist satisfaction. Based on the conclusions, some suggestions are put forward to promote the development of rural-urban recreational areas.

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Research on the assistance of distressed children from the perspective of social governance——Taking the distressed children participating in the Spring Bud Plan in Shuyang county,Jiangsu province as an example

HE Meng-ting

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 182-186. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.041

Abstract （416）

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Children in distress are the most vulnerable groups in the entire group of children who need protection and support. The problems of left-behind children, seriously ill children, and unaccompanied children have caused widespread concern in society. In order to explore the problems existing in the current distressed children's rescue process, the suggestions for the choice of the distressed children's rescue route from the perspective of social governance are puts forward. By using the methods of literature research, case analysis and data analysis, the data of physical health, education level, family economic status of the 300 children in distress in 13 natural villages of Shuyang county, Jiangsu province,who participated in the Spring Bud Plan of Jiangsu Children's Welfare Foundation in 2017, were collected and analyzed. It was found that there were problems such as imperfect legal policies, simplification of the main body of assistance, low degree of specialization of the rescue work team, and imperfect rescue integration mechanism. From the perspective of social governance, a multi-disciplinary system based on government responsibility, non-government organization and professional welfare institutions （including children's welfare homes）, family care and community care should be constructed.

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The effects of land circulation and economic development on the ecosystem service value in Chongqing

ZHANG Wei-wei, ZHANG Li-ping, YANG Ye-hong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 187-193. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.042

Abstract （459）

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Using the land use change data of Chongqing in 1999—2008, using the equivalent factor table of China's terrestrial ecological service value, the change in ecological value caused by land transfer is estimated, and the impact of social and economic development on the value of ecological service is considered. The results show that the value of ecosystem services in Chongqing in 1999, 2004, and 2008 were 5,234,383,600, 5,493,479,600 and 5,525,143,700 yuan, respectively. The overall value of ecosystem services has shown a slowly increasing trend. The increase in the area of forest land and construction land and the decrease in cultivated land are the main reasons. In the two consecutive research sections, the value of cultivated land ecosystem services decreased the most, indicating that the development of construction land during urbanization has seriously affected the ecosystem service functions of Chongqing. In addition, from 1999 to 2008, the proportion of Chongqing's GDP to the value of ecosystem services is getting smaller and smaller, indicating that rapid economic growth is negatively related to the value of ecosystem services. The research results can provide reference suggestions for the government in making optimal land allocation and transfer decisions to ensure coordinated economic, social, and ecological development.

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Impact of environmental protection tax collection on enterprises and its planning strategies

HU Jing-jun, LIU Xi-yu, SHEN Xiao-mei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 194-197. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.043

Abstract （504）

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In the face of increasingly serious environmental problems, China officially levied environmental protection taxes in January 2018. The collection of environmental protection tax echoes the urgent need of China's environmental protection cause, and it will inevitably affect the behavior choices of residents, enterprises and the whole society. First of all, from the three stages of development, basic formation and development and improvement, we will sort out the evolution process of China's environmental protection tax, and clarify the similarities and differences between environmental protection tax and pollution charge. Secondly, the opportunities and challenges of environmental protection tax collection are discussed for environmental protection enterprises and non-environmental protection enterprises. Finally, the tax planning strategies of enterprises are discussed from the aspects of tax reduction and taxation technology, tax rate difference technology and deduction technology.

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Building of the functional zones of grain production in the Mufu mountain area,Hubei province

SUN Zhi-guo, LIU Zhi-yang, XIONG Wan-zhen, ZHONG Ru-gang, DING Guang-ping

HUBEI AGRICULTURAL SCIENCES 2020, 59 (1): 198-201. DOI: 10.14088/j.cnki.issn0439-8114.2020.01.044

Abstract （418）

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The establishment of functional areas for grain production is an important measure to ensure the national grain security and optimize the distribution of agricultural production. It will help to further promote the structural reform of agricultural supply side, and comprehensively implement the strategy of storing grain for the land and storing grain for technology.The Mufu mountain area, is one of the four main battlegrounds of targeted poverty alleviation in Hubei province. The overview of building the functional zones of grain production in China and Hubei province was introduced, the functional zones of grain production in the poverty alleviation area was studied, the tactics to build functional zones of grain production under the rural revitalization strategy and the targeted poverty alleviation strategy were explored.

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Advances in extraction,purification,chemical modification and antioxidant activity of polysaccharides

MENG Shu-yu, MERWERT·Adalhan, XIAYIDA·Nurmamate, ADELE·Suleiman, MULLIDER·Hederbai, CHEN Chun-li

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 5-8. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.001

Abstract （516）

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Polysaccharide has become a hot research topic in recent years because of its high activity, low toxicity, natural, wide source and so on. The extraction, purification, chemical structural modification and antioxidant activity of polysaccharide are reviewed.

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Biological function and application of new plant growth regulator strigolactones

ZHENG Chen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 9-13. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.002

Abstract （970）

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Strigolactones （SLs） is a general term of the alcohol compounds of striga. It is a kind of signal molecule which can regulate the endogenic development of plants in recent years. It is called a new plant hormone. Recently, much researches have been done on SLs, there are many reports on the study of striga compounds and their functions. The research and crop cultivation application of the biological functions of SLs were reviewed,and the future research of SLs was also forecasted.

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Research advances on plant effects of engineered carbon nanomaterials

ZHANG Guan-lin, GAO Cun-bin, LIU Yu-zhen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 14-19. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.003

Abstract （557）

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With the development of nanotechnology, engineered carbon nanomaterials are widely used in many fields. Meanwhile, ECNMs inevitably release into the environment, which will enhance the chances of contact with organisms. Biological toxicity and potential environmental risks can not be ignored. Plants are an important source of human food. At present, the researches have shown that ECNMs have toxic effects on plants and threaten food safety and human health. Based on the brief introduction of the environmental sources of ECNMs, the research progress of the effect and mechanism of ECNMs on the physiological growth of plants at home and abroad was summarized, the current research methods and challenges were discussed, so as to provide theoretical basis and practical reference for the biosafety assessment, plant protection and rational planning and using of raw materials of ECNMs.

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Effects of nitrogen fertilizer and sowing methods on yield and nitrogen use efficiency of Xinmai No.9

XIE Xu-dong, ZHOU Guo-qin, CHEN Zhen-zhen, XU Hong, ZHANG Yu-bo, ZHANG Bo, LI Gang, LI Yu-feng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 20-23. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.004

Abstract （563）

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From 2014 to 2015, the research on the yield and nitrogen use efficiency of Xinmai No.9 was carried out in Xinyang City,Henan Province. Three nitrogen fertilizer levels of 0, 150 and 225 kg/hm² were set, and two sowing methods of broadcast and drill seeding were set. The yield,dry matter accumulation and grain nitrogen content of Xinmai No.9 increased with the increase of nitrogen application. The highest yield were achieved when the nitrogen fertilizer level was 225 kg/hm² in the two sowing methods. With the increase of nitrogen supply, the nitrogen uptake of the plant above ground and the amount of grain nitrogen accumulation increased, but the nitrogen uptake efficiency, nitrogen utilization efficiency, nitrogen agricultural use efficiency, nitrogen partial factor productivity, and the apparent nitrogen recovery efficiency were all declined, and the nitrogen physiological efficiency increased. Under the current production level of wheat after rice in south Henan province, 225 kg/hm² of nitrogen application can obtain higher yield and economic benefits under the treatment of strip sowing.

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Correlation analysis of essential amino acids,total amino acids and protein contents of different rice varieties

ZHANG Shun, LI Zhi-jian, ZHANG Yue-fei, YOU Yong, ZHANG Zhi-yuan, TANG Jian-zhou

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 24-25. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.005

Abstract （540）

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The essential amino acids, total amino acids and protein contents of 28 refined white rice samples were determined, and the correlation between the components of different refined white rice samples was analyzed. The results showed that the correlation coefficients of protein content and total amino acid content, protein content and essential amino acid content, total amino acid content and essential amino acid content of 28 samples were 0.940, 0.950 and 0.995, respectively. There was a very significant positive correlation between these indexes. The nutritional value of protein, the main nutrient component of white rice, mainly lies in the content of amino acids. The content of essential amino acids can be used as an important index to judge the nutrition of rice

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Analysis on the characteristics of the yield structure of the approved japonica rice varieties in Henan province in 2009—2018

WANG Ling-yan, HUANG Jin-hua, WANG Shu-yu, ZHANG Su-ping, MA Hai-tao, LIU Zhen-yu, XIA Yan-li, DOU Shi-shu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 26-29. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.006

Abstract （499）

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Using 36 japonica rice varieties approved in Henan province from 2009 to 2018, correlation analysis, partial correlation analysis and path analysis of their eight traits and yield were conducted to study the internal relationship and evolution of main agronomic traits affecting japonica rice. The results demonstrated that the average yield of japonica rice showed an upward trend, with an annual increase of 129.11 kg/hm², and the growth period showed a downward trend, which was 0.826 day per year, other characteristics didn’t change significantly. The eight traits have different effects on the yield of japonica rice. The number of grains per panicle is the main trait that affects the yield. The number of effective panicles, 1 000-grain weight and plant height are also important factors to yield. Therefore, under the premise of a reasonable and effective panicles, the main goal is to increase the number of grains and ensure the 1 000-grain weight.

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Dynamic transformation of carbon in agricultural waste-compost and its fertilizer efficiency in citrus planting

LIAO Lei, XIONG Feng, ZHOU Yue-ming, LEI Yang-ming

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 30-36. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.007

Abstract （474）

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The dynamic composition and quantity of organic carbon in composting were analyzed by Van Soest analysis method, its influence mechanism on product quality during composting was also analyzed combined with procedure parameter. The results showed that, the duration of the high temperature period was 36 days, pH was 8.11, moisture content was 25.17%, OM was 52.34%, TN was 5.41%, effective number of viable bacteria was 9.6×10⁸ CFU/g, value of escherichia coli was 35 CFU/g, mortality of ascarid egg was 98%, germination index was 93.09%, which satisfied the requirements of NY 8884-2012 for biological organic fertilizer. The insoluble components of LIG, HEM and CEL in carbon composition were significantly correlated with total nutrients, OM, C/N（correlation coefficient was all greater than 0.875）. The degradation of insoluble macromolecular substances into small molecular substances which easily used by microorganisms is the key factors to improve the quality of fertilizer, whereas the loss of TOC will significantly reduce the quality of fertilizer. The application of the organic fertilizer in citrus plantations showed that the organic fertilizer could improve the quality and yield of citrus. The best quality and maximum yield of citrus were got with the application amount of 2.0 kg/m².

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Preliminary report on application effect of CM biological nitrogen fixation fertilizers on rice

ZENG Yan-ling, LUO Zhi-yong, FENG Chun-shui, LI Mei-ling

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 37-39. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.008

Abstract （637）

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The effects of CM biological nitrogen fixation fertilizers on seedling quality, agronomic traits and yields were studied, and its application was explored in rice production. The results showed CM biological nitrogen fixation fertilizers improved the quality of seedlings and agronomic traits, which had obvious effects of reducing nitrogen, increasing yield and increasing income.

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Influence analysis of meteorological factors on oleic acid of Camellia oleifera and model construction

PENG Jia-dong, LIAO Yu-fang, JIANG Yuan-hua, HUANG Chao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 40-43. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.009

Abstract （477）

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Based on the collection and processing of the oleic acid data from multiple Camellia oleifera sample plots in different county of Hunan province during 2012—2017 and the meteorological indices for each stage of growth and treatment of Camellia oleifera which computed by the nearby meteorological observation stations’ data during the same period, the method of principal component analysis had been used to research the impacts of the meteorological factors in different stages on content of oleic acid for Camellia oleifera, and a forecasting model were constructed based on above meteorological factors. The results showed that the effects of meteorological factors for the whole year of fruit picking, oil transformation and accumulation peak period, fruit enlargement peak period on oleic acid content were more significant than other periods, and the precipitation indexes, especially the precipitation and the longest continuous precipitation days were the key factors which affecting oleic acid. The oleic acid forecasting model constructed base on those factors had a relative high accuracy and had certain applicability.

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Analysis on climatic characteristics and influencing factors of hail days in Hunan province from 1979 to 2018

SANG You-wei, MENG Lei, HE Na, HUANG Wu-ying

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 44-48. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.010

Abstract （476）

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Hail days observation data from 95 weather stations in Hunan province and NECP 0.5°×0.5° reanalysis data from 1979 to 2018 was used to analyze the climatic characteristics and influencing factors of hail days in Hunan by linear trend estimation, M-K test, wavelet analysis, and similarity analysis. The results showed that the season of hail in Hunan province was obvious, the months from January to May are the main hail months, and the number of hail days in March was the most, which was a typical spring hail area; In general, hail in Hunan province was more in the northwest and less in the southeast, decreasing from northwest to southeast; In recent 40 years, the number of hail days in Hunan province had decreased significantly, and the decrease trend was the most significant in March, at the same time, the number of hail days presents periodic changes, especially in cycles 2~3 years and 4~6 years, 3 years cycle fluctuation had been the main trend since 2000; The decrease of hail days in Hunan province mainly came from the significant decrease of hail days in the areas with high incidence of hail, most stations in northwestern Hunan, northern Hunan, southern Hunan and central Hunan had a significant decrease of hail days; The spatial distribution and variation trend of hail in Hunan province were significantly correlated with the distribution and variation trend of atmospheric stability K index, in the regions with monthly average K index lower than 23, the high value area of hail corresponds to the relatively high value area of K index at the same latitude, and the regions with significantly decreased number of hail days correspond to the regions with significantly increased K index.

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Distribution and risk evaluation of hail disaster of stagger-season strawberry production area in Sichuan province

ZOU Yu-jia, ZHANG Yu-fang, DAI Xin-lu, GUO Xiao-mei, HUANG Long-fei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 49-54. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.011

Abstract （396）

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Based on the hail disaster data from 1980 to 2010, the temporal and spatial distribution of hail disaster in stagger-season strawberry production area of Sichuan province was analyzed. Combined with the geographic information data and hailstorm operation station data, the hailstorm disaster risk assessment model was constructed from three aspects of hazard risk, hazard bearing sensitivity and disaster prevention and reduction ability, and the hailstorm risk map was completed based on GIS. The results showed that the hail occurred every year in the stagger-season strawberry production area in Sichuan province, mostly from March to October, and the hail frequency showed a decreasing trend in 30 years. The number of hail days was the most in Ganzi prefecture and the least in Panzhihua-Xichang region. The risk of hail disaster gradually decreases from northwest to southeast, and the high risk areas are concentrated in the northwest and central parts of the northwest Sichuan plateau, including Shiqu county, Seda county and Litang county, while the low risk areas are mostly concentrated in Panzhihua-Xichang region.

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Early warning of cultivated land quality in Henan province

SONG Yan-hua, WANG Ling-chao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 55-61. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.012

Abstract （405）

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The cultivated land productivity early warning system is established to provide critical warning for regional cultivated land quality and provide early warning for the decline of cultivated land productivity. The negative number principle method is used to divide the warning threshold. Various models are established to calculate the change of cultivated land productivity caused by the different quantity of supplementary cultivated land and occupation cultivated land, the change of cultivated land productivity caused by the different quality of supplementary cultivated land and occupation cultivated land, the change of cultivated land productivity caused by the Land remediation. The results show that the change of cultivated land quantity has different influence on the quality and productivity of cultivated land. Affected by the different quality of supplementary cultivated land and occupation cultivated land, the relationship between cultivated land quantity and quality is non-positive but the relationship between cultivated land quantity and productivity is positively. Affected by different causes of cultivated land quality change, the change of cultivated land quality and productivity is not proportional to each other. Cultivated land productivity can better reflect the health status of regional cultivated land protection. The construction of cultivated land quality can improve the quality and productivity of cultivated land when the quantity cannot be balanced. The analysis of the early warning results and causes of cultivated land can provide more targeted measures for regional cultivated land protection. It is concluded that cultivated land productivity can be used as an early warning index of cultivated land quality. Early warning of cultivated land quality can avoid the serious deterioration of cultivated land quality. The early warning results can provide real-time services for the continuous utilization of land resources. The results have certain foresight to cultivated land grain production. The results are helpful to ensure the stability and safety of food production under sustainable development.

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Purification evaluation and management of fish pond water quality by ecological floating bed：Taking the fish pond of Huayu Garden Nursery stock ecological garden as an example

LAI Xin, FU Jin, ZHANG Xi-zhi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 62-66. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.013

Abstract （415）

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Taking the fish pond of Huayu Garden Ecological Nursery as the research object, total nitrogen （TN）, total phosphorus （TP）, ammonia nitrogen and chemical demand （COD） in water before and after planting ecological floating bed as evaluation indexes, the water quality was tested. Respectively from two dimensions of time and space,the absorption effect of ecological floating bed on nitrogen and phosphorus in water and the influence trend of of COD in water were analyzed and compared. The results showed that the ecological floating bed for the whole water of nitrogen and phosphorus and COD has certain absorption effect, including removal rate up to 15.2% of total nitrogen, total phosphorus removal rate up to 56.3%. The management concept of ecological floating bed for fish pond water purification was put forward.

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Research on the management of action mechanism between population spatial factors and green development space

JIANG Yu-xiao, ZHU Ming-cang, GU Ji-rong, ZHAO Yuan-di

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 67-73. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.014

Abstract （522）

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Under the concept of inclusive economic growth with the theme of green development, research the feedback mechanism between the population spatial factors and green development space. By using geographic information method and spatial analysis technology, and based on the monitoring results of geographical conditions, the spatial elements of green development were extracted to form the spatial pattern of green development. At the same time, the spatial carriers of social and economic factors were extracted, the statistical indicators of social economy were quantified, and the traditional resource factors and innovation-driven factors in social economy were measured. Using the multiple logistic regression model to construct the feedback equation between population spatial factors and green development space, to establish the feedback mechanism between them. It can be found from the study of Chengdu that the growth of innovative population improves the efficiency and potential of land use, leading to the decrease of land demand, and the population density in the same space unit can be increased by up to 49%. The increase of innovative population has a positive incentive effect on green development, while the increase of traditional population has a negative incentive effect on green development.

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Gini coefficient of ecological security of water resources in 7 districts of Shangluo city

LIU Yan, LI Xuan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 74-78. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.015

Abstract （469）

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Based on the basic principles of the Lorenz curve and the Gini coefficient calculation method, the degree of matching between water resources and cultivated land resources, GDP, population and grain yield makes in Shangluo city were studied. The Gini coefficient corresponding to the water resources and cultivated land area, GDP, population and grain yield in Shangluo city was 0.201 4, 0.174 6, 0.251 0 and 0.236 0, respectively. The analysis showed that the match of the water resources and cultivated land resources, GDP, population and grain yield were good, concluding the overall level of ecological Shangluo city was good. And the corresponding countermeasures and suggestions for the future development of regional economy in Shangluo city was put forward.

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Experimental comparison of germplasm resources different varieties of Astragalus sinicus

FAN Mei-rong, ZHANG Chun-xia, LIAO Yu-lin, XIE Bai-cheng, LU Yan-hong, NIE Jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 79-81. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.016

Abstract （427）

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The growth period, characteristics, yield of fresh plant, nutrient content of four varieties of Astragalus sinicus on pot-experiment were compared and analyzed. The results showed that Xiangzi No 3 and Xinyang Astragalus sinicus were more suitable to plant in double-cropping rice area than other varieties. The plant characters of Xiangzi No 3 such as leaf color, plant height, branch number, true leaf number, root length, number of rhizobia before winter were all better than the other varieties. There was no significant difference in stem diameter, number of branches and weight per plant among Yujiang large leaves, Yuanjiang specie and Xiangzi No 3 at the full-bloom stage, and these indexes were significantly higher compared with Xinyang Astragalus sinicust. The fresh grass yields of the four varieties were manifested included： Yujiang Large Leaves >Yuanjiang Species>Xiangzi No 3>Xinyang Astragalus sinicust at the full-bloom stage. Xiangzi No 3 had the highest total nitrogen and total potassium content among the four varieties. There was no significant difference in total phosphorus content among the four varieties.

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Identification and biological characteristics of the pathogen from peanut web blotch in Liaoning province

XIE Jin-hui, LIN Ying, ZANG Chao-qun, PEI Xue, LIANG Chun-hao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 82-86. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.017

Abstract （427）

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In order to confirm the pathogen of peanut web blotch in Liaoning province, the pathogenicity of pure cultures separated from the disease samples was determined by Kirsch's rule, and the pathogenic bacteria were identified according to their morphological characteristics and ITS sequence analysis. The biological characteristics of pathogenic bacteria were studied by colony growth method and concave glass slide germination method. The results showed that the pathogen of peanut netted spot disease in Liaoning province was Peyronellaea arachidicola. The optimum temperature of mycelium growth was 23 ℃. The best carbon source was maltose, the best nitrogen source was yeast paste, the optimum pH was 7, the light dark alternation was beneficial to the mycelium growth and the mycelial lethal temperature was 45 ℃. The optimum temperature for conidia germination was 25 ℃, the optimum pH was 7, and the illumination condition was beneficial to conidial germination.

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Evaluation of the control effect of biocontrol bacteria Bacillus amyloliquefaciens D2WM on Dickeya chrysanthemi and its fungicides screening

CHEN Jia-min, LIU Hui-ying, ZHANG Xiang-hui, DENG Ting-ting, WEI Mi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 87-92. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.018

Abstract （480）

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Single factor test was used to optimize the extraction efficiency of Bacillus amyloliquefaciens D2WM antimicrobial substance, and the storage characteristics of crude extraction of antimicrobial substance were studied. The stability of crude extracts and the fungicides screening on Dickeya chrysanthemi were studied. The results of biocontrol agents and commercial agents were compared. The results indicated that the optimum extraction solvent was n-butyl alcohol, the volume ratio of organic solvent to fermentation broth was 1∶7, and the extracting time was 0.5 h. In addition, the antimicrobial substances showed excellent stability towards temperature and ultraviolet. The antimicrobial activity was not influenced when exposed at 100 ℃, or after treatment of ultraviolet irradiation for 12 hours, the size of the antimicrobial circle was still 23 mm. When the antimicrobial substances were stored for 180 days, the size of the antimicrobial circle was still 16.33 mm. The crude extracts of biocontrol bacteria Bacillus amyloliquefaciens D2WM had good bacteriostatic effects on plant pathogens such as Pectobacterium atrosepticum, Pectobacterium carotovorum, Dickeya chrysanthemi, Pseudomonas syringae and Xanthomonas arboricola. Only New-tian Xiucai No. 6, Zhongshengmycin, benziothiazolinone and chloroisobromine cyanuric acid had good inhibitory effects on Dickeya chrysanthemi among the 19 tested medicines. It was found that the bacteriostatic effect of crude extract of biocontrol bacteria Bacillus amyloliquefaciens D2WM was higher than benziothiazolinone and chloroisobromine cyanuric acid.

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Effects of tolfenpyrad on toxicity and behavior of Solenopsis invicta worker ants

HUANG Shi-you, CHEN Hui-ya, MA Qian-li, WANG Yong-qing, ZHANG Zhi-xiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 93-97. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.019

Abstract （481）

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The effects of tolfenpyrad on the behavior of worker ants of Solenopsis invicta Buren were studied by using water tube to feed poison method. After 10 h treatment with 5, 10 and 20 mg/L tolfenpyrad, respectively, the mortality rate of the small worker ants was 58.3%, 97.5% and 83.3%, much higher than that of the control group with only 10% of the mortality rate. The medium mortality rate of red fire ants was 51.7%, 86.7% and 60.0%, respectively, which was significantly higher than that of the control group with 3.3%. After 10 h treatment of 0, 5, 10 and 20 mg/L, the walking rates of small red imported fire ants was 93.3%, 31.7%, 0, 3.3%, the grasping rate was 93.3%, 21.7%, 0, 3.3%, and the climbing rate was 93.3%, 21.7%, 0, 3.3%, respectively. The walking rate of red fire ants was 96.7%, 63.3%, 8.3% and 31.7%, the grasping rate was 96.7%, 46.7%, 5.0% and 20.0%, and the climbing rate was 96.7%, 55.0%, 5.0% and 26.7%, respectively.

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Study on the identification of Magnoliaceae plants based on the alcohol-soluble protein

YE Shan-shan, WANG Nan, GAO Yan-fang, TANG Mi, LIU Meng-ling, XIAO Qiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 98-101. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.020

Abstract （429）

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The Magnoliaceae plants are widely distributed and varied in genus and species. There are many controversies in the identification of genus and species because many transitional types were found among different genus and species. At present, botanical shape characteristics are usually used as identification methods for the classification and identification of magnoliaceae plants. In order to further identify the magnolia plants, the acid polyacrylamide gel electrophoresis was used to analyze the gliadin of magnolia plants. The results showed that 61 bands were separated from 10 species of magnolia,and most of the bands were between 6 and 7, with a maximum of 9, and the lowest 6. Using Quantityone software to analyze electrophoresis bands, it is found that there are differences between different bands, and species identification can be carried out through spectral bands.

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Research on the detaining dust plant community structure in urban road

CHEN Ying-jia, LIU Zhong-bing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 102-105. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.021

Abstract （435）

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The study on the community structure of urban road plants was reviewed and analyzed, and the connotation and construction of the structure of the plant community in the urban road were put forward. The stability and variability of plant community structure, the combination of detaining dust function and multi-function, the construction and development of detaining dust plant community structure and green space system were discussed.

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Effects of different light quality on the coloration of the leaves of the Crassula capitella‘Campfire’ of crassulaceae

LIU Mei-zhou, LI Yi-zhu, ZHANG Rui, GE Jia-rong, REN Yi, ZHANG Xue, LIU Xiao-jia, QI Yi-jie

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 106-109. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.022

Abstract （354）

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Taking the leaf cutting seedlings of annual Crassula capitella ‘Campfire’ as materials, the effects of light quality on color difference, chlorophyll and anthocyanin content of annual Crassula capitella ‘Campfire’ leaves were studied after three months of treatments with white light,red light,blue light and ultraviolet, then the effects of light quality on the coloration of the Crassula capitella ‘Campfire’ leaves were analyzed. The results showed that different light quality was important in the coloration process of Crassula capitella ‘Campfire’ leaves. Among them,ultraviolet had the greatest effect on the color difference and red saturation of Crassula capitella ‘Campfire’ leaves, and effectively increased the content of carotenoids and anthocyanins in leaves, and had the best effect on promoting the color of Crassula capitella ‘Campfire’ leaves.

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Changes of endogenous growth regulators during roots of tissue culture seedlings of Lonicera macranthoides Hand.-Mazz

CAO Zheng-yan, TANG Ning, CHEN Ze-xiong, LOU Juan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 110-113. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.023

Abstract （439）

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The tissue culture seedlings of Lonicera macranthoides Hand.-Mazz were used as experimental materials,the changes of the content of indole-3-acetic acid （IAA）, abscisic acid （ABA）, gibberellic acid （GA₃）, and zeatin nucleoside （ZR） in the root-inducing process （0,6,12,18,24,30 d） were determined by the method of enzyme-linked immunosorbent assay（ELISA）. The results showed that the content of ABA was increased initially and then decreased. The concentrations of IAA, GA₃ and ZR displayed the opposite trends, which were decreased firstly and subsequently increased. The contents of IAA and GA₃ and the ratio of IAA to ABA were significant or extremely significant positively correlated with rooting rates, while ABA contents were negatively correlated with rooting rates, and there was no significant correlation between ZR content and rooting rate.

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Analysis of the correlation between the soaking time and the emergence rate of the seed of Ricinus communis L.

JIN Fang-lun, LUO Chao-bin, LUO Hui-xian, WU Kang-yun, WANG Xian-yu, JIN Ling

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 114-119. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.024

Abstract （512）

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The correlation between the soaking time and the emergence rate of the seed of Ricinus communis L. was investigated and studied. Results showed that there was a significant negative correlation between the different soaking time of Ricinus communis L. seeds and the total number and rate of emergence of seeds, but no significant correlation with other indexes. The quadratic curve regression equation and linear regression equation were used to analyze the different soaking time, the total number of seedlings, the total rate of emergence and the rate of no nest of Ricinus communis L. seeds. The results showed that the regression equation of the different soaking time and the total number of seedlings, the total rate of emergence and the rate of no nest of Ricinus communis L. seeds were all in accordance with the quadratic curve regression equation.

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Geographical indication characteristics and unique production technology of rice in Huanghekou

LIU Yan-gang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 120-122. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.025

Abstract （426）

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It was recognized as a national agricultural product geographical indication product in 2011. The geographical indication characteristics of rice in Huanghekou were expounded, including specific production area, unique production environment, human history and unique product quality, the unique production mode of rice in Huanghekou was summarized from the aspects of production area selection, variety selection, production management process, harvest and processing, aiming to maintain the brand quality of rice in Huanghekou to the greatest extent and further improve the quality of rice in Huanghekou brand awareness and market competitiveness of Huanghekou rice in the national and even international market.

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New cultivars of Lagerstroemia indica Lagerstroemia indica ‘Danzhao Zihuan’ and Lagerstroemia indica ‘Honghuan Danzi’

WANG Ye-she, CHEN Li-jun, YANG Xian-jun, DUAN Lin-dong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 123-124. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.026

Abstract （483）

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The new varieties of Lagerstroemia indica Lagerstroemia indica ‘Danzhao Zihuan’ and Lagerstroemia indica ‘Honghuan Danzi’ were selected through collecting widely seeds of various sources and varieties of crape myrtle, developing of mixed seed grow seedlings and using natural hybridization seedling-selection method. After cultivation experiments, the two new varieties are fine garden flower tree species because they have the characteristics of stable genetic character, wide adaptability, strong stress resistance and high ornamental value, and has been applied in city landscaping.

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Study on the main flavor amino acids of the hemp chickens in the free-range breeding mode in northern mountains of Guangdong：Take Qingyuan pai chicken as an example

CHENG Tian-de, ZHONG Nan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 125-127. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.027

Abstract （556）

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The amino acids of hydrolysate of raw chicken and cooked chicken of Qingyuan pai chicken in the free-range breeding mode have been determined and analyzed by automatic analysis of amino acid. The results showed that the species of free amino acid were almost the same between the hydrolysate of raw chicken and the cooked chicken, but the content of amino acid in hydrolysate of raw chicken was significantly higher than cooked chicken, the content of those amino acids which taste bitter in hydrolysate of raw chicken（Arg, Lys, His, Met and Val） was higher and the TAV was bigger, the main umami amino acid （Glu） in proportion is obviously improve in cooked chicken than in the raw chicken, the main sweet amino acids in raw chicken hydrolysate and cooked chicken （chicken soup） were Ala, but the content and TAV were lower.

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Comparative study of three kinds of rhubarb processed products on the draining effect of Dachengqi decoction in mice

QIU Mei-yan, WEI Jiang-cun, ZHANG Ru, XIE Zhen, JIANG Xian-zai, HAN Qian, ZHAO Feng-xian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 128-132. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.028

Abstract （407）

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To study the difference of the purging effect of rhubarb, vinegar rhubarb and rhubarb charcoal on Dachengqi decoction, 96 experimental mice were randomly divided into positive control group, blank control group, DCQ 6,10 g/kg dose group, vinegar DCQ 6,10 g/kg dose group and carbon DCQ 6,10 g/kg dose group. The activities of rhubarb, rhubarb and Dachengqi decoction of rhubarb and rhubarb on normal diarrhea, propelling rate of carbon and Na⁺-K⁺-ATPasc activity in mice were compared. Results showed that the effects of rhubarb, rhubarb and rhubarb charcoal on the diarrhea of Dachengqi decoction were different, but the variation of Dachengqi decoction was related to rhubarb, rhubarb and rhubarb carbon. Compared with the blank control group and the positive control group, the DCQ group, the vinegar DCQ group and the carbon DCQ group had different effects on normal diarrhea, the propulsive effect of carbon end and the inhibition of the activity of Na⁺-K⁺-ATPase in normal mice, and the difference of the effect between the DCQ group and the DCQ group was the most significant. The effects of rhubarb, rhubarb and rhubarb carbon on the diarrhea of Dachengqi may be different, which may be related to the changes in the content of the main chemical composition of the radix dahuris, rhubarb and rhubarb charcoal.

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Study on storage application of carton packing sheet smoke without lining bag

SU Zan, SUN Jian-sheng, LI Ji-gang, TANG Yu-xin, HU Ya-jie

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 133-136. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.029

Abstract （364）

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In order to verify the applicability of the storage technology of carton packing sheet tobacco non-lined plastic bag in large-scale storage of tobacco raw material warehouse, the appearance quality, chemical composition and sensory quality were measured by selecting six grades of tobacco leaves in four production areas and adopting the comparison storage of non-lined bags and lining bags. The results showed that after 18 months of storage, compared with the tobacco with inner lining bag, the smoke without inner lining bag had a lighter color change, better chemical composition and better sensory quality. It provided the basis for improving the curing quality and optimizing the store storage technology.

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Effect of different storage temperature on quality and weight loss rate of sweet potato

YAN Hai-feng, LI Wei-liu, XIONG Jun, WEI Ming-zhen, TANG Xiu-hua

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 137-140. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.030

Abstract （416）

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To examine the effect of storage temperature on quality and loss rate of sweet potato for its suitable storage, Guijingshu 8 was stored at room temperature and at temperature of （9±0.5） ℃ with humidity of （85±5）%,respectively, and weight loss rate, dry matter content, total soluble sugar, starch content, protein and vitamin C content were determined after 0,15,30,45,60 and 75 days storage, respectively. The results showed that compared with the room temperature storage, the weight loss rate reduced by 7.1 percent, the dry matter content reduced by 3.06 percent, and the total soluble sugar increased with 51.06% in 9 ℃ after 75 days storage. The content of starch and protein was not significantly different between the room temperature and 9 ℃ after 75 days storage. The content of vitamin C reduced by 56.21% in room temperature, but only reduced by 41.62% in 9 ℃ after 75 days storage, the content variation exhibited significantly difference between the two storage conditions at this time. But the variation of the vitamin C content between room temperature and 9 ℃ storage was not significantly different after 75 days storage. In conclusion, 9 ℃ was beneficial for Guijingshu 8 storage.

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Extraction technology of anti-inflammatory constituent asperuloside from Psychotria serpens L.

HONG Ying-wen, LIANG Zi-ning, LIAO Gui-xiu, WEI Li-cui, LONG Qi-yun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 141-144. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.031

Abstract （440）

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HPLC was used to establish methods of content determi-nation of asperuloside in Psychotria serpens L.. Asperuloside as index components,on the basis of methanol concentration,liquid ratio and extraction time,we carried out experiments of single factor and orthogonal design to further optimize the extraction process. Optimum extraction of Glycyrrhizic flavonoids：70% methanol,solid-liquid ratio 1∶60,extraction time 30 min.

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Process optimization of chlorogenic acid in honeysuckle of Shangluo by ultrasound-assisted alcohol extraction

HE Jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 145-148. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.032

Abstract （358）

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Aimed at probing a suitable extraction process for local production with the the honeysuckle of Shangluo city. Based on the alcohol extraction method, ultrasound-assisted extraction was carried out with the orthogonal test results on the basis of single factor test. The optimal extraction process of chlorogenic acid in honeysuckle of Shangluo were as followed ethanol mass fraction was 50%, ultrasonic time was 40 min, ultrasonic temperature was 65 ℃, solid-liquid ratio was 1∶40, the extraction rate of chlorogenic acid could be 4.305%.

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Analysis of volatile components extracted from Magnoliae flos and mixed product by HS-SPME-GC-MS

HAN Man, JIANG Han-mei, LU Jin-qing, DAI Yu-xin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 149-152. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.033

Abstract （399）

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The volatile components extracted from Magnoliae flos（dried flower buds of Magnolia biondii Pamp.） and mixed product （dried flower buds of Magnolia liliflora Desr.） were analyzed by HS-SPME-GC-MS. Results showed that 30 and 41 compounds were separated and identified from Magnoliae flos and mixed product respectively,there were 12 common components, the highest content of the volatile components in Magnoliae flos is g-Terpinene（26.68%）, and the highest content of the volatile components in mixed product is galcanfor （35.67%）.

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Design on cut twist micro-plough

SUN Peng, MAO Ting-ting, QIU Wan-li, WANG Yuan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 153-156. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.034

Abstract （319）

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In order to solve the problem that the micro tillage machine was easily entangled by weeds, low efficiency and high labor intensity during the cultivation of Yunnan mountain, a new type of micro tillage machine was designed, which was simple in structure, small in size and easy to operate. Through field experiments, it was concluded that this machine can not only guarantee the stability of soil tillage, but also improve the soil breaking rate and achieve the desired effect.

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Rice planting area information extraction based on multi-temporal remote sensing data

PENG Le-wen, ZHANG Ya

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 157-160. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.035

Abstract （452）

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In remote sensing images, the moisture content and soil moisture of plants were sensitive to the short-wave infrared band, while the red band and near-infrared band reflected the coverage rate and growth rate of plants strongly. According to the characteristics of rice growing in moist soil, based on a decision tree rice extraction model with time difference, this paper carried out relevant research on rice growing area extraction in sheyang county, yancheng city, jiangsu province by calculating NDVI and LSWI of normalized vegetation index and soil moisture index at different growth periods. The comparison of the extracted rice area and local statistical data showed that the model can effectively distinguish the water area, corn and vegetable fields that were easily confused with rice, and the precision of area extraction reached 76.26%.

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Estimation model of precipitable water vapor based on deep LSTM neural network

LUO Yu, LUO Lin-yan, FAN Jia-zhi, DUAN Si-ru, GAO Wen-juan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 161-165. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.036

Abstract （509）

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Based on deep long short term memory（LSTM） neural network, the estimation models （LSTM₅ and LSTM₁） of precipitable water vapor （PWV） in Huaihua distinct are proposed by using multi（air temperature, air pressure, dew point temperature, relative humidity, water vapor pressure and hourly precipitation） and single factor（water vapor pressure） separately, besides the estimation precision of the models are analyzed. As the result shown, the LSTM models have high estimation precision, whose coefficients of determination are both greater than 0.94, mean values of root square error, mean absolute error and mean absolute percentage error are below 1.158 1 mm, 0.709 9 mm and 4.54% respectively. The precision of LSTM models improves more than 70% compared to linear estimation model or quadratic polynomial estimation based on water vapor pressure, and the precision of LSTM₁ model slightly better than that of LSTM₅. The distribution of estimation errors relates to PWV value, which is first increased and then decreased along with the increasing of PWV. Furthermore, there is negative correlation between the estimation precision of the models and altitude of the stations.

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The Cep164 gene indirectly regulate CP110 expression in the centriole

WANG Chun-hua

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 166-168. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.037

Abstract （385）

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Cep164 is one of the important genes involved in ciliogenesis. In order to study the relationship of the Cep164 and CP110, explored the role of CP110 in the regulation of ciliogenesis by Cep164. Immunofluorescence staining and Western Blotting were used to determine the expression of CP110 gene in the Cep164 gene knockout cell and embryo of mice. The interaction between the Cep164 and CP110 protein was confirmed by COIP. The results showed the Cep164 gene mutation increased CP110 gene expression. Cep164 regulated the expression of CP110 in cells indirectly. CP110 degradation failure could be the one of reason for the Cep164 gene mutation cause cilia dysfunction.

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Preliminary study on optimization of industrial layout in Gansu province based on water ecological constraints

LI Fei, ZHANG Xiao-ping

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 169-175. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.038

Abstract （374）

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In order to coordinate the relationship between water ecological environment and the development of regional industry, based on the two aspects of water resources and water environment, a zoning index system for the water ecological constraints in Gansu province was constructed, and a division and zoning method of the water ecological constraints was proposed. On the basis of water resource constraint zone and water environment constraint zone, through the two-dimensional quadrant analysis of water resources and water environment constraints in each region, the water ecological constraints of the whole province were divided into four types of water ecological constraint zones, including high water resources constraint and high water environmental constraint, high water resources constraint and low water environmental constraint, low water resources constraint and high water environmental constraint, low water resources constraint and low water environmental constraint, among them, the industrial layout of Jiayuguan city and Gannan prefecture has relatively good aquatic ecological adaptability. In this regard, the key transformation of industrial upgrading in Gansu province, the industrial environment access conditions, and the optimization of industrial layout are proposed. The research results can provide theoretical and methodological references for other areas to coordinate the relationship between industrial development and the relationship between water resources and water environment.

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Research on the evaluation of the protection of farmers’ rights by economic law on the PSR-AHP model

YANG Bei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 176-179. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.039

Abstract （447）

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The protection of farmers’ rights and interests is facing a major practical problem of the status of farmers in vulnerable groups. In order to prevent farmers from getting deeper and deeper in the vortex of vulnerable groups, it is necessary to specify the principles of economic law from the perspectives of balance and coordination, fairness and efficiency, extreme promotion and extreme restrictions, and to protect farmers' rights from this perspective. In order to evaluate the effect of economic law on the protection of farmers' rights and interests, the PSR-AHP model was introduced to construct an evaluation index system, and an empirical analysis was conducted using Shaanxi province as an example. The results show that economic law has a certain effect on the protection of farmers’ rights and interests, but there is a certain lag in the legislative and law enforcement levels. Future economic law reforms need to be improved from these two aspects in order to truly exert the effectiveness of protecting peasants' rights and interests.

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Analysis on the responses of college students’ innovation and entrepreneurship education to e-commerce poverty alleviation：Evidence from the Pearl River Delta and west Guangdong

WANG Ming-dui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 180-185. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.040

Abstract （494）

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In order to explore the internal mechanism of the response of college students’ innovation and entrepreneurship education in the Pearl River Delta and western Guangdong to e-commerce poverty alleviation, using the PSR model, and selecting 21 variables such as the rate of change in farmers’ income, with evaluation value conversion, comprehensive evaluation value measurement, coordination degree calculation and other methods, the pressure-state-response level of the study area was analyzed. The results show that the pressure of the study area is concentrating towards the Pearl River Delta, but Guangzhou, Foshan, and Shenzhen are still in a dominant position in the state and response. The negative correlation between the comprehensive evaluation score and the evaluation value of the degree of coordination in the study area shows that the crisis hidden in the region is widespread.

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A study on the supply of public services for farmers and herdsmen relocated from inhospitable areas——Based on the analysis of Wuqia county,Xinjiang Uygur Autonomous region

ZHOU Chong-chong, YANG Jun-xiao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 186-189. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.041

Abstract （671）

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Change of poverty alleviation relocation involved many aspects, such as public service, industry employment, Wuqia county in Xinjiang Uygur Autonomous region topaz port change of poverty alleviation and moving point of practical investigation and research, for example, summed up the public service supply results and analyzed its existing problems, and put forward to realize the diversification of the rural public service supply, improve the farmers demand expression ability, strengthen community cadre public service ability and so on suggestion.

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Research on the demand of knowledge payment products based on the group perspective of network interest

HE Li-shi, WANG Hong-peng, ZHU Hui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 190-193. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.042

Abstract （477）

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Based on net interest margin group perspective to study the knowledge characteristics of paid products and user demand, found that users use knowledge demand pay product was the use of fragmented time to accumulate interdisciplinary knowledge and skills, in order to solved the knowledge anxiety, screening high quality content and services, access to experted in the field of cognitive and insight, seek sentimental, relieve the huge pressure from society. By referring to the typical case of knowledge payment product demand to meet the design, put forward the knowledge payment product development Suggestions.

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Research on the path of farmers’ participation in rural tourism projects under the background of precision poverty alleviation：Based on a sustainable livelihoods framework

PAN Xin, ZHANG Jiao-jiao, LIU Yi-ran, LI Xue-ting

HUBEI AGRICULTURAL SCIENCES 2020, 59 (2): 194-200. DOI: 10.14088/j.cnki.issn0439-8114.2020.02.043

Abstract （593）

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Based on the sustainable livelihood framework, statistical analysis of the livelihood capital scores of 236 rural households in two state-level poverty-stricken counties, Zigui county, Hubei and Pingshan county, Hebei, a was taken, and the logistic model was used to explore the relationship between livelihood capital owned by farmers and rural tourism participation. The results show that social capital has the most significant impact on farmer participation in tourism projects, followed by financial capital, physical capital, and natural capital, and human capital is the least significant. The natural capital of Jiuwan Stream Scenic Area is significantly higher than that of Foguang Mountain Scenic Area, the difference between other capitals is not obvious, and it has similar effects on farmers' participation in rural tourism projects. Due to the lack of livelihood capital, the participation of rural poverty-stricken households is significantly lower than that of ordinary farmers. Efforts to increase the existing social and financial capital stocks of farmers are of great significance to motivate farmers, especially poor households, to participate in rural tourism projects, and at the same time help improve the livelihood results of poor households, thereby achieving precision poverty alleviation.

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Resarch progress of transcription mediator complex

WANG Wen, LIU Kun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 5-8. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.001

Abstract （589）

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The mediator complex, a highly conserved large complex with modular tissue, is widely distributed in eukaryotes and is involved in the transmission of information between transcription factors and RNA polymerase II. The main function of the mediator complex is to regulate the expression of downstream genes by interacting with transcription factors stimulated by different signaling pathways. In addition, the mediator complex interacts with various cofactors to participate in processes such as transcriptional elongation, mRNA export, and selective splicing, thereby controlling various physiological functions of the cell. The close relationship between mediator complexes and disease makes it possible to study the treatment of disease with mediator as a target. Based on the structure of the mediator complex, the molecular mechanism and function of the mediator complex in the regulation of gene expression were introduced, in order to provide reference for the following research.

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Advances in chemical compounds and pharmacological effects of Pilea cavaleriei Levl

CHEN Xiao-yi, PANG Yu-zhou, WANG Zhi-ping

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 13-16. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.002

Abstract （646）

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Pilea cavaleriei Levl. is the whole plant of urticaceae. It mainly contains sesquiterpenes and their glycosides, volatile oil and other components. It has the effects of clearing away heat and detoxifying, reducing swelling, diuresis, relieving phlegm and relieving cough. The chemical constituents and pharmacological effects of Pilea cavaleriei Levl. was reviewed in order to provide a basis for the further development and utilization of Pilea cavaleriei Levl..

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Research progress on Chinese yam micro tuber

XIANG Fa-yun, ZHANG Qing-hua, ZENG Xiang-guo, GUI Bao-chuan, GUO Cong, GUAN Wei, CHEN Feng-ying, CHEN Xi-yang, HAN Yong-chao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 17-20. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.003

Abstract （386）

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Chinese yam micro tuber is the main way to obtain the good Chinese yam virus-free varieties. The research progress of Chinese yam micro tuber at home and abroad was introduced, including the local varieties of Chinese yam, the significance of micro tuber research, the factors affecting the production efficiency of micro tuber, the precautions for micro tuber transplanting, the field growth of micro tuber, the problems existing in micro tuber production, and the prospect of application of micro tuber in production.

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Experiment report on maize-late rice cropping pattern in Jianghan plain

YANG Yan-bin, BA Si-he, XU Hua-yan, SUN Chen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 21-24. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.004

Abstract （818）

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Selecting early matured maize varieties such as Denghai 618, Weike 711, Zhengdan 958（CK）, silage maize variety of Yaqingzhu No.8, and fresh eating corn varieties of Yuetian 16, Jingkenuo 2000, Yuetian 28 and Yunuo No.7 in the spring sowing, followed by direct seeding a late japonica rice variety Tiejing 17 and an early autumn sown rice variety Liangyou 287 into corn stubble, farmers can work under a paddy-upland rotation system, and can get harvest of two crops twice a year. The maximum output of corn kernels of early matured maize sown in spring reached 8 820.0 kg/hm². The maximum output of direct seeding late japonica rice reached 7 722.0 kg/hm². The minimum output of kernels of two crops in two seasons reached 15 165.0 kg/hm². It is proposed that the growth period of early spring matured maize should be controlled within 120 days from March 20（sowing） to July 20 （ripening） with accumulated temperature of 2 600 ℃. The growth period of late japonica rice varieties should be controlled within 100 days with accumulated temperature of 2 360 ℃. The crops are direct seeding with stubble on July 20, full heading around September 17 and ripened before the end of October.

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Benefit analysis on new planting patterns of broad bean-organic sweetpotato with high efficiency in Xichong dryland

SHEN Xue-shan, QU Hui-juan, ZHOU Quan-lu, ZHU Ling, WANG Chang-tao, ZHENG Sheng-hua

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 25-28. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.005

Abstract （501）

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To research the yield and economic benefit of new planting patterns with sweetpotato in dryland, new double cropping planting pattern of broad bean-organic sweetpotato and traditional pattern of maize-wheat-sweetpotato（CK） were conducted in Xichong county, Nanchong city, Sichuan province. The results indicated that, compared with the local traditional patterns, the unprocessed food grain yield of new planting pattern of double cropping that broad bean-organic sweetpotato was decreased by 78.47%~84.08%, but the total output value of that were increased by 36.61%~49.46%, net output value of that were increased by 65.67%~87.38%, net benefits of that were increased by 3.64~4.64 times than control, agriculture labour net productivity of that were increased by 1.01~1.27 times. It can be seen that the new pattern of broad bean-organic sweetpotato has a good prospect in popularization and application in Xichong county and other similar regions.

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Effects of Cd²⁺ stress on photosynthetic physiology and antioxidant enzyme activities of Shangmai 1619 seedlings

WU Xiu-ning, CHE Li-ping, ZHANG Jun, ZHAO Yong-ping

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 29-32. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.006

Abstract （426）

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To explore the effects of Cd²⁺ stress on physiological characteristics of wheat（Triticum aestivum L.） variety Shangmai 1619 regional test control variety seedling, taking Shangmai 1619 and Xiaoyan 15 as tested materials, chlorophyll content, photosynthetic physiological parameters and antioxidant enzyme activities of wheat seeding under different Cd²⁺ stresses were determined under hydroponic conditions. The results showed that, after Cd²⁺ stress, chlorophyll content, net photosynthetic rate （P_n）, transpiration rate（T_r）, stomatal conductance（G_s）, intercellular CO₂ concentration （C_i）, superoxide dismutase （SOD） activity and peroxidase（POD） activity of two wheat cultivars all decreased. Catalase（CAT） activity and malondialdehyde（MDA） content in Shangmai 1619 increased, while that of Xiaoyan 15 decreased. Comparatively speaking, Shangmai 1619 showed weaker resistance than Xiaoyan 15 under Cd²⁺ stress.

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Study on evaluation of intensive use of agricultural land in Huanggang city

DAI Jie, GUO Lei, LIU Meng-ba

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 33-37. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.007

Abstract （421）

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Taking Huanggang city as a research area, using principal component analysis method and multi-objective decision-making method, the indicators of agricultural land input intensity, agricultural land use degree, agricultural land output effect and agricultural land sustainability were selected to construct agricultural land intensification in Huanggang city, and the intensive use of agricultural land was evaluation. On this basis, the intensive use of agricultural land in various counties （cities, districts） of Huanggang city in 2015 and the changes in the intensive use of agricultural land in Huanggang city from 2010 to 2015 was analyzed. The results showed that the comprehensive scores of intensive use of agricultural land in counties and districts of Huanggang city ranged from -0.78 to 1.22 in 2015, the overall intensive utilization level was in a medium-low state, and the intensive use of agricultural land in each county was different. Among them, the agricultural land in Huangzhou district and Luotian county was intensively utilized, the agricultural land in Yingshan county, Tuanfeng county and Huangmei county was generally intensively utilized, and the agricultural land in Wuxue city, Macheng city, Shachun county and Hong'an county was intensively utilized. The agricultural land in Qinshui county was extensively utilized. The comprehensive score of agricultural land intensive use in Huanggang city was between -0.40 and 1.15 from 2010 to 2015, and the intensive utilization degree showed an overall upward trend. The research results were in line with the actual use of agricultural land in the region, and corresponding suggestions based on the intensive use of cultivated land in Huanggang city were put forward, which had certain guiding significance for the efficient intensive and sustainable use of agricultural land in Huanggang city.

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Effect of potassium fertilizer application rate on yield and potassium use efficiency of rice-rape rotation system

SHAO Wen-sheng, ZHOU Xiong, HE De-zhi, TIAN Gui-sheng, LU Jian-wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 38-41. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.008

Abstract （455）

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Taking rice-rape rotation system as research object, the effects of potassium （K） application rates on rice-rape rotation system crop yield, K accumulation and use efficiency of rice and ripe were studied by fields experiments with K₂O rates of 0, 120 and 240 kg/hm². In which, the K₂O rate of 120 kg/hm² is recommended amount of potassium fertilizer for local soil test formulations. The results showed that crop yield of the rice-rape rotation system was increased at the first and then gradually decreased with the increment of the K application rates,and reached maxima under the K fertilizer application rate of K₂O 120 kg/hm². The K fertilizer utilization efficiency of rice and rape were decreased significantly with the increment of the K application rates. In conclusion, under this field experiment conditions, the optimal K fertilizer application amount is K₂O 120 kg/hm² in Wuxue city, Hubei province.

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Causes comparison analysis of environmental changes in Hunshandake in recent 15 years

GAO Jian, WEI Cheng-lin, NING Xiao-li

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 42-47. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.009

Abstract （531）

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Hunshandake sandland becoming one of the main sand and dust sources in Beijing and Tianjin region, and it threatened the social, economic sustainable development and ecological security around Beijing, Tianjin and north China. The natural environment, socio-economic characteristics and ecological environment of the Hunshandake sandland in 2001 and 2015 were compared, and the ecological status, climate change trends, and components of ecosystem components were analyzed to explore the causes of changes in the ecological environment in this region. The results showed that changes in temperature and precipitation in the Hunshandake sandland have less impact on the ecological environment of the area. Additionally, the number of livestock has shown a downward trend, the degree of desertification has been decreasing, and the ecological environment is improving gradually. Finally, the output of the primary industry was maintained at the same level for a long time. In recent years, although the ecological environment has improved, but still cannot be neglected the deteriorating areas. People must be increase the ecological awareness continuously, and continue to promote governance measures such as grazing prohibition and afforestation.

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Evaluation on land ecological security in mountains of northern Guangdong province based on principal component analysis

MA Jun-wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 48-52. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.010

Abstract （457）

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In order to explore the status of land ecological security in mountainous of northern Guangdong province, 14 indicators from the aspects of resource and environmental pressure, land use status and social economic development were selected by the principal component analysis method, then the evaluation index system with county administrative division as evaluation unit was built to comprehensively evaluate the land ecological security in the mountainous of northern Guangdong province. The results showed that the comprehensive score of land ecological security in the mountainous of northern Guangdong province was 0.477 9, which was in the level Ⅲ, critical safety. The spatial ecological security level of this area showed the regional difference that west was high and east was low in spatial distribution. Population density and economic development level were the main factors affecting the ecological security of mountainous in northern Guangdong province, and have a negative correlation with the level of land ecological security.

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GIS-based zoning of geological hazard’s susceptibility in Ankang city of Shaanxi province

ZHEGN Zhi-guo, GUO Jun-li, LI Ping-lu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 53-57. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.011

Abstract （458）

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Based on the survey of geography disasters in Ankang city of Shaanxi province, an evaluation index system by choosing annual precipitation, slope, elevation, point density of disasters, soil type and human engineering activities was established. Adopting information analyzing model of GIS, the susceptibility zones of geography disasters in Ankang ctiy was made. The results show that Ankang ctiy could be divide into four geological hazard zones, including high-incidence zone, middle-incidence zone, low-incidence zone and nonoccurrence zone. Among the zones, the area of high-incidence zone is 6 135 km², covering 26.52% of the total area; the area of middle-incidence zone is 8 096 km², covering 35.01% of the total area; the area of low-incidence zone is 6 488 km², covering 28.05% of the total area; the area of nonoccurrence zone is 2 411 km², covering 10.42% of the total area.

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Comparative analysis of two strong snowstorms in the northeast of Hunan province in 2018

YIN Yi-wen, JIANG Shuai, XU Li-li, CHANG Li-wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 58-66. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.012

Abstract （361）

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Based on the NCEP/NCAR reanalyzed data and the conventional observational meteorological observing station data, two strong snowstorms in Hunan province in 2018 were comparatively analyzed. The results showed that both snowstorms were caused in "Ω" circulation situation at 500 hPa, but there was a significant difference in the middle-low atmosphere. The first process （January 24 to 31, 2018） had a shear line and a good vapor condition, and the warm and wet air were forced to climb, because the cold air wedged in from the near ground. In the second process （December 28, 2018 to January 3, 2019）, a strong and stable cold pad had been formed before the snow, and the warm and wet air climbed along the pad at 850 hPa. The snowstorm areas were located in the left side of the large water vapor flux area and near the vapor flux divergence center. The main reason for the difference of water vapor is the different sources of water vapor by the Lagrange air parcel tracking method. The water vapor in the first process mainly came from humidified northerly backflow which through the Yellow Sea and the East China Sea from the bottom of the high pressure and the Arabian Sea, the second process was from Indo-China peninsula and India peninsula. The two processes both had pseudo-equivalent potential temperature intensive zone and the surface front zone near the 22° N. The slope of the second process was higher than that of the first process and the wet air climb obviously, which caused the low-level cyclonic vorticity increases, and the relative humidity, the relative vorticity and vertical movement extend upward significantly at 12 hours before the start of heavy snowstorm, which has precursory significance for the prediction.

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Comparative study on acute oral toxicity of water-soluble fertilizers of different types in mice

YANG Tong-jin, TENG Jing-jing, ZHENG Si-yu, FENG Xue-qing, BAO Jun-hui, WANG Yan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 67-70. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.013

Abstract （413）

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Using the commissioned samples of water-soluble fertilizers received by Anhui Provincial Center for Disease Control and Prevention, the acute oral toxicity tests were carried out according to Fertilizer Registration-Determination and Evaluation of Acute Oral Toxicity（NY 1980—2010）, and then the acute oral toxicity of five different types water-soluble fertilizers in mice were compared, so as to provide the basis for fertilizer selection and treatment of fertilizer poisoning personnel. The results showed that the major element water-soluble fertilizers, amino acid water-soluble fertilizers and humic acid water-soluble fertilizers were actually non-toxic, the medium element water-soluble fertilizers were actually non-toxic or low toxicity, and the trace element water-soluble fertilizers belonged to low toxicity.

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Application of three cluster methods on regionalization of temperature and precipitation in Hunan province

LI Yi-zhi, DAI Ze-jun, ZHU Yu-xiang, LUO Bo-liang, DENG Jian-bo

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 71-78. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.014

Abstract （421）

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Based on the monthly temperature and precipitation data from 90 stations in Hunan province from 1961 to 2015, the eigenvectors of annual mean temperature and annual total precipitation were extracted by REOF method. Based on this, K-means clustering method, sample system clustering method and fuzzy C-means clustering method were used to partition, and the most reasonable partitioning results were selected. The results show that the annual average temperature in Hunan province is divided into four zones： northeastern Hunan, northwestern Hunan and western Hunan, central Hunan and southern Hunan, and southeastern Hunan; The precipitation in Hunan is divided into six zones： northwestern Hunan, northeastern Hunan, western Hunan, southwestern Hunan, central Hunan, and southern Hunan.

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Preparation of antimicrobial plant medium and research of its antibacterial properties

ZHANG Xin, CHEN Jing-wen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 79-81. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.015

Abstract （425）

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Nanosilver particles were obtained by liquid phase reduction of AgNO₃, and nanosilver composite plant medium with good antimicrobial capability was successfully prepared to realize open culture of plant tissues. The experiment mainly included the preparation of nanosilver particles and the preparation of composite plant medium. First, nanosilver particles were prepared in AgNO₃ solution using fructose and D-glucose as reduction reagents. The effect of AgNO₃ concentration on the nanoparticle size was investigated. Second, the solvent containing the nanosilver particles was added in B5 medium to form a composite plant medium. It was found that with the increase of AgNO₃ solution concentration, the average size of nanosilver particles increased, and the size distribution range became wider. Moreover, the composite B5 medium containing the nanosilver particles exhibited significant antimicrobial capability.

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The field efficacy test of different insecticides with one-time application to sugarcane borers

LUO Ya-wei, QIN Zhen-qiang, LI De-wei, LIANG Tian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 82-86. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.016

Abstract （502）

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In order to reduce the usage amount and frequency of insecticide on sugarcane borers, the field efficacy of six different insecticides with one-time application on borers and its effect on sugarcane yield and brix were studied. The results showed that there had better control efficacy on the borers in new-plant seedling when spray the 10% clothianidin·monosultap granules of 75 kg/hm², 30% chlorantraniliprole·thiamethoxam suspending agent of 600 mL/hm², 2% imidacloprid granules of 75 kg/hm², 3.6% insecticidal double particle and 60% dinotefuran dispersible powder of 150 g/hm² on sugarcane with one-time application. The control efficacy of 10% clothianidin·monosultap granules of 75 kg/hm² and 30% chlorantraniliprole·thiamethoxam suspending agent of 600 mL/hm² were more than 70% after applied for 45 days and 60 days. In ratoon sugcarcane, the control efficacy of 10% clothianidin·monosultap granules of 75 kg/hm² and 30% chlorantraniliprole·thiamethoxam suspending agent of 600 mL/hm² on borers also were higher than the other insecticides tested. There were no significances on brixs, effective stems and yields of sugarcane in new-plant among the six insecticides compared the control without use insecticide. On the other hand, there were no significance on brixs and effective stems of sugarcane in ratoon among the six insecticides compared the control without use insecticide. While the yield of sugarcane with spraying 10% clothianidin·monosultap granules of 75 kg/hm² was the highest compared the other treatments tested. There have a significant increase of 17.5% in yield when apply 10% clothianidin·monosultap granules of 75 kg/hm² compared the control without use insecticide. The results from this study indicated that there have better control efficacy of 10% clothianidin·monosultap granules of 75 kg/hm² on sugarcane borers, and it can increase the sugarcane yield and production efficiency.

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The application research on killing Lasioderma serricorne by CO₂

SUN Jian-feng, YANG Fen-yu, LI Zhen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 87-90. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.017

Abstract （514）

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In order to discuss the new non-toxic approaches of preventing tobacco pests, it is suggested to use bottled CO₂ as a medium to fill the CO₂ gas in the sealed disk, set different parameter gradients, and use live insects to test the effectiveness. The amount of leaf water, appearances and chemical composition were analyzed to evaluate the feasibility of using CO₂ to kill Lasioderma serricorne, to ensure the effects and the quality of tobacco storage. The results show that when the temperature conditions meet the requirements of insect control, and the oxygen concentration in the sealed stack is less than 2% and maintained for more than 35 d during CO₂ treatment, it can effectively kill Lasioderma serricorne.

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Screening and supporting cultivation techniques of high-yield varieties of vegetable soybean in Chongqing city

ZENG Xin-yu, ZONG Hong-xia, ZHANG Li, SUN Xue-ying, ZHU Ti-chao, Huang Cheng-wen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 91-94. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.018

Abstract （506）

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Eight vegetable soybean varieties were introduced from China, and their economic traits in Chongqing were studied from 2016 to 2017, and high-yield cultivation techniques were studied in 2018. The results showed that Zhejiang Fresh Bean No. 9 and Jiaoda 11 performed well and were suitable for planting in Chongqing. The amount of fertilization, field density, and sowing date of Zhejiang Fresh Bean No. 9 had significant effects on yield, and late sowing had a big impact on yield. Combining with the actual production, the high-yield production technology for vegetable soybeans in Chongqing are proposed from aspects of variety selection, site selection, site preparation, planting date determination, density control, water and fertilizer, weed management, harvest period determination, and pest control.

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Effects of different supplementary pollination methods on seed yield quality of tobacco

ZHANG Jun-jie, LI Zong-ping, XU Shi-ping, PENG Hao, CAO Jing-lin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 95-96. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.019

Abstract （407）

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The flue-cured tobacco variety Yunyan 87 was used as the test material. The effects of artificial rocking, soluble starch medium pollen-assisted pollination, and sucrose liquid-assisted pollination on the yield and quality of tobacco seeds were compared. The results showed that compared with natural pollination, the yield of tobacco seeds and the number of single fruit grains were significantly increased. Among them, soluble starch media pollen-assisted pollination and artificial rocking are particularly effective in increasing tobacco seed yield and single fruit number, which are 39.7%, 87.4%, 28.4%, and 82.5% higher than the control, respectively, which are significantly different from the control. Different auxiliary pollination methods had no significant effect on tobacco seed quality. Artificial shaking is a cost-effective auxiliary pollination method.

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Effects of aluminum and temperature on quality of Liubao tea

YE Jin-pei, HUANG Ai-ping, TANG Shi-bin, LI Zhi-qiang, LI Xiao-li, NIE Hai-quan, CHEN Shi-xiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 97-101. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.020

Abstract （424）

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Using Liubao tea group seedlings as materials, under different hydroponic conditions, different aluminum concentrations （0, 50, 100, 200, 400 mg/L） and temperature （5, 15, 25 ℃） was set to treat Liubao tea seedlings. The effects of aluminum and low temperature on the contents of tea dry matter, free amino acids, tea polyphenols, tea polysaccharides and water extracts were studied. The results showed that except for dry matter, the content of other substances increased first and then decreased with the increase of aluminum concentration. The effect of aluminum concentration on dry matter, free amino acids, and water leaching content was small, and there was no significant difference between treatments. Appropriate amount of aluminum can promote the quality of tea to a certain extent; the effect of 100 mg/L treatment is obvious, but excessive aluminum will affect the quality of tea. Except for tea polyphenols and water extracts, temperature had little effect on the contents of dry matter, free amino acids, and tea polysaccharides, and there was no significant difference between treatments. The dry matter and tea polyphenols treated at 5 ℃ are slightly lower than those treated at 25 ℃. The free amino acids, tea polysaccharides and water extracts in tea treated at 5 ℃ and 15 ℃ are higher than those treated at 25 ℃, especially the tea polysaccharides and water extract at 5 ℃. Temperature has a certain effect on the quality of Liubao tea. Treatment of Liubao tea with different concentrations of aluminum or temperature will change the tea quality to some extent.

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Studies on adaptability of seven species of Gesneriaceae plants to light intensity

XU Hui, TU Ji-hong, LIANG Yu-ting, ZHANG Xiao-qin, LI Na, LI Peng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 102-107. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.021

Abstract （471）

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The adaptability of the light intensity of the Gesneriaceae plants was studied to improve the quality of potted Gesneriaceae plants and promote its reasonable application in gardens. The physiological indicators such as malondialdehyde, soluble protein and chlorophyll content under different shading conditions of seven Gesneriaceae plants, including Primulina fimbrisepala, P. eburnean, Primulina ‘Silver Antler’, Hemiboea gracilis, Briggsia mihieri, Lysionotus pauciflorus and Pseudochirita guangxiensis were measured, and the growth was observed. Comprehensive analysis and comparison show that all 7 species of Gesneriaceae family have strong negative tolerance and can grow normally under the conditions of light intensity of 2 000~8 000 lx. From light to strong, the adaptability to the light is Lysionotus pauciflorus, Pseudochirita guangxiensis, Primulina 'Silver Antler', Briggsia mihieri, Primulina fimbrisepala, Hemiboea gracilis, Primulina eburnean.

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Effects of planting years on leaf and root traits of Codonopsis pilosula in Luolong

LI De-hui, LI Xian-bi, ZHANG Bao-cheng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 108-110. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.022

Abstract （370）

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Some traits of leaves, roots and roots of Codonopsis pilosulae grown in Luolong township, Daozhen county, Guizhou province were studied, the results showed that with the increase of planting years, the leaf chlorophyll a showed a significant decrease trend. There were no significant differences in soluble polysaccharides and soluble proteins in the roots of C. pilosula grown for 2, 3, and 4 years. With the increase of planting years, the quality of single plant increased, and the proportion of xylem increased. Lignification will affect the taste of C. pilosulae. Care should be taken to promote the cultivation of C. pilosula as a medicinal and food plant at an altitude of 1 500 m.

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Effect of feeding Gynostemma Pentaphyllum on the thymus aging of Guangxi Sanhuang chickens

ZHANG Run-xiang, WU Ya-hui, HUANG Shi-qi, WANG Yong-jie, BAI Xi, TANG Xiao-chuan, WANG Xiao-li

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 111-114. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.023

Abstract （391）

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From the histological point of view, the effect of adding Gynostemma pentaphyllum on the thymus aging of Guangxi Sanhuang chickens was studied. 0.5% G. pentaphyllum powder was added to the feed, and the thymus weight, thymus index, cortex-medium area ratio, number and area of thymus bodies were examined at 1, 14, 28, 56, 90, 120, and 150 d. The results showed that with increasing age, the thymus weight increased, and the thymus index rose first and then decreased. The addition of G. pentaphyllum increased the thymus weight and thymus index to a certain extent. With the increase of the age, the thymic cortex-medullar ratio decreased, and at 120 d, the cortex-medullar ratio of the fed group was significantly greater than that of the non-added group（P<0.05）. The number of thymus bodies in the non-feeding group and the feeding group increased before 56 and 90 d, respectively, and then decreased in a wave pattern. At 28, 90, and 120 d, the number of thymus bodies in the fed group was significantly more than that in the non-fed group（P<0.05）. The thymus body area of the non-feeding group and the feeding group increased before 120 d, and then decreased. The day-to-day differences of the non-feeding group and the feeding group were not significant. Adding G. pentaphyllum powder in the feed can prolong the thymus maturation time, delay the thymus degradation, and improve the thymus function.

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Effect of Coptis chinensis extract on TNF-α and IL-6 contents in serum of mice infected with five pathogens

ZHANG Ru, YANG Zheng-teng, MA Jia-bao, CUI Jian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 115-117. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.024

Abstract （392）

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The effects of oral administration of Coptis chinensis extract on the contents of serum TNF-α and IL-6 in mice with acute abdominal infection induced by 5 common bacteria, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, and Klebsiella pneumonia, were investigated. The results show that the high, medium and low dose groups of Coptis chinensis extract have protective effects on mice with abdominal infection caused by S. aureus, E. coli, P. aeruginosa, P. vulgaris, and K. pneumoniae, can significantly reduce the content of TNF-α and IL-6 in mouse serum. Coptis chinensis extract has a certain antibacterial effect, and its mechanism is related to TNF-α and IL-6.

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Study on extracting and antioxidant activity of total flavonoids from Evodia rutaecarpa

ZHAO Nan, JING Yun-rong, HE Ting-ting, CHAI Jun-hong, WAN Chun-lei, ZHANG Lei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 118-121. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.025

Abstract （382）

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Taking Evodia rutaecarpa as raw materials, and total flavonoids content as the index, on the basis of single factor experiment, the optimization of extraction of flavonoids from Evodia rutaecarpa was investigated by orthogonal experimental design. The optimum conditions were ethanol concentration at 70%, solid-liquid ration at 1∶50, extraction time at 40min and extraction temperature at 65℃. The results showed that the extracting rate of total flavonoids reached 8.82%. The antioxidant activity of flavonoids from Evodia rutaecarpa had good ability to scavenge DPPH· free radical.

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Experiment on fermentation of biogas by two kinds of cassava alcohol wastes PHIMPHANH

Vilaiya, WANG Chen, MA Wen-qin, ZHENG Xin, JIA Zheng-yan, XIA Ying-chun, JI Jun-lin, YIN Fang, ZHANG Wu-di

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 122-125. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.026

Abstract （426）

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It uses the dried distiller's grains remaining after the fermentation of Chinese cassava and Lao cassava to produce alcohol. The batch biogas fermentation experiment is carried out under the condition of（35±1） ℃. The concentration is 4%. The experimental results show that the average daily gas production of Chinese cassava distiller's grains and Lao cassava distiller’s grains is 158.2,149.3 mL/d, respectively. The TS gas production rate and VS gas production rate of Chinese cassava distillers’ grains were 659,867 mL/g, respectively. The TS gas production rate and VS gas production rate of Laos cassava distiller’s grains are 799,1 080 mL/g, respectively. The average methane content in biogas was 50% and 51%, respectively.

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Determination of 3 quinoxaline drugs in hotpot seasoning by UPLC-MS/MS

MA Xiao-nian, DUAN Hai-bo, ZOU Yan-li, ZHANG Xiu-qing, LIANG Zhi-jian, CHEN Jun-xiu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 126-129. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.027

Abstract （395）

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UPLC-MS/MS was used to simultaneously detect three quinoxaline drugs in the hotpot seasoning. After the sample was extracted by acetonitrile ethyl acetate, acetonitrile-0.2% formic acid water was used as the mobile phase, and the multi reaction detection positive ion mode was used for qualitative and quantitative analysis. The results show that the linear relationship of the three quinoxaline drugs is good, the correlation coefficient is above 0.999, the average recovery of different concentration is 66.5%~84.4%, and the average relative standard deviation is 6.8%~9.2%. This method is suitable for the detection of residual pollution in hotpot seasoning.

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Study on quality standard of Chinese medicine compound shufu cream

ZHAO Yu, SHI Lei, ZHANG Qian-yu, YANG Jia, YANG Hong-bing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 130-133. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.028

Abstract （529）

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TLC was used to qualitatively identify Nepeta cataria L, Saposhnikovia divaricata （Trucz.） Schischk, Campsis grandiflora and Euphorbia fischeriana Steud. The content of the active constituents of fraxinellone and obacunone from cortex dictamni was determined by HPLC. The results of TLC were ideal, and the negative samples had no interference. The linear relationship between the anthrone and the peak area was good （r=0.999 1） and the average recovery was 99.76% （RSD=1.34%） in the concentration range of 5.92~47.36 μg/mL. In the concentration range of 10.2~81.6 μg/mL, the linear relationship between the ketone and the peak area was good （r=0.999 0）, and the average recovery was 99.89% （RSD=1.63%）. This method is easy to operate, has good specificity, accurate and reliable, and can be used for quality control of traditional Chinese medicine compound skin cream.

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Analysis of volatile constituents in Ajuga ciliata Bunge. by SD-HS-SPME

MA Yin-yu, LU Jin-qing, HAN Man, DU Cheng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 134-136. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.029

Abstract （409）

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Using HS-SPME and SD to extract volatile components from bone grass and using GC-MS to analysis them. The results showed that 14 peaks were separated by HS-SPME-GC-MS, and 12 components were identified. A total of 20 peaks were analyzed by SD-GC-MS, and 16 components were identified. HS-SPME and SD combined with GC-MS technology is applicable to the analysis of various types of volatile components, and can be used to analyze more comprehensive information on volatile ingredients.

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Analysis of volatile components in Nelumbinis stamen and Nelumbinis plumula by HS-SPME-GC-MS

WANG Wen-tong, JIANG Han-mei, XUAN Fei-yang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 137-140. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.030

Abstract （408）

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HS-SPME-GC-MS was used to analyze the volatile constituents of Nelumbinis stamen and Nelumbinis plumula in different medicinal parts of lotus, so as to make rational and effective use of traditional Chinese medicine. The results showed that 24 kinds of volatile components were detected in lotus seed, the highest content was D-Limonene（14.12%）; 26 kinds of components were detected in lotus seed, the highest content was D-Limonene （52.63%）; 9 kinds of common volatile components were hexanal, nonanal, D-Limonene, alcanfor, decanal, 1-caryophyllene, pentadecane, g-terpinene and phenylethyl alcohol in lotus seed.

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Study on innovative extraction condition and analysis method to contents of ammonium in soil

LYU Bao-yu, BAI Hai-qiang, PAN Yan, LAN Yue-cun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 141-143. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.031

Abstract （538）

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A innovative method for the determination of contents of the ammonium in the soil by gas phase molecular absorption spectrometry （GPMAS） was proposed. The orthogonal experiment was used to optimize the extraction conditions of ammonia nitrogen in soils such as extraction temperature, extractant concentration and extraction time. The effects of extraction conditions on the amount of ammonia nitrogen extracted from soil were studied. The results showed that the optimum conditions for ammonia nitrogen extraction in soil were as follows： KCl extract concentration was 2 mol/L, extraction temperature was 25℃, and extraction time was 30 min. The standard sample was recovered by gas phase molecular absorption spectrometry. The recoveries of the actual samples were 88.0%~105.5%, and the relative standard deviations were 3.8%~4.3%. Compared with the current industry standard methods, there was no significant difference in the results of the test by t test. The determination method is simple and easy, and can meet the requirements of large-scale determination contents of soil ammonia nitrogen, and has practical promotion value.

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Study on RNA difference of Xinjiang red meat apple extracted by different methods

TANG Lei, TONG Pan-pan, ZHANG Ya-ruo, WANG Fang, TANG Zhang-hu, LI Wen-hui, YUAN Zu-sheng, WANG Jiang-bo

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 144-146. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.032

Abstract （429）

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The total RNA extraction and purification kit， Trnzol method and improved CTAB method were used to extract the total RNA， from the pulp， pericarp， petals and leaves of red meat apple Kizi Alma， and the results of yield， quality and integrity of the three methods were compared. The final results showed that the purity of RNA extracted by Trnzol method was low， the degradation phenomenon， and the pollution of DNA， protein， the yield of pulp and pericarp was not ideal， but the RNA extracted by improved CTAB method and kit method was rarely degraded. The 28 s rRNA and 18 s rRNA bands were clear， the OD260/280 of petals and leaves was between 1.8 and 2.5， the yield of RNA extracted by raw reagent kit method was high， the ratio of OD260/280 and OD260/230 was between 2.0 and 2.5， and the integrity of the bands was good. Compared with the three methods， the clarity， yield and quality of RNA bands extracted by modified CTAB method and TrNzol method were lower than those by raw reagent kit method.

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Cloning and bioinformatics analysis on nifH gene of endophytic nitrogen-fixing bacteria Klebsiella variicola GN02 isolated from Pennisetum giganteum

LIN Biao-sheng, ZHANG Huan-xing, HE Yu-qin, LUO Mao-chun, LIN Zhan-xi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 147-153. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.033

Abstract （447）

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To research the bioinformatics on the nifH gene of endophytic nitrogen-fixing bacteria Klebsiella variicola GN02 isolated from Pennisetum giganteum, the nifH gene of GN02 strain was cloned and its integrity was verified, and bioinformatics prediction and analysis of nifH gene were carried out by a variety of online biological software. The results showed that the nifH gene of GN02 strain had a complete ORF, length of 882 bp and encoded 293 amino acid, and the gene accession numbers registered in NCBI GenBank was MK131264. Bioinformatics analysis showed that the nifH gene of GN02 strain was highly conserved and had more than 95% homology with the nucleotide sequence and amino acid sequence of nifH gene from other species. The total relative molecular weight of nifH protein was 31 970.73 and the theoretical isoelectric point pI was 4.72. The transmembrane model inclined to the inward to outward transmembrane of N-end, and the whole polypeptide chain was hydrophilic. nifH protein had two glycosylation sites but no signal peptide secretion, which may had a helical crimp structure. The prediction of secondary structure showed that α-helix and irregular curl were the main structural elements in the polypeptide chain of nifH protein, and the extended chain was scattered in the whole protein. Three-dimensional homologous modeling of nifH protein can be carried out in the database.

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Evaluation of ancient architecture value based on contingent valuation method

LI Min

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 154-157. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.034

Abstract （539）

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Ancient buildings not only have economic value, but also have historical, cultural, aesthetic and other values. A comprehensive assessment of their true value is conducive to the protection of ancient buildings. Taking an ancient building in Longshan county as an example, the public's willingness to pay for the protection and repair of the ancient building was investigated, and the value of the ancient building was measured using conditional value method. The results show that 68.45% of the respondents are willing to pay to protect and repair ancient buildings; respondents' average willingness to pay for the protection and repair of ancient buildings was 13.812 yuan; the value of the ancient building calculated based on the conditional value method is 14.78% higher than that measured by the cost method. Therefore, the conditional value method is a reasonable and feasible method for evaluating the value of ancient buildings.

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Health evaluation of urban ecological economic system in Mianyang city based on emergy analysis

DENG Jin-shang, XIANG Xiao-yu, DING Peng-kai, SHAO Huai-yong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 158-164. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.035

Abstract （392）

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Based on emergy analysis theory, a health evaluation index for urban eco-economic systems is constructed. The emergy analysis method is used to uniformly measure the relationship between energy flow, material flow, currency flow, and population flow and perform quantitative analysis. From the perspective of time and space, the health status and changes of the urban eco-economic system in Mianyang city were evaluated, and then suggestions and countermeasures suitable for the sustainable development of the eco-economic system in Mianyang city were put forward. The results show that from 2009 to 2016, Mianyang city's economy developed rapidly and its comprehensive strength increased significantly. Both the emergy investment rate and the power emergy ratio have been increasing year by year, while the net emergy output rate has continued to decrease, with a year-on-year decrease of 40% in 2016. The environmental pressure in the system is constantly increasing, the environmental load rate has increased from 1.52 to 2.39, the EISD has decreased from 6.23 to 4.68, and the EUEHI has a trend of rising first, then decreasing and then rising. The sustainable development capacity of the system is gradually weakening, and the health capacity of Mianyang city shows an unstable state. In order to maintain the sustainable development ability and health index of Mianyang's eco-economic system, it is necessary to further optimize the industrial structure in the future, improve the utilization of energy value of the resources; vigorously develop high-tech industries, reduce environmental load; change the model of eco-economic development, and realize the harmonious development of urban health and ecological environment.

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Demographic characteristics,policy support environment and entrepreneurial willingness of suburban farmers

XIAO Jing-yi, YAO Lei, XUE Yong-ji

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 165-169. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.036

Abstract （369）

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Compared with farmers in remote areas, suburban farmers have more convenient conditions for entrepreneurial under the radiation and promotion of big cities, and their entrepreneurship has positive practical significance for narrowing the gap between urban and rural areas and promoting urban-rural integration. From the perspective of demographic characteristics and policy support environment, an empirical study of influential factors of rural farmers' entrepreneurial intention is conducted. The results show that age has a negative correlation with suburban farmers' entrepreneurial intention, the number of households and annual income have a positive impact on suburban farmers' entrepreneurial intention. Suburban farmers with local household registration have a stronger willingness to start a businesses. The policy support environment, technical work experience and training experience are all positively related to entrepreneurial willingness.

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Research on industrial structure optimization and economic growth of manufacturing industry in resource-based regions

SHI Xue-qiao, GUO Xin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 170-174. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.037

Abstract （447）

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At present, the optimization of the industrial structure of the manufacturing industry is the focus of economic development in resource-based regions. Based on the panel data of manufacturing industries in six provinces in resource-based regions from 2005 to 2016, the nonlinear relationship between the optimization of manufacturing industry structure and regional economic growth was analyzed from the panel data model and panel threshold regression model. The results show that both the rationalization and the heightening of the manufacturing industry structure have significant threshold effects on economic growth. The higher the level of heightening, the greater the elasticity coefficient of economic growth. When the level of rationalization is low and high, the effect on economic growth is not obvious. Only at a moderate level it will play a positive role in economic growth. At present, the realization of the heightened industrial structure is still the focus of the optimization of the industrial structure of the manufacturing industry in resource-based regions. At the same time, rationalization needs to be placed on a more important position.

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A study on the influencing factors of farmers and herdsmen's demands for production and life difficulties：An empirical analysis based on the binary logistic regression model

QIN Shan-qi, ZHANG Ye, LIU Jun-yan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 175-179. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.038

Abstract （464）

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Based on the questionnaire survey data of 280 farmers and herdsmen in 62 villages in Yutian county, Hetian district, Xinjiang, a binary logistic model was constructed to analyze the influencing factors of farmers' and herdsmen's demands in production and life and explore the countermeasures and suggestions to further solve the production and living difficulties of farmers and herdsmen. The results show that the variables such as marital status, adequacy of feed, number of training sessions, and housing safety have a significant positive impact on whether farmers and herdsmen have difficulties in production and life. The number of households, whether the annual income is stable, the main source of income, the role of livestock and poultry, whether the sales of agricultural products are smooth, and whether there are agricultural and pastoral machinery have a significant negative impact on whether farmers and herdsmen have difficulties in production and life. Therefore, countermeasures and suggestions are proposed to liberate the labor force, strengthen the technical training of farmers and herdsmen, expand the sales channels of agricultural products, improve the infrastructure construction of agricultural and pastoral areas, and improve the basic medical and health service system.

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Research on the influencing factors of consumption willingness of green agricultural products based on S-O-R Theory

XU Han-min, HE Yu-cheng, YANG Yu-rong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 180-184. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.039

Abstract （494）

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Based on the S-O-R theory and perceived value theory, a model of the consumer's willingness to consume green agricultural products is constructed, and the influence of internal factors （health awareness, face concept） and external factors （subjective norms） on consumers' willingness to consume green agricultural products is discussed. The results show that consumers' health awareness, face view, and subjective norms have a significant positive impact on their willingness to consume green agricultural products.

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Investigation and analysis of peasant households' willingness to participate in land circulation cooperatives in Kuisu town of Balikun county

AN Rui, AYITUERXUN·SHAMUXI, PARUKE·WUSIMANJIANG

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 185-188. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.040

Abstract （388）

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Taking Kuisu town in Balikun county as the research area, the operation of the land transfer cooperatives and the willingness of farmers to participate were analyzed through field surveys. The results show that the degree of participation of farmers who transfer land to professional cooperatives is not high, but their willingness to participate is relatively high. Compared with pure farmers, farming and business farmers have a greater possibility to participate in farmer professional cooperatives. Most farmers still depend on land for farming; and farmers have a strong sense of protection of their own interests when transferring land. The main reasons for farmers not participating in cooperatives include poor understanding of the joining procedures and the lack of cooperatives nearby. After participating in the cooperative, the family's annual income increased significantly.

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Economic benefit analysis of integrated rice,shrimp and finless eel breeding

LIANG Jie, ZHENG Guo-hong, LYU Ya-bing, WANG Xiao-ning, ZHANG Qi, CHEN Jie-yu, GOU Jing-wei, HE Xu-gang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 189-192. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.041

Abstract （426）

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The growth status and economic benefits of finless eel （Monopterus albus） and shrimp（Procambarus clarkii） in rice-shrimp-finless eel co-cropped rice fields with three stocking densities of finless eel （3 000, 6 000, 9 000 tails/hm², each with 3 parallels） and rice-shrimp co-cropped rice fields （control group） were compared. After 109 days of breeding, the weight of P. clarkii increased from an average of 5.13 g/tail to 30.4 g/tail. M. albus gains from an average of 42.6 g/tail to 132.5 g tail; and the recovery rate reached 79.1%. The net profit of the three test groups and the control group was 79 120, 72 882, 63 213, and 50 820 yuan/hm². The rice yield of the rice-shrimp-finless eel group was significantly higher than that of the rice-shrimp group, and the yield of P. clarkii was slightly lower than that of the rice shrimp group, but there was no significant difference between the two groups. The terminal body weight and weight gain rate of P. clarkii were significantly lower than those of the control group, indicating that proper stocking of M. albus in shrimp paddy fields would affect the specifications and yield of P. clarkii to some extent. However, it has brought about an increase in rice production and additional income from M. albus, which can significantly increase the comprehensive economic benefits of shrimp and paddy fields.

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Thoughts on the building of advantage areas of characteristic agricultural productsand high-quality development of the animal husbandry in the Mufu mountain area, Hubei province

SUN Zhi-guo, XIONG Wan-zhen, DING Guang-ping, LIU Zhi-yang, ZHONG Ru-gang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 193-196. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.042

Abstract （470）

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The general situation of national characteristic agricultural products superior area of animal husbandry in China was introduced, the advantages of animal husbandry products and their biological genetic resources were analyzed in the Mufu mountain area of Hubei province,the tactics were discussed to build advantage areas of characteristic agricultural products, and promote high-quality development of the animal husbandry in the poor area under the rural revitalization strategy and the targeted poverty alleviation strategy.Some suggestions on establishing 6 provincial-level and 4 national-level preponderant areas of characteristic agricultural products were put forward.

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Analysis on the coupling and coordination of industrial development and targeted poverty alleviation in deep poverty areas——Taking Qinba Mountain Area in south Shaanxi as an example

GUO Meng, WANG Yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (3): 197-200. DOI: 10.14088/j.cnki.issn0439-8114.2020.03.043

Abstract （393）

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Deep poverty-stricken areas are the backbone of poverty alleviation. Taking Qinba Mountains in southern Shaanxi which is a concentrated area of extreme poverty as an example, the development of agriculture, tourism and precise poverty alleviation in Qinba Mountains in southern Shaanxi were evaluated by building a coupling model and using the method of entropy to determine the weights based on the data of industry and poverty alleviation in the three cities of southern Shaanxi from 2009 to 2018. The results showed that the coupling of agriculture, tourism development and targeted poverty alleviation in Qinba mountain area was stable, but the coupling degree was not high, and there was a large space for improvement, which needs further in-depth integration and interactive and coordinated development.Based on this,the policy suggestions were puts forward from three aspects, grasping policy opportunities, strict quality standards and increasing investment in science and technology, promoting industrial transformation and upgrading, promoting regional economic development, and achieving poverty alleviation and rural revitalization.

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Structure and signal regulation mechanism of phytochrome

ZHANG Yuan-yuan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 5-9. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.001

Abstract （1807）

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Light signals participate in and regulate multiple reactions in plant life cycles, and plants can obtain diverse optical information through their own photoreceptors. Phytochrome(phy) is one of the phytochrome proteins that sense red/far-red light, which plays an important role in developmental transitions such as germination and flowering, and adaptive responses such as phototropism and shade avoidance. Phytochromes exist in two interchangeable forms, the Pr form absorbs R light, whereas the Pfr form absorbs FR light, and this difference is evident in the structure of them. The structural differences and signal regulation mechanisms of the two forms of photopigmentation are reviewed. Moreover, we take the regulation of phytochrome on plant photosynthesis as an example to illustrate the significance of phytochrome for plant growth and development. Finally, the directions for future studies are discussed.

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Research progress of quorum sensing in Staphylococcus aureus

HONG Zheng-shan, HUANG Yu-mei, YANG Ke, ZENG Chun-hui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 10-12. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.002

Abstract （489）

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The known group effect systems in Staphylococcus aureus are the Agr system and LuxS/AI-2 system, which are activated when the bacterial concentration is equal to or greater than 1×10⁷ CFU/mL, and indirectly or directly regulates the production and degradation, bacterial toxin secretion, and bacterial growth of the biofilm by regulating the corresponding genes. The research progress of the group effects of Staphylococcus aureus were reviewed, and a new direction for future research on Staphylococcus aureus was found. There is an inevitable relationship between the pathogenicity of Staphylococcus aureus and the formation of biofilms and the quorum sensing system of Staphylococcus aureus.

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Zyxin protein function in cells and its research progress

YUE Shi-chao, CAO Ya-qian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 13-17. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.003

Abstract （490）

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Zyxin protein is an important member of the focal adhesion protein complex, which is a highly dynamic multiprotein complex found in cells. It is capable of transmitting signals from the extracellular matrix to the nucleus. Zyxin protein has a broad proline-rich domain, a nuclear export signal and three copies of the LIM motif. Recent studies have shown that Zyxin protein not only functions in actin-related biological processes, but also plays an important role in cell migration, cell adhesion, cell growth, DNA damage repair, tumor growth, and natural immunity. The function of Zyxin protein mainly in cells and the latest research progress was described.

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Effects of plant nutrient concentration, dosage and spraying number of essential amino acid content in rice

LIU Xing-hai, TANG Jian-zhou, TANG Yu-mei, LI Chun-yan, ZHANG Zhi-yuan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 18-20. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.004

Abstract （494）

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Field experiments and laboratory analysis methods were used to study the effects of spraying different concentrations, dosages and times of plant nutrients on the essential amino acid content in white rice from 2017 to 2018. The results showed that the essential amino acid content of rice was increased from concentration of plant nutrients 8.0%~16.0%. The application of 11.0% plant nutrient was 61.875 kg/hm² per dose, the application in the field was 562.5 kg/hm² per dose. The essential amino acid content of white rice reached 3 310.6 mg/100 g,which was 16.73% higher than that of the control. The application of 16.0% plant nutrient was 30.000 kg/hm² per dose, the application in the field was 187.5 kg/hm² per dose. The essential amino acid content of white rice reached 3 156.6 mg/100 g,which was 10.96% higher than that of the control,but burns were produced by using this concentration on Xiangzaoxian 45. In order to save cost, reduce spraying amount and maximize the content of essential amino acids in rice, in practical application, the concentration of plant nutrients is 10%, the dosage of plant nutrients is 56.25 kg/hm² per dose, the dosage of plant nutrients solution is 562.5 kg/hm² per time in the field, and it is sprayed once at the end of booting and full heading stages.

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Effect of different planting density and nitrogen application on yield of Emai 195

DUAN Bao-quan, CHEN Bo, ZHANG Jun-hua, TAN Qing-feng, YANG Ze-fu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 21-24. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.005

Abstract （584）

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In order to accelerate the renewal of wheat varieties in Yicheng city and explore the yield performance of the new high quality medium gluten special wheat cultivars Emai 195 in Yicheng city, the orthogonal cultivation experiments of Emai 195 basic seedlings and nitrogen application two factors were carried out to study the optimum planting density and nitrogen application in Yicheng city. The results showed that under the condition of moderate fertility, when the basic seedlings were 255×10⁴ plants/hm² and the total nitrogen application was 165 kg/hm², the yield of Emai 195 in Yicheng city was the highest, which was the best fertilizer treatment.

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Investigation and analysis of crops germplasm resources in Jianshi county of Hubei province

LI San-he, LI Hou-qin, DAI Feng-ting, WAN Zheng-huang, YOU Ai-qing, JIAO Chun-hai

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 25-34. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.006

Abstract （655）

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In the third national general survey of crop germplasm resources, 254 crop germplasm resources including rice, corn and potato have been collected into the National Bank in Jianshi county of Hubei province. The changes of cultivated area and yield of main food crops, fruit trees, vegetables and cash crops in Jianshi county between 1956, 1981 and 2014 were analyzed. It was found that Jianshi county was rich in natural resources, and the local varieties of rice, wheat and rape reduced rapidly, and some local varieties even have nearly disappeared, but the other kinds of crops still preserved some local varieties and some minor crops were planted mainly with local varieties. The total planting area of food crops changed a little in these three investigations, and corn is the most important food crop in Jianshi all the time. The planting area of fruit trees and main cash crops increased rapidly, but the planting area of vegetables decreased seriously. 44 local resources were collected in the resource investigation. According to the abundant resources of Jianshi county, the weak agricultural infrastructure construction, and the low element of agricultural science and technology, some countermeasures were proposed, such as strengthening the protection and utilization of existing crop resources, accelerating the cultivation of new agricultural varieties and the extension and training of new technologies, strengthening agricultural technology service accelerating the construction of modern agricultural industrialization, increasing government support and propagation.

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Effects of microbial product and trace elements in pine needle/coconut coir substrate on seedling growth of Capsicum annuum L.

DOU Dan, ZHANG Yu, LUO Xue-gang, YANG Yang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 35-41. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.007

Abstract （860）

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In terms of Pine needle/coconut matrix is not fully decomposed and lacks nutrients and trace elements, which is insufficient to meet the growth requirements of seedlings of Capsicum annuum L.. The effects of different microbial agents and trace elements on the physiological characteristics, chlorophyll content and chlorophyll fluorescence characteristics of Capsicum annuum L. seedlings were studied by seedling tray test. The results showed that under the treatment of Lvlong 0.16 g/plant(A3) and Kehuangwei 0.08 g/plant(B2), the growth of Capsicum annuum L. seedlings was the best, and the physiological indexes were significantly improved compared with the control. The seedling index and root-shoot ratio increased by 49.66%, 11.37% and 44.30%, 10.87%, respectively. The chlorophyll content and PI_abs increased by 19.17%, 34.76% and 29.41%, 32.37%, respectively. The addition of Lvlong and Kehuangwei to the pine needle/coconut matrix could promote the decomposition of the matrix, which was beneficial to the germination of Capsicum annuum L. and supplement nutrition at the seedling stage. It had a great influence on the PSII system and promoted the physiological growth process such as photosynthesis of the seedlings.

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Effects of PEG simulated drought on the growth of fresh-eating Manihot esculenta Crantz SC9 seedlings

DUAN Yuan-jie, MENG Fu-xuan, YANG Yu-jiao, LIU Hai-gang, HUANG Jie, WEI Yun-xia

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 42-44. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.008

Abstract （488）

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Seedlings of fresh-eating cassava Manihot esculenta Crantz variety South China 9(SC9) were used as materials, different concentrations of PEG were used to study drought resistance under simulated drought stress. The results showed that with the increase of PEG concentration, the drought stress increased, the number of leaf abscission increased, plant height growth, crown area, dry weight of aboveground part, dry weight of leaf, dry weight of supporting structure, leaf area, leaf area, leaf area of SC9 seedlings increased. The fresh weight of the slices decreased gradually, the relative conductivity increased gradually, and the content of photosynthetic pigments increased first and then decreased. That SC9 could adapt to drought environment by changing morphological characteristics, growth indicators and physiological functions of leaves under drought stress, and had good tolerance to drought stress.

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Effects of nitrogen fertilizer coupled with uniconazole on photosynthetic characteristics of trunk fruit-shaped walnut

ZHOU Yan, YU Jun, ZHANG Rui, LI Qian, LI Xue, CHEN Xin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 45-50. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.009

Abstract （466）

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The trunk fruit-shaped walnut(Juglans regia L.) of Wen185 walnut was selected as the research material, and the coupled mode of nitrogen fertilizer with uniconazole with different concentration gradient was set to study the effect of nitrogen fertilizer on the photosynthetic characteristics of walnut fruits during the expansion stage. The results showed that the net photosynthetic rate(Pn) of walnut treated with nitrogenous fertilizer coupled with uniconazole was higher than that of the control group,among them,N 665.5 g/plant, uniconazole(S) 250 mg/L treatment reached the maximum, which was 21.17 μmol/(m²·s), an increase of 37.99%. Compared with the control group, intercellular carbon dioxide(Ci) change was relatively stable. The high nitrogen fertilizer treatment had a large Ci, and the high nitrogen treatment had great Ci value; Transpiration rate(Tr) and stomatal conductance had a large range of variation and the variation trend was consistent, both reached the maximum value in treatment N 665.5 g/plant, S 150 mg/L, and high concentration enizolium treatment had a certain inhibitory effect on transpiration rate(Tr) and stomatal conductance. The instantaneous water use rate(WUE) changed little with the treatment, which was promoted by low-concentration and inhibited by high-concentration on the whole. The main influencing factors of stomatal limiting value(Ls) was nitrogen fertilizer, and low nitrogen fertilizer promoted the improvement of Ls value to a certain extent, while high nitrogen fertilizer inhibited it. In addition, the coupling treatment of high nitrogen fertilizer and high uniconazole can increase the leaf saturated vapor pressure deficit(V_pdl). The combination treatment of 665.5 g/plant nitrogen fertilizer and 250 mg/L uniconazole improved the photosynthetic performance of trunk fruit-shaped walnut the best, which achieved the high yield and high quality cultivation of walnut.

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Effect of molybdenum fertilizer on peanut yield

WANG Li-qiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 51-53. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.010

Abstract （414）

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To verify the effects of trace element fertilizer molybdenum on peanut yield and the suitable amount a peanut field tests was conducted. The five fields were treated with different amount of molybdenum fertilizer at initial bloom stage and full bloom stage(each of 50%) on even base fertilizer.. The test index conclude the main stem height, lateral branch length, total branch number, fruit branch number, kernel weights per plant, 100 kernel weights, kernel percentage and yield. The results showed that the adding molybdenum fertilizer could increase the peanut yield by improvng the yield components such as the main stem height, branch length, total branch number, fruit length per plant, kernel weight, kernel yield, fruit number per plant. The best economical fertilization is 1.50 kg/hm².

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Immobilization effects of arsenic in soil by green synthesized iron nanoparticles

LI Liang, YANG Xiu-feng, ZHANG Zhong-hai

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 54-58. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.011

Abstract （420）

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In order to achieve efficient remediation of arsenic(As) contaminated soil, the suspension of iron nanoparticles (EL-nFe) was prepared by eucalyptus leaves extract. The sorption capacities of EL-nFe for As(III) and As(V) were investigated. Chemical methods were further used to study the immobilization effects of severe As-contaminated soil by EL-nFe. The results showed that the adsorption process of As(III) and As(V) by EL-nFe could be well described by the pseudo-first-order and Langmuir isothermal adsorption model. The maximum adsorption capacities of EL-nFe for As(III) and As(V) were 8.08 and 24.68 mg/g, respectively. The soil after application of EL-nFe significantly reduced the leachability and bioavailability of soil As. The extraction of As by SPLP and the extraction of bioavailable As by KH₂PO₄ decreased by 33.26%~52.42% and 6.57%~14.21%, respectively. At the same time, the result of soil sequential extraction showed that EL-nFe promoted the transformation of soil soluble As to the low bioavailability of residual As, which improved the degree of stabilization of As in the soil. Therefore, EL-nFe had a good immobilization effect on soil As and the cost was low, which can be used as a good passivation agent for severe As-contaminated soil.

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Effects of short-term submerged cultivation of Ipomoea aquatica Forsk on salinized soil in midsummer

PAN Rui-rui, QIAN Jia-yu, JIANG Jie-zeng, ZHANG Hao, ZHANG Yong-xian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 59-62. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.012

Abstract （410）

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In order to study the effects of short-term paddy and upland rotation on soil properties in midsummer high temperature season, taking Ipomoea aquatica Forsk as test material, a 40 d submerged cultivation test was carried out in the back-cover cultivation bucket of soil salinization treatment, and then 32 d of dry cultivation was used to explore its influence on the EC value, soil nutrient content and soil enzyme activity of 0~40 cm soil layer. The results showed that the water cut off after the short period of flooding in midsummer high temperature season, combined with the planting of fast-growing Ipomoea aquatica Forsk, it can obviously reduce the salinity of saline soil surface and equalize the nutrients in 0~40 cm soil layer. It is believed that the saline infiltration through short-term flooding, combined with the absorption and metastasis of fast-growing aquatic vegetables, it can effectively alleviate the soil salinization of the facilities caused by excessive use of chemical fertilizers.

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Risk assessment of wind disaster to greenhouse in Hebei province based on vulnerability curves

LI Ting, CHEN Xiao-juan, ZHANG Jing, SUN Yu-long, WEI Jun, LI Dan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 63-69. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.013

Abstract （373）

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Based on the disaster risk assessment theory and climate probability distribution model, the wind disaster risk to greenhouse in Hebei province was evaluated. The vulnerability curves of wind speed—solar greenhouse loss rate were constructed, considering disaster-causing factor and bearing body. The results showed that, with the increase of return periods, the strength and incidence of hazard factor and loss risk was greater. In terms of space, the southeastern area with low terrain had the highest hazard factor risk, mainly located in the eastern part of Cangzhou, as well as the northern Hengshui and eastern Handan. The extreme wind velocity was above 32.6 m/s. The basic wind pressure was consistent with the landforms’ distribution. The basic wind pressure was consistent with the distribution of the three landforms, with the high value occurring in the northwest plateau area and the plain area to the east of Taihang and the south of Yanshan Mountain areas. The vulnerability curve indicated that the threshold of wind speed which might cause loss to greenhouse was 20.0 m/s. The risk of greenhouse loss was affected by the combined effects of disaster-causing factor and bearing body. The risk in the northwest was generally lower than the southeast. The high-risk area was distributed in the junction of Tangshan-Qinhuangdao, western Tangshan, Langfang and south-central regions. The average loss was more than 500 000 yuan/km². The greenhouse area in the south-central part was smaller than that in the northeast, but the loss was higher owing to its higher hazard factor risk.

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Analysis of meteorological conditions on the pre-selected site of Jinan general airport in Jingzhou

HE Cheng-cheng, DENG Yan-jun, WANG Xiao-fang, CHEN Zheng-hong, YU Yu-zhi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 70-75. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.014

Abstract （620）

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Based on the ground meteorological observation data from 1955 to 2016 in Jingzhou Meteorological Station, the meteorological conditions in the preselected area of Jinan General Airport were statistically analyzed by means of linear trend, comparative analysis and other methods. The results show that pre-selected site area of Jinan General Airport has a low annual average wind speed, with an average monthly wind speed of 1.9~2.5 m/s. The annual static wind frequency is high, which is 18.2%, and the frequency of wind speed of 0~5.0 m/s is 89.99%. The airport pre-selected sites has few days with low visibility, the average days of the visibility, the annual average number of days with visibility less than 800 m, 800~2 000 m, 2 000~4 000 m is 9.8, 10.8,13.7 d, respectively. The average days of the torrential rain, strong winds, thunderstorms, hail, sand storms and other weather disasters is 3.30, 3.98, 32.20, 0.49, 0.02 d. The probability of high impact weather is lower than that of Wuhan Tianhe Airport. There are many favorable conditions and less unfavorable conditions in the pre-selection site. The influence of adverse conditions on flight can be avoided by improving the accuracy of prediction and the professional quality of management and operator.

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Selection of greenhouse whitefly [Trialeurodes vaporariorum (Westwood)] to different host vegetable

MU Xin, LIU Yuan-yuan, YANG Wei-nan, KONG Xiang-xin, JIN Yong-ling

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 76-80. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.015

Abstract （416）

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According to the method of indoor net cover catch insect to detect the selection index greenhous whitefly [Trialeurodes vaporariorum (Westwood)] to host plants, the number of distribution and the amount spawning of greenhouse whitefly on host plants, and measured the content of chlorophyll and leaf thickness of host plants. The results showed that the greenhouse whitefly had obvious selectivity difference to different host plants, greenhouse whitefly preference for the host were string beans, cowpea, cabbage, zucchini, tomato, no-string beans, cabbage without balls, rape, cucumber. The suitable host plants included pepper, Physalis pubescens, cherry radish, lettuce, okra, Dutch bean, endive, Shanghai green, mantang red radish, pea, color pepper, lettuce, chrysanthemum coronarium, spinach, Chinese cabbage, green amaranth with round leaves. The non-hobby host palnts were leeks, coriander and celery. Greenhouse whiteflies were more likely to land on the plant leaves with pubescence. The total of greenhouse whitefly was connected with the content of chlorophyll in the host plants, and no correlation with the thickness of host plant’s foliage.

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Occurrence regularity of Myzus persicae (Sulzer) in tobacco growing area of Liangshan state, Sichuan province

WU Xing-long, FENG Chang-chun, PU De-qiang, WANG Yong, LIU Dong-yang, LIU Hong-ling, CHEN Yu, WANG Xiao-qiang, LONG Gang, LI Chang-ming, JIANG Lian-qiang, LI Bin, GUO Shi-ping, LIU Yang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 81-84. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.016

Abstract （427）

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From 2017 to 2018, the occurrence of Myzus persicae in three tobacco growing counties in Liangshan state prefecture, Sichuan province was investigated with 5 point sampling method. The occurrence of Myzus persicae in three tobacco growing counties in Liangshan state prefecture has obvious temporal dynamics, but the annual difference is great. From 2017 to 2018, the occurrence of Myzus persicae in three major tobacco planting counties was a mixture of single-peak type and M type. The peak of Myzus persicae occurrence in the three counties in 2018 was 15~30 days earlier than that in the average year of 2017. It is found that temperature and rainfall are the main factors affecting the occurrence time and amount of Myzus persicae. Higher temperatures and earlier rainy seasons lead to earlier occurrence of peach Myzus persicae, therefore, the critical period of Myzus persicae control in tobacco growing areas of Liangshan state prefecture is from late May to late June. The specific time for prevention and control should be appropriately adjusted according to the current climate.

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Comparison test of anti-cracking varieties of medium-sized watermelon in southern facilities

GUO Huan-ru, ZHANG Hua-feng, ZHANG Lei-chen, YING Quan-sheng, HUANG Yun-ping, LIU Yan-bing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 85-87. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.017

Abstract （416）

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Medium watermelon ［Citrullus lanatus (Thunb.) Matsum. et Nakai］ varieties in South China had the disadvantages in easy cracking and poor transportation. Five varieties of watermelon with high cracking tolerance were introduced. The growth period, botanical character, fruit character, yield and disease resistance were compared. The results showed that Yongmi No.6 had anti-cracking,good quality,high yield and good disease resistance, which is suitable for the simplified cultivation mode of protected cultivation to meet the requirements of light and simplified cultivation of medium-sized watermelon in South China.

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Isolation and identification of three terrestrial orchids rhizosphere fungi

FAN Ji-hong, CHEN Lan-fen, TANG Jiu-yang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 88-90. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.018

Abstract （503）

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Three Chinese terrestrial orchids Cymbidium goeringii, Cymbidium tortisepalum Fukuy.var. longibracteatum and Cymbidium kanran were chosen to the samples for isolating and culturing rhizosphere fungi from substrates, which found and identificated three preponderant fungal strains during the storage, and which identified as Rhizopus oryaze, Chaetomium lobasum, Rhizopus icrosporus by 18SDNA sequences. It was found that the roots of orchid were mutualized with fungi to form rhizosphere structure, according to orchid roots stained and microscopical observed, so as to provide a theoretical basis for further research of orchids rhizosphere fungi.

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Study on growth rhythm and regression model of 29 years Eucalyptus cloeziana in Guangxi

MO Ji-you, YAN Li, WANG Jian-zhong, QIN Wu-ming, WEI Bing-jian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 91-97. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.019

Abstract （526）

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The Eucalyptus cloeziana of the Dongmen State Forest Farm of Guangxi was introduced from the 1980s. The diameter at breast height, tree height, volume and dry shape of the 29-years-old large inflorescence were studied, the results showed that the diameter at breast height, tree height and volume were 33.17 cm, 33.6 m and 0.92 m³, respectively, and the optimal model of diameter at breast height, tree height and volume growth regression of long-period Eucalyptus cloeziana were fitted out. By analyzing and comparing different large and medium-sized timber species with similar years of the Eucalyptus cloeziana, it is found that the growth of Eucalyptus cloeziana has obvious advantages, and even in different eucalyptus species, the Eucalyptus cloeziana also has the significant advantage in cultivation.

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Study on flavonoids from Ampelopsis grossedentata in Lichuan city

LI Yu, LIU Cui-jun, AI Lun-qiang, ZHANG Yu, LU Xin-peng, HE Mei-jun, RAN Ya-lan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 98-101. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.020

Abstract （384）

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The total flavonoids were extracted by 60% ethanol-ultrasound from the samples of Ampelopsis grossedentata collected from Qiyueshan mountain (1 500~1 600 m),Lichuan city, Hubei province. The total flavonoids in the young tips of Ampelopsis grossedentata was up to 453.67±11.26 mg/g detected by ultraviolet-visible spectrophotometer at the double-wavelength (λ=208.4 nm,λ=293.2 nm). Three compounds were purified from the organic extract using LC-HR-ESIMS,1D and 2D NMR spectral data analysis. Their structures were identified to be dihydromyricetin(1), myricetin(2),and taxifolin(3). The contents of dihydromyricetin, myricetin and taxifolin were 186.31±10.43, 13.83±1.32 and 14.76±2.26 mg/g respectively detected by HPLC.

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Preliminary study on shoots growth of newhall navel citrus by controlling water and fertilizer

LIU Jia-cheng, LIU Dong-hai, LIU Feng, LIU Yong-zhong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 102-104. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.021

Abstract （501）

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The newhall navel citrus (Citrus sinensis Osbeck ‘Newhall’) tree was used as the material. In the summer shoot growth period, controlling water and fertilizer was used as a treatment(T1), and the NPK water soluble fertilizer was applied by drip irrigation as control (CK). During the autumn shoots growth period, the controlling water and fertilizer treatment (T2) was applied to the trees which was applied by CK and T1 treatment in summer. Shooting rate, shooting density, shoot length, shoot thickness and leaf number of shoot were surveyed after the summer shoots and autumn shoots were matured, respectively. The results showed that T1 treatment significantly reduced the summer shoot shooting rate, summer shoot length, summer shoot thickness and leaf number of newhall navel orange. Compared with the CK+T2 treatment, the length of autumn shoots in T1+T2 treatment decreased significantly, and the proportion of short shoots in T1+T2 treatment increased, the growth of summer and autumn shoots was restricted to some extent. The results laid a foundation for the research and development of water controlling measures or fertilization frequency in the actual production process to achieve the labor-saving and effective regulation of citrus shoot growth.

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Research on diversity of macrofungi in Xujiaba of Jingdong county in Yunnan province

LIU Chao-mao, LI Ping, YANG Bin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 105-115. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.022

Abstract （384）

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Based on the investigation and identification of macrofungi in Xujiaba of Jingdong county in Yunnan province, the species diversity, dominant families and genera of macrofungi were analyzed. The results showed that the macrofungi in this area involve 2 phyla,ascomycota and basidiomycota. 210 species of wild macrofungi were found, belonging to 88 genera, 41 families, 13 orders and 6 classes. Among them 7 kinds of ascomycetes belong to 6 genera, 5 families and 203 kinds of basidiomycetes belong to 83 genus and 36 families. There were 6 dominant families, accounting for 14.63% of the total number of families. There were 9 dominant genera, accounting for 10.11% of the total number of families. According to their utilization value, edible fungi, medicinal fungi,medicinal food and bacteria and toxic fungi were classified, edible fungi had 75 species,accounting for 35.71% of the total, medicinal fungi had 22 species, accounting for 10.48% of the total, medicinal food and bacteria had 33 species, accounting for 15.71% of the total, toxic fungi had 30 species, accounting for 14.29% of the total, and there were 50 unknown species, accounting for 23.81% of the total. The area were rich in wild macrofungi、medicinal bacteria resources, that has great potential for exploitation and utilization.

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Study on continuous breeding of Hubei black-head goats in body weight and size

ZHANG Feng, ZHANG Nian, XIONG Qi, SUO Xiao-jun, TAO Hu, YANG Qian-ping, LI Xiao-feng, YANG Juan, CHEN Ming-xin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 116-120. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.023

Abstract （407）

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This study was aimed to statistically analyze the body weight and size(withers height, body oblique length, heart girth, cannon bone circumference) of Hubei black-head goats in three generations, so as to evaluate the effect of breeding. The results showed that through three generations of breeding, the core group of each generation of Hubei black-head goats has been improved. However, the increment varied from generation to generation, which basically showed that the growth rate was slow growth from zero generation to first generation, fastest growth from first generation to second generation, and slowest growth from second generation to third generation, coefficient of variation was relatively stable. After the third generation of breeding, the indexes of body weight and body size were basically stable. Compared with the body weight and size index of zero generation, that of third generation significantly increased at each month's age. The body weight average increment of ewes was 12.45%, and the body size increment was 4.37%. The weight increment of rams was 15.06% and the body size increment was 7.40%.

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Effect of different feeding ratio on late-stage production performance of egg seed

BIAN Hong-xing, HU Dao-jun, JINAG Hui-sheng, LI Fei-xiang, ZHI Wei-wei, WANG Wen-jian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 121-123. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.024

Abstract （360）

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The restricted feeding experiments for 360 days hen, the experiment was divided into 4 groups, respectively, the limit feeding 3%, 5%, 7% and control group. The results showed that there was no significant difference between the egg yield rate of the experimental group and the control group (P>0.05), and the average egg rate of the experimental group was 1.8% higher than that of the control combination of seed species, which was very significant(P<0.01). In the provision of qualified eggs, the limit of 3% and 5% of the group were better than the 7% group. The weight of egg planting becomed lighter with the more limited ingredients. 5% group was better than those of other groups.

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Effect of large seaweed, seagrass on marine ecological aquaculture and its application in marine ranching

WANG Yan, LYU Dong-wei, TIAN Yu, WANG Xue-ming

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 124-128. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.025

Abstract （752）

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The research on the symbiosis system of algae, seaweed and economic fishery crops at home and abroad in recent years were summarized. On this basis, according to the positive role of seaweed, seagrass in marine ecological aquaculture, and also combined with the current marine ranching situation in China, the further prospects for the practical application of algae, seaweed in marine ranching in China were put forward.

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Effects of changing to different diets on the growth and survival of Coreius guichenoti larvae

SHU Qi-lin, HUANG Jin, XU Xian, HU Ren-yun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 129-131. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.026

Abstract （416）

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The Coreius guichenoti larvae were raised with fairy shrimp, limnodrilus and particulate feed bait feed for 60 days to study the effects of the three different feeding method on the survival rate and growth rate. It was found that the larvae fed with limnodrilus had higher survival rate, total length, body length, weight than other groups after 30 days post hatching(dph), and the specific growth rate was(4.44±0.30)%/d. The total length, body length, weight and specific growth rate were the lowest of the particulate feed group after 15dph. The results indicated that different feeding method have a significant effect on the survival and growth of the larvae. Feeding the fairy shrimp before 30 dph, feeding the limnodrilus after 30 dph can effectively improve the survival rate and growth rate.

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Dynamic changes of trace elements in leaves and stemsin different feeding ramie

TANG Di-luo, WANG Hong-wu, XIONG Wei, ZHU Wei, TU Xiu-liang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 132-135. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.027

Abstract （394）

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This study used three different feeding ramie(Boehmeria nivea L.) as the research object. The dynamic changes of trace elements (Cu、Mn、Zn) in leaves and stems of 3 kinds of feeding ramie were studied and correlation between the content of trace elements in leaves and stems of feeding ramie was analyzed. The results showed that the dynamic changes of Cu, Mn and Zn contents in leaves and stems of 3 kinds of feeding ramie were inconsistent. The contents of Cu, Mn and Zn in different varieties of feeding ramie were higher than those in stems. The order of Cu, Mn and Zn in different varieties of feeding ramie leaves and stems was: Mn>Zn>Cu. There was a significant positive correlation between Mn in the stem of ramie and Mn in leaves (P<0.05).

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Analysis of carotenoids chlorophyⅡ and in cassava tissue culture seedlings

LUO Xiu-qin, WEI Zhuo-wen, YANG Long, XUE Jing-jing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 136-140. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.028

Abstract （377）

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Ultraviolet-visible spectrophotometer was used to detect the content of chlorophyⅡ and carotenoids in TM60444 and SC9 tissues culture seedings. Meanwhile, the relative genes of carotenoids biosynthesis were analyzed. The results showed that there is nearly no difference between two stains and the contents of chlorophyll and total carotene in two seedings: leaf>stem>root; and the content of carotenoids: leaf>stem, while the stem is approximately the root.

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Research on high precision positioning system of unmanned agricultural machinery

GONG Peng-cheng, TAN Ying, ZHAN Yun-feng, CHENG Hui-fen, LI Ling, DENG Zhang-hui, WANG Zi-qiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 141-146. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.029

Abstract （416）

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To realize the unmanned and high-precision positioning of agricultural machinery, a high precision positioning system of unmanned agricultural machinery was designed. The remote control center system based on Pixhawk flight controller, including sensor module, communication module, satellite positioning and other modules, and was mainly responsible for setting up the agricultural machinery work place, travel route and operation mode. The system was based on high-precision navigation technology, which enables the agricultural machinery to accurately and quickly reach the task point and work. Moreover, the NuttX was used in software design for operating the secondary development to improve the system’s extended functions.

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Research of the near wild cultivation monitoring system of Tin caulis dendrobii

MA Fei-hong, WU Jun, ZENG Song-wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 147-151. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.030

Abstract （461）

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In order to meet the industrial demand, a set of perfect monitoring system for the near wild cultivation of Tin caulis dendrobii was built. The monitoring system mainly used the precision sensing equipment and STM32 central processing chip to collect and process the temperature and humidity and CO₂ concentration data of dendrobium cultivation, and then regulated the spraying device; data interaction between terminal equipment and Internet was completed through GPRS module; finally, Mysql and Eclipse software were used to complete the interaction with the database and web development, realize remote monitoring function. In order to improve the effectiveness of cultivation environment humidity data and ensure the normal operation of sprinkler device, the system added the least square real-time prediction algorithm in the data processing process to identify abnormal or damaged humidity sensor.

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Development status, problems and countermeasures of geographical indications of agricultural products in central region: Analysis based on survey data based on Hubei, Hunan and Jiangxi provinces

WANG Dan, LI Yan-jun, LI Lin-zhu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 152-156. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.031

Abstract （486）

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As a special agricultural product brand, agricultural product geographical indications contain unique market competitive advantages and huge brand value, which can not only promote the development of regional economic development, but also become a business card to promote culture dissemination. The central region is an important agricultural product production area. The registered geographical indications of agricultural products have begun to take shape and present diversified characteristics. However, in the development process, there are problems of management confusion, long application time, high application cost, emphasis on application, light use and supervision, and insufficient publicity. To promote the better development of geographical indications for agricultural products in the central region, it is necessary to start from reforming the top-level design, improving the regulatory system, strengthening technical guidance, implementing cross-regional and cross-brand alliances, fostering industrial clusters, and increasing publicity efforts.

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A probe into the ways to improve the leadership of minority female leaders: Take Kazakh female leaders as an example

GULINIYAER·BIEKEXI, LIU Xiang-hui, ZHANG Shao-kai, WANG Bing-gang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 157-160. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.032

Abstract （505）

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According to the survey data of Kazak female leading cadres in Kazak Autonomous Prefecture of Ili, taking Kazakh women leading cadres as an example, the current situation, advantages of Kazakh women leading cadres, and factors affecting their leadership are analyzed, and the path of improving the leadership of Kazakh women leading cadres is proposed.

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Research on the dynamic evaluation system of enterprise access for the Three Gorges follow-up agriculture projects

LAI Hong-bing, LU Xing, WU Zhao-juan, ZHENG Gan-tian, TONG Zhu, HE Xiu-juan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 161-165. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.033

Abstract （451）

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Starting from the problems in the access conditions and evaluation methods of the Three Gorges follow-up agricultural projects, basing on the Interim Measures for the Implementation and Management of the Three Gorges Follow-up Work Plan, combining with the practical experience of other types of agricultural project enterprise access evaluation in China, the AHP Analytic Hierarchy Process is used to construct a dynamic evaluation system for the access of the Three Gorges follow-up agricultural projects, so as to improve project investment benefits.

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Problems and countermeasures of ecological agricultural project management in the Three Gorges Reservoir area

LAI Hong-bing, WU Zhao-juan, LU Xing, JIANG Fan, TONG Zhu, HE Xiu-juan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 166-169. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.034

Abstract （367）

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Based on the importance of the development of ecological agriculture in the Three Gorges Reservoir area, and based on the investigation and analysis of the implementation of ecological agriculture projects in the Three Gorges Reservoir area, the problems of inadequate preliminary argumentation in existing project management, complex procedures for application and approval, inadequate construction management system, low level of supporting fund protection, and incomplete operation management and protection system are identified. Countermeasures and suggestions such as updating project management concepts, improving management decision-making levels, strengthening project dynamic management, improving diversified investment mechanisms, improving project operation management and protection mechanisms, and establishing post-project evaluation systems are put forward.

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Analysis of influential factors of international competitiveness of tourism service trade in Henan province

GAO Li, ZHAO Shu-ya, WU Lei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 170-176. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.035

Abstract （489）

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Based on the relevant statistical data of Henan province from 1997 to 2016, a VAR model was established, and the impulse response function method and variance decomposition method were used to conduct an empirical analysis of the influential factors of Henan province's tourism service trade international competitiveness. The results show that the number of tourism majors, Internet users, and number of travel agencies in higher education institutions in Henan province can promote the international competitiveness of Henan's tourism service trade. It is suggested that Henan province optimize the employment structure, establish a tourism big data sharing platform, and promote the coordinated development of traditional travel agencies and OTAs to increase the international competitiveness of tourism service trade.

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The impact of external financing on enterprise innovation: Evidence from Zhongguancun firm panel data

GAO Qing-zhu, ZHANG Shi-mei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 177-183. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.036

Abstract （490）

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Technological innovation is inseparable from the support of the financial system, but what kind of financial structure is more conducive to technological innovation is an urgent problem. Using the panel data of Zhongguancun High-tech Enterprises from 2005 to 2015, the relationship between R&D investment and corporate innovation performance was tested. And the impact of credit financing and equity financing on the relationship was also analyzed. The results showed that R&D investment had a significant positive correlation with corporate innovation performance; credit financing positively regulates the relationship between R&D investment and innovation performance; and equity financing plays a negative role in regulating R&D investment and innovation performance. The deeply discussion on the inter-logic of high-tech enterprise innovation financing was helpful to understand the impact of different external financing channels on the relationship between R&D investment and innovation performance. It also provided empirical evidence and decision reference for decision makers to take reasonable financing channels and optimize financial resource allocation.

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Research and reflection on the implementation of the national key research and development program“Modern Food Processing and Grain Storage and Transportation Technology and Equipment”

WANG Li-wei, ZHANG Xing-zhong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 184-186. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.037

Abstract （376）

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National key research and development program "Modern Food Processing and Grain Storage and Transportation Technology and Equipment" special organization implementation, analyzed the special project project led by unit area, propertied and distribution of funds, put forward about participating unit quantity limitation, academy of agricultural sciences system in the role of specific implementation, as well as national key new product development plan design thinking, provided the basis for the special project implementation and management by objectives.

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Research on rural project operation from the perspective of “Process-Event” analysis: Take Xingcun as an example

LIU Qian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 187-190. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.038

Abstract （399）

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From the perspective of “process-event” analysis, this paper analyzes the operation process and results of Xingcun project, and the results showed that the current rural self-supervision mechanism was lacking, the endogenous development power of villages was insufficient, and the national technical governance was difficult to be effective.

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SWOT analysis of the development of rural time banks in Nanjing: Taking zhu town in Liuhe district as an example

GUO Jian-ping, PU Xiao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 191-195. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.039

Abstract （472）

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Based on the actual situation of Zhuzhen in Liuhe district, Nanjing, this paper explored the advantages and limitations of developing time banking policies in rural areas through SWOT analysis, and analyzed the opportunities and challenges brought by agricultural modernization and marketization for time banks. Based on SWOT analysis, it is proposed to strengthen government supervision to improve credibility, improve the establishment of social organization system, and explore the time-localized operation mode of the bank to achieve the purpose of benign development of rural elderly resources and the development of old-age services in rural areas.

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Difficulties and solutions to inter-governmental cooperation in rural public safety prevention

GUO Shuai-shuai, ZHENG Ting-yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (4): 196-201. DOI: 10.14088/j.cnki.issn0439-8114.2020.04.040

Abstract （396）

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Through a review of inter-governmental collaboration theory and public safety research, it is found that there are problems such as complex risk sources, difficult resource allocation, weak collaboration motivation, and not outstanding effects in the governance of inter-governmental collaboration in rural public safety. The optimization path of inter-government collaboration in security governance should set the principles of collaborative governance while establishing the basic principles, and create a new situation in rural public security governance by creating a cross-domain governance cooperation mechanism.

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Research progress infingerprint and bioactive components of Dendrobium officinal Kimura et Migo

GUO Lei, XIAO Kun-min, XU Yang, TAN Da-cong, WANG Jun-min, HUA Yan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 5-9. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.001

Abstract （619）

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： Dendrobium officinal Kimura et Migo has both high edible and medicinal value, which not only contains essential amino acids for human body, but also contains polysaccharides, flavonoids, alkaloids and other bioactive components, these ingredients can reduce blood pressure, blood sugar, anti-tumor and so on. However, it is difficult to identify Dendrobium officinal Kimura et Migo,because of its geographical origin and complex chemical components, use fingerprint technology could provide some theoretical basis to identify and evaluate Dendrobium officinal Kimura et Migo. In addition, domestic and foreign scholars had been isolated and identified polysaccharides, flavonoids, alkaloids and other bioactive components, the effect of these bioactive components was confirmed and their physiological mechanism was deduced. The relevant research on bioactive components and its function mechanism of Dendrobium officinal Kimura et Migo in recent years were summarized,in order to provide some scientific basis for identifying and drug developing of Dendrobium officinal Kimura et Migo.

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Research progress,hot points and inspiration of healthy tourism based on text analysis

CHANG Xue-ping, HOU Jun-dong, ZHUANG Xiao-li, RUAN Xin-xin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 10-16. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.002

Abstract （536）

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： With the aggravation of aged population,the society's attention to health is increasing. Tourists hope to strengthen their health when traveling, so healthy tourism market has been expanding, and healthy tourism has become the focus of scholars at home and abroad. The literature measurement method, text analysis method and social network analysis method were used to sort out the relevant research results, and the inscriptions and keywords of relevant literature on the research of healthy tourism at home and abroad were analyzed. Four research hotspots were obtained, including research on healthy tourism resources and product development, research on development countermeasures and trends of healthy tourism, research on medical and healthy tourism, research on forest healthy tourism. The research progress of healthy tourism at home and abroad were summarized, and the future research direction of healthy tourism in China was prospected.

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Breeding and application of new maize variety Hengyu 828 with high-quality and density-resistant

FANG Yong, HE Zhang-fei, WANG Hai, YU Qin-zhi, ZHOU Yu-ming, LI Yuan-xin, LONG Xing-xiang, ZHU Dong-fu, LI Wei-rong, HUANG Chun-yu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 17-19. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.003

Abstract （547）

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： Hengyu 828 is a commercial maize（Zea mays L.） variety with the good comprehensive characters and good appearance,high quality,density-tolerant and high yield.It is a hybrid between female parent HS892 and male parent HS2915 which were bred by Guangxi Hengmao Agricultural Technology Co., Ltd.. It combines traditional breeding methods and modern breeding technology. The ear is cylinder-shaped type, 17.4 cm length and 5.0 cm in diameter. The grain is yellow and flint type. The hundred-grain weight is 32.8 g. The average fresh ear yield of spring is about 8 500 kg/hm² and the yield of autumn is about 8 400 kg/hm². It takes averagely 112 days in spring and 100 days in autumn from sowing to harvest. It is suitable for planting in Guangxi region.

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Effects of density, fertility on the bolt yield of Brassica napus L

MA Meng-ya, ZHANG Ya-tao, YAN Guan-bo, HUANG Cheng, MA Chao-zhi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 20-23. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.004

Abstract （457）

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In order to explore the suitable cultivation methods for oil and vegetable dual-use rape（Brassica napus L.） varieties， and screen out early-ripening rape varieties suitable for cultivation in Hubei province， taking 19 cabbage-type rape varieties， 1 Chinese cabbage variety， and 1 Chinese cabbage variety as materials， the effects of density and fertility on the yield of rape cultivars were studied. The experiment set high density and low fertility （transplanting density of 108，000 plants/hm2， no external application of compound fertilizer）， high density and high fertility （transplanting density of 108，000 plants/hm2， adding 1 additional compound fertilizer before pumping， the application rate is about 600 kg/hm2） and low density and high fertility （the transplanting density is 54，000 plants/hm2， and the compound fertilizer is added once before pumping， and the application rate is about 300 kg/hm2）. The results showed that among the 21 varieties， compared with low density， under high density condition， 4 varieties increased highly significantly， 8 varieties increased significantly， 6 varieties did not increase significantly， and 3 varieties reduced yield； Compared with low fertility， the yield increase of 9 varieties was significant or extremely significant， and the yield reduction of 7 varieties was significant under high fertility condition；the average yield of 6 late-ripening rapeseed varieties increased by 61.21% under high density condition compared with low density， and increased by 49.50% under high fertility conditions compared with low fertility； the average yield of pupae of 13 early-maturing varieties increased by 31.53% under high density condition compared with low density， but there was no significant change between low fertility and high fertility conditions. It can be seen that increasing planting density helps to increase the yield of rape cultivars. The fertility affects the yield of rape cultivars varies from species to species.

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Effects of different stubble height on the growth and development dynamics,yield of Fengliangyouxiang No.1 ratooning rice

LEI Zhi-xiang, CHEN Chuan-an, PENG Jun-peng, YUAN Gao-hua, HU Jiong, XIAO Sen, SUN Fan, LIU Dao-hong, XIONG Lu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 24-27. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.005

Abstract （446）

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： In order to explore the appropriate stubble height of ratooning rice in Tianmen city, Hubei province, taking the Fengliangyouxiang No.1 as experimental materials, the effects of different stubble height on the growth and development dynamics, yield of ratooning rice were studied. The results showed that, as the stubble height of Fengliangyouxiang No.1 increased at the range of 15~45 cm, the growth period shortened, the plant height increased, the ratooning tillers increased, panicle length and grain number per panicle all decreased, effective panicles, and ratooning rate increased, the stubble height of 45 cm showed the highest yield, was 4 570.45 kg/hm². Comprehensive analysis of the growth period, ratooning rate and yield,the highest stubble height should be reserved for ratooning cultivation of Fengliangyouxiang No.1.

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Research on classification and fusion scale of sentinel-1A and sentinel-2A with different cloud cover

LUO Dong, LUO Hong-xia, LIU Guang-peng, LEI Xi, FENG Hua-mei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 28-36. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.006

Abstract （501）

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： This research aimed to improve the utilization ratio of optical remote sensing image in cloudy areas and explore the best fusion scales of different cloud cover optical remote sensing image and SAR image. Taking the Yuzhong area of Chongqing city as the research area, wavelet transform fusion, multiplicative transform fusion and high-pass filter fusion was carried out based on sentinel-1A polarized synthetic aperture radar （SAR） image and different cloud cover of sentinel-2A （cloud volume of 0, 10%, 20%, 30%） multi-spectral images, then the effect of image fusion was evaluated by image evaluation method, finally all images were classified by object-oriented methods,and the final classification accuracy was compared by the confusion matrix. The results show that, in the cloudless case, the wavelet transform has the best fusion effect, and the brightness and contrast of the original multi-spectral image are preserved to the maximum extent, which effectively prevents the loss of image information, and the interpretation ability of the vegetation is obviously improved, but after the fusion the image fidelity is poor, and the other two fusion effects are relatively inferior. Therefore, when multi-source remote sensing data fusion is performed in a cloudy fog region, especially when heterogeneous data is fused, the wavelet fusion algorithm is preferred. When the cloud cover is more than 10%, although the information entropy is slightly increased in the four fusion algorithms, the average gradient and standard deviation are reduced, which makes the interpretation difficult, resulting in the final classification accuracy is slightly lower than the sentinel-2A image, much lower than sentinel-1A images basically cannot meet the requirements for use. Therefore, when performing surface overlay interpretation, it is recommended to replace the optical images with SAR images.

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Analysis on variation of sunshine duration and its influencing factors in Huanggang city in recent 55 years

GUO Li, YAN Kai-lin, GONG Nian-zu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 37-43. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.007

Abstract （508）

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： In order to explore the variation rule and the reason of sunshine duration in Huanggang city, Hubei province, under the background of global climate change in the past 55 years, based on the daily sunshine data of nine national meteorological stations in Huanggang city from 1961 to 2015, the sunshine duration variation characteristics in Huanggang city were analyzed by using the linear trend estimation, Mann-Kendall parametric test, 5 years moving ayerage method and wavelet analysis. The results showed that, in the past 55 years, the annual sunshine duration in Huanggang city showed an obvious reduction trend, and the climate tendency rate was -41.576 h/10 a. The decrease of sunshine duration in summer was the most significant and the decrease of sunshine duration was the largest. The monthly sunshine duration in Huanggang city was the highest in August, while the lowest was in February, and the highest sunshine duration was 2.2 times of the lowest one. In August, the decrease of the sunshine duration was the most significant, followed by June and July. The annual sunshine duration of Huanggang city was abruptly changed in 1972, and the mutation was significant. In the past 55 years, there were obvious interannual and interdecadal variations of sunshine duration in Huanggang city, the sunshine duration was mainly controlled by three cycles. In terms of impact factors, the precipitation and vapor pressure were negatively correlated with sunshine duration, which was one of the reasons for the decline of sunshine duration. Although, the amount of total cloud and low cloud had a certain effect on sunshine duration, but not the main cause of sunshine declined.

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Development and utilization management of shallow ground water resources in Jiangyan district

ZHAO Min, CHEN Jing, ZHOU Hua, YI Peng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 44-48. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.008

Abstract （399）

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： Since the lack of unified planning, continuous expanding development and utilization of shallow groundwater might resulted partial imbalance in Jiangyan district. Based on the hydrogeological conditions and the parameters from pumping tests, a mathematical model of hydrogeologic in Jiangyan district was established by using Visual MODFLOW software. After continous adjustion of the parameters, the model was proved to be reasonable and effective for its good fitness on water level simulating. It can truly reflect the local hydrogeological conditions, and could be used to predict the shallow the groundwater level in Jiangyan district, and further predict the exploitable amount in future 10 years with the water level as control index. The results showed that the exploitable amount of shallow groundwater in Jiangyan district is 4.670 4 million m³/a till 2030 if current exploitation conditions unchanged. Compared with the actual exploitation situation, the exploitation and utilization potential of shallow groundwater in the study area is 76.41, which was far greater than 1.2. It indicated that under the current mining conditions, the shallow groundwater in the study area still has a certain mining space. Therefore, it is necessary to guide the development and utilization on the shallow groundwater in the study area in strict accordance with the distribution map of the remained shallow groundwater, and arrange the wells scientifically and reasonably at the potential areas.

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Analysis of groundwater chemical characteristics and formation causes of reservoirs in Guangxi based on SPSS

LIU Guo-zi, YANG Feng-gen, XU Bing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 49-53. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.009

Abstract （433）

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： The multi-statistical analysis software was used to analyze the chemical characteristics and formation causes of the groundwater in the reservoir area of Guangxi. Through correlation analysis, factor analysis and cluster analysis, it was found that the groundwater components were highly correlated and the dependence was high. The chemical types of water in factor 1, factor 2, and factor 3 were bicarbonate-chloride-calcium type water, sulfuric acid-magnesium potassium type water, and sulfuric acid-potassium sodium type water, which were basically consistent with the Shukalev classification. The karstification （dolomite, limestone, rock salt dissolution）, cation exchange adsorption and gypsum leaching of the study area had certain influence on the chemical characteristics of groundwater. The chemical characteristics of groundwater showed obvious spatial and temporal differences, and the dominant group of groundwater in different locations were different.

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Spatial distribution characteristics and impact factors of eutrophication in Jingpo lake

LIU Yu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 54-56. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.010

Abstract （344）

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： Based on the chlorophyll a concentration and synchronous Landsat 8 OLI data of Jingpo lake in September 2015, a combined band model of chlorophyll a concentration was established. According to the principle of goodness of fit and validation accuracy, the optimal inversion model was determined as（B₄+B₅）/B₃. The results showed that the spatial distribution of eutrophication in Jingpo lake is different by Carlson index method. Among them, most parts of the lake area belonged to the state of medium nutrition, the south part of the lake was in the state of poor nutrition, and the lake area near Jingbo township was in the state of eutrophication.

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Response of nitrogen uptake and root traits of rapeseed to the low nitrogen stress

CHEN Zhuo, HU Yi-fan, HAN Yong-liang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 57-62. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.011

Abstract （383）

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： In order to explore the response of nitrogen （N） uptake and root traits of rapeseed （Brasscia napus L.） to the low N stress, two conventional rapeseed varieties, “814” and Xiangyou15, with hydroponics cultured, were used as experimental material, and three nitrogen treatments 10, 3 and 1 mmol/L NO₃^- were set up with 10 mmol/L as control. The amount of N absorbed and its N-uptake efficiency of repseed were determined as well as the root-shoot ratio, root volume, root absorbing area, root diameter, the whole root length and the branching number were assayed. The correlation between the N-uptake efficiency and root morphological characters were analyzed. Moreover, the root activity and the activity of H⁺-ATPase in root plasma membrane were measured. The results showed that the amount of N absorbed by rapeseed was decreased, but the N-uptake efficiency increased under low-N stress. The roots of rapeseed were elongated and showed more branches with lower root volume and higher root absorbing area in the low-N treatments compared with the control. The root activities showed no significant difference between the treatment with control and the treatment with 3 mmol/L NO₃^- supplied, however, the root activity showed significantly decreased with 1 mmol/L NO₃^- supplied. Compared with normal N supplied, the activities of H⁺-ATPase in root plasma membrane of rapeseed were significantly increased under low N stress. It showed significant difference on the H⁺-ATPase activity of rapeseed among the treatments with 10 mmol/L, 3 mmol/L and 1mmol/L N supplied.

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Effects of biogas manure combined with chemical fertilizer on spring maize growth traits

LIU De-yao, ZHAO Jin-xi, LIU Xiao, WANG Xue-fen, HAN Shou-liang, SUN Hui, LIU Xin-cheng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 63-66. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.012

Abstract （365）

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： The purpose of this experiment is to study the effects of combined application of biogas manure and chemical fertilizer in different proportions on the growth traits of spring maize （Zea mays L.）. Spring maize variety Jinhai No.5 was selected as experimental material and 6 different fertilizer treatments were set up, labelled as CK（no fertilizer）, ZH20（20% biogas manure+80% chemical fertilizer）, ZH50（50% biogas manure+50% chemical fertilizer）, ZH80（80% biogas manure+20% chemical fertilizer）, ZF100（100% biogas manure）. The nitrogen application of the 5 fertilization treatments were all 352.5 kg/hm². Filed trails were conducted in randomized block design to study the effects on leaf development, plant height, earing traits and photosynthesis in five key growth stages （jointing stage, small bell-mouthing stage, big bell-mouthing stage, tasseling and silking stage and physiological maturity）. The results showed the application of 100% biogas promoted leaf growth most obviously at jointing stage and big bell-mouthing stage; Before big bell-mouth stage, the treatments with higher proportion of chemical fertilizer significantly improved the contents of chlorophyll, and there was no significant difference after big bell-mouth stage.

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Study on technology of accelerating decomposition on maize straw in semiarid area

LIU Fang-ming, DOU Jin-gang, GAO Yu-shan, SUN Yun-yun, HOU Zhong-hua, LIU Hui-tao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 67-71. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.013

Abstract （406）

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： This experiment studied the kinds of straw decomposing agents and the applying amounts of urea and organic fertilizer on the decomposition ration, decomposition rate and maize yield with nylon mesh bag method, in order to improve the technology of returning straw in semi-arid and construct the returning straw model of accelerating decomposition suitable for local production conductions. The results showed that the proper applying amount of urea 75 kg/hm² and organic fertilizer 45 t/hm² could significantly promote straw decomposition ration and decomposition rate（P<0.05）, and its maize yield increased 11.84% （P<0.05）. The straw decomposing agent from Jilin Academy of Agricultural Sciences had better effects, accelerated decomposition （P<0.05） and its maize yield could significantly increase 1.34% （P>0.05）.

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Basic research on application of microbial composite agents for rapid volume and weight reduction of heavy metal hyperaccumulator plants

LIANG Zhu-ming, LUO Xue-gang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 72-77. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.014

Abstract （387）

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： In order to solve the key technical problems of quickly process large quantities of hyperaccumulator plant bodies at low cost after environmental bioremediation,a composite agent consisting of Bacillus subtilis and Bacillus licheniformis, and plants such as Indian mustard （Brassica juncea） with strong enrichment ability for heavy metals were used in fundamental research of reducing the volume and weight of hyperaccumulator plants. The experiments result showed that the volume-reducing rate of hyperaccumulator plants treated with microbial vomposite agents reached 96% with 23 percentage point more than the control group. And the degradation rates of cellulose, hemicellulose and lignin reached 69.06%, 80.75% and 28.59%,with 27.35,14.75,5.49 percentage point more than the control group. The experimental results show that the microbial composite agents have significant volume reduction and weight reduction abilities for heavy metal hyperaccumulators plants and potential application prospects.

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Effects of fermentation factor on the decomposing rate and lignocellulose degradation during fermentation of wolfberry branches substrate

FENG Hai-ping, LI Xin, XIE Hua, YANG Dong-yan, PEI Hong-xia

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 78-83. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.015

Abstract （386）

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： The characteristics of the lignocellulose degradation were studied during the fermentation of wolfberry branches substrate. By design of orthogonal experiment, wolfberry powder branches and sophora alopecuroides stem powder mixed in ratio of 4∶1 were used to study the influence of fermentative factors on fermentation rotten rate and lignocellulose during the fermentation of wolfberry branches. The results showed that, by the end of the fermentation, the treatment, which the pile body is more branches, the decomposing rate is higher, accelerate the decay of the Chinese wolfberry powder branches on turns heap temperature of 60 ℃, water of 60%,added oil cake and inoculated with coarse cellulose degrading bacteria, promoted the decomposing and pile body, and can shorten the time of wolfberry rotten branches. Temperature, water, nitrogen and exogenous microorganisms had significant or extremely significant effect on the degradation of lignocellulose. Degree of lignocellulose degradation was shown as following order： hemicellulose, cellulose, lignin, the degradation rates of hemicellulose and lignin stayed above 20% and 11%. The treatment, which turns heap temperature of 60 ℃, water of 60%, added oil cake and inoculated with coarse cellulose degrading bacteria,the higher degradation rates of lignocellulose were got, resulting in promoting more effective degradation on organic matter.

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Introduction and screening of new facilities autumn varieties in Wuhan city

SUN Xiong-jun, CHEN Hong, DENG Xiao-hui, HE Shi-yu, BAO Xi-feng, REN Zhi-yong, YOU Ze-shuang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 84-87. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.016

Abstract （399）

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： Based on the botanical characters, resistance, yield and molecular markers of eight tomato varieties, new tomato varieties suitable for facility cultivation in autumn in Wuhan were screened. The results showed that the two varieties, Ansadi and Dida, had high yields and were resistant to yellow leaf curl virus disease（Ty-1）, root-knot nematode disease and tobacco mosaic virus disease. The fruit has good commercial properties and meets the consumption habits of Wuhan, thus can be promoted in Wuhan and surrounding areas. According to molecular marker detection, Maiami contains the Ty-2 gene.

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Analysis of amino acid metabolism in tobacco leaves among different varieties

LI Hong-chen, ZHU Shun-cheng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 88-91. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.017

Abstract （455）

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： To screen high-quality flue-cured tobacco varieties and clarify the differences in amino acid metabolism among flue-cured tobacco varieties, the dynamic changes of proline, glutamic acid and aspartic acid metabolism key enzyme activities and amino acid content during the tobacco leaf growth of Yuyan 10, NC89 and Zhongyan 100 were studied. The results showed that at 20 days of leaf age, the activities of Δ1-pyrroline-5-carboxylic acid synthetase, ornithine aminotransferase, proline dehydrogenase, aspartate aminotransferase and aspartate kinase all increased first and then decreased, the peak is at leaf age 30~40 d, Yuyan 10 had the highest enzyme activity; the content of proline in tobacco leaves increased continuously; after 30 days of leaf age, the content of proline in Yuyan 10 changed to highest. The content of free amino acids increased first, then decreased and then increased, and Yuyan 10 was the highest at each stage of leaf age.

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Effects of ryegrass overturning on the agronomic,photosynthetic physiological and economic traits of flue-cured tobacco

LI Heng-quan, LI Jin-qiu, MA Ming-hong, ZHANG Xue-ying, YANG Xin-yu, WANG Jing-ying

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 92-95. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.018

Abstract （394）

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： Tobacco variety of 'Longjiang 911' was used as experimental materials, a field experiment was conducted to study the effect of returning ryegrass on agronomic trait, chlorophyll content, photosynthetic characteristics and economic character of flue-cured tobacco. The results showed that the overturning pressure of ryegrass could promote the growth of plant height. The plant height was increased by 21.89%, 14.75% and 10.85% compared with the control（CK2）. The agronomic character and photosynthetic characters were improved, and increased the yield and output value. The output value increased by 5.27%, 24.89% and 2.13% compared with the control （CK2）, thereby improved the economic character of flue-cured tobacco. The agronomic character and economic character of the second batch of flue-cured tobacco were relatively good. Photosynthetic characters are the best for the first batch of conventional fertilization（T1）. There were no significant differences in conventional fertilizers （T1） and reduced fertilizers （T2）, indicating that ryegrass as a green manure may partially replace chemical fertilizers.

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Effects of different cultivation models on yield and quality of clematis

SHI Man-rong, LUO Yi-fa

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 96-100. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.019

Abstract （413）

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： In order to optimize the artificial cultivation techniques for clematis（Anoectochilus roxburghii）, orthogonal design experiments were used to study the effects of different cultivation substrates, different densities, and different fertilizers on the yield and effective components of A. roxburghii. The results showed that the cultivation substrate, planting density, and fertilizer had very significant effects on A. roxburghii polysaccharides, amino acids, and vitamin C; the cultivation substrate and planting density had significant effects on ash content; and the fertilizer had significant effects on Ca content. Synthesizing the effects of three factors on the effective components and economic yield of clematis, taking the cultivation substrate as peat soil∶coconut bran∶sand=4∶2∶1, cultivation density 3 cm×3 cm, and fertilizer management with No. 2 Feihuabao No.5 1 000 times solution+amino acid 1 000 times solution can promote economic benefits and quality improvement of clematis.

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Response of seed germination of Monarda didyma to environmental factors

LIU Na

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 101-105. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.020

Abstract （461）

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： A multi-factor experimental design was used to explore the effects of different environmental conditions such as light, temperature, moisture, salt, plant hormones and heat shock on the germination characteristics of American mint （Monarda didyma） seeds. The results showed that light and gibberellin can significantly promote the germination of American mint seeds, which can shorten the germination time of seeds and increase the germination rate of seeds. Seeds treated with 200 mg/L gibberellin had the highest germination rate of 87.78%. Heat shock treatment shortened the germination time of American mint seeds, and the highest germination rate was at 28 ℃ for 24 h. The best temperature for seed germination is 23~25 ℃, 25 ℃ is conducive to seedling growth. The seed germination rate, germination index and vitality index decreased significantly with the increase of water stress and salt stress. When the concentration of polyethylene glycol （PEG） is 0.15 g/mL, the germination rate is significantly lower than that of the control, and the seeds cannot germinate at 0.20 g/mL. The germination rate of NaCl concentration of 50 mmol/L was significantly lower than that of the control, and the seeds could not germinate at 400 mmol/L.

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Effects of pruning intensity and number of remaining buds on second flowering of Lagerstroemia indica

SHA Fei, YANG Hai-niu, LIU Fang, YE Yao-mei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 106-109. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.021

Abstract （390）

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： The five-year potted seedlings of Japanese multicolored （Lagerstroemia indica） were used as test materials. The effects of pruning intensity and the number of remaining buds on the secondary flowering of L. indica were studied using a two-factor completely randomized block design. The results showed that pruning intensity had a significant effect on sprout length, and the germination of light and medium-sized cutting shoots was better. The pruning intensity and the number of remaining buds and their combinations have extremely significant effects on the flower branch length, inflorescence length, and the number of inflorescences of the L. indica after pruning. The index of inflorescence length, number of small flower branches, and inflorescence number of the whole plant were slightly higher with slight cutting and moderate cutting with 4 buds respectively. Pruning intensity has a greater effect on the main phenological stages such as the initial flowering stage, the flowering stage, and the last flowering stage after prunes. L. indica can be re-flowered by trimming, and the flowering period can be prolonged by 55 d. Different pruning intensities and the number of remaining buds can effectively affect the condition of flowering again of L. indica.

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Effect of low potassium stress on defensive enzyme activity of Tagetes patula

WHANG Hong-yu, ZHANG Yan-li, ZONG Xian-chun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 110-112. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.022

Abstract （315）

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： Hydroponic methods were used to set five different potassium supply levels of 0.32,0.22,0.12,0.02,and 0.00 g/L to study the response of defensive enzymes of peacockgrass （Tagetes patula L.） to low potassium stress. The results showed that with the gradual decrease of potassium concentration, the activities of POD, SOD, and CAT of peacockgrass increased gradually; MDA content increased sharply; root plasma membrane permeability increased with decreasing potassium concentration. It was concluded that the defense enzyme activity of peacockgrass gradually increased under low potassium stress.

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Ant community composition of southeastern Yunnan

ZHU Hui-qin, XU Zheng-hui, HE Yu-cheng, HE Juan, ZHANG Peng, WANG Liang, ZHANG Yang, LIU Ping

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 113-120. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.023

Abstract （395）

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： In order to reveal ant diversity regularity in southeastern Yunnan, ant community composition of the area was surveyed through sample-plot method. Totally 10 subfamilies, 64 genera and 197 species of Formicidae were recognized. One dominant species（Pseudolasius sp.1, occupying 0.51%）, 2 common species（occupying 1.02%）, 18 relatively common species （occupying 9.14%）, 38 relatively rare species（occupying 19.29%）, and 138 rare species（occupying 70.05%） are found in the ant community of southeastern Yunnan. The richness of rare species helps to increase the value of biodiversity conservation in the area. Among the 5 surveyed vertical zones, their ant community composition has expressed obvious differences. The vertical zones of Shizong and Qiubei-Shizong have relatively higher average elevation, less vegetation types and simple tree species, therefore poor ant species are found in the community. In contrast, the vertical zones of Xichou-Malipo and Maguan-Hekou have relatively lower average elevation, rich vegetation types and complex tree species, therefore plentiful ant species are found in the community. It provides good conditions for the survival of ants, and the ant community is rich in species. A number of undetermined ant species are found in area, which indicates that a taxonomic work is necessary.

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GC-MS analysis of chemical constituents of volatile oil from different Thunber giaerecta of Yao medicine of ravea graffiti in Guangxi

ZHANG Xun, HE Qi-fa, QIN Shuang, YANG Hu-qiang, ZHOU Yi-hang, HUANG Jian-jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 121-123. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.024

Abstract （378）

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： To analyze the chemical constituents of the essential oil of Yao medicine from different producing Thunbergia grandiflora. The results showed that 49 chromatographic peaks were isolated from shitun, Bailong beach, Mashan and 24 chemical constituents were identified, accounting for 88.78% of the total volatile oil. 49 chromatographic peaks were isolated from Hongdu village, Chengjiang town, Duan county, and 30 chemical constituents were identified, accounting for 88.38% of the total volatile oil. 48 chromatographic peaks were isolated from Longwan township, Duan county, and 25 chemical constituents were identified, accounting for 80.01% of the total volatile oil. Nine of the chemical constituents of the volatile oil from the three places of origin are common to the three. It can be seen that the main components of volatile oil from different producing areas are different.

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Product quality evaluation of citrus peel pu’er tea in Pubei county,Guangxi

HE Qiu-yun, FENG Xiao-bin, LIU Yue-dong, HE Min, CHEN Xu-yu, YANY Li-wen, LI Qin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 124-128. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.025

Abstract （428）

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： Pysical and chemical indexes,heavy metals,pesticide residues and microbe of citrus peel pu’er tea in Pubei county, Guangxi of different manufacturers were were analyzed and evaluation by standards requirement. The results shows that moisture content were 7.25~11.50 g/100 g,ash content were 5.2%~7.9%, water extract content were 26.4%~33.4%,tea polyphenol content were 5.1%~13.1%; lead content were 0~0.079 mg/kg,arsenic content were 0~0.062 mg/kg, cad-mium content were 0.015~0.034 mg/kg, chromium content were 0.73~1.02 mg/kg, copper content were 8.65~15.70 mg/kg; 5 （HCH、bifenthrin,endosulfan,profenofos,chlorpyrifos） within the 16 pesticide residues haved detected in small amount of samples,microbial indicators： total colonies, coliforms and fungi were detected in different quantities, all detected results were under corresponding standard limits. Other pesticide residues and pathogens（salmonella, shigella and staphylococcus aureus） in all samples were not founded. Research shows, the physical and chemical indexes,heavy metals,pesticide residues and microbe of citrus peel pu’er tea in Pubei county, Guangxi were in line with the standards and its products were safe and reliable.

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Design and implementation of meteorological assistant decision system based on C/B/S

QIU Zhong-yang, WU Jing-lu, LIU Wen-wei, CHEN Hong-bo

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 129-137. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.026

Abstract （453）

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： Comprehensive analysis and summary of the current city-level forecasting business and meteorological service work characteristics and existing problems. In the process of forecasting product production, the problems of different sources of reference materials, various distribution channels, changeable business platform, and delay of early warning information transmission are discussed. A solution based on C/B/S meteorological disaster warning decision support system is proposed. First, it analyzes and classifies the auxiliary decision data source and the concrete forecast business production, and formulate mixed mode that use the multi thread C/S data processing and the B/S data display. In the overall framework of the system, the Oracle database storage service, the WebGIS technology, leaflet plugin, the Mybatis framework of the background service are used. The article elaborates on the technical design schemes of the system and gives the specific design method under the mixed mode. The actual operation results show that the model greatly improves the real-time and flexibility of the system, and the efficiency of forecasting business services has also been improved.

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Identification of Pu'er tea origin based on near infrared spectroscopy

LI Yu-jin, LI Chao, LI Jia-hua, SHI Wei, LI Zhe, YAO Ying-fang, ZHANG Yan-cheng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 138-141. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.027

Abstract （398）

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： 81 Pu'er tea samples from three different producing areas, such as Xishuangbanna, Pu'er City and Linyi City, were studied. Among them, 54 were corrected and 27 were verified. A single Pu'er tea sample was collected by near-infrared spectroscopy at 1100- The spectral data in the range of 2498 nm was established by principal component cluster analysis and discriminant analysis to establish a qualitative analysis model for Pu'er tea production. The test results show that the correct recognition rate of the correction set of the two analytical models is greater than 90%, which can be used for the identification of Pu'er tea origin. Among them, the discriminant analysis model based on the discriminant analysis method is better, and the correctness rate of the calibration set and the verification set is 98.15% and 100%, which is more suitable for the identification of Pu'er tea origin. Therefore, the application of near-infrared spectroscopy technology can quickly and non-destructively identify the origin of Pu'er tea, providing a new method for the detection of Pu'er tea production.

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Neighbor-based malicious node detection in wireless sensor networks

YANG Zhi-qiu, CHEN Li-min, ZHANG Dan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 142-144. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.028

Abstract （366）

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： Due to faults or malicious nodes,the sensor data collected or reported might be wrong. In this paper, we present a neighbor-based malicious node detection scheme for wireless sensor networks. Computer simulation shows that most of the malicious nodes reporting against their own readings are correctly detected unless they behave similar to the normal nodes.

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The interaction of Lj5NG4 and LjNFR1 in Lotus japonicus regulates the infection of Rhizobium

SONG Jia-bin, DUANMU De-qiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 145-151. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.029

Abstract （423）

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： Using the Lotus japonicus nodule factor receptor kinase LjNFR1 as a bait and a yeast membrane two hybrid library constructed with transcripts harvested at different stages of nodule symbiosis, we identified an auxin related transporter protein Lj5NG4. The interaction between Lj5NG4 and LjNFR1 was further confirmed by yeast two hybrid and Split-Luc experiments. Using CRISPR-Cas9 and overexpression technology, we conclude that Lj5NG4 may play a negative regulatory role in the early stage of rhizobial infection, but had no significant effect on nodule formation. Meanwhile, promoter expression also confirmed that Lj5NG4 was strongly expressed in root hairs at the early stage of infection, but not in inner mature nodules. Exogenously applied auxin treatment showed that low concentration of auxin weakened the phenotype of Lj5NG4 mutant, indicating that auxin plays a role in the development of symbiotic nodules.

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Moderating effect of brand reputation on network sales of agricultural products——Based on analysis of online comments

GUO Min, ZHOU Ya-xin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 152-156. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.030

Abstract （466）

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： Based on the survey data of 10 223 reviews on long-grain rice in Jingdong Mall, the effects of online custom reviews（OCRs） on product sales were analyzed. A factor model of long rice sales were set up to study the effects of brand reputation on product sales and verified by the actual crawler data. The results showed that the brand with high reputation had more significant regulating effect on the comments polarity, while the brand with low reputation had more significant regulating effect on the high-frequency words of product attributes. It provides a theoretical basis and method for online sales of agricultural products and online customer comment processing. It suggested that enterprises and e-commerce platforms need to develop differentiated strategies of product sales according to the brand and development process.

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Spatio-temporal coupling effect of rural poverty alleviation on county economic development：An empirical study based on 88 districts and counties in Guizhou province

KONG Shu-qi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 157-164. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.031

Abstract （373）

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： In this paper, the overall situation of economic development level and poverty incidence in 88 districts and counties of Guizhou province were analyzed by traditional statistical methods and exploratory spatial data. From the combination perspective of global and local space, the coupling interaction between county economic development and poverty were revealed to seek the coordinated development of regional economic development and poverty alleviation from the perspective of spatial coupling effect and provide a dynamic mechanism for the sustainability of precise poverty alleviation. The result showed that the regions with better economic development in Guizhou Province mainly distributed in the central, northern and northwestern regions. The overall distribution of poverty incidence is "U" type situation that low in the north-central, high in the southwest part of Guizhou Province. The poverty reduction rate in some parts of Northern Guizhou and southwest Guizhou presented more rapidly. The degree of coupling and coordination between economic development and poverty incidence showed a downward trend in the study period, and the spatial distribution was significantly different. The low coupling coordination area is mainly located in the districts of Guiyang City and the surrounding counties, which developed well in economic and fully played a radiation driving role and trickle-down effect of the economy. Highly coupled and coordinated areas are mainly located in southeastern Guizhou, which belong to spatial poverty trap area. It suggested that the policies such as relocation and industrial poverty alleviation, etc., should be continuously promoted in Guizhou Province to make up for the poverty-stricken districts and counties.

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Study on the innovation efficiency and influencing factors of agricultural science and technology in Xinjiang based on DEA-Tobi model

HE Feng-lin, WU Zheng-ping

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 165-168. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.032

Abstract （498）

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： Based on the data from 2007 to 2017, a model was established by using the DEA method to evaluate and analyze the comprehensive efficiency of agricultural science and technology innovation in Xinjiang, the integrated efficiency was decomposed into pure technical efficiency and scale efficiency, the utilization degree and expenditure scale of agricultural science and technology innovation in Xinjiang in each year were discussed. On this basis,a limited dependent variable random effect tobit model was built, the scale of Xinjiang agricultural science and technology innovation efficiency was as explained variables. The explanatory variable took in the actual observation of agricultural technology market turnover, agricultural R&D funds investment, agricultural technical personnel number, total power of agricultural mechanization, agricultural patent authorized number,fertilizer application, agricultural science and technology papers published and the total value of farm output. Tobit model was used to calculate the influencing factors and to analyze the influence degree.On the basis of the empirical results, according to the actual situation,some suggestions on the efficiency of agricultural science and technology innovation in Xinjiang were put forward.

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Study on the construction of auxiliary guarantee mechanism of public right of agricultural genetic resources based on benefit goal

LIU Lu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 169-172. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.033

Abstract （364）

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： With the development of biotechnology, people pay more attention to the external value of agricultural genetic resources, such as economy, bio-genetic and the internal value of social development and ecological construction,the contradiction between the protection and utilization of genetic resources is intensified. How to promote the protection of agricultural genetic resources in the process of utilization has become the focus of attention.On the basis of law, in the field of integrated environmental resources, economics and ecology, the legislative goal is to make sustainable use of agricultural genetic resources and to share the benefits of agricultural genetic resources equitably. With regard to the protection of agricultural genetic resources, there are two kinds of regulation by public law and private law. At the same time, it avoids the disorder competition problem caused by the regulation of single private law.In the use of agricultural genetic resources, through the realization of the right to know, common agreed terms, model contract provisions, establish a fair benefit-sharing mechanism to give full play to the role of agricultural genetic resources rights in promoting biodiversity.

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Challenges and countermeasures of exported agricultural enterprises against the background of the novel coronavirus pneumonia epidemic

LUO Peng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 173-175. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.034

Abstract （514）

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： Since the outbreak of the novel coronavirus pneumonia epidemic, China's export-oriented agricultural enterprises have encountered many challenges, such as production, transportation and international trade disputes. To address the plight of export-oriented agricultural enterprises, the Chinese government and local financial institutions have implemented a series of help measures. In combination with the current situation of the novel coronavirus pneumonia epidemic and legal rules,the challenges faced by Chinese export-oriented agricultural enterprises were combed,some suggestions to deal with them from the perspective of enterprises themselves and national governments were put forward, respectively, to provide a path choice for Chinese export-oriented agricultural enterprises to get out of the real predicament.

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Driving mechanism and evaluation of traditional village landscape under the background of rural revitalization strategy

HUANG Xue-hong, WU Rong-tao, SU Zhi-guo, YANG Fang-rong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 176-182. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.035

Abstract （397）

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： Taking 35 national traditional villages in the central Henan province as a sample, the landscape driving mechanism under the background of the rural revitalization strategy was stuedied from the perspective of the driving force of traditional village landscape changes. The results show that the lack of consideration of socio-economic factors is an important factor affecting and restricting the protection and development of traditional villages. The indicators of social and economic factors of the village are selected, and a comprehensive evaluation system model is constructed based on the harmony degree equation. The weight values of different socio-economic coefficients of traditional village selection are studied to promote the reasonable selection and effective protection of traditional villages and achieve the sustainable development of traditional villages as a whole.

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Thoughts on the development direction of Chinese agricultural products regional brand

ZHANG Mei, MA Zhong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 183-186. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.036

Abstract （407）

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： Developing agricultural products regional brand is an effective way to deepen supply-side structural reform in agriculture and it is also a key measure to solve a series of problems such as increasing agricultural production without increasing income, low quality balance, serious homogeneity of products and structural contradiction between supply and demand in the market. On the basis of literatures review about agricultural products regional brand,it is showed that more studies should be done in future studies in the following aspects,such as brand positioning,top-level design,quality assurance,traditional agricultural heritage protection and germplasm resource protection, so as to improve the theory system of agricultural products regional brand.

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Civil elites and grassroots governments：the dual forces in industrial transformation——A case study of Dan village,Jiangsu province

LI Hong-xiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 187-190. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.037

Abstract （357）

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： Based on Dan Village as an example, the role of private elites and grassroots governments in industrial transformation has been analyzed and discussed. The study found that the industrial transformation of Dan Village stems from the bottom-up autonomous exploration of folk elites and the follow-up advancement of the grassroots government from top to bottom. The private elite explored the industrial transformation alone before the grassroots government intervened. After its success, it used its own influence to drive the public to achieve transformation. The grassroots government is involved in the transformation in the context of the environmental problems caused by the original industry. It further promotes the industrial transformation of Dan Village by supporting early elites, creating a transformation atmosphere, improving infrastructure, providing technical training, and introducing preferential policies.

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Two-layer residents overweight influencing factors based on individual, community

TAO Xiao-fang,ZHU Zhan-qiang,ZHOU Su-hong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 191-196. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.038

Abstract （375）

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Based on 706 questionnaires collected from 26 typical communities in Guangzhou city，combined with community environmental survey， the residents' overweight prevalence and its influencing factors were investigated， as well as the community difference of overweight and its causes using multilevel linear regression model. The results showed that the overweight rate was 32.15%. In terms of individual factors， age was positively correlated with overweight probability. Married residents had a higher overweight probability than unmarried residents. The overweight probability of high-income residents was lower than that of lower-middle income residents. Walking fitness time was negatively correlated with overweight probability. In terms of the community environment， the number of park and squares around the community was negatively correlated with overweight probability. The residents’ overweight probability in indemnificatory housing and historic districts was higher than that of residents in commercial housing communities.Besides， the number of physical activity facilities around the community was positively correlated with overweight probability which defied common sense and the reason of this phenomenon was probably that overweight residents took the initiative to physical activities. There were community differences in residents’ overweight probability. Community physical activity environment could explain 2.03% of the community differences， while community physical activity environment and residential type could jointly explain 44.91% of the community differences. In a word，residents’ overweight probability was affected by both individual and community factors so it’s important to regulate excess weight at both levels.

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Endogenous mechanism principle promotes developing rural economy in new era

WANG Rui-juan, LUO Xin-yan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (5): 197-200. DOI: 10.14088/j.cnki.issn0439-8114.2020.05.039

Abstract （414）

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To realize the sustainable development of rural economy is the most important task for China to become a modern powerful country. In the new era, China's rural economic development is characterized by strong national policy supports, transforming economic development mode, renewal development concepts of farmer and rapid growth of rural economic development. However, at the same time of development, there are still deficiencies such as low power at implementation of rural economic development policy, fixed development model, passice development concept, and insufficient development motion, etc. Therefore, to realize truly sustainable development of rural economy and the transformation of rural economy from blood transfusion to hematopoiesis, the construction of policy endogenous mechanism must be strengthened, the concept of farmers' passive development must be changed, the mode of rural economic development must be activated, and the mechanism of talent introduction must be improved.

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Research and application of chitosan in aquatic animals

ZHANG Yi，ZHANG Zhi-hao，ZHANG Ji-ping

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 5-9. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.001

Abstract （667）

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Chitosa（COS） is a newly developed biologically active substance with immuno potentiating effect. It has various biological activities such as antibacterial， immune enhancement， hypolipidemic and anti-tumor， and has been widely used in food and medicine fields. The main biological functions of chitosan and their effects on aquatic animal growth performance， non-specific immunity and intestinal microorganisms were reviewed， and the mechanism of their effects was briefly explained， the prospect of its application was prospected，in order to provide a reference for the wide application of chitosan in aquaculture industry.

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Research advances on wheat powdery mildew resistance and molecular markers

ZHOU Jun, XU Ru-hong, XIE Xin, REN Ming-jian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 10-15. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.002

Abstract （668）

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Wheat powdery mildew is the main disease that harms the field of wheat. It has been proved that breeding and application of resistant varieties are the most economical, safe and effective measures to solve wheat powdery mildew. In recent years, the rapid development of molecular marker technology has laid the foundation for the research of resistant genes of wheat powdery mildew. The morphological characteristics, virulence frequency, source of resistance gene and chromosome location, physiological resistance and application of resistance gene of wheat powdery mildew were reviewed, in order to provide reference for wheat disease resistance breeding. Because the physiological races of pathogenic bacteria are extremely susceptible to mutation, which leads to overcoming the host's original resistance, different resistance genes can be effectively combined through gene aggregation, the resistance spectrum can be broadened, and the resistance can be lasted. And it is of great theoretical and practical significance to study the resistance of wheat powdery mildew.

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Research on social capital and performance of farmer professional cooperatives

LIU Jie, CHEN Xin-hua

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 16-19. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.003

Abstract （455）

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Combined with the research results of social capital theory of cooperatives at home and abroad in recent years, the existing research from three aspects were combed, which included the related research of social capital of cooperatives, the performance research of cooperatives and the relationship between social capital and the performance of farmer professional cooperatives, the cooperatives’ development trend were analyzed.

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Evaluation of environment and adaptability of varieties in the middle reaches of the Yangtze river for national winter rape regional experiment based on GGE double standard map

YU Ze-en, LUO Li-xia, ZHANG Fang, HONG Mei-yan, ZHANG Xiang-xiang, GUO Rui-xing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 20-27. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.004

Abstract （517）

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The high yield, stable yield and adaptability of the national winter rape variety regional test in the middle reaches of the Yangtze River from 2017 to 2018, and the test representative, discrimination and differentiation ability of each pilot were analyzed by using the GGE double plot method. The results that the main effects of genotype （G）, environment（E） and genotype by environment interaction （GE） were significant. Among them, E accounted for 48.25% of the treatment total variation in the sum of squares on average, whereas G and GE accounted for 15.15% and 36.60%, respectively. Liyouza178, Chuangzayou No6, Zhongyou 769, Huayouza 706, Zhongyou 619, Shengguang 168, Yuyou 39 and Jingyou 517 had excellent high yield, stability and adaptability. Hengyang city, Changde city, Yueyang city of Hunan province, Jingzhou city of Hubei province and other pilot sites have high comprehensive evaluation values, and have strong pilot representative, discrimination and differentiation ability.

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RS and GIS-based analysis on spatial-temporal evolution of soil erosion in Zhong county,Chongqing city

LIU Qian-nan, GUO Shan-xin, JIANG Xiao-li, WANG Jin, CHEN Jin-song

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 28-33. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.005

Abstract （537）

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Based on Landsat TM, Landsat TM8 OLI, RS, GIS and field remote-sensing survey data in 1990 and 2015, the spatial distribution of soil erosion pattern and its spatial-temporal evolution characteristics in Zhong county, Chongqing city were analyzed. The results shows that in these two periods, soil erosion was dominated by the micro erosion. The proportions of the soil erosion in 1990 and 2015 were 44.67% and 54.88%, respectively. The sumsof area proportions of the area in the mild, moderate and strong soil erosion type in 1990 and 2015 in this area is 50.99% and 41.59%, respectively. As the area of micro erosion increases, the area of other erosion types decreases. Strong erosion area showed the sharpest cut （25.77%） and mild erosion area narrows mostly（101.16 km²）. The great conversion happens between micro erosion and mild erosion, as the conversion between extreme strong erosions and strong erosions with other erosion types were relatively smaller.

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Exhibition design and popular science methods of Jiubujiang National Geopark Museum

CAO Li, YI Li-wen, XIE Bing-geng, LI Xiao-qing, ZHOU Xin-hua, YI Xi, YANG Hong-jue

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 34-38. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.006

Abstract （457）

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By making full use of the numerous geological relics and mature karst landscape in Jiubujiang National Geological Park, Jiubujiang National Geopark Museum built a new scientific palace about karst shows the Earth-geology landscape-karst Landscape-forest and wetland to visitors through many media methods, such as models, pictures, samples, movies, virtual experience and so on. The layout ideas and indoor popular science methods of Jiubujiang National Geopark Museum were summarized, and the popular science experience was popularized.

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Effects of biogas manure combined with chemical fertilizer on soil properties in cornfield

LIU De-yao, YOU Ying, XIA Jia-cheng, ZHAO Jin-xi, WANG Xue-fen, HAN Shou-liang, LIU Xin-cheng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 39-42. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.007

Abstract （407）

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In order to study the effects of combined application of biogas manure and chemical fertilizer in different proportions on soil properties, cornfield soil was selected as experimental material. Six different fertilization treatments were selected for corn field soil, including CK（no fertilization）, HF（chemical fertilizer）, ZH20（20% biogas fertilizer + 80% chemical fertilizer）, ZH50（50% biogas fertilizer + 50% chemical fertilizer）, ZH80（80% biogas fertilizer + 20% chemical fertilizer） and ZF100（100% biogas fertilizer）. Random block design was used. The effects of each treatment on soil bulk density, pH, organic matter content and available nitrogen, phosphorus and potassium content in five key maize growth stages, including jointing stage, small bell-mouthing stage, big bell-mouthing stage, tasseling and silking stage and physiological maturity, were studied by using random block design experiment. The results showed that the application of 100% biogas manure significantly decreased the content of soil organic matter at small bell-mouthing stage, but at physiological maturity it increased the content of soil organic matter significantly; The soil bulk density decreased in all fertilization treatments during the five growth stages, and the higher the concentration of biogas fertilizer, the more obvious the decrease of soil bulk density was; Compared with group CK, soil pH decreased in group HF, while increased in group ZH20, ZH50, ZH80 and ZF100 during the five growth stages; the application of ZH50 had the most significant effect on increasing soil available nitrogen content; The application of ZF100 and ZH80 had the most significant effect on increasing soil available phosphorus content; There was no significant difference among the effects on improving soil available potassium content of all fertilization treatments.

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Study on scale measurement of the stripping topsoil and medium-low-yield fields：Taking Changsha city for example

LI Zhuo-lin, LUO Shi-jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 43-50. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.008

Abstract （427）

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Under the circumstance that the cultivated land occupied by new construction land increases year by year, the method of stripping the soil occupied by construction of high-quality tillage layer is adopted to improve the middle and low yield fields, so as to improve the quality of middle and low yield fields and effectively protect cultivated land resources. With five districts and counties of Changsha city as the scope of research, based on the results of Changsha city’s land use status and overall planning data, cultivated land fertility data and others, the GIS spatial analysis and literature research are adopted to measure, evaluate and analyze the quantity and distribution of the medium-low-yield fields and the topsoil that can be stripped. The results showed that the theoretical volume of strippable topsoi is 15 118 600 m³, the volume of soil required to improve the low-yield fields is 37 413 100 m³, the supply of topsoil is far less than the demand. The potential of medium-low-yield fields is 60 195.11 hm², mainly distributed in Changsha county, Ningxiang city and Liuyang city; While the area of the strippable topsoil is 8 835.26 hm², mainly distributed in Yuelu district, so the supply and demand are unevenly distributed in space.

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Study on rainwater retention capacity of different greenland types in sponge campus

YANG Cai-qin, XU Yu-xia, CHEN Wei, MA Kai, HE Wen-xin, YANG Feng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 51-54. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.009

Abstract （441）

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In order to cope with the problem of water accumulation in every rain on campus, taking the eastern campus of Baoji University of Arts and Sciences as an example, the average soil moisture content of 0~30 cm under different vegetation conditions is measured by weighing method, and the distribution characteristics of different green space types are analyzed. SPSS 20.0 and other relevant software are used for statistical analysis of the measured data. The results show that the average soil moisture content shows surface layer（0~10 cm）>subsurface layer（10~20 cm）> 20~30 cm soil layer; The average soil moisture content of trees forest after rain Ginkgo biloba L.>Cedrus deodara （Roxb.） G. Don>Cerasus yedoensis>Sophora japonica>Diospyro skaki Thunb>Prunus cerasifera; The average soil moisture content of shrub grass after rain shows Photinia×fraseri Dress>stratified vegetation>lawn>Phyllostachys sulphurea>Euonymus japonicus Thunb. Sparse forest is easier to absorb precipitation than dense forest, tree forest contains higher water value than irrigated land, and grassland is easier to reduce precipitation loss than dense shrub forest.

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Study on time-varying relationship between greenhouse gas emission of animal husbandry and urban residents' income in Xinjiang

JING Pan, LI Li, YAN Jin-feng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 55-59. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.010

Abstract （375）

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Based on the analysis of the current situation of urban residents' disposable income and the greenhouse gas emission of animal husbandry in Xinjiang in recent 17 years, the relationship between urban economic development and the greenhouse gas emission of animal husbandry was discussed. The results showed that during 2001—2017, the greenhouse gas emission of animal husbandry decreased with the increase of disposable income of urban residents. The relationship between the disposable income of urban residents and greenhouse gas emission of animal husbandry can be divided into three stages. In the first and third stages, for every 1 000 yuan increase in per capita disposable income of urban residents, the increase in greenhouse gas carbon dioxide emission equivalent from animal husbandry was 147.8×10⁴ t and 16.1×10⁴ t, respectively, and the decrease in the second stage was 115.9×10⁴ t.

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Estimation of carbon emission from burning of agricultural crop straw in Hubei province

GONG Shi-fei, GUO Yuan-ping, YE Qing-song, LAN Yu-mei, WANG Wei, XIAO Neng-wu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 60-63. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.011

Abstract （419）

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In order to determine the carbon emission intensity from crop residue by open burning in field in Hubei province, based on date of crop yield from 2009 to 2017 in Hubei province, the major crop residues output were calculated according to the ratio of residue/grain. Combined with the proportion of straw open burning, the amount of straw open burning of main crops in Hubei province from 2009 to 2017 was calculated, and the total amount of CO and CO₂ emissions and carbon emissions were further obtained according to relevant emission factors. The results showed that annually production of crop straw in Hubei province was about 32.65×10⁶ t/a; The amount of openly burned straw in the field was about 7.01×10⁶ t/a, which accounted 21.47% of the total production of crop straw, among them, the open burning amount of rice straw accounts for 49.37% of the open buring amount of crop straw. The total amount of CO and CO₂ emitted by the open burning of crop straw was 0.72×10⁶ t/a and 9.74×10⁶ t/a, respectively; The total carbon emission was 2.96×10⁶ t/a on average, and the total carbon emission from the open burning of rice, wheat, corn and rape straw was 1.47×10⁶ t/a, 0.50×10⁶ t/a,0.52×10⁶ t/a,0.48×10⁶ t/a, respectively.

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Meteorological condition analysis of growth and development of rapeseed in Hubei province during 2018—2019

DENG Huan, QIN Peng-cheng, WAN Su-qin, DENG Ai-juan, JU Ying-qin, XIAO Wei-yu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 64-67. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.012

Abstract （434）

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The meteorological conditions of rapeseed growth and development in Hubei province from 2018 to 2019 were analyzed. The results showed that during the growth and development of rapeseed of 2018—2019 in Hubei province, the total heat quantity and rainfall in the main rapeseed-producing areas could meet its growing demand. However, there was a light deficiency. The seedling growth statues was weak because it went through agrometeorologica disasters like drought in the seedling stage, low air temperature and deficient light with rain and snow during the overwintering period. During the flowering stage the average lowest air temperature of the whole province was higher than the temperature criterion of chilling damage. There was no segmental fruiting phenomenon. The average air temperature of the whole province was lower than the suitable temperature for apothecia germination of Sclerotinia sclerotiorum. Moreover, there was no continuous rain and a mild occurrence of Sclerotinia sclerotiorum. During the pod-filling stage sunny day and rainy day alternated without a large scale of lodging. Compared with 2017—2018, there was similar meteorological conditions in the seedling stage. But the meteorological conditions showed different advantages or disadvantages during the overwintering period. There was better meteorological conditions this year than 2017—2018 from budding stage to pod-maturing stage in spring.

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Analysis on the spatial-temporal feature of PM2.5 concentration in Zigong city

WANG Ling-ling, HE Wei, ZHU Yu-lin, LUO Wei, HUANG De-gang, DUAN Xiu-rong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 68-72. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.013

Abstract （386）

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Based on the daily average air quality monitoring data of national control stations in Zigong area from 2014 to 2018, the temporal and spatial variations of PM2.5 concentration in Zigong city were analyzed by using multi-scale data. The results showed that the hourly variation trend of PM2.5 concentration in Zigong area was mainly related to human activities and solar irradiation time; The ratio of days of PM2.5 concentration per day reaching the first-class national air quality standard was 27.5%, and the ratio of days reaching the second-class standard was 73.5%; The period of high PM2.5 concentration was mainly in January, February and December; And it had the distribution characteristics of high in spring, autumn, winter and low in summer, and the proportion of days exceeding the standard in winter was as high as 69.02%. The spatial distribution characteristics of PM2.5 concentration were not only affected by the location of monitoring sites, topography, climate and urban structure, but also closely related to the contribution of wind direction and other pollution sources in surrounding cities. Overall, PM2.5 concentration levels were higher in Da'an, Gaoxin and Gongjing district with high industrial output, frequent transportation and dense population.

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The variation characteristics analysis of the initial frost day,final frost day and frost-free period in Luzhong area

DING Shan-wen, WANG Feng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 73-77. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.014

Abstract （549）

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In order to better grasp the impact of frost-free period on agricultural production in Luzhong area and improve the prediction of frost, making full use of national meteorological stations in Luzhong area to use linear trend estimation method, Mann-Kendall mutation analysis, T Sliding, ArcGIS spatial interpolation and other methods analyze the temporal and spatial variation characteristics of frost, the first day and the frost-free period and their relationship with climatic factors and geographical factors in Luzhong area. The results showed that the first frost day was significantly delayed, the final frost day was significantly advanced and the frost-free period was significantly extended in the Luzhong area. The years of mutation were 2003 and 1996 and 2003, respectively. The initial frost day and final frost day and frost-free period were closely related to temperature. The distribution of initial frost day and final frost day and frost-free period was uneven. The first frost days appeared earlier in the central mountainous areas, and gradually delayed from the east and west to the sides. The final frost day first appeared in the northwest. Later was the central and Changyi-Weifang-Anqiu area and the latest was Laiwu, Linqu, Weifang area. The frost-free period was the longest in the northwest, and the shortest appeared in the central and southern mountains. The initial frost day and the final frost day were positively correlated with altitude and longitude and respectively reached a very significant level. The frost-free period was negatively correlated with altitude, and reached a significant level with the influence of altitude.

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The analysis of the character and causes of the disaster of the “16·7” rainstorm in Hebei province

HU Hui-fang, JING Hua, WEI Jun, ZHANG Su-yun, LI Jiao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 78-83. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.015

Abstract （800）

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The character and causes of the disaster of the “16·7” rainstorm in Hebei province were analyzed. The “16·7” torrential rainfall was the second since the meteorological record in Hebei province. The rainstorm coverage set a new record, daily precipitation exceeded the extreme value of July, precipitation concentrated in the Taihang mountains, and the rain was strong and lasted for a long time. It caused 10.436 million people was to be affected, and the direct economic loss was 57.457 billion in Hebei province. From the perspective of the affected industries, infrastructure losses was the most serious; From the disaster-stricken areas, the Taihang mountains in the south-central region were the heaviest, especially in Shijiazhuang, Xingtai and Handan. The main reasons of the disaster wereThe precipitation intensity was large and lasted for a long time, and some areas in the mountainous areas reached once in a hundred years; The Taihang mountain had a large elevation difference, and topographical factors lead to flooding, which was the secondary factor for disasters; A variety of severe weather concurrency, heavy rains in the Taihang mountain were accompanied by strong winds, and coastal winds caused storm surges, aggravating disasters; Unscientific human activities occupying the flood channel were also one of the reasons for the expansion of the disaster.

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Isolation and identification of pathogen of pea fusarium wilt in Chongqing city

ZHANG Hui, MA Lian-jie, FENG Mu-ye, LU Wen-cai, LIAO Dun-xiu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 84-87. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.016

Abstract （356）

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The tissue isolation method was used to isolate and purify the pathogen of spot of fusarium wilt of peas in Chongqing city, and the isolates were identified by combining with the analysis of morphology and gene sequence. The pathogenicity of the isolates to the leaves and stems of isolated peas was determined by the method of inoculation without injury. The results showed that five strains were isolated from infected leaves. The colony was round, white flocculent in the early stage and yellowish brown to brown in the later stage; The homology of ITS sequence and G3PDH gene sequences of the tested strain reached 99% with that of Ascochyta pinodes; The results of pathogenicity determination showed that the strain had pathogenicity to both leaves and stems of pea. It indicated that Ascochyta pinodes was the cause of pea blight in Chongqing city.

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Field efficacy test of 3% diflufenican granules to prevent broadleaf weeds in rice transplanting field

LI Jun-jun, DAI Ling-ling, BAI Xin-sheng, GU An-le, BI Ya-ling

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 88-90. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.017

Abstract （934）

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In order to identify the effective agent to prevent and remove broadleaf weeds in rice transplanting field, the control effect and safety of 3% diflufenican granules on broadleaf weeds in rice transplanting field were studied. One day before rice transplanting, the field was evenly distributed with the herbicide, and the growth of rice and the control effect of broadleaf weeds were investigated regularly after spraying herbicide 20, 40 days. The results showed that 3% diflufenican granules could effectively prevent and eliminate annual broadleaf weeds such as Ludwigia prostrata, Monochoria vaginalis, Eclipta prostrata. After application, the inhibition effect of 3% diflufenican granules on the growth of weeds was obvious, and it was safe for rice. It was recommended to apply 3% diflufenican granules with the dosage of 135~225 g（a.i）/hm².

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Effect of mesosulfuron-methyl·florasulam·fluroxypyr-meptyl on weed control,nutrient and yield in wheat field

ZHU Wen-da, YAN Dong-dong, LI Lin, CAO Ao-cheng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 91-95. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.018

Abstract （562）

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The effect of 26% mesosulfuron-methyl·florasulam· fluroxypyr-meptyl oil dispersion（OD） on weed control in wheat field was studied. The wheat yield, the content of N-P-K and water were also analyzed after weed control. The results indicated that the efficacy of weed control was remarkable by 26% mesosulfuron-methyl·florasulam· fluroxypyr-meptyl OD in wheat field. The control efficacy of compound formulation on Malachium aquaticum, Galium aparine, Vicia sativa and Beckmannia syzigachne was ranged from 91.5% to 97.3%, which was significantly higher than three individual herbicides. The accumulation of weed water and nutrients was significantly decreased after weed control. The wheat yield was 3 456.27 to 3 591.27 kg/hm² after treated with 26% mesosulfuron-methyl·florasulam· fluroxypyr-meptyl OD, which increased by 36.0%~41.2% compared to untreated control. Moreover, the yield income increased by 2 101.65~2 412.15 Yuan/hm².

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Effect of florasulam·halosulfuron-methyl on weed control,nutrient and yield in wheat field

ZHU Wen-da, YAN Dong-dong, LI Lin, CAO Ao-cheng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 96-99. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.019

Abstract （674）

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The effect of 75% florasulam·halosulfuron-methyl WDG on weed control in wheat field was studied. The wheat yield, the content of N-P-K and water were also analyzed after weed control by florasulam·halosulfuron-methyl. The results indicated that the efficacy of weed control were remarkable by 75% florasulam·halosulfuron-methyl WDG in wheat field. The control efficacy on Galium aparine,Malachium aquaticum and Vicia sativa was 92.9%~99.7%. The accumulation of weed water and nutrients was significantly decreased after weed control. The wheat yield was ranged 3 059.33 to 3 248.40 kg/hm² after treated with florasulam·halosulfuron-methyl, which increased by 25.70%~33.47% compared to untreated control. Moreover, the yield income increased by 1 438.83~1 873.70 Yuan/hm².

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Effect of dazomet soil disinfestation on weed control,nutrient and yield in wheat field

ZHU Wen-da, YAN Dong-dong, LI Lin, CAO Ao-cheng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 100-103. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.020

Abstract （433）

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The effect of 98% dazomet GR on weed control in wheat field was studied. The wheat yield, the content of N-P-K and water were also analyzed after weed control by dazomet. The results indicated that the efficacy of weed control was remarkable by 98% dazomet GR in wheat field. The control efficacy of dazomet on Beckmannia syzigachne, Galium aparine, Malachium aquaticum and Vicia sativa was ranged from 87.5% to 99.5%, which was significantly higher than the two control herbicides. After weed control, the consumption of nitrogen, phosphorus, potassium and water in the field was significantly reduced, and the water and nutrients conditions in the field were effectively improved. The wheat yield was ranged from 2 970.01 to 3 123.77 kg/hm² after treated with dazomet, which increased by 28.9%~35.6% compared to untreated control. Moreover, the yield income increased by 1 532.38~1 886.02 Yuan/hm².

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Research on traditional village narrative space based on space syntax：Taking Zhangdian village,Jia county,Henan province as an example

XU Bo-lun, LI Jiao, FAN Xue-qin, YANG Fang-rong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 104-107. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.021

Abstract （526）

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Traditional villages carry people's memory and homesickness, as well as hundreds of thousands of historical stories. Studying the narrative of village space is for better memory. Taking Zhangdian village, Jia county, Henan province as an example,the main narrative elements of its place space was explored, the path of its events and space was analyzed, the space syntax theory and depth map software Depthmap were used to quantitative analysis the narrative space, and some corresponding suggestions for the protection of the traditional villages were puts forward.

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Study on cold resistance of Chaenomeles ornamental plants based on Logistic equation analysis

TANG Wen, CHEN Jian-miao, ZUO Jin-fu, WANG Yang, WANG Chao-yang, HUANG Fang-wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 108-111. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.022

Abstract （359）

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In order to study the cold tolerance of ornamental plants of the genus Chaenomeles, three different types of Chaenomeles trees were selected as research objects, and their water content, starch content, soluble sugar, and peroxidase（POD）, superoxide dismutase（SOD） and relative conductivity and other physiological and biochemical indicators from September to January of next year. Then the Logistic equation was used to solve the semi-lethal temperature, and the membership mean was calculated by the subordination function method to comprehensively evaluate its cold resistance. The results showed that with the increase of cold resistance, the soluble sugar content, POD activity and SOD activity all increased, and the change trend was the same as that of cold resistance. The cold resistance of the stems of the three tree species was Chaenomeles japonica （Thunb.） Lindl. ex Spach<Chaenomeles speciosa （Sweet） Nakai<Chaenomeles sinensis （Thouin） Koehne.

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Quantitative analysis of visual attraction of urban waterfront greenway landscape：Taking the waterfront greenway of Dongfeng canal in Zhengzhou as an example

HE Xue-hui, YANG Fang-rong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 112-116. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.023

Abstract （523）

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Aiming at the problems of such as the destruction of visual resources, model development and lack of characteristic features of waterfront greenway landscape, from the perspective of visual attraction, taking the Zhengzhou Dongfeng canal waterfront greenway as an example, the basic data of 10 indicators such as spatial scale, plant, entity, color, texture, water, dynamic scene, landscape diversity, landscape naturalness and landscape coordination were obtained by OpenCV and SBE methods respectively, then combined with the entropy method to calculate the weight,the factor correlation and stepwise regression analysis were made by SPSS 25.0 software. Finally, the visual attraction model was established and the greenway was evaluated. The results showed that the visual attraction quality of Dongfeng Canal's waterfront greenway was high, Wenbo East Road-Garden Road Section and North Third Ring Road-Xuzhai Road Section were the highest, and Dongming Road-Zhongzhou Avenue section was the lowest, and the difference was significant.

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Evaluation and analysis of waterfront plant landscape in urban specialized parks：Taking Zhengzhou sculpture park as an example

YU Bing, YANG Yan-ji, LIN Cheng-wang, FENG Li-ming, WANG Peng-fei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 117-125. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.024

Abstract （466）

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Taking Zhengzhou sculpture park as the research object, 16 waterfront plant landscape units are selected and integrated with AHP method, landscape aesthetic index method, BIB-LCJ method and SD method to establish a comprehensive evaluation model. The results show that although there are differences between professionals and the public in the evaluation of individual factors, the overall consistency is maintained. The overall plant landscape aesthetics index of the sculpture park is 6.72, which belongs to the general to better level and has research value and promotion space. Factor analysis of four waterfront plant landscape units with scenic beauty greater than 1.500 is carried out by SPSS 25.0 software. The four elements that affect the landscape quality are characteristic factor, cognitive factor, perception factor and experience factor. The contribution rate of characteristic factors is the largest. To improve the quality of waterfront plant landscape, we should not only ensure the basic species richness and water ecology, but also pay attention to the use of ornamental characteristics such as plant color, enhance local landscape characteristics, and shape the theme of special parks with vitality plant elements.

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Explorationon development route of beef cattle industry in Hinggan League

GAO Xiao-xia, YAN Qing-qi, ZHAO Jun-li, HOU Zhi-hui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 126-129. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.025

Abstract （432）

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In order to promote the sustainable and high-efficient development of beef cattle industry in Hinggan League, the development status and features of beef cattle industry in this region were summarized in this article from the following aspects of beef cattle production, regional layout, slaughtering processing and sales, technical services and industrial poverty alleviation. Furthermore, the advantages and constraints in development of beef cattle industry in Hinggan League were analyzed, and the corresponding countermeasures and suggestions were proposed.

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Prokaryotic expression of mouse extracellular domain of CD40 and preparation of polyclonal antibody

CAO Qi, PAN Yue, ZHENG Yu, WANG Han, REN Hong-lin, HU Pan, LI Yan-song, ZHOU Yu, LIU Zeng-shan, LU Shi-ying

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 130-134. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.026

Abstract （430）

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To investigate the enhanced effect of CD40 antibody binding to CD40 on B-cell response to small molecule haptens,in this study, the total RNA of BALB/c mouse spleen was extracted by TRIzol method and reverse transcribed into cDNA; Primers were designed based on the CDS region of the extracellular domain of CD40, and the target gene was amplified by PCR to construct the pGEX4T-1-CD40 expression vector for prokaryotic expression;The polyclonal antibody was prepared. The results showed that the 522 bp target gene was successfully amplified, CD40 recombinant protein of 45 ku was expressed and purified. The antibody obtained by Western-blot detection not only binds to recombinant protein, but also can specifically bind to mouse spleen natural CD40 protein. Those indicated that the expression of CD40 recombinant protein is successful, and the prepared antibody was expected to mimic CD40L to provide the second signal for B cell activation.

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Effects of chitooligosaccharide on the muscle nutritional components of Yellow river carp

LYU Cheng-cai, FAN Chao-jie, XIONG Jian-li, CHEN Wan-guang, CHAI Jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 135-138. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.027

Abstract （417）

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To study the effects of chitooligosaccharide on the muscle nutritional components of Yellow river carp, a total of 270 Yellow River carp with initial body weight of （34±3） g were randomly assigned to six treatments which feed different concentrations （0%, 0.2%, 0.4%, 0.6%, 0.8%, 1.0%） of chitooligosaccharide for 8 weeks, each treatment had 3 replicates. The results showed that moderate concentration of chitooligosaccharide can improve the protein and ash contents, reduce the water content; change the components of amino acid content, improve the muscle quality, but not affect the muscle relish. The most optimum additive amount in Yellow river carp was 0.6%.

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Comparision of nutrient quality of wild and cultural Scylla paramammosain

HUANG Ai-xia, LIN Feng, SUN Li-hui, JIANG Rong-xiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 139-142. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.028

Abstract （409）

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The muscle and sexual gonad were separated from wild and cultural Scylla paramammosain （six of each kind, half male and female） for nutrient analysis. The results showed that the differences in moisture, crude protein, crude fat and crude ash between the muscle and gonad of wild and cultured Scylla paramammosain were significant （P<0.05）. There were differences between the 17 amino acids detected in muscles and gonads of wild cand cultured crabs. The total amount of flavor amino acids （FAA） in the muscles of wild crabs was significantly lower than that of cultured （P<0.05）, and the proportion of essential amino acids （EAA） was significantly higher than that of cultured （P<0.05）. There were also differences in the composition of fatty acids in the muscle and gonad of wild and cultured crabs. The fatty acid composition of the two crabs has their own advantages.

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Screening and identification of protease-producing bacteria from alkaline fermentation of excess sludge

JIE Wei-guang, YAO Yan-xuan, PENG Yong-zhen, HU Wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 143-147. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.029

Abstract （421）

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In order to shorten the alkaline fermentation period of excess sludge, alkaline-tolerant strains with high protease-producing activity were isolated from alkaline fermentation of excess sludge and identified by morphological. physiological, biochemical, and molecular characteristic analysis. The results showed that the two screened alkali-tolerant bacterial strains （HIT-01 and HIT-02 strains） which of high protease-producing activity were belongs to Bacillus subtilis and Bacillus sp. This study opens a new idea for further utilizing the screened dominant strains to construct microbial agents and quickly activate alkaline fermentation of sludge.

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Preparation of basil essential oils and its application in making cigarette

LI Cheng-bin, ZHANG Jun-song, YANG Shang-ying, LIU Yu-yu, YUAN Lin-cui, YANG Yan-mei, BAO Xiu-ping

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 148-152. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.030

Abstract （386）

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In order to improve the utilization efficiency of basil essential oils extracted by solvent-free microwave extraction （SFME） and steam distillation （SD）, the effect of the two methods on the quality of basil oil, such as yields, aroma quality, volatile components, and its application in cigarette, etc., were evaluated. The results showed that the yield of basil oil by SFME was significantly higher than that by SD. The main aroma components in the basil essential oils by both methods were estragole, but the content in basil essential oil extracted by SFME was 10% higher than that by SD. And other components in the basil oil by the two methods are also with a little different in variety and contents. The application in cigarette showed that the basil essential oil by SFME could improve the sense of sweet and enrich the aroma of smoking.

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Analysis of volatile components of different processed products of Phytolacca acinosa Roxb. by HS-SPME-GC-MS

MA Yin-yu, LU Jin-qing, DENG Ya-qian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 153-156. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.031

Abstract （467）

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The volatile components of different processed products of Phytolacca acinosa Roxb. were compared and analyzed by headspace solid phase microextraction （HS-SPME） and gas chromatography-mass spectrometry （GC-MS）. The relative percentage of each component was calculated by area normalization method. Results showed that 39 peaks were detected and 29 constituents were identified, accounting for 76.52% of the volatile constituents. 33 peaks were detected and 27 constituents were identified from vinegar Phytolacca acinosa Roxb., accounting for 68.78% of the volatile constituents. There were 14 volatile components in both. Compared with Phytolacca acinosa Roxb. vinegar Phytolacca acinosa Roxb. has 15 components reduced and 13 components added.

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Determination and law of selenium content in soil and crops of Hanwang town Ziyang county

LIAO Biao, JIN Hua-feng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 157-159. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.032

Abstract （527）

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Hanwang town of Ziyang county was taken as the research object, and the selenium content of some cultivated soils and growing crops in the area were analyzed. The results showed that 619 soil samples were naturally containing selenium, and the highest selenium content reached 44.504 6 mg/kg, with an average of 1.694 3 mg/kg. Among them, 342 selenium-rich soil samples accounted for 55.25% of the total samples; A total of 332 samples were collected, with an average selenium content of 0.284 1 mg/kg, which reached 115 standard samples of selenium enrichment in Shaanxi province, accounting for 34.64% of the total. The average selenium content of the soil was high, and the average selenium content of the growing crops is correspondingly increased. The selenium content of the crops has a certain correlation with the selenium content of the soil.

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Improved approach for multi-attribution group decision making based on interval fuzzy evidential reasoning and TOPSIS method

CAO Cheng, GUO Min

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 160-164. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.033

Abstract （372）

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Combining interval fuzzy evidential reasoning（FER） and technique for order preference by similarity to ideal solution（TOPSIS） method, and TOPSIS group decision-making method was proposed. ER’s distributed expression framework can solve more complex uncertain multi-attribute group decision-making problems, meanwhile, expert reliability was used to modify the expert weight given by subjective experience, which can improve the rationality of ER algorithm applied in MAGDM problem.

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Thinking and countermeasures of improving and strengthening kiwifruit industry in Yichang city

LU Meng-ling, WANG You-hai, LYU Zhi-zao, YAN Hua-jie, CHEN Dan-dan, LI Hong-chang, LYU Min, HUANG Sheng-dong, LI Qiong-jie

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 165-168. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.034

Abstract （426）

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Based on the investigation and analysis of the current situation and existing problems of kiwifruit industry in Yichang city, the following suggestions were put forward, which included strengthening organizational leadership, integrating scientific and technological resources, scientifically planning layout, optimizing variety structure, improving infrastructure, extending industrial chain, increasing investment in scientific research, highlighting scientific and technological support, focusing on personnel training, strengthening team construction, increasing warning publicity, raising awareness of prevention and control, expanding brand influence, enhancing brand value.The suggestions can provide reference for promoting the healthy and sustainable development of kiwifruit industry in Yichang city.

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The influencing factors and countermeasures of the choice of “House-For Pension”：Taking Hangzhou city as an example

ZHOU Ping

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 169-172. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.035

Abstract （405）

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“House-For Pension” is a new way of pension. Taking the elderly residents of Hangzhou city as the research object, the factors influencing of the choice of “House-For Pension” model were analyzed through questionnaires and field surveys. By using logistic regression model, we found that 11 variables had significant influence on the choice of“House-For Pension”,which included the sex of Elderly Residents in Hangzhou city, the monthly income level, the education level, the physical condition, the relationship with children,the satisfaction with existing pension resources,the attitude towards“Supported by the Son”, the understanding of “House-For Pension” policy,the spiritual consolation and economic support of children,the times of the elderly taking care of their grandchildren. Based on the research results, the relevant countermeasures were proposed.

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Study on the legalization of rural governance under the background of rural revitalization

MA Zhan-ying

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 173-177. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.036

Abstract （549）

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By explaining the importance and necessity of the rule of law in promoting rural rejuvenation, some rural issues was Inductively analyzed, such as inadequate rural laws and regulations, weak peasants' awareness of the rule of law, and inadequate legal capacity of grass-roots cadres. Specific countermeasures have been proposed, such as improving the legal and regulatory system, strengthening the popularization of laws, and improving the legal quality of grass-roots cadres, so as to better promote the construction of legal countrysiole and promote rural revitalization.

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Research on measurement and improvement path of integration degree between advanced manufacturing industry and modern service industry ——Taking Shanxi province as an example

SHEN Dan-hong, LI Xiao-ming

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 178-183. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.037

Abstract （490）

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Based on the background of the transformation in the development of Shanxi province, around the fusion advanced manufacturing and modern service industry development theory, by analyzing the province both internal structure change trends and industry foundation, the measurement fusion of subdivision industry development degree, advanced manufacturing and modern service industry in Shanxi province fusion development trend and the foundation, on the whole the fusion development degree is higher. Finally, according to the existing integration foundation, the advantages and disadvantages of the industry and the supply and demand relationship in Shanxi province, the characteristic integration path was constructed, and the countermeasures and suggestions were put forward from the angle of industry division.

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Thoughts on weaknesses of China’s agricultural technology

QI Xiao-zhe, LUO Yun-bo

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 184-188. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.038

Abstract （505）

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Aiming at the weaknesses of agricultural technology development in China, the methods of classification analysis were applied to analyze each one. The results showed that, the weaknesses of agricultural technology development in China are mainly the following aspects, including grain security, storage and transportation of agricultural products, pesticide production technology, animal breeding technology, food nutrition and health, diet and chronic diseases. In order to solve the above shortcomings, it is necessary to reduce the loss of grain circulation, improve the fresh-keeping capacity of agricultural product logistics, increase the competitiveness of pesticides, increase investment in animal breeding, develop functional agricultural industries, and strengthen research on diet nutrition and chronic diseases. At the same time, we must deepen agricultural supply-side structural reforms, allow scientific and technological innovation to lead the development of agricultural technology, and realize the agricultural development vision of "weaknesses, weaknesses, and strengths".

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Research on the perfection of the system of restrictive norms for the transfer of agricultural water rights

WANG Cong-cong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 189-192. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.039

Abstract （370）

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Based on legislative considerations such as the protection function and transfer risk of agricultural water rights, the current law has formulated a number of restrictive regulations on the transfer of agricultural water rights, such as "the subject of transfer is limited to internal members". From the perspective of private law, agricultural water rights have the nature of usufructuary rights and enjoy full legal powers of punishment. According to the perspective of power analysis, the existence of existing restrictive norms has reflected a lot of irrationality. To this end, we can try to take comprehensive measures such as strengthening the economic function of agricultural water rights, abandoning relevant restrictive norms in the current law, and strengthening the government's management and services of circulation, so as to achieve the scientific allocation of restrictive norms of agricultural water rights circulation.

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Research on new media construction of rural river chief system from the perspective of environmental communication

ZHANG Jie, WANG Rong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 193-197. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.040

Abstract （369）

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The cause of the imbalance in the new media coverage of the current rural river chief system issue is clarified that the reporter is affected by the effect of the third person, and the current rural residents' new media usage habits are analyzed. Faced with reports on environmental issues, new media should do a better job of constructing rural issues and grasp the discourse framework so that the public can effectively participate in rural governance issues such as the river chief system, and promote the overall progress of rural water environmental governance and the comprehensive development of farmers.

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Inter-provincial scientific and technological innovation efficiency evaluation and analysis of influencing factors along the “Belt and Road”

CHEN Cong-cong, MA Xiang-ping

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 198-202. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.041

Abstract （407）

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By selecting statistical data from 2012 to 2017, a data envelopment analysis （DEA） method was used to construct a two-stage scientific and technological innovation efficiency evaluation index system. Based on the input-output perspective, the technological innovation efficiency of the provinces along the “Belt and Road” was measured, and projection analysis was used to explore the influencing factors of their spatial and temporal differences. Some suggestions were provided for improving the efficiency of scientific and technological innovation in various provinces and rationally allocating scientific and technological innovation resources..

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Evaluation of county-level agricultural leading industry based on AHP-fuzzy comprehensive evaluation model——Based on County A in Shandong province

PAN Fei, ZHANG Zai-xu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (6): 203-209. DOI: 10.14088/j.cnki.issn0439-8114.2020.06.042

Abstract （353）

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Combined with the development characteristics of County A in Shandong province, the evaluation principles and evaluation indicators for the county's leading agricultural industries were established, and the AHP-fuzzy comprehensive evaluation model was used to quantitatively evaluate County A and the leading agricultural industries. Vegetables and aquatic products were the leading agricultural industries in the county. The evaluation conclusions, put forward countermeasures and suggestions to improved the development of vegetables and other industries.

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Metabolic inheritance and ecological adaptation mechanism of plants responding to selenium

ZENG Wei-chao, CHEN Da-qing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 5-10. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.001

Abstract （475）

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The absorption and tolerance of selenium by plants are the key factors in the production of selenium rich foods, the research progress of selenium rich metabolism, molecular mechanism and ecological function of plants at home and abroad was reviewed from the perspective of biochemistry, physiological genetics and ecology, and the research trend of plants and selenium was prospected.

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Application of isotopic tracer technique in soil selenium transformation and research progress of plant availability of selenium

YU Feng-yuan, JIANG Dai-hua, LI Sheng-hui, WANG Shi-jia

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 11-15. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.002

Abstract （413）

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Selenium is an essential trace element for human beings and animals. The application of isotopic tracer technology in the transformation of selenium and its soil and the availability of plants are reviewed, and the development trend of isotopic tracer technology of selenium is prospected.

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Research progress and prospect of lake wetland vegetation succession

HU Dan-ying, ZHAO Fei, YUAN Long-yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 16-19. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.003

Abstract （438）

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As a part of terrestrial water circle, lake wetland is involved in the water cycle in nature, and the dynamic succession of vegetation directly reflects the health status of lake ecosystem. The research status of influencing factors of vegetation succession is reviewed, the research methods used in the process of exploring vegetation succession are introduced, and some cases in practical application are shown, and the future research on vegetation succession is prospected.

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Active components of rice and its mechanism of action

LI Xiao-xiao, LI Zhi-bin, WANG Fang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 20-23. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.004

Abstract （453）

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The active components found in rice over the years are summarized,and these active ingredients are mainly rice polyphenols, γ-aminobutyric acid（GABA）, resistant starch（RS）, oryzanol, sterol and octacosanol,their mechanism of action. Furthermore, the mechanism of action is described systematically, and the clinical trials of the active components confirmed by the results are summarized.

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Comparative study on different seeding planting methods of cotton in hardened groud in cotton-planting area of northern Jiangxi province

LIU Xin-wen, WANG Tao, YANG Shao-qun, DENG Yan-feng, YANG Xiu, KE Xing-sheng, XIAO Shui-ping

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 24-27. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.005

Abstract （423）

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Three kinds of direct seeding planting methods commonly used by cotton farmers（code A： trench strip sowing, code B： bamboo tube on-demand, code E： trench on demand） and two new kinds of cotton board direct seeding planting methods （code C： burrowing sowing with covering fertilizer, code D： burrowing sowing with covering soil） in northern Jiangxi province were used planting cotton. The using time, labor intensity, amount of seeds weight, emergence rate and agronomic traits of cotton under different direct seeding mode was compared to find a suitable way for direct seeding planting to reduce the labor intensity of cotton farmers, reduce the labor input and increase the benefit of cotton planting in northern Jiangxi. The results showed that five different direct seeding planting methods had no significant effect on the agronomic characters and fiber quality of cotton, and the emergence rate of each direct seeding method was better, above 85% （except A）. The performance of mode A was less saving time and not saving effort, which was greatly affected by cotton field moisture. The performance of mode B was saving time and effort, but it had strict requirements for cotton field moisture, which was used according to soil moisture. The comprehensive performance of mode C was better, which was saving time and less saving effort. The performance of mode D was less saving time and not saving effort, which had strict requirements for cotton field moisture. The performance of mode E was saving time and not saving effort, which was greatly affected by cotton field moisture. Comprehensive analysis, burrowing sowing with covering fertilizer （code C） and bamboo tube on-demand （code B） had good comprehensive performance. They achieve the purpose of light simplification of planting, which are suitable for large area planting and further demonstrated and popularized in cotton-planting area of northern Jiangxi.

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Effects of different potassium fertilization modes on yield, quality of lettuce and soil regime

LI Chun-ping, WANG Su-ping, DU Lei, HONG Juan, HUANG Xiang, ZHANG Gui-you, ZHANG Li-hong, YE Li-xia, LIAN Zhi-cheng, JIANG Li, CHEN Gang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 28-32. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.006

Abstract （461）

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In order to obtain high quality organic potassium fertilizer suitable for lettuce production, a potted lettuce experiment was set to study the effect of different organic potassium fertilizer application on lettuce （Lactucas sativa L. var. ramosa Hort.） yield, quality and soil potassiums. The results showed that the application of potassium fertilizer could significantly increase the yield and quality of lettuce, and significantly improve the soil enzyme activities, microbe quality and nutrient status. Under the condition of equal application of potassium, 1∶1 application of chemical potassium fertilizer and biogas slurry can significantly improve the yield of lettuce. Its yield increased by 6.34%~30.53% compared with other potassium fertilizer treatments. The content of VC and soluble sugar of the treatment 1∶1 application ratio of chemical potassium fertilizer and biological bacteria fertilize postassium was the highest,followed by T₄. The combination of chemical potassium fertilizer and corn or rice straw potassium can significantly improve the soil conditions, the soil enzyme activity and microbial condition were significantly better than other treatments, the combination of chemical potassium fertilizer and biogas slurry were the second. Therefore, we can choose biogas slurry or straw as the source of organic potassium fertilizer in lettuce planting.

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Effects of different nitrogen fertilizer regulation on tiller number and leaf area index of rice

ZHOU Li-yan, HUANG Ying-hua, ZHANG Shan-xuan, GUO Zhi-yao, HONG Rui-xia, WANG Yu-lin, XIA Li-sha, FU Lu, LI Yan-da, CHEN Qing-chun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 33-37. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.007

Abstract （437）

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In order to clarify the effect of nitrogen fertilizer application on the number of tillers and leaf area index of rice, the main varieties of Yongnongsimiao and Hemeizhan were used as test materials, and five nitrogen fertilizer levels were set for the experiment. The results showed that during the same growth period, the total stem tiller number, ineffective tillers and leaf area index of rice plants increased with the increase of nitrogen application rate; among them, the nitrogen application amount, tiller number and leaf area index, there was a significant positive correlation between them （r>0.8）. Appropriate amount of nitrogen application can increase the number of tillers and leaf area index of rice and promote its growth and development, but excessive application of nitrogen fertilizer will increase the number of ineffective tillers and reduce the utilization rate of nitrogen fertilizer.

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Effect of a formulated foliar micro-fertilizer Lingdan on fruit production of Juglans regia

CHEN Wei, ZHUO Ren-fa, XIA Ling-yu, LI Shuang-long, DUN Chun-yao, CHEN Bei-bei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 38-40. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.008

Abstract （574）

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A 6-year-old forest age was selected in the Juglans regia fruit forest as material. The effects of the formula type foliar micro-fertilizer Lingdan on yield per plant and some traits affecting nut quality were studied. The results showed that the application of formula type foliar micro-fertilizer Lingdan had a great influence on the yield and agronomic traits of Juglans regia fruit. Applying the formula type foliar micro-fertilizer Lilydan can increase the amount of dried fruit produced, increase the kernel rate, and reduce the shape of Juglans regia fruit and the weight of Juglans regia shells, but does not affect the thickness of the shell.

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Effects of different base fertilizers, and ratios of nitrogen, phosphorus and potassium topdressing on the growth of Camellia nitidissima seedlings

CHAI Sheng-feng, TANG Jian-min, ZOU Rong, WEI Ji-qing, TANG Hui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 41-45. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.009

Abstract （501）

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In order to improve the growth rate of Camellia nitidissima and realize its early flowering and premature birth, the effects of different base fertilizers, and ratios of nitrogen （N）, phosphorus （P） and potassium （K） topdressing were studied. The results showed that chicken manure and garbage sludge as base fertilizers had obvious promoting effect on the growth of Camellia nitidissima, but pig manure had poor effect. The medium level of N and P and low level of K topdressing had the best effect on the growth of Camellia nitidissima. It was beneficial to the growth of Camellia nitidissima seedlings when 5 kg/plant of chicken manure was used as the base fertilizer at seedling transplanting period, and 29.49 g/plant of urea, 37.43 g/plant of superphosphat and 8.98 g/plant of potassium sulfate was used as topdressing fertilizer in the following growing season.

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Effects of organic fertilizer and biostimulant on soil microorganisms and tomato growth and yield

ZHAN Ji-dong, ZHI Ye, LIANG Si-wei, ZHAO Juan, LU Hao-wen, LI Tian-le

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 46-48. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.010

Abstract （489）

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This study is to explore the effects of organic fertilizer and biostimulants on soil microorganisms, plant growth and yield, and to provide the improved fertilization scheme for plant growth. Using tomato （Solanum lycopersicum） as experimental materials, the effects of different fertilization treatments were investigated by a randomized block experiment. Four treatments were set up, including control, 0.1% biostimulant, Fubang 5% organic fertilizer and Fubang 5% organic fertilizer plus 0.1% biostimulant. The changes of soil microorganisms, the plants growth and the yield of tomato in each experimental plot were recorded, and their differences were statistically analyzed. The results showed that quantity of fungi, bacteria and actinomycetes in soil were significantly increased by applying 5% organic fertilizer plus 0.1% biostimulant, and the plants growth and the yield of tomato were significantly higher than those of the other three treatments. In clude, the fertilization scheme, 5% organic fertilizer plus 0.1% biostimulant, can significantly enhance soil beneficial microbial population, promote plant growth and improve vegetable yield and quality.

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Effects of film mulching on the content of soil nutrient and production and quality of Brassica pekinensis

LIAN Zhi-cheng, HUANG Xiang, HONG Juan, CHEN Gang, DU Lei, WANG Su-ping, ZHANG-Gui you, ZHANG Li-hong, YE Li-xia

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 49-54. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.011

Abstract （381）

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A split-block field experiment was conducted with film mulching（M） or no film mulching（NM） as main factor and different fertilizer as the deputy factor（no fertilizer CK,farmer accustomed fertilization CF,reduced rate of fertilization RF and improved reduced rate of fertilization IRF）. Meanwhile, the contents of available N, available P, available K, available Zn and available B in different soil layers were determined. The results showed that the weight per plant, the biological yield, the economic yield, the fertilizer use efficiency, and the economic yield of Brassica pekinensis were significantly increased by mulching compared with no mulching. Fertilization can also significantly promote the growth of Brassica pekinensis, improve plant nutrient content and uptake. Fertilization can significantly increased nitrate content, decreased soluble sugar content and vitamin C content of Brassica pekinensis, but the effect of film mulching on quality index was not significant. Film mulching significantly increased the content of available N and available K, decreased the leaching degree of available P, and significantly affected the contents of available B and available Zn in topsoil, but not in deep soil.

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Effects of microbial agents and organic fertilizers on microbial flora in tobacco planting soil

XIONG Wei-liang, ZHANG Zong-jin, ZHANG Yuan, CAI Yan, YE Qin-xin, GOU Xiao-mei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 55-58. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.012

Abstract （439）

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In order to study the effects of the combination of microbial agents and organic fertilizers on the microbial flora of tobacco planting soil, through field plot experiments and two factors and complete combination, the number of bacteria, actinomycetes and fungi in tobacco planting soil of different treatments at different periods were determined. The results showed that,in all combinations, the number of soil bacteria at the three periods of flue-cured tobacco was the highest under the combination of microbial agent and organic fertilizers of amino acid organic fertilizer, microbial residue and shrimp peptide organic fertilizer, respectively, especially amino acid organic fertilizer compounding treatment, which was 249.1%~705.5% higher than that of other compounding treatment; The number of soil actinomycetes was optimally under the combination of microbial agents oil dry, amino acid organic fertilizer and shrimp organic fertilizer, respectively, especially at vigorious growth period, which was 1.2%~116.6% higher than that of other treatments; The number of soil fungi at the sapling stage and mature period of flue-cured tobacco was the lowest with fulvic acid organic fertilizer, and was the lowest at vigorious growth with cow manure compound treatment. In summary, the combination of microbial agent and amino acid organic fertilizer can effectively improve the microbial flora of planting tobacco soil, which is the best combination of microbial agent and organic fertilizer.

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Effects of vegetable waste compost on soil fertility and enzyme activity

LI Jian, QIAO Wei-hua, WANG Tao, AO Yan-song

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 59-63. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.013

Abstract （415）

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The effects of four different organic fertilizer treatments, commercial organic fertilizer, vegetable waste compost, commercial organic fertilizer + microbial inoculum, vegetable waste compost + microbial inoculum on the physicochemical properties and catalase of vegetable greenhouse soil were studied. The results show that each treatment can significantly reduce soil pH, increase soil organic matter content and cation exchange capacity, reduce soil bulk density and increase soil porosity to a certain extent, and the effect of adding microbial inoculants is more obvious. The content of alkali-hydrolyzed nitrogen in each treatment increased significantly, and the content of available phosphorus and available potassium increased to varying degrees. The effect of compost treatment of vegetable waste was better. Each treatment can increase the content of catalase in the soil. The composting effect of vegetable waste is better than commercial organic fertilizer. The effect of composting vegetable waste compost with microbial inoculum is better. Each treatment can effectively improve the physical and chemical properties of the soil and increase the biological activity of the soil. The effect on the soil from strong to weak is vegetable waste compost + microbial inoculum, commercial organic fertilizer + microbial inoculum, vegetable waste compost, and commercial organic fertilizer.

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Analysis and evaluation of fertilizer fermented from spent mushroom substrate of Pleurotus eryngii Pholiotanameko

JIANG Qiong-feng, LU Zhi-hong, REN Chao-di, YUAN Zhi-hui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 64-67. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.014

Abstract （387）

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To provide a theoretical basis for the utilization of bacterial chaff to make fertilizer for agricultural use and realize the reuse of waste resources, the nutrient composition and heavy metals content of spent mushroom substrate of Pleurotus eryngii Pholiotanameko fermented fertilizer was analyzed, and the influence of different fertilizers on the growth of cash crops was evaluated. The results showed that the heavy metal content of spent mushroom substrate conformed to the relevant requirements of industry standard NY 525—2012. The crude protein and crude fiber content decreased significantly after fermentation, while the content of amino acid and organic matter were increased greatly. Compared with the blank control, the mixed fertilizer were applied to cash crops ginger （Alpinia coriandriodora D. Fang）, corn （Zea mays L.） and Chinese cabbage （Brassica chinensis L.） could increase the leaf length, plant height, stem diameter, chlorophyll content, POD activity and soluble protein conten.

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Analysis of spatial and temporal differences of nitrogen and phosphorus contents in agricultural soil

SHEN Jie-yu, YANG Si-yu, HU Ai-ling, XU Feng, HUANG Yun-xin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 68-71. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.015

Abstract （411）

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Nitrogen and phosphorus emission from farmland is one of the important sources of agricultural non-point source pollution, and its emission potential may be affected by many environmental factors. Xihuang village, Xinzhou district, Wuhan city, Hubei province was selected as the experimental site, and the boxplot method and the analysis of variance method were used for the study. The results showed that the average nitrogen content of the study site was between 0.974~1.297 g/kg, and the autumn paddy field content was relatively high, while the summer dryland content was relatively low. The average level of soil phosphorus content was between 0.491 g/kg and 0.676 g/kg, among which the content of near-residential areas in summer dry land was relatively high, while that in distant paddy fields in autumn was relatively low. There was a significant difference in nitrogen content between seasons and water and drought, and there was no significant difference between the neighborhoods. There was a significant difference in phosphorus content between seasons, water and drought, and residential areas.

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An investigation on soil nutrient status and water content in Ziziphus jujuba cv. Lingwuchangzao orchards of main producing area in Ningxia

LI Hui, LI Bai-yun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 72-76. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.016

Abstract （396）

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To understand the soil nutrient and water status of main producing area of Ziziphus jujuba cv.Lingwuchangzao in Ningxia, six representative planting bases were selected to analyze the content of pH, total salt, organic matter, alkali-hydrolyzed N, available P, available K and water. Results showed that the average pH, total salt,organic matter, alkali-hydrolyzed N, available P, available K of jujube orchards soil were 8.36,1.46 g/kg, 6.68 g/kg, 47.83 mg/kg, 21.07 mg/kg and 127.61 mg/kg respectively. Compared with the 2nd National Soil Survey, the organic matter and alkali-hydrolyzed N content were lack, the available P was very abundant, the available K was abundant. The nutrient status of Orchard Village and Daquan forest farm were better. In terms of soil water content, the Orchard Village was higher, the Lyuyuanheng and Zhongxi Company were lower. The development of soil nutrient and water content in different bases were unbalanced.

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Economic and environmental benefits of maize intercropping patterns on sloping farmland in Danjiangkou reservior area

LI Tao, ZHE Zheng-hua, CAI Jian-bo, XIA Wen-jian, XUE Li-hong, YU Wen-chang, LIN Shan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 77-81. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.017

Abstract （387）

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The experiment was conducted in natural rainfall condition under in the Danjiangkou reservoir area from 2013 to 2015 to study the characteristics of nitrogen and phosphorus loss from slop farmland and economic benefit under different planting patterns of maize（Zea mays L.） monoculture, maize intercropping angelica dahurica（Angelica dahurica） and maize intercropping sweet potato [Dioscorea esculenta （Lour.） Burkill]. The result showed that the maize and other crops intercropping had better interception effect on nitrogen and phosphorus runoff loss, and could decrease the soil and water runoff loss from slop farmland. In comparison with the maize monoculture, runoff loss of total nitrogen was decreased under maize intercropping angelica dahurica and maize intercropping sweet potato, with the decreases of 32.09% and 20.37%, respectively, the runoff loss of total phosphorus decreased by 32.08% and 18.40%, respectively, and the sediment particle losses decreased by 9.18% and 11.05%. The maize intercropping angelica dahurica had the highest economic benefit, followed by the maize intercropping sweet potato, and the economic net income reached 4.24 times and 2.73 times than maize monoculture, respectively. Maize intercropping angelica dahurica was the optimal cropping pattern and should be adapted to a larger area in Danjiangkou reservoir area according to its economic benefic and non-point source pollution control by nitrogen and phosphorus in farmland.

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Climate quality suitability evaluation techniques for crisp pears in Ningling county, Shangqiu

SHI Gui-fen, ZHANG Yuan-da

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 82-87. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.018

Abstract （433）

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Using meteorological data and data of the development period of crisp pears in Ningling county, taking the climate suitability divisions that affect the quality of crisp pears, the meteorological conditions for annual crisp pear growth, and the meteorological disasters during the growing period as the main evaluation indicators, a comprehensive analysis and evaluation were carried out and weighted scores were given, and a climate quality suitability evaluation scoring algorithm was established. Statistical analysis was performed on the climatic resources and main meteorological disasters of various growth periods of crisp pear in Henan province Ningling county demonstration park in 2017, and score calculation was performed. The results show that the climate resources during the key growth period of this year can meet the growth of fruit trees and fruits. There are sufficient light and heat resources, sufficient precipitation during the young fruit period and fruit expansion period, and the main meteorological disasters are high temperature heat damage and drought in midsummer. The final score was 94.5 points, and the climate quality of the crisp pears in this area was considered to be excellent that year.

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Climate suitability assessment of summer maize in Cangzhou city

GAO Cao, HAN Dian-chen, ZHAO Xiao-han, XING Li-zhu, LI Lian-xiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 88-91. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.019

Abstract （461）

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Based on the growth environment requirements of summer maize and the climate conditions of Cangzhou city, a climate suitability evaluation model of summer maize was established, which was affected by the average temperature, active accumulated temperature, sunshine hours and precipitation during the whole growth period of summer maize. The meteorological observation data of Cangzhou city from 2010 to 2017 were selected, and the climate suitability of summer maize in Cangzhou city was improved by using GIS analysis technology and weighted comprehensive evaluation method. The regionalization study was carried out. The results showed that the distribution level of planting suitability of summer maize in Cangzhou was not uniform. The overall trend is gradually increasing from northwest to southeast. Renqiu, northwest Hejian and Suning are sub-optimal areas with relatively low economic returns. Wuqiao, Dongguang, Nanpi, Yanshan, Haixing, Mengcun and southeastern Cangxian are the most suitable areas for summer maize cultivation. Both heat and rainfall and water conditions can meet the growth needs of summer maize, and the economic returns are relatively high. It is suggested that the appropriate climatic strips should be rationally utilized. We should increase the planting area of summer maize in these areas to promote local agricultural development.

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Spatio-temporal analysis and obstacle diagnosis on water resources coordination in the middle reaches of Yangtze river

GUO Jia-li, YU Jing, HE Yu-jie, JIANG Lu, ZHANG Ze-wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 92-98. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.020

Abstract （506）

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As an indispensable natural resource for human survival and development, the rational coordination use of water resources is the main point to achieve sustainable development. In this paper, the spatial and temporal pattern of the coupling relationships among water resources, economic society, ecological environment and smart technology system in 12 cities in Hubei province from 2008 to 2017 were analyzed by extended coupling coordination degree and obstacle degree model. The results showed that the coupling coordination degree presented the significant rise-fall-up trend during the evaluation period. The current average coordination degree is 0.635 5 and in a moderate coordination level. In the spatial distribution, the coordination degree presented three evolution states. The coordination degree in the southeast area of China is the highest, while in the western area it is steadily rising, and in the central-north area it fluctuates greatly. The system obstacles are ranked as water resources, smart technology, ecological environment and economic society. The southeast and southwest areas belong to smart technology lagged, and the northwest area belongs to water resources lagged.

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Spatial-temporal distribution and variation tendency on static and dynamic agricultural ecosystem services value based on the clustering analysis in Gansu province

LIU Yang, ZHANG Wei, ZHANG Gao-sen, ZHANG Bing-lin, JIANG You-jin, ZHANG Song-lin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 99-108. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.021

Abstract （370）

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The composition of agricultural ecological services static value and different types of ecological service functions static value in Gansu province from 2008 to 2017 was estimated by the ecological service function value equivalent factor method. In consideration of the spatial dynamics character of agricultural ecological services, the dynamic value composition of various ecological services was estimated by the weight ratio assignment of different service functions based on clustering and stratification and priority index. And the temporal-spatial distribution of the dynamic value of agro-ecological services in Gansu province was studied. The results showed that the dynamic value of agricultural ecological service functions in Gansu province increased by years from 2008 to 2017. Different dynamic value contributions varied greatly. Grain conservation and other service functions had less correlation with the dynamic value. Total static value of agricultural ecological services in Gansu province from 2008 to 2017 reached 2 187.34 billion yuan. The total dynamic value was 18 34.58 billion yuan. Compared with static value, the value of each dynamic function reduced. The results of variation trend of dynamic value predicted with hots-colds pots found that changes in the agro-ecological environment in Gansu province were instability. The composition of service functions was unbalanced, as well as the elasticity was poor and restorative force was weak. To sum up, Gansu province is in urgent need of the construction of agricultural ecological service functions vigorously developing agricultural economy, actively constructing ecological agriculture, in order to maintain the balance of agricultural ecology, promote the sustainable development of agricultural ecosystems, and increase the value of agricultural ecosystem services．

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Temporal and spatial characteristics and its dynamic analysis on plant cover in Enshi city

YU Ke-jin, SONG E-ping, TAN Zhi-zhong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 109-112. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.022

Abstract （495）

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In this paper, the temporal and spatial variation and spatial distribution of plant coverage in Enshi were analyzed by transfer matrix and statistical analysis based the plant cover data which extracted from the remote sensing images during 2007 to 2015 by using RS and GIS technology and using the normalized vegetation index and pixel dichotomy model. The comparison analysis showed that the plant coverage in Enshi City was mainly of the plant coverage over comparative high level, and the proportion of the plant coverage over the third was more than 68%. The overall distribution was characterized by lower plant coverage in the central and north-eastern regions, and gradual increasing to the south-west and the north-west. The highly plant-covered area was mainly in the south-west and the south-east. The plant coverage of Enshi showed positive growth trend during 2007 to 2015 and has no degradation of partial and overall. The transfer-out area of medium and the comparative higher plant cover was basically shifted to the highly plant covered area. The area of the plant highly covered was increased by a total of 910.3 km², the increasing proportion was 22.98%. The plant coverage of most area in Enshi was at a high level of coverage, and presented growth trend in total from 2007 to 2015.

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Spatial-temporal variation analysis on land use in fuhai county

ZONG Ya-fei, CAO Yue-e

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 113-116. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.023

Abstract （393）

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Research on land use variation is a key content related to regional sustainable economic development. In this paper the variations of land use in Fuhai county including land-use variation, land-use degree were analyzed by land-use transfer matrix with the help of geographic information system and based on land-use data of Fuhai County, Xinjiang in 2010, 2012, 2014, and 2016. The results showed that the variation of land use types in Fuhai county was not significant from 2010 to 2016, and the natural state was relatively stable in whole.

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Research on low temperature and freeze injury indicators for brick wall greenhouse tomato in Inner Mongolia

XIAO Fang, LI Yun-peng, YANG Yu-hui, WU Hao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 117-120. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.024

Abstract （359）

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Based on microclimate observed meteorological data within a greenhouse from December 2017 to February 2018 in Inner Mongolia, the tomato growth data and frost damage factors were analyzed, and the regression models of daily minimum temperature inside and outside greenhouse under different weather conditions were established. Meanwhile, the minimum temperature index of different levels for tomato freeze injury was ascertained. The results showed that, the occurrence of low temperature and freeze injury for greenhouse tomato was not only related to the daily minimum temperature, but also to the duration of low temperature and relative humidity. The quadratic curve of daily minimum temperature inside and outside the greenhouse was fitted the best in clear,cloudy and overcast days,and the correlation coefficients were 0.733, 0.820, and 0.423, respectively. The temperature forecast index for mild freeze injury was -18.5~-10.4 ℃, -10.6~-7.4℃, and -8.4~-4.4℃ in clear,cloudy and overcast days, the temperature forecast index for moderate freeze injury was -20.5~-18.5 ℃, -13.0~-10.6 ℃, and -11.4~-8.4 ℃ in clear,cloudy and overcast days, and the corresponding values for severe freeze injury were ≤-20.5 ℃, ≤-13.0 ℃, and ≤-11.4 ℃ in three weather conditions, respectively.

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Study on discrimination index of winter precipitation types in Hangzhou city

LI Jin, MAO Ze-jian, ZHOU Juan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 121-125. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.025

Abstract （331）

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Based on the daily radiosonde data and surface observation data in winter during 2008 and 2017 in Hangzhou city, the indices on temperature and thickness closely related to transformation of precipitation phase are studied. Eventually, a set of objective discrimination criterion for different precipitation types is summarized in Hangzhou city. The test results show that the objective discrimination criterion has a good performance to rain and snow, and there is neither empty report nor missing report with TS scores of 100%。 In practical application, while weather factors are inconsistent with thresholds of some indices in this discrimination criterion, the supplementary reference criterions proposed in this paper can be combined. Results from this paper can provide a reference for the daily operational forecasting of Hangzhou city in winter.

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Study on the meteorological insurance index of rice insurance： A case study of Lianyungang city

DONG Jing-ming, ZHANG Yin-yi, CHEN Jiang-wen, HAO Ling, SHI Da-wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 126-130. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.026

Abstract （447）

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Based on the rice yield per unit area in Lianyungang in the past 30 years and the meteorological elements in the past 30 years, using the 5-year linear sliding average, the meteorological yield was separated, the correlation between meteorological yield and various meteorological factors was tested. According to the correlation results, sunshine was selected as the agrometeorological insurance index factor, and the most sensitive period of heading, flowering and grain-filling maturity of rice were selected, at the same time, the relationship model between the yield reduction rate and the duration of low illumination was established. Then, based on the sunshine meteorological data of each district and county in Lianyungang, the Weibull distribution model was used to simulate the probability of the occurrence of the under-illumination meteorological disaster in different areas, and the yield reduction model was used to determine the pure premium rate under different disaster triggering levels. The results showed that the yield reduction rate of rice in Lianyungang was a function of three times with the number of days of low illumination, among which the probability of occurrence of low illumination disaster was higher in irrigated Guanyun, Guannan and lowest in Ganyu district, and the pure premium rate of meteorological disaster index insurance in Lianyungang was higher in south than in north, guannan County has the highest net rate, at 8.32 per cent on the light disaster payment trigger scale, while Ganyu district has the lowest, at 2.98%. The results of this study can provide scientific basis for the development of policy-based meteorological index insurance for rice in Lianyungang.

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Study on thunderstorm potential forecast in Tangshan city

CHAI Rui, CAO Xiao-xia, WANG Ai-jun, WANG Guan, MIAO Guo-rong, XU Jian-peng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 131-135. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.027

Abstract （449）

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Combining the 552 radio sound observations during June and August from 2015 to 2017 with the lightning location information within a range of 50 km around Tangshan, this article applies dynamic clustering method to classify thunderstorm occurance in Tangshan district into three categories （as category 1, 2, 3）. The reliability of the categories is verified with overlay of lightning location information onto radar echo PPI. Ten convective parameters associated with each radio sound observation are filtrated by the means of gradual selection and stepwise regression method. The convective parameters, which are closely related to thunderstorms, are selected as the forecasting parameters and are used for principal component analysis so that an explanation on the relationship between the factors and thunderstorm is given. Finally for the potential prediction of thunderstorms, Bayes discriminant and Logistic regression discriminant are deployed to establish prediction models and Logistic regression discriminant can also provide the probability of the three categories. The results show that the forecast accuracy of the methods mentioned above are better than 70 percent and might be used as a reference in the specified district.

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Effects of mineral curing agents on absorption and enrichment of heavy metals in vegetables and pollution risk

SU Shi-bo, XU Hui-ting, JIA Fei, CHEN Huan-yuan, ZHANG Wei-wen, ZHANG Hang-jun, ZHU WEI-qin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 136-141. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.028

Abstract （403）

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Taking heavy metal contaminated soil in Huzhou, Zhejiang province, as test soil, apply 1.0% and 2.5% of 4 kinds of mineral curing agents, zeolite, bentonite, diatomaceous earth and vermiculite, setting no added blank （CK） and base fertilizer added blank （FZ） for potting test, the effects of mineral curing agents on the growth, heavy metal content and enrichment characteristics of vegetables （Shanghai Green） were studied; and pollution assessment and health risk assessment of heavy metals were carried out. The results showed that the addition of mineral curing agent can effectively promote the growth of vegetables and reduce the heavy metal content in edible parts, and can reduce the absorption and accumulation of Cu, Zn, and Cd in the soil by vegetable roots and stems and leaves. After the addition of mineral curing agent, the comprehensive pollution level of heavy metals in vegetables can be reduced to mild pollution or alert level, and the health risk index has also decreased. With the addition of 2.5% vermiculite, the overall pollution level and health risk are the lowest. The addition of appropriate amount of mineral curing agent can promote the growth of vegetables in contaminated soil and control the pollution and health risks of heavy metals.

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Analysis of soil pollution and chemical oxidation remediation in relocated chemical sites

ZHOU Huan, HU Lin-chao, HU Min, ZHU Lei-ming, MAO Lin-qiang, ZHANG Wen-yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 142-146. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.029

Abstract （434）

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Aiming at the pollution problem of the site left after the relocation of chemical companies, a contaminated soil at a relocated chemical site in southern Jiangsu was taken as the research object. The status quo analysis determined that the target pollutants were polycyclic aromatic hydrocarbons, volatile organic compounds and chlorobenzenes. According to the characteristics of organic compound polluted sites, 1-naphthylamine, 1,2-dichlorobenzene and 1,4-dichlorobenzene were used as main pollutants to remove the pollutants, and the site soil was treated with electro-oxidation in situ remediation technology. The results show that the water-soil mass ratio, electro-oxidation time, and current intensity are the main influencing factors for electro-oxidation repair. When the mass ratio of water and soil is 2∶1, the current strength of the oxidation electrode is 300 A, and the electrooxidation time is 15 min, the removal rate of the main pollutants in the soil, 1-naphthylamine, 1,2-dichlorobenzene, and 1,4-dichlorobenzene reaches more than 90%; The heavier the organic compound polluted soil, the higher the removal rate within the same electro-oxidation time interval, indicating that the method is suitable for treating soils with higher pollutant concentrations. It is estimated that it will cost about 70.9 yuan to treat 1 t of soil. This study can provide technical process parameters and theoretical basis for the remediation of organic compound contaminated sites in this chemical enterprise, and can provide technical choices and process design references for the same type of contaminated site remediation.

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Best management practices for non-point pollution prevention from orchard in Dongjiang river source areas

WANG Tao, SHI Xiao-yan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 147-150. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.030

Abstract （393）

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In order to control non-point source pollution from the development and management practice of orchard, by choosing a large-scale navel orange planting orchard as the research base, the controlling measures for non-point source pollution from orchard in Dongjiang river source were established according to the best management practices （BMPs） theory. Results revealed that, compared with that from the contrasting area （clean tillage treatment）, after taking BMPs, concentrations of TP, TN and NH₄⁺-N in runoff from testing area in the orchard were reduced by 27.78%, 57.93% and 88.89% respectively, and the interception effect of TN and NH₄⁺-N in the runoff was very significant（P<0.01）.

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Study on landscape pattern characteristics and vulnerability differences in north and south river basins of Qinling Mt.

MA Xin-ping, HAN Shen-shan, WANG Lei, WANG Jian-xing, HE Wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 151-159. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.031

Abstract （443）

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The Qinling Jinqian River Basin and Bahe River Basin were selected as the research objects, and landscape pattern analysis was used to evaluate the ecological status of the basin and interpret the ecological process. Based on the remote sensing images of 2000, 2008 and 2016 as the main information sources, the land use status and landscape pattern of the two river basins were studied. The results showed that the area of forest land, unused land and water areas in the Jinqian River Basin increased, while grassland, construction land, and cultivated land decreased. The area of forest land and construction land in the Bahe River Basin increased, and unused land, waters, cultivated land, and grassland decreased. Judging from the landscape pattern index of the two river basins, the advantage of the Jinqian River waters development is getting higher and higher, and the advantage of Bahe waters development is higher than other land types. In 2000 and 2015, the fragmentation of the landscape in the two river basins decreased, and the development of the study area tended to be concentrated. There are differences in the spatial distribution of landscape vulnerability and the distribution of each level in the two river basins. The level of landscape vulnerability is consistent with the intensity of land use. The research results can provide a scientific basis for the coordinated development of eco-economic development in the Qinling Mountain Basin.

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The effect of slim cigarette material parameters on ammonia in mainstream smoke

XIAO Ke-yi, ZHUANG Ya-dong, CHEN Kun-yan, DAI Ya, ZHANG Yan, WANG Chang-guo

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 160-162. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.032

Abstract （422）

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A mathematical model and model analysis were applied to investigate the influence of slim cigarette material factors, including cigarette paper permeability（X₁）, tipping paper permeability（X₂）, plug wrapper permeability（X₃） and specification of tow at tobacco end（X₄）, on ammonia yield in cigarette mainstream smoke. The single factor effect and marginal effect were analyzed. The results showed that： ① the order of the effect of 4 factors on the ammonia yield was： X₂>X₃>X₄>X₁; ② the optimized material combination for low ammonia delivery were： X₁=70 CU,X₂=800 CU,X₃=6 000 CU,X₄=5 303.3 I.

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Study on the process of liquid smoked sturgeon slices by dipping method

YANG Xing, ZHANG Mei-yan, LI Xiao-yi, CAI RANG Zhuo-ma, ZHAO Fei, YANG Ming-ju

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 163-166. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.033

Abstract （378）

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This study optimized the processing procedure of liquid-smoked sturgeon slices. Sensory evaluation was performed on the test samples,and the effects of salt concentration,curing time,fumigation time,liquid fumigation time and drying time on product quality were also studied by multifactor and orthogonal experiments. Determined the optimal process parameters： salt concentration 3%,curing time 3 h,smoked liquid concentration 3%,liquid smoked time 60 min,60 ℃ under the condition of hot air circulation drying 120 min,under the condition of 90 ℃ baking.

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Study on technology of quercetin inclusion compound and jelly

QIU Zhen-yang, LIANG Zhong-huan, BAI Si-xiao, WANG Shu-min, SU Li-jie, CHEN Xiao-yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 167-173. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.034

Abstract （404）

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Quercetin hydroxypropyl-beta-cyclodextrin （Quercetin HP-β-CD） inclusion complex was prepared by solution stirring method. The inclusion conditions were optimized by Box-behnken response surface methodology with the inclusion rate as the evaluation index. Quercetin HP-β-CD was used as the main raw material to prepare a new type of quercetin jelly. The optimum formula was optimized by orthogonal experiment. The results showed that when the ratio of solid to liquid was 1.4∶1.0 （mg/mL）, the embedding temperature was 60 °C, and the embedding time was 122 min, the inclusion ratio of quercetin embedding rate was 66.03%. And the best formula for jelly was quercetin inclusion compound was 1%, and the ratio of carrageenan, konjac gum and xanthan gum in the compound gel was 5∶4∶1, the total gum content was 1.2%, the sugar addition was 20%, and the citric acid addition was 0.10%. The jelly prepared by this formula has uniform color, smooth and delicious, unique flavor of quercetin, as well as certain nutritional value and health care effect.

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Optimization of extraction conditions of coix polysaccharide and its inoxidizability and transdermal absorption

LI Shu-ping, PENG Jun-chao, LI Meng, AN Quan, WANG Chang-tao, ZHAO Dan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 174-179. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.035

Abstract （436）

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To optimize the extraction and fermentation conditions of the polysaccharide of Coix kernel And studied its antioxidation and transdermal absorption effect, which provided reference for its application in anti-aging and transdermal absorption of human body. Results showed that the optimum conditions for the water extraction of Coix polysaccharide were 0.11, the temperature was 90 ℃, the time was 7; the best fermentation condition was Saccharomyces cerevisiae, the material liquid was 0.1, the time 54h,PH was 4.5; the extraction rate of coix polysaccharide was about 3 times of water. The antioxidant resistance of coix polysaccharide fermentation broth is better than that of water extract. The 24 h transmittance of coix polysaccharide fermentation broth was 1.14 mg/cm², and the 24h transmittance of water extract was 0.53 mg/cm².

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Research on microbial fermentation of Tamarindus indica L. and its application in tobacco

WU Ming-mei, LI Xian, ZHENG Lin, JIANG Mei-hong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 180-183. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.036

Abstract （400）

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The extract of Tamarindus indica L. was carried out by method of microbial fermentation, and the fermentation process was optimized with SPSS Software. The fermentative extract was applied in cigarette as a flavor. The research result showed that after microorganism treatment, the special fermentation aroma was increased. Besides soft and delicate aroma, the sweetness was increased, the stimulation was reduced, and the improvement of tobacco aroma was more obvious. The species and content of volatile components increased.

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Isolation and purification of yeast polypeptides and efficacy of cosmetics

FU Hao, LIU Ping-ping, ZHU Jian-feng, LI Yan-long, LI Meng, WANG Chang-tao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 184-187. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.037

Abstract （517）

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yeast protein was extracted from yeast cells by means of repeated freeze-thaw and ultrasonic crushing. The supernatant of yeast fermentation and yeast cell fluid were purified by isoelectric point precipitation method. Crude protein was obtained, and the antioxidant capacity of protein and tyrosinase inhibition ability were studied. The results showed that the supernatant of saccharomyces cerevisiae, yellow wine yeast and sake yeast had good DPPH scavenging ability, and the concentration of 2.6g /L could reach 72%. The supernatant of saccharomyces cerevisiae among the three yeast supernatant proteins had better inhibitory effect on tyrosinase. The three yeast crude proteins basically had no DPPH free radical scavenging ability at about 0.45g /L, and the inhibition rate of saccharomyces cerevisiae crude proteins on tyrosinase was better than that of other yeast crude proteins. The DPPH radical scavenging rate and tyrosinase inhibition rate of 3 kinds of yeast polypeptides with 3.5 g/L were about 50% and 12% respectively.

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Determination of chlorogenic acid and caffeic acid in Vernonia patula Merr by HPLC

GUO Hai-jiao, LIU Jin-bao, HU Hua, WANG Xian-jun, LIU Wen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 188-190. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.038

Abstract （409）

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To establish a method for the determination of caffeic acid and chlorogenic acid Vernonia patula by HPLC. The separation was performed on Welch Ultimate XB-C18（4.6 mm×250 mm,5 μm）with the acetonitrile-0.2% phosphoric acid aqueous solution as the mobile phase with gradient elution. The volume flow rate was 1.0 mL/min. The detection wavelength was 327 nm. The column temperature was 25 ℃. The linear range of caffeic acid and chlorogenic acid were 47.84~239.2 μg/mL（R=0.999 8）、10.05~50.25 μg/mL（R=0.999 9）, and the average recoveries （n=6） were 96.69%（RSD=0.74%）、102.10%（RSD=2.00%）. This paper established a method for the contents of chlorogenic acid and caffeic acid in Vernonia patula. The method is accurate, sensitive, credible and repeatable. It can be applied to the quality control of Vernonia patula.

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GC-MS analysis of chemical constituents of volatile Oil from different area of Euonymus fortune in Guangxi

LIANG Shuang, LU Sen-hua, WU Xiu-cai, HE Li-hong, HUANG Jian-jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 191-195. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.039

Abstract （471）

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The chemical constituents of the essential oil of Euonymus fortune from different areas were compared and analyzed by GC-MS. The results showed that 25 chromatographic peaks were isolated and 13 chemical constituents were identified in Binyang village, accounting for 98.24% of the total volatile oil. 29 chromatographic peaks were isolated from Rong village, and 14 chemical constituents were identified, accounting for 89.96% of the total volatile oil. 32 chromatographic peaks were isolated from Mengshan village, and 12 chemical constituents were identified, accounting for 92.16% of the total volatile oil. 28 chromatographic peaks were isolated from Rong village, and 11 chemical constituents were identified, accounting for 84.79% of the total volatile oil. Five of the chemical constituents of the volatile oil from the three places of origin are common to the four. They are phytol,palmitic acid,n-nonane,cumene,hexahydrofarnesyl acetone. It can be seen that the main components of volatile oil from different producing areas are different.

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Study on gold immunochromatography assay for rapid detection of cypermethrin

WU Xiao-sheng, JIA Fang-fang, CUI Hai-feng, WANG Zhao-qin, WEI Li-jie, CUI Na, ZHAO Zheng-miao, WAN Yu-ping

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 196-198. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.040

Abstract （396）

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To establish a rapid assay for detection of cypermethrin in vegetables and fruit， and gold immunochromatography assay was studied. The limit of detection is 1 μg/kg. And we can obtain the results in 15 minutes. The assay gives no false positive and false negative results. The method is accurate， simple， reliable and convenient to rapidly detect cypermethrinon the spot.

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Application of a migration learning algorithm in tomato disease detection

KONG De-feng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 199-203. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.041

Abstract （511）

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An image classification algorithm based on Inception-v3 and migration learning is proposed to solve the problem that the current intelligent identification of tomato diseases is not accurate and time-consuming. Eight tomato disease leaves and healthy leaves were collected from the experimental field, the images were scanned into images using a 10 megapixel HD scanner, the images were classified into 9 folders, and the blade attributes were manually labeled. Finally, based on the Inception-v3 model combining migration learning algorithms to classify test healthy and diseased leaves, and compare them with traditional image classification algorithms （KNN、 SVM、 BP neural network） and non-migration learning algorithms. The experimental results show that, based on Inception-v3 model combined with migration learning algorithm can quickly and effectively identify grow healthy tomato and diseased tomato in tomato disease image classification, and can identify tomato disease types efficiently. Among them, the classification accuracy of health and disease is 0.976 0, and the average accuracy of disease types is 0.929 7, which provides a certain degree of support for tomato disease detection and prevention.

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A fast approach for ortho image generation of the search and resue region based on unmanned aerial vehicle system

LIN Huo-duan, ZHANG Zhi-lin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 204-206. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.042

Abstract （371）

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In the emergency rescue, it is necessary to quickly understand the actual situation of the search and rescue region. A fast approach for ortho image generation of the search and rescue region based on unmanned aerial vehicle system was put forward, through image sequential adjustment, ortho rectification based on CPU-GPU, and fast image mosaic, ortho image of the search and rescue region without-obvious seam-line was obtained.

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The information analysis of SSR loci in the red claw crayfish（Cherax quadricarinatus） transcriptome

LI Xi-lian, GUO Jian-lin, LI Qian, SHI Wei-da, HUANG Zhen-yuan, GU Zhi-min

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 207-211. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.043

Abstract （532）

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The transcriptome sequencing data of the red claw crayfish （Cherax quadricarinatus） was analyzed, and the primers were developed by PCR amplification and capillary electrophoresis, the primer polymorphism and versatility analysis were carried out. 22,727 simple repeat （SSR） sites were detected from 67,369 Unigenes, and functional annotations were performed on Unigenes containing SSR sequences. The results show that, the microsatellite sequences in the transcriptome of the red claw crayfish are abundant, and the dinucleotide and trinucleotide repeat types appear more frequently, accounting for 52.67% and 44.25% of the total SSR frequency, respectively; the tetranucleotide pentanucleotide and hexanucleotide repeat types appeared less frequently, accounting for 2.62%、 0.23% and 0.24% of the total SSR frequency, respectively. There are 68 SSR repeat types, and the number of repetitions ranges from 5 to 84.5 pairs of SSR primers were screened, and 60 samples from Zhejiang and Taiwan were analyzed for genetic diversity, the average polymorphic fragment per every prime was 5.6. The results of this study lay the foundation for the in-depth development of functional SSR markers of red claw crayfish, and also support the molecular marker-assisted breeding of red claw crayfish.

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Analysis of molecular characteristics and receptor-binding ability of low pathogenic and highly pathogenic H7N9 AIV subtype avian influenza virus

XUE Yu-han, REN Zhu, SHANG Yu, TANG Guo-yi, JIA Miao-miao, LU Yue-feng, LI Qiong-qiong, LI Hui-min, LUO Qing-ping, WEN Guo-yuan, PAN Zi-shu, ZHAO Zong-zheng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (7): 212-217. DOI: 10.14088/j.cnki.issn0439-8114.2020.07.044

Abstract （416）

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Two H7N9 subtype avian influenza viruses （AIV） isolated from chicken pathological material in Hubei Province, A/chicken/Hubei/093/2017（H7N9） and A/chicken/Hubei/207/2017（H7N9）（with CK93 and CK207 as their abbreviation, respectively） were analyzed for whole-genome sequencing. The hemagglutinin （HA） and neuraminidase （NA） sequence conservation, as well as HA glycosylation site, NA resistance site and key amino acid sites of 6 internal gene-encoded proteins were analyzed, meanwhile, the receptor binding ability of the two strains was determined as well. The results suggested that the HA protein cleavage site of CK93 was PEIPKGR↓GLF, which indicated a low pathogenicity of CK93, whereas CK207 was mutated to PKPKRTAR↓GLF, which indicated a high pathogenicity of CK207. At the same time, the 226 amino acid sequence of HA in CK93 was Leucine （226L）, while its counterpart in CK207 was Glutamine （226Q）. However, the receptor binding ability results suggested that two isolates can be bound to both α-2,3 sialic acid receptor and α-2,6 sialic acid receptor, it is indicated that the Q226L is not a decisive factor affecting receptor binding characteristics. In light of the above information, it is suggested that precaution and control to the mutation of H7N9 subtype avian influenza should be enhanced in order to lower any risk of epidemic owing to its mutation.

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Application of mass spectrometry in the study of plant proteome

YAN Xin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 5-6. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.001

Abstract （455）

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Plant proteome reveals the dynamic change of physiological characteristics and plays an important role in multiple biological and pathological processes, including growth and development of plants, tissue differentiation and resisting external interferences. The advances in proteomics of plant different organs, different development stages, response to biotic and abiotic stresses based on mass spectrometry are reviewed, in order to provide reference for the design of train of thought and experimental method for the follow-up study of plant proteome.

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The structure of Argonaute protein and research progress in plants

GU Shao-wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 11-16. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.002

Abstract （842）

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In eukaryotic cells, many small non-coding RNAs combind with Argonaute protein to form the RNA-induced silencing complex, the Argonaute-sRNA can regulate gene expression by recognizing complementary RNA targets, which is called RNA interference. Argonaute-sRNA complexcan play interfering roles via multiply mechanisms in plants, and then participate in key biological processes such as pathogen defense and developmental regulation. Here, we review the classification and structural characteristics of Argonaute protein, as well as mechanisms of RNA interference and biological functions on Argonaute in plants.

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Current status and development prospect of upland rice planting in Hubei province

LI Ping, CAO Peng, JIANG Song-song, ZHANG Jian-she, CAO Cou-gui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 17-20. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.003

Abstract （511）

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With the increasing shortage of water resource, there is a urgent demand to develop upland rice planting in Hubei province. The significance of developing upland rice production in Hubei province is elaborated, the current status of upland rice planting is introduced, the remaining problems are analyzed, and the development prospect of upland rice production for Hubei province is clarified.

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Effects of nitrogen on growth and physiological characteristics of wheat seedlings under drought stress

WU Xiu-ning, LIU Ying, WANG Xin-jun, ZHAO Zhi-xin, ZHAO Peng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 21-24. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.004

Abstract （503）

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The changes of growth indexes, physiological characteristics and chlorophyll content in wheat seedling leaves under different water-nitrogen coupling were determined. The wheat cultivars, namely Shangmai 5226 and Xiaoyan 15, were treated with 3 nitrogen levels low nitrogen, medium nitrogen, high nitrogen（0.5、8.0、15.0 mmol/L） and drought treatment simulated by PEG-6000. The results showed that, under moderate drought stress, maximum root length, net photosynthetic rate and chlorophyll content were high nitrogen > medium nitrogen > low nitrogen, seedling height and stomatal conductance were medium nitrogen > high nitrogen > low nitrogen; under severe drought stress, maximum root length showed low nitrogen>medium nitrogen>high nitrogen, net photosynthetic rate and chlorophyll content were medium nitrogen>high nitrogen>low nitrogen; under two drought treatments, root-shoot ratio was low nitrogen > medium nitrogen > high nitrogen, and intercellular CO₂ concentration was high nitrogen > low nitrogen > medium nitrogen. Moderate nitrogen could enhance the growth and photosynthetic capacity of wheat seedlings, indicating that nitrogen had a certain compensatory effect on drought stress.

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Evaluation of land resources carrying capacity in Zhengzhou city based on remote sensing image

JIA Yi-bin, WANG Han

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 25-29. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.005

Abstract （472）

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Taking Zhengzhou city, Henan province as an example, the relevant land use status data is obtained with the help of remote sensing technology, through the Landsat TM remote sensing image processing of Zhengzhou city in 2004, 2009, and 2013, and the coordinated development of population-resource-environment is fully considered. The carrying capacity of Zhengzhou city land resources are evaluated by using the analytic hierarchy process method, objective weighting method and multi-factor weighted sum method to establish an evaluation system of land carrying capacity in Zhengzhou city. The results show that from 2004 to 2013, the carrying capacity of land resources in Zhengzhou city has increased year by year; from 2009 to 2013, the use of land resources and the allocation of population resources are uneven, and the intensity of land use is low, due to the rapid population growth of Zhengzhou city; from 2004 to 2013, the land use structure of Zhengzhou city tends to be optimized, and the efficiency of land use has increased significantly. The land resource carrying capacity of Zhengzhou city in 2013 is close to the ideal state, indicating that the capacity during this period is relatively good, but it still needs to be improved for the sustainable development of land use.

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Regional response of climate heat resources in typical oasis eco-region

WANG Yun-peng, LI Hong-ying, YU Ya-nan, LEI Shu-qin, GUO Liang-cai, HU Xin-hua

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 30-34. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.006

Abstract （419）

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Jiuquan oasis in the west of Hexi Corridor is selected as the representative. Based on the daily temperature data of 8 meteorological stations in Jiuquan city from 1961 to 2018, the temporal and spatial variation characteristics of accumulated temperature greater than or equal to 10 ℃ in typical oasis eco-region in recent 60 years was analyzed by using 5-day moving average, climate tendency rate, M-K mutation test method and wavelet analysis. The results are as follows： The accumulated temperature greater than or equal to 10 ℃ is unevenly distributed, and the regional distribution is quite different. The accumulated temperature greater than or equal to 10 ℃ shows an increasing trend in which the first day is ahead of schedule and the final day is delayed. In recent 60 years, the accumulated temperature greater than or equal to 10 ℃, the final day and the duration days was changed abruptly in 2004, 1974 and 1997, respectively. The accumulated temperature greater than or equal to 10 ℃ has a strong oscillation period of quasi-26 years, since 1961—1970, 1971—1999 and 21st century, the change process of rise-fall-rise has been generally presented; The short-period oscillation of the first day, the final day and duration days of the accumulated temperature greater than or equal to 10 ℃ are obviously different at each time interval.

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Analysis on characteristics of drought disasters and their impacts in Hubei province

ZHENG Zhi-bin, LIU Ke-qun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 35-40. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.007

Abstract （563）

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Under the background of global warming, the characteristics, spatial-temporal distribution, evolution trend and disaster-causing mechanism of drought disasters in Hubei province were analyzed. The results show that drought disasters occur in all seasons in Hubei province. Northwest Hubei including northwest, humpy ground in north, hilly area in central are the areas with the most drought disasters; While Southwest Hubei in south of the Three Gorges of Yangtze River is the area with the least drought disasters. July to October is the month with the most drought days, and January to May is the month with the least drought days. The frequency and intensity of drought disasters in Hubei province show a significant increase trend, which related to natural and geographical environment, human activities, water infrastructure construction and other factors. The threats of drought disasters to agricultural production, food security, drinking water security, ecological environment security and social development in Hubei province are becoming more and more prominent, so effective countermeasures should be taken.

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Relationship analysis between autumn precipitation characteristics and wheat yield in northwest China

LIU Ying, ZHOU Zhong-wen, QIU Ning-gang, LI Qiao-xia

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 41-44. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.008

Abstract （403）

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In order to study the spatial distribution characteristics and temporal variation of autumn precipitation in northwest China and the relationship with wheat yield, EOF and REOF were used to analyze the data of 119 meteorological stations in northwest China from 1961 to 2016. The results showed that there were significant spatial differences in autumn precipitation in northwest China. The minimum was 0.9 mm at Lenghu meteorological station in Qinghai province, and the maximum was 270.0 mm at Shiquan meteorological station in Shaanxi province. Autumn precipitation in northwest China mainly increased in the 1960s, 1970s, 1980s and 2001—2016, while decreased in the 1990s. The autumn precipitation in northwest China could be divided into six climatic zones. The precipitation evolution of each representative station showed that there were many representative stations in Urumqi, Hetian and Golmud, but little others in 1960s and 1970s. The evolution of the third-order curve showed that precipitation of Urumqi decreased after 2005, while other representative stations increased. Precipitation in autumn was positively correlated with wheat yield in the following year.

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Analysis of apple climate quality certification in Xifeng Loess Plateau in 2018

ZHOU Zhong-wen, LIU Ying, DU Jun, QIU Ning-gang, ZHANG Jun-lin, LI Qiao-xia, ZHANG Ke-xin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 45-49. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.009

Abstract （395）

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In order to study the impact of the climatic conditions of agricultural products on its quality, the observation data of light, humidity, temperature and other factors in Xifeng over the period 1981 to 2018 were compared and analyzed to determine the main climatic factors which affected apple quality during the key growth period. The meteorological indicators of key factors were quantified, and the disaster indicators of severe weather were refined and graded. According to the close relationship between the quality of agricultural products and climate, the certification model was established by weighted scoring method. Combined with the actual monitoring of weather and climate in 2018, the climate quality level of agricultural products was comprehensively assessed.

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Effcets of different LED light qualities on growth characteristics of Myriophyllum verticillatum and purification efficiency of livestock and poultry farm wastewater

HU Qing-quan, SHA Qian, YANG Ren-can, CHANG Ya-jie, HAN Min, ZHAO Zhi-yong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 50-53. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.010

Abstract （446）

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In order to construct plant factory technology for Myriophyllum verticillatum L. treatment of livestock and poultry farm wastewater, the effects of different light quality on the growth characteristics of Myriophyllum verticillatum L. and purification capacity of livestock and poultry farm wastewater were investigated. The results showed that the biomass and purification efficiency were significantly higher under the treatment of red and blue light 3∶1 than that under the treatment of red and blue light 5∶1 and white light, with no significant difference with natural light （CK）. The content of chlorophyll a, chlorophyll b and total chlorophyll under the treatment of red and blue light 3∶1 and 5∶1 were not significant compared with CK, but significant compared with the treatment of white light. The treatment of red and blue light 3∶1 was similar to natural light in biomass, purification efficiency and photosynthetic pigment content, and it was suitable for the growth of Myriophyllum verticillatum L..

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Screening of mixed sulfate reducing bacteria and study on its optimal growth conditions

PU Jia-hong, LUO Xue-gang, WANG Jing-ming, LIANG Zhu-ming

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 54-57. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.011

Abstract （480）

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The mixed sulfate-reducing bacteria were screened by selecting the medium. At the same time, the optimum growth conditions were studied. The effects of inoculation amount, temperature, initial pH and Fe²⁺ on the growth of the mixed sulfate-reducing bacteria were investigated. The results showed that the mixed sulfate-reducing bacteria grew best when the inoculation amount was 10%, the temperature was 35 °C, the initial pH was 6.5, and the Fe²⁺ in the medium was 200 mg/L.

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Study on effects of application of two biological-bacterial fertilizer on the of starch sweet-potato E6

DONG Cui, YANG Han, YANG Kang, HUANG Zhi-mou, GONG Wan-neng, CHAI Sha-sha, LEI Jian, WANG Lian-jun, SHU Wen-jin, YANG Xin-sun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 58-61. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.012

Abstract （363）

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The effects of two kinds of biological-bacterial fertilizer on the growth, yield and quality of sweet potato E6 were studied in Zhijiang, Ezhou and Xianning. The results showed that the content of nitrogen, phosphorus and potassium in the treated soil was higher than that in the control soil. The application of biological-bacterial fertilizer had no obvious promoting effect on the late yield formation of sweet potato E6, but it could significantly reduce the incidence of field diseases, especially the effect of solid bacterial fertilizer was more obvious. Sweet potato E6 had the highest yield in Zhijiang, followed by Ezhou and Xianning. In quality, Zhijiang was the best, Xianning and Ezhou were the second. The differences among the three locations were mainly due to the soil nutrient status and the influence of climate and environment. Higher potassium content and lower nitrogen content were conducive to the formation of yield.

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Fertilizer effects research of new fertilizers on pepper

LI Lan-xian, ZHANG Song-shan, ZHAO Ding-jie, YIN Ming

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 62-65. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.013

Abstract （381）

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Experimental studies were carried out on the effects of new fertilizers, including reducing fertilizer application, increasing organic fertilizer on the yield, quality and soil of pepper. The results showed that the treatment of 7 500 kg of organic fertilizer per hectare and 30% less fertilizer than normal fertilizer was more effective than that of a single chemical fertilizer, and the yield related traits such as weight increased, nitrite content was significantly reduced, vitamin C and soluble protein content was increased. Under the synergistic effect of fertilizer and organic fertilizer, the fruit characters of the compound fertilizer 1 260 kg plus microorganism organic fertilizer 7 500 kg were the best, the yield was the highest and the quality was better. Compared with single fertilizer, the yield of water-soluble fertilizer was the highest. The treatment of trace elements in application had the lowest fruit rate of malformation. Controlled release fertilizer could improve fruit quality and reduce nitrite content. In view of the problems of soil acidification and unscientific nutrient ratio after the experiment, it was suggested to apply formula fertilization scientifically.

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Optimization of pretreatment process conditions for fumaric acid wastewater by response surface methodology

HUANG Biao, FAN Cong-ying, LAN Tian-xiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 66-70. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.014

Abstract （383）

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The three-dimensional electrochemical method was used to pretreat fumaric acid wastewater, and the removal rate of ammonia nitrogen was mainly studied. Based on the BBD response surface method, the individual and interaction of voltage, reaction time and initial pH were explored, and a mathematical model of ammonia nitrogen removal rate was established. The results show that the significant order of influence factors is voltage>reaction time>initial pH, and there is a certain interaction among the three, but it is not significant. A satisfactory prediction quadratic polynomial equation was derived by response surface methodology. The maximum removal rate of ammonia nitrogen is predicted to be 88.7%. The optimum operating conditions are： The voltage is 28.41 V, the reaction time is 87.27 min, and the pH is 4.55. Using the above best conditions, the ammonia nitrogen removal rate of 87.6% was obtained, which is consistent with the predicted value of 1.2%.

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Effects of compound preparation of tribenuron and fluoroglycofen-ethyl WP on weeds control and nutrient content in wheat field

ZHU Wen-da, WU Jia-jia, LI Lin, SONG Zhao-xin, ZHU Jia-hong, YAN Dong-dong, WANG Qiu-xia, LI Yuan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 71-73. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.015

Abstract （563）

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The control effect and application safety of 20% tribenuron · fluoroglycofen-ethyl wet table powder（WP） on weeds in wheat fields were studied using field test method. The results showed that 30 days and 40 days after application, the comprehensive plant control effects of weed control varied from 75.49% to 88.93%, 59.61% to 70.42%, respectively; And the comprehensive fresh weight control effects was 81.92% to 87.29%.

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Effects of triasulfuron·isoproturon on controlling weeds and nutrient in wheat field

ZHU Wen-da, CAO Ao-cheng, WU Jia-jia, LI Lin, SONG Zhao-xin, ZHU Jia-hong, YAN Dong-dong, WANG Qiu-xia, LI Yuan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 74-76. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.016

Abstract （429）

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50% triasulfuron · isoproturon wet table powder（WP） for controlling weeds in wheat fields was tested. The results showed that 30 days and 90 days after application, the comprehensive plant control effects of weed was 96.08%~99.46%, 98.31%~100%, respectively; 90 days after application the comprehensive fresh weight control effects was 99.95%~100%, The comprehensive plant control effects of the control agent 50% ethersulfuron WP and 50% isoprolong WP on weed control were 97.37%, 97.09% and 100%, 98.99%, respectively, and the fresh weight control effects were 100% and 99.97%, respectively.

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Study on the Zhanshi biological pesticides degradation agent of five kinds of pesticide degradation effect in Solanum muricatum trees

LIU Chao, WANG Yue, QIU Bi-li, DUAN Li-na, YANG Yan, ZHANG Shao-long, MEI Li-bao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 77-79. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.017

Abstract （406）

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To analyze degradation effect of fenpropathrin, carbendazim, propiconazole, chlorpyrifos, methidathion on Solanum muricatum trees by Zhanshi biological degradation of pesticide. The pesticides with biological degradation of pesticide were used on Solanum muricatum trees, and blank control was done at the same time, the degradation effect of biological degradation of pesticide to pesticides on days 1, 5, 10 and 15. The results showed that the Zhanshi biological degradation of pesticide had degradation effect to fenpropathrin, carbendazim and propiconazole, the degradation rate was from 55.3% to 90.7%, the degradation effect was not remarkable to chlorpyrifos and methidathion, the degradation of cycle was 1~15 days, and the effect had weakened after 15 days.

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Determination of bacterial inhibitory fluid of fringe cells in pea root by several antibiotics

WANG Fang, QIAO Mei-xing, YU Min

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 80-82. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.018

Abstract （352）

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By means of liquid culture, the inhibition of 5 antibiotics on bacteria contaminated by culture medium and degradation of edge cells of pea root were determined. The results showed that the five kinds of antibiotics in 50~4 000 mg/L concentration gradient, in both the edge bacterial cell cultures of certain inhibitory effect. Among them, the appropriate selective concentrations of roxithromycin, cefuroxime octyl and enol were 2 000, 1 000 and 1 000 mg/L, respectively, and the inhibitory rates reached 93.48%, 58.70% and 91.30%, respectively. The degradation of penicillin, roxithromycin, cefuroxime octyl, gentamicin and enol at 500, 500, 1 000, 1 000 and 500 mg/L on marginal cells was slow, which was 63.89%, 58.95%, 60.80%, 73.15% and 58.95%, respectively. The effect on marginal cells was significantly enhanced with the increase of concentration.

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Summer reproductive capacity of Pomacea canaliculata in different habitats of Guangxi

LIANG Bi-xia, CHEN Xin-yu, WU Zheng-jun, CHENG Rui, HUANG Jin-long

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 83-88. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.019

Abstract （381）

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In order to understand the reproductive capacity of Pomacea canaliculata in different regions of Guangxi and its influencing factors, six habitats （rice field, waste field, gutter, pond, river, swamp） in Guilin, Laibin and Beihai in Guangxi, were collected from July to August 2018. The length, width and weight of the eggs mass and egg diameter as well as the total number of eggs were measured. The correlations between incubation rate and these factors were analysed, respectively. The results showed that the number of single egg grains was 48~1 041, and the mean value was （256.19±155.67）. The number of eggs in the pond habitat was the highest, and the mean value was （395.61±213.33）. The number of eggs in waste field was the lowest, the mean value was （168.39±55.47）; The egg diameter was 1.72~2.72 mm, mean value was （2.17±0.17） mm, and the diameter of the ditch habitat was the largest and the mean was （2.32±0.13） mm. The diameter of the egg in the rice field was the smallest, and the mean value was （2.07±0.16） mm. The number of egg grains and the diameter of egg grains in different areas were Laibin > Guilin > Beihai. The number of egg grains between habitats showed pond > river > swamp > ditch > paddy field > waste field. The diameter of the egg grains in the habitat was pond> swamp> ditch > river > waste field > rice field. Pomacea canaliculata in Guangxi in the summer was not related to latitude. The habitats with high water surface height or food were rich, and the number of eggs was larger and the diameter of the egg grains was larger. There were significant differences in egg number and egg diameter between different regions and habitats.

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Effects of heat treatment on physiological characteristics of fruits of passion fruit after harvest

TENG Zheng, YANG Cui-feng, HUANG Li-zhu, GAN Shan-ping, CHENG Qiao-zhen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 89-92. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.020

Abstract （407）

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Hot water treatment at different temperatures （40 ℃, 45 ℃, 50 ℃ and 55 ℃） for 2 min, 5 min, 8 min and 10 min on the changes of fruit firmness, soluble protein and soluble sugar content on postharvest purple passion fruit during storage were studied, in order to investigate the effects of heat treatment on postharvest passion fruit physiological traits as well as the optimal heat treatment temperature and duration. The results showed that hot water treatment at 55 ℃ for 2 min delayed the decline of fruit firmness, soluble protein and soluble sugar content during the storage of passion fruit, postponed the ripening process of passion fruit.

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Study of the suitability and supporting technology of high ridge and plastic film mulching technology for flue-cured tobacco in Chenzhou city

YANG Yang, ZHANG Shi-zhou, WAN Ding-hai, LIU Bi-rong, LIAO Jian-song, JIANG Chao-qiang, GONG Shuang, WU You-xiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 93-98. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.021

Abstract （420）

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Using the low ridge and no plastic film mulching as control, high ridge and plastic film mulching as treatment, the suitability of high ridge and plastic film mulching technology in tobacco-growing area in Chenzhou city was investigated, the suitable transplanting period and depth was found. Two factors, transplanting time and transplanting depth, each with three gradients, were used to compare the agronomic characters, economic benefits, chemical composition, coordination and sensory evaluation quality of tobacco leaves in each experimental area at maturity by using the randomized block experiment. The results show that the high ridge and plastic film mulching technology can ensure the normal growth of tobacco plants under normal or dry climate. The agronomic traits, economic benefits, chemical components, coordination and sensory evaluation of tobacco plants with high ridge and plastic film mulching are better than the control tobacco plants. High ridge and plastic film mulching technology is suitable for tobacco-growing area in Chenzhou city. The yield and quality of tobacco would be the best by using D2S3 treatment （transplanting period is March 16, transplanting depth 20 cm）.

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Changes of growth regulators of Camellia sinensis （L.） Kuntze var.niaowangensis Q.H.Chen under low temperature stress in Guizhou

WANG Ying, LI Yan, WANG Shu, XIANG Zhun, ZHAO De-gang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 99-102. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.022

Abstract （483）

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To understand the changes of growth regulators in tea plant [Camellia sinensis （L.） Kuntze var.niaowangensis Q.H.Chen] under the different low-temperature in Guizhou province, one-year-old tea plants of the cultivar were used as the material and the changes of growth regulators content were determined by enzyme-linked immunosorbent assay （ELISA）. Compared to the control, Camellia sinensis （L.） Kuntze var.niaowangensis Q.H.Chen mostly exhibited lower contents in IAA、GA, and ABA content,ABA/GA were higher than control under the 4 ℃and 1 ℃ treatment. While mostly contents of ABA、IAA、GA were significantly higher than control and ABA/GA was no significant change in the 10 ℃ treatment. With the increase of stress time, the content of ABA and IAA increased first and then decreased with the change of time, the content of GA decreased, the ratio of ABA/GA increased, and the content of ABA and GA increased first and then decreased at 10 ℃, the content of IAA decreased. Under low temperature, Camellia sinensis （L.） Kuntze var.niaowangensis Q.H.Chen can improve the growth inhibiting substance such as ABA and reduce the growth promoting substances such as IAA and GA, in order to adjust the endogenous hormonesbalance in plants to cope with low temperatures.

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Analysis and research on flowering period of original ecological flowering cherry community in Gexianshan scenic spot of Xianning city

WEI Hua-bing, LI Lei, LUO Yu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 103-106. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.023

Abstract （394）

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Using the nine years of flowering survey data of wild flowering cherry in Gexianshan scenic spot of Xianning city from 2011 to 2019 and the observation data of the adjacent area meteorology station in the same period,the flowering characteristics and the accumulated temperature forecast indicators of the original ecological flowering cherry community in Gexianshan scenic spot were analysed according to the basic theory of constant accumulated temperature of crop development, using the phase rule of accumulated temperature, double scale statistical recursive generalized discrete coefficient method. The results showed that the average dates of the initial time of flowering and the full-bloom stage of flowering cherry in Gexianshan scenic spot were March 11 to March 12 and March 15 to March 16, respectively, and the average interval of the initial time of flowering and the full-bloom stage was 4~5 days. The florescence index of flowering cherry in scenic spot were since January 11, ≥1.1 ℃ phase accumulated temperature of 316.8 ℃ and 369.7 ℃ respectively by the stage accumulated temperature method. The mean error of this index in predicting flowering was 2~3 days.

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Effects of different disinfection methods and germination matrix on seed germination of 10 species of Rhododendron L.

LIU Guang-chao, TIAN Xiao-ling, LI Xiu-yang, HUANG Cheng-ling

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 107-110. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.024

Abstract （433）

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To explore the effects of different disinfection methods and germination matrix of rhododendron, 10 species of Rhododendron L. were tested, disinfection treatment were as follows, disinfect with a concentration of 0.3% of the potassium acid solution for 10 minutes, disinfect with 1% sodium hypochlorite solution for 10 minutes, disinfect 10 min with 0.1% Hg solution, disinfect with 70% alcohol for 30 seconds +1% sodium hypochlorite for 10 minutes,disinfect with 70% alcohol for 30 seconds+0.1% Hg solution for 10 minutes. In addition, the germination rate, germination potential and germination time of 10 species of Rhododendron L. seeds were determined by filter paper, sponge, gauze and specific culture medium. The results showed that, different disinfection methods had different effects on germination rate and germination potential of different Rhododendron L.. But on the whole, the germination rate and germination potential of 1%NaClO and 70% alcohol +1%NaClO were higher than other disinfection methods. And the delay of germination were also significantly shorter than other disinfection methods. Agar culture medium was suitable for most of the Rhododendron L. seed germination, and the gauze was not suitable for germination matrix of Rhododendron L. seed .

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Evaluation on the growth potential of Ginkgo biloba L. and Lagerstroemia indica L. planted in road green space of Chongqing city

LI Ling-li, ZOU Shi-hui, YU Zhi-yong, WANG Bao-ning, YANG Li-jun, ZHOU Li

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 111-115. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.025

Abstract （366）

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10 plants of Ginkgo biloba L. and Lagerstroemia indica L. were selected from the road green space and nursery of Chongqing city, measured the phenophase, the root index, physiological index and the branches growth indexes, in order to comprehensively evaluate the growth potential of two cultivated varieties, so as to provide the reference for the evaluation of landscape maintenance and landscape seedling selection.

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Preparation and antioxidant evaluation on hardleaf oatchestnut starch/carboxymethyl chitosan blended-membrane

WANG Jie, XIA Cai-fen, ZHOU Li, TIAN Xiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 116-120. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.026

Abstract （384）

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The blended-membrane was prepared with hardleaf oatchestnut starch （HOS） as antioxidant, carboxymethyl chitosan （CMC） and polyethylene glycol （PEG） as casting solutions by high speed shearing mode. The morphology and antioxidant activity of the HOS/CMC/PEG blended-membrane were characterized by scanning electron microscopy and oxidant tests. The results showed the HOS was evenly distributed in the blended-membrane. The scavenging rate of DPPH free radical was 11.92% when the content of HOS was 13% （weight percent）. The scavenging rate of superoxide anion was 15.26% when the content of HOS was 10%（weight percent）. And the scavenging rate of hydroxyl free radical was up to 40.57% when the content of HOS was 7%.

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Effects of different storage conditions on the germination vigor of Haloxylon ammodendron（C.A.Mey.）Bunge seeds

WANG Ze, REN Cai, LIANG Yan, YAN An, MA Hao, LUO Yan-li, SU Ling

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 121-125. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.027

Abstract （458）

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The mature Haloxylon ammodendron（C.A.Mey.）Bunge seeds in Turpan were used as experimental materials, to study the effects of storage time, temperature and seed moisture content on the germination vigor of H.ammodendron（C.A.Mey.）Bunge seeds.The results showed that, under the storage mode of room temperature and refrigeratort for nine months, with the extension of storage time, the seed germination percentage showed a decrease trend, and the rate of decline were 46.50 and 35.50 percentage point. The reduction of germination percentage of storage seeds in refrigerator was lower than room temperature in various periods. Under different storage temperatures for three months, the characteristics of germination percentage, germination potential, germination index and fresh weight of embryo were -10 ℃>-18 ℃>-4 ℃>4 ℃>room temperature. With the storage temperature was decreased, the trend was increased first, then decreased significantly. Under different seed moisture content stored three months, the characteristics of germination percentages, germination index, root length and vigor index of seeds were as follows, with the seed moisture content was decreased, the trend was increased first, then decreased, and the reduction was lowest under the storage conditions of seed moisture content of 5.50%. There were extremely significant negative correlation between storage temperature and vigor index, between storage seed moisture content and germination index,vigor index, respectively. The order of correlation coefficient between seed germination percentages, vigor index and different storage conditions was moisture content>storage temperature>storage time, however,the order of correlation coefficient between seed germination potential,vigor index and different storage conditions was storage temperature> moisture content>storage time.

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Study on quality of Pinellia ternate（Thunb.） Breit of Xixian county from different sampling points

WANG Hai-yan, LI Jin-ping, ZHOU Bin, DONG Zhi-yao, CHEN Qiong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 126-128. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.028

Abstract （445）

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From the characteristics, powder microscopy, TLC, water content, total ash, extract examination and total acid content, the quality differences of Pinellia ternate（Thunb.） Breit of Xixian county from 7 sampling points were compared. Results showed that Pinellia ternate（Thunb.） Breit powder of Xixian county from 7 sampling points have the same microscopic characteristics and the results of TLC, but their characters, water content, total ash content, extracts and total acid content are different. Provenance, breeding method, wild environment and rearing condition have influence on the quality.

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Determination of fatty acids in vegetable oil by gas chromatography

LIU Ying-sha, CUI Li-hui, ZHAO Lu, LI Guo-xiu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 129-133. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.029

Abstract （784）

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The fatty acids of 11 kinds of oil samples and 13 kinds of oil products in the market were determined by gas chromatography, and the fatty acid content and composition of oil samples were compared. Through the addition ratio of oleic acid, linoleic acid and linolenic acid, 13 kinds of oil samples had no significant difference, but for the three indexes of oleic acid, linoleic acid and linolenic acid of 13 all the oil samples were different.

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Determination of four kinds of inorganic anions in different animal skin gelatin and E jiao from different manufacturers

LIU Wen, LI Feng, QIN Jie-ping, FENG Xu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 134-137. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.030

Abstract （362）

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To establish a method to determine the contents of Cl^-,SO₄^2-,NO₃^-,PO₄^3- in animal skin gelatin and E jiao from different manufacturers by IEC. Results showed that Cl^-,SO₄^2-,NO₃^-,PO₄^3- were well separated and showed good linearity at 0.000 922~0.023 1, 0.001 09~0.027 3, 0.000 167~0.004 18, and 0.000 314~0.007 86 mg/mL. The average recoveries were between 98.7%~101.1%. Result of this method was accurate, sensitive, credible and repeatable. It could be applied to the content determination of 4 kinds of inorganic anions in animal skin gelatin and E jiao from different manufacturers.

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Determination of total flavonoids and mangiferin in mango flesh

LIU Chang-kai, YANG Li, HE Lin-fei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 138-140. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.031

Abstract （675）

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A method for the determination of total flavonoids and mangiferin in mango flesh was established. The total flavonoids in mango flesh were determined by uv-spectrophotometer. Mangiferin was determined by high performance liquid chromatography. The results showed that the total flavonoids had a good linear relationship in the concentration range of 17~520 μg/mL （r=0.999 9）, the average recovery was 99.03%, and the RSD was 1.42% （n=6）. Mangiferin showed a good linear relationship in the concentration range of 4.0~80 μg/mL （r=0.999 9）, with an average recovery of 101.59% and RSD of 1.87% （n=6）. The method developed in this paper was simple, accurate and reproducible. It can be used for the determination of total flavonoids and mangiferin in mango flesh.

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Determination of aroma components of Santalum album linn by solid phase microextraction-gas chromatography-mass spectrometry

LI Mei-qin, LU Bin, LI Fang, ABULIZAILI Aili, LIU Xiao, CHEN Guo-tong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 141-144. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.032

Abstract （439）

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The volatile components of sandalwood were enriched by solid phase microextraction and analyzed by gas chromatography-mass spectrometry. The relative content of each component was calculated by area normalization method. The results showed that under normal temperature and drying condition,39 chemical constituents were identified,including olefins （91.15%）, alkanes （3.00%）,alcohols （2.56%）,esters （2.19%）,ketones （0.55%）,aldehydes （0.41%） and heterocycles （0.14%）. This method has the advantages of less sample consumption, easy operation, rapid identification of aroma components and high sensitivity. It could effectively separate and determine volatile substances in sandalwood, realize rapid identification of different sandalwood varieties, and provide effective technical support for further research of Chinese medicinal materials.

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Research on construction safety early warning based on BIM and RFID sensor integration technology

CHEN Yue-hua, SHEN Qian-yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 145-150. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.033

Abstract （450）

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In order to reduce the rate of safety accidents and improve the level of safety management, the integrated technology of BIM and RFID sensors were introduced, and from the perspective of danger source management and control, three sources of danger： people, objects and mechanical equipment were identified, and a safety early warning system for construction sites was constructed. Finally, the tomographic analysis method and fuzzy comprehensive evaluation method were used to evaluate the safety management level before and after using the system. The results showed that this system could significantly improve the safety management level of the construction site and provided a new management method for safety management.

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Optimization of efficiency of shell breaking of Camellia shell breaking machine

HU Yi-fei, WANG Yan-qing, HUANGFU Si-si

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 151-153. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.034

Abstract （454）

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The purpose of this study was to optimize the performance index of camellia shell breaking machine. The parameters affecting the Camellia shell breaking machine were determined by the machine design parameters, namely the roller speed, the track angle, and the cylinder pressure, and a single factor orthogonal test was performed on it.The results showed that the cylinder pressure> stick speed> track angle, and the best working condition of the camellia shell breaking machine was 40° track angle, roller speed 80 r/min, and cylinder pressure 3 bar, Under these conditions, the shell breaking rate was 94.5%, the sub-breaking rate was 1.8%, and the shell-containing rate was 17.1%, it met the working requirements..

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Method of clustering segmentation for tea sprouts based on sharpness function

HUANG Tao, FANG Meng-rui, XIA Hua-kun, ZUO Liang-liang, LYU Jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 154-157. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.035

Abstract （467）

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Aiming at the problem of over-segmentation and under-segmentation in tea sprouts segmentation under natural environment, a new method based on sharpness function evaluation and color clustering was proposed. The image regions with high sharpness function were obtained by combining Tenengrad gradient evaluation and slider analysis, then K-means clustering segmentation under RGB, HSV, Lab, YCbCr color models were finished. Experiments showed that the upper quartile of Tenengrad gradient value as the threshold, the missed selection rate was 25%;Under the HSV color model, the segmentation of tea sprouts was achieved by K-means clustering methods, and the segmentation accuracy in sunny and cloudy were 72.48% and 77.83%. Compared with the direct K-means segmentation method, the false positive rate are reduced 5.19% and 2.03%. The method could segment tea sprouts in natural environment effectively,reduce under-segmentation accuracy and over-segmentation rate, provide theoretical reference for intelligent picking.

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Callus proliferation and bud differentiation of Gynostemma pentaphyllum

CAI Zheng-wang, LIU Jing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 158-160. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.036

Abstract （427）

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With stem of Gynostemma pentaphyllum as explants, MS was used as the basic medium, and different plant growth additives were added to optimize the optimal medium for inducing callus, bud differentiation and rooting. Results showed that the optimal culture medium for callus proliferation was MS+2,4-D 2.0 mg/L +NAA 0.01 mg/L, callus volume increased 2.7 times; The most suitable bud differentiation medium was MS + 6-BA 2.0 mg/L + NAA 0.2 mg/L, the average number of bud 5.7; To take root the optimal medium for MS culture medium added 0.1 mg/L NAA, the rooting rate was 91%.

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Bioinformatics analysis of tobacco polyphenol oxidase

ZHANG Yong-fen, WANG Ping

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 161-163. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.037

Abstract （425）

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In order to carry out bioinformatics analysis of polyphenol oxidase in tobacco, the tools of ProtScale, PhyML and Meme were used to read polyphenol oxidase, and the hydrophobicity, phylogenetic tree and conserved motif were analyzed. The results showed that all members of tobacco polyphenol oxidase were hydrophilic amino acids; the phylogenetic tree of polyphenol oxidase members was divided into three categories, among which tobacco and tomato were clustered, and the second group had the most members; NtPPO1, NtPPO2 and NtPPO3 contained the same conserved motifs, and the positions of NtPPO1 and NtPPO2 were similar, and there were some differences between NtPPO3 and them.

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Study on the evolution and driving factors of ecological environment in Shanxi province

GUO Peng-cheng, ZHANG Xiao-ping

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 164-168. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.038

Abstract （405）

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Taking Shanxi province as the evaluation unit, the evolution process and trend of the ecological environment were analyzed in Shanxi province from 2005 to 2016 on the basis of the main socio-economic data,the index system of driving factors of the ecological environment evolution was constructed based on the structural changes and pollution and treatment. By means of principal component analysis, the driving factors of ecological environment evolution in Shanxi province were discussed. The results showed that during 2005 and 2016, the ecological environment in Shanxi province experienced the process of deterioration first and then improved, and showed the trend of gradual improvement. The first principal component was the main driving factor, which reflected that the economic system played the most important driving role in the evolution of ecological environment in Shanxi province. Industrial structure and environmental governance were the internal and external driving forces of the evolution of regional ecological environment, respectively. Pollutant emission and agricultural production were the secondary driving factors, the concept of socio-economic development and regional eco-environmental quality influenced each other to promote regional sustainable development.

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Investigation and evaluation of mining relic resources in Yaozhihe national mining park,Baokang county,Hubei province

YAN Zhi-wu, TIAN Qi, XIE Yun-hu, ZHOU Ling

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 169-172. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.039

Abstract （359）

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Mining relic is the core landscape of the mine park, was an important natural tourism resources, mining relic survey is of great significance to the mine park. Taking yaozhihe national mining park in baokang, hubei province as an example, this paper classified and evaluated the mining relics of the park. Among them, the discovery of paleo-microbody biogeochemical group and typical sedimentary phosphate deposits in the sinian doushantuo formation had important scientific value. Therefore, the research results should provided technical support for scientific research, protection, rational exploitation and utilization of mining relic resources.

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SWOT quantitative analysis of walnut industry development in Shangluo of Qinba mountain area

GUO Meng, ZHANG Shuai, WANG Yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 173-177. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.040

Abstract （426）

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The development of industry was the fundamental strategy to get rid of poverty. Taking the walnut industry of Shangluo City in Qinba mountain area as the research object, this paper used AHP-SWOT research method to construct strategic quadrilateral, calculated strategic type azimuth, drew industrial development strategic type and intensitied map, and carry out quantitative research on strategic decision-making problems described qualitatively. The conclusion showed that the advantages of walnut industry development in Shangluo were higher than the disadvantages, the development opportunities were greater than the threats, the strategic coefficient ρ was greater than 0.5, the development strategy type was the strength type development strategy, and the plan to seize the external development opportunities and make full use of its own advantages was the key direction of future development. Based on this, this paper put forward some countermeasures and suggestions, such as grasping the policy and market opportunities, doing a good job in the top-level design of industrial development, increasing investment in science and technology and talents, and integrating resources and brand advantages.

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On the mode choice of ecological environment damage relief in China

YU Xing-kang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 178-181. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.041

Abstract （374）

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Based on the consultation system of ecological and environmental compensation system, this paper introduced the main modes of ecological damage relief in China macroscopically, analyzed the shortcomings of different modes, and proposed that the public law thinking should be advocated to construct the mode of ecological and environmental damage relief which was dominated by public law and supplemented by private law.

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Clean development mechanism of Enshi based on the “Two Mountain” Theory——Research on the project of household biogas and carbon sequestration forest

FU Shou-kang, QIN Wu-feng, ZHANG Rong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 182-188. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.042

Abstract （345）

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Under the background of construction of ecological civilization system, poverty was prominent for the realization of ecological resources enrichment of hubei enshi green development, according to the theory of "two mountain" to guide the enshi green development practice, through the door with methane and carbon sink forest project research, discussed the status and existing problems of the clean development mechanism would be, was put forward through the energy production and consumption structure optimization.Focusing on the construction of household biogas and carbon sequestration projects, we would strengthen the capacity building of the clean development mechanism, optimized the ecological compensation mechanism, and introduce more resources into the green poverty reduction in a market-oriented way.

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Study on quality cost accounting of agricultural enterprises based on Activity-Based Costing：Taking JH company as an example

DONG Mei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 189-193. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.043

Abstract （396）

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In the management of agricultural enterprises, quality cost accounting plays a key role in quality management decision. Taking JH company as an example, based on Activity-Based Costing, the application of Activity-Based Costing in the quality cost accounting of agricultural enterprises was analyzed by constructing a mathematical calculation model of Activity-Based Costing. Comparing the results of Activity-Based Costing and traditional costing,the results showed that Activity-Based Costing could calculate the corresponding cost of each product and provide information basis for enterprise management. Therefore, it is necessary to strengthen the basic work of quality cost accounting of agricultural enterprises, establish quality cost assessment system, and realize quality cost information sharing and communication, so as to make the Activity-Based Costing play a role in the quality cost accounting management of agricultural enterprises.

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Analysis of driving factors of agricultural water in Yangtze River economic belt based on LMDI

SHENG Qian, XIA Yu-xin, SHEN Xiao-mei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 194-198. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.044

Abstract （446）

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Optimal allocation of water resources has become the core issue of green agricultural development in the Yangtze River Economic Belt. It is the key to clarify the internal driving factors and effects of agricultural water change. Based on the panel data of 11 provinces and cities in the Yangtze River Economic Belt from 2002 to 2017, the influencing factors of agricultural water was divided into technical level, industrial structure, production level and population size by using the logarithmic mean divisia index method. Furthermore, the effects of different factors on agricultural water were analyzed. The results showed that, as a whole, the output effect and population effect promoted the growth of agricultural water, in which the output effect was dominant, and the technology effect and the structure effect restrained the agricultural water. From a regional perspective, Shanghai city, Anhui province, Jiangxi province, Hubei province, Chongqing city, Sichuan province and Guizhou province played an overall role in promoting agricultural water. Jiangsu province, Zhejiang province, Hunan province and Yunnan province had a restraining effect on agricultural water.

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Risk assessment of greening existing buildings in China based on GA-BP neural network

CHEN Yue-hua, ZHANG Rui-qi, LI Xiao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 199-205. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.045

Abstract （368）

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There are many obstacles in the process of green renovation of existing buildings in China, which not only slows down the development of green renovation, but also increases the risk of renovation projects. In this study, the risk evaluation index system for green renovation of existing buildings is established for the retrofitting party. According to the different degree of impact of each risk factor, the weight of each index is given by the AHP method. The Matlab software is used to train the established GA-BP neural network, which is applied to the post-risk evaluation of the green transformation of the building and the risk assessment of the proposed green transformation project. The results show that for the reformer, risk management in the decision-making and design stage should be increased.

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Government subsidies, R&D expenditure and performance of agricultural listed companies：An empirical study based on 44 agricultural listed companies in Shanghai and Shenzhen A stock market

QIU Xiao-xue, ZHANG Ying

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 206-209. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.046

Abstract （433）

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Based on market failure and technological innovation theory, the 44 agricultural listed companies in the Shanghai-Shenzhen A-share market from 2016 to 2018 were used as the research samples to study the relationship between government subsidies, R & D expenditure and the performance of listed agricultural companies. It also considers the intermediary effect of R&D expenditure on government subsidies and corporate performance. The results show that government subsidies have a significant promotion effect on listed agricultural enterprises‘ R&D expenditure and corporate performance; listed agricultural enterprises’ R&D expenditure is significantly positively correlated with their corporate performance, and there is a significant difference between the current government subsidies and agricultural enterprise performance Mediation effect.

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Empirical study on the impact of farmers' income structure on non-grain farmland based on PVAR

LI Ya-ya, ZHAO Xiao-feng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 210-213. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.047

Abstract （371）

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The development of urbanization, industrialization and the liberalization of the separation of powers of contracted land have accelerated the non-grain phenomenon of farmland. Using the PVAR model, the dynamic relationship between farmers' income and non-grain farmland was analyzed, and the impact of farmers' income structure on non-grain farmland was discussed. The results show that from 2002 to 2017, the change trend of non-grain area and non-grain rate of farmland is basically the same, showing different characteristics at different stages under the influence of economic development and national policies. Non-grain farmland is mainly affected by farmers' wage income. Household operating income, property income and transfer income have a significant positive effect on non-grain farmland, but the long-term contribution rate is small.

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Challenges and countermeasures for the conservation and sustainable utilization of biodiversity for food and agriculture

TIAN Xue

HUBEI AGRICULTURAL SCIENCES 2020, 59 (8): 214-217. DOI: 10.14088/j.cnki.issn0439-8114.2020.08.048

Abstract （327）

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The importance and role of food and agricultural biodiversity were discussed, the challenges faced by the world's food and agricultural biodiversity were analyzed, and the countermeasures for protecting food and agricultural biodiversity in China were described. The results show that many key components of food and agricultural biodiversity are gradually shrinking, and the lack of data often limits the planning and sequencing of effective remedial measures. Some countermeasures for the protection and sustainable use of food and agricultural biodiversity in China have been proposed.

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Advances in the application of acidifiers in aquaculture

MA Qiu-yue, SUN Ru-jiang, XIAO Fa-yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 5-9. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.001

Abstract （478）

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Acidifier is a new type of green and environmentally friendly feed additive that is considered an effective alternative to antibiotics. Currently, the application of acidifiers in livestock and poultry farming has become increasingly mature, and its use in aquaculture has become more and more extensive in recent years. The research progress on the classification, mechanism and application of acidulants is reviewed, with a view to providing a certain reference for the substitution of acidulants for antibiotics and promoting the sustainable development of aquaculture.

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A summarize of the research on tourism niche in China

ZHAN Xue, ZHANG Jun-xia

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 10-14. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.002

Abstract （483）

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The tourism niche comprehensively reflects the relative position and role of tourism in the dimensions of resources, markets, services and environment. The study of tourism niche is of great significance in improving tourism competitiveness, promoting coordinated development of regional tourism, and optimizing the layout of tourism space. From 2001 to 2018, the research trends of tourism niche in China have three stages,the research focuses on the evaluation of tourism niche, the tourism competition and cooperation research and the tourism spatial structure three aspects. The research method is mainly based on quantitative research, and the niche measurement models are most widely used, combined with analytic hierarchy process and cluster analysis method. However, there are still several shortcomings in the research, first, the interdisciplinary research is not deep enough, and it stays on the theoretical basis; second, the lack of innovation in tourism niche optimization strategy is limited to niche separation, niche expansion and compression, niche generalization and niche specialization strategy; third, the scope of the research object is macroscopic, and the relevant research on county and township is dificient.

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Analysis on crop productivity, stability,adaptability of maize hybrid Huayu 11 and its cultivation technique for high yield

YU Chang-ping, ZHOU Hua-ping, YE Qing-song, XIAO Neng-wu, XIAO Li-li, LIU Yong-zhong, QIN Guang-ming

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 15-19. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.003

Abstract （823）

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According to the data of Hubei provincial regional experiment and productive administration of maize in southwest China, the performance of productivity, stability and adaptability of Huayu 11 was analysed by testing the average yield,variation coefficient, high yield effect value, high stability coefficient, standard deviation method. The results show that Huayu 11 possesses higher yield, more strong stability, wider adaptability, and suitable for planting in southwest China and other corresponding ecological areas.To gain cultivation technique of high yield of Huayu11, the factor of fertilizer amount（F） and density（M） was designed on five levels in this experiment. The scheme for getting yield of 15 993 kg/hm² was that plant density of 60 000 plants/hm²,750.0 kg/hm² of fertilizer as base fertilizer and 450 kg/hm² of urea for topdressing（seed fertilizer:panicle fertilizer=1:2）.

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Study on meteorological grade prediction of Chilo suppressalis （Walker） in Zigong city

LUO Wei, DUAN Xiu-rong, ZHONG Li, WANG Ru-lin, LI Qiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 20-23. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.004

Abstract （431）

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The effect of meteorological conditions on occurrence of Chilo suppressalis （Walker） of rice（Oryza sativa L.） in Zigong city, Sichuan province were studied by using historical and meteorological data of the same period from 2000 to 2018. Meteorological factors with significant correlation and clear biological significance were selected through the analysis of the quantity of inducible moth of Chilo suppressalis （Walker） under the lamp and meteorological condition, and the multiple regression analysis was used to establish the meteorological forecast model of Chilo suppressalis （Walker） in Zigong city. The results showed that the average temperature in winter in Zigong city had the most significant influence on the meteorological level of Chilo suppressalis （Walker）, followed by the temperature, precipitation and humidity in each period of the following year, and the higher winter temperature was the most important meteorological factor that caused the meteorological grade of Chilo suppressalis （Walker） to be 3 ~ 4 for many years. The fitting accuracy rate of meteorological grade of historical regression test reached 72%, and the accuracy rate of meteorological grade of 2018 test report reached 100%, indicated that the model has a good prediction effect and can provide scientific meteorological decision-making basis for the control of Chilo suppressalis （Walker） in Zigong city.

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Forecast of meteorological grades of winter wheat snow mold in Changji

JIAN Yong-mei, ZHANG Jian-yun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 24-27. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.005

Abstract （379）

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Snow mold was the main diseases of winter wheat in Changji Hui Autonomous Prefecture, Xinjiang Uygur Autonomous Region, they occur every year to varying degrees, and they form an onset cycle with changes in weather conditions. By collecting disease data from 1996 to 2018 and field surveys, the correlation between winter wheat snow mold incidence and 19 meteorological factors such as maximum snow depth in winter, ≥20 cm snow days, ≥30 cm snow days, precipitation in mid-March, average temperature in late-March were analyzed. The strong correlation meteorological factors was screened out, and a meteorological grade forecasting model for winter wheat snow mold rot was established. The application test has a good forecasting effect.

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Effects of different application methods of compound amino acid fertilizer on eggplant growth

YAN Ting-shuai, FAN Zhao-bo, LI Xin-zhu, SHI Li-bo

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 28-31. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.006

Abstract （450）

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The effects of different application methods （spraying, watering, spraying and watering） of compound amino acid fertilizer on net photosynthetic rate, agronomic index and element content （total N, total P, total K, total Mg, total S） on eggplant（Solanum melongena L.） were studied. The results showed that there was no positive effect on net photo synthetic rate of eggplant by applying compound amino acid fertilizer; The fresh weight on the grond and weight of single fruit increased by application of compound amino acid fertilizer, and there was no significant difference among the ways of applying compound amino acid fertilizer; The different application methods of the compound amino acid fertilizers affected the content and distribution of total N and total P, total K and total S. In summary, spraying and watering compound amino acid fertilizer has the best promotion effect to the growth of eggplants.

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The influence of waterlogging stress on yield and quality at different pod stage of peanut

MA Qing-rong, ZOU Chun-hui, HU Cheng-da, CHENG Lin, LI Tong-xiao, QIN Fu-sheng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 32-36. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.007

Abstract （449）

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Under the background of supply side reform of agriculture, the influence of stress of waterlogging on yield and quality at pod period of peanut could offer important scientific evidence for agricultural disaster prevention and reduction. Based on field experiments, the whole pod stage of summer peanut was divided into early, middle and late periods, and in each period, wet stress lasted 0 （control group）, 3, 5, 7 and 9 days respectively were set for studying the final impact of wet hazard on peanut. The results showed that, wet stress happened earlier and the days lasted longer, the greater was the impact on the production. The overall trend of influence showed the impact in early stage was bigger than that of middle stage and late stage, and the influence lasted 9 days was bigger than that of 7, 5 and 3 days. In early stage, 9 days lasted waterlogging reduced the pod yield, fruit weight and kernel weight by 21.8%, 16.2% and 24.6% respectively, and which were 15.7%, 8.6%, 14.2% in the middle stage. Stress of 3 and 5 days occurred in early and middle stage were positive, and the average yield increased 8%, however, the effect was negative when the stress lasted more than 7 days. Wet stained stress also influence the quality of seed kernel, and the influence degree was bigger in middle stage than late and early stage. Moisture, protein and oleic acid content increased, meanwhile, amounts of fat, linoleic acid and palmitic acid decreased, so the quality decreased. Moisture and fat content increased with the increase of the waterlogging days, while the rest of the quality index was not obvious correlated to hazard days.

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Analysis on the characteristics and countermeasures of water resources in Baoji city in the 21st century

ZHANG Xin-di, REN Yuan-xin, ZHANG Hai-ning, ZHOU Qi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 37-40. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.008

Abstract （375）

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Through the analysis of time characteristics the data of current water resources in Baoji city, Shaanxi province, from 2000 to 2018, it finds that the amount of surface water resources occupies a dominant position in the total amount of water resources. The total water consumption in Baoji city is increasing, among which agricultural water consumption accounts for the largest proportion of the total water consumption. At the same time, it is faced with three major problems： uneven distribution of water resources, increasing water pressure and the need to improve the discharge of domestic sewage. According to the existing problems, three ideas of specific countermeasures of developing “passing water”, protecting “own water” and skillfully using “extreme water” were put forward to solve the problem.

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Characteristics and source analysis of carbonaceous composition, water-soluble ion concentration in heavy pollution period in Xuzhou city

JIA Hong, LIU Zi-he, XI Lin, ZHANG Fang-fang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 41-48. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.009

Abstract （407）

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Based on the Model 2001A thermal/photocarbon analyzer developed by the US Desert, the carbonaceous components （organic carbon （OC） and elemental carbon （EC）） and water soluble ions （NO₃^-, SO₄^2-, F^-, Cl^-, NO₂^-, NH₄⁺, K⁺, Ca²⁺, Mg²⁺, Na⁺） in PM2.5 during heavy pollution in 2016 winter in Xuzhou city were monitored by day and night sampling, and the secondary organic aerosol （SOC） content was optimized by the optimized MRS algorithm. The results showed that the average mass concentration of PM2.5 in Xuzhou city reached （129.7±37.0） μg/m³ during the sampling period. Through the OC/EC ratio analysis, the carbonaceous aerosol in Xuzhou city during the sampling period was mainly affected by the exhaust emissions of gasoline vehicles and diesel vehicles. The average mass concentration of SOC was 3.4 μg/m³, and the contribution to OC reached 44.3%, and the degree of secondary pollution at night was greater than that during the day. The average mass concentration of water-soluble ions in the heavy pollution period reached （126.0±24.0） μg/m³, and the three main water-soluble ions of NO₃^-, SO₄^2-, and NH₄⁺ existed in the form of NH₄NO₃ and （NH₄）₂ SO₄. Through the analysis of NO₃^- and SO₄^2- ratios, the fixed source of coal-burning contributed a lot to water-soluble ions. Using PMF model to analyze the mass concentration of atmospheric PM2.5 in heavy pollution period in Xuzhou city, there were mainly six, including traffic source （48.7%）, secondary inorganic aerosol pollution source （24.3%）, sea salt and coal combustion source （14.9%）, secondary chemical pollution sources （12.1%）, biomass combustion sources （0.9%）, and road dust sources （0.1%）. In general, the source of atmospheric PM2.5 in Xuzhou city is more multi-sourced, and the traffic source and secondary inorganic aerosol pollution source occupy a dominant position.

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Analysis and forecast about the indexs of strong cold air from east road in northern Jiangsu province

ZHANG Fang-fang, ZHAO Jie, SUN Lei, ZHAO Yu, WU Yan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 49-53. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.010

Abstract （363）

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Meteorological statistics from 2001 to 2010 of northern Jiangsu province was used to divided into four types in accordance with the standards of the cold air from east road, then forecast indexes were obtained according to the characteristics of each model.The relationship between the 48 hours cooling amplitude of the 850 hPa and 9250 hPa with the ground of eligible cases within 10 years was analyzed, then the data from 2011 to 2012 was chosen to forecast according to the standards of indexes. The results showed that the prediction accuracy of east road cold air was over 90%, and the type of two troughs with one ridge was the most, followed by the horizontal trough turned vertical type and blocking high type, the low trough eastward type was not appear. The cooling amplitude of upper temperature has relatively good referential meaning for the ground temperature drop.

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Spatiotemporal characteristics of hail disasters in Hebei from 1644 to 1948

LIU Hao, WEI Jun, CHEN Sha, XIE Wen-juan, ZHAO Liang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 54-59. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.011

Abstract （416）

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The records of hail disasters in Hebei during the Qing and Republican periods（1644—1948） were analyzed, and the spatial and temporal distribution and causes of hail disasters were studied. The results show that hail disasters occurred frequently in Hebei during this period, and the annual frequency showed a trend of turbulence. The hail disasters were prone to occur in the early Qing Dynasty and the late Qing Dynasty to the middle of the Republic of China. Hail disasters mostly occur in summer and autumn, and the probability of mild hail disasters is the highest; and the probability of moderate and severe hail disasters is similar. Mountain areas and plains are more prone to hail disasters, of which the Taihang Mountains, Yanshan Mountains, and the Central and South Plains of Hebei are the areas most prone to hail disasters. Hail disasters have been recorded in most areas of Hebei, which are the provinces that have suffered severe hail disasters.

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Analysis of precipitation characteristics in the west of Hexi Corridor from May to September in recent 41 years

LI Jian, CHEN Li-na, TAO Sheng-cai, ZHANG Wen-jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 60-64. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.012

Abstract （439）

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Using the daily precipitation data（20：00-20：00） from May to September in 1976-2016 of 7 national automatic weather stations in Jiuquan, located in the west of the Hexi Corridor, using statistical methods, the spatial-temporal distribution characteristics and trends of rainy days, precipitation, and extreme precipitation events in Jiuquan were studied. The results show that Jiuquan has the most mild rainy days, accounting for 82.1% of the total rainy days. Nearly 1/3 of the rainfall in May-September is provided by mild rain, and 2/3 are provided by precipitation above moderate rain. The spatial distribution characteristics of rainy days （quantities） at all levels are： the most is Subei, the least is Dunhuang. Meanwhile, the absolute intensity of Jiuquan extreme precipitation events in the past 41 years is relatively small and the relative intensity is large. There is no significant linear trend in the frequency of heavy rainfall events, and it has remained stable at about 0.1 times per year. However, its inter-annual and inter-decadal fluctuations are very obvious. The time distribution of no rainstorm events is relatively scattered, and it occurs in every age. In addition, there were no extreme precipitation events in August-September 2011-2016.

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Effect of El Niño on may precipitation of Tibet

Luosangwangmu, Daqiong, Pubuzhuoma

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 65-69. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.013

Abstract （341）

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Based on the data of NOAA sea surface temperature anomaly field, NCEP/DOE wind field, atmospheric perceptible water field data and PREC/L precipitation data from 1979 to 2017, the effects of El Niño on May precipitation of Tibet were analyzed. The results shows that during the year of El Niño, there is an anti-cyclone circulation deviation at 700 hPa in the Indo-China peninsula, which form subsidences, and there is less perceptible water in the atmosphere and less rainfall in Tibet. In the year of La Nina, a cyclone in the Indo-China peninsula occurs at 700 hPa, resulting in updrafts. The amount of perceptible water in the atmosphere is high, and precipitation in Tibet is high.

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Analysis of NDVI changes and driving factors in Taihang mountain area from 2000 to 2015

LI Xin-yuan, WANG Li, JING Hai-tao, FAN Chen-yu, YU Xun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 70-76. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.014

Abstract （468）

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This research was based on MODIS NDVI data from 2000 to 2015, combined with meteorological, land use and other relevant data of the same period. According to the NDVI change data and land use type change data obtained by relevant calculations, the NDVI change data were reversely studied to find the driving factors that affect NDVI change. The results showed that, from 2000 to 2015, the overall vegetation coverage in the study area had been improved, with an average growth rate of 0.007 5/a. The driving factor for the increase of NDVI was the promotion effect of meteorological factors on vegetation growth. Human activities directly led to the increase of NDVI within the cultivated land by making the cultivated land crops grow more vigorous. Human activities played a role in promoting vegetation in natural areas far from cities, such as Taihang mountain afforestation project, farmland conversion project, rural population transfer to cities and other ecological protection measures, which had a positive impact on the change of NDVI in grassland, woodland and water area. The driving factor of NDVI reduction area was the decrease of cultivated land and grassland area around the city and the increase of urban area in Taihang mountain area. Human activities weaken the vegetation growth in the city and its surrounding areas.

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Physiological characteristics and adaptability of four medicinal plants in karst environment

HAO Jun-xiao, JIN Wen-ge, LI Liang-bo, HUANG Rong-shao, TANG Hui, GAN Feng-qiong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 77-82. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.015

Abstract （582）

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Four medicinal plant species Semiliquidambar cathayensis, Callicarpa nudiflora, Stephania kwangsiensis and Sophora tonkinensis were collected from Napo county, Guangxi province. The diurnal variation of photosynthesis was measured by Li-6400 photosynthetic system, and combined with the determination of chlorophyll content, carbonic anhydrase activity of leaves and stable carbon isotope composition, the adaptability of four plants in Karst habitat of Napo county, Guangxi was analyzed. The results showed that S. Cathayensis and C. nudiflora had better light utilization ability, and their daily average LUE value was S. cathayensis（1.77%） > C. nudiflora（1.65%）. And the optical compensation points of S. cathayensis and C. nudiflora were low. The chlorophyll content and carbonic anhydrase activity of S. kwangsiensis leaves were （3.13±0.20） mg/g and （8 290.00±41.50） WAU/gFW. The activity of carbonic anhydrase in leaves of S. kwangsiensis was higher, which indicated that it had better carbon fixation ability. From July to December, the plant height of S. tonkinensis and C. nudiflora increased by 79 and 36 cm respectively, and the plant grew vigorously. The δ¹³C size of plant leaves is S. cathayensis （-26.684±0.781）‰ > S. kwangsiensis （-27.407±0.078）‰, which was higher than the average value of C₃ plants in karst area. It showed that S. cathayensis and S. kwangsiensis had strong drought resistance ability. The comprehensive analysis showed that the four plants had different adaptation mechanisms to the arid environment in the southwest of Guangxi karst area, showing good adaptability.

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Effect of interplanting soybean with Zanthoxylum bungeanum on physiological characteristics of Z. bungeanum in karst areas of Guangxi

JIN Wen-ge, SHEN Kang-long, HAO Jun-xiao, GAN Feng-qiong, HUANG Rong-shao, LI Liang-bo

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 83-85. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.016

Abstract （481）

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The karst terrain in Guangxi is widespread, and the problem of rocky desertification has seriously affected the local economic development. The development of a reasonable compound planting model in karst areas can bring significant economic benefits and also have a positive effect on the management of rocky desertification. Soybean is a common cash crop in the karst area of Guangxi, and the introduction and promotion of Zanthoxylum bungeanum has also become one of the poverty alleviation industries in the karst area. In the early growth stage of Z. bungeanum, three soybean interplanting densities were set between rows, and their effects on the growth characteristics, leaf water content, and photosynthetic capacity of Z. bungeanum were analyzed. The results showed that the treatment with high intercropping density （45 soybeans/㎡） had higher water content in the leaves of Z. bungeanum and better plant growth; interplanting Z. bungeanum can not only obtain short-term benefits of soybeans, but also enhance the photosynthesis of Z. bungeanum and promote the growth of Z. bungeanum.

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Effects of waterlogging stress on growth and physiology characters of ornamental sunflower seedlings

LUO Jie, YUAN Long-yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 86-90. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.017

Abstract （488）

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Taking ornamental sunflower（Helianthus annuus L.） seedlings as materials, the effects of waterlogging stress on the morphology, physiology and root anatomy of ornamental sunflower seedlings were studied. The results showed that the plant height of ornamental sunflowers were significantly lower than the control （P﹤0.05）, and the roots were significantly less than that of control when they were waterlogged for 8 days. As for the concentrations of malondialdehyde（MDA） in ornamental sunflowers as measured at 0 d to 8 d of waterlogging, there was no significant difference compared with controls. The activity and proline content of superoxide dismutase（SOD） in ornamental sunflowers were significantly higher than that in the control group when the sunflowers were waterlogged for 2 days. The peroxidase（POD） activity, soluble sugar and soluble protein content in ornamental sunflower were significantly higher than those in the control group when waterlogged for 8 days. This indicates that the growth of ornamental sunflowers is inhibited by waterlogging stress, but it has a certain tolerance to waterlogging stress. On the one hand, it can resist waterlogging stress by increasing antioxidant enzyme activity and osmotic regulator content; on the other hand, it can adapt to waterlogging stress by forming aerenchyma in roots.

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Effects of shading on the growth and quality of strawberry

YUE Gao-feng, WANG Li-ping, HAN Zhi-qiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 91-94. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.018

Abstract （568）

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The growth of strawberry （Fragaria ananassa Duchesne） in spring and summer was studied under different shading treatments. The results showed that different shading treatments had significant effects on the growth and development, flowering quality, fruit yield and fruit quality of mature strawberry in spring and summer. Under the condition of 20% light shade, the growth and development status, flowering period, fruit yield and quality of strawberry were the best; Under 40% moderate shade, the content of chlorophyll a and b in strawberry leaves was the highest; Under the condition of more than 60% shade, the growth indexes of strawberry decreased significantly.

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Effects of transfer of fructan synthase gene of Psathyrostachys huashanensis on physiological indexes of tobacco

CHEN Zhen-zhen, ZHOU Guo-qin, CHEN Xin-hong, XIE Xu-dong, CHEN Jin-ping

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 95-98. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.019

Abstract （390）

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HS-6-SFT transgenic tobacco and non-transgenic tobacco W38 were used as materials under low temperature stress at 4 ℃ for 8 h. The differences of soluble protein, superoxide dismutase（SOD）, catalase CAT, peroxidase（POD） between transgenic tobacco and non-transgenic tobacco were analyzed. The results showed that the soluble protein content, SOD activity, CAT activity and POD activity in tobacco leaves increased, and the increase at 4 ℃ was larger than that at room temperature. There was a very significant difference between the transgenic materials and the control（P<0.01）. The stress resistance of transgenic tobacco with HS-6-SFT gene is enhanced.

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Evaluation and selection of appropriate tobacco varieties for Badong, Hubei

FENG Fan-wen, WANG Yu-kui, CHENG Jun-qi, XIE Da-zhi, HUANG Jing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 99-101. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.020

Abstract （442）

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In order to screen out high quality and high yield varieties of flue-cured tobacco suitable for planting in tobacco area of Badong county, three new strains were screened in the field. The results showed that, compared with Yunyan 87, the agronomic and economic traits of three new strains were slightly worse. HB202 performed relatively well; The internal chemical components contents of HB204, HB094 and Yunyan 87 were relatively harmonious. The sensory quality of different varieties of tobacco leaves were as follows, smoking aroma quality was medium, volume of aroma was still adequate, remaining taste was comfortable and stimulating, Yunyan 87 was better than three tested new strains.

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Screening of pineapple varieties suitable for intercropping in macadamia

WEI Qiao-yun, QIU Wen-wu, WANG Wen-lin, WEI Chi-zhang, TAN Qiu-jin, ZHOU Jing, XU Jian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 102-104. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.021

Abstract （424）

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In order to screen pineapple[Ananas comosus （Linn.） Merr.] varieties suitable for interplanting between Australian macadamia（Macadamia ternifolia F. Muell.） orchards, four pineapple varieties including Tainong No. 16, Tai Nong No. 17, Golden Pineapple and Tainong No. 4 were selected by randomized block design. The excellent pineapple varieties were tested in a trial, and the agronomic traits, fruit traits and quality of the plants were investigated, and correlation analysis was carried out. The results showed that the planting height, leaf width, average fruit weight, soluble solid content and soluble sugar content of pineapple in Tainong No.16 pineapple were the highest, the pulp fiber was less, the texture was smooth and the flavor was sweet. According to the comprehensive evaluation, Tainong No. 16 is the most suitable pineapple variety for macadamia interplanting.

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Evaluation of 72 wild woody ornamental plants in Xichuan

SUN Li-qang, LI Yu, GUO Meng-di, TIAN Yu-hui, WANG Peng-fei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 105-110. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.022

Abstract （472）

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Based on field surveys and literature references in Xichuan area of Henan Province, 12 specific indicators were selected from the three aspects of ornamental characteristics, biological characteristics and resource potential of wild plants. AHP was used to establish an evaluation system for wild woody ornamental plants in Xichuan. Using yaahp10.3 software to construct the matrix and calculate the weight of each specific index in the evaluation system, a comprehensive evaluation of the 72 wild woody ornamental plants selected was carried out, and the priority development and utilization levels were divided according to the comprehensive score calculated by the software.

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Resources investigation and analysis of structural feature of street trees in urban area of Zhengzhou

SHI Xi-bing, JIAO Xue-hui, ZHOU Xiao-juan, SUN Yi-ning, LIU Jie, MIAO Zhao-feng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 111-116. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.023

Abstract （487）

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Through stratified random sampling, the structure features, configuration mode and health status of street trees in eight urbans of Zhengzhou city were investigated. The results showed that there were 39 species of street trees, belonging to 22 families and 30 genera, the ratio between evergreen and deciduous trees was 1:6.8. The high frequency of street trees were Platanus hispanica（48.46%）, Sophora japonica（10.38%）, Koelreuteria integrifoliola（8.85%）, Ligustrum incidum（8.46%） and Fraxinus chinensis（6.54%）, and the diversity index was 2.225. Single carriageway with two green belts pattern occupied the main forms. The ratio of single-layer model was 66.91%. The proportion of street trees growing above average was 83.48%. According to the current situation of Zhengzhou, the existing problems can be improved by increasing the number of tree species, enriching the allocation mode and strengthening management and maintenance.

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Viewing the landscape features of garden railings from the perspective of Song painting

LI Jiao, DU Shi-bo, YANG Fang-rong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 117-121. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.024

Abstract （401）

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In ancient times, garden railings not only played a role in safety protection, but also one of the important methods of garden landscape formation, and they were also sentiment images favored by scholars and scholars. Although Song Dynasty gardens are now rare, Song paintings provide precious picture materials for Song Dynasty garden research. The railings in Song paintings are rich in imagery, diverse in type, and elaborately drawn. They can intuitively reflect the construction level and style of garden railings in Song Dynasty, and have important research value. By referring to related books and Song paintings, the origin, classification, function and environmental landscape effect of railings were analyzed, which provides a new perspective for the discussion of Song Dynasty garden landscape and enriches the basic data research of Song gardens. At the same time, the material and form changes of the Song Dynasty and modern railings were compared to provide a reference for the design of garden railings.

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The formation mechanism and empirical test of the folk quality reputation of animal products in China

YOU Xi-huo

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 122-126. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.025

Abstract （384）

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The influencing factors that affect the quality reputation of the animal produces were analyzed, and a model of formation mechanism was constructed. Using the date from questionnaire to test, a brief conclusion was got and suggestions to improve the quality reputation of animal produces were given.

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Status quo and development countermeasures of silage corn in Xiangyang city

FENG Peng, HU Fei, LI Xin-yan, MA Xian-bin, GUO Li, LI You-ming

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 127-129. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.026

Abstract （436）

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The production status quo of silage corn（Zea mays L.） in Xiangyang city, the benefits of planting silage corn and the problems in the implementation process were analyzed. Suggestions to determining the number of cultivation by the number of breeding, promoting collaboration between the department of agriculture and animal husbandry, strengthening government guidance, and industrial development seminars and technical training were put forward, aiming to promoting the development of animal husbandry and adjustment of planting structure in Xiangyang city.

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Effects of Lepidium latifolium L. extracts on rats gastrointestinal motility, SP and Ghrelin

ZHAO Yi-hua, ZHONG Jiang-bin, LI Wei-lin, QI De-sheng, SUN Shi-hao, ZOU Deng-lang, DU Yu-rong, MA Jian-bin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 130-132. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.027

Abstract （304）

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To study the effects of the Lepidiumlatifolium L. extracts on rats with gastrointestinal motility disorder and explore its mechanism, 24 SPF SD rats weighing 180~200 g were randomly divided into blank group, model group and test drug group. Each group had 8 rats and the ratio of male to female is 1:1. Each group was given the corresponding drugs by intragastric administration. Each rat in the test drug group was intragastrically administered with 2 mL solution of the extract from Lepidiumlatifolium L. （0.36 g·mL^-1）. Each rat in the blank group and the model group was intragastrically administrated with equal volume of distilled water. After intragastric administration once a day for 6 days, the rats were fasted for 24 h but free of water. on the 7th day, 1 h after the normal administration, the model group and the test group were intraperitoneally injected with atropine sulfate of 2 mg·kg^-1, and the blank group was intraperitoneally injected with the same volume of normal saline. After 20 min, all rats were intragastrically administered with 0.4 ml of 2% blue dextran-2000 solution. After 30 min, rats were anesthetized with 4 mL·kg^-1 of 10% chloral hydrate. The detect index included gastric emptying rate, small intestinal propulsion rate, the substance P （SP） and ghrelin concentrations in gastric and duodenal homogenate by ELISA. The results showed that the gastric emptying rate and small intestinal propulsion rate in the model group were extremely significant lower than those in blank group（P<0.01） while the gastric and duodenal SP concentrations and the gastric ghrelin concentration presented no significant difference （P>0.05）, and the concentration of duodenal ghrelin was significantly higher （P< 0.01）. Compared with the model group, gastric emptying rate and small intestinal propulsion rate in the test drug group were also significantly increased （P<0.01）, and the concentration of SP in gastric was significantly increased （P< 0.05）; the concentration of ghrelin in gastric and the concentration of SP, ghrelin in duodenal were significantly elevated （P< 0.01）. It indicated that the extracts from Lepidiumlatifolium L. might improve the gastrointestinal motility of rats with gastrointestinal motility disorder induced by atropine sulfate by increasing the concentration of SP and ghrelin in the stomach and duodenum.

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Antitumor effect of EPF monoclonal antibody combined with cisplatine on animal bladder cancer in livestock：Based on a bladder tumor model in nude mice

ZHONG Yong-yi, ZHOU Quan, SU Li-jie, LIAN Yu-yin, WANG Jia-ji, CHEN Xiao-yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 133-136. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.028

Abstract （366）

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To investigate the antitumor effects of early-pregnancy-factor monoclonal antibody （EPF-McAb） combined with cisplatin（DDP） on human bladder cancer EJ cell in nude mice, and to explore its possible mechanism, a melanin xenograft model of nude mice was established. When the diameter of the transplanted tumor grew to 5mm, the mice were randomly divided into 4 groups, a blank group with no treatment, a control group treated with cisplatin, and two treatment group treated with low dosage EPF-McAb combination and high dosage EPF-McAb combination, respectively. The volume of transplanted tumor was measured every 2 days after the administration tp plot the tumor growth curve. On the 10th day after the last administration, the tumors were taken and weighed to calculate the tumor inhibition rate. The pathology changes of carcinoma were observed after HE staining by light microscope. The expression of EPF protein was detected by Western blot. The results showed that the growth rate of the transplanted tumors in the two treat groups were significantly slower than that in the blank group and the control group （P<0.05）. The tumor inhibition rate of the transplanted tumors in the low-dose EPF-McAb group and high-dose EPF-McAb was 35.78% and 53.04%, respectively, which were both significantly higher than that in the control group （with tumor inhibition rate of 22.36%）（P<0.05）. And the expression of EPF protein in treatment groups was also decreased. It indicated that EPF-McAb combined with DDP can effectively inhibit the proliferation of bladder cancer EJ cells in nude mice. The mechanism may be related to down-regulation of EPF protein expression.

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Therapeutic effect of Shaohuang granules on psoriasis mice

CHENG Guo-qiang, DONG Han, WANG Yong-jin, XIA Jie-ying, HONG Yang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 137-140. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.029

Abstract （343）

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To evaluate the effects of Shaohuang granules on psoriasis mice, a imiquimod-induced psoriasis model was constructed and used to observe the influence of Shaohuang granules on the pathological changes, epidermal thickness, the expression of PCNA in psoriatic lesions. The effects of Shaohuang granules on the mitosis of vaginal epithelial cells in estrogen period and the granular layer of mice tail epidermis were also observed. The results showed that Shaohuang granules could obviously decreased the skin thickness of psoriasis mice （P<0.05）. 10 g/kg and 5 g/kg dose of Shaohuang granules could improve the mice skin lesions and reduce the PCNA expressions significantly. 10 g/kg dose of Shaohuang granules could obviously inhibit the mitosis of mice vaginal epithelium in estrogen period and had distinct promotion effect on the granular layer formation of mice tail epidermis. It indicated that Shaohuang granules are effective on treating psoriasis. It can decrease the skin thickness and improve skin lesion by affecting the epidermal parakeratosis and epidermal over-proliferation. It also can inhibit the mitosis of vaginal epithelial cells and promote the epidermal granules formation of mice tail.

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Screening and optimization of cordycepic acid extraction method of Cordyceps militaris CNZ

LIN Ai-hua, MIAO Li-yun, WANG Jun-ling, LI Hui, SONG Fa-jun, ZHANG Peng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 141-144. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.030

Abstract （365）

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In order to obtain the optimal cordycepic acid extraction method of Cordyceps militaris CNZ, six extraction methods were used to extract cordycepic acid of C. militaris CNZ, respectively, and the extraction yield of cordycepic acid with ultrasonic-associated ethanol extraction method was the maximum for 113.31 mg/g, so was selected as one suitably initial method. Subsequently, the extraction conditions, including extraction times, solid-liquid ratio, ultrasonic time and ethanol concentration, were further optimized by single factor experiments and orthogonal experiments. One optimized cordycepic acid extraction method of C. militaris CNZ was obtained as that： ethanol concentration was 20%（volume fraction）, solid-liquid ratio was 1:10（g:mL）, ultrasonic time was 20 min and extraction times was three. Using the optimized extraction method, the extraction yield of cordycepic acid of C. militaris CNZ was 161.06 mg/g, which was about 42.1% higher than that using the initial extraction method.

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Effect of ultra-high pressure treatment on the quality of fruits and vegetables

XIONG Zi, LIAO Li, QIAO Yu, CHENG Wei, SHI De-fang, WANG Jun, CHEN Xue-ling

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 145-150. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.031

Abstract （510）

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UHP（ultra-high pressure） is one of the most popular non-thermal technologies about fruits and vegetables processing in the world. The principle and features of the UHP were introduced, followed by the analysis of UHP's influence on microorganism, enzyme activity, color, texture, flavor, nutritive activity and so on. The application and prospect of the UHP were also analysed. The UHP evidently extended fruits and vegetables' shelt life with the flavor and nutrition being maintained efficiently, in the near future, the UHP would be applicated from fruits and vegetables to more extensive foods, and be industrialized gradually.

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Study on the geographical origin identification of american ginseng based on multi-element analysis and statistical methods

HOU Hao-ran, ZHOU Jin-song, LIU Guo-wei, KONG Jian, Ma En-yao, LUO Wen-ying

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 151-154. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.032

Abstract （382）

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The study aim is to investigate the feasibility of multi-element analysis in determination of the geographical origin of american ginseng, and to make a choice of determining effective tracers in the origin assessment of american ginseng. The contents of 28 chemical elements of Sc, Y, La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, Lu, Cr, Mn, Fe, Ni, Cu, Zn, As, Se, Cd, Sn, Hg and Pb were detected by ICP-MS in 20 samples from Canada, the United States, and the Northeast of China, and analysis of variance （ANOVA）, cluster analysis （CA） and discriminant analysis （DA） were applied in the data analysis. The results showed that the element contents were different in american ginseng samples from the other different locals. CA classified the samples into different categories, which was consistent to their geographical origin. Three elements of Y, Fe and Ni were screened by DA and suggested as better tracers for american ginseng origin assessment, a 100.0% correct classification was achieved. Therefore, it is feasible to determine the geographical origin of american ginseng based on multi-element analysis.

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Comparative analysis of volatile components in Guizhi Fuling bolus and other dosage forms

MA Yin-yu, LU Jin-qing, LUO Bei-bei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 155-158. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.033

Abstract （481）

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Headspace solid phase microextraction （HS-SPME） combined with gas chromatography-mass spectrometry （GC-MS） was used to analyze the volatile components of Guizhi Fuling bolus,Guizhi Fuling tablets and Guizhi Fuling capsules, the relative contents of each component were calculated. The results showed that 22 peaks were detected from Guizhi Fuling bolus, 17 components were identified, accounting for 98.41% of the volatile components, 15 peaks were detected from Guizhi Fuling tablets, 12 components were identified, accounting for 89.12% of the volatile components, and 30 peaks were identified from Guizhi Fuling capsules, accounting for 99.46% of the volatile components. The composition and content of volatile components in Guizhi Fuling bolus and other dosage forms changed.

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Determination of domoic acid in shellfish by liquid chromatography-orbitrap mass spectrometry

WU Xiang-qing, YU Tao, WU Ming-yuan, HUANG Guo-qiu, YANG Shu-li

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 159-161. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.034

Abstract （329）

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Domoic acid （DA） is a kind of neurotoxin produced by Pseudo-nitzschia diatom that can accumulate in shellfish. Consuming shellfish products contaminated by domoic acid can cause memory loss, dizziness, coma and even death. In this paper, a method was established for the simultaneous determination of domoic acid （DA） in shellfish by liquid chromatography-orbitrap mass spectrometry. Oyster and clam samples were extracted with methanol and purified with QuEChERS technology. The analytes were isolated by Hypersil GOLD C₁₈column and gradient eluted with acetonitrile-2 mmol/L ammonium formate solution （0.2% formic acid）. DA were determined by orbitrap mass spectrometry through positive ion scanning, full scan mode and data dependent scan mode. The experimental results indicated that the retention time of DA was all within 6min. The limits of quantification （LOQ） was 0.01 mg/kg. The calibration curves showed good linearity within the concentration range of 25~500 μg/L, R²=0.999 8. The average recoveries was between 90.5%~106.3% and the relative standard deviations （RSD） were less than 6.0% （n=6）. This method was easy, rapid and sensitive, and could be applied in the determination of domoic acid in shellfish.

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Design of intelligent agricultural environment monitoring system based on mobile communication

LI Xiao-ping, LIU Yuan-gang, XIONG Gang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 162-166. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.035

Abstract （414）

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There were many problems existing in environmental parameters of facilities agriculture in Yangling area Shaanxi province,we designed a kind of intelligent agricultural environment monitoring system based on mobile communication, it combined with computer, sensor, mobile communication and other technologies. The system adopts modular design method and provides hardware design and software design. We have completed the production of control system,according to the growth needs of the facility agricultural crops,automatic intelligent operation of sprinkler irrigation, ventilation, light supplement, heating and other control modes counld be achieved. The test results show that the environment data measurement of the control system is more accurate and the information transmission is normal,When the data exceeds the limit, the operation of the equipment is executed, the control performance is good and the alarm is timely,it has certain application value and promotion value.

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Study on early warning model of tomato stem rot in solar greenhouse based on internet of things technology

HUANG Yuan, YANG Ying-ru, GAO Xin-na, LI Hai-jie, WU Meng, DU Ya-ru

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 167-170. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.036

Abstract （398）

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The growth environment data of autumn tomato in solar greenhouse were collected by sensor technology of agricultural Internet of Things. Through practical research, the early-warning model of stem-based rot of autumn tomato in solar greenhouse was initially established by SPSS Statistics Subscription. The model test effect was good, and the experiment proved that the operation effect of the early-warning model was better in actual production. The establishment of the early warning model fully explored the relationship between soil humidity, air temperature and the occurrence probability of tomato stem base rot, laid a foundation for the establishment of early warning system for tomato diseases and insect pests, and made an exploration for the realization of accurate management of modern agriculture.

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The use of tissue culture to establish the rapid propagation system of Ampelopsis grossedentata

LIAO Lu-jing, JIANG Nian, SUN Zi-wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 171-176. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.037

Abstract （356）

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Stems with buds in Ampelopsis grossedentata as test materials, culture medium and hormone combination in various stages in the process of cultivation of Ampelopsis grossedentata tissue and ratio of matrix in the seedling process were studied. Conclusions are as follows： The optimal primary medium in rapid propagation system of tissue cultured seedling of Ampelopsin Grossedentata was MS+1.5 mg/L 6-BA+0.2mg/L NAA+2 g/L PVP. The optimal matrix matching for primary stem cultured callus was MS+4.0 mg/L 6-BA+2.0 mg/L ZT+1.0 mg/L NAA. The optimal proliferation medium for Ampelopsin Grossedentata bud was MS+2.0 mg/L 6-BA+1.0 mg/L NAA+2.0 mg/L ZT. The optimal strong seedling medium was MS+1.0 mg/L 6-BA+1.0 mg/L NAA. The optimal rooting medium was NAA（0.1 mg/L）+IBA（0.5 mg/L）, with the rooting rate reaching 86.7%. The optimal transplantation matrix at hardening-seedling stage was peat + perlite （2:1）, and the hardening-seedling and transplanting survival rate could reach 73% if it was watered with modified Hoagland nutrient solution.

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The coupling and coordinating relationship between land use benefit and ecological environment in Chengdu-Chongqing city cluster

YANG Xiao-han, TANG Xiang-yun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 177-181. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.038

Abstract （397）

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Based on the construction of the evaluation index system of land use benefit and ecological environment level, the weight of variation coefficient method is used to determine the weight, and the coupling coordination model is combined to reveal the time and space changes of coupling degree of land use benefit and ecological environment level coupling between Chengdu and Chongqing urban agglomerations from 2006 to 2016. The results show that the land-use benefits of various cities in the Chengdu-Chongqing city cluster show a gradual upward trend, the growth rate slows down year by year, and the internal differences are significant. The eco-environment level of various cities has basically shown a year-on-year growth trend, and a small number of cities have begun to decline after 2011. The coordination between the land use benefits and the ecological environment of the Chengdu-Chongqing city cluster needs to be strengthened. Most cities are in a state of maladjustment throughout the year, mainly with moderate and imminent disorders. The spatial variation of the coupling degree shows a trend of rising from Chengdu to the northeast of the urban agglomeration and then to the southeast.

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Risk of farmland transfer under the “three powers separation” and its prevention

LI Feng-xing, CHEN Guang-hua

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 182-185. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.039

Abstract （384）

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Agricultural and transfer was an important measure to realized large-scale intensive agricultural management, but in practice there were risks such as farmland “non-grain” and “non-agricultural”, excessive concentration of farmland transfer and forced transfer of farmland. Therefore, it was necessary to improve the risk prevention system of farmland transfer under the “Three Rights Separation” by strengthening the supervision of collective economic organizations over the use and scale of farmland transfer, constructing the farmland transfer performance guarantee insurance system, improving the reasonable pricing mechanism of farmland transfer, and cultivating new agricultural operation subjects.

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Research on legal issues of land operating right financing guarantee

GONG Peng-cheng, SUN Jia-yuan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 186-191. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.040

Abstract （453）

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Study the legal issues related to the land management right financing guarantee of the newly revised “Land Contract Law of the People's Republic of China”. The nature of land management right should be treated differently. The land management right with a circulation period of less than five years embodies the nature of creditor's rights, and the rest should be recognized as the property of usufruct. The implementation of the new law provides legal support for the financing guarantee of land management rights, but it still faces the practical problems of unknown guarantee subject, single realization method of security right, incomplete value evaluation system, and inadequate risk prevention and control mechanism. Through the improvement of legislative norms, strengthening the construction of risk prevention and control mechanism and value evaluation system, the goal of releasing rural land management rights and promoting the modernization of agricultural production is achieved.

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Urban rights in the perspective of urban-rural balance

XU Chuan-dong, HU Jie

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 192-196. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.041

Abstract （376）

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Studying the basic content of urban rights, it is believed that urban rights are the concrete manifestation of human rights in urban spaces, and we summarize the basic rights characteristics of urban rights. From the perspective of urban rights, we examine the pattern of power difference between urban and rural areas at this stage, and analyze the right gap between urban and rural areas in politics, economy, social life and environmental protection. It is proposed to realize the equal distribution of rights between urban and rural areas through the reform of the household registration system, emphasizing the role of urban rights to regulate the orderly expansion of cities, and leave sufficient development space and opportunities for rural areas. Eventually, the establishment of an equal rights system between urban and rural areas will be achieved, the harmony between urban and rural areas will be realized, and the integration of urban and rural development will be promoted.

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Research on marketing strategy of Carya cathayensis Sarg. handicraft in Shangluo city

GUO Meng, ZHANG Shuai, WANG Yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 197-200. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.042

Abstract （447）

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Based on the data of 536 questionnaires, the consumer psychology of college students on Shangluo city Carya cathayensis Sarg. handicraft is analyzed and the marketing strategy of Carya cathayensis Sarg. handicraft is discussed. The results show that consumer's product cognition, quality cognition, brand preference, novelty hunting preference, way preference, cultural preference, marketing recommendation and advertising effect have significant positive effects on purchase behavior; the price preference has positive but not significant effects on purchase behavior; the origin cognition, experience preference and family friend recommendation have negative but not significant effects on purchase behavior. Based on this, the suggestions of establishing a perfect quality inspection system to ensure product quality, designing both tradition and fashion to enhance cultural connotation, and carrying out multi-channel marketing publicity to expand product impact are put forward.

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Study on the spatial evolution trend of population in Hubei province since the period of reform and opening up

ZOU Peng, WANG Ming-jie, WU Si, WANG Qian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 201-207. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.043

Abstract （482）

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The spatial evolution law of the population in Hubei province in the past 40 years is analyzed by using the population gravity center method and the gray system model, and base on this, the population trend in the next decades to 2035 is predicted. The results show that, firstly, national policies such as family planning and market economy are the most important factors affecting the evolution of population spatial in Hubei province since the reform and opening up; secondly, Hubei province would have been in the middle stage of urbanization before 2035 with the population gathering to urban areas and urbanization still maintaining rapid development, so rural revitalization strategy is difficult to bring obvious rural population return during this period; thirdly, since the reform and opening up, the population center of gravity in Hubei province is shifting towards Wuhan,and even by 2035, Wuhan will still maintain a strong attraction to the population of the whole province, but the attractiveness of Wuhan's central urban area will be weakened, and the population growth in the suburbs will exceed that in the central urban area. The second and third conclusions further confirm the general law of urbanization.

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A new route of rural revitalization strategy in local financial service：Take establishment of agriculture-related financial leasing company as an example

LIN Bie-yu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 208-211. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.044

Abstract （358）

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The implementation of the rural revitalization strategy is an important strategy decision at the Nineteenth National Congress. During the implementation process, local finance plays a key role in funding source. Personal direct lease, enterprise leaseback and manufacturer lease, the application of three basic financing modes on the implementation of the rural revitalization strategy were studied by taking the establishment of agriculture-related financial leasing company with local financial contribution as an example. The advantages and necessity of state-owned agriculture-related financial leasing company in promoting agricultural rural development were also discussed to further looking for a new way of local financial servicing rural revitalization strategy.

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Discussion on the strategic issues of “Three Agriculture”

REN Yi-jie, LI Jing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (9): 212-216. DOI: 10.14088/j.cnki.issn0439-8114.2020.09.045

Abstract （375）

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The contemporary strategy of “Three Agriculture” should focus on the demands of the times to build the reform path, coupling the mechanism of the problem to explore key strategies, and practice the specific tasks in combination with the theoretical innovation and planning requirements, to design the content and requirements of the “Three Agriculture” strategy in the new stage. These have become three new directions for deepening the reform, including the need to strengthen the development of rural finance, the need to integrate rural industry, and the need to improve the education level of the rural population. The new ways to revitalize rural development around solving the existing problems are put forward.

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Research advances in biological control of tea pests in China

LI Fei, YANG Dan, ZHENG Jiao-li, YAO Jing-wu, ZHU Zhi-gang, HUANG Da-ye, CAO Chun-xia

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 5-9. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.001

Abstract （484）

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The effects of chemical pesticides on controlling main tea pests is rapid, but with its abuse use, the “3R” problems has produced directly affecting the sustainable development of tea industry. With the tea quality and safety issues are increasingly concerned, biological control as an indispensable part of integrated pest management has turned into a broad application and development prospect in the tea pests control system. The occurrence and characteristics, the application of the biological control, the drawbacks of the biological control are summarized, and the prospect of the biological control of the main tea pests are put forward.

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Research on plant configuration in healing landscape

MA Li-nan, XU Ya-ru, ZHAO Zhong-jian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 10-14. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.002

Abstract （401）

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On the basis of the healing effect of human physiology and psychology through plants, and according to the proportion of healthy plants in all plant species, the rehabilitation landscape was divided into two types, plant-oriented healing landscape and plant-auxiliary healing landscape. Taking these indexes as the basis of plant allocation, which were healthful plants account for a large proportion, plant growth habits, people-oriented, plant diversity, landscape beauty and plant safety, three kinds of healing landscape plant configuration schemes that suitable for pension community, general hospital and community garden were put forward respectively. And the needs of plants in healing landscape, the selection, seasonal phase, growth habits and functions of plants and the advantages of allocations were described.

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Application of mind mapping in life science research

WANG Chao, SHI Fu-mei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 15-18. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.003

Abstract （474）

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With the development of science and technology, there are increasing number of infiltration and mergence between life science and other scientific branches, and the information and data that life science researchers are facing with are becoming more and more complicated. As a graphics thinking tool that expressing divergent thinking, mind mapping can help life science researchers to manage data and information effectively, arrange schedules and quickly organize thoughts to write papers, etc. Here combing with the characteristics and the trend of future development of life science, we summarize the application of mind mapping in life science research, aiming to offer help for life science researchers to use mind mapping software to manage scientific data and information.

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The preliminary study of the genetic mechanism of recessive nuclear three-lines maintainer in Brasscia Napus L.

LIU Nian, MENG Da-qing, ZHU Jie, XU Kai, TANG Tian-ze, TU Jin-xing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 19-22. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.004

Abstract （411）

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The new nuclear three-lines maintainer Mian 7MB was revealed by Mianyang Academy of Agricultural Sciences in 1993. In the segregating population of selfing and sibling offspring, the proportion of fertility segregation did not conform to Mendelian genetic law, showing a partial segregation phenomenon. Through the field genetic analysis and cytological analysis, the genetic mechanism of Mian 7MB was preliminarily explored. The results showed that the segregation distortion in Mian 7MB only happened in the male gamete transference. Mian 7MB segregation was not due to abnormal meiosis, but may be due to the death of zygotes of some genotypes, resulting in abnormal proportion of offspring.

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Current situation and analysis of crop germplasm resources in Zhushan county of Hubei province

XIONG Jun-bo, LU Jiao-yun, TIAN Hong, ZHANG He-shan, JIAO Chun-hai, LIU Yang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 23-28. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.005

Abstract （431）

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Based on the results of crop germplasm resources in 1956,1981 and 2014 of Zhushan county in Hubei province,the changes of agricultural industry structure, crop varieties and plant area in recent 60 years are conducted. By analysis of the present situation in the protection of crop germplasm resources, the corresponding countermeasures and suggestions are put forward in order to provide a reference for the protection and utilization of crop germplasm resources in Zhushan county.

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The interaction effect of potato intercropping in young apple orchard

ZHANG Biao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 29-31. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.006

Abstract （380）

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Two and three-year-old apple trees were used as the test materials, and the two kinds of width of nutrition zone (1.9 and 2.5 m) was set up. Through the interaction test of two factors, the interaction effect of fruit trees and potatoes in different intercropping modes of young orchard was studied. The results showed that for 2-year-old fruit trees, different width of nutrition zone had no significant effect on the growth of fruit trees, but had significant effect on potato yield, when the width of nutrition zone was 1.9 m, the yield of potato was significantly higher than that of 2.5 m. Therefore, the optimum intercropping density for 2-year-old orchard was 0.70 m×0.35 m. For 3-year-old fruit trees, when the width of nutrition zone was 1.9 m, the yield of potato was significantly higher than that of 2.5 m, but the plant height, trunk diameter and branching number of fruit tree were significantly smaller than that of 2.5 m. Potato intercropping in young orchard needs to ensure the growth of fruit trees. Therefore, intercropping potatoes in 3-year-old apple orchard, the best intercropping density was 0.50 m×0.35 m.

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Analysis of drought and flood change based on standard precipitation index(SPI) and its impact on lake water level of Honghu lake

DENG Yan-jun, ZHOU Shou-hua, YANG Chao, XIA Zhi-hong, LIU Kai-wen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 32-37. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.007

Abstract （402）

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Based on the monthly precipitation data from Honghu meteorological station and water level data from Honghu lake, variables time-scale standard precipitation index(SPI) was calculated. Using SPI, the changes of drought-flood and the temporal characteristics of drought-flood events were analyzed in Honghu lake. The correlation among precipitation, various time-scale SPI and water lever were investigated. The results showed that the frequency and grade of the drought and flood events in Honghu lake in the past 62 years were approximate on the annual scale, little difference on seasonal scale. There was a wetting trend in Honghu, and annual SPI decreased with an average rate of 0.086/10a. SPI declined in spring and autumn, and slightly intensified in drought; SPI increased in summer and winter, and increased in flood and waterlogging. The variability between the water level and the precipitation data was consistent, but the water level later than the precipitation data three months. Variables time-scale SPIand water level were significant correlation. The 3 month scale SPI₃ was most closely correlated with water level of Honghu lake. The 3 month scale SPI₃ agrees well with the variability of the water level. SPI₃ can be used to monitor the water level of Honghu lake.

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Characteristics of high-influence weather variation and its influence on agriculture in Ezhou city in the recent 60 years

HUANG Si-xian, WANG Bao, CHEN Zheng-hong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 38-43. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.008

Abstract （310）

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:Based on the climatic data of Ezhou city from 1959 to 2018, the evolution characteristics of six types of high-influence weather in recent 60 years, including frost days, snow days, low temperature days, high temperature days, rainy days and rainstorm days are analyzed by means of linear trend method, Mann-Kendall mutation test and Morlet wavelet analysis. The results show that the decreasing trend of frost days, snow days, low temperature days and rainstorm days in Ezhou city is obvious since the 10s of the 21st century, and the increasing trend of high temperature days and rainstorm days is obvious. In the past 60 years, the number of frost days and low temperature days showed a significant downward trend; The number of high temperature days showed a significant increase trend; The number of snow days, rainy days and rainstorm days showed a steady and slightly increased trend. The number of frost days is the most in January and December; The number of low temperature days and snow days is the most in January; The number of high temperature days is the most in July; The number of rainy days is more from March to May in spring, less from September to November in autumn; And the number of rainstorm days is the most in June. The number of frost days, snow days, low temperature days, high temperature days, rainy days, rainstorm days mutated in 2010, 2001, 1981, 2005, 2000 and after 2015, 1968, respectively. The number of frost days has a quasi 20 year interdecadal cycle in the whole time domain; The number of days with snow cover from 1964 to 2014 has a quasi 20 year interdecadal cycle; The number of low temperature days has a 18~22 years interdecadal cycles in the whole time domain; The number of high temperature days has a quasi-16-year interdecadal cycle and 4~6 years annual cycle in the whole time domain. The number of rainy days has a quasi-22-year interdecadal cycle and quasi-8-year annual cycle in the whole time domain. The change of high-influence weather can produce favorable and unfavorable effects on agriculture in Ezhou city.

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Comparative analysis of two heavy rainfall weather processes in winter flood season in Hunan province

HUANG Juan, FAN Shao-jia, ZHOU Li, WANG Mi-ji

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 44-52. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.009

Abstract （350）

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Based on the NCEP/NCAR reanalysis data with resolution of 1°×1° and from four aspects of circulation situation, water vapor, dynamics and unstable stratification, a comparative analysis of the two rainstorms in Hunan province in November 2015 is maked. The results show that the two rainstorms are related to the abnormal large-scale circulation background. The province large area and strong intensity of subtropical high are beneficial for water vapor to converge into Hunan province along the southwest side of subtropical high. The intensity of polar vortex is stronger than the annual average, which makes the intensity of cold air stronger. The strong cold air in the south intersects with the warm and wet air in the north, causing the heavy precipitation weather process. Ground inverted trough and low-level eddy shear are the main influence systems of the two heavy precipitation processes. In process 2, the ground inverted trough has strong intensity, wide range and slow movement, and corresponds to the low-level eddy shear intensity. In process 1, the ground inverted trough and the low-level eddy shear intensity, range and duration are lower than those in process 2. The water vapor of 700 hPa is the main transport source of the two regional rainstorms. The water vapor of process 1 is mainly from the South China Sea and process 2 is from the Bay of Bengal, and the water vapor condition is stronger than that of process 1. In process 1, the vertical upward motion zone extends upward at a higher altitude, with deep convergence layer in the lower layer, strong frontal zone and unstable energy accumulation. In process 2, stable precipitation is dominant, vertical upward motion, convergence and divergence are shallow, mainly concentrated in the middle and low levels.

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The characters of spring snow disaster and corresponding atmospheric circulation over the eastern Tibet during 1979―2014

Luosangwangmu, Danzengzhaba, DA Qiong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 53-57. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.010

Abstract （376）

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Using a variety of in-situ meteorological elements datas such as snow depth and NOAA atmospheric circulation data, the frequency of spring snow disasters at 27 stations in the eastern Tibet and corresponding atmospheric circulation anomalies during 1979―2014 were analyzed. The results showed that there were 33 snowstorms in 27 stations in eastern Tibet during 1979―2014. The snowstorm was mainly concentrated in the area between the Tanggula mountains and the Nyenchenthanglha mountains and the southern part of Shannan; The greater the maximum snow thickness and the total snowfall during the snowstorm, and the greater the average snow thickness of the snowstorm. The greater the relative humidity, the greater the average snow thickness during the light-level disaster. The greater the wind speed, the greater the average snow thickness during the light-level disaster and the middle-level disaster; During the light-level disaster and the middle-level disaster, there were “+-+-+” and “+-+-+-” wave train propagating from the Atlantic to the western Pacific through the high latitude. During the severe-level disaster, the intensity of the North Atlantic negative geopotential height anomalies was stronger than the one during the light-level disaster and the middle-level disaster, and there was a "+-+-+-" wave train propagating from the Atlantic to the western Pacific through the mid-latitude.

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Optimizing water and fertilizer system of cucumber in solar greenhouse based on the different treatment of water and fertilizer

WANG Rong, MA Ling, YANG Chang-xin, HUANG Ling-dan, LI Jia, YANG Dong-yan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 58-63. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.011

Abstract （384）

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By studying the effects of coupling irrigation amount and fertilizer amount on cucumber growth index, fruit yield and quality, the integrated management system of water and fertilizer in greenhouse cucumber in dry farming area of Ningxia is explored. Two factors and three levels orthogonal test of irrigation amount and fertilizer amount were set with spring and summer stubble cucumber as test material. The results show that the cucumber height shows an available logistic equation simulation during whole growth period, and almost all simulation coefficients reaches as high as more than 98%, plant height show TR7 (high water and fertilizer)﹥TR8(medium water and high fertilizer)﹥TR4(high water fertilizer), and this three treatments are significantly higher than those of other treatments; From the peak of fruit stage to the end of fruit stage, the stem diameter almost stopps growing. At the same time, high fertilizer level can promote growth of leaf, the amount of water and fertilizer is positively correlated with plant yield, the correlation coefficient is up to 0.47~0.57, and the amount of water and fertilizer is positively correlated with yield. Under the condition of sandy loam (bulk density is 1.10 g/cm³, field water capacity is 24.21%), the best treatment is 2 222.05 m³/hm² of irrigation water and 1 200 kg/hm² of fertilizer. The yield is up to 110 190.00 kg/hm², the economic benefit is up to 192 700 yuan/hm², the water productivity is 49.58 kg/m³, and the fertilizer productivity is 91.83 kg/kg. Among them, N amount is 216 kg/hm², P₂O₅ amount is 108 kg/hm², and K₂O amount is 324 kg/hm².

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Effects of different fertilization methods on the growth and development of flue-cured tobacco and the yield and quality of tobacco

WANG Yi, GUAN En-sen, CHEN Hong-wei, WANG Shu-ke, GAO Kai, WANG Yu-hua, WANG Da-hai, ZHANG Xu, LIU Chun-ju, YANG De-lian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 64-68. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.012

Abstract （412）

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A field experiment was designed to study effect of different fertilization mode on development of tobacco, yield and its quality. The results showed that compared with other fertilization treatments, potassium nitrate as topdressing and other fertilizers as basal fertilizer had the best comprehensive performance. Compared with the application of all fertilizers as basal fertilizer, potassium nitrate as topdressing can promote the development of tobacco plants in the growing and flat-topped periods, and the tobacco yield and output value are increased by 12.80% and 9.70%,respectively. The color, oil content and total score of middle and upper tobacco leaves increased by 2.50%~3.90%, 4.83%~9.43% and 1.68%~2.86%, respectively. The potassium content of middle and upper tobacco leaves increased by 14.29% and 16.89%, and the internal chemical composition of tobacco leaves was more harmonious.

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Zooplankton community structure in relation to environmental factors in the Shanghetang reservoir

XU Yong-bin, GUO Ai-huan, HE Wen-fang, YUAN Ju-lin, LI Ming

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 69-74. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.013

Abstract （413）

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The quarterly investigations were carried out on zooplankton community in Shanghetang reservoir in 2016. The species composition and seasonal changes of zooplankton in the Shanghetang reservoir were analyzed. The correlation between the zooplankton community structure and the environmental factors was discussed. A total of 19 genera and 29 species of zooplankton species were observed. The dominant species, average density and biomass of zooplankton community displayed obvious variation with season. Dominant species in the Shanghetang reservoir were Trichocerca pusilla, Brachionus diversicornis and Polyarthra vulgaris. The average annual values for zooplankton density and biomass were (1 225.88±1 572) ind./L and (0.34±0.415) mg/L, respectively. The species richness index(J) and Shannon-Wiener index (H') also showed obvious seasonal variations, with the highest values both in autumn. From the result of canonical correspondence analysis, environmental factors such as water temperature, SD and pH affected the composition and the density of zooplankton community.

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Ammonia-magnesium phosphate precipitation degrades the trace ammonia nitrogen in water

ZHAO Ye-jun, WANG Huan, LU Ying-hua, WANG Ying-ying

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 75-76. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.014

Abstract （359）

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The degradation of trace ammonia nitrogen in water by ammonia-magnesium phosphate precipitation method was studied, and the effects of reaction time, NH₄⁺/PO₄^3-/Mg²⁺ molar ratio and other factors on the degradation of ammonia nitrogen were discussed. The results showed that when 1 L of ammonia nitrogen in water when the initial concentration of 2 μg/mL, to optimize the reaction conditions as follows: Input N, P, Mg of the mole ratio of 1∶1.1∶1.4, the reaction time of 90 min, the water ammonia nitrogen removal rate can reach 78.12%. When it was applied to the ammonia nitrogen degradation of Jiuqu river, the degradation rate of ammonia nitrogen in water was reduced to 70.02% due to the various impurities in the water.

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Studies on mutagenesis of Bacillus velezensis CY30 with atmospheric and room temperature plasma

LIU Fang, LIAO Xian-qing, ZHOU Rong-hua, RAO Ben, CHEN Wei, HUANG Da-ye, WAN Zhong-yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 77-80. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.015

Abstract （358）

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Atmospheric and room temperature plasma(ARTP) was used to mutate Bacillus velezensis CY30 for improving the production of spores and fungicidal activity. The most optimal condition for the ARTP mutagenesis was working flow rate at 10 L/min, treating distance of 2 mm and electrical power of 100 W. The mutants were selected based on microscopic morphology of the colonies, the spore counts and antifungal activity of the cultures from shake-flask fermentation. A mutant with code of CY30-314 was obtained through 3 rounds of microscopic checking of the slants and shake-flask fermentation. The spore counts of the mutant in shake-flask fermentation was 12.23 billion spores/mL, and the corresponding cultures inhibited the growth of fungus for 75.58%. The mutant also showed genetic stability. The mutant produced 25.3% more spores in shake-flask fermentation which resulted in the increase of antifungal activity at 15.6%. The duration of the mutant was shorted for 2 h.

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Predation of Harmonia axyridis Pallas adults to Megoura japonica Matsumura

WANG Hai-nuo, GENG Shuo, YI Chao-qun, CHEN Jia-jie, WU Yu-lun, LIU Ting-hui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 81-83. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.016

Abstract （356）

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Under laboratory conditions, a preliminary study of Harmonia axyridis Pallas of the male and female adult Megoura japonica Matsumura feeding effect was researched, using the Holling-Ⅱ equation function response model of Harmonia axyridis of male and female adults feed on Megoura japonica Matsumura fitting. The results show that the Harmonia axyridis Pallas are accord with the function of the male and female adult reaction Holling-Ⅱmodel. However, in a certain space and at the same proportion of prey, the intra-species interference would have an impact on the prey of adult ladybirds of Harmonia axyridis Pallas preying on the Megoura japonica Matsumura. In this experiment, the interference coefficient was 1.09.

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Effects of pinoxaden on the control of winter wheat field grass weeds and on wheat growth

ZHU Wen-da, WU Jia-jia, LI Lin, SONG Zhao-xin, LIU Quan-ke, ZHU Jia-hong, YAN Dong-dong, WANG Qiu-xia, LI Yuan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 84-86. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.017

Abstract （400）

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Through field efficacy tests, the field control effects and safety of 5% pinoxaden EC on winter wheat field grass weeds were evaluated, the optimal use period, method and dosage for the pesticide were determined. The results showed that the 5% pinoxaden EC had a good effect in controlling Alopecurus aequalis and Beckmannia syzigachne (Steud.) Fern., and it was advisable to use effective components of 45.0~60.0 g per hectare. The suitable period of application was the best effect in the 3 to 5 leaf stage of grass weeds. Spraying with 450 litres of water per hectare can effectively control weeds in wheat fields. Within the test dose range, all test agents had no adverse effects on wheat growth, and no adverse effects were found on the surrounding environment and other beneficial organisms in the test area.

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Effects of fluroxypyr on controlling of broad-leaved weeds in wheat field and on nutrient water content

ZHU Wen-da, WU Jia-jia, LI Lin, SONG Zhao-xin, ZHU Jia-hong, YAN Dong-dong, WANG Qiu-xia, LI Yuan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 87-88. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.018

Abstract （410）

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In order to verify the effects of 20% fluroxypyr EC on controlling weeds in wheat fields, 20% fluroxypyr EC for controlling weeds in wheat fields was tested. The results showed that the comprehensive plant control effect of weed control was 88.50%~95.01%、78.72%~85.13% 30 days and 40 days after application, respectively; The comprehensive fresh weight control effect was 85.57%~94.35%.20% fluroxypyr EC can reduce the uptake of nitrogen, phosphorus and potassium by field weeds and maintain soil fertility.

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The susceptibility test of dinotefuran to rice brown planthopper (Nilaparvata lugens)

HUANG Jian-hua, YANG Ren-feng, YANG Ying-qing, LAN Bo, LI Xiang-min

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 89-91. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.019

Abstract （367）

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Aiming to determine the field control effect and dosage of 20% dinotefuran SG on rice brown planthopper [Nilaparvata lugens (Stal)], spraying the leaves of rice seedlings by cage method, the control effects of 20% dinotefuran SG against rice brown planthopper after the drug 2~7 days was investigated. The control effects of 20% dinotefuran SG at the concentration of 60~600 g/hm² were similar to that of 25% thiamethoxam WG 60~120 g/hm², which were better than that of 50% pymetrozine SG 300~600 g/hm² and 10% imidacloprid WP 300~600 g/hm². The recommended dosage of 20% dinotefuran SG was 60 g/hm² in the field.

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Study on the genus Microchironomus, Kieffer from Zhejiang province

WANG Yu-ting, CHEN Xin-yi, ZHANG Yu-qing, LI Ye-le, QI Xin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 92-93. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.020

Abstract （430）

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The genus Microchironomus Kieffer from Zhejiang province including 2 species, [Microchironomus tener (Kieffer, 1918) and Microchironomus tabarui Sasa, 1987] were reviewed. The adult male of Microchironomus tabarui was re-described and figured, which was the new record for Zhejiang province.

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Study on application of mechanical control and maintenance technology for thin slice raw materials

HU Wan-hong, LIU Yong, WU Min, JIANG Wen-juan, LIN Jing, DING Yu-qiong, WU Bin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 94-96. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.021

Abstract （348）

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The effect of nitrogen-filled on the control of adult and larvae of the thin slice raw material Lasioderma serricorne was studied. At the same time, the application of mechanical nitrogen-filling regulation in wafer curing was studied. The results showed that under certain technical conditions, the oxygen concentration in the sealed soot was less than 2% for 45 days, and the mortality of tobacco adults and larvae in the tobacco could reach 100%. The mechanical nitrogen filling regulates the water content of the thin slice raw material. The mechanical nitrogen filling control had obvious function to adjust the moisture content of sheet material, and the test cargo had no mildew or carbonization.

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Study on the growth law of the new red flesh kiwifruit cultivar Hongshi No.2 fruit

ZHANG Qian, LI Ming-zhang, WANG Li-hua, LIU Xin, FAN Gao-min

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 97-100. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.022

Abstract （466）

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:The indexes of fruit vertical and horizontal diameter, single fruit weight, dry matter, soluble sugar content, fruit firmness and fruit flesh color were tested and analyzed at 26~182 days after full bloom for the study on the growth law of the new red flesh kiwifruit cultivar Hongshi No.2. The results showed that Hongshi No.2 growed in a single “S” curve, fruit development was divided into three stages, cell division period(60 d after full bloom), fruit inflating stage(60~144 d after full bloom), fruit mature period(144~159 d after full bloom). The growth and development rules of Hongshi No.2 kiwifruit were found out, which provided a basis for the formulation of supporting production technology and the determination of timely harvesting period.

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Effects of fertilization on the cold resistance of Chinese rose base on conductivity method and the Logistic equation

WANG Li-ting, WU Hai-dong, YANG Pan, GUO Feng-min, SUN Gui-qin, WANG Sheng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 101-104. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.023

Abstract （385）

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Using 4 Chinese rose(Rosa chinensis) varieties Schloss Mannheim, Carefree Wonder Shrub, Eiko and Black magic as materials,the changes of electrolyte extravasation rate of leaves under different low temperature stresses after nitrogen fertilization were studied,in order to determine the influence of fertilization on the cold resistance of Chinese rose. Logistic equation was used to determine their semi-lethal temperature(LT₅₀). The results showed that with the temperature decreased, the electrolyte extravasation rate of the four varieties showed a S-shaped cure, the LT₅₀ of Schloss Mannheim, Eiko and Black magic treated with nitrogen fertilizer were lower than that without nitrogen fertilizer.

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Breeding and application of new sugarcane varieties Qiantang 8 with high yield, high sucrose and resistance

LEI Chao-yun, LEI Shi-fu, XIE Hui-jue, RAO Ping, LEI Jing, BAN Xiu-zhi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 105-106. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.024

Abstract （385）

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Qiantang 8 was selected by Guizhou Institute of Subtropics Crops in 2007, and the hybrid combination is Neijiang 00/118(♀) and Guitang 94/119(♂). This new sugarcane varieties with high yield, high sucrose and resistance was selected successfully by sugarcane cross breeding program. It is characterized with late maturity and middle size, seedling emergence rate 38.6%, tillering rate 54.0%, plant height 299.1 cm, stem diameter 3.05 cm, active stems per hectare 77 262, sucrose content 13.7%, yield 148 327.5 kg/hm², the sucrose content was 0.2% higher than that of the control variety(ROC22), and yield of Qiantang 8 was increased by 34.4% compared with control variety(ROC22). The variety has wide adaptability, strong resistance to inversion, good roots, high yield and stable yield. In 2016, it was approved by Guizhou Provincial Committee of Crop Variey Examiration and Approval, named Qiantang 8.

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analysis Changes of conventional chemical components of flue-cured tobacco leaves in Hubei province in recent 10 years

CAO Jing-lin, CHENG Jun-qi, LI Ya-pei, WU Cheng-lin, ZHANG Jun-jie

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 107-110. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.025

Abstract （383）

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In order to explore the stability and annual change trend of the chemical composition of flue-cured tobacco in Hubei province tobacco area, five representative villages were selected from 2010 to 2018 for fixed tobacco planting. The flue-cured tobacco variety K326 and unified cultivation technology measures were adopted for tobacco production. C3F tobacco samples were collected from each pilot after the end of each year for analysis of the conventional chemical composition and the change trend was analyzed. The results showed that the content of the main chemical components of the tobacco leaves in Hubei province was in a suitable range, and the content of various chemical components in the tobacco leaves in different regions fluctuated with the change of the year, and the content of the same chemical components in the same year was different in different regions. Among them, the nicotine content fluctuated greatly, followed by sugar content, while the fluctuation of potassium content and total nitrogen content was relatively small.

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Study on root growth of three common road greening plants

LI Ling-li, YU Zhi-yong, ZOU Shi-hui, WANG Bao-ning, ZHOU Li

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 111-115. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.026

Abstract （396）

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Ficus virens var. sublanceolata, Ficus microcarpa L. and Rhododendron pulchrum Sweet were taken as test objects, 1 m ×1 m ×1 m soil profile in south direction was dug to observe the distribution of the root system. It was found that from trunk at a distance of about 1 m in south direction, above 88.1% of the roots mainly distributed in the 0 to 60 cm deep soil layer for Ficus virens var. sublanceolata, above 92.0% of the root system was mainly distributed in the 0 to 60 cm deep soil layer for Ficus microcarpa L., above 43.4% of the root system was mainly distributed in the 0 to 30 cm deep soil layer for Rhododendron pulchrum Sweet. In addition, fibrous roots of the three plants can develop into flat or cloud-like structures in the gaps between purple soil fragments and bricks, and their root structure characteristics are more suitable for the status quo of landscape greening soil in Chongqing city.

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Mechanism of fat depositing caused by gossypol in liver of laying hens

WANG Qian-qian, QIN Wei-hong, CHEN Da-wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 116-119. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.027

Abstract （387）

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This research was conducted to investigate the effects of free gossypol on fatty acid in liver, lipid peroxidation index and serum index of laying hens. A total of 360 healthy 42-week-old Beijing pink 2 laying hens were randomly divided into 4 groups with 3 replicates per group and 30 hens per replicates. Laying hens in 4 groups fed the basal diet supplemented with gossypol acetate 100 mg/kg (group L), 200 mg/kg (group M), 400 mg/kg (group H) and 0 mg/kg (group C). The experiment lasted for 42 days. TG, CHOL, AST, ALT, LDL-C and HDL-C were determined with biochemical analyzer. At the end of the test, laying hens were executed with bleeding, the livers were collected to determine the fatty acids and lipid peroxidation, also pathological and ultrastructure observation. The results showed that the activity of AST, ALT increased with exposed to Gossypol acetate, showing the time and dose effect. At the 4th and 6th week, TG and LDL-C have increased very significantly (P<0.01), TC and HDL-C also increased significantly(P<0.05). Gossypol acetate decreased the unsaturated fatty acid composition and increased the saturated fatty acid proportion in liver. Liver lipid peroxidation index SOD, GSH-Px and GSH decreased significantly(P<0.05) and MDA increased significantly(P<0.05). Gossypol acetate caused laying hens hepatic steatosis, and showed a dose effect. Gossypol acetate caused structural damage of mitochondria in liver of laying hens. In conclusion, gossypol acetate caused the oxidative stress damage in hepatocyte, further caused the structure and function damage of mitochondrial which played an important role in fat depositing in liver of laying hens.

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Establishment of seed batches of avian infectious bronchitis virus

WANG Hong-cai, WANG Hui, WANG Hong-Lin, WEN Guo-yuan, ZHNAG Teng-fei, LUO Ling, LU Qin, ZHANG Rong-rong, LUO Qing-ping

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 120-122. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.028

Abstract （440）

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In order to ensure the quality of infectious bronchitis antigen, the EID₅₀ of infectious bronchitis virus strains from three sources were determined and the virus strains with high titer as virus species were selected, and the seed batch of infectious bronchitis virus WH strain were established. The poison value of freeze-dried seed poison was determined. The results showed that there was no significant difference in the poison value of toxic allantoic fluid before and after freeze-drying, and the EID₅₀ of the virus had no change within 10 generations. The poison value of freeze-dried seed poison remained basically unchanged when it was stored at -40 ℃ and -15 ℃ for one year. The seed batch was not contaminated by bacteria and other microorganisms.

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Study on extraction technology optimization and content determination of total flavones in Embelia parviflora Wall

LU Sen-hua, LIU Jia-ming, ZHANG Feng-yi, CHEN Yong, ZHANG Zhi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 123-126. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.029

Abstract （419）

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To establish the extraction technology and content determination method of total flavonoids in Embelia parviflora Wall. On the basis of single factor experiment, the optimum extraction technology of total flavonoids from Embelia parviflora Wall was optimized by L₉(3⁴)orthogonal test, and the content of total flavonoids in Embelia parviflora Wall was determined. Results showed that the extraction solvent concentration, the ratio of material to liquid, and the extraction time all had significant effects on the extraction rate of total flavonoids from Embelia parviflora Wall. The optimum extraction technology of total flavonoids from Embelia parviflora Wall was material-liquid ratio of 1:200, and reflux extraction at 100 ℃ in water bath for 120 min. The linear relationship of rutin concentration was good in the range of 9.16~54.96 μg/mL(r=0.999 8). The average recovery was 97.6%(n=6), and RSD was 2.2%.

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Determination of lambda-cyhalothrin in grape by GC-MS/MS

CHEN Ting, XU Yan-li, ZHANG Xin-zhong, WU Fu-xiang, PENG Tao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 127-130. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.030

Abstract （586）

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GC-MS/MS was used to determine lambda-cyhalothrin in grapes. After acetonitrile extraction, the samples were salted out with QuEChERS, nitrogen was blown to near dryness, and hexane was dissolved by vortexing and then detected by GC-MS/MS. The results showed that lambda-cyhalothrin had a good linear relationship in the concentration range of 0~800 ng/mL (sample content 0~1.0 μg/g), the correlation coefficient(r)>0.995 0, the detection of lambda-cyhalothrin The output limit was 2.43 ng/g, and the recovery rate was 87.3%~93.8%. The purification method and the volume of the solution after nitrogen improved the recovery rate of cyhalothrin. Select the QuEChERS Fatty sample purification kit. The recovery rate of the solution volume after nitrogen blowing was 0.1 mL, the recovery rate was 95.62%.

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Determination of ammonia nitrogen in water by molecular fluorescence spectrometry

YU Bo, XU Xing-xing, MAO Bo, LI An-di, CAI Man, LIU Xiao-xia

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 131-133. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.031

Abstract （497）

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Ammonia nitrogen in water was determined by molecular fluorescence method. Fluorescent substances were formed based on the reaction of phthalaldehyde (OPA) with ammonia nitrogen in boric acid buffer. Under certain pH value, suitable temperature and static time, fluorescence of fluorescent substances was were determined at 425 nm with 362 nm as excitation wavelength. The effects of the ratio of sodium tetraborate, sodium sulfite and OPA, temperature and time on the fluorescence value of the derivatives were investigated. The results showed that there was a good linear relationship between the amount of derivatives and the fluorescence intensity in a certain concentration range. This method has high sensitivity and is suitable for the determination of ammonia nitrogen in low concentration water samples. It could also be used for the determination of high concentration water samples after simple dilution.

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The optimization and application based on the control of tobacco leaf structure

WANG Peng-ze, DANG Xia, WEI Hui-qin, LI Yan-zhou, LYU Yao-yin, JIA Tao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 134-138. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.032

Abstract （357）

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Aiming at the demand change of tobacco industrial enterprises for tobacco leaf stalk shaped structure, after the increase of blade screen rebeating equipments behind the belt of threshing and pneumatic separation, as well as the optimization of technological parameters of the equipment, the purpose of reducing the large and increasing the medium shape of tobacco leaf structure and improving the leaf shape is realized. Taking Zhongyan 100 as the material and using the orthogonal experiment design of L₉(3⁴), the influence of three factors on the tobacco blade structure, such as frame column shape, screening roll speed and roll speed, is studied. The results show that the application of tobacco leaf screening and rebeating process can significantly improve the proportion of tobacco leaf structure after threshing and pneumatic separation. The optimal parameters for regulating tobacco leaf shape structure are hexagonal frame bar, screening roll speed of 80 r/min and roll speed of 650 r/min. By using the optimal technological parameters, the large shape of tobacco leaf decreases by 10.70 percentage points, the medium shape of tobacco leaf increases by 8.57 percentage points, and the best proportion of tobacco leaf shape increases by 12.64 percentage points. At the same time, the leaf shape tends to be circumferential, which improves the utilization rate for the silk of tobacco blade raw material.

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Isolation and identification of lactic acid bacteria and changes of physical and chemical factors in the process of Pyropia yezoensis mixed fermentation

WANG Xue-ning, ZHU Xin-ying, CHENG Qun, WANG Wei-bing, XU Yong-zhong, WANG Hong-bin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 139-144. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.033

Abstract （396）

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Three strains of lactobacillus were isolated and identified from fermented liquid of pickle after isolation and identification. They were identified as Lactobacillus brevis, Lactobacillus plantarum and Lactobacillus casei. Then these lactic acid bacteria were used to directly ferment Pyropia yezoensis with four different ratios, and some physical and chemical changes in the fermentation process was discussed. The results showed that all four kind of mixed lactobacillus fermentation broth had similar pH decline trends, from 4.51 to 2.90; 1∶1∶2 (V∶V∶V) mixed lactobacillus fermentation broth had the strongest acid production capacity, and at the end point of fermentation, the total acid content was 13.75 g/L, which was 6 times of the beginning; the total sugar content of 1∶1∶2 mixed lactobacillus fermentation broth was from the initial 29.95 g/L to 18.97 g/L at the fermentation end point; the minimum peak of nitrite content of 1∶1∶2 mixed lactobacillus fermentation broth was 3.75 mg/kg; the higher amino acid content occurred in 1∶1∶1 mixed lactobacillus fermentation broth, with the maximum value of 58.16 μmol/mL; the 1∶1∶3 mixed lactobacillus fermentation broth had the largest decrease of protein content.

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Design of coal mine geological disaster monitoring system based on WSN

SONG Wen-bo, WANG Jian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 145-149. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.034

Abstract （353）

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:In view of the frequent occurrence, strong destructive power and difficult monitoring of coal mine geological disasters, the WSN based coal mine geological disaster monitoring system was proposed. The system used Zigbee module to build wireless sensor network, and MPU-9250 (nine axis motion sensing module), soil temperature and humidity sensor, rainfall sensor and other equipment, which could effectively monitor landslides, ground collapse, ground cracks and other disasters. Wireless network data could be transmitted to the system monitoring platform through NB-IOT gateway, which was convenient for remote real-time monitoring of coal mine area. The application showed that the system has the characteristics of high cost performance, low delay, easy expansion and high accuracy, which could play a greater role in the monitoring of geological disasters in coal mines.

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Application of decision tree association algorithms in rural college students information system

FU Rong-hua

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 150-153. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.035

Abstract （407）

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By using data mining technology to analyze the relationship between these data, The hidden students' learning and living conditions in these records were founded. A comprehensive analysis system was developed, which introduces data mining technology, including decision tree algorithm and association rule mining algorithm. By analyzing the records of college students' libraries and consumption from the countryside, as well as the data of curriculum achievements and psychological tests completed by rural students, the system can excavate the life and learning status of rural students and their correlation, and display the results, so that schools or teachers can understand their status in time and clearly, which is help fulfor better teaching.

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Challenges and countermeasures of cross-province national arable land replenishment system

HUAN Ji-e, LIU Dong-hao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 154-158. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.036

Abstract （326）

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In order to maintain food security, the state proposes to "stick to the red line of 1.8 billion mu of cultivated land." However, the structural shortage of cultivated land resources in China is still very serious. On the one hand, economically developed provinces need a large amount of construction land, and there are insufficient reserves of cultivated land, and it is difficult to achieve a requistion-compensationg balance of cultivated land in the region; on the other hand, provinces in the central and western regions have relatively sufficient reserve arable land resources, but they cannot exert the maximum economic benefits. The introduction of a cross-province farmland requistion and compensation policy is an important measure to deal with this structural shortage. By analyzing the policy background, functional expectations and implementation status, it is understood that the operation of the system may face challenges, such as the lack of clear legal basis, the high cost of central government supervision information, the intensification and solidification of uneven development among regions and the crisis of “resource curse”. It can be considered by supplementing laws and regulations and using contract tools for governance; stimulating local government autonomy, using information platforms; strengthening agricultural land control, orderly development of land space; and establishing comprehensive ecological compensation mechanisms to deal with it.

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Equity principle and property right definition in grassland confirmation of village B in Inner Mongolia

HE Sheng-nan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 159-162. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.037

Abstract （325）

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The status of grassland rights determination in village B of Inner Mongolia was studied, and the principle of fairness affected the definition of property rights. The results show that the consistent principle of fairness can maintain the stability of local property rights. When the social structure changes, the interests of herdsmen are threatened, and the interpretation of fairness by different interest groups changes. The principle of fairness has changed from rules that restrict the behaviors of herdsmen into tools for herders to fight for their interests, and disputes arise. Government staff going to the countryside to participate in power determination is conducive to resolving conflicts.

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Livelihood capital and the transformation of farmers' livelihood strategies: Based on the survey of Zhejiang Kaihua National Park

ZHOU Ping, QI Di-ming

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 163-168. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.038

Abstract （353）

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The sustainable development of national parks requires attention to the sustainability of residents ’livelihoods. A sample survey was conducted on the residents of Gutian Mountain Natural Zone in Kaihua National Park, Zhejiang Province, and the livelihood strategies of farmers and their conversion effects were analyzed. The livelihood capital assessment system is used to measure the livelihood capital status of residents in protected areas, and logistics regression and multiple regression models are used to test the impact of livelihood capital on livelihood strategies and their transfer. The results show that the improvement of material and human capital will promote the conversion of pure agricultural farmers to non-agricultural and integrated agriculture, and natural capital will make them more inclined to integrated agriculture. The improvement of material, human, financial and natural capital will promote the diversification of farmers' income sources. The improvement of material, manpower, society, and nature will strengthen the career diversification of farmers' family members. Therefore, to transform the livelihood strategies of protected areas, it is necessary to increase investment in infrastructure, education and training, and ecological compensation.

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Evaluationg of regional comprehensive carrying capacity and obstacle diagnosis based on entropy method

WU Qing, YANG Jing, QIN Shuo-pu, LI Yu-ao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 169-172. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.039

Abstract （307）

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Taking Jilin province as an example,the regional comprehensive carrying capacity system is divided into four subsystems, such as economy, water and soil, society and ecology. Through correlation analysis and principal component analysis, the indexes were screened and the entropy method was used to objectively assign weights to construct the evaluation system of regional comprehensive carrying capacity. The degree of coupling coordination among the four subsystems was scientifically evaluated by using the coupling coordination degree model, and the main obstacle factors restricting the regional comprehensive carrying capacity were diagnosed. The results showed that the regional comprehensive carrying capacity and the degree of coordination between subsystems are overall upward trend, and part of the coupling coordination degree and the overall carrying capacity changes show the consistency. Water and soil carrying capacity has always been an important factor restricting the comprehensive carrying capacity, the early social constraints gradually transformed into ecological factors.The occurrence of natural disasters such as flood, has different degress of impacts on each subsystem and has a huge impact on the regional comprehensive carrying capacity. This study makes a scientific evaluation of the regional comprehensive carrying capacity and makes an obstacle diagnosis, providing a scientific basis for policy formulation and efficient development of Jilin province.

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Empirical on the impact of equity structure of agricultural listed companies on earnings management

SUN Jing-si

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 173-177. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.040

Abstract （351）

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Explore the relationship between the equity structure and earnings management of agricultural listed companies under their own unique industry environment, discover and participate in the constraints of earnings manipulation behavior in agricultural listed companies and help agricultural listed companies improve their equity structure to establish a more advanced and perfect corporate governance structure. The results show that,the equity concentration and earnings management have an inverted U-shaped relationship; the equity balance degree have a significant negative correlation with earnings management; the proportion of state-owned stock ownership and earnings management are significantly positirely correlated ; the management shareholding ratio and earnigs management are siginficantly negatively correlated; the institutional investors' shareholding ratio and earnings management are significantly negatively correlated; the equity liquidity and earnings management are significantly positively correlated.

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The research of the organic renovation of the Ya-que-lake community park based on SD method evaluation

LIANG Chun-fei, SU Feng-xiu, LIANG Xi-xian, LI Xi-cheng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 178-183. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.041

Abstract （373）

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Community park renovation as an important part of the urban park organic renovation, it is of great significance to use scientific method for evaluation before renovation. As a case of the Ya-que-lake community park in Changsha, the paper evaluates and analyzes the present situation of the park before renovation, using the SD method. And on this basis, the paper puts forward the suggestion of community-parks reconstruction with the organic renovation concept.

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Study on the temporal and spatial evolution and influencing factors of inbound tourism flow in Zhejiang province

LIU Shuang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 184-190. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.042

Abstract （395）

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Based on the data of the number of inbound tourists and foreign exchange income in Zhejiang province from 2000 to 2017, this paper uses quantitative methods such as ArcGIS and Matlab to study the evolution process of spatial and temporal pattern and influencing factors of inbound tourism flow in Zhejiang province. The study found that,the number of inbound tourists and foreign exchange income increased rapidly. From 2000 to 2017, the development trend of interannual change index of foreign tourists, Hong Kong tourists, and Taiwan tourists remained consistent. There is fierce competition among prefecture-level cities in Zhejiang province for inbound tourism flow,the flow of inbound tourism in Zhejiang province presents an increasing spatial and temporal distribution pattern from southwest to northeast. The flow quality of inbound tourism in Zhejiang province has been continuously improved, and the flow quality index of each prefecture-level city has changed greatly, and the spatial distribution difference has gradually narrowed. The tourism flow in Zhejiang province is greatly influenced by the GDP and the amout of foreign direct investment, while the tourism flow have great influence on the exchange rate change and the number of star-rated hotels.

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Practice and thinking of rural community management service under the strategy of rural rejuvenation

LUO Gang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 191-194. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.043

Abstract （441）

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Under the strategy of village rejuvenation, the rural community management service, as a rural management mode combining innovation and practicability, was the only way to promoted the role transformation of villagers. Restricted by external factors, the community management level in rural areas is difficult to be effectively improved in a short period of time, making it gradually fall into the embarrassing situation of public release, internal digestion and institutionalization dissipation.Only by building a two-way communication channel between "community and villagers", cultivating endogenous community organizations, and clarifying the rights and responsibilities of the management subjects, could we break through the practical difficulties and achieved the goal.

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Thinking on the legal issues of quality and safety of agricultural products under the background of “Internet + agriculture”

LIU Hui-ming, XU Wen-jie

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 195-198. DOI: 10.14088/jcnkiissn0439-8114.2020.10.044

Abstract （404）

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Internet technology has had a profound impact on the national economy and the rural economy. Through the use of the analysis ability of Internet big data, it has promoted the integration of the primary, secondary and tertiary industries in rural areas and promoted the transformation and upgrading of the rural economy. The frequent occurrence of agricultural product quality and safety problems also exposes the imperfections of agricultural product quality and safety laws. Therefore, on the premise of explaining the relevant legal concepts and analyzing the relevant legal defects, this paper proposed the legal regulation path of agricultural product quality and safety in the context of “Internet + agriculture”.

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Learning ability, socialization service and farmers' ecological agriculture technology adoption behavior:Taking rice and shrimp co-culture technology as an example

YANG Xing-jie, QI Zhen-hong, CHEN Xue-ting, HUANG Wei-hong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 199-205. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.045

Abstract （341）

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Based on a total of 612 microscopic survey data of farmers in Jiangsu province and Hubei province, the binary logistic model was used to empirically analyze the impact of farmers' learning ability on the adoption of rice and shrimp co-culture technology. On this basis, the regulatory role of agricultural socialization service was explored. The study found that farmers' ability to understand and communicate significantly positively affected their adoption of rice and shrimp co-culture technology, while their education level had no significant effect on their adoption of rice and shrimp co-culture technology. Government agricultural technology training only significantly positively regulates farmers' ability to understand and interact with rice and shrimp co-culture technology adoption behavior. Agricultural machinery employment service and transportation service have no significant role in regulating the learning ability of farmers and the adoption of rice and shrimp co-culture technology.

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Study on the cognition of organization department to ecotourism management

LIU, Feng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 206-207. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.046

Abstract （354）

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Aiming at studying the management indicators and strategies of sustainable ecotourism, this study explored the views of ecotourism managers on the management strategies of ecotourism and whether there were differences in the cognition of managers of different ecotourism sites on the management strategies. Based on the research results, this study proposed ecotourism management strategies.

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Research on automobile export trade potential and influencing factors between China and “the Belt and Road” countries

YANG Yan-ping

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 208-213. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.047

Abstract （389）

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Based on the stochastic frontier gravity model, China’s automobile export data from 66 countries of “the Belt and Road” from 2009 to 2017 are used to study China’s automobile export trade potential and influencing factors. The results show that the signing of free trade agreements, the liner shipping connectivity index of importing countries, the quality index of trade and related infrastructure, the political stability index, and the degree of freedom of trade are important factors that affect China’s automobile exports. The potential of automobile export trade is large, and the potential of automobile export trade to different countries is polarized.

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Study on agricultural economic evaluation of Henan province based on cluster analysis and principal component analysis

HU Jing-jun, XUE Meng-yao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (10): 214-217. DOI: 10.14088/j.cnki.issn0439-8114.2020.10.048

Abstract （379）

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Using cluster analysis and principal component analysis, 18 cities in Henan province were divided into 3 agricultural economic development categories according to 9 indicators, namely, leading agricultural economic development area, middle agricultural economic development area and relatively backward agricultural economic development area. The main indicator data of each category is analyzed, and the results show that there are large differences among regions. Three principal components that affect the level of agricultural economic development are selected, and the weights of various indicators are calculated to obtain the comprehensive scoring model for agricultural economic development. Finally, decisions and suggestions for various areas of agricultural economic development are targeted to promote Henan provide reference for the development of agricultural economy.

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Research progress of plant root expansion

WANG Liu-ping, QIN Kun-jian, ZHAO Li-chun, YAN Zhi-gang, HUANG Zhi-qi, TU Dong-ping

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 5-9. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.001

Abstract （477）

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The development and expansion of roots directly affect the yield of vegetables, grain and medicinal plants. Therefore, it is of great significance to understand the mechanism of root expansion for improving the yield and function of various crops mainly for harvesting roots.The environmental factors, the changes of assimilation products, the changes of endogenous hormones, and the effects of related genes in the process of root enlargement were discussed, which could provide reference for the study of the mechanism of root enlargement, especially for the study of the expansion of medicinal parts of root medicinal plants.

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Research progress of bean weevil resistance in mungbean

ZHANG Qin-xue, CHEN Jing-bin, YUAN Xing-xing, WU Ran-ran, YAN Qiang, ZHU Yue-lin, XUE Chen-chen, CHEN Xin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 10-13. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.002

Abstract （590）

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Mungbean （Vigna radiata L.） is an important legume crop in Asia, the germplasm resources are extremely rich and the nutritional value is high. However, its yield and quality are seriously affected by the bean weevil. Therefore, it is urgent to cultivate new varieties resistant to bean weevil. The research progress of mungbean at home and abroad was reviewed from the aspects of germplasm resources, mechanism of resistance to mung bean and genetic mechanism and location of resistance gene in order to supply the useful information to correlation studies.

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Adaptability evaluation of 35 flax varieties（lines） in Wuhan city

Rapkat PARHAT, ZHANG Zheng, PENG Ding-xiang, LIU Li-jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 14-18. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.003

Abstract （405）

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In order to enrich the winter flax（Linum usitatissimum L. ） varieties in the south, introduce and select the varieties suitable for local cultivation, the relational grade of original stem yield, plant height, process length, branch number, stem diameter, stem weight per plant, plant fruit number, 1 000-seed weight, grain weight per plant and other indicators of 35 flax materials were comprehensively analyzed by the grey relational analysis method. Results show that “651” has the largest correlation with the control variety 04076-4-9, which is suitable for planting in Wuhan city or similar ecological areas. M0459 and “609” ranked the second and third, showing certain development potential and suitable for promotion.

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Screen of aluminum toxicity tolerance in sweet corn inbred lines

LIN Jin-li, CHENG Chun-yuan, XIE Pei-feng, LIANG Ting-yu, LI Shi-yi, JIANG Feng, ZHANG Zi-li

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 19-24. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.004

Abstract （353）

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Aluminum toxicity is one of the major factors limiting crop production in acidic soils. Sweet corn （Zea mays L.） inbred lines breeding for aluminum toxicity tolerance is an effective way to solve this problem. To determine the identification method of aluminum toxicity tolerance and screen sweet corn lines with aluminum toxicity tolerance is of importance for aluminum toxicity tolerant breeding. 5 sweet corn lines with different growth vigor were selected to identify the aluminum toxicity tolerance by nutrient solution culture. The concentrations of aluminum ions were 0, 0.1, 0.2, 0.3 and 0.4 mmol/L, respectively. The fresh weight and dry weight of stems and leaf and root were measured on the 7th, 10th and 14th day after treated by aluminum toxicity. The results showed that the growth of stem, leaf and root of sweet corn slowed down with the increase of treatment concentration, and the results of aluminum resistance test on the 7th and 10th day of treatment were relatively consistent, in which, G107 and G139 showed the best tolerance to aluminum toxicity, G118 and G151 were in the middle level, and G79 showed the worst tolerance to aluminum toxicity. The activities of POD, CAT and SOD were detected at 14th day after treated by different concentration of aluminum ions, that the activity of three enzymes increased at low concentration, and then decreased with the increase of stress concentration.

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Methane production and oxidation potential of different types of soils in Caohai wetland lakeside zone

KOU Yong-zhen, LIN Tao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 25-30. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.005

Abstract （375）

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The methane production and oxidation potential of different types of soils in the lake-shore zone of Caohai wetland were studied by means of laboratory simulation culture technique. Results showed that the potential of methane production in Caohai wetland surface soil （0~5 cm） was the largest. The order of methane production potential was 10.448 μg/（g·d） of swamp meadow, 3.188 μg/（g·d） of meadow, 0.741 μg/（g·d） of primary swamp, 0.042 μg/（g·d） of cultivated land. The cumulative potential of methane oxidation was 0.155 7 μg/（g·d） of swamp meadow soil, 0.079 6 μg/（g·d） of meadow soil, 0.011 9 μg/（g·d） of cultivated land, 0.003 7 μg/（g·d） of primary swamp soil. With the increase of soil depth, the methane production potential of various types of land decreased obviously. The oxidation potential of all types of soils in Caohai wetland is lower than that in the production potential, which meaned, the soil in the lakeside soil of the Caohai plateau wetland did not have the potential to absorb methane from the atmosphere and reduce the methane in the atmosphere.

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Diurnal variation of N₂O fluxes in the typical marsh and shrub swamp in Xiaoxing'an Mountains

SHI Lan-ying, MU Chang-cheng, SUN Xiao-xin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 31-33. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.006

Abstract （342）

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In order to provide basic data for seasonal dynamics and total amount estimation of N₂O emission from mountain marshes, this study was performed to understand the diurnal variation of N₂O emission fluxes from Carex schmidtii marsh and Betula ovalifolia-Carex schmidtii shrub swamp in Xiaoxing'an Mountains. An experiment was conducted to observe the diurnal variation of N₂O fluxes in marsh and shrub swamp in Xiaoxing'an Mountains using the static opaque chamber and gas chromatography techniques on August 4-5 of 2007, September 24-25 of 2007, and June 4-5 of 2008. The results showed that the diurnal variation of N₂O emission flux in two kinds of marshes showed a diurnal high-night low pattern on August 4-5 of 2007, the daily emission regularity is not significant on September 24-25 of 2007 and June 4-5 of 2008. The highest N₂O fluxes in marsh were appeared at 9：00 on August 4-5 of 2007 and June 4-5 of 2008, and appeared at 3：00 on September 24-25 of 2007. The lowest N₂O fluxes in marsh were appeared at 21：00 on June 4-5 of 2008 and September 24-25 of 2007, and appeared at 18：00 on August 4-5 of 2007. The highest N₂O fluxes in shrub swamp were appeared at 15：00 on August 4-5 of 2007 and appeared at 6：00 on September 24-25 of 2007, and appeared at 9：00 on June 4-5 of 2008. The lowest N₂O fluxes in shrub swamp were appeared at 21：00 on August 4-5 of 2007, and appeared at18：00 on September 24-25 of 2007 and appeared at 3：00 on June 4-5 of 2008. Two kinds of marshes were the absorption sinks of N₂O on June 4-5 of 2008, and were the weak emission sources on August4 -5 and September 24 to 25, 2007. The diurnal variation N₂O fluxes of shrub swamp were higher than those of marsh. The daily N₂O emission flux of two kinds of marshes were negatively correlated with temperature on September 24-25 of 2007 and June 4-5 of 2008, and were Positive correlation with temperature on August 4-5 of 2007. Among them, the daily N₂O emission fluxes of marsh were negatively correlated with 10 cm soil temperature, while that of shrub swamp were negatively correlated with 15 cm soil temperature. The diurnal N₂O emission fluxes of marsh were positively correlated with surface temperature, while that of shrub swamp were positively correlated with 40 cm soil temperature. The marsh and shrub swamp in Xiaoxing'an Mountains were weak sources or sinks of N₂O emission, the temperature, water level and vegetation types were the main influencing factors of N₂O daily emission fluxes.

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Effects of different application methods of basic fertilizer on yield and quality of flue-cured tobacco

LIU Run-sheng, XU Wei, PENG Yao-dong, XIAO Xian-yi, ZENG Hao, ZENG Zu-bing, CHEN Guo-jin, OUYANG Wei-wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 34-38. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.007

Abstract （440）

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To study the effects of different application methods of basic fertilizer on the yield and quality of flue-cured tobacco, four different basic fertilizer application methods, namely single furrow strip application, wide strip application, hole bottom ring application and “101” fertilizer application method, were adopted to carry out field randomized block experiment under the condition of equal amount of fertilizers . The labor cost of different fertilizer application methods for Yunyan87 varieties, field growth status of tobacco leaves, appearance quality of cured tobacco leaves, coordination of chemical components and economic characteristics were studied. The results show that broad band application of basic fertilizer is beneficial to the early growth and rapid development of tobacco plants, can improve the yield and quality of tobacco leaves, improve the coordination of chemical components of tobacco leaves and has low fertilization cost, so it is worthy of comprehensive promotion and application. Base fertilizer hole bottom ring application method and “101” fertilizer application method are beneficial to increase the average price of tobacco leaves, the proportion of superior tobacco leaves and improve the quality of tobacco leaves, but are not conducive to early growth and rapid development, low yield, high fertilizer application cost and difficult to popularize. The method of applying basic fertilizer in single furrow strip has high yield and output value and low fertilizer application cost, but the average price is low and it is not conducive to improving the quality of tobacco leaves and the coordination of chemical components is poor.

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Effect of fertilizing levels on photosynthesis, yield and quality of Junzhi jujube under drip irrigation condition

WANG Jing-jing, CHEN Qi-ling, LI Ming, ZHENG Qiang-qing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 39-42. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.008

Abstract （299）

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Taking 6-year-old jujube（Zizyphus jujuba）as test material, the effects of different fertilization amount on photosynthesis, yield and quality of Junzhi jujube were studied under drip irrigation condition, the correlation between nitrogen, phosphorus and potassium elements and yield was studied, each fertilization treatment was comprehensively evaluated by the membership function method.The results show that potassium fertilizer is the most beneficial to the photosynthetic potential of jujube leaves, follow by nitrogen fertilizer and phosphate fertilizer, and it is more beneficial to the effect of potash fertilizer when nitrogen fertilizer is sufficient; It can increase yield, fruit vertical and horizontal diameter, fruit weight and commodity fruit rate when nitrogen and potassium fertilizers are sufficient, phosphorus fertilizer is appropriate; The best fertilization formula of N、P₂O₅、K₂O of Junzhi jujube with drip irrigation in Nanjiang is 1∶0.66∶0.83.

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Environmental assessment of GAP cultivation of Nauclea officinalis

KE Ze-tao, LI Jin-yun, ZHANG Peng, LIAO Jin-hong, LI Yong-hui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 43-46. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.009

Abstract （425）

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To evaluate the environmental quality for the planting of Nauclea officinalis, and provide scientific basis for planting specifications of Nauclea officinalis, and then establish GAP planting practice of Nauclea officinalis, the environment factor of main planting areas of Nauclea officinalis, including soil, surface water and atmosphere, were comprehensively analyzed and evaluated. The air environment, water resources and soil of Xinshi farm planning area, Qiongzhong county all meet the GAP standards. The environmental quality of GAP planting area of Nauclea officinalis fit the environmental quality requirement of good medicinal material planting.

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Studies on physiological effect of sodium selenate stress in Cardamine hupingshanensis

LUO Jin-ling, YANG Guang, CHEN Hong-hui, ZENG Wei-chao, WANG Li, CHEN Da-qing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 47-50. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.010

Abstract （417）

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Cardamine hupingshanensis seeds was cultivated in MS culture medium with different sodium selenate concentration （0, 20, 40, 60, 80, 100, 200, 300 mg/L）. The physiological and biochemical indexes such as seed germination rate, seedling survival rate, yellowing rate, plant height, root length, fresh weight, dry weight, POD activity and CAT activity were measured to discuss the response of selenate stress in pots to Cardamine hupingshanensis. The results showed that, the low concentration of sodium selenate was used to promote the growth and biomass of the Cardamine hupingshanensis, on the contrary, it would inhibit it. When the concentration of sodium selenate was 40 mg/L, Cardamine hupingshanensis had the highest germination rate and seedling rate, at the same time, the root length, plant height, fresh weight and dry weight of the seedlings reached the maximum value. The yellowing rate of Cardamine hupingshanensis seedlings increased with the increase of selenium salt concentration. The POD activity of the Cardamine hupingshanensis was decreased first and then increased, the lowest value reached at 40mg/L sodium selenate solution. The CAT activity of the Cardamine hupingshanensis was increased first and then decreased, the highest value reached at 100 mg/L sodium selenate solution.

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Analysis on physical and chemical properties of soil under different sowing date of rice in central Guizhou province

ZHANG Hao, YUAN Shuo, ZHANG Xue-bin, JIN Zhao-gui, WENG Ling, WANG Lu-jing, YANG Tao, TENG Wan-li, LI Juan, XU Reng-xiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 51-56. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.011

Abstract （404）

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Soil of four stages of rice （Oryza sativa L. ） transplanting were used as research objects to explore the differences of physical and chemical properties of rice soil at different sowing date. Daily data of air temperature and mud temperature were collected, and soil pH, nutrient content, mechanical composition and exchangeable base cations were measured. Through variance analysis and correlation analysis, the differences of physical and chemical properties of rice soil at different sowing date were discussed, and the soil comprehensive scores at different sowing date were calculated by principal component analysis. The results showed that soil nutrient content was from suitable to rich. The proportion of Ca²⁺ in the exchangeable base components was the highest, and the soil composition was mainly composed of silty grains. The content of clay grains of rice soil transplanted May 9 was the highest, and the corresponding fractal dimension was the largest. The results of correlation analysis showed that the correlation coefficient between K⁺ and Mg²⁺was 0.953; Organic matter, total nitrogen and Na⁺ showed significant positive correlation; pH was significantly correlated with average temperature, while available potassium was significantly positively correlated with accumulated temperature; The clay content showed strong positive correlation with most indexes. Principal component analysis of various factors for different sowing date showed that the suitable date for rice transplanting was May 9.

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Analysis of soil reducing substance content, economic effect in different sowing times of integrated rice-crayfish system

TIAN Yu-cong, FENG Xiang-zhao, GAO Zhen-zhen, DUAN Men-jun, ZHU Jie , LIU Zhang-yong, CHEN Fu, JIN Tao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 57-61. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.012

Abstract （421）

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The different sowing date collocation with rice varieties of different growth stages was set to change the duration of soil flooding before planting rice.The soil reductive state of paddy field tillage was investigated, and the economic benefit of integrated rice-crayfish system was evaluated using the input-output method. The results showed that in integrated rice-crayfish system, changing rice sowing time, the total amount of soil reductive substances in paddy field did not change significantly, but the content of soil active reductive substances, active organic reductive substances and Fe²⁺ all increased with the flooding time prolonged, especially the content of Fe²⁺ increased significantly. Rice cultivation could significantly improve the soil reductive state of the tillage layer and reduced the soil Fe²⁺ content. After rice harvesting, the Fe²⁺ content of the three models tended to be the same, with no significant difference among them.With the delay of sowing time, the output value of rice gradually decreased, the cost of integrated rice-crayfish system increased, but the output value of crayfish increased significantly, and the comprehensive output value of integrated rice-crayfish system increased. However, with the delay of sowing time, there was less room for increase in the comprehensive output value of integrated rice-crayfish system.

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Study on enhance treatment of marine aquaculture wastewater by immobilized bio-diatomite in CAST reactor

CHEN Jin-bin, CAO Jun-rui, MIAO Ying-xia, XIE Bao-long, MA Xiao-lei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 62-64. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.013

Abstract （303）

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The three aerobic denitrifier isolated from sediment of one marine aquaculture farm in Tianjin city were made into aerobic denitrification microbial agent in a ratio of 3∶2∶1, and 5% polyvinyl alcohol, 2% sodium alginate and 3% diatomite was used to embed the aerobic denitrification microbial agent to form immobilized bio-diatomite. The immobilized bio-diatomite was added to the cyclic activated sludge technology （CAST） reactor to investigate the treatment capability of COD, inorganic nitrogen and phosphate in marine aquaculture wastewater by the cyclic activated sludge technology reactor with immobilized bio-diatomite. The results showed that under the same operation condition, the reduction rate of COD, inorganic nitrogen and phosphate by the cyclic activated sludge technology reactor with immobilized bio-diatomite can keep above 83%, 99% and 96%, respectively. It was 30 pecentage points, 28 pecentage points and 55 pecentage points higher than that of the traditional cyclic activated sludge technology reactor, respectively.

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Characteristics of sunshine variation and its impact analysis in eastern Gansu province

REN Ji-bang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 65-68. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.014

Abstract （462）

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To provide theoretical basis for making full use of sunshine resources to develop local ecological agriculture, develop and utilize solar energy, and construct livable urban in eastern Gansu province, the meteorological data of representative area including Xifeng district, Kongtong district and Huanxian county was used to analyze the interannual variation characteristics of sunshine hours and tested the sudden change of sunshine hours by Mann-Kendall method. The results showed that the change of sunshine hours in eastern Gansu province showd a decreasing trend, the reduction rate was followed as orders of Kongtong district, Xifeng district and Huanxiancounty from big to small, and the sunshine hours in Kongtong decreased most obviously, but the reduction rate of sunshine hours was less than that of the whole country. The annual variation of sunshine hours in different regions had its own characteristics. In the past ten years, the annual sunshine hours were on the low side, and in summer, autumn and winter, they were also on the low side. The sudden change periods of sunshine hours were different, there were two obvious mutations in Kongtong district, with the earliest mutation time, and the latest mutation time of Xifeng district. On the whole, the sunshine resources in eastern Gansu province are relatively abundant, which is conducive to the develope and utilize of solar energy, improve crop photosynthesis efficiency, develope characteristic agriculture and construct ecological environment. In the planning of agricultural industry development, we should take measures according to local conditions, combine with the light characteristics of crop growth, introducing crops appropriately, arranging reasonably, making full use of light energy efficiency, to vigorously develop facility agriculture and characteristic agriculture, and improve crop quality and yield.

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Study on urban thermal environment effect of Changsha based on Landsat-8 OLI/TIRS image

HE Bing-wei, HAN Qin-zhe

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 69-75. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.015

Abstract （363）

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Based on Landsat 8 OLI/TIRS data of Changsha on July 23^th of 2016, the surface temperature was retrieved by using Qin Zhihao single window algorithm. The spatial distribution characteristics of urban thermal environment and the influence of main surface ecological environment parameters on urban thermal environment effect were analyzed. The results show are as follows（1） The surface temperature of the main urban area of Changsha is relatively higher, and the high temperature areas are mainly distributed in areas with high density urban construction, single function of urban construction and less vegetation coverage; （2） the range of heat island area of Changsha is basically the same as the built-up area of the city, the super-high temperature area and the high temperature area which constitute the heat island area are mainly distributed in the Central District and Changsha County.Overall, the Urban-Heat-Island-Ratio-Index is as high as 0.712, and the urban heat environment situation of Changsha has become more severe; （3） Quantitative analysis of the relationship between surface parameters and surface temperature shows that the built-up land plays a role in increasing surface temperature, while vegetation does the opposite, and the heating effect of the built-up land is much stronger than that of vegetation. Therefore, in the future urban planning, the urban heat island effect can be effectively alleviated by increasing vegetation while reducing the area of construction land.

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Edible value investigation of medicinal plants in western Hubei province

XIAO Bing, YU Jie, LI Xue-yin, MA Yue-ling, LI Shao-ting, XU Ran

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 76-80. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.016

Abstract （368）

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The medicinal plants with edible value in western Hubei province were investigated by means of thesis search, book checking and field investigation, and were catalogued and summarized according to the alphabetical order of Latin name of the medicinal plants. The population of medicinal plants contained a total of 54 species, and theirs basic source, edible parts, edible methods, functions, related matters needing attention and standard record were introduced mainly, so as to provide references for their further utilization, developement and promotion.

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Study on rooting condition optimization of tissue culture seedling of Chinese fir

JIANG Fan, MAI Kai-le

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 81-83. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.017

Abstract （354）

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The rooting quality of Chinese fir [Cunninghamia lanceolata （Lamb. ） Hook. ] tissue culture seedlings was optimized from two aspectsFirst, high concentration and low concentration hormone medium were used to optimize the quality of tissue culture seedlings at the stage of proliferation; Second, the rooting quality of Chinese fir tissue culture seedlings was optimized by changing the light source during rooting stage. The results show that the proliferative buds treated with high concentration hormone and low concentration hormone has high proliferation rate, fast growth, moderate knot spacing and high lignification degree et al. With the increase of subculturing times, these characters are relatively stable. The rooting rate reached 86.5% and the rooting number reached 2.7 after the proliferative buds were planted to the rooting medium. The rooting rate reached 86.7% when the red-blue light ratio was 2∶1; The number of rooting reached 2.8 when the red-blue light ratio was 4∶1.

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Effects of different shading treatments on photosynthetic characteristics, yield and quality of strawberry

YUE Gao-feng, HAN Zhi-qiang, XUE Zhi-wei, LIU Hui, YANG Wen-yue

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 84-88. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.018

Abstract （466）

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The growth, photosynthetic characteristics, yield and quality of strawberries （Fragaria ananassa Duchesne） were studied under different shading conditions of 0 （CK）, 30%, 50% and 70%. The results showed that different shading degrees had obvious effects on the photosynthetic characteristics and yield and quality of strawberry. Under 30% shading degree, the photosynthetic characteristics and yield and quality of strawberry were the best. Under the condition of 0 （CK） and 30% shade, the diurnal curve of strawberry photosynthesis was bimodal, showing the phenomenon of “noon break”, while under 50% and 70% shade, it was single peak. The net photosynthetic rate, transpiration rate, stomatal conductance and intercellular CO₂ concentration of strawberry decreased with the increase of shading. The light saturation point, light compensation point and dark respiration of strawberry decreased with the increase of shade. Strawberry has a strong ability to utilize light energy in low light environment.

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Screening of cold resistance rose varieties by utilization of branches germination rate

ZHUANG De-feng, MA Ce, XU Ning, LI Zhen, WANG Cheng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 89-92. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.019

Abstract （395）

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Twenty rose varieties were collected to analyze cold resistance according to germination test by using cutting branches, combined with survival rate under natural conditions with temperature change. The results indicated that five varieties of Love, Eiffel Tower, Fen Shan, Paradise, New Spartan were entirely frozen to death, which were not suitable for landscaping application in north area or Liaoyang, Liaoning province. Otherwise, Hadefujun, Chivalry, Orange Fair, Tianshan Xiangyun, Utano were survived under -25.2 ℃ in Liaoyang. Angela, Viva, Cardinal Hume, Queen Elizabeth were frozen in overground part, but still can survive because of not frozen in underground part. The rate of water loss at low temperature can be used as a macro index to evaluate the cold resistance in rose. In the study, nine rose varieties were selected with a certain cold-resistance, which can be provided for foundations on city landscape of north area in the future.

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Analysis on chemical component and sensory quality of flue-cured tobacco in Huidong county

SHI Gang, ZHAO Xiao-chao, PAN Hao-dong, ZHANG Zhen, FU Long, WANG Bao-xiang, WANG Jian, LING Ai-fen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 93-98. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.020

Abstract （383）

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Variability of chemical component contents and sensory quality between 2014 and 2018 was statistically analyzed by 350 of flue-cured tobacco samples with grades of B2F and C3F from Huidong county. The results showed that, in average, chemical components of flue-cured tobacco were almost in normal range of high quality flue-cured tobacco, the contents of total sugar and reducing sugar were higher than the average. The variation coefficients of chlorine content and potassium chlorine ratio were the largest, while the variation coefficients of total sugar, reducing sugar and total nitrogen were smaller. There existed obvious variation among chemical components of different grades in different years. The variation coefficients of aroma quality and irritability of flue-cured tobacco in Huidong county were large. The variation coefficients of softness, delicacy, roundness and dryness are low. Smoking sensation of flue-cured tobacco is stable. The variation coefficients of sensory quality of flue-cured tobacco B2F and C3F were larger in 2014 and smaller in 2018.

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Effect of baking upper leaves with stem on flue-cured tobacco upper leaf quality in different harvest maturity

HE Jie-wang, LI Fang-ming, PENG Wu-xing, DONG Shi-liang, SI Guo-han, PENG Cheng-lin, LIU Jian-feng, ZHOU Jian-xiong, XIE Yuan-yuan, ZHAO Shu-jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 99-103. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.021

Abstract （486）

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The experiment was designed to study the effect of baking tobacco leaves with stem on leaf quality and economic indexes of indifferent maturity tobaccos leaves. Results showed that, compared with conventional baking treatment, the rate of first-class tobacco leaves baked with stem, from fully mature, part mature and immature tobaccos leaves treatment, increased by 6.0, 8.7 and 16.9 percentage points, respectively. Weight of single baked leaf decreased by 0.6~1.1 g, and the baked fully mature leaves decreased less than the baked part mature and immature tobaccos leaves. Thickness of single baked leaf decreased by 0.04~0.08 mm compared with conventional baking treatment. The content of reducing sugar in baked tobacco leaves from fully mature and immature tobaccos leaves treatment increased 2.38 and 2.33 percentage points, respectively, whereas that from part mature tobaccos leaves treatment decreased 1.48 percentage points compared with conventional baking treatment. Additionally, tobacco appearance and sensory quality were improved, the quality, quantity and concentration of tobacco aroma were all increased. The study indicated that, compared with conventional baking, baking tobacco leaves with stem improved the appearance and internal quality, as well as economic profit of insufficiently mature tobacco leaves, and therefore increased the usage of upper tobacco leaves.

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Antibody levels of H5, H7 and H9 subtypes of avian influenza virus of broilers and layers

LI Jia-kai, GUO Kang-kang, LIU Li-na, WEN Guo-yuan, LUO Qing-ping, LI Zi-li, DONG Shi-shan, SHAO Hua-bin, ZHAO Zong-zheng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 104-106. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.022

Abstract （541）

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In order to understand the immune antibody level of H5, H7 and H9 subtypes avian influenza virus in poultry, the blood samples of four experimental observation stations in Neijiang city of Sichuan province, Jinan city of Shandong province, Hebi city of Henan province and Yangzhou city of Jiangsu province were analyzed by serological investigation and hemagglutination inhibition test（HI）, including two broiler farms and three layer farms. The research found that layers were immunized with H5, H7, H9（or H5 and H9） subtypes avian influenza vaccines, while broilers were only immunized with H9 subtype avian influenza vaccine. The layer farms and broilers farms had low antibody production after the immunization of avian influenza vaccine, leading to unqualified or immune failure. It is suggested that the immunization different subtypes of avian influenza vaccine and the monitoring of avian influenza antibody levels after immunization should be strengthened to prevent the occurence and prevalence of avian influenza.

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Study on coupling expansion of different form tobacco stem

CHEN Wan, LI Jin, LIU Xin, LUO Xiao-ge, DU Chao, XIAO Yong-yin, XU Shi-tao, GAO Ze-rui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 107-111. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.023

Abstract （345）

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In order to study the difference of different forms of tobacco stem after microwave coupling expansion, the long stem was divided into thick long stem and fine short stem. The optimum coupling expansion conditions were explored from three aspectsraw material moisture, expansion time and microwave power. Then the coupling expansion was carried out under the optimum technological conditions, and the relative contents of conventional chemical components and aroma components were determined respectively. The results show that, ①with the increase of raw material moisture, the expansion degree of coarse and short pedicel increased at first and then decreased, and tended to be the highest when the moisture content of raw material was 12%~14%. ②with the increase of expansion time, the expansion degree and color difference of coarse and short pedicels increased. After the expansion time exceeded 2 min, the expansion degree of coarse and long pedicels exceeded that of slender pedicels, and the difference became larger and larger. During the expansion time, the color difference of thin and short pedicels was higher than that of coarse and long pedicels. ③The best value of the microwave power of the coarse and short stems is about 700 and 600 W, respectively; ④The contents of total nitrogen, chlorine and potassium changed little after coupling expansion, but the contents of total sugar and reducing sugar decreased in varying degrees. The changes of total plant alkaloids before and after expansion were opposite; ⑤The change trend of alcohols, heterocycles, phenols, ketones, coarse and short pedicels was the same, while that of some acids, lipids, alkenes and alkenes was opposite after the coupling expansion of coarse and short pedicels.

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Analysis of volatile components in different dosage forms of Chuanxiong Chatiao preparations by HS-SPME-GC-MS

WANG Wen-tong, JIANG Han-mei, GAN Jian-feng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 112-119. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.024

Abstract （396）

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HS-SPME-GC-MS was used for analysis the volatile components of Chuanxiong Chatiao preparations in different dosage forms. The results showed that 16 components were identified in the 24 peaks separated by Chuanxiong Chatiao granule, which accounted for 89.13% of the total volatile components. 46 components were identified in the 63 peaks separated by Chuanxiong Chatiao tablet, which accounted for 80.91% of the total volatile components. 69 components were identified in the 94 peaks separated by Chuanxiong Chatiao pill （concentrated pills）, which accounted for 90.10% of the total volatile components. 68 components were identified in the 101 peaks separated by Chuanxiong Chatiao pill （water-bindered pills）, which accounted for 78.50% of the total volatile components. The volatile components in different dosage forms of Chuanxiong Chatiao Preparations were different in chemical compositions and content.

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Determination of tetramine residues in food poisoning by GC-MS/MS spectrometry and evaluation of uncertainty

PANG Xiao-lian, LI Qiang, CHEN Yu, TANG Ming-hua, LU Sen-hua

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 120-124. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.025

Abstract （350）

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To establish a GC-MS method and a scientific uncertainty evaluation model for the determination of tetramine residues in food poisoning, and to improve the quality control level of detection. The results showed that the linear relationship between the concentration of tetramine 25.0~250.0 ng/mL was good, Y=1.181×10³X+5.203×10³（R²=0.995 3）. The average recovery of three levels was 96.57%, and the corresponding RSD was 2.7%. The detection limit of the method was 0.098 μg/kg. When the content of tetramine in food poisoning was 0.186 7 mg/kg, the expanded uncertainty was 0.000 21 mg/kg. The method of determination of tetramine residues in food poisoning was confirmed, and the reference basis was provided for the evaluation of uncertainty in the determination of tetramine by GC-MS/MS.

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Determination of hyperuricemia drugs for treatment illegally added in Chinese patent medicines and health foods by UPLC-MS/MS

CHEN Yu, LU Sen-hua, PANG Xiao-lian, XU Meng, QIU Yi-xian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 125-130. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.026

Abstract （347）

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To establish a method for simultaneous detection of 7 hyperuricemia drugs for treatment illegally added to Chinese patent medicines and health foods by UPLC-MS/MS．The samples were extracted from samples with mobile phase, the column was a ACQUITY UPLC® BEH C18（2.1 mm×100 mm, 1.7 μm）, a gradient elution with the mobile phase of 0.2% formic acid aqueous solution and acetonitrile solution at a flow rate of 0.3 mL/min. The compounds were detected by electrospray ion source in positive mode with multiple reaction monitoring （MRM） mode．The results showed that all the 7 hyperuricemia drugs for treatment with showed good linearity in their reasonable ranges , and the correlation coefficients were greater than 0.995.The average recoveries of low, medium and high concentrations ranged between 95.49% and 104.87%, and the relative standard deviations were 1.40%~3.44%. The LODs were 0.01~0.30 μg/kg and LOQs were 0.04~1.00 μg/kg.Illegal added chemical drugs were found in 1 of the 6 tested practical samples. With the advantages of simple pre-processed, high sensitivity and accurate results, the method can be used in determination of 7 hyperuricemia drugs for treatment in Chinese patent medicine and health foods.

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Cloning and sequences analysis of matK gene of Amorphophallus konjac from Ankang city

ZHOU Xiao-fan, LI Jing, WANG Rui, LI Rui, WANG Rui-xian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 131-133. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.027

Abstract （380）

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The genome of the Amorphophallus konjac from Ankang city was extracted and the matK gene sequence was amplified. After confirmed by gel electrophoresis, amplified product was sequenced and analyzed. Results showed that the matK gene of Amorphophallus konjac from Ankang city was 1 551 bp in length and predicted to encode 516 amino acids. The phylogenetic tree showed that the Amorphophallus konjac had the closest relationship with Amorphophallus maxwellii, Amorphophallus corrugatus and Amorphophallus krausei.

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Bioinformatics analysis of AkMYB5gene from Actinidia kolomikta （Maxim. et Rupr.）, Maxim

LIU Dan, LI Ran-hong, CHEN Xin, WANG Li-feng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 134-137. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.028

Abstract （475）

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To obtain information about AkMYB5 gene from Actinidia kolomikta （Maxim. et Rupr.） Maxim., the bioinformatics methods and tools were used to analysis the physical and chemical properties, hydrophilicity/hydrophobicity, transmenbranne domain, structure and function domain , secondary structure, tertiary structure, protein modification sites and homology of MYB5 from Actinidia kolomikta. The results showed that the length of AkMYB5 cDNA was 1 339 bp and the coding area was 1 053 bp coding 351 amino acid. AkMYB5 was a unstable and hydrophilic protein belonging to the MYB5 superfamily （PLN03212）. The analysis also demonstrated that AkMYB5 main component was Alpha-helix and random coil. Compaired to MYBs of the other 18 plants, AkMYB5 had near relationships with Actinidia chinensis var. chinensis.

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Research on benefit coordination mechanism of laying hens supply chain based on revenue sharing contract

HUANG Ming-tian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 138-141. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.029

Abstract （374）

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The problem of uneven benefit distribution is common in the laying hens supply chain. A fair and reasonable benefit coordination mechanism is the basis for the sustainable existence and stable development of the laying hens supply chain alliance. The revenue sharing contract is introduced to establish the coordination mechanism of the laying hens supply chain under the mode of “company + farmer”, and the value range of its coefficient is calculated. The results show that the revenue sharing contract can achieve the coordination of the laying hens supply chain and promote the fair distribution of the whole profit.

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Statistical method of farm profit and economic risk based on Copula-CVaR

DANG Rong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 142-145. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.030

Abstract （314）

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A statistical model for calculating farm profit and economic risk was presented in view of the influence of various factors on farm crop yield and agricultural income. On the basis of the existing statistical theory, the model used the conditional value-at-risk （CVaR） to evaluate the effectiveness of geographical diversification. CVaR was used to benchmark losses, while the Copula function was used to model the joint distribution of marginal revenues. The results showed that geographic diversification could be a feasible agricultural risk management method for wheat farm portfolio managers, which could achieve the optimal expected returns while controlling the risk. Compared with the traditional multivariate normal distribution model, the average CVaR model based on Copula could better simulate extreme losses, providing an innovative solution for agricultural risk management.

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The spatio-temporal variation of tourism economy in Shanxi province

LU Xin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 146-152. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.031

Abstract （352）

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A new tourism development planning for three plates of Yellow River, Great Wall and Taihang Mountain was made by the government of Shanxi province in the background of economic restructuring and the integration of culture and tourism. It means that the tourism economy of Shanxi has entered a new stage.The spatio-temporal variation of tourism economy in Shanxi province diminished gradually, by observing some indicators, such as Gini coefficient, absolute deviation, relative deviation, Theil index and so on. On the basis of correlation analysis of tourism income and influencing factors, economic development level, tourism resource, tourism infrastructure and services, the government of Shanxi province should reduce the regional difference of tourism economy by obeying the concept of comprehensive tourism, fulfilling the new tourism development planning for three plates, and developing cultural tourism resources.

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Research on financial risk evaluation of software and information technology service enterprises： Based on data from the GEM market in the eastern region

PENG You-yuan, WEI Cong-cong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 153-158. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.032

Abstract （444）

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Taking 35 listed companies in the GEM software and information technology service industry in the eastern region as samples, the key financial indicators of the sample companies in 2018 were analyzed by use of factor analysis, identifying the main factors affecting the financial risk of listed companies in the software and information technology service industry, Building a financial risk evaluation model for listed companies, scoreing and ranking the degree of financial risk. Based on the comprehensive score, the cluster analysis method was used to divide the company‘s financial risk into four categoriesSafety, low risk, general and high risk. The results showed that there are three main reasons for the company’s high financial risk. ①Some companies ‘net profit has fallen; ②Enterprises’ solvency was generally weak; ③Some high-risk enterprise products were in the initial stage and face greater financial pressure.

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Analysis of risk preference, interpersonal relationship and chemical application behavior of rural households under aging

XU Sheng-xiang, DONG Zhen-wu, HE Juan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 159-169. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.033

Abstract （394）

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Based on 441 micro survey samples of farmers, this study analysised the impact of aging rural labor force on chemical input from the aspects of various fertilizer use, pesticide use and purchase cost, and explored the impact mechanism of aging rural labor force from the aspects of physical condition, sense of control, skill demand, risk attitude and interpersonal relationship, so as to further investigate the individual characteristics of rural elderly labor force the heterogeneity effect on chemical input. The results showed that the aging of rural labor force would significantly reduce the application amount of compound fertilizer and the purchase cost of chemical fertilizer. The aging still had a significant negative impact on chemical input after controlling the variables such as physical strength and skill demand, which indicated that the physical strength and technology adoption speculated in previous studies were the ways for aging to affect farming, and the impact mechanism could not be fully revealed. Further research shows that aging of rural labor will affect the interpersonal relationship of farmers and the risk preference on farming input marginal effect. The conclusion shows that the policy of building ecological and livable beautiful countryside in China can promote the national food security strategy.

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The packaging design and brand building of the Bama tea in e-commerce environment

DONG Xian-juan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 170-174. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.034

Abstract （513）

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To explore how does Bama tea industry take the initiative to adapt the new environment and constantly explore the improvement of tea packaging design, and how to construct their brand through the corporate visual identity design and packaging design, when the marketing of e-commerce has become an important channel of the present tea sales. This research is starting from the change of tea sales mode in the e-commerce environment, pointing out that the logo design and packaging design of tea enterprises in the e-commerce environment are the most important channels for consumers to understand the products. Meanwhile, the paper analyzes the characteristics of packaging design of Bama tea, through the aspects of material, color, pattern and word. Then the paper analyzes how Bama tea builds the brand from the logo design and packaging design. The research shows that the packaging design of Bama tea has exquisite materials and technology, applys chinese traditional pattern, colors and word, which is seem both have historical and modern simply style and identifiability. Through the corporate visual identity system and unique packaging design, Bama tea industry has created a unique “Bama” brand image.

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Study on poverty alleviation efficiency of agricultural insurance in China under the support of “great poverty alleviation”policy

MENG Qing-jun, MIAO Ji-xiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 175-179. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.035

Abstract （314）

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Under the background of creating the pattern of “great poverty alleviation” and further promoting the implementation of poverty alleviation work, we should give full play to the role of agricultural insurance in poverty alleviation. This study choosed the time series data from 2009 to 2018, and used the time series DEA model to evaluate the efficiency of poverty alleviation in agricultural insurance in China. The results showed that the efficiency of poverty alleviation of agricultural insurance in China was generally good, but it was not effective year by year, and there was a problem of investment redundancy in the premium income of agricultural insurance, that was, the capital allocation was unreasonable. Finally, the paper puts forward some policy suggestions to promote the poverty alleviation of agricultural insurance.

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Analysis of spatial distribution pattern of leisure agriculture in Hubei province and its influencing factors

LI Fu-lin, TANG Jia-yao, LI Jiang-feng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 180-185. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.036

Abstract （465）

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Taking the 165 leisure agriculture demonstration sites of Hubei province published in 2010—2017 as the research object, the distribution type, distribution density, distribution clustering and spatial autocorrelation of the demonstration sites of leisure agriculture in Hubei province are described by using the methods of nearest neighbor index analysis, nuclear density analysis, three-step iteration method of Voronoi diagram, spatial autocorrelation analysis, buffer zone analysis, and two-sided correlation test; and many factors that may affect the development of leisure agriculture in Hubei province are analyzed and discussed. The results show that the distribution of leisure agriculture demonstration sites in Hubei province shows a certain degree of aggregation, and the East Hubei is the most densely distributed. The distribution of leisure agriculture is obviously affected by factors such as altitude, traffic location, and market environment, and the local economy and population density gradually become secondary.

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Research on rural water environment governance：Based on Olson's collective action theory

LIU Yi-nan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 186-190. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.037

Abstract （383）

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Using Olson’s collective action theory, China’s rural water environment governance issues were analyzed. The results show that rural water environment governance, as a public good, requires the participation of villagers, enterprises and local governments. However, in order to realize their own interests, all the main bodies have appeared to “free ride”. To achieve effective governance of the rural water environment, it is necessary to establish a perfect public participation incentive mechanism, enterprise incentive mechanism and local government incentive mechanism to overcome the “free rider” behavior of each governance subject, enhance the subject ’s awareness and action ability to participate in rural water pollution governance and realize sustainable development of rural water environment.

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Research on evaluation indicator system of water environment governance performance：Based on balanced score card

MA Ye-wen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 191-194. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.038

Abstract （342）

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The status of the water environment of the Huai River Basin and the benefit value of the governance performance evaluation were analyzed. Taking the Huai River Basin Water Resources Protection Bureau as the research object, using the balanced scorecard as the performance evaluation method, the indicator system is constructed from four dimensionsfinancial indicators, customer indicators, internal process indicators, learning and growth indicators. the scientific evaluation of the governance input of the Huai River Basin Water Resources Protection Bureau was carried out. So as to discuss the rationality of relevant water environmental protection policy formulation and expand new ideas for water environment governance.

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Visual analysis of GIS research hotspots in ecological environment：Based on method of mapping knowledge domain

XIE Lian-ke, ZHANG Yong, ZANG Yu-wei, MA Xin-gang, ZHANG Guo-ying, LIU Hui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 195-202. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.039

Abstract （379）

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Taking GIS and ecological environment field as the theme, the 1999—2018 periodical papers in the CNKI database are retrieved as the data source, and the method of mapping knowledge domain is used to visually analyze the research hotspots and research trends in this field. The study found that in the field of the combination of ecological environment and GIS, there are a few scholars who have a cooperative relationship. Most scholars conduct independent research and are not closely related to each other. Through keyword co-occurrence analysis, the study found that scholars mainly conduct research on ecological environment security, sustainable development, and ecological sensitivity. The monitoring of mutant words demonstrates the transformation of research hotspots of GIS in the field of ecological environment in the past 20 years. It has experienced sustainable development ranging from geographic information technology as a supplement to ecological environment to the focus on soil erosion, to the focus on ecosystem evaluation process of transformation.

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Research on the inheritance, innovation and high-quality development of Chinese herbal medicine Atractylodes

SUN Yuan-peng, CHENG Zheng, WU Zhe, LIU Yu-si, SUN Yan-ling

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 203-207. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.040

Abstract （372）

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The medicinal value of Atractylodes was introduced.The main cultural heritage, creative and labeling intellectual property rights related to Atractylodes was summarized. Ecological suitability for planting in poor counties, advantageous counties and production base construction of Atractylodes were analyzed. Mainly based on agricultural intellectual property, important agricultural heritage and production base of genuine medicinal materials, the strategy of high quality development of Chinese herbal medicine Atractylodes in the inheritanceand innovation were discussed, in the context of the strategy of traditional Chinese medicine development and the rural revitalization.

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Study on rural environment governance under the background of rural revitalization strategy：A case study of Shiyan city in Hubei province

TAN Yu, LI Tao, ZHE Zheng-hua, YAN Ren-kai, LIN Shan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 208-213. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.041

Abstract （474）

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The relationship between the rural revitalization strategy and the rural environmental governance was expounded.The difficulties and challenges faced by rural environmental governance at present were analyzed.The main measures and successful experiences of rural development and environmental governance in Shiyan city were summarized. On the basis, the corresponding countermeasures and suggestions were put ted forward.

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Research on farmers′ professional cooperatives participating in rural social governance in ethnic areas：Based on questionnaire survey of 58 cooperatives in Changji prefecture

LIU Cheng-chang, HAN Fang, LIANG Jian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (11): 214-217. DOI: 10.14088/j.cnki.issn0439-8114.2020.11.042

Abstract （362）

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Through questionnaire investigation and interviews, the development status and survey data of 58 farmer professional cooperatives in Changji Hui Autonomous Prefecture, Xinjiang Uygur Autonomous Prefecture were analyzed, and the current status and problems of Changji Prefecture farmer cooperatives participating in rural social governance were discussed in depth. According to the survey results, make corresponding suggestions.

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Research progress of hyperspectral technology for detection of agricultural products maturity

ZHANG Yi-zhi, WANG Rui, ZHANG Wei-feng, LU Xiao-hua, GAO Yuan, GU Yu-min, WANG Da-bin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 5-8. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.001

Abstract （378）

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As an emerging three-dimention spectroscopic technique in combination with the traditional imagine technology, hyperspectral technique can obtain the spectral and imagine information of target object simultaneously, possessing great application potential in detecting the maturity of agricultural products due to its nondestructive and rapid characteristics. The research progress of the application of hyperspectral technique in the maturity of fruits, tomato and tobacco were systematically summarized, the existing problems in application were pointed out, the future improvement measures were proposed.

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Research progress on the toxicity of florfenicol

SUN Ji-chao, DONG Zhen, ZHANG Ji-yu, ZHOU Xu-zheng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 9-12. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.002

Abstract （539）

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Florfenicol is a synthetic broad-spectrum antimicrobial derivative of chloramphenicol. It has a wide antibacterial spectrum and has been used to prevent and control respiratory and digestive diseases in pigs. In clinical application, florfenicol may show various toxic reactions at inappropriate or high dose levels, causing varying degrees of damage to animal digestion, reproductive system and different internal organs. The research progress on general toxicity, special toxicity, ecotoxicity and chronotoxicology of florfenicol was reviewed in order to summarize the toxicity characteristics of florfenicol and provide better guidance for clinical use.

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The changes of stem number and plant height of rice treated with different nitrogen fertilizer levels

ZHAO Li-jun, HUANG Ying-hua, ZHANG Shan-xuan, GUO Zhi-yao, HONG Rui-xia, WANG Yi-lin, XIA Li-sha, LI Yan-da, CHEN Qing-chun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 13-16. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.003

Abstract （472）

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In order to clarify the variation of different nitrogen fertilizer levels, stem number and plant height of different rice （Oryza sativa L. ）varieties, a randomized block experiment of different nitrogen fertilizer levels and early construction was set up. The results showed that under the different nitrogen fertilizer levels, the results of the two varieties of Hemeizhan and Yuenongsimiao were consistent for the early cropping and late cropping. The results showed that the number of stems and plant height increased with the increase of nitrogen application rate. In the tillering stage and the jointing stage,there was a extremely significant positive correlation between early cropping and late cropping nitrogen application rate and stem number, and a significant positive correlation or extremely positive correlation between booting stage and yellow maturity stage. Early cropping and late cropping nitrogen application and natural plant height, physiological strain there was a significant positive correlation or extremely positive correlation between the tillering and the booting stages and a extremely significant positive correlation between the jointing stage and the yellow maturity.

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Study on the variation of plant height and stem thickness of sweet corn under different nitrogen fertilizer levels

ZHANG Shan-xuan, HUANG Ying-hua, ZHANG Hua-jie, ZHOU Li-yan, ZHAO Li-jun, XIA Li-sha, HONG Rui-xia, CHEN Qiu-yu, CHEN Qing-chun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 17-21. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.004

Abstract （431）

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In order to clarify the effects of different nitrogen fertilizer levels on plant height and stem diameter of sweet corn, six nitrogen fertilizer levels plot experiments were carried out in Meitian village base of Crop Research Institute of Zhongkai University of Agriculture and Engineering for the early-cropping and late-cropping. The plant height and stem diameter of sweet corn at different growth stages were measured. The results showed that plant height and stem diameter of sweet corn increased with the advance of its growth period, and reached the maximum at the filling stage. There was a significant positive correlation between stem diameter and nitrogen fertilizer; There was a significant positive correlation between early stem diameter and nitrogen fertilizer; Late stem diameter was positively correlated with nitrogen fertilizer; In different growth stages, stem diameter was positively correlated with natural plant height or physiological plant height, and early stem diameter was positively correlated with natural plant height or physiological plant height. Late-growing stem diameter was positively correlated with natural plant height or physiological plant height.

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Analysis of forecast model of returning green stage of winter wheat in Hebei province

SHAN Kun, YANG Shuai, LUO Jing, LI Qian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 22-26. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.005

Abstract （397）

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Using observed data from 11 agrometeorological stations in Hebei province from 1981 to 2018, the forecast models of returning green stage of winter wheat were constructed in Hebei province based on the methods of linear regression and grey system model GM （1, 1）, and the performance of the simulation effect of forecast models was evaluated by historical data. The results showed that the linear trend simulation curve of the returning green stage of winter wheat in each station was insignificance. The grey system model G （1,1） is used to simulate the winter wheat returning to green period in each station, and the simulation accuracy of 10 stations out of 11 stations is above the second level. By analyzing the correlation between the accumulation temperature sequences and the returning green stage of winter wheat, it was determined that the negative accumulation temperature from October 1 to February 15 of next year was the key influencing factor of the returning green stage of winter wheat. The linear regression equations of the negative accumulation temperature of key period and integrated sequence of the returning green stage of winter wheat of all stations reached a very significant level. The probability of an absolute error of less than one week（7 days） of single station linear trend mode, grey system model GM （1,1）,and the linear regression model for all stations with negative accumulation temperature as the dependent variable were 73%, 94%, and 80%. The prediction model of the returning green stage of winter wheat can be constructed based on the grey system model GM （1, 1） under the condition that the change of data is relatively stable. When there is mutation fluctuation in data, the prediction model can be constructed by linear equation at single station. Under the background of climate change,the prediction of winter wheat greenback period by negative accumulated temperature in critical period is suitable for regional analysis based on the linear regression equation of the comprehensive data series of various stations.

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General investigation and statistical analysis of crop germplasm resources in Zigui county

SONG Fang, WU Li-ming, TIAN Rui, HE Li-gang, WANG Zhi-jing, JIANG Ying-chun, JIAO Chun-hai

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 27-32. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.006

Abstract （366）

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Crop germplasm resources is the basis of crop breeding, genetic research and ecological stability, and it is considered as the national strategic resources for its importance in food security and agricultural sustainable development. Based on the statistical analysis of the data from the general investigation of crop germplasm resources in 1956, 1981 and 2014, a comprehensive comparation and analysis about the agricultural production structures, crop planting configurations, crop variety structures and local characteristic resources of Zigui county are performed. The results will not only provide the reference data for the development and planning of Zigui county agricultural industry, but also establish the basis for the utilization of the characteristic resources, the conservation of rare germplasm resources and the improvement of income level of mountain people.

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Analysis of temperature change characteristics in Ezhou city in recent 60 years under the background of climate change

HUANG Si-xian, WANG Bao, CHEN Zheng-hong, ZHAI Hong-nan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 33-39. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.007

Abstract （428）

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Based on the daily temperature data of Ezhou city from 1959 to 2018, the variation characteristics of the average temperature, the average maximum temperature, the average minimum temperature, the extreme maximum temperature and the extreme minimum temperature in recent 60 years in Ezhou city were analyzed by means of linear regression, Mann-Kendall mutation test, cumulative anomaly method and wavelet analysis. The results showed that in recent 60 years, the average temperature, the average maximum temperature, the average minimum temperature, the extreme maximum temperature and the extreme minimum temperature in Ezhou all showed a fluctuating upward trend. The upward trend of the average minimum temperature and the extreme minimum temperature was more obvious than that of the average maximum temperature and the extreme maximum temperature. The annual mean temperature had no obvious mutation; The annual maximum temperature had mutation in 2002; The annual minimum temperature had mutation in 1989; The annual extreme maximum temperature had mutation in 2008; And the annual extreme minimum temperature had mutation in 1981. The annual mean temperature showed quasi-18-year, quasi-10-year interdecadal cycle and quasi-4-year interdecadal cycle; The annual maximum temperature showed quasi-16-year interdecadal cycle and quasi-4-year interdecadal cycle; The annual minimum temperature showed quasi-30-year, quasi-18-year and quasi-10-year interdecadal cycle, quasi-4-year interdecadal cycle; And extreme maximum temperature showed quasi-12-year interdecadal cycle and 4~6 years and 2~4 years interdecadal cycle. The extreme minimum temperature showed quasi-30-year, quasi-16-year and quasi-10-year interdecadal cycle, quasi-6-year and quasi-4-year interdecadal cycle.

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Analysis of the relationship between the meteorological and hydrological drought in Mabie river basin based on SPI and SRI

ZENG Bi-qiu, XIE He-hai, ZHA Da-wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 40-44. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.008

Abstract （388）

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Based on the monthly precipitation and runoff data in Mabie river basin during 1982—2015, the characteristic and variation of hydrological and meteorological drought are analyzedby using standardized rainfall index （SPI） and standardized runoff index （SRI）, and then the correlation between meteorology and hydrological drought are explored. The results show that different degrees of drought occurred every 2~3 years in the Mabie river basin during 1982—2015. The trend of meteorological drought is not significant at the annual scale. At the seasonal scale, the trend of meteorological drought in autumn increases significantly, and the trend of drought in spring, summer and winter is not significant. At the annual scale, hydrological drought has a significant increase trend. After 2000, the frequency of hydrological drought increases significantly, and the number of drought years accounts for 70% of the number of hydrological drought years from 1982 to 2015. There is a close correlation and time-delay relationship to some extent between meteorology and hydrological drought in the Mabie river basin. Among the time scales of 1, 3, 6 and 12 months, the correlation between SPI₁₂ and SRI₁₂ is the best at 12 months, and the correlation coefficient between the SPI₁₂ and the SRI₁₂ of one-month lag is the largest, indicating that the occurrence of a hydrological drought lagged behind the meteorological drought for one month.

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Simulation study on electric field of thunderstorm of Erqi Yangtze river bridge in Wuhan

FAN Min-qi, LI Zheng, CHEN Ren-jun, FENG You-hua

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 45-50. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.009

Abstract （372）

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In order to study the effect of the construction of Erqi Yangtze river bridge on electric field of thunderstorm, the BEM （Boundary Element） method, based on the IES electromagnetic field simulation software, which is used to simulate and analyze the change of the electric field before and after the construction. The influence of the height of different negative charge centers on the main tower and the ground electric field strength is further analyzed. The electric field variation of the main tower is simulated with the lightning leader step by step, which is assumed eight different locations of lightning leader. The spatial distribution of the electric field is given, the characteristics of the electric field variation are analyzed, and then the possibility of lightning strike point is analyzed. The results show that after the bridge is built, the electric field intensity on the top of the bridge tower increases obviously; The height of the negative charge area in the thundercloud decreases gradually, and the electric field intensity on the ground increases gradually; The relative position of the lightning leader and the main tower of the bridge determines the most likely lightning strike point.

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Study on regionalization of soybean drought risk in Henan province

LI Cong, SUN Wan, LIU Jia, LI Tong-xiao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 51-55. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.010

Abstract （306）

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Based on the observed climate data of 118 stations from 1981 to 2014,the soybean data of 4 agrometeorological observation stations and soybean planting area, beans planting area,cultivated area and irrigation area of 118 counties from 2000 to 2010 in Henan province, thesoybean drought risk was zoned in Henan province by using drought grade index and natural disaster risk index. The results showed that the high risk regions of soybean drought in Henan provincemainly distributed in most of Zhoukou, the east of Shangqiu, Xinxiang and the west of Puyang. The highest drought exposure of soybean in Henan province was mainly distributedin Yongcheng of Shangqiu,followed by western Henan, southeastern Henan, southwestern Henan and Changyuan. The regions with strong soybeandrought tolerance in Henan province were mainly located in the north, the east of Henan, and the parts of southHenan, while those weak regions were the mountain areas of westHenan, southwest Henan, and the areas of Xinmi, Fangcheng. The comprehensive risk distribution of soybean drought in Henan province was higher in the west than in the east, and higher in the south than in the north. In general, except the western mountainous areas, the higher drought risk of soybean were in the large soybean planting areaslike most part of Zhoukou, the east of Shangqiu, the east of Nanyang, Changyuan of Xinxiang, parts of Fanxian in Puyang; While the soybeandrought risk relatively low in the most parts of northHenan, south Henan, and Kaifeng, Luohe, Zhumadian, small parts of Shangqiu.The high comprehensive soybean drought risk areas, the soybean production should strengthen scientific and technological research and pay more attention to soybean drought disaster prevention and mitigation.

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Geochemical assessment for land quality in Honghu city

ZHENG Xiong-wei, WANG Shan, HONG Bo, SUN Wei-guo, ZHANG Yuan-pei, LUO Jun-qiang, XU Jing-yin, WU Ying, ZHENG Guo-quan, HU Qing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 56-60. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.011

Abstract （363）

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Based on the data of land quality survey in Honghu city, an evaluation system composed of soil nutrient index and environmental index are established. According to The land quality geochemical assessment specification （DZ/T 0295-2016）, the land quality geochemical assessment and spatial distribution of the land are studied. The soil in Fengkou town, Wanquan town of Honghu city is divided into five gradesHigh quality, good, medium, poor, inferior. The results show that the overall land quality status of the evaluation area is in good condition, with land of high quality and above accounting for 80.91% of the evaluation area, among which land of high quality accounted for 54.01%.

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Comparative study on the soil cadmium enrichment effects of seven indigenous plant species

WANG Zhen-yan, DING Jun, SUN Xiang-hui, ZAHO Jing, CHEN Wan-qiu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 61-63. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.012

Abstract （363）

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Aiming at the present serious situation of soil Cd pollution in Wangcun town and around areas, Xinxiang city of Henan province, 7 native plants with good growth potential were selected and their cadmium enrichment characteristics and remediation effects were compared. The results showed that herbaceous plants had a stronger tolerance to Cd in contaminated soil than shrubs or trees on the whole. However Lagerstroemia indica L. presented a notable enrichment ability and transfer coefficient; The content of Cd in root of Setaria viridis （L.） Beauv. was 82.5 mg/kg, and the enrichment coefficient of root was 3.149; The content of Cd, transfer coefficient and enrichment coefficient of each part of Amaranthus tricolor L. were superior to other plant species. According to the comprehensive analysis, Amaranthus tricolor L., Setaria viridis （L.） Beauv. and Lagerstroemia indica L. all can be used as Cd high enrichment plants for soil remediation in this area.

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Effects of new leaf fertilizer on growth, yield and quality of flue-cured tobacco

DONG Hua-fang, LUO Mao-ping, CHEN Yu-feng, KE Yi-wu, LI Jun-ye, REN Yong-bo, ZHENG Chuan-gang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 64-67. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.013

Abstract （351）

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Using Yunyan 87 as the experimental variety, the fertilizer efficiency comparison test was carried out in Xia village, Yimen town, Huili county, Liangshan prefecture. The results showed that the new leaf fertilizer had no significant effect on botanical characters and main growth period of flue-cured tobacco, but had obvious effect on agronomic characters （stem circumference, leaf area, plant height）. After applying leaf fertilizer, the stem circumference, plant height and leaf area of flue-cured tobacco were larger than those of conventional fertilization control （CK2）; There were significant differences in total sugar between upper, middle and lower leaves of flue-cured tobacco, but there were no significant differences in total nitrogen, nicotine, chlorine and potassium between flue-cured tobacco leaves and CK2, which could improve the quality of flue-cured tobacco leaves but not significantly, and could increase the yield, the output value and the proportion of middle and upper class tobacco leaves.

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Remediation effect of different passivation agents on cadmium in phosphogypsum contaminated soil

WANG Xian-hui, LONG Tao, ZHANG Jian-kun, ZHOU Chun-zhuang, TANG Yun-lai, LIU Ji-kai

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 68-71. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.014

Abstract （377）

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In order to understand the remediation effect of passivation agents on the phosphogypsum contaminated soil, a pot experiment was carried out to investigate the effect of different combinations of passivation agents [iron scraps（I,I1 and I5 represents 1% and 5% respectively）, flyash（F,F1 and F5 represents 1% and 5% respectively） and superphosphate（S,S1 and S5 represents 1% and 5% respectively）]on remediation of cadmium in phosphogypsum contaminated soil, and on the growth as well as the cadmium accumulation of maize seedlings. The results showed that except for I5+F1+S1 and I5+F5 treatments, which could inhibit the plant height and dry weight of up-ground parts of maize seedlings, other treatments had no significant effect on the growth of maize seedlings. All treatments tested could increase the electrical conductivity of phosphogypsum contaminated soil, besides, I1+F5, I5+F1+S1 and I5+F5 treatments could also dramatically increase the soil pH. All treatments could decrease the available cadmium concentrations in soil. I5+S5, I5+F1+S1 and I5+F5 treatments had the biggest drop compared with CK, which were 39.13%、45.65% and 41.30%, respectively. All treatments could decrease the cadmium concentration of up-ground parts of maize seedlings. Compared with CK, the cadmium concentration of up-ground parts of I5+S5, I5+F1+S1 and I5+F5 treated maize seedlings decreased by 29.47%, 21.95% and 31.71%, respectively. All treatments could effectively reduce the activity of cadmium in phosphogypsum contaminated soil. In addition, I5+S5 treatment could significantly decrease the cadmium accumulation of maize seedlings without effecting their growth.

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Study on the combined pretreatment and double membrane process for the treatment of phosphorous tail water in pesticide plant

BAI Zu-guo, PENG Wen-bo, XIE Jing, DING Bang-chao, YANG Ji-heng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 72-74. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.015

Abstract （353）

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The study on the secondary biochemical effluent of agricultural pharmaceutical factory by using pretreatment, ultrafiltration and nano-filtration technology are carried out. By changing the dosage, pressure and recovery rate, the optimum process conditions of the secondary biochemical effluent of agricultural pharmaceutical factory discussed by using pretreatment and double-membrane process technology. The results show that the pretreatment effluent effect meet ultrafiltration water requirements under the condition of PFS dosage 300 mg/L. The membrane system runs stably when the ultrafiltration operating pressure control in 0.1~0.2 MPa, recovery rate 90% and nano-filtration operating pressure control in 2.0~2.2 MPa, recovery rate 80%. After the double-membrane treatment, wastewater TP decreases to 8 mg/L, the removal rate of COD is above 90%, the effluent turbidity is less than 0.1 NTU. Effluent quality reaches the level of new emission standards.

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Study on the impact of long-distance water diversion project construction on ecological environment along the route

ZHANG Ru-yu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 75-78. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.016

Abstract （410）

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Taking the Lianyungang Shugang Channel combined with water supply project as the research object, the influence on the ecological environment of the land area and water area during the construction period and operation period is analyzed. The results show that the project construction covers a total area of 302.13 hm², the construction surface is bare, and the natural system production capacity suffers a certain loss; The implementation of the project reduced the biomass of the regional natural system by 10 855.8 t, accounting for 0.51% of the total biomass of the entire study area; After the completion of the project, the river flow rate and self-purification capacity are increased, and the water quality is improved. The vegetation recovery rate of the whole project area reaches 97%, and the coverage rate of forest and grass reaches 22%. By increasing surface vegetation and improving soil properties, soil permeability can be increased and soil erosion can be reduced.

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Occurrence regularity and evaluation of biocontrol effect on thrips in flue-cured tobacco at seedling stages

XIE Yong-hui, ZHAN You-guo, WANG Zhi-jiang, ZHANG Yong-gui, YU Ping, XU Dong-ya, OUYANG Jin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 79-81. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.017

Abstract （548）

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In order to define occurrence regularity of thrips in flue-cured tobacco at seedling stages, and explore techniques of releasing predatory mites against thrips in flue-cured tobacco at seedling stages, both the population dynamics of thrips and optimum release pattern of predatory mites in seedling stage of flue-cured tobacco were systematically studied. The results showed that after the emergence of flue-cured tobacco, the harm of thrips could be seen, and the population density increased gradually until reaching the peak before transplanting （about amount of 29.34 per 50 strains）, which decreased sharply after transplanting; The quick action of flaky release was significantly better than that of punctate release, but the lasting effect was significantly worse than that of punctate release; After the 40 days of punctate release, no matter in terms of population decline rate or control effect, the punctate release method was better than the flaky release method. When using predatory mites to control thrips in flue-cured tobacco at seedling stage, they should be released as soon as possible after emergence; When the number of thrips population in the seedling stage was large, it was recommended to use the surface release method to control the thrips; When the number of thrips population in the seedling stage was low, it was recommended to use the punctate release method to control the thrips.

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Relationship between dynamics of root-knot nematode and soil environment in Enshi tobacco growing areas

XIANG Bi-kun, SHI He-li, CHEN Hong-hua, ZUO Mei, PENG Wu-xing, YIN Zhong-chun, MENG Gui-xing, TAN Jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 82-84. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.018

Abstract （409）

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The occurrence dynamics of root-knot nematode and its relationship with soil environment were investigated in Enshi tobacco growing areas. The results showed that the tobacco plants began to be damaged by root-knot nematodes 30 d after transplanting, and the incidence and disease index gradually increased until the harvest of tobacco leaves were over. At the same time, the relationship between root-knot nematode and soil water content, soil pH and soil temperature were studied. During the growth and development of tobacco plants, the incidence and disease index of root-knot nematode showed a significant negative correlation with soil water content, and the correlation coefficients were -0.728 and -0.667,respectively; There was a significant positive correlation with soil temperature, the correlation coefficients were 0.593 and 0.536,respectively; There was no significant correlation with soil pH. However, the number of eggs and second instar larvae had no correlation with soil water content, soil pH and soil temperature.

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Study of plant community characteristics, diversity on campus：A case of Guizhou Education University

XIAO Dong-dong, LIAO Xin-rong, SHANG Hai-long

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 85-89. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.019

Abstract （411）

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Taking the plant community in the hills of Guizhou Education University as the research object， the characteristics of plant community， plant diversity and flora were analyzed by the national standard method， and the synarthropic plants in the community were analyzed by means of documentation. The results showed that the study area mainly consisted of Pinus massoniana Lamb. community， coral holly thicket community， Ligustrum lucidum secondary forest community， Platycarya longipes secondary forest community and Diospyros kaki Thunb. community from northeast to southwest. There were 205 species of wild seed plants in 76 families and 154 genera in the research area. According to the geographical composition， the generawas divided into 14 major sub-types and variants. Synarthropic plants accounted for more than 30% of natural vegetation in the region， indicating that Guizhou Education University， wild seed plants were greatly affected by human activities.

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Effects of substrate and shading on growth and photosynthetic physiology of Alnus formosana

TAN Zhang-qiang, HUANG Zhi-ling, SHEN Wen-hui, HAO Hai-kun, CAO Yan-yun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 90-93. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.020

Abstract （368）

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To explore the substrate and environmental conditions more suitable for cultivating Alnus formosana seedlings, seedling substrate was prepared with red core soil, coconut bran, controlled release fertilizer, organic fertilizer and calcium magnesium phosphate fertilizer, and potted seedlings of Alnus formosana were cultivated under different shade conditions. The results showed that the seedling height, ground diameter, Pn, Gs and Tr of Alnus formosana seedlings all showed a light transmittance of 40%>100%>15%, Ci showed a light transmittance of 15%>100%>40% under different shading conditions. The addition of coconut bran in seedling substrate was more beneficial to the improvement of seedling height, diameter and Pn of Alnus formosana. The effects of different fertilizers on Alnus formosana were different under different shading conditions. All fertilization treatments showed that organic fertilizer > slow release fertilizer > calcium magnesium phosphate （fertilizer） under the condition of 40% and 100% light transmittance. Slow release fertilizer could promote the growth of Alnus formosana, but organic fertilizers do not work better under the condition of 15% light transmittance. The optimal treatment for cultivating Alnus formosana seedlings was 40% light transmittance +69.2% red mud +30% coconut bran +0.8% organic fertilizer.

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Effects of different fertilization treatment on the growth and nutrient accumulation and chemical components of flue-cured tobacco

GAO Jia-ming, ZHANG Ji-guang, REN Xiao-hong, DING Cai-fu, LI Fang-ming, SHEN Guo-ming, GAO Lin, LIU Yue-dong, WANG Da-bin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 94-98. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.021

Abstract （418）

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Using the Yunyan 87 as experimental materials, a field experiment was conducted by setting three experiment groups of different NH₄⁺/NO₃^- ratio（3∶7, 5∶5 and 7∶3） to systematically study the effects of different NH₄⁺/NO₃^- ratio on the growth （agronomic trait, biomass）,nutrient accumulation（the content and accumulation of nitrogen, phosphorus and potassium）and chemical components of tobacco in Enshi city. The results showed that there were no significant difference for agronomic trait and biomass among these three groups during the early, middle and late period of tobacco. Likewise, at topping and mature period, there were no obvious difference for the content and accumulation of nitrogen, phosphorus and potassium among these groups. With the increase of the ratio of NH₄⁺/NO₃^-, the content of total sugar and reducing sugar in the upper leaves decreased, while the content of total nitrogen and nicotine increased, while the content of potassium and chlorine did not change significantly. The application of different ratio of NH₄⁺/NO₃^- had no obvious effect on the growth and nutrient accumulation of tobacco plants, but had some effects on the chemical composition of upper tobacco leaves, among which the coordination of chemical composition was the best when the ratio of NH₄⁺/NO₃^- was 3∶7.

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Study on the introduction and cultivation of 20 Chinese rose varieties in Chongqing city

LI Ling-li, WANG Feng, YU Zhi-yong, ZOU Shi-hui, ZHOU Li

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 99-102. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.022

Abstract （673）

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：20 Chinese rose（Rosa chinensis） varieties were introduced for the observation of flowering and growth, and which were divided into 8 early flowering varieties, 6 middle flowering varieties and 6 late flowering varieties according to the full-blossom period. In addition to Mozart, the other 19 varieties can adapt to the climatic conditions of Chongqing city.

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Analysis of soil nutrients in tobacco area of Nanping city and its relation to the quality of flue-cured tobacco

HE De-liang, YANG Zi-jun, ZHANG Shi-xiang, SHEN Hong-tao, XU Chen-sheng, LI Guang-zhao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 103-109. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.023

Abstract （395）

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In order to explore the influence of soil on the characteristic quality of tobacco leaves in Nanping city tobacco growing area, K326 flue-cured tobacco leaves and tobacco planting soil in Nanping main tobacco producing area were used as materials to determine the content of soil organic matter, available nutrients, mineral elements and smoking indexes of tobacco samples, and their correlation was analyzed. The results showed that there were some differences in the content of soil components in different tobacco growing areas. On the whole, soil acidity and alkalinity were suitable, but chlorine content was generally low, soil organic matter content was medium to rich, soil total nitrogen level was low, magnesium content was rich in exchangeable cations, calcium content was low, phosphorus content was rich, potassium content was low. The overall aroma quality of tobacco leaf was good, permeability was prominent, aroma content was sufficient. The flue gas concentration was slightly higher, the fineness was better, the impurities were slightly lower, the irritation was smaller, and the aftertaste was cleaner. The permeability of flue-cured tobacco was negatively correlated with soil pH, positively correlated with soil organic matter, and positively correlated with soil alkali-hydrolyzed nitrogen, exchangeable calcium and available potassium.

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Study on the processing suitability for Congou black tea of Echa No.1

MA Meng-jun, CHENG Fan-yang, GUO Zhi-ming, YU Zi-ming, CHENG Chang-song, TU Xiu-liang, KE Jin-xian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 110-113. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.024

Abstract （529）

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Echa No.1 fresh tea leaves were analyzed for the processing suitability for Congou black tea in terms of sensory evaluation, the main biochemical components andβvolatileβcompounds.βTheβresultsβindicatedβthat the contents of water extracts, teaβpolyphenols, free amino acids, and solubleβsugar were 47.3%, 16.2%, 3.2% and 3.3%, respectively. Theβcontentsβof thea?avins, thearubigins andβtheabrownins were 0.6%, 6.1% and 7.2%. The GC/MS results showed that 51 aroma components were identified. Among the compounds identified, there were 11 kinds of aroma components whose OAVs reached over 1, including β-damascenone, β-ionone, linalool and its oxidation products, geraniol, decanal, nonanal, benzeneacetaldehyde, octanal and limonene. The sensory evaluation results showed that the aroma was sweet and floral, and the taste was mellow,Echa No.1 was suitable for processing congou black tea with high quality.

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Study on Ca²⁺, Mg²⁺ concentration in crab meat before and after molting stage of Scylla paramamosain by ICP-MS

ZHOU Dong-ren, LIN Feng, SUN Bo-yi, SHENG Peng-cheng, GAO Sheng, HAO Gui-jie, YE Xue-ping

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 114-116. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.025

Abstract （327）

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The meat samples of Scylla paramamosain were treated by graphite microwave digestion. ICP-MS was used to determine Ca²⁺ and Mg²⁺ simultaneously. The correlation coefficient of standard curve was ≥ 0.995. The pretreatment time was short and the digestion effect was good. The results showed that the average contents of Ca²⁺ and Mg²⁺ in S. paramamosain were 471 and 2 508 mg/kg in hard shell stage, 385 and 1 565 mg/kg in molting stage respectively. there had no significant differences in Mg²⁺, and had significant difference in Ca²⁺ concentration before and after molting stage. In order to make the Scylla molt and harden smoothly during the growth of the crab, it was suggested that Ca²⁺, Mg²⁺ should be supplemented reasonably in the culture pond.

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Effects of different medicine on hatching rate of fertilized eggs and initial diets on survival rate of larva of Papiliochromis ramirezi

HUANG Yi, HONG Yu-hang, WANG You-pan, PENG Hai

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 117-120. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.026

Abstract （682）

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In the present study, the effects of three different medicine （compound gallnut, gentamicin and furacilin） on hatching rate of fertilized eggs and three initial diets （egg powder, paramecium and artificial ultrafine powder） on survival rate of larva of Papiliochromis ramirezi were investigated. The results showed that the compound gallnut group had the highest hatching rate with （81.22±6.52）%, which was significantly higher than others（P<0.05）. Therefore, the hatching rate of fertilized eggs from Papiliochromis ramirezi could be improved remarkably by using the compound gallnut. In addition, the survival rate of larva in group of artificial ultrafine powder was significantly higher than others with （91.33±3.06）%（P<0.05）. It indicated that artificial ultrafine powder is more suitable for Papiliochromis ramirezi as an initial diet.

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Treatment of compound rhubarb purifying solution on rats with chronic renal failure

WEI Zhen-yuan, YANG Zheng-teng, SHI Wei, HUANG Quan-fang, GAN Dian-hui, CHEN Chao, GONG Mian-yang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 121-123. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.027

Abstract （395）

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In order to explore the therapeutic effect of compound rhubarb purifying solution enema on chronic renal failure rats, it was divided into pathological control group, medicinal charcoal group, large, medium and small dose of compound rhubarb purifying solution enema treatment group by copying chronic renal failure model, the medication period was 58 d. The changes of serum creatinine, urea nitrogen, urine creatinine, red blood cell count, hemoglobin content, and renal histopathology were observed. Results showed that the blood muscle crispness and urea nitrogen of the compound rhubarb purifying solution in each dose group and the medicinal charcoal group were significantly reduced （P<0.05）, and the decline was more significant in the large and medium dose groups （P<0.05）. The 24-hour urinary muscle crunch in each dose group was significantly increased （P<0.05）, and it was significant in the large and medium dose groups （P<0.05）; the red blood cell count and hemoglobin content in the large and medium dose groups of compound rhubarb cleansing solution were significant increased （P<0.05）; each dose group of enema formula can improve renal pathological changes in rats with renal failure to varying degrees. Compound rhubarb purifying solution had certain effects on improving renal function, renal pathology, and anemia in rats with chronic renal failure, and had a certain amount of effect.

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Effects of capsaicin on production performance, egg quality and antioxidant capacity of laying hens

GU Yun-feng, ZHOU Yan-min, TAO Zheng-guo, CHENG Zong-jia, WEN Chao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 124-128. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.028

Abstract （433）

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A total of 720 40-week-old Hyline Brown laying hens were randomly divided into 5 groups. The control group was fed a basal diet, and the experimental groups were fed the basal diet supplemented with 50 mg/kg （Group 1）, 100 mg/kg （Group 2）, 150 mg/kg （Group 3）, and 200 mg/kg （Group 4） capsaicin for 56 days, for investigating the effects of capsaicin on the production performance, egg quality and antioxidant capacity of laying hens. The results showed that compared with the control group, the average daily feed intake of laying hens in Group 1 and Group 2 was increased significantly in the 8th week （P<0.05）. The egg production rate, average egg weight, and feed-to-egg ratio did not change significantly, but the egg production rate had a certain improvement in the 8th week. The color of egg yolk and eggshell thickness in Group 1 were significantly increased in the 8th week （P<0.05）, and the eggshell thickness in Group 2 was significantly increased in the 8th week （P<0.05）. The yolk cholesterol content in Group 1 and Group 2 significantly decreased in the 4th week （P<0.05）, and cholesterol content in Group 2 was significantly decreased in the 8th week （P<0.05）. Yolk fat and cholesterol content were significantly decreased in Group 4 in the 8th week （P<0.05）. The activity of T-SOD in egg yolk in Group 1 was significantly increased in the 4th and 8th week （P<0.05）. The activity of serum T-SOD in Group 2 was significantly increased in the 8th week （P<0.05）. The content of yolk and serum MDA in Group 3 was significantly decreased in the 8th week （P<0.05）, and serum T-SOD activity was significantly increased （P<0.05）. T-SOD activity of egg yolk in Group 4 was significantly increased in the 8th week （P<0.05）. It is concluded that the addition of different levels of capsaicin in the diet slightly improved production performance and egg quality of laying hens. Moreover, it could improve the antioxidant capacity of laying hens.

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The population and distribution status of wild green peafowl in Xinping county,Yunnan province

WANG Fang, TANG Yong-jing, ZHANG Qiao-guan, YAO Chong-xue, ZHANG Zhi-zhong, JIANG Gui-lian, CHEN Ming-yong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 129-133. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.029

Abstract （499）

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From January to December in 2017, a exhaustive investigation has been carried out on the population and distribution of wild green peafowl by visiting survey, line-transect method and trap camera methods in Xinping county, Yunnan province. The results showed that there was indeed a distribution of wild green peafowl in Xinping county, which was mainly distributed in 6 areas of 5 townships with a total population of 126~163. Through investigation and statistics, the total area of existing wild green peacock habitats in Xinping county was 14 511 hm².

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Response surface methodology for optimizing shaking extraction of oil from passion fruit seeds and analysis of fatty acid components

QIN Wen-xia, LI Chang-bao, XIN Ming, WANG Hai-bo, ZHENG Juan-mei, WANG Jing, WANG Jian-gang, HUANG Hui-ling

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 134-138. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.030

Abstract （408）

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The yield of passion fruit （Passiflora coerulea L. ） seed oil was used as the evaluation index, and single factor experiments were carried out on the basis of material-liquid ratio, time and temperature. Response surface methodology was used to optimize the extraction conditions of passion fruit seed oil, and the fatty acid composition of the extracted oil was determined and analyzed by gas chromatography-mass spectrometry （GC-MS）. The results showed that the optimum conditions were as follows, the ratio of material to liquid was 1∶30 （m∶V）, extraction time was 20 minutes, shaking temperature was 23 ℃, and the yield of oil in passion fruit seeds was 22.37%. There were 19 kinds of fatty acids in passion fruit seed oil, the relative amount of unsaturated fatty acids was 84.12%, the relative amount of saturated fatty was 15.88%. The main components were methyl passion （60.51%） and methyl trans-9-octadecanoate （22.05%）. The response surface methodology was used to optimize the extraction conditions to obtaina higher yield of passion seed oil. GC-MS analysis showed that the fatty acid composition of passion seed oil is not destroyed, which was a simple, efficient and non-destructive method.

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Optimization of extraction technology of effective components from upper stem and leaf of Polygonatum sibiricum by orthogonal method

YANG Ze-wa, TAN Hua-yingzi, CHEN Qiao-yun, HU Xue-qi, WU Yun, CHEN Zhi-yu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 139-144. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.031

Abstract （431）

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Using the aboveground stems and leaves of Polygonatum sibiricum as raw materials， the effects of solid-liquid ratio， extraction temperature， extraction time and ethanol volume fraction on the yield of flavonoids， polyphenols and polysaccharides were studied by single factor experiment. The extraction process of flavonoids， polyphenols and polysaccharides was optimized by orthogonal test. The results showed that the optimum conditions for extracting flavonoids were the extraction temperature 70 ℃， solid-liquid ratio 1∶80（m∶m）， extraction time 2.5 h， ethanol volume fraction 90%， average yield of flavonoids was 4.01%. The optimum conditions for extracting polyphenols were the extraction temperature 80 ℃， material-liquid ratio 1∶40， extraction time 1.0 h， ethanol volume fraction 60%， average yield of polyphenols was 0.62%. The optimum conditions for polysaccharide extraction were the extraction temperature 80 ℃， solid-liquid ratio 1∶20， extraction time 2.0 h， and the average yield of polysaccharide was 18.61%. The test results showed that the three optimized processes were stable and feasible.

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SPME-GC/MS analysis of chemical components of volatile components from edible chrysanthemum in flowers

CHEN Zhi-xing, YANG Min, YAO Ru-yu, ZHAO Ya-hong, LIU Min-rong, XUE Jian-ping, HUANG Fei-yan, FANG Yu, WANG Qi-yu, HE Shi-feng, YU Lei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 145-148. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.032

Abstract （397）

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Volatile chemical constituents in flowers of chrysanthemum were analyzed by means of top solid phase microextraction gas chromatography/mass spectrometry （SPME-GC/MS）. The results showed that 51 volatile aroma components, including alkenes, esters, alcohols, and a small amount of acids, heterocycles and alkanes. The main component was monoterpenoids α-pinene, β-myrcene, α-terpinene, β-pinene, γ-terpinene, and their relative contents were 74.04%, 5.24%, 3.96%, 3.44% and 2.01% respectively, accounting for 88.69% of the total relative content.

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Study on heavy metal enrichment and risk assessment of Silurus meridoualis and Pelteobagrus fulvidraco in Shatuo hydropower station of the Wujiang river

BA Jia-wen, WEI Yan-wei, YAN Han-lin, XU Cheng-xiang, LI Zhong-li, MEI Jie

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 149-153. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.033

Abstract （453）

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In order to understand the present situation of heavy metal enrichment and food safety of fish in the Shatuo reservoir of the Wujiang river, the heavy metal content in the muscle of Silurus meridoualis and Pelteobagrus fulvidraco were determined by inductively coupled plasma emission spectrometry （ICP-AES） and atomic fluorescence spectrophotometry （HCHG-AFS）. The results showed that the contents of different heavy metals in the muscles of the fish were different, and the content of Zn in two kinds of fish muscles was the highest, and the content of Cu, Cr, Pb and Hg in the muscle of Pelteobagrus fulbate were all higher than that of S. meridoualis. The content of heavy metals in muscle of two species of fish was lower than the national standard. The results of hazard index showed that the human health risk of edible S. meridoualis and P. fulvidraco in the Shatuo reservoir of the Wujiang river was relatively small. The results of single factor pollution index of heavy metals showed that the pollution degree of Pb, Hg and Cd were high in fish from the Shatuo rreservoir, and there was a potential heavy metal pollution risk in the Shatuo reservoir of the Wujiang river.

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Nondestructive detection experiment of typical economic fruit near-infrared diffuse reflection and its spectral data analysis

TAN Bao-hua, XIAO Teng-fei, LIU Qiong-lei, LI Gen, HUANG Cheng-xu, LI Gang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 154-158. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.034

Abstract （406）

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Based on the near-infrared spectrum analysis technology, this paper used the near-infrared diffuse reflection detection method to design an experimental system for the near-infrared detection of fruit sugar content. Taking typical economic fruit fresh jujube and banana as samples, the spectrogram of the whole samples and the spectrogram of the slice samples of the two fruits were studied, and the differences of the spectrogram were analyzed. The results showed that the near-infrared method could effectively realize the nondestructive testing of the fruit samples, and reflect the internal sugar content information of the fruit samples.

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Application of brushless DC motor control technology in freshwater breeding mower

WANG Zuo, ZHANG Jun-feng, GAO Xing-xing, XIAO Jin, LUO You-yi, TIAN Man-zhou, PENG Gui-yuan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 159-163. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.035

Abstract （389）

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In the agricultural freshwater breeding, the appropriate amount of water grass is conducive to fish respiration and can purify water quality, but the excessive amount of water grass will affect the survival and growth of fish. Mechanical mowing is the most widely used mowing method at present. The propulsion system of mowing boat was an important link of mechanical mowing. Brushless DC motor was a typical mechatronic equipment. Using the embedded technology, power electronics technology, analog electronics technology and digital electronics technology, the control system of the mowing boat based on brushless dc motor was designd, and realized the direction control, speed control and mowing control of navigation through hardware design and software design were realized. Through the prototype test, the control system has higher practicability and stability. Compared with the traditional brush DC motors, the mechanical mower with brushless DC motor has a longer life.

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Design of irrigation control system for flower greenhouse based on Modbus-RTU

SHENG Qiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 164-166. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.036

Abstract （425）

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Aiming at the demand of flowers for on-demand irrigation and growth environment and the limited number of analog input and output modules of PLCs, design an irrigation control system based on Siemens S7-200 SMART series PLC. The control system uses Modbus-RTU communication, collects environmental parameters such as inverter, soil and air temperatures and humidities, illumination and soil conductivity, and control the frequency of inverter to realize on-demand irrigation of flowers, and uploads related parameters to MCGS touch screen by industrial Ethernet for fine management.

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Design and application of groundwater dynamic online monitoring system in middle and lower reaches of Tarim river

YANG Lei, HUO Ai-di, GUAN Wen-ke, YI Xiu, DU Wei-hong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 167-170. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.037

Abstract （412）

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Aiming at the current situation of groundwater dynamic online monitoring in the middle and lower reaches of tarim river, the network was effectively used to simplify data processing, increase the function of forecast and warning and optimize the power supply system. The results showed that the equipment installed in the middle and lower reaches of the tarim river has completed the remote transmission of data and data analysis. Based on the 18 d real-time monitoring data, the groundwater level fluctuated between 3.462 and 3.617 m. Based on the table of groundwater level threshold of populus euphratica growth, the groundwater level at this location was between 0.50 and 4.71 m, and has not crossed the threshold of good growth and stress. Therefore, populus euphratica was growing well, and the equipment does not produce early warning information. Thus, the groundwater monitoring system could be used for dynamic monitoring of groundwater level in the middle and lower reaches of Tarim river.

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Bioinformatics analysis of BpPR1 gene from Betula platyphylla

AN Yu-ting, LI Ran-hong, CHEN Xin, LIU Dan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 171-174. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.038

Abstract （374）

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Using bioinformatics methods, physical and chemical properties of white birch BpPR1, hydrophilic/hydrophobic, across the membrane structure, secondary structure and tertiary structure and with some other plants PR1 protein homology were forecasted, the cis function of the gene promoter elements and gene structure prediction and analysis were performed. The results showed that①the total length of cDNA of BpPR1 was 816 bp, and the CDS was 531 bp. The encoding protein was a hydrophilic stable protein composed of 176 amino acids. It had a transmembrane transport structure and belonged to the CAP protein family. Birch BpPR1 was closely related to grape and jujube. ②its genome structure contained 2 exons and 3 introns. ③BpPR1 promoter contained multiple eukaryotic promoter CAAT box and the basic components-TATA box, also contained low temperature response, the response of abscisic acid and methyl jasmonic acid, auxin response and the response of salicylic acid and cytokinin response related components, as well as MYBHv1 binding sites and so on, indicating that the protein may be a variety of hormones control, to participate in the process of biological and abiotic stress.

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Exploring the conditions for efficient electric transformation of Xanthmonas campestris pv. malvacearum （Xcm） V8

WANG Qing, MENG Fan-qi, ZHANG Yong-yue, MIAO Wei-guo, LIU Zhi-jian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 175-178. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.039

Abstract （418）

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In order to obtain efficient electrical conversion conditions, experimentswere conducted to study the efficiency between optimal concentration for 5-AZA and factors playing roles in the efficiency of cotton keratinae [Xanthmonas campestris pv. malvacearum （Xcm）]V8 one by one. The results showed that V8 for preparing competent cells was domestricated by 5-AZA with a concentration of 100 μmol/L at the logarithmic metaphase, Whiah was used to acclimate V8 in the medium logarithm to prepare competent cells. When the guaranteed voltage was 2.1 kV, 7 μL plasmid as well as concentration of 50 μg/mL, recovery media SOC of 4 ℃ pre-cooling, culturing with time of four to five hours by oscillating, the efficiency could reach about 5 ×10² /μg DNA.

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Method on high-throughput DNA extracting of rape hybrid

ZU Feng, LI Xia, HE Xiao-ying, ZHAO Kai-qin, CHEN Wei, SHU Zheng-qi, DONG Yun-song

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 179-181. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.040

Abstract （434）

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Through the deep hole plate germination of Brassica napus hybrid, the steps of DNA extraction by alkali pyrolysis were simplified, and the application of high throughput grinder Fast & Fluid Management SK300 sample homogenizer was combined to provide a whole set of intensive scheme for rapidly obtaining cotyledon DNA samples of rapeseed hybrid with high throughput and low cost. Using this method, 10 thousand DNA could be obtained in 10 hours, which improve the efficiency of DNA extracting, significantly and facilitate purity identification of hybrid rape seed with molecular marker, effectively.

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Study on the grass-roots civil servants’ burnout based on job demands-resources model： The mediation and moderation effects of self-efficacy

LIU Rui, ZHU Feng, FAN Pei-yao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 182-189. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.041

Abstract （474）

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The physical and mental health of grass-roots civil servants have always been the focus of human resources management research in the public sector, but job burnout has been widespread in grass-roots civil servants. In order to alleviate the group’s job burnout and promote the sustainable and healthy development of grass-roots civil servants.This study is based on the job demands-resources model, a theoretical model of job burnout for grass roots civil servants is established. Based on the regression analysis of 504 samples of grass-roots civil servants, the results show that, in terms of job requirements, role stress in job demands positively affects job burnout of grass-roots civil servants; In terms of work resources, job social support and career growth opportunities negatively affect job burnout of grass-roots civil servants; Self-efficacy plays a moderating role between role stress and job burnout of grass-roots civil servants, and strengthen the relationship between them; Self-efficacy plays a part of mediating role among job social support, career growth opportunities and job burnout of grass-roots civil servants.

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Farmers’ cognition and participation analysis of circular agriculture：Based on farmers questionnaire survey in seven cities of six provinces

SONG Hui-min, FU Chen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 190-196. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.042

Abstract （357）

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Agriculture is not only an important industry of national economy, but also an important field of implementing the concept of green development. Developing circular agriculture is an important part of the green development in agriculture. At present, China's circular agriculture has entered a new stage of accelerated development,hundreds of millions of household contract operation farmers are the largest and most important agricultural operators. Their scientific cognition and active participation are the key to accelerate the development of circular agriculture. Questionnaire survey of farmers in seven cities of six provinces was investigated to analyze and master the cognition and participation of farmers in circular agriculture. The statistical data revealed that the cognition and participation level of Chinese farmers in circular agriculture is not high currently. Thus, the study suggested that strengthening publicity to raise awareness, expanding trials to strengthen demonstration, cultivating operators to lead and drive, organizing innovation to link industries.

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Analysis of the demand and expression mechanism of compulsory education of rural residents in China

CHEN Jing-yi, ZHANG Xu-jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 197-200. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.043

Abstract （377）

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From the theoretical level, the demand of compulsory education of rural residents in China includes the exogenous variable of rural compulsory education, the supply and demand mechanism of rural compulsory education, the provision and results of rural compulsory education service, and the evaluation standard of rural compulsory education, which is not only affected by the exogenous variable ,but also affects the evaluation standard. From the practical level, at present, China's “supplier centered” education policy is difficult to meet the compulsory education demand of rural residents, and there exist no effective information communication mechanism between education providers and consumers. Rural residents can only express their education preferences by choosing schools,choosing places or even dropping out of school depending on their own economic conditions, social capital and cultural capital. Therefore, the government needs to reflect on the current supply mode and development logic of rural compulsory education, and establish the education demand expression and information communication mechanism between the government and rural students with their parents, so as to ensure the satisfaction of the education supply results.

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Innovative supporting intelligence mode to block the intergenerational transmission of poverty

WANG Yan, YANG Xiao-ping

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 201-205. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.044

Abstract （371）

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At present, blocking poverty intergenerational transmission is a difficult and complex project, must construct the long-term mechanism by using the means of education, through the transformation of poor family members' ideas, general education and vocational skills training, enhance the participation in practice the skills and abilities, to improve their own “hematopoietic” function, change the poor families hard living conditions, can we truly achieve poverty to get rich. Based on the current causes of intergenerational transmission of poverty, this study discussed the relationship between education and intergenerational transmission of poverty, analyzed the mode of supporting intelligence under the pattern of targeted poverty alleviation, and summarized the important ways to block intergenerational transmission of poverty.

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Efficiency evaluation of China's provincial logistics industry under low carbon economy：Based on three-stage DEA and K-means cluster analysis methods

SU Gui-ying, QIAO Zhu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 206-212. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.045

Abstract （389）

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According to the 2009—2016 national logistics data of 30 provinces （city, autonomous regions）, based on the development concept of low carbon economy, the energy consumption and carbon emissions is input variables, the three-stage DEA model is used to evaluate the efficiency of the logistics industry. The results showed that the overall development level of China was not high, the logistics industry was still in the stage of increasing scale efficiency, there were differences in the development level of different regions. To effectively improve the efficiency of the logistics industry, pure technical efficiency and scale efficiency for the standard, the k-means cluster analysis was carried out for 30 provinces （city, autonomous regions） in China, and put forward corresponding suggestions and countermeasures according to the development level of different types of logistics industry.

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Analysis of the rural revitalization path under the vision of urban and rural equivalence

HUANG Deng-hui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (12): 213-217. DOI: 10.14088/j.cnki.issn0439-8114.2020.12.046

Abstract （322）

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The rural revitalization strategy is the top priority of the party and state work in the new era. The rural revitalization has rich scientific connotation, target requirements and realization path. It is difficult to fully realize urbanization and citizenization, and the realistic background of the unbalanced cultural level between urban and rural areas and regions, so that rural development must pay attention to the development of the village itself and realize the localization of the village. By giving full play to its own advantages and based on the local realities, promoting rural development according to local conditions, industry and people’s conditions, and realizing the rural residents ability to enjoy “different but equivalent” social living standards and welfare benefits for urban residents in the countryside, that is, to achieve urban and rural equivalent.

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Research progress on the application of microbial technology in soil remediation

WU Nan-nan, ZHANG Ke, SUN Chen-xi, GU Xiao-yan, SONG Lei, WANG Xin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 5-9. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.001

Abstract （494）

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The types of soil pollution （organic pollution， heavy metal pollution，radioactive pollution） and microbial remediation technologies were introduced；The application research of the microbial remediation technologies （bacteria method， biological ventilation method， biological culture method， stacking method， prefabricationbed method， bioreactor method） in soil were summarized； The problems existing in microbial soil remediation technology were analyzed and the prospect was put forward.

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Progress in immunological effects and application of CpG ODN nano adjuvant

WANG Zheng-fan, WAN Zeng-pei, GAO Xiao-peng, YING Bi-yun, XIANG Rui, LI Yi-yun, PANG Xuan-fei, LI Zhong-sheng, BAI Ai-quan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 10-13. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.002

Abstract （492）

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CpG ODN is a synthetic DNA sequence with non-specific immunostimulation which simulates the response process of bacterial DNA in vivo, inducing cellular and humoral immunity. CpG ODN has shown great potential as a novel immunopotentiator in veterinary clinical applications, and has attracted much attention in the fields of immunology, pharmacology and pharmacodynamics. However, CpG ODN is unstable and easily digested by nucleases, which are negatively charged and do not easily bind to the cell surface. Therefore, how to efficiently transfer CpG ODN and effectively ingest it becomes a new challenge. The study of nano-transmission system has achieved significant efficacy for CpG ODN alone or in combination with vaccine immunization, and broadened the thinking for improving the application of CpG ODN in vaccine research. The mechanism of action, immunological activity, safety and clinical application of CpG ODN nanoadjuvant were summarized, and future research directions in this field was prospected, to provide useful reference for follow-up work.

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The effect of microRNAs on fat deposition in livestock and poultry and its application in genetics and breeding

LI Xin-yu, AN Qing-ming, WANG Da-hui, HE Hua, FENG Zi-ting, WEN Yi-fan, LEI Chu-zhao, CHEN Hong, HUANG Yong-zhen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 14-18. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.003

Abstract （354）

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The association between genes and characters of livestock and poultry was widely studied. DNA and RNA with coding effect have important effects on traits. In addition, miRNA in non-coding RNA can have a specific effect on livestock and poultry traits by affecting the translation of mRNA. The research of miRNA in fat metabolism of livestock and poultry was summarized, in order to provide reference for further research on the description and functions of miRNA in fat tissue of livestock and poultry.

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Comparative test of new purple sweet potato varieties

YAN Xiao-hui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 19-21. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.004

Abstract （393）

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The yield and characters of seven new purple sweet potato varieties with high quality, including Jinshu 17, Jinshu 20, Funingzi No.4, Funingzi No.5, Longzi No.4, Longzi No.6 and Longzi No.9, while taking Funingzi No.3 as control variety,were compared, in order to provide scientific basis for its production and application in Fujian province. The results showed that the yield of Longzi No.9 was 46.52 t/hm², which was 22.4% higher than control, and ranked the first, and the yield of Jinshu 20 was 42.35 t/hm², which was 11.4% higher than control, and ranked the second among all varieties tested. The yield of Jinshu 17 was 39.17 t/hm², which was only 3.1% higher than control. And these three varieties had good appearance with purple skin, purple meat and high-yield character. The yield of Longzi No.9 and Jinshu 20 was siginifacnt higher than contol variety, so these two vareites was suitable for local planting. The yield of other varieties was all lower than control, the yield of Longzi No.6 and Longzi No.4 was significant or extremely significant lower than control.

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Effects of sowing dates and planting density on yield components of new rice variety Liangyou 3313

FAN Bing, TIAN Yong-hong, FANG Zhen-bing, ZHAO Sha-sha, PAN Gao-feng, WANG Long, CHEN Bo

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 22-23. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.005

Abstract （385）

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The effects of sowing date and planting density on yield components of rice （Oryza sativa L.） variety Liangyou 3313 were studied. The results showed that the suitable sowing date of Liangyou 3313 in northwest Hubei province was the middle of April, and the planting density of 281 250 holes/hm² was the most suitable under the condition of medium and high fertility level.

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Study on the grain shape and quality traits of Fagopyrum tataricum

ZHANG Yi-zhong, DENG Lin-qiong, FENG Tu, ZHANG Shi-long, WU Qiao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 24-27. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.006

Abstract （418）

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The grain shape and quality traits of 26 Fagopyrum tataricum（L.） Gaertn seeds came from Bijie county of Guizhou provine were studied. The results showed that the variation ranges of grain length, grain width, grain thickness, bulk density, 1 000-grain weight, protein content, flavone content, soluble sugar content and water content were 4.10~5.71 mm, 2.81~3.54 mm, 2.71~3.26 mm, 565.54~ -686.84 g/L, 14.54~20.82 g, 6.12%~10.14%, 3.95%~10.60%, 4.15%~16.79% and 11.40%~13.67%, respectively. The average values were 4.62 mm, 3.22 mm, 2.97 mm, 627.46 g/L, 17.28 g, 7.84%, 6.20%, 9.95% and 12.71%, respectively. The correlation analysis showed that there was an extremely significant positive correlation between 1 000-grain weight and grain length, grain width as well as grain thickness. Whereas grain length was significant negative correlation with grain width, 1 000-grain weight and bulk density were positive correlation with grain width and grain thickness, grain length was negative correlation with grain thickness and bulk density. There was a positive correlation between protein content and flavone content as well as soluble sugar content, while protein content, flavone content and soluble sugar content was negative correlation with water content, and between soluble sugar content and water content reached an extremely significant level.

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The preparation and application of soil remediation improver for heavy metal cadmium in farmland

YANG Tao, LI Jian-guo, WEI Lin-gen, PENG Zhi-ping, CHEN Yuan-hua

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 28-30. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.007

Abstract （377）

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A soil remediation improver was prepared for heavy metal cadmium from contaminated farmland in Jiangxi province. The soil remediation improver is a kind of mixed fertilizer formed by mixing biochar and fertilizer in a certain proportion. Taking early and late rice as materals, seven treatments, including five different soil remediation improver treatments and two control of no fertilizer and conventional fertilization,were set up to test whether the soil remediation improver could reduce the cadmium content of rice seed in cadmium polluted farmland. The results showed that the Cd content in rice decreased with the increase of the amount of soil remediation improver,and the yield was no significant difference between soil remediation improver treatments and the conventional fertilization control.

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Preliminary study on the effects of four soil amendments on improving quality and efficiency of flue-cured tobacco

PENG Shi-cheng, LUO Rong-zhao, YU Qian-yuan, CHEN Yu-feng, KE Yi-wu, LI Jun-ye, DONG Hua-fang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 31-34. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.008

Abstract （386）

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The effects of improving quality and efficiency of flue-cured tobacco were studied by adding different kinds of soil amendments （sepiolite, bentonite, diatomite, white marble） and lue-cured tobacco planted without soil modifiers. The results showed that the four soil modifiers had no significant effect on the growth period of tobacco leaves in field. In terms of agronomic characters, the growth of flue-cured tobacco treated with sepiolite was generally better than that of other treatments. The main diseases were climate spot weather fleck disease,brown spot disease and tobacco mosaic disease,and the incidence rate was close. The output value and yield of each treatment were different. The output value of diatomite treatment was higher than that of the control group by 9 832.65 yuan per hectare. The highest proportion of superior tobacco leaves was the treatment with sepiolite. The contents of chemical components treated with white marble and sepiolite were closer to that of high-quality tobacco leaves, and the tobacco quality was the best. Therefore, the application of sepiolite of 2 kg/m² in the tobacco planting soil can effectively improve the botanical characters of tobacco plants and the chemical quality of tobacco leaves, and the output value is also higher than that of the control group.

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Effects of potassium fertilizer application on dry matter, nutrient accumulation and yield of soybean

QI Xin, ZHAO Ya-nan, SI Yu-kun, ZHANG Chuang-ye, HUANG Yu-fang, BAI Hong-bo, YE You-liang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 35-38. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.009

Abstract （372）

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In order to discuss the suitable amount of potassium fertilizer for soybean[Glycine max （L.） Merr.], two-year field trials were conducted in 2017 and 2018 in Linying county, Henan province. Four potassium fertilizer gradients （K₂O application amount of 0, 60, 120, 180 kg/hm²） were set up to analyze the effect of K₂O amount on soybean dry matter, nutrient accumulation, yield, fertilizer utilization rate and economic benefits. The results showed that the dry matter and nutrient accumulation of soybeans increased continuously with the advance of the growth period, and the dry matter accumulation rate was fast from the flowering and pod-forming stage to the grain-filling stage. In 2017, the dry matter weight of mature soybeans increased first and then decreased with the increase of potassium application amount, and the potassium accumulation continued to increase with the increase of potassium application. The dry matter and potassium accumulation of mature period in 2018 both increased with the amount of potassium increases. In 2017, the yield of soybean had no significant difference among all treatments. In 2018, compared with control that without potassium application, the yield of different potassium application treatments increased by 34.3% to 52.7%, and this difference all reached significant level. The yield and increased economic benefit of applying K₂O 120 kg/hm² was the highest. So, the recommended amount of potash fertilizer is about 120 kg/hm² K₂O in the middle Henan province.

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Effects of summer drought and soil humidity on spring corn and winter wheat yield

ZHANG Hong-ni, ZHANG Tian-feng, CHE Xiang-jun, ZHANG Miao-miao, GOU Rui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 39-42. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.010

Abstract （399）

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Using the precipitation data from the eight weather stations of Qingyang LOESS plateau from 1971 to 2018, the spatial distribution characteristics of the precipitation and drought were first analyzed, and the time change laws of precipitation were analyzed. The relationship between drought, spring corn and winter wheat yield was analyzed. It is then used to represent the data of soil relative humidity in the winter wheat field from spring 1994 to 2018 and winter wheat field from 1986 to 2015 at the Xifeng Agricultural meteorological experimental station on the Dongzhi plateau in the loess plateau. The relationship between soil relative humidity and spring corn and winter wheat production is analyzed. The results showed that the lowest precipitation was 123.7 mm in Zhenyuan weather station, 125.3 mm in Huanxian weather station, 162.5 mm in Zhengning weather station, and 149.9 mm in Huachi weather station. Precipitation decreases from East to West. The highest frequency of drought is 41.7% in Heshui, Qingcheng and Huachi, and 31.1% in Ningxian county, and the frequency decreases from northeast to southwest. Precipitation was less in the 1970s, more in the 1980s and 1990s, less in the 2020s, and more in 2011—2018. The average yield of corn per hectare was 960.0 kg/hm² higher than that of the spring of the drought; The average yield height of winter wheat is 1 599.0 kg/hm². The average yield of maize is 2287.5 kg/hm² higher than that of soil relative humidity. The average yield height of winter wheat is 705.5 kg/hm².

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The change of area of cultivated land and main crops in recent 40 years in Ruoqiang county

Gulizere·REHEMAN, Tursun·KASIM

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 43-46. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.011

Abstract （447）

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The changes of cultivated land area, sown area of main crops and red jujube in Ruoqiang county of Xinjiang from 1978 to 2017 were analyzed using land use dynamic degree model. The results showed that, in resent 40a, the area change of cultivated land in Ruoqiang county was the overall expansion of the trend. Since 2001, red jujube area of Ruoqiang county increased rapidly, because of Ruoqiang county took the income of farmers and herdsmen greatly increase as a breakthrough, intensified strategic adjustment of agricultural industry structure, with the construction of the best red jujube base in China as the goal. Because the new cultivated land in Ruoqiang county has adopted the interplanting mode,by planting cotton and grain crops and then cultivating red jujube,so the sown area of cotton, wheat, maize and other crops showed a trend of substantial increase and then decrease.

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Study on the diversity of small and medium-sized soil animals in spring in the Yellow river wetland of Shaanxi province under different land use patterns

LI Wei-na, HU Qing-ling, HAO Zhuan, MENG Qing-lei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 47-50. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.012

Abstract （363）

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In April 2019, three soils samples of different land use patterns were selected and sampled in the Yellow river wetland of Shaanxi province to study the diversity of small and medium-sized soil animals with different land use patterns. The results showed that a total of 51 small and medium-sized soil animals were obtained, belonging to 1 phylum, 2 classes and 9 orders respectively. All the soil animals belonged to Arthropod, among which, Insecta was the dominant group, accounting for 60.8% of the total captured animals, Arachnids accounted for 39.2% of the total captured animals. The diversity index and uniformity index of the small and medium-sized soil animal under three different ways of land use showed obvious difference. The diversity index and uniformity index of riverine wetland in Wei river basin were higher than those of the other two wetland types, and the diversity index and uniformity index of saline-alkali wetland in Luyang lake were the lowest. The reason may be related to the salinization of saline-alkali wetland in Luyang lake and other physicochemical properties of soil quality related.

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Purification effect of eutrophic water by Hyriopsis cumingii’s stocking mode

CHU Tian-jiang, GUO Wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 51-53. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.013

Abstract （347）

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Hyriopsis cumingii’s purifying effect was compared between upper and bottom stocking. The results indicated that Hyriopsis cumingii’s upper and bottom stocking can significantly reduce （P<0.05） the total nitrogen （TN）, the total suspended substance （TSS）, chemical oxygen demand （COD） and chlorophyll a （chla）, but the removal efficiency of total phosphorus （TP） was not significant. The removal efficiency of eutrophic water was not significant between upper and bottom stocking.

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Spatial and temporal relationship between China’s nighttime light index and PM2.5

WANG Gui-lin, GAO Fang, LU Gai, WANG Shi-yan, LI Ri-sheng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 54-60. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.014

Abstract （355）

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Nighttime light index was taken as a measurable spatial index to represent the level of urbanization, and its impact on PM2.5 pollution was explored, meanwhile the spatial and temporal relationship between them was analyzed. The results showed that China’s nighttime light index increased from 7.49% in 2000 to 11.05% in 2010 and 16.21% in 2015, showing a rapid growth trend. Accordingly, the concentration of PM2.5 increased from 32.10 μg/m³ in 2000 to 37.54 μg/m³ in 2010 and 37.44 μg/m³ in 2015. The growth trend of the two was highly consistent. Based on 405 typical urban areas, the nighttime light index increased from 22.48% in 2000 to 48.11% in 2010 and 62.78% in 2015, with growth rate of 179.27%, 1.54 times of the national average. The PM2.5 concentration in 405 typical cities increased from 28.99 μg/m³ to 53.51 μg/m³ in 2010 and 58.53 μg/m³ in 2015, with growth rate of 101.90%, 6.12 times of the national average. The increase of nighttime light index and PM2.5 pollution in typical urban areas was significantly higher than that in non-urban areas, with a significant positive correlation （R²=0.582 8）. Research shows that China’s urbanization process, especially economic development, has a huge impact on urban air pollution, directly exacerbating PM2.5 pollution.

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Characteristics of a fog weather process at Hubei International Logistics Core Hub Airport in December, 2016

WANG Bao, HUANG Si-xian, ZHANG Huo-ping, YANG Hui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 61-66. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.015

Abstract （363）

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A foggy weather process at Hubei International Logistics Core Hub Airport on December 1st to 2nd, 2016, was analyzed by using the data of regular observation, regional automatic observation station and NCEP 1°×1° reanalyzed data. The results showed that the process of fog appeared under the cooperation of the high, middle, low level weather system, and has obvious characteristics of radiation fog. In the fog stage, the 10 m wind on the ground was dominated by a partial east wind with a wind speed of less than 1.0 m/s. The situation that wind speed increased or the wind direction turned to a northerly wind facilitated the dissipation of fog. The vorticity and divergence of the lower troposphere was small, and the middle and lower layers were dominated by downdraft. The relative humidity of groud surface was maintained at about 5 g/kg, and the temperature dew point difference was about 3 °C. The sharp increase in temperature dew point difference can indicate the dissipation of fog. Before the appearance of heavy fog, the low-level relative humidity was maintained more than 60%, a relatively dry areas with relative humidity of less than 20% was maintained between 800 hPa and 600 hPa; There was an inversion layer or neutral layer junction near 950 hPa to 900 hPa, and there was a cold advection at the lower level and a warm advection at the middle and upper levels, the cold advection increased from the ground to the height, then the upper layer turned into the tendency that the cold advection decreased with height and the warmer advection increased with height. The cold flow near 900 hPa decreased with height, forming a stable structure with warm and cold in a smaller range.

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The statistic analysis of rice “3414” fertilizer experiment using R language

FAN Xiao-hui, LIU Wen-ting, CHEN Mu-song, WU Shou-hua, WENG Lin-lin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 67-71. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.016

Abstract （431）

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Using the field experiment scheme of “3414” complete fertilizer effect and the rice variety Ningyouhei 807, the field experiment of N, P, K fertilizer effect was carried out in Xibing town of Fu’an city in order to explore the rice fertilization index system of Fu’an city and guide the local scientific fertilization. The experiment data of fertilizer effect of “3414” were analyzed by R language, and NPK ternary quadratic equation, NK binary quadratic equation and P unitary quadratic equation were successfully formulated. By comparing the ratio of production and investment, the NK binary quadratic equation was the best fitting effect equation. The corresponding fertilization amount were 171.957 kg/hm² for N application, 75.000 kg/hm² for P₂O₅ application, 30.151 kg/hm² for K₂O application, and the theoretical optimum yield was 7 062.188 kg/hm².

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A assessment model of water resources carrying capacity based on random forest algorithm and its application in Hubei province

LU Jia-hui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 72-76. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.017

Abstract （410）

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An assessment system of water resources carrying capacity indicator was constructed according to pressure-state -response （PSR） framework, and the random samples were constructed based on index threshold. On this basis, the random forest algorithm was applied to evaluate the water resources carrying capacity in Hubei province. The results showed that the relationship between the index factors and the water resource carrying capacity could be well fitted by the random forest with a high accuracy. The scheme was of good maneuverability and certain application value. The random forest algorithm can recognize the importance of variable unbiased, and the research showed that the modulus of water demand and the modulus of water supply were the key factors affecting the carrying capacity of water resources in Hubei province. The spatial distribution of water resources carrying capacity in Hubei province was uneven. The carrying capacity of water resources in western Hubei and Xianning areas was of good grade, while that of eastern Hubei and middle Hubei was generalgrade. The Jianghan plain area with Wuhan as the core belonged to poorgrade. It was suggested that regional management of water resources should be strengthened to promote the development of water resources conservation ability and economic society synchronously.

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Study on forecasting and early warning indicators of urban waterlogging in Qingyang

ZHANG Tian-feng, JIANG Hui-feng, ZHANG Hong-fen, WU Ai-min, ZHANG Hong-ni, JIAO Mei-ling, ZHANG Xue-jiao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 77-83. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.018

Abstract （433）

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Based on the precipitation observation data from 1937 to 2018 of the Xifeng National Basic Weather Station in Qingyang city and the waterlogging data recorded in the Qingyang city, the annual precipitation concentration and concentration period, as well as the formula of the intensity of torrential rain were calculated by counting the strong precipitation frequency and the 95%, 99% fractional values total precipitation. Combined with urban drainage network simulated using SWMM system, we aim to analyze and evaluate the risk of waterlogging in the city of Qingyang, and determine the threshold of urban precipitation in Qingyang city as a standard for the release of urban monitoring and warning information. The results show that extreme precipitation is the main cause of urban waterlogging in Qingyang city,which generally concentrates in summer, mostly in July to August. Two factors have positive relationships with extreme precipitation： Total precipitation and intensity. The greater the total precipitation and the heavier the intensity,the more serious of the urban waterlogging disaster. When the total precipitation reaches more than 30 mm,precipitation intensity becomes particularly important：when the precipitation reaches 15 mm in 30 minutes, waterlogging points are prone to water accumulation;When the precipitation reaches 20 mm in 30 minutes, the waterlogging point may have disasters;When the precipitation reaches 30 mm in 30 minutes, the waterlogging point is prone to serious disasters;When the precipitation reaches 40 mm in 30 minutes, the waterlogging point disaster will be very serious. By using SWMM system simulation, we found that waterlogging disaster is not only related to extreme precipitation and regional distribution, topography, pipe network and runoff are also important factors leading to urban waterlogging.

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Ecological vulnerability evaluation of Yalong river basin

WANG Han, WU Liang-xu, SHAO Huai-yong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 84-88. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.019

Abstract （355）

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Based on GIS, evaluation indicators from terrain, meteorology, vegetation, soil, human activities, et al, were selected, spatial principal component analysis was used to quantitatively evaluate the local ecological vulnerability of Yalong river basin, and the spatial pattern characteristics and driving force of ecological vulnerability in 1995, 2005 and 2015 were explored. The results showed that,in terms of time scale, the comprehensive index of ecological vulnerability of Yalong river basin increased gradually, and the overall condition of ecological environment was at a medium level. In the perspective of spatial scale, the ecological vulnerability degree gradually reduced from upstream to downstream. In different altitude gradient zones, the ecological vulnerability degree of the extremely high altitude area （≥4 500 m）and the high altitude area （3 500~4 500 m）was relatively high, while the ecological vulnerability degree of the middle and high altitude area （2 500~3 500 m）, the middle altitude area （1 500~2 500 m） and the low altitude area （<1 500 m）was relatively weak. Owing to the vigorous implementation of ecological environment protection policies by the local government and the enhanced environmental protection awareness of local residents, the ecological environment of 9 counties in the study area improved from 2005.

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List of Chironomidae from Kuocangshan nature reserve, Xianju, Zhejiang province

SHI Sai-shuai, YIN Yi-qing, HU Zhen-zhu, LIN Li-yan, QI Xin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 89-91. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.020

Abstract （416）

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A list of Chironomid midges from Kuocangshan nature reserve, Xianju, Zhejiang province is given here. It includes 46 species, belonging to 23 genera and 4 subfamilies.

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Study on seed propagation technology of Valeriana officinalis

YANG Qiang, WEN Li-ming, ZHANG Ya-juan, JIANG Nian, LI Jiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 92-96. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.021

Abstract （426）

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Collecting seeds from Xianfeng county, Lichuan city, Enshi city in Hubei province, Guizhou province and Sichuan province, the soaking time, soaking water temperature, hormone treatment and cold storage treatment were studied in order to find out the best way of seed germination treatment and the difference of seed germination in different places. At the same time, the emergence rate of seeds in different areas was studied. Result showed that, there was no significant difference in the germination of V. officinalis seeds collected from five regions. The best way of seed germination was that the seeds were refrigerated for 28 d at -5 ℃ before sowing and then soaked in 0.08% GA solution at 50 ℃ for 16 h, with the germination rate of more than 30%. Under the best treatment conditions, four regions in Enshi prefecture （the altitude range was 580~1 436 m） on the basis of seed germination, the emergence rate was more than 40%. The seeds of V. officinalis germinated well under suitable conditions and could be widely promoted in Enshi prefecture.

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Effects of different control measures on physical and chemical properties of greenhouse soil and pepper growth

XIE Xin-yu, HUANG Xing-xue, ZHOU Guo-lin, YE An-hua, ZHANG Run-hua, DENG Yao-hua, LIN Jing, LI Jia-xing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 97-101. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.022

Abstract （321）

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In order to eliminate the negative effects of straw returning to the field and improve the soil properties, this study used pepper（Capsicum annuum L.） as a crop and facility continuous cropping soil as the treatment object, the experiment used no treatment as a control, and studied the high temperature stuffy shed, conventional strong reduction, strong reduction straw returning to the field, chemical effects of trichloronitromethane fumigation and flooding on physical and chemical properties of facility salinized soil and the growth of pepper. The results showed that, compared with other treatments, the strong reduction straw returning treatment reduced the soil EC value to the lowest, with less salt return. The effect of killing pathogenic bacteria is good, no chemicals are needed. The incidence of diseases is lower; the growth of pepper roots and plants is promoted. The yield of the pepper is the highest, the rate of missing plants is the lowest, and production safety is higher.

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Effects of different amount of nitrogen fertilizer on the growth of Cuiguan pear bud seedlings

XU Li-rong, YANG Fu-cheng, ZHU Bao-qing, ZHANG Hua-gai, GAO Zheng-gang, ZHANG Su-jie

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 102-104. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.023

Abstract （368）

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The effects of different amount of nitrogen fertilizer on the growth of Cuiguan pear bud seedlings were studied by a small amount of multiple fertilization method. The plant height, stem diameter and number of leaves of Cuiguan pear bud seedlings at different nitrogen levels were determined. The results showed that under high and medium nitrogen level, the plant height, stem diameter and leaves number during the six periods were the highest on the whole. According to various indexes, it is determined that the nitrogen fertilizer of per plant was 300 g, and the average annular ditch was divided into four times, which was most beneficial to the growth of Cuiguan pear bud seedlings.

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Urban riverside landscape planning research： Taking the planning of Xiaojiang of Dongchuan district in Kunming city as an example

REN Guang-zhen, SUN Li-qiang, TIAN Yu-hui, WANG Peng-fei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 105-108. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.024

Abstract （516）

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Based on the theory of landscape ecology and landscape architecture planning and design, the principle of urban banding riverside landscape space shaping was put forward, and the theory and approach of urban river ecological restoration and riverside landscape space planning were discussed by taking the urban section planning of Xiaojiang main stream in Dongchuan district of Kunming city as an example.

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Preliminary study on ecological landscape design of urban river

HU Bi-ying, YANG Hong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 109-112. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.025

Abstract （354）

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The general principles, technical methods and low impact development facilities of ecological landscape design were analyzed. On this basis, three design contents including landscape elements, water source updating, and road network improving were introduced, then the future development direction of urban river regulation combining landscape system construction and ecological sustainable development was put forward.

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The influence of plant communities on the birds communities in the eastern of Yulin Normal University

LIANG Yu, WEI Liu-feng, HUANG Ling-ling, BEI Yong-jian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 113-117. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.026

Abstract （320）

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From April 2015 to May 2016, the influence of plant communities on the bird communities was investigated through the line transect method and the point sampling method in the eastern of Yulin Normal University, the birds was surveyed two times a month. The results showed that, there was a total of 6 849 birds / times in the east campus of Yulin Normal University, and which belonged to 48 species, 8 order 23 families 33 genus, The mainly habitat types in the campus are Eucalyptus robusta Smith, Amygdalus communis L., A. persica L., Ficus concinna, F. benjamina L., Michelia alba DC., Lagerstroemia speciosa （L.）Pers, Melia azedarach Linn., Bombax malabaricum DC., the lawn, bush, the ground, and another. Among them, there are nine kinds of normal plants. It was concluded that, the most quantity of birds is Ficus concinna, Amygdalus persica, and Amygdalus communis. Comments and recommendations about the avian diversity in Yulin Normal University are provided based on the research results.

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Optimization of extraction conditions of organic acids from Prunus mume and its inhibition on biofilm formation

BO Xiao-wei, YANG Zhi-ping, QI Guo-hong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 118-122. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.027

Abstract （420）

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The extraction conditions of total organic acid from Prunus mume was optimized and its inhibition on biofilm formation by Staphylococcus aureus was studied. The single factor and orthogonal test were used to optimize and verify the total organic acid extraction scheme of Prunus mume. The amount of biofilm formation by Staphylococcus aureus was determined by crystal violet staining. The results showed that the optimum extraction conditions of total organic acids were ethanol 70% （volume fraction）, solid-liquid ratio of 3 g / 40 mL, ultrasonic extraction time of 1.5 h and ultrasonic extraction temperature of 50 ℃. The extraction rate of total organic acid was 24.50% from Prunus mume under these conditions. The inhibitory rates on biofilm formation by Staphylococcus aureus was 13.06%,48.24% and 74.01% respectively at the extract concentration of 0.5MIC, MIC and 2MIC. Therefore, the extract could inhibit the formation of Staphylococcus aureus biofilm.

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Study on extraction process of total flavonoids from Trifolium repens

WANG Jun-jie, MAI Kang-ming, WEN Xue-er, REN Peng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 123-126. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.028

Abstract （364）

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Based on the ultrasonic extraction method, the optimal technological conditions for extracting flavonoids from Trifolium repens was explored. With rutin as a blank control, the total flavonoid yield rate was an inspection index, and the effect of three factors, which were ultrasonic time, ethanol concentration and liquid-solid ratio, on the extraction rate of flavonoids in Trifolium repens was studied by the sodium nitrite-aluminum-sodium hydroxide method, single-factor test and L₉（3³） orthogonal test design. The results demonstrated that the optimal extraction process conditions of total flavonoids from Trifolium repens were ultrasonic time 40 min, ethanol concentration 50%, liquid-solid ratio of 60∶1（mL∶g）. Under these conditions, the extraction rate of total flavonoids in Trifolium repens was 2.853%. The test design method was highly efficient, fast and economical. It was suitable for the research on the extraction process of total flavonoids from Trifolium repens and provided experimental basis for the effective extraction of flavonoids from Trifolium repens.

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Extraction of total flavonoids and chlorogenic acid from Eucommia ulmoides Oliver in aqueous two-phase system

ZHANG Yi-lin, YAN Yong, ZHANG Qin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 127-131. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.029

Abstract （410）

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In this study, the two-phase extraction method was used to assist the extraction of total flavonoids and chlorogenic acid in Eucommia ulmoides Oliver. The optimal extraction process was determined by single factor and Box-Behnken response surface method test, and the total flavonoids and chlorogenic acid in Eucommia ulmoides extract were separated by ethanol/inorganic salt aqueous two-phase system. The results showed that under the optimal extraction conditions, the best aqueous two-phase extraction system for total flavonoids in Eucommia ulmoides was 50% ethanol content with 20% mass fraction of K₂HPO₄, the phase ratio R was 2.93 and the distribution ratio D was 10.35, and the extraction ratio could reach 77.94%. The best aqueous two-phase extraction system for chlorogenic acid was 60% ethanol with 20% K₂HPO₄in water, the phase ratio R was 3.43 and the distribution ratio D was 12.32 with the extraction ratio up to 78.22%. The dual aqueous phase extraction technology with mild development conditions, stable phase and short time is great significant for the development and utilization of Eucommia ulmoides.

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Analysis of volatile components of Aster tataricus L.f. and its honey-roasted products by HS-SPME-GC-MS

HAN Man, JIANG Han-mei, ZHENG Ying

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 132-136. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.030

Abstract （344）

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For the first time, using HS-SPME and GC-MS to extract and analyze the volatile constituents in Aster tataricus L.f. and its honey-roasted products. The results show that 85 peaks were detected from Asteris radix et rhizoma, 70 components were identified, accounting for 92.66% of volatile components; 75 peaks were detected from its honey-roasted products, 63 components were identified, accounting for 90.54% of volatile components. And there were 36 common components. The total ion flow diagram of their volatile components are somewhat similar, but their composition and content are different. At the same time, this experiment provides scientific basis for further development and utilization of Aster tataricus L.f. resources.

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Analysis of volatile components in Curculigo orchioides and wine-processed by HS-SPME-GC-MS

WANG Wen-tong, JIANG Han-mei, ZHANG Zhi-jing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 137-139. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.031

Abstract （408）

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Headspace solid phase microextraction （HS-SPME） combined with gas chromatography-mass spectrometry （GC-MS） was used to analyze the volatile components of Curculigo orchioides and wine-processed, and the relative contents of each component were calculated. The results showed that 22 peaks were isolated from the volatile components of Curculigo orchioides, and 16 components were identified, accounting for 72.73% of the volatile components, of which the highest was dextroterpene, accounting for 16.22% of the total volatile components. 28 peaks were isolated from the volatile components of Curculigo orchioides, and 24 components were identified, accounting for 85.71% of the volatile components, the highest of which was nonanal, accounting for 10.09% of the total volatile components. Part of the volatile components disappeared and some new volatile components were added after the production of Curculigo orchioides. It can provide scientific basis for the development, utilization and clinical application of Curculigo orchioides.

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Research on wood defect image reconstruction and quality evaluation model based on deep reinforcement learning

ZHANG Xu-zhong, ZHAI Dao-yuan, CHEN Jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 140-145. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.032

Abstract （373）

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Aiming at the problem of wood defect image perception and quality decision-making in typical bionic intelligent algorithm, the defect image distortion was serious, the variance of prior feature extraction of defect image fluctuates frequently, the gray level segmentation of defect image with uneven texture is invalid, the generalization ability and learning ability of different wood texture are unbalanced, and the optimal convergence speed is delayed with the defect dimension, a model of wood defect image reconstruction and quality evaluation based on deep reinforcement learning was proposed.By introducing the deep learning mechanism and using the deep residual network for iterative training, we can realize the real-time and efficient reconstruction of the multi-dimensional defect image of different wood, build a panoramic autonomous perception model for the fine segmentation and feature extraction of multi-dimensional defect of different wood, and build a large data level shared resource pool of wood defect features;By introducing reinforcement learning mechanism and using depth deterministic strategy gradient algorithm, a high-dimensional decision mapping among iterative updating of defect features, independent decision-making, panoramic visibility, depth prediction and wood quality evaluation was established, which realized the horizontal sharing integration of multi-dimensional difference wood defect image reconstruction and quality evaluation. Taking an economic forest in Nanhu forest farm area of Huzhou city, Zhejiang province as the evaluation carrier, the engineering application analysis of the model was carried out. The verification results showed that the model proposed in this paper can better realize the multi-dimensional defect perception and reconstruction of wood, the autonomous intelligent decision-making of global optimal quality evaluation, and has the obvious ability of sensing autonomy, reconstruction reproducibility, autonomous decision-making, model generalization, etc show superiority.

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Establishment of computational formula of height of suspended grid based on dimensional analysis

WEI Jing-jing, MU Zhen-wei, SUN Wen-bo, WANG Lei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 146-148. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.033

Abstract （367）

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As to the establishment of monolayer suspended grid in the stilling basin, the height of it has close connection with many factors, and under the condition of these influencing factors, it is difficult to quantify the influence law. Therefore, in this thesis, by means of π theorem of dimensional analysis, it has establishment on the height formula of suspended grid, puts forward and deduces 7 non-dimensional π theorems, and then, by means of nonlinear curve fitting, it can get the computational formula of height of suspended grid and predict the reserved test data, while the predicted value can be matched with the actual value within 10% error, so that it can get the conclusion that the computational formula of height of suspended grid is accurate to some extent, which can provide some references for practical engineering.

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The identification and sequence analysis of the core gene of Hippo pathway in wild silkworm

MENG Gang, PENG Yun-wu, WANG Rui-xian, CHU Qu, LIANG Jia-jun, LING Jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 149-153. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.034

Abstract （392）

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The Hippo signaling pathway plays a key role in animal growth and development.According to the known sequences, we used BLAST software to identify the sequence of Hippo pathway component gene in the transcriptome of wild silkworm （Bombyx Mandarina）. The protein molecular weight, isoelectric point, secondary structure, conserved domain and conserved element were also prospected and analyzed by ORFinder, web CD-search tool, Expasy-protparam, EMBL-EBI and MEME bioinformatics tools. The homologous sequence alignment and phylogeny were analyzed by Clustalx and MEGA 6.0 software. The homologous genes of salvador, warts, mats, yorkie and scalloped were identified in wild silkworm, and ORF frame and coding protein were predicted. The result showed that,Warts, Sav and Mats of wild silkworm shared up than 98% similarity with that of domestic silkworm, while Yki and Scalloped shared 89.02% and 79.13% identity respectively between wild and domestic silkworm. The WW2 domain of BmYKi were deleted, and one peptide were inserted in YAP-binding domain of BmSd, while Sd of wild silkworm missing the hinge region between nuclear localization signal motif and proline-rich motif. Yki and Sd could be clustered into subgroup according to species, families and genera, wild and domestic silkworm were clustered into the same branch,suggesting that the homologous proteins could play species-based functional specificity as well as functional conservation. This study could provide a theoretical basis for further study on the mechanism of Hippo pathway in wild silkworm.

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Identification and genome sequence analysis of pepper mild mottle virus isolate from Paris polyphylla var. yunnanensis

YANG Lin-yi, CHEN Lu, CHEN Ze-li, FEI Jin-qiang, WANG Zhe, XU Shao-zhong, ZHAO Ming-fu, WEN Guo-song

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 154-157. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.035

Abstract （430）

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Paris polyphylla var. yunnanensis is a perennial herb, and its medicinal part is underground rhizomes. It is an important chinese medicinal material. In order to clarify the viral pathogens infecting the Paris polyphylla, the transmission electron microscopy, DAS-ELISA and RT-PCR techniques were performed on the Paris polyphylla collected from Qujing, Yunnan province. Baculovirus particles were observed under transmission electron microscopy and were（200~300）nm ×（20~36）nm in size. The results of DAS-ELISA on 7 samples collected showed that 5 of them were positive. A whole genome sequence of a 6 357 bp Qujing isolate （PMMoV-QJ, accession number MK784568） was cloned by RT-PCR amplification. The homologous sequence of PMMoV-QJ were analyzed by homology with 10 PMMoV isolates reported at home and abroad, and the homology was between 99.42% and 99.81%. Phylogenetic analysis based on the whole gene sequence showed that PMMoV-QJ was closely related to Korean isolates. This study clarifies the genetic relationship of the isolate, and identifies the isolate from the molecular level, which provides a theoretical basis for the prevention of the subsequent viral disease.

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Research on the motivations and mechanisms of the evolution of Shanghai agricultural industrial system in the context of the construction of three modern agricultural systems

LIAO Pei-tian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 158-162. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.036

Abstract （347）

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In the context of rural revitalization strategy and the construction of modern agricultural industrial system, productive system and managerial system,this research studies the evolution motivation of Shanghai agricultural industry system, aiming to guide the construction of Shanghai urban modern agricultural industrial system, and to help other areas of China to build modern agricultural industrial system. Firstly, this research studies the evolution mechanism of agricultural industry system, and concludes that the evolution mechanism of agricultural industry system includes four aspects such as system guidance, demand pull, resource guidance and technology promotion. After that, the evolution of Shanghai agricultural industry system is divided into four periods such as rural agricultural industry system, suburban agricultural industry system, urban agricultural industry system and urban modern agricultural industry system. Complete data of Shanghai agriculture from 1995 to 2017, including per capita disposable income of rural residents, the yield ratio of food crop to cash crop, yield ratio of planting industry to breeding industry and ratio of added value of primary industry to GDP are used for analysis,the LSD method is used to check the phase difference among agricultural industrial systems,the test results are statistically significant. Finally, the research analyzes the evolutionary causes of each stage of Shanghai agricultural industrial system , and reveals the evolutionary process of Shanghai agricultural industrial system. It is found that the causes of the evolution of Shanghai agricultural industrial system have a great relationship with the function demand of agriculture in urban positioning,the agricultural industrial system in different stages realizes different dominant functions of agriculture. At present, the goal of constructing Shanghai modern urban agricultural industrial system is to realize the ecological function of agriculture.

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Study on the coupling and coordination of economy-tourism-environment in Guangdong province

MENG Bo-lin, MA Xiang-ping

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 163-169. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.037

Abstract （339）

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According to the facts of Guangdong province, establishing a coupling and coordinated evaluation index system for the economic, tourism and environmental systems of 21 prefecture-level cities in Guangdong province from 2008 to 2017. Calculating the weight of each index by using the entropy method , and evaluating the comprehensive development of each system. Analyzing the coupling coordination relationship of the economic-tourism-environment system by using the mathematical model and calculation method of coupling coordination degree. The results show that, 21 prefecture-level cities in Guangdong province have a high degree of economic-tourism-environment coupling, and there are no correspondence in spatial distribution between the coupling strength and the degree of coupling coordination; The economic-tourism-environment coordination degree in the Pearl River Delta region is relatively high, reaching a moderate and highly coordinated development level, and is an environment lagging type. The east-west wing and the northern Guangdong mountainous region's economic-tourism-environmental development is unbalanced, which is a lagging type of tourism and a lagging type of economy.

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Analysis of green water efficiency in Yangtze River Delta region based on DEA model

LUO Shi-chun, HUA Rui, SONG An-na

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 170-174. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.038

Abstract （370）

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The total water consumption, fixed assets investment, employment figure, amount of discharged wastewater and GDP output of three provinces and one city in the Yangtze River Delta from 2002 to 2017 were choosed to construct the DEA-Malmquist index model. The green water efficiency of the provinces was analysed,combining with environmental factors. The result showed that year 2010 and year 2015 were two obvious turning points of green water efficiency in three provinces and one city, which showed the significance of water conservation and water pollution governance policy implementation; During the study period, TFP total factor index was greater than 1, but technical efficiency showed a regressive trend, especially in Anhui Province. Therefore, the provinces should pay attention to optimizing industrial structure while implementing water-saving policies, ensuring technological progress and expanding industrial scale.

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Research on perception co-word of brand tourists in Shanxi, Shaanxi and Henan tourism regions based on grounded theory

ZHU Xiang-mei, WANG Jing, GAO Na, GUO Shao-hua, YAO Lu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 175-182. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.039

Abstract （346）

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Taking 12 tourist attractions in Shanxi, Shaanxi and Henan as research samples, this study abstracts and analyzes the key words of tourists online comments through grounded theory, calculates the comprehensive weight of key words combined with word frequency statistics and point praise analysis, constructs the co-word matrix and draws the co-word network by using Matlab and Netdraw software, and further analyzes the node characteristics and network structure of the network through UCINET software. Finally, SPSS software is used to cluster analysis of co-word matrix. It is concluded that the focus of tourists attention is the key factor affecting the image and satisfaction of scenic spots, mainly focusing on five key components, such as ecological environment, characteristic landscape, scenic spot management, humanistic flavor and tourism service facilities. At the same time, there are some differences in tourists perception in the three provinces of Shanxi, Shaanxi and Henan in five components.

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Study on intensive land use in urban development zone of Urumqi by AHP

WANG Yao, CAO Yue-e, LI Wan-nian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 183-188. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.040

Abstract （409）

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As an important growth pole of urban economy, the level of intensive land use is directly related to urban economy and regional land sustainable use. In this research, the intensive land use in Urumqi development zone from 1993 to 2016 was evaluated by literature review, causal analysis, time series analysis, and analytic hierarchy process （AHP）. The results showed that Urumqi high-tech industrial development zone has developed rapidly since it was approved by the state council in 1992. The score of land intensity has increased from 39.42 in 1993 to 97.18 in 2014 and then fell back to 93.51 in 2016. Residential land gradually becomes the main land use structure in urban development zones. At the development end of urban development zones, there existed some phenomena, such as urban expansion surrounding the development zone, enterprise capital precipitation and declined land use efficiency. It suggested that Urumqi high-tech industrial development zone should be optimized under the guidance of Urumqi's overall urban planning. The improving of land use efficiency should be simultaneously followed by high-technic upgrading in new energy and new materials, inner industrial land improving and further optimizing the land use structure in the development zone.

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International scientific and technological cooperation model under the background of innovation-driven and scientific and technological cooperation mode between China and Austria

LIN Xi-yan, CHEN Xiong, XIN Bing-qing, YU Sha, MA Yun-fei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 189-194. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.041

Abstract （400）

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Conducting international scientific and technological cooperation is internal need for countries around the world to enhance innovation capability, make up their weaknesses and achieve high-quality development. Combing 2015—2019 global innovation development report, summarizing the global pattern of scientific and technological innovation, the evolution trend of innovation law is as follows： from attaching importance to basic research expansion to scientific and technological innovation performance; from innovation quantity to innovation quality; global innovation shows a multipolar development trend; science and technology integration; innovation chain and industrial chain integration; promoting scientific and technological innovation through international cooperation. In this context, referring to the Pasteur quadrant model and combining with the reality of China's scientific and technological innovation, the international cooperation path of two-way integration of innovation chain and industrial chain is put forward, and the corresponding countermeasures and suggestions are proposed taking China-Austria scientific and technological cooperation as an example.

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Study on the withdrawal issue of public rental housing in Urumqi：Based on game theory

LIU Ying-zhen, HAN Fang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 195-198. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.042

Abstract （387）

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Public rental housing is an important housing security measure in China, which aims to solve the housing problem of low- and middle-income residents. However, the withdrawal of public rental housing is a great problem in the later manegement of public rental housing. In the more than 10 years since 2007, Urumqi had built more than 70 000 sets of public rental housing, which had greatly eased the housing problem of the low-income group in the capital, and played a positive role in the local social stability of Urumq. However, the withdrawal of public rental housing at the same time still needs to be paid more attention by policy makers and managers. It is urgent to study the problems such as inadequate management, imperfect mechanism and illegal occupation of public rental houses. This study takes five public rental housing communities in Urumqi as samples, uses Logistic model to analyze the game evolution of the lessee's rent-seeking behavior based on the factors affecting the lessee's rent-seeking behavior, so as to provide effective suggestions for the establishment of the flexible exit mechanism of public rental housing in Urumqi, so that the public rental housing policy can benefit a wider range and improve people's happiness in life.

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Research on the protection of Chinese traditional medicine Pinellia ternate for treating COVID-19

SUN Yuan-peng, LIU Yu-si, CHENG Zheng, SUN Yan-ling, WU Zhe

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 199-204. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.043

Abstract （630）

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Pinellia ternate is a kind of superior traditional Chinese medicine in the Central China geo-authentic crude drug producing area. It is one of 21 traditional Chinese medicines in Lung Cleansing and Detoxifying Decoction to treat COVID-19 （Corona Virus Disease 2019）. The medicinal value of Pinellia ternate, its GAP（Good Agriculture Practice）certification and its ecological suitable planting counties in the poor areas were introduced.The intellectual property resources involving the Chinese herbal medicine was summarized. Mainly based on agricultural intellectual property rights, advantage area of characteristic agricultural products, the construction of production base of genuine medicinal materials production base of genuine medicinal materials and or so, the strategies for protecting its geo-authenticity and promoting the high-quality development of Pinellia ternate were discussed under the rural revitalization strategy and the development strategy of traditional Chinese medicine.

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Urban integration and migrant workers’ choices of skill training：Based on different sizes cities investigation

LIU Yun-ru, QI Di-ming, ZHENG Xiao-yun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 205-211. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.044

Abstract （350）

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The types of skill training is divided into three parts such as rural return type, urban survival type and the urban development type. Viewing from the integration into urban society’s perspective to study the influence about migrant worker’s skill training choice, it turns out that the urban settlement will and the psychological identification are positively related to the will migrant worker’s skill training, compared with different sized cities, it has the same influential effect. To give a further investigate about the influence of the choices of migrant worker’s skill training related to merging into the urban community, the urban settlement will and the psychological identification are positively related to the will of migrant worker’s skill training, which means that the migrant workers who want to live in the cities or think they are urban prefer a higher-level in the training type choices. And it shows that only in medium-sized cities, merging into the urban community is positively related to the choices of migrant worker’s skill training, neither of the large citiesnor the small cities pass the significance testing. Therefore, we need to pay attention to the migrant workers of merging into the urban community during push the work of the migrant workers skill training. And it also require to put emphasis on individual city’s integration difference to achieve a differential, ladder skill training ,which can promote the human capital of the migrant workers.

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Research on the development strategy of cultural tourism industry cluster in Northern Shaanxi under the new normal economy：Taking the rural cultural tourism industry as an example

GUO Yue-nan, LYU Jian-yong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (13): 212-216. DOI: 10.14088/j.cnki.issn0439-8114.2020.13.045

Abstract （401）

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Industrial clusters have become the main phenomenon in the world economy at present, and have been highly valued by many countries. The necessity of the development of cultural tourism industry cluster under the new normal economy was analyzed, and the feasibility of the cultural tourism industry cluster spanning development in Northern Shaanxi was discussed. By taking rural culture tourism industry as an example to analyze the main paths of cluster development. The study aimed at making a deep analysis of the significance and strategy of the development of cultural tourism industry cluster under the new normal, and provided theoretical support for the innovation and development of cultural tourism industry in the region in Northern Shaanxi.

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Research and development of walnut diaphragma juglandis based on literature

ZHANG Feng, LYU Hong-lin, YANG Jing-juan, CHEN Chao-yin, NING De-lu, MA Ya-ge, ZHAO Sheng-lan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 5-11. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.001

Abstract （566）

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Based on the China Knowledge Network, Web of science, China Patent Announcement, and World Intellectual Property Office PATENTSCOPE database,the literature of walnut diaphragma juglandis were studied, and its the current research status was analyzed. The results showed thatdiaphragma juglandis was first recorded in 1841, and the journal literature first appeared in 1983. At present, there were 103 Chinese literature and 6 English literature. There were 237 Chinese patent applications, 16 authorized patents and 22 English patents. The patent literature first appeared in 2001, mainly involving composition （30 pieces）, tonifying Yang （28 pieces）, tonifying Qi （26 pieces）, and tonifying Yin （21 pieces）. It was known to contain mainly flavonoids （6.26%）, polysaccharides （5.4%）, total saponins （4.19%）, alkaloids （3.81%）, phenolic acids （3.39%）, volatile oils and other functional gredients. It had anti-tumor, anti-oxidation, anti-inflammatory, lowering blood sugar and other effects. The development of diaphragma juglandisbag beverage brewing process, health functional wine, instant tea and other aspectsneed further study.

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Effects of cultivation and management measures on soil organic carbon and nitrogen accumulation and crop yield in rice-wheat rotation system

JIN Zhao-qiang, NIE Li-xiao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 12-17. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.002

Abstract （407）

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Cultivation management measures will affect the accumulation of organic carbon and nitrogen nutrients in the soil of rice-wheat rotation system, and then affect the yield of rice and wheat. The latest research results in this field were reviewed, and the soil tillage, rice and wheat planting and water and nitrogen management methods which affected soil organic carbon and nitrogen accumulation and rice and wheat yield in rice-wheat rotation system were analyzed in detail,and the relevant suggestions for maintaining the soil fertility and increasing the yield of rice and wheat in rice-wheat rotation system were put forward, in order to provide certain theoretical guidance for rice and wheat production in our country.

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Analysis of biomass carbonization and conversion to fields as a technical approach to soil improvement and circular agriculture

WANG Li-yuan, LI Xiao-long, REN Tian-bao, XU Chen-sheng, LIU Ju-hua, ZHANG Yong-jiang, CHEN Nan, WAN Ying-fa, LIU Guo-shun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 18-23. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.003

Abstract （427）

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Circular agriculture and green low carbon is an important way of promoting the construction of ecological civilization. Agriculture to biomass returning carbonized has good development potential in soil improvement. The application of biomass charcoal in soil has an impact on the function of agricultural soil ecosystem by improving soil physical and chemical properties, improving soil fertility and remediation of soil pollution. Based on the physicochemical properties of biomass charcoal and the basic approach of carbonization returning to the field to improve the soil, the research status of biomass charcoal used in soil improvement was systematically analyzed, and the outstanding advantages of biomass charcoal in improving tobacco planting soil was identified. The utilization model of biomass carbonization technology was proposed, and the potential of biomass carbon in tobacco planting soil conservation and nutrition management was discussed.

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Dynamic characteristics and trend prediction of ecological carrying capacity in Dongting Lake area

TAN Jie, HAO Qi, TAN Xue-lan, WU Yi-ze, ZHOU Wei-jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 24-29. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.004

Abstract （387）

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The state space method was used to evaluate the evolution of ecological carrying capacity in Dongting Lake area in 2005, 2010 and 2015. The ecological carrying capacity of Dongting Lake area in 2020 and 2025 was simulated and predicted by grey prediction model. The results showed that the ecological carrying capacity of Dongting Lake area exhibited an upward trend from 2005 to 2015. In addition to the carrying capacity of resources and environment, the ecological resilience and social and economic coordination were in a state of overload. From 2020 to 2025, the ecological carrying capacity of Dongting Lake area was expected to continue to increase and remain in the loadable state. Stability and coordination degree of the complex natural, economic and social dynamic system in the area will rise to some extent. The ecological resilience and the coordination of social economy were the main restricting factors for the improvement of ecological carrying capacity. In the future, Dongting Lake area should further transform the economic development mode and strive to improve the ability of ecological system self-regulation and self-maintenance.

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Study on cultivated land pattern and landscape fragmentation in Qianjiang city

XU Li-yang, MARHABA·YUSUFU

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 30-36. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.005

Abstract （413）

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Based on the land cover data of GlobeLand 30 in 2000 and 2010, taking Qianjiang in Hubei province as the research area,the soil spatial and temporal variation characteristics of cultivated land in Qianjiang is analyzed. And the arable land patch area and the fragmentation index of 19 towns in the city are calculated. The cultivated land area and the fragmentation coupling mode of 9 kinds of cultivated land change are established. The changes in cultivated land, cultivated land fragmentation country towns and the characteristics of both associated with formation of the farmland change are analyzed. The results show that the total cultivated land area of Qianjiang remained stable from 2000 to 2010, but the variation range of the cultivated land area of each town and town within Qianjiang was significant, and the cultivated land fragmentation was intensified. The cultivated land change pattern of “the reduction of cultivated land area with the increase of fragmentation” is dominant in Qianjiang, and it is located in the northeast region where urbanization is developing rapidly. The townships with the pattern of “increase in cultivated land area with reduced fragmentation” are mainly distributed in the southwestern regions where agriculture is relatively developed. The urban construction land gradually occupied the cultivated land in Qianjiang, resulting in a sharp decrease in the area of cultivated land at the edge of the city, and the internal fragmentation trend of the cultivated land is serious.

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Study on cleaning and quality control technology of agricultural micro-meteorological observation data

ZHOU Qiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 37-40. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.006

Abstract （389）

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In order to improve the quality of agrometeorological observation data, the cleaning standard and quality control method of agrometeorological data are established based on the unique meteorological observation equipment and environmental attributes of farmland. For the case of abnormal data attributes and repeated records, the method of cleaning the blank value of Bohn data cleaning model and the method of cleaning the noise data are selected. Through the historical data statistics of observation elements in the space of agricultural micro meteorological observation station, the clean data index is obtained and applied to the dynamic threshold generation method of data quality, and the quality control model of agricultural micro meteorological data is established. The data evaluation indexes after cleaning and quality control showed that the accuracy and repeatability of the data are significantly improved after data cleaning and quality control model. The data cleaning quality control method is helpful to obtain the monitoring information of agrometeorological disaster accurately and provide effective decision support for agricultural disaster prevention and reduction.

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Research and application demonstration of key technology of lightning disaster prevention in rural areas of Hunan province

LIU Feng-jiao, WAN Xie-cheng, YANG Jia-yan, HE Qiu-yan, LIU Yan-qing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 41-44. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.007

Abstract （400）

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To improve the level of rural lightning disaster prevention science and technology as the goal, the lightning disasters were analysed in Hunan province. Key technical indexes and comprehensive technical methods for rural lightning disaster prevention had been established. National mandatory standards had been developed and issued. And the application and demonstration of comprehensive lightning disaster prevention technology had been successfully carried out. The following innovative results were obtained： ①The national standard for the lower limit of lightning strike frequency of rural residential lightning protection buildings and the corresponding key points of design and construction technology were determined; ②The symmetrical unequal spacing quadrupole method was proposed as the preferred method to measure the soil resistivity and a patent was applied, which can better reflect the actual stratification of natural soil and was more stable and more accurate; ③The distribution map of soil resistivity in Changsha region was drawn, and the analysis software was developed. It was found that the soil resistivity value was significantly positively correlated with precipitation; ④Integrated lightning protection facilities construction technologies, such as simulation trees, emergency shelters and single rural residential lightning protection facilities were used to create a regional protection system for thunderstorm path interception, and a new all-round and three-dimensional regional lightning protection and disaster reduction model in rural Hunan province was established.

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Analysis of the periodic change and trend of agro-climatic resources in Bei'an city based on Morlet wavelet

XU Ming-yuan, WANG Hai-ning, XU Wen-long

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 45-51. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.008

Abstract （430）

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Based on the 1959—2019 meteorological data of the national basic meteorological stations, farm meteorological stations and regional meteorological stations in Bei'an city, Heilongjiang province, using Matlab to make wavelet analysis chart for cycle analysis, using one-dimensional linear fitting and quadratic polynomial fitting for linear trend analysis, the agricultural climate resources affecting crop growth in Bei'an city for nearly 61 years were analyzed. The results showed that the temperature fluctuation in the growing season of crops in Bei'an city had shown an overall rising trend in recent 61 years, while the accumulated temperature had shown a significant upward trend, rising slowly at the rate of 70 （℃·d） every 10 years, which had been at a higher level since 2000,and the accumulated temperature increase made the heat resources showing an overall increasing trend. The precipitation showed no significant decreasing trend. The wavelet analysis showed periodic changes in monthly and total precipitation of the growing season, and the sunshine hours showed periodic changes, and the linear trend slightly decreased. The change trend of the first frost day was delayed year by year, and the change trend of the final frost day was advanced year by year. The number of frost-free days showed periodic changes, and the overall trend showed an increasing trend.

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Connection between precipitation of low latitude plateau in dry season and Indian Burma trough

DENG Jian-bo, LIU Kui, TANG Xiao-nan, ZHOU Bi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 52-55. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.009

Abstract （421）

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Based on monthly atmospheric circulation reanalysis data from Global Modeling and Assimilation Office（GMAO）and Goddard Earth Sciences Data and Information Sciences Center（GES DISC） and precipitation of 148 weather stations in low latitude plateau region from 1979—2012, the relationship between the India Burma trough and the precipitation of low latitude plateau in dry season was investigated by using correlation and synthesis method. The results showed that the India Burma trough was an important factor affecting the precipitation of low latitude plateau in dry season. Water vapor was transported to the low latitude plateau region by southerly wind in front of the India Burma trough. Strong vertical ascending motion in front of the trough promoted the CISK physic process and caused rainfall when met the cold and dry northerlies in the southeast of the Tibet Plateau. The positive feedback mechanism of atmospheric barocline development and radial circulation which would hamper the dispersion process of atmospheric barocline were established when the trough grows strong. As a result, the configuration of the meteorological filed can be affected, which further influenced the precipitation of this region in dry season.

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Spatial and temporal distribution characteristics of drought and its relationship with land types in Hengshao basin

ZHOU Li, ZHOU Hui, LI Qiao-yuan, ZHU Xin-wei, JIANG Shuai, YIN Yi-wen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 56-62. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.010

Abstract （370）

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Based on the conventional meteorological data of 97 meteorological stations in Hunan province from 1975 to 2015, the precipitation and drought characteristics in Hunan province were analyzed. On this basis, combined with the topography of Hengshao basin, the spatial and temporal distribution characteristics of drought in Hengshao basin from 2005 to 2015 were analyzed, and the relationship between drought and land use in this period was mainly studied. The results showed that the annual precipitation in Hunan province was different. The spatial and temporal distribution was uneven. The precipitation in most areas was mainly concentrated in April to June, accounting for 42% of the total precipitation in the whole year. The annual precipitation varied greatly in regional distribution. The precipitation in the west, south and east mountainous areas was more than that in the middle hills and the northern Dongting lake plain. The drought days in summer and autumn account for 60%~80% of the total number of drought days in Hunan province, especially in Hengshao basin in central Hunan province, where the frequency of drought was the highest, reaching more than 70%, and the spatial and temporal distribution of drought varies greatly among counties and cities in the region. Because of the influence of topographic subsidence airflow, the process rainfall in Hengshao basin was often less than that in other areas, and the probability of drought was higher than that in other areas. For most regions, the percentage of cultivated land, forest garden land and grassland was negatively correlated with composite meteorological drought index （CI）, while the percentage of urban and rural, industrial and mining, residential land is positively correlated with CI.

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Analysis of rural residential area patterns in oasis in extremely arid areas：A case study of Pishan county oasis

XU Cai-fang, CAO Yue-e, XU Zhong-lin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 63-68. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.011

Abstract （347）

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Based on the 2016 Land Change Survey Database to extract the spatial distribution information of residential areas, combined with qualitative and quantitative analysis methods, GIS spatial analysis, landscape pattern index and other methods, with the “polarization effect” and “hydrophilic effect” as an opportunity,the spatial heterogeneity of rural settlements distribution in Pishan county oasis in extremely arid areas is analyzed. The research shows that the residential areas in the study area are concentrated in the hinterland of the township center. With the increase of the radiation radius, the scale of the residential area gradually decreases, and the spatial agglomeration is more obvious. The HH agglomeration phenomenon appears in the hinterland of the cluster-type township center. With the increase of the radiation radius, the clustering of HL and LH outliers is gradually presented, and the distribution of linear residential sites is mostly random. The closer the layout of the residential areas closer to the administrative center is, the more regular, centralized, and the more spatially connected, the more obvious the “polarization effect”. With the increase of radiation radius of river water source, the residential areas in the study area are gradually concentrated and complicated, and the spatial connection degree of residential areas is gradually increasing; The dispersion of linear residential area is gradually decreasing, and the area disparity is gradually increasing; The degree of dispersion of shaped residential area gradually increases, and the area disparity increases gradually.

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Preparation of fungus based on garden waste and its application

PENG Chun-yan, GAO Yu-hui, WU Hua-nan, ZHENG Wei-guo, LIU Tian-xiang, LI Jun-juan, YU Dong, ZHENG Wen-fang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 69-75. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.012

Abstract （297）

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Based on the concept of “using waste to control pollution”, a fungus with high pollution resistance prepared from garden waste was applied to polluted river water to realize the linkage treatment of solid waste and waste water. Garden waste content, substrate thickness and initial moisture content were taken as influencing factors. The effect of the content, substrate thickness and initial water content of garden waste on the preparation of spores was investigated, and the preparation process was optimized by response surface method. The results showed that the most influential factor on the yield of spore was substrate thickness, followed by garden waste content and initial moisture content. The optimum conditions were garden waste content 46%, substrate thickness 3 cm and initial moisture content 57%. Under such conditions, the actual results were close to the predicted values of regression equation. Then the prepared spores were activated and added to polluted river water at an inoculum of 0.50‰. After 54 hours of aeration, the removal rates of COD, NH₃-N and TP reached 84.97%, 97.07% and 51.64%, respectively. Continuous experiments demonstrated that this integrated system could effectively remove pollutants such as COD, NH₃-N and TP in polluted river water with high stability.

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Study on the genus Harnischia, Kieffer from Zhejiang province

YANG Kai-nan, LIN Li-yan, YIN Yi-qing, CHEN Xiao-yu, QI Xin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 76-79. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.013

Abstract （352）

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Four species of the genus Harnischia Kieffer including Harnischia curtilamellata （Malloch, 1915）, Harnischia japonica （Hashimoto, 1984）, Harnischia tenuitubercula （Chaudhuri & Chattopadhyay, 1990）, Harnischia turgidula （Wang & Zheng, 1993） from Zhejiang province were described in detail. The main feature map and the species retrieve the table were attached. All the above four species were new species recorded in Zhejiang province, while Harnischia tenuitubercula （Chaudhuri & Chattopadhyay） was the first record for China.

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Effect and safety of nicosulfuron·mesotrione·atrazine and S-metolachlor in controlling spring corn weeds

LONG Shui-liang, WU Xiao-feng, LEI Gan-nong, KONG Xuan-qing, JIN Chen-zhong, LI Cheng-ye, OU Xiao-ming, TANG Juan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 80-84. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.014

Abstract （858）

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Using the closed weeding method before single seedling and the single weeding method after seedling in spring maize fields as control, the effects of the closed weeding method before seedling combined with the application method after seedling on Alternanthera Philoxeroides （Mart. Griseb.）, Chenopodium album L. and Artemisia lavandulaefolia DC in the maize fields of Yongzhou city, Hunan province were investigated. The results showed that the pre-emergence spraying S-metolachlor 1 200~1 800 g/hm², combined with post-emergence spraying 28% of nicosulfuron·mesotrione·atrazine 2 100~3 000 g/hm², the three kinds of weeds in seedling after applying pesticide, herbicide, the control effect and fresh weight of 55 d were over 90%, the overall higher than that of individual treatment and long-term effectiveness and safety.

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Explore the feasibility of breeding Arma chinensis with Tenebrio molitor as food

XIE Xiao-fei, HUANG Yong, XIA Peng-liang, REN Xiao-hong, WANG Rui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 85-87. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.015

Abstract （374）

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In order to explore the feasibility of breeding Arma chinensis with Tenebrio molitor as food, Arma chinensis’s developmental duration, mortality, adult weight and egg production by feeding on Tenebrio molitor pupae, Mythimna separata pupae and Mythimna separata nymph in the laboratory were measured and compared. The results showed that Arma chinensis feeding on Tenebrio molitor pupae could normally complete generation development, and there was no significant difference in body weight of adult Arma chinensis feeding on three kinds of food. Arma chinensis feeding on Tenebrio molitor： The nymph duration was 27.97 d, which was significantly lower than 25.30 d in the pupa group and 31.27 d in the larva group of Mythimna separata; The mortality rate of nymph was 26.67%, which was significantly lower than 33.33% and 83.33% of pupae and larvae feeding on Mythimna separata; The average spawning amount of single females in the generation was 266.67, which was significantly different from 413.00 in the pupa group of Mythimna separata and not significantly different from 292.3 in the larvae group of Mythimna separata.

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Sensibility of Echinochloa grass to quinclorac in the rice planting areas in Sichuan province

ZHU Jian-yi, ZHENG Shi-jun, PU Po, ZHAO Hao-yu, LIU Sheng-nan, YANG Xiao-rong, XIANG Yun-jia, ZHOU Xiao-gang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 88-90. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.016

Abstract （364）

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In order to understand the resistance of barnyard Echinochloa grass to quinclorac in rice fields of Sichuan province. Whole plant bioassay was conducted to determine the sensibility of twenty barnyard grass populations to quinclorac and to quantify their resistance level. The results showed that ten populations were still in a sensitive or sensitive decline stage. Nine populations produced different degrees of resistance to quinclorac, of which six populations were low resistant and three populations were medium resistant. Nearly 50% of the tested barnyard grass populations were resistant to quinclorac, most of which were low resistant or medium resistant. The resistance level was generally low.

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Evaluation of application effect of solar insecticidal lamp in rice field experiment

LIU Hong-ju, ZHANG Zheng-hong, CHEN Ai-fang, PU Zhi-ping, SUN Lei, YANG Zhi-yuan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 91-93. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.017

Abstract （356）

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In order to objectively evaluate the trapping and killing effect of the automatic insect-killing frequency vibration type solar insecticidal lamp on the main pests of rice, a comparative field experiment is carried out between the Bion automatic insect-killing frequency vibration type solar insecticidal lamp and the common frequency vibration type solar insecticidal lamp. The results showed that the control effect of Bion's automatic insect-clearing frequency-vibration insecticidal lamp on main rice pests is better than that of ordinary frequency-vibration solar insecticidal lamp.

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Effects of 5-aminolevulinic acid on yield and quality of cream cabbage

MA Jing-shu, ZHANG Qian-ru, SONG Jiao, ZHANG Mei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 94-96. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.018

Abstract （379）

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The effects of foliar application of 5-aminolevulinic acid at different concentrations on yield, chlorophyll, vitamin C, protein and soluble sugar content of cream cabbage were studied. The results showed that compared with the control group, spraying 5-aminolevulinic acid solution at different concentrations on the leaves had significant effects on the quality indexes of cream cabbage. The chlorophyll content and vitamin C content reached the highest when spraying 5-aminolevulinic acid solution at 1.70 g/L on the leaves, and the protein content and soluble sugar content reached the highest when spraying 5-aminolevulinic acid solution at 0.85 g/L on the leaves. The effect of spraying different concentrations of 5-aminolevulinic acid on the yield of cream cream was not obvious.

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Study on cadmium accumulation characteristics of different eggplant materials

LI Zhi-rong, LU Rong-hai, PAN Shao-kun, YANG Liu, LIN Li-jin, LI Hong-yan, XIANG Juan, WU Yong-mei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 97-100. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.019

Abstract （370）

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Twelve eggplant （Solanum melongena L.） materials were planted in cadmium-contaminated soil by pot experiment to study the biomass and cadmium （Cd） accumulation characteristics at the seedling and flowering stages. The results showed that there were obvious differences between eggplant materials on biomass and Cd content. The biomass in root, stem, leaf and shoot of the material XN-Z1-2 were all higher, while that of the material “43-1” was relatively lower;the Cd content of different organs of material XN-Z1-2 was lower but that of the material “43-1” was all the highest when eggplant was at the seedling stage. Overall, the biomass of each organ of the material “96-2” was higher and that of the material “43-1” was the lowest; the Cd content was the highest in various organs of the material “43-1” and generally lower in the material ZF-Z1-2 if eggplant was in the flowering stage. In conclusion, “43-1” was a kind of eggplant material with high Cd accumulation; the material XN-Z1-2 and ZF-Z1-2, all possessed higher biomass and lower Cd accumulation, were eggplant materials with low Cd accumulation which could grow normally in Cd-contaminated soil.

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Flowering stage and biological characteristics of different peony cultivars introduced in Wuhan city

HAN Yan-ni, LIU Yan-ling

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 101-107. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.020

Abstract （421）

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In order to understand the growth and flowering status of different peony varieties （Paeonia suffruticosa Andr.） combinations in Wuhan area, the flowering period and biological characteristics of five introduced peony varieties （Zhongyuan, Jiangnan, Japan, the United States and Francepeony cultivar group） in Wuhan were observed and studied from 2017 to 2019. Results showed that the flowering period of different peony cultivarswas mainly concentrated in April in Wuhan area,and the peak flowering was early and middle of April. There were also some differences in the flowering period of different peony cultivars in the introduced land. In addition, both planting foreign peony cultivars and domestic peony cultivars in the same garden could extend the flowering period by 10~15 days, and at the same time enriched the color of peonygarden. Compared with the place of origin, the peony color basically maintained the original color, but the flower shape was degraded. There were 44 % of foreign peony cultivarswhose flower shape showed degradation to different extents.

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Effects of foliar spraying of different regulators on quality of tobacco leaves

DONG Kun-le, MAO Jia-wei, KONG De-hui, ZHANG Xiang, ZHAO Hao-bin, HE Lei, LI Liang, SI Xian-zong, SUO Yan-yan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 108-111. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.021

Abstract （408）

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In order to improve tobacco quality, the comparative test was conducted in Luoning county and Biyang county of Henan province to study the effects of foliar spraying of different regulators （chitosan oligosaccharide, xylo-oligosaccharide, potassium dihydrogen phosphate, potassium nitrate, fulvic acid potassium salt） on quality of tobacco leaves. The results showed that, compared with CK, except treatment T6（fulvic acid potassium salt） in Luoning county, foliar spraying of different regulators promoted leaf development and increased leaf area. Compared with CK in both testing points, foliar spraying of different regulators increased the yield but the differences were not significant. The output value of Luoning county and Biyang county increased by 2.4%~20.2% and 6.6%~20.6%,respectively. The average price and high grade leaf proportion of treatment T4（potassium dihydrogen phosphate） were highest in the two testing points. Compared with CK, the potassium content of middle leaves with regulator application were increased and the potassium content of treatment T4 were highest in the two testing points. The nicotine and chlorine content of regulator application treatments decreased while the potassium content increased. The potassium content of cured leaves with treatment T3（xylo-oligosaccharides） were highest in the two testing points and the potassium content were 1.99% and 2.15%, respectively. Foliar spraying of different regulators could increase leaf area, yield, output value and quality of tobacco leaves. Among different treatments, treatment T3 and treatment T4 were better.

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Investigation on the quality of irrigation water in southern Anhui and the influence of metal elements in irrigation water on burned sugar aroma style of tobacco leaves

DONG Xiang-zhou, CHEN Ya-kui, XU Can-ran, ZHU Qi-fa, HUANG Wei-wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 112-116. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.022

Abstract （394）

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53 representative areas for tobacco planting in southern Anhui province were selected and the quality of irrigation water, especially the content of metal elements, was investigated. It was found that the content of micro （medium） elements in irrigation water was too low to have a significant effect on the formation of burned sugar aroma style of tobacco leaves. The contents of Mg, Ca and Zn were 0.7~8.0 mg/L, <40 mg/L and 0.002~0.029 mg/L, respectively. The heavy metal content of irrigation water and other basic control items all met corresponding national standards. Furthermore, the experiments on tobacco planting under controlled irrigation using paddy soil in a greenhouse proved that Zn was a negative correlation factor for forming the burned sugar aroma style of tobacco and the threshold value was ≥10 mg/L in irrigation water. Meanwhile, Mg was a positive correlation factor and the content of Mg to promote the burned sugar aroma style should be maintained at 40~90 mg/L. Ca and Mg had synergistic effects, but which was mainly appropriate for acid paddy soils.

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Reflections on the high quality development of Xiangyang high fragrant tea industry

LUO Jing-dong, TANG Qian-yong, CHENG Yi-fang, YANG Xiao-juan, YANG Wei, FENG Peng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 117-120. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.023

Abstract （344）

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The tea production in Xiangyang area has a long history, the tea culture is profound,and the brand influence is constantly enhanced. The advantages, development and problems of the high fragrant tea industry in Xiangyang city were reviewed and the solution countermeasures were proposed pertinently. There were also some unfavorable factors that restricted the development of the high fragrant tea industry such as the low degree of clone breeding, low management level and low processing technology, and the service system was not perfect. It was recommended to find a breakthrough, optimize the structural layout, strengthen talent construction and financial support, concentrate regional brand building, and promote the high-quality and healthy development of the tea industry.

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Study on seed breeding and seed growth law of leaf vegetable of Toona sinensis

LI Yue-feng, ZHANG Hong-wei, DONG Hui-hui, ZHANG Ying-zi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 121-124. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.024

Abstract （397）

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Based on analysis of annual seedling growth experiment of Toona sinensis leaf forest, the Logistic growth curve equation was used to analyze growth law at seedling stage. According to the growth rate of seedling height and ground diameter, the growth of Toona sinensis was divided into four different growth stages, emergence stage, early growth stage, peak growth stage and late growth stage. The results showed that the growth pattern of Toona sinensis leaf at seedling stage was“slow-fast-slow”. The growth was centered on the peak stage. The growth of seedling height accounted for 70.48% of the total growth and the ground diameter accounted for 78.05%.

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Fatty acid composition and feeding habit of fish in the Pearl river from Guangzhou to Humen

ZHANG Ting, XIANG Gang, LONG Sheng-xing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 125-132. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.025

Abstract （414）

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Fish are important consumers in aquatic ecosystem. Different of fish species were collected form the Pearl river, and the fatty acid content of fish were determined in order to understand characteristic of feeding habits. The results showed that Tilapia zillii, Cirrhinus molitorella, Hypostomus plecostomus, Cyprinus carpio, Hypophthalmichthys molitrix, Squliobarbus curriculus, Carassius auratus, Parabramis pekinensis, Odontamblyopus rubicundus, Chaeturichthys stigmatias, Coilia grayi and Lateolabrax maculatus are main common species in the river. The main fatty acids in fish including saturated fatty acids C14∶0、C15∶0、C16∶0、C17∶0、C18∶0, monounsaturated fatty acids C16∶1ω7、C18∶1ω9、C20∶1ω11, polyunsaturated fatty acids C18∶2ω6、C16∶3ω3、C20∶2ω6、C20∶4ω6、C20∶5ω3和C22∶6ω3. Saturated fatty acids concentration in fish changed from 1 001.49 to 97 634.47 μg/g, with average 33 044.02 μg/g, monounsaturated fatty acids concentration in fish changed from 1 161.09~170 924.8 μg/g, with average 33 044.02 μg/g, and polysaturated fatty acids concentration in fish changed from 859.99~98 269.52 μg/g, with average 23 846.78 μg/g. Fatty acid biomarkers showed that Clarias fuscus, Erythroculter pseudobrevicauda, Clarias lazera, Channa maculate, Channa asiatica, Collichthys lucidus, Lateolabrax maculatus, Cynoglossus joyneri, Squliobarbus curriculus, Carassius auratus, Aristichthys nobilis, Coilia grayi, Mugil cephalus, Anguilla japonica, Hypophthalmichthys molitrix, Misgurnus anguillicaudatus, Tilapia zilliimainly feed on attachments, benthic organisms, zooplankton and phytoplankton, and their carnivorous characteristics increase with body weight increasing.

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Annual observation on growth and life habit of adult Andrias davidianus in Qiannan region

CHEN Lin, XIONG Hong-lin, YU Zhi-wen, WEN Di, HUANG Xiao-na

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 133-136. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.026

Abstract （487）

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The feeding experiment on AdultsAndrias davidianusin Qiannan region lasted for 14 months and the results were as follows： Growth of body length and one of body mass were both different procedures and the obvious reverse relation existed in the both procedures. Positive relation existed between growth of body length and water temperature. The relation between growth of body mass and water temperature was complex and this kind of growth was confined by high and low temperature. Andrias davidianus liked dark and quiet conditions and had a wide diet. The more it defecated, the more it ate. The faeces looked like masslike or sandlike. Andrias davidianus had the habit of molting and the relation was positive between molting frequency and growth of body mass. The hibernation of Andrias davidianus was seldom observed indoors in Qiannan region and the increasing rate of weight could be effectively promoted by feeding enough bait in the peak growth period. The economic benefits of artificial breedingcould be significantly increased by feeding live carps fed by compound feedwith Pomacea canaliculata.

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Effects of photoperiods on activity rhythm of Pteromys volans

TIAN Xin-min, SHA Si-tong, YANG Meng-ping, LIAN Ming-dong, SHI Lan-ying

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 137-140. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.027

Abstract （425）

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The study was about the effects of photoperiods changes on the activity of the flying squirrel in autumn and winter. We studied by using infrared camera video recording and statistical behavior under constant temperature captivity in the laboratory. The result showed that the rhythm of the activity outside the nest of the flying squirrel showed seasonal difference, the rhythm of the activity was affected by the change of photoperiods, and it showed the endogenous rhythm characteristics of the species adapting to the field environment. In autumn and winter, the total activities, feeding and other activities outside the nest of the flying squirrel were trimodal, and affected by the photoperiod, the start and end of winter activity of the flying squirrel were delayed by about 1 h. Among them, the total activities outside the nest and feeding activities in winter were significantly lower than those in autumn. Through analysis, it was concluded that in the cold season （winter）, the flying squirrel still maintains the endogenous characteristics of reducing single activity time and increasing activity frequency in the field, thus reducing energy consumption.

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Study on development and stability of walnut and peanut compound milk beverage

HAN Xiao-jiang, GUO Yao-dong, WANG Tao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 141-144. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.028

Abstract （448）

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Using walnut, peanut and skim milk powder as raw materials to research and develop compound beverages, we firstly used the sensory score as the evaluation index by single factor test on the amount of walnut juice added, peanut milk addition, milk addition and sucrose addition. On this basis, three levels of orthogonal optimization and variance analysis were selected. The results showed that the addition of walnut juice was 15%, the amount of peanut milk added was 25%, the amount of milk added was 30%, and the amount of sucrose was 6%. The beverage tastes best and the flavor was good. Under the optimal conditions, sodium alginate, gum arabic and CMC were selected as stabilizers to investigate the stability of the composite beverage. The single factor test and response surface optimization showed that the amount of sodium alginate was 0.024% and the amount of gum arabic was 0.015%, CMC was 0.028%, the precipitation rate reduced to 0.415%.

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Study on extraction technology of total flavonoids from Chinese medicine Excoecaria cochinchinensis Lour.

HAN Qian, WEI Jiang-cun, CHEN Yong, LU Jing-rong, LI Jin-zhou, CHEN Zi-jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 145-148. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.029

Abstract （396）

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The total flavonoids of Excoecaria cochinchinensis Lour. were extracted by reflux extraction method. The effects of extraction method, extraction solvent volume fraction, extraction volume and extraction time on the total flavonoid extraction rate of the medicinal materials were investigated by single factor experiment. The orthogonal test was used to optimize the extraction conditions. The best extraction process for a total flavonoid of Excoecaria cochinchinensis Lour. was screened out. The result showed that the optimal extraction process of a total flavonoid of Excoecaria cochinchinensis Lour. was 70% methanol, the ratio of material to liquid was 1∶40（g∶mL）, and the reflux extraction time was 1.0 h. This method can effectively determine the content of total flavonoids in Excoecaria cochinchinensis Lour. The repeatability was good, the method was stable and feasible.

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Comparison of the sunscreen effect of 18 edible and medicinal plants

WEN Yan, WANG Xing, GAO Pan-qi, XU Jian-rong, CHEN Hong-ying

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 149-152. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.030

Abstract （477）

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In order to screen the natural raw materials of sunscreen, 18 natural medicine plants were selected for sun protection analysis. The antioxidant capacity, ultraviolet light absorption capacity and tyrosinase inhibition activities of the materials were compared. The results showed that Rosae rugosae flos, Eugenia caryophyllata Thunb., Ilex latifolia Thunb. and green tea had strong scavenging activity of free radical. The IC₅₀ of their extracting solution were 35.00, 65.00, 60.00 and 42.00 μg/mL, respectively. When the concentration of ethanol extracting solution was 0.2 mg/mL, Pueraria lobatae radix., Rosae rugosae flos, Coptis chinensis Franch., Ilex latifolia Thunb. and Eugenia caryophyllata Thunb. had a larger absorption value in the ultraviolet medium wave region, and the absorption value decreased successively. The absorption value of Coptis chinensis Franch., Ilex latifolia Thunb., Curcuma Longa Rhizoma., Rosae rugosae flos, Reynoutria japonica Houtt., Sophora japonica L. and Eugenia caryophyllata Thunb in the ultraviolet long wave region were relatively large, and the absorption was decreased successively. The average inhibition rate of Polygoni cuspidati rhizoma, Reynoutria japonica Houtt., Rhodiolae crenulatae radix, green tea, Zanthoxyli pericarpimum, the seed of Vitis vinifera L., Rosae rugosae flos, Sophora japonica L. and Ilex latifolia Thunb. on tyrosinase activity decreased in turn.

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Nutrient composition analysis of five kinds of wild edible ferns in Yunnan

YANG Xi, YUE Jian-wei, SU Cui, MENG Zhen-cheng, CHEN Hong-jie

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 153-155. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.031

Abstract （464）

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The components of 5 species of wild edible ferns in southeast Yunnan were analyzed： Callipteris esculenta, Pteridium aquilinum, Allantodia dilatata, Pteridium revolutum and Tectaria decurrens. The results showed that the contents of protein, soluble sugar, crude fiber and crude fat were 20.04%~32.72%, 1.24%~12.63%, 11.26%~24.68% and 0.54%~1.05% respectively. The content of potassium was 3 163~4 610 mg/100 g, the content of magnesium was 316~377 mg/100 g, the content of calcium was 125~350 mg/100 g, the content of iron was 5.589~8.309 mg/100 g, and the content of manganese was 3.578~10.420 mg/100 g. The content of protein, crude fiber, potassium, magnesium and manganese was much higher than that of green leafy vegetables, while the content of calcium and iron was lower than that of cabbage and spinach in the control group. In addition, five kinds of determination samples contained citric acid, succinic acid, fumaric acid and other organic acids, among which Pteridium aquilinum, Pteridium revolutum and Tectaria decurrens also contained a small amount of tartaric acid, which provided scientific basis and basic data for the development and utilization of wild ferns in the future.

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Extraction separation and determination of kaempferol in Camellia euphlebia Merr.ex Sealy

CHEN Qiu-hong, LIU Peng, WEI Ying-liang, LIU Bu-ming, HUANG Yan, MO Jian-guang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 156-158. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.032

Abstract （377）

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Solvent extraction method was used to extract and separate the ethyl acetate part of dried leaves of Camellia euphlebia Merr.ex Sealy, and determine its content. The results showed that the compound kaempferol was first isolated from the ethyl acetate part of Camellia euphlebia Merr.ex Sealy; the content determination method of kaempferol in Camellia euphlebia Merr.ex Sealy was established by HPLC. The kaempferol ranged from 0.10 to 0.40 μg/mL. The internal linear relationship was good, the average recovery rate was 99.84% （RSD=1.59%）, and the measured content of kaempferol was 0.318 2~0.364 0 mg/g.

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Analysis of volatile components in folium isatidis and radix isatidis by HS-SPME-GC-MS

TIAN Yu, JIANG Han-mei, HAN Man, CAI Meng-ying

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 159-161. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.033

Abstract （379）

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The volatile components of folium isatidis and radix isatidis were analyzed and compared by headspace solid phase microextraction （HS-SPME） combined with GC-MS. The relative percentage of each component was calculated by area normalization method. Results showed that 38 peaks were isolated from folium isatidis and 31 components were identified, accounting for 81.58% of the volatile components. 35 peaks were isolated from radix isatidis and 28 components were identified, accounting for 80.00% of the volatile components. There are 16 kinds of volatile components in the two, which account for 48.16% and 48.40% of the total volatile components of folium isatidis and radix isatidis, respectively. There were some differences in the volatile components and content of folium isatidis and radix isatidis, and they have some of the same pharmacological effects.

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Design on automatic poultry house monitoring system based on service discovery

YU Qun, ZHANG Feng-hang, WANG Xiu-li, ZHOU Zhu-nan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 162-165. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.034

Abstract （363）

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In order to improve the monitoring problem caused by the poor network environment of the poultry house, a scalable, highly available, automatically adjusted poultry house monitoring and adjustment system was designed and developed based on the analysis of the poultry house monitoring data. The standard interface design of historical monitoring data solved the problem that the traditional wireless real-time monitoring service in the production environment was poorly usable, the monitoring station was difficult to expand, and the regulation was too dependent on labor. The test results confirmed that the system design was feasible and realizes the purpose of automation,high quality and intensive farming.

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Design of a chestnut autonomic harvesting and husking machine

LIU De-yao, YOU Ying, ZHANG Yu-ting, LIU Shu-guang, TANG Li-juan, YIN Bao-quan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 166-169. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.035

Abstract （416）

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In order to solve the underlying problems of intensive manual labour, low working efficiency and backward traditional machinery in harvesting and husking process of chestnut production, a chestnut autonomic harvesting and husking machine with Raspberry Pi 3 Model B+ as control core, Arduino as development platform and battery and electric motor as power was designed. The designed chestnut autonomic harvesting and husking machine can realize autonomic track walking in the chestnut orchard by the control system; the harvest of chestnut can be realized by the rotation of bristle brush with high harvesting accuracy; chestnut husk is removed by the extrusion of PTFE hoses driven by the rotation of twin rollers. The whole machine has simple structure and strong practicability, which can provide suggestions for further improving the production efficiency of chestnut.

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Research on the biaxial solar energy automatic tracking device based on orthogonalmetering

ZHAO Hui, YANG Zheng-rui, CHEN Zhi-yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 170-172. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.036

Abstract （363）

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Based on the research of the biaxial tracking system, the driving mechanism of the biaxial tracking system wasdesigned, and the dynamic characteristics of the driving mechanism of the altitude-angleand azimuth-angle wereanalyzed. The metering module composed of four photoelectric sensors weredesigned, which was the orthogonalmetering module. The control system wascomposed of the single chip microcomputer.The results showed that the orthogonalmetering module and the motors to realize the real-time tracking of the sunlight.

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Study on the relationship between trade opening, technological progress and agricultural economic growth

MA Qian-li

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 173-178. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.037

Abstract （384）

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Based on the sample data from 2000 to 2018 and MS-VAR model, this study analyzed the relationship between trade opening, technological progress and agricultural economic growth. The results showed that, there are two obvious regional systems in the relationship between trade opening, technological progress and agricultural economic growth. At present, the expansion of trade opening can play a great role in promoting agricultural economic growth. The government should make effective use of the development stage of mutual promotion of trade opening, technological progress and agricultural economic growth, so as to ensure the rapid and sustained growth of agricultural economy. Through the transformation probability matrix of regional system, we can find that the relationship between trade opening, technological progress and agricultural economic growth is relatively stable. Therefore, the government should focus on formulating long-term policies to promote agricultural economic growth. From the analysis of impulse response, it can be seen that in the process of transformation from regional system 1 to regional system 2, the impact response of trade opening and technological progress on agricultural economic growth increases gradually. In the current stage, we should focus on the promotion of trade opening and technological progress to promote agricultural economic growth.

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Ecological feasibility analysis of the land development projects

HUANG Hong-sheng, ZHANG Hong-tao, ZHONG Hai-yan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 179-183. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.038

Abstract （362）

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As the main way to supplement cultivated land, land development has important practical significance for realizing cultivated land requisition-compensation balance. In order to prevent ecological degradation during the implementation of the land development projects, it is necessary to carry out the ecological feasibility analysis. In this study, the comprehensive evaluation index system of ecological benefits of the land development projects was established, and the evaluation index weight was determined by the analytic hierarchy process （AHP） method, the comprehensive index of ecological benefits before and after the land development projects was calculated according to the comprehensive evaluation model so as to analyze the ecological feasibility. Taking the land development projects in Fengxin county of Jiangxi province as an example, after the quantification and standardization of 11 evaluation indexes, the comprehensive index of ecological benefits before the implementation of the land development projects is 100, and after is 73.91. The results showed that, the ecological degradation during the implementation process of the land development projects, and put forward corresponding governance measures to provide basis for the implementation of the land development projects decision-making.

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Research on big data high-speed railway tourism effect based on coupling model：Take Baoji city as an example

HU Ke-ke, WAN Hong-lian, MAO Nan, CUI Shu-han, CHEN Xiao-xin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 184-188. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.039

Abstract （456）

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Taking Baoji city in Shaanxi province as the research area, the coupling model of data processing was established by means of range standardization and entropy method, to explore the coupling coordination relationship between the development level of big data and high-speed railway tourism, and analyze the impact of big data and high-speed railway on tourist destinations development. The results showed that in the context of the development of big data from 2013 to 2017, the high-speed railway tourism in Baoji city was on the rise. However, due to the influence of per capita disposable income, high-speed railway lines and big data platform, the comprehensive development present nonlinear changes, and from 2015 to 2016 the coupling degree of the two was on the edge of imbalance, and the coordination degree was moderate.

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Study on the relationship between the land use type changes and urbanization level：Take Nanjing city as an example

WANG Lan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 189-193. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.040

Abstract （318）

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There was a close relationship between the land use type changes and urbanization level. Relevant data of the land use type changes and urbanization level in Nanjing city from 2005 to 2015 were selected, and correlation analysis and regression analysis were used to discuss the relationship between the land use type changes and urbanization level. The results showed that,as the level of urbanization increases, the types of land use also changes. Among them, the area of agricultural land and unused land continues to decrease as the level of urbanization increases, while the area of construction land continues to increase as the level of urbanization increases. The level of urbanization affects the land use type changes. In the process of steady and rapid urbanization, it is necessary to rationally develop and use land, and maintain correct land use planning, in order to provide a theoretical basis for urban rational development and land use.

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Research on the problems of family support for the empty nest elderly in rural areas from the perspective of intergenerational relationship：Take H province as an example

CHEN Ji-hua, LU Xin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 194-197. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.041

Abstract （372）

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Based on the perspective of intergenerational relationship and the 2016 data of Chinese family tracking survey （CFPS）, this study analyzes the current situation and existing problems of the family support for the empty nest elderly in rural areas of H province, summarizes the reasons affecting the intergenerational support for the elderly in rural areas, analyzes and proposes suggestions for improvement.

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Study on organizational ecosystem of characteristic towns from the perspective of organizational ecology：Take Longtoushan prickly ash characteristic town as an example

ZHANG Lu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 198-202. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.042

Abstract （337）

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As the new comprehensive development space platform integrating characteristic industry,social life and cultural construction,characteristic towns realize the maximum industrial development,upgrading and transformation in the well-defined region with new ideas,new mechanisms and new carriers. Therefore, based on the theoretical perspective of organizational ecology to explore the internal composition of the organizational ecosystem of characteristic towns, take Longtoushan prickly ash characteristic town in Hunnan province as the practice case, analysis its internal organizational ecosystem from the inside to the outside of the hierarchical organization, cluster association, combination of reality, and from industry, function and system of the three dimensions to explore the development path and strategy.

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Research on the development of rural research tourism products in Huzhou city based on RMP analysis

ZHU Zhi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 203-207. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.043

Abstract （561）

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The rural tourism resources in Huzhou city were sorted out and classified. Based on the RMP analysis mode of tourism product development theory, the development status of rural research tourism products in Huzhou city was analyzed. According to the characteristics of rural tourism resources in Huzhou city, the development ideas of rural research tourism products were put forward.

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The practice, content and experience of 70 years poverty alleviation and development in China

SUN Zhong-gen, CAO Zhi-dong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 208-211. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.044

Abstract （364）

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Since the founding of the People’s Republic of China 70 years ago, the government has carried out poverty alleviation and development practice in various fields, including industrial poverty alleviation, special poverty alleviation, urban and rural integration, and social security coverage. By sorting out the practice and content of China’s poverty alleviation and development, it is concluded that we should adhere to the concept of “people-oriented”, play a leading role in the system, and constantly summarize and improve the mode. We should adhere to the concept of poverty alleviation through development, consolidate the achievements of poverty alleviation and development, and pay attention to the promotion of poverty alleviation and development experience.

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Research on the construction mode of beautiful countryside in Shangluo city under the background of rural revitalization strategy

LIU Long-long, GAO Zhan-hai

HUBEI AGRICULTURAL SCIENCES 2020, 59 (14): 212-216. DOI: 10.14088/j.cnki.issn0439-8114.2020.14.045

Abstract （395）

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During the 13th Five Year Plan period, Shangluo city of Shaanxi province, based on the advantages of ecology, resources, location, culture, and the brand advantage of “Shangluo is the most beautiful countryside in Qinling”, this study proposed to build the beautiful countryside in Qinling and vigorously develop rural tourism. Based on the analysis of the current situation and model types of beautiful countryside construction in Shangluo city, point out the problems existing in the construction of beautiful countryside in Shangluo city, and put forward three modes of agricultural production, cultural inheritance and leisure tourism, and the key points of construction and development of the three modes.

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Research progress on rare microbial community

GUAN Jian-fei, SHEN Zhi-chao, CAO Yang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 5-7. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.001

Abstract （401）

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Microbes have a profound impact on people′s lives and they also affect the global biogeochemical cycle. A rare microbial community has an important ecological role, in evaluating microbial diversity, a repository with genetic and functional diversity, and serves severe important functions, such as driving the geochemical cycle, responding to environmental changes, degrading pollutants, and stabilizing the community structure. The concept, types, research methods, existing mechanism and function of rare microbial community were introduced, which could provide reference for further study of microbial biogeography and exploration of microbial genome.

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Development and demand analysis of liquid fertilizer machines at home and abroad

ZHANG Lu-yun, HE Yi-chuan, YANG Huai-jun, HE Xing-cun, MENG Xiang-jin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 12-15. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.002

Abstract （573）

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Liquid fertilizer has many advantages, such as easy to be absorbed by crop roots, less pollution to the environment, flexible formulation and convenient application. It is a new type of fertilizer developed rapidly in China in recent years. The development status of liquid fertilization machinery at home and abroad was analyzed. The structure and working principle of several main liquid fertilizer applicators were summarized. The application status of liquid fertilizer applicators in China was discussed. The corresponding countermeasures and suggestions were put forward to promote the rapid development of resource-saving and environment-friendly agricultural industry.

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Chemical constituents and development and utilization of mulberry leaves

ZHANG Qian, ZHANG Li-hua

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 16-19. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.003

Abstract （765）

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The research progress on the chemical constituents and development and utilization of mulberry leaves in recent years was summarized. The active components such as flavonoids and flavonoid glycosides, polyphenols, polysaccharides, alkaloids, sterols, volatile oil, amino acids and vitamins in mulberry leaves were mainly introduced. The pharmacological functions of mulberry leaves, such as lowering blood lipid, lowering blood sugar, anti-aging, anti-virus and anti-tumor,wereintroduced. The development and utilization of mulberry leaf products were summarized.

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Effects of sowing date and density on yield and quality of different maturity cotton varieties at cotton planting region of Poyang lake

ZHANG Li-juan, XIA Shao-nan, LI Yong-qi, XIE Ye-tao, YANG Tai-yang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 20-24. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.004

Abstract （435）

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To explore the possibility of replacing early-maturing varieties with medium early-maturing varieties at cotton planting region of Poyang lake,two representative cotton varieties of medium early-maturing variety Ganmianza No.1（C₁） and early maturing variety Zhongmiansuo425（C₂） were planted at three sowing date （A₁.April 28, A₂.May 10, A₃.May 26） and two densities （B₁.67 500 plants/hm², B₂.90 000 plants/hm²） using the crack zone design in 2018, and the effects of sowing date and density on yield, yield characteristics and fiber quality of cotton were studied. The results showed that the seed cotton yield of all treatments were from 2 513.5 to 3 072.0 kg/hm², and the yields of A₁B₁C₁ and A₁B₂C₁ were relatively high. The yield of A₂B₂C₂ was the lowest, and the difference between the treatments of A₃ was not significant. Sowing date had a significant effect on fiber quality. The quality of cotton fiber treated in A₃ was better than that of A₁ and A₂. In summary, the medium early-maturing varieties should be suitable for late sowing （before the end of May） at cotton planting region of Poyang lake, and as long as the density is appropriate and reasonable chemical regulation, they can grow and develop through their own automatic regulation mechanism to get high yield without affecting fiber quality, and can be used instead of early maturing varieties.

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Application of winterness and springness identification of wheat on regional trial

MENG Li-mei, YANG Zi-guang, ZHANG Ke, SUN Jun-wei, JI Tian-hui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 25-27. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.005

Abstract （452）

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The wheat （Triticum aestivum L.） varieties （lines） from regional trial in southern Huang-Huai region and Huang-Huai dryland region from 2010 to 2019 were selected as materials. Field identification method of spring sowing was used to identify the winterness and springness of wheat according to the calculation of the earing stage and heading rate of spring seedling. The results showed that a total of 32 springness varieties （lines） were identified from winter wheat regional trial in southern Huang-Huai region and Huang-Huai dryland group,accounted for 4.78% of the tested varieties （lines）; A total of 18 winterness varieties （lines） were identified from spring wheat regional trial in southern Huang-Huai region, accounted for 21.43% of the tested varieties （lines）. From the appraisal situation, the method that setting of sowing date in recent 10 years is based on the local average temperature in spring, the first sowing date is the next day after the average temperature reaches 3 ℃, the second sowing date is the next day after the average temperature reaches 7 ℃, and the third sowing date is the next day after the average temperature reaches 10 ℃, was not restricted by time interval and was accurate and reliable through the 3 sowing dates confirmed each other. Among the tested varieties （lines）, the heading rate of varieties （lines） identified as winter type at the second sowing date in spring was less than 30%, and the plants was immature at the mature stage; The heading rate of varieties （lines） identified as spring type at the second sowing date in spring was more than 30%, the plants was basically mature at the maturity stage, and the seedling ear stage was more than 45 days. It indicated that the heading rate and seedling ear stage of spring sowing could be used as effective detection indicators for winterness and springness identification of wheat.

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Analysis of two heavy precipitation under the control of subtropical high in Hunan

JIANG Shuai, ZHOU Li, YIN Yi-wen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 28-36. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.006

Abstract （451）

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Based on the station of national, regional station data and NCEP reanalysis data, the two process of heavy precipitation in Hunan on July 19 （0719） and September 4 （0904） under the control of subtropical high were comparatively analyzed. The results show： ①The two process subtropical highs were relatively strong, but their positions were quite different. The main body of “0719” was easterly, while the main body of “0904” was westerly. 925 hPa shear had a good indicating significance for heavy precipitation areas; ② The middle and lower layers of the precipitation area were convective unstable stratification, and 700~500 hPa was neutral stratification, which was conducive to the occurrence and development of precipitation. ③ It is found that the water vapor sources in the two processes were different by the air parcel tracking method. Water vapor mainly came from the western Pacific region in “0719” process, and came from the Indian Ocean region in the process of “0904”. ④ In terms of quantities, the energy of water vapor was vertically divided into unstable stratification of upper dry cold and lower wet warm wet in the “0719” process. In addition, the process vertical rose motion, convergence and divergence, and relative vorticity configuration were shallow, mainly concentrated in the middle and low layers. In the “0904”process, the wet layer was thick in the vertical direction. Influenced by cold air, there was an obvious zone, on the one hand, convergence rose with the warm current rendezvous, lifting up on the other hand, to reinforce upward movement, trigger instability energy, and process of vertical ascending motion area extended upward height higher than the “0719”, low-level convergence layer deep, relative vorticity upload more, corresponding to the greater the rainfall intensity.

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Diagnostic analysis of a rare persistent rainstorm in late autumn in Hunan province

LIU Hong-wu, TANG Lin, LIAO CHUN-hua, WANG Qing-xia, DENG Zhao-ping

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 37-43. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.007

Abstract （431）

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Based on the conventional meteorological observation data and 1 °× 1 ° NCEP reanalysis data, a comprehensive analysis of a rare continuous heavy rain weather process occurred in the central and northern Hunan province from October 27 to November 1 was carried out, 2014. The results showed that the intensity and location of the subtropical high, the activity of the low trough in the Bay of Bengal, and the east-west high-low situation were the large-scale circulation backgrounds that caused the heavy rain process; The high-level divergence, low-level convergence and strong ascending motion provided powerful dynamic lifting conditions for the rainstorm; The establishment and maintenance of the low-level southwest jet provided abundant water vapor conditions for the rainstorm. During the whole heavy rainfall process, the cold air activity was weak, and the rainstorm mainly occured in the water vapor convergence area on the left side of the southwest jet. The heavy rain in late autumn mainly occured in two stages. The first stage was the stable atmospheric stratification under the influence of the cold air from the east road, with a long duration; The second stage was the invasion of the cold air from the west road, with a short duration under the convective instability.

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Analysis of vegetation cover change and climate driving factors on the Qinghai-Tibet plateau

XUE Yu-xuan, LU Hong-wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 44-48. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.008

Abstract （495）

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MODIS product （NDVI and land surface temperature） and meteorological data （precipitation and temperature） from 317 stations of the Qinghai-Tibet plateau from 2001 to 2015 were used to study the correlation coefficient between NDVI and temperature, precipitation and land surface temperature, and the combined driving mechanism of three climatic factors was discussed. The results indicated that the vegetation decreased from east to west and from south to north, and the vegetation growth trend was positive on the Qinghai-Tibet plateau. The vegetation was positively correlated with precipitation, temperature and ground temperature. The vegetation had the highest correlation coefficient with temperature on the Qinghai-Tibet plateau. The primary driving factor for vegetation growth is heat. Precipitation and heat play a complementary role in the growth of vegetation on the Qinghai-Tibet plateau.

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Effects of phosphate-solubilizing bacteria inoculated with different phosphorus levels on lettuce growth and soil phosphorus forms

DU Lei, CHEN Gang, WANG Su-ping, HONG Juan, HUANG Xiang, ZHANG gui-you, LIAN Zhi-cheng, JIANG Li, YE Li-xia, ZHANG Li-hong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 49-53. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.009

Abstract （429）

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A potting experiment was conducted to evaluate the effects of the phosphate-solubilizing bacteria on lettuce growth and soil phosphorus forms at different growth stages of lettuce （Lactuca sativa var. ramosa Hort.） under four application levels of calcium-superphosphate. The results showed, under the condition of soil phosphorus deficiency, that the application of the phosphate-solubilizing bacteria could improve the leaf fresh weight, root fresh weight,leaf dry weight and root dry weight of lettuce, increased the net photosynthetic rate and transpiration rate, enhanced the phosphorus absorption capacity in lettuce plants, improved the nutritional quality and yield of lettuce, reduced the accumulation of whole phosphorus content in soil, and promoted the release of effective phosphorus and the accumulation of effective phosphorus in soil reservoir. In the case of the soil phosphorus abundance, the application of the phosphate-solubilizing bacteria was weaker influence on the growth of lettuce and soil phosphorus. When the amount of phosphate fertilizer in the soil was 0.375 g/kg, the phosphate-solubilizing bacteria promoting growth effect of lettuce and the soil phosphorus to release were the best. The application of the phosphate-solubilizing bacteria not only decrease the loss of phosphorus in agricultural land, mitigate the risk of phosphorus non-point source pollution, but also guarante the quality and quantity for farmers to maximize vegetable yield.

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Effects of new compound fertilizer Heijindao on yield and lodging resistance of direct seeding rice

LIU Hang-jiang, TANG Le-dan, CHEN Guo-xing, SHI Shi-jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 54-59. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.010

Abstract （511）

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In order to explore the application effects of the new compound fertilizer Heijingdao in production, taking hybrid rice variety Longliangyou 1988 and conventional rice variety Huanghuazhan as experimental materials, two fertilizer treatments were set and used common compound fertilizer as control to analyze and compare the effects of different fertilization treatments on rice yield formation and lodging resistance by field plot experiment. The results showed that, compared with control, the effects of Heijingdao compound fertilizer could effectively increase rice yield, and the main reason was that the the number of effective panicles and 1 000-grain weight were significant increase. It can increase the number of the highest stems and the number of effective stems of rice, but the final earing rate was not much different compared with that of common compound fertilizer. The increase of stem number made the leaf area index of each growth period significantly higher than that of the control. Dry matter accumulation and crop growth of rice applied Heijingdao compound fertilizer also showed significant advantages, ensuring the formation of photosynthetic products. This compound fertilizer can significantly improve the lodging resistance-related characters in the mature stage, the ratio of internodes of rice applied Heijingdao compound fertilizer was optimized to reduce the plant height and barycenter height of hybrid and conventional rice, and increased the basal internode thickness and wall thichness of the stem, so that the flexural strength of basal internode increased and lodging index decreased.

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Effects of cover plant in winter on soil CH₄ and N₂O emission under the ratoon rice system

LYU Ze-fang, GAO Zhen-zhen, LIU Zhang-yong, JIN Tao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 60-65. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.011

Abstract （351）

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In order to study the effects of cover plant in winter on the CH₄ and N₂O emissions from ratoon rice system, the CH₄ and N₂O flux was observed by the static closed chamber-gas chromatography method under the treatment of ratoon rice-fallow （CK）, ratoon rice-rapeseed （FR）, and ratoon rice-milk vetch （MR）, respectively. Results showed that the CH₄ and N₂O flux of all treatments was low in the winter cover plant growing season, and was high in the rice growing season. The cumulative CH₄ emissions in the main rice growing season occupied 85.89% to 97.48% of annual cumulative CH₄ emissions. During the main rice growing season, the cumulative CH₄ emissions in the treatment of MR was significantly lower than that in the treatment of CK and FR. Over the ratoon season, there was no significant difference in the cumulative CH₄ emissions among each treatment, and which occupied 2.02% to 14.11% of annual cumulative CH₄ emissions. The cumulative N₂O emissions in the main rice growing season was 1.74 to 2.43 times of ratoon season. The cumulative N₂O emissions of MR was significantly lower than that of CK in the main rice season, and there was no significant difference in the cumulative N₂O emissions among each treatment in the ratoon season. The total annual global warming potential （GWP） of CH₄ and N₂O for each treatment was in decreased order of FR, CK, MR. And the GWP of CH₄ and N₂O in the rice growing season was significantly higher than that in the winter cover plant growing season. Meanwhile, the contribution of CH₄ emission to GWP in rice growing season was much higher than that of N₂O. Therefore, the main rice season is the key season for reducing the greenhouse gas emissions. And plant milk vetch in the winter season could reduce the CH₄ and N₂O emissions under the ratoon rice system compare plant rapeseed or fallow.

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The evolution characteristics and the construction of climate hazard risk index of soybean autumn frost in Inner Mongolia

WANG Hui-zhen, TANG Hong-yan, LI Dan, WU Xiang-dong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 66-70. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.012

Abstract （348）

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Based on the lowest daily temperature and the earliest frost occurrence date of 119 meteorological stations in Inner Mongolia Autonomous Region from 1981 to 2010, the maturity date of the 80% guarantee rate of different climate ecoregions was used as the boundary date to assess whether the soybean [Glycine max （Linn.） Merr.] frost disaster occurred. The climate hazard risk index system of frost disasters was constructed to analyze the evolution characteristics of Inner Mongolia soybean autumn frost and spatial distribution characteristics. The results showed that in the 30a time scale, different grade autumn frost of soybean occured in Inner Mongolia were slightly smaller trend, but the early frost days of different grades showed an early trend. The regional characteristics of frost occurrence were obvious, and the range of light frost occured in relatively large areas, and the interannual variation of light frost was obvious, and the date of occurrence was also significantly in advanced. The distribution law was that the frequency of autumn frost in different grades was generally high in the northeast, was low in west and southeast. The coefficient of variation and the spatial distribution of intensity and frequency were relatively consistent, and the regions with large variability were mainly distributed in the Daxinganling mountains and Yinshan mountains. Through the construction of climate hazard risk index model and classification of risk climate index, there was a large spatial difference for the risk of autumn frost in Inner Mongolia, the area with high risk of frost was concentrated in the northeast region. And among them, the south of Daxinganling was agricultural area that was one of the major soybean producing areas of Inner Mongolia. There is an advantageous condition for ensuring a high harvest of soybeans that mastering the change law of frost, rationally arranging crop varieties and familiarity, doing a good job in disaster prevention and mitigation and meteorological services for agriculture in the region.

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Physiological response of Vigna radiata L. under cadmium stress

ZHANG Xiao-juan, ZHUANG Feng-hong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 71-74. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.013

Abstract （386）

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Hydroponic culture was conducted to study the effect of different concentrations of cadmium stress on the growth and physiological and biochemical characteristicsof seedings of Vigna radiata L.. The results showed that the plant height and fresh weight of seedings of Vigna radiata L. were inhibited with the increase of Cd²⁺ concentration; The chlorophyll content of the leaves of seedings decreased, but malondialdehyde （MDA） content, superoxide dismutase （SOD）activities, peroxidase （POD） activities and catalase （CAT） activities gradually increased with the increase of Cd concentration,while the soluble sugar content increased at the beginning then decreased. The results indicated that the Cd²⁺ stress would destroy the biofilm function of Vigna radiata L., caused the oxidative intimidation and affected their growth and development.

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Evaluation and variation characteristics of climatic comfortability of human settlement environment in Tangshan city

SHI You-yu, CHAI Rui, WANG Ai-jun, CAO Ya-jie, FEI Xiao-chen, SUN Yun-suo

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 75-79. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.014

Abstract （432）

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Using the meteorological data of Tangshan National Basic Meteorological Station from 1957 to 2018, the climatic comfortability of Tangshan city was evaluated based on the evaluation method of climatic suitability evaluating human settlement, and its changing characteristics were analyzed. The Mann-Kendall mutation test was carried out to analyze the annual and seasonal change of comfort index. The results showed that the climate comfort of Tangshan city was cold from January to April and from November to December, and it was comfortable from May to September and cold in October. The climate comfort index of the human settlement environment showed a linear upward trend. The monthly climate comfort index presented an inverted “V” type change, with the highest in July and the lowest in January. There was no mutation in the annual climate comfort index. The spring index had a mutation in the early 1990s. There was no significant mutation for the summer index. The autumn index had a mutation in the early 1970s, and the winter index had a mutation in the mid-early 1970s.

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Effects of systemic insecticides on biological activity and instantaneous rate of population increase of Aphis gossypii Glover

YANG Fan, ZHAO Qian-qian, WANG Pan, ZHOU Li-lin, SI Sheng-yun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 80-85. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.015

Abstract （436）

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In order to explore the new way of high precision application on cucumber （Cucumis sativus Linn.） plant, and screen efficient systemic insecticides against Aphis gossypii Glover, the corrected fatality rate and the instantaneous rate of population increase （r_i） of nine insecticides against A. gossypii Glover were evaluated by stem application. The results showed that the corrected fatality rate of acetamiprid 20% soluble concentrate and flonicamid 10% grain agent were significantly higher than the other insecticides. The corrected fatality rate of these two insecticides were nearly 100.0% on the tenth day and fifteenth day, and were still above 95% until twenty days after application. Flonicamid 10% grain agent had poor efficiency, but better persistance. The maximum mortalities of imidacloprid 70% grain agent and nitenpyram 10% aqueous solution were 99.3% and 95.0% on the tenth day, respectively, and were all above 90% until twenty days after application. The maximum corrected fatality rate of thiamethoxam 25% grain agent, cyantraniliprole 10% suspension concentrate, pymetrozine 25% wettable powder, dinetofuran 20% grain agent and spirotetramat 22.4% suspension concentrate were all below 90%. Under the treatment of medium and high concentration, acetamiprid 20% soluble concentrate and imidacloprid 70% grain agent had significant stage inhibiting effects on the population increase in the middle of the trail, whereas flonicamid 10% grain agent showed constant inhibition in the later stage. Acetamiprid, imidacloprid and flonicamid had higher systemic activity to A. gossypii Glover in cucumber plant.

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Effect of drought stress on light response characteristics of photosynthesis of Cyclobalanopsis gilva seedlings

WU Li-jun, LI Zhi-hui, YANG Mo-hua, LI Chen-xi, LI Jia-luo, LI An

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 86-89. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.016

Abstract （422）

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Aiming to illustrate the photosynthetic response and adaptation mechanism of Cyclobalanopsis gilva to drought stress, simulating four soil water gradients in pot culture, the one-year-old seedlings of C. gilva from three sources were used to measure the photosynthetic light responses under drought stress. The results showed that, under different drought stress treatments, the light response curves of C. gilva seedlings from three provenances were basically the same. The non-rectangular hyperbola model fit well with the light response curves of C. gilva with the determination coefficient of 0.95. With the increase of drought stress, the maximum net photosynthetic rate（P_n_max）, the apparent quantum yeild（AQE） and dark respiration rate（Rd） of the three provenances showed a decreasing trend. The seedlings of Dongkou provenance of Hunan province showed some adaptability and tolerance under mild and moderate drought stress, which showed that the Dongkou provenance of Hunan province had a stronger adaptability to drought stress among three provenances.

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Effects of four substrates on the leaf cutting propagation of Primulina macrorhiza

YAN Hai-xia, GUAN Shi-kai, SONG Qian, ZHOU Jin-ye, LA Yan-fei, LUO Shu-ming

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 90-93. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.017

Abstract （354）

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In order to find out the effects of different matrix components on cutting propagation of Primulina macrorhiza, taking the whole leaves of P. macrorhiza as experimental materials, orthogonal experimental design was conducted to study the effects of four substrates including peat, perlite, vermiculite and rice husk charcoal on leaf cutting survival rate, number of cotyledons and total number of new leaves, and the optimum combination was selected. The results showed that among the nine treatments, the ratio of peat, perlite, vermiculite, rice husk carbon, for 4∶2∶2∶1（volume ratio） was the best cutting medium. Peat had significant effects on survival rate, number of cotyledons and total number of new leaves（P<0.05）; Perlite had no significant effects on survival rate, number of cotyledons and total number of new leaves（P>0.05）; Vermiculite had significant effects on total number of new leaves only（P<0.05）; Rice husk charcoal had significant effects on cotyledons only（P<0.05）. Orthogonal analysis showed that the best ratio of peat, perlite, vermiculite and rice husk charcoal was 2∶1∶1∶1（volume ratio） when the four components were used as cutting substrate.

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Effects of different organic fertilizers on growth and physiological indicators of Mirabilis jalapa

BAI Xiao-xia, CAO Lei, AI Hai-jian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 94-96. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.018

Abstract （410）

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In order to understand the effects of different organic fertilizers on the growth and development of Mirabilis Jalapa L., different organic fertilizers were used to conduct experimental research on annual M. Jalapa. The rules of soil nutrient content and growth indexes under different organic fertilizers treatment were analyzed. The results showed that the soil nutrient content was obviously increased by applying different organic fertilizers, and the overall variation trend of soil nutrient content was the same under different treatments. The application of organic fertilizer had an obvious promoting effect on plant growth and root activity. The application of organic fertilizer could increase the photosynthetic rate and decrease the transpiration rate of M. jalapa, and enhance the resistance of plants.

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Research on the construction of eco-tourism destinations for mountain and water of Ankang city

ZHAO Lin-long

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 97-103. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.019

Abstract （363）

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Ankang city of Shaanxi province plays the role of connecting link, connecting east-west, radiating surrounding in the north-south tourism corridor and Han River ecological economic belt. In view of the three key problems in the development of Ankang tourism strategy including the utilization of the advantages of the geographical location of Ankang “center”, the construction of the Ankang-Zhangjiajie high-speed railway project and the coordinated development of the Han River eco-economic belt, the constructive suggestions are put forward in order to play a positive role in the development of Ankang tourism.

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Selection of suitable forage grass species in the rocky desertification area of Guangxi

DENG Su-yuan, YI Xian-feng, PANG Tian-de, YAO Na, CAO Shu-wei, SHI Jing, HUANG Yi-long, LIANG Yong-liang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 104-107. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.020

Abstract （427）

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In order to screen out the herbage varieties suitable for planting in the harsh ecological environment of Guangxi rocky desertification area and improve the ecological environment, 14 high-yield and high-quality herbage varieties were introduced for planting on the hillside in Guangxi rocky desertification area to observe the growth adaptation and measure the plant height, yield and soil nutrient changes. The results showed that Pennisetum purpureum Schumab cv. Purple, Aeschynomene americana L., Tephrosia candida DC., Paspalum wettsteinii Hackel and Stylosanthes guianensis （Aubl.） Sw had a better performance, and their coverage were up to 80%~100%, which were competitive with weeds, the annual output of fresh grass of P. purpureum Schumab cv. Purple was 15.63 t / 667 m². The output of fresh grass of other varieties of A. americana L., P. wettsteinii Hackel and S. guianensis （Aubl.） Sw was 2.01, 2.51, 1.96 t /667 m², which could be cut 3 to 4 times per year. In particular, S. guianensis （Aubl.） Sw could significantly increase the content of soil organic matter by 7.12% compared with the control group. Meanwhile, the content of hydrolyzed nitrogen in the soil was also increased by 13.68% compared with the control group. Tephrosia candida DC. seeds germinated less in the first year because of seeds hard, but the coverage also reached 80% in the second year. Therefore, P. purpureum Schumab cv. Purple, A. americana L., T. candida DC., P. wettsteinii Hackel and S. guianensis （Aubl.） Sw were suitable for planting in the rocky desertification area of Guangxi, and the control effect of rocky desertification was better. Meanwile, forage grass can be used for cattle and sheep breeding, developing rocky desertification mountain animal husbandry.

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Comparison and preliminary application of five extraction methods of egg yolk antibody

XIANG Dong-mei, YI Si-yu, TANG Qing-hai, WU Si-wen, WANG Shu-shu, LI Yan, LI Lu, HAN Tao-tao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 108-113. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.021

Abstract （508）

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In order to obtain a relatively good extraction process of egg yolk antibody, five methods including octanoic acid method, water dilution method, chloroform method, ammonium sulfate precipitation method and polyethylene glycol precipitation method were improved in this experiment. Expression of recombinant porcine pathogenic Escherichia coli toxin STxB（rSTxB） was induced by IPGT, and then the immunogen was prepared by emulsifying the rSTxB with the adjuvant 71VG, and the hens were immunized, IgY was extracted by modified polyethylene glycol method. The purity of the IgY antibody was detected by SDS-PAGE electrophoresis, and the immunoactivity and titers of yolk antibody and serum antibody titer were detected by Western blot. The results showed that the extraction efficiencies of octanoic acid method, water dilution method, chloroform method, ammonium sulfate precipitation method and polyethylene glycol precipitation method were 96.00,81.99,155.50,65.34,46.00 mg/mL,respectively, and the extraction purity of polyethylene glycol precipitation was the highest. The molecular weight of rSTxB protein was about 25 kDa, the antibody titer in serum was up to 64 000 times, and the highest dilution factor of yolk antibody was 16 000 times. For the comprehensive evaluation, the extraction method of polyethylene glycol precipitation was relatively superior, and the extracted yolk antibody showed good immunological activity.

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Optimization of extraction technology of polysaccharide in Polygonatum sibiricum by orthogonal method

WANG Yi, MU Qi-lin, TIAN Xiao-hai, WU Yun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 114-117. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.022

Abstract （467）

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The stems and leaves of medicinal economic plant Polygonatum sibiricum in Enshi city were used as test materials, and water was used as the extraction solvent to test the stems and leaves of Polygonatum sibiricum respectively, the optimized extraction process was obtained. The study found that when the ratio of material to liquid was 1∶20 （g∶mL）, the extraction time was 2 h and the temperature was 80 ℃, the yield of polysaccharide from Polygonatum sibiricum stems was 19.87%, the yield of polysaccharides from Polygonatum sibiricum leaf was 17.13%. The results show that the polysaccharide content in the stems of Polygonatum sibiricum are higher than that in leaves, but in general, the polysaccharide content in stems and leaves of Polygonatum sibiricum are still relatively high, and industrial production can be considered for further utilization.

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Analysis of some quality characters of waxy wheat and non waxy common wheat

CUI Wen-li, WANG Jun, WANG Hui, YAO Da-nian, SONG He, ZHENG Wen-yin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 118-121. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.023

Abstract （590）

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Waxy wheat and common wheat varieties （lines） were used as experimental materials to study the differences of some quality characters. The results showed that the average values of grain hardness, bulk density, water absorption, stability time, formation time and flour yield of waxy wheat were lower than those of common wheat, but the average values of grain protein content, wet gluten content and sedimentation value of waxy wheat were higher than those of common wheat. Compared with the three waxy wheat varieties, the hardness, sedimentation value and stability time were different.

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Study on the correlation between potassium dichromate external heating method and ASI for soil organic matter determination

HUANG Xiang, DU Lei, HONG Juan, CHEN Gang, WANG Su-ping, LIAN Zhi-cheng, ZHANG Gui-you, JIANG Li, ZHANG Li-hong, YE Li-xia

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 122-125. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.024

Abstract （484）

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The correlation between ASI method and potassium dichromate external heating method for soil organic matter determination is compared. The results showed that under neutral and alkaline soil conditions, the ASI method and the potassium dichromate external heating method were significantly positive correlation, and there was a certain proportion coefficient between the test values of the two methods.

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Rapid determination of molinate residues in rice by gas chromatography

MENG Qing-shun, SUN Li

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 126-128. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.025

Abstract （464）

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A method for the determination of herbicide residues in rice was established by gas chromatography. The method was validated by flame photometric detector FPD, external standard method and automatic injection. The results showed that the detection limit of diclofenac sodium was 2 μg/kg （injection volume was 1 μL, S/N 3）, the linear range of standard curve was 10~100 μg/kg （r =0.999 74）, the recovery of standard addition was 80.0%~97.5%, RSD （n=10） was 3.28%. The method has the advantages of high recovery rate, good reproducibility and short analysis time. It is an accurate, fast and simple detection method.

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Determination of active ingredients in Dachengqi decoction of rhubarb and steamed rhubarb by HPLC

QIN Su-hong, QIN Qiao-feng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 129-135. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.026

Abstract （357）

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Simultaneous determination of 10 active ingredients in Dachengqi decoction of rhubarb and steamed rhubarb by HPLC. The column was Agilent Zorbax SB-C18（5 μm×4.6 mm×250 mm）, the mobile phase was acetonitrile-0.1% phosphoric acid aqueous solution, gradient elution, flow rate was 1 mL/min, detection wavelength was 280 nm and 254 nm, column temperature was 30 ℃. The results showed that the 10 standard curves of 10 active ingredients in Dachengqi decoction of rhubarb and steamed rhubarb showed a good linear relationship in the detection range; the methodological investigation reached the test requirements. The content of 10 active ingredients in Dachengqi decoction of rhubarb and steamed rhubarb varied to varying degrees. The content of 10 active ingredients in rhubarb and steamed rhubarb has different degrees of change. These changes correspond to the effect of Dachengqi decoction in combination with raw rhubarb and steamed rhubarb, and the content of rhubarb anthraquinones to a certain extent, there is a certain influence and common relationship on the efficacy of Dachengqitang.

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Study on quality standard of Shenhong Quyu Sanjie granules

ZHONG Wen, LI Lu-qing, YE Min

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 136-139. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.027

Abstract （365）

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Thin layer chromatography was used to identify ginseng and safflower as control materials. The content of fumarate in the preparation was determined by high performance liquid chromatography,to establish the quality standard of Shenhong Quyu Sanjie granules. The results showed that the thin-layer atlases of the ginseng control medicine and the safflower control medicine showed clear spots, good separation effect, strong specificity, and no interference in the limit control. It has a good linear relationship with the peak area in 7.174~35.940 μg/mL（r=0.999 89）. The average sample recovery was good. The method is simple, accurate and reproducible, and can be used for the quality control method of Shenhong Quyu Sanjie granules.

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Mode reconstruction of fresh agricultural products e-commerce from the perspective of information economics

ZHAO Jing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 140-144. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.028

Abstract （456）

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In order to effectively alleviate the quality information crisis of fresh agricultural products, increase the profitability of fresh agricultural products e-commerce, and break through the bottleneck of high-quality development of fresh agricultural products e-commerce, based on the relationship between the quality and information of fresh agricultural products, using the theory of quality information transmission in information economics, the origin of the quality information of fresh agricultural products is analyzed from the information asymmetry between the supply and demand of fresh agricultural products e-commerce. This paper puts forward three problems existing in the e-commerce mode of fresh agricultural products, namely, the limited transmission of quality information of fresh agricultural products, the “black box” of quality information in the supply chain, and the imperfect screening mechanism of quality information. This paper reconstructs the e-commerce model of agricultural products from the production, circulation and consumption links, and proposes that the production links should be transformed from scattered small farmers to highly organized agricultural cooperatives. The circulation links should be transformed from mainly transmitting the characteristic information of fresh agricultural products search products and experience products to mainly transmitting the characteristic information of fresh agricultural products trust products. The consumption links should be transformed from passive consumers mode of information transfer from receiving information to active production of consumers.

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Research and application of floods forecasting model for small and medium rivers in Hunan province

TANG Jie, XU Jing-yu, LIU Hong-wu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 145-150. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.029

Abstract （436）

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According to the actual flood prevention needs of Hunan province, the small and medium river basins of Hunan province were divided into five major basins （Xiangjiang River, Zishui River, Yuanjiang River, Lishui River and Dongting Lake） using the natural watershed method. Based on the water levels of 27 hydrological stations and the precipitation products of the 97 meteorological stations and grids from 2008 to 2018, the relationship between heavy precipitation and floods were further studied. Thus a water level forecasting model based on river basin precipitation was established to predict whether there would be floods. The forecasting model was tested using the example of heavy precipitation and flood process from July 6^th to 13^th of 2019. Results showed that during this flood process, the forecasting peak value and changing trend of the water level were consistent with the actual water level. Therefore, the model has good effects on water level forecasting.

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Genetic character analysis of two wild mulberry varieties in Ankang based on chloroplast matK gene sequence

WANG Rui-xian, CHU Qu, PENG Yun-wu, MENG Gang, LIANG Jia-jun, ZHOU Xiao-fan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 151-154. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.030

Abstract （436）

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The matK gene sequences of two wild mulberry that collected form Huangshitan and Baxian town （Ankang city, Shaanxi province） have been obtained by PCR. The length of amplified sequences is 1 715 bp while the ORF is 1 518 bp. Only one base difference when compared with each other. The genetic variations of 7 mulberry have been analyzed with the matK genes. The results showed that 6 variable sites were detected, which defined 3 haplotypes in Morus alba, and the haplotype diversity was 0.714. The nucleotide diversity was 0.001 32 and the average nucleotide difference was 2.000 respectively. As for the genetic distances, Baxian was closer to M. indica and M. multicaulis, while Huangshitan was closer to M. mongolica and M. cathayana. Cluster analysis demonstrated that the seven mulberries were grouped together, and the branch was shorter than other species, implied lower genetic diversity and fewer evolutionary events proceeded.

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Study on tissure culture and rapid multiplication of Goodyera schlechtendaliana

FU Zhi-hui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 155-158. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.031

Abstract （361）

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Rapid multiplication and tissure culture were used to study the induction of buds and the proliferation and rejuvenation of cluster buds of Goodyera schlechtendaliana Rchh. f.. The results showed that with the increase of 6-BA concentration, the shoot proliferation rate increased, and a maximum of proliferation was obtained at 4.0 mg/L 6-BA, and multiplication of shoots could be achieved and controlled by combination of 6-BA 4.0 mg/L and NAA 0.2 mg/L. The rate of muhiplieation and growth could be improved in media supplemented 100.0 g/L coconut juice（CJ） and 0.5 g/L activated charcoal（AC）. Rooting of shoots were developed in 1/2 MS media with 0.5 mg/L IBA, 0.5 mg/L NAA and 0.5 g/LAC.

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Study on the environment of agricultural trade liberalization in China

SHEN Lei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 159-162. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.032

Abstract （328）

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The basic characteristics of agricultural trade liberalization was analyzed. The agricultural environmental effects of China's agricultural trade liberalization, including technical effect and regulatory effect, scale effect and terms of trade effect, structural effect and income effect were expounded. Countermeasures and suggestions were put forward.

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Performance evaluation of river chief system in Changzhou city based on the perspective of ecosystem service

DONG Xiao-die

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 163-168. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.033

Abstract （374）

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From the perspective of ecosystem service value, this study interpreted the implementation target of the river chief system and constructed the effectiveness evaluation system of Changzhou city river chief system. Based on the relevant data of Changzhou city from 2009 to 2017, entropy method was adopted to determine the weight of each index, and TOPSIS model was used to define the closeness degree between index value and ideal solution as the implementation performance level of river chief system in each year. The results showed that the implementation of the river chief system played an active role in the governance of rivers and lakes in Changzhou city, but the system performance showed volatility. Suggestions were put forward from four aspects, such as system, assessment and evaluation, action and publicity.

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Research on the coupling and coordination development of technological innovation capability and performance：Take Shanxi province as an example

LAN Mei-na, PENG You-yuan, LIU Yu-long, SUN Ya-rong, ZHAN Ting-bian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 169-176. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.034

Abstract （351）

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The coupling and coordination of technological innovation capability and performance can effectively promote the high-quality development of technological innovation. According to the panel data of scientific and technological innovation in Shanxi province from 2008 to 2018, the entropy weight TOPSIS method was used to evaluate the technological innovation capability of Shanxi province, and the super-efficient DEA was used to evaluate the technological innovation performance of Shanxi province, and then the coupling and coordination of the two were analyzed. The results showed that the technological innovation capability of Shanxi province was at the “lagging” level and the performance was at the “catch-up” level. However, with the help of the performance level being superior to the capability level, the coupling coordination degree between them had been promoted from a slight imbalance to primary coordination. Finally,it puts forward suggestions such as the establishment of performance-driven technological innovation development model.

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Influence of online Q & A comment features on perceived usefulness

ZHAO Ya-nan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 177-181. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.035

Abstract （397）

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Based on the elaboration likelihood model and the real online Q & A comments of “ask everyone” in Taobao, the influence of the quality, emotional tendency, consistency, total amount and feedback quantity of Q & A comments on perceived usefulness was studed by using questionnaire survey and hierarchical regression analysis by SPSS 24.0. The results showed that central pathway and marginal pathway factors had significant positive effects on perceived usefulness, and the former had a greater impact. It was suggested that users perceived usefulness could be improved by setting up online Q & A comment templates, refining overall indicators and guiding consumers comments.

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Research on legal system of land management right circulation

ZHU Qiang, LI Xin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 182-186. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.036

Abstract （333）

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This study combs the relevant legal systems and local practices of the transfer of land management rights in China. Mainly aimed at the “Administrative measures for the transfer of rural land management rights”（revised draft for comment）. Combining with the newly revised contents of the “Rural land contract law”. Comparing with the content before the revision, it finds out what problems have been solved and what problems still exist. It analyzes the supplementary problems of the the subsequent normative documents issued by the local governments, and puts forward relevant suggestions for problems that still exist in combination with practice.

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Cooperativity analysis of Chinese agricultural diversified investment in the process of modernization

BAI Shan-wen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 187-189. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.037

Abstract （363）

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With the rapid development of the market economy, the process of agricultural modernization continues to advance, which has played a positive role in promoting Chinese modernization cause. Because capital is an extremely important factor of productivity, the most important point in the modernization of agricultural development is the increasing demand for capital, but in practice, the capital of the agricultural industry has been continuously transferred to non-agriculture. In recent years, Chinese governments at all levels have gradually increased their investment in the agricultural industry, but their investment structure and total investment still can not meet the growing demand for capital development, then hindered the development of agricultural industry in the process of modernization. Therefore, in the development process of agricultural modernization, it is necessary to coordinate the investment behaviors of different subjects, thereby enhancing the diversified investment cooperativity of modern agriculture and promoting the development and realization of agricultural modernization. This study focuses on the development process of agricultural modernization, analyzes the system and influencing factors of Chinese agricultural diversified investment cooperativity in the process of modernization, and proposes policy recommendations to promote the cooperativity of agricultural diversified investment.

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The performance of scientific and technology investment in China from the perspective of externality and endogencity： Based on the comparison between government and market

ZHAO Dang-ru, LIU Ling

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 190-195. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.038

Abstract （348）

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Based on A-shares listed micro data of companies manufacturing from 2016 to 2017, this study uses DEA-Tobit model to calculate the government investment in science and technology influence on the performance of enterprise investment in science and technology level, and considers the output of science and technology under the influence of negative externalities based on the provincial macro data of 30 provinces（cities, autonomous regions） in China from 2016 to 2017,and then uses improved unexpected SBM model to measure China’s 30 provinces（cities, autonomous regions） of investment in science and technology performance of the government and enterprises by eliminating the impact on enterprise investment in science and technology of government investment in science and technology endogenous. The results show that government investment in science and technology negatively regulates the relationship between enterprise investment in science and technology and its performance. The performance of government and market investment in science and technology is higher in the developed regions. The performance of enterprises in the eastern coastal areas with the highest level of economic and technological development is higher than that of the government. The performance of the government and the market in the central region with a weak level of economic and technological development is equal to that of the market. The western region government investment in science and technology performance is higher than the market. The environmental pollution caused by science and technology in the central region with weak economy is more than that in the eastern region with the best economic development and the western region with the weakest development, which is in line with the inverted “U”relationship between the level of science and technology development and environmental pollution.

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The establishment and practice of the cohesion loop mode of village industry development based on the polymerization and reconstruction：A case study of Gantou village, Lingbao city, Henan province

LI Bao-hua

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 196-203. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.039

Abstract （320）

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Firstly, the study combed the type of village industrial, and sumed up the three major embarrassment, development strategy of the three stages were formulated, included pre-positioning, medium-term construction and late development. Secondly, in time longitudinal and cross-dimensional analyed the factors affecting of village industries, based on the choice of polymerization and the reconstruction, a cohesive mode of industrial development was established. Finally, taking Gantou village, Xiyan township, Lingbao city, Henan province as an example, relevant contents development mode of industrial the cohesive ring model are applied practically.

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A study on the influencing factors of the sustainable livelihood of the shake off poverty households in the context of rural revitalization： A case study of the Qinba Mountain area

XIA Shi-han, WANG Jian-hong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 204-208. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.040

Abstract （348）

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Based on the household survey data of Qinba Mountain area, the factors affecting sustainable livelihood of shake off poverty households in this area were analyzed by principal component factor analysis. The results show that the livelihood level of shake off poverty households in Qinba Mountain area have improved significantly but the structure is not balanced. The livelihood of shake off poverty households are too dependent on nature, material capital and government investment. The investment in financial, human and social capital are relatively lacking, which need to be further strengthened. Finally, the corresponding countermeasures and suggestions are put forward.

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Government support, R&D investment and innovation speed of agricultural enterprises under rural revitalization strategy

ZHANG Yu-meng, MENG Qing-jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 209-212. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.041

Abstract （377）

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With the implementation of the rural revitalization strategy,solving the problems concerning “agriculture, rural areas and farmers” has become an important part of the party’s work. Agricultural enterprises as one of the key power to promote the development of agricultural economy, are faced with the important opportunities for innovation and development. Thus, it is very important to accelerate the innovation speed of agricultural enterprises at present. In this study, 14 agricultural companies listed on Shanghai and Shenzhen a-share markets from 2014 to 2018 are selected as research objects to test the influence between government support, R&D investment and innovation speed. The results show that government support is conducive to the increase of agricultural enterprises’ R&D investment,and R&D investment has a significant effect on promoting the innovation speed of agricultural enterprises. In addition, the combination of government support and R&D investment can greatly improve the innovation speed of agricultural enterprises.Therefore, in the context of the rural-revitalization strategy, the government should further increase the subsidy to agricultural enterprises, and at the same time, agricultural enterprises are supposed to constantly increase the investment in research and development, so as to enhance the competitiveness of enterprises, and achieve innovative development.

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Value analysis of Chinese traditional culture in helping rural revitalization

YIN Xing-chuang, WANG Xiao-xia

HUBEI AGRICULTURAL SCIENCES 2020, 59 (15): 213-216. DOI: 10.14088/j.cnki.issn0439-8114.2020.15.042

Abstract （562）

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Under the background of Rural Revitalization Strategy, the value of Chinese traditional culture in rural revitalization was analyzed, and the countermeasures and suggestions to give full play to the value of traditional culture and help rural revitalization were put forward.

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Research progress on nutrient effect of mineral elements on kiwifruit

LI Xiu-li, DAI Zhi-gang, CHEN Zhi-wei, CHEN Zhen, ZHAI Jing-hua, JI Xiao-mei, YUE You-zhang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 5-10. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.001

Abstract （444）

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The influences of mineral elements on soil nutrition, tree nutrition, fruit yield, quality and resistance of kiwifruit（Actinidia chinensis Planch） were summarized. Mineral elements mainly included macro elements （nitrogen, phosphorus, potassium）, middle elements （calcium, magnesium, sulfur）, trace elements （chlorine, boron, zinc, iron）. The interaction between nutrients was introduced, and the appropriate ranges of nutrient concentrations in kiwifruit leaves as well as in soil were also described. Several problems on nutrition of kiwifruit research were presented, including the nutrient demand in soil for different kiwifruit species and their accumulation characteristics of nutrients, the efficient utilization of nutrients, the interaction effects of nutrients and balanced fertilization, should be focused.

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Research progress of farmland abandonment process and influence factors at home and abroad

SHI Tie-chou

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 11-16. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.002

Abstract （554）

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Farmland abandonment occurred in Europe at the earliest, and gradually evolved into a global phenomenon of land use, it has profoundly changed the land landscape in the vast rural areas. The scale and process of farmland abandonment in foreign countries were reviewed, influencing factors of farmland abandonment in foreign countries, abandonment and agricultural land marginalization in China, and the existing problems in the study of farmland abandonment at home and abroad were summarized, and the research on China's farmland abandonment was prospected.

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Changes of total flavonoids in different organs of Fagopyrum tataricum during different growth period

ZHANG Yi-zhong, DENG Lin-qiong, FENG Tu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 17-19. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.003

Abstract （379）

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The changes of total flavonoids content in different organs of five Fagopyrum tataricum materials at different developmental stages were studied. The results showed that there were differences in the content of total flavonoids in different organs at the same developmental stage and in the same organs at different developmental stages. The order of total flavonoids content was leaf > stem > root in young leaf, flowering and fruit stages. Except for material TA181, there was no significant difference in total flavonoids content between stem and root in young leaf and fruit stages, the difference of total flavonoids content in leaves, stems and roots of other Fagopyrum tataricum reached significant level. Among the roots, stems, leaves, flowers and fruits, the content of total flavonoids in flowers was the highest, followed by leaves, and the lowest in roots. The order of total flavonoids content from big to small was flower > leaf > fruit > stem > root.

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Effects of salt stress on seed germination of five compositaes

ZHANG Zhi-hong, GU Kai-li, JI Jie

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 20-25. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.004

Abstract （562）

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The effects of NaCl salt stress on seed germination of were studied by using the seeds of five species of compositaes, such as Calendula officinalis L.,Coreopsis drummondii Torr. et Gray,Rudbeckia hirta L.,Gaillardia pulchella Foug. and Leucanthemum maximum （Ramood） DC. The results showed that the relative germination rate, relative germination potential and relative germination index of five chrysanthemum plants were significant negatively correlated with NaCl concentration. The high NaCl environmental concentration inhibited and even poisoned the germination of five species of chrysanthemum. Different species have different tolerance to salt in compositaes. The tolerance of the Gaillardia pulchella Foug. was the weakest, and the germination rate was 0 at 0.5% NaCl concentration.The NaCl concentration of 0.5% had a significant inhibitory effect on the germination of five kinds of compositaes. In production, 0.5% can be used as the concentration limit value of soil NaCl environmental solution for the germination of these plant seeds. All five species of chrysanthemum plants have certain salt-resistant nature. They can properly accept the lower NaCl concentration environment of 0.10%~0.20%, of which 0.1% of the NaCl concentration also plays a role in promoting seed germination of the Gaillardia pulchella Foug.

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Effects of different soil types on the content of copper and zinc in farmland

SONG Xiao-lan, LI Xiang, ZHENG Tong-yue, ZHAO Shan-li, DU Miao-miao, DING Jin-li

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 26-29. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.005

Abstract （407）

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Four typical farmland soils （alluvial soil, lime concretion black soil, yellow-cinnamon soil and cinnamon soil） were selected to analyze the distribution characteristics of Cu and Zn contents in farmland. Results showed that the Cu content in the surface of different soils was in order as followingYellow-cinnamon soil > lime concretion black soil > cinnamon soil > alluvial soil; In addition, Cu content in yellow-cinnamon soil was significantly different from that in other three soil types. The Zn content in the lime concretion black soil was as high as 225.40 mg/kg, which was significantly higher than that in the alluvial soil, yellow-cinnamon soil and cinnamon soil. According to the vertical distribution characteristics, the contents in the surface layer（0~20 cm） of Cu and Zn in the lime concretion black soil and yellow-cinnamon soil were significantly higher than that in the 20~40 cm and 40~60 cm soil layer. For the Cu content in alluvial soil, there was no significant difference between the soil layer of 0~20 cm and 40~60 cm. However, the Cu content of 0~20 cm for cinnamon soil was significantly different from that of 20~40 cm and 40~60 cm soil layers. The content of Zn in lime concretion black soil, yellow-cinnamon soil and cinnamon soil showed a trend of gradual increased with the increase of soil depth. The content of Cu and Zn in the four different soil types was lower than the national standard, which were in the stage of cleaning and still cleaning.

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Analysis and pollution risk assessment on heavy metal contents of farmland soils in Huangpi district of Wuhan city

WANG Su-ping, ZHANG Gui-you, DU Lei, HUANG Xiang, HONG Juan, JIANG Li, LIAN Zhi-cheng, ZHANG Li-hong, YE Li-xia, CHEN Gang, LI Pei-gen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 30-33. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.006

Abstract （362）

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Eight heavy metal elements （As,Cd,Cr,Cu,Ni,Pb,Zn,Hg） from 364 representative farmland soil surface samples were collected and analyzed in order to systematically understand the pollution level and ecological risk of heavy metals in the typical farmland soil in Huangpi district of Wuhan city. The single factor pollution index and Nemero comprehensive pollution index were used to evaluate the heavy metal pollution status of the farmland soil in this area. The results showed that more than 90% of the farmland soil in the area was in a clean level, and the main pollution was Cd pollution. 7.69% of the farmland soil was in the Cd pollution state, and less than 3.02% of the farmland soil was in the Cu and Ni pollution state. It was not found that the farmland soil was polluted by As, Cr, Pb, Hg and Zn elements.

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Study on the effect of polyhalite on wheat in yellow soil and purple soil in western Hubei province

WU Mao-qian, CHENG Zi-zhen, LIU Dong-bi, ZHOU Ji-wen, KONG Xiang-qiong, ZHANG Xiang-cheng, LI Yong-peng, ROBERT MEAKIN, FAN Xiao-hui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 34-37. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.007

Abstract （476）

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The effect of polyhalite on wheat in yellow and purple soils collected from west Hubei province was studied by a greenhouse pot experiment in whole wheat growth duration. Results showed that, in yellow and purple soils, polyhalite treatment significantly increased grain yield of wheat by 28.7% and 46.6% respectively compared with CK （KCl） treatment, which mainly because polyhalite treatment significantly increased the amount of effective panicle by 31.2% and 37.5% in yellow and purple soils, respectively. In yellow soil, Ca and Mg uptake in wheat plant from polyhalite treatment was almost identical to that from CK treatment, whereas S uptake from polyhalite treatment was significantly higher than that from CK treatment by 44.6%; In purple soil, polyhalite treatment significantly increased Ca, Mg and S uptake in wheat plant by 7.7%, 25.0% and 81.8%, respectively. The above results implied that, wheat responded better to polyhalite in purple soil than in yellow soil, and mainly because the effective Ca, Mg and S content in purple soil was obviously lower than that in yellow soil.

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Effects of soil modifiers on physical and chemical properties of green orange garden soil and growth of green orange seedlings

WU Yu-jia, JI Qing-mei, LEI Fei, ZHANG Dong-ming, YANG Chun, FU Chuan-liang, TAN Hao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 38-41. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.008

Abstract （394）

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Green orange garden soil and green orange seedling were used as materials, and different soil modifiers were used to carry out pot experiment. The results showed that the application of organic fertilizer and soil modifiers could improve the pH, available nutrients and soil structure of green orange garden soil. It can also promote the growth of green orange seedlings. Through the contrast test among the treatments, it was found that the effect of organic fertilizer （2‰） + hydrated lime （1‰） and organic fertilizer （2‰） + calcium magnesium phosphate （1‰） were the best. Compared with the control, the pH of soil increased by 1.23 and 0.82, the exchangeable aluminum content of soil decreased by 97.65% and 96.86%, respectively, the percentage of soil aggregate structure increased by more than 1.52%, and the dry weight of aboveground part of green orange seedling was higher than that of the control by more than 40.09%.

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Effects of the fungi chaff of pleurotus geesteranus on vegetable and soil quality in greenhouse

JI Qing-mei, WU Yu-jia, ZANG Dong-ming, LEI Fei, ZENG Jian-hua, FU Chuan-liang, ZHANG Wen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 42-48. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.009

Abstract （369）

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The experiment of greenhouse vegetables was carried out with the materials of Chinese cabbage named snow honey, Chinese cabbage named black leaves and the fungi chaff of pleurotus geesteranus. Different application rates of fungi chaff and reduced fertilizer were set to research the effects that different contents of fungi chaff of one crop on the growth, quality and soil of three consecutive crops of vegetables. The results showed that using fungi chaff instead of chemical fertilizer N can increase the yield and quality of greenhouse vegetables and improve soil quality in a range of 20%~40% and have a certain continuation effect. The best results obtained by using the mixture of fungi chaff, sheep manure and inorganic fertilizer was that vegetables growed well, such as plant height and stem diameter were the largest, high yield and high vitamin C content, its nitrate content was the lowest or the second low. After the first harvest, compared with the control, the soil pH of the two vegetables increased by 0.18 and 0.17, the organic matter increased by 6.06% and 11.19%, the alkali-hydrolyzed nitrogen increased by -1.49% and 5.08%, the available phosphorus increased by 7.73% and 4.40%, and the available potassium decreased by 1.69% and 1.99%. The yield increases of the three consecutivecrops of the Chinese cabbage named snow honey were 14.5%, 10.5% and 5.8%, respectively, the Chinese cabbage named black leaves were 12.1%, 10.5% and 7.1%, respectively.

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Empirical analysis of the impact of carbon reduction policies on carbon emissions：Based on STIRPAT expansion model

LIU Ling, LI Xiao-jun, PENG Jian-bo

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 49-53. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.010

Abstract （472）

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Panel data of 30 provinces （except Tibet, Hong Kong, Macao and Taiwan） from 2007 to 2017 were used to introduce the carbon emission reduction policy into STIRPAT model, and empirical analysis was made on the policy binding force and timeliness of carbon emission reduction policy. The results showed that for the whole country, the direct binding force and timeliness of carbon emission reduction policies were limited, while for the region, the long-term binding force and timeliness of carbon emission reduction policies in the western region were higher than those in the central and eastern regions. Finally, the study puts forward some suggestions on differentiated carbon reduction policy according to the regional impact differences of carbon reduction policies.

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Land cover change analysis and trend prediction by the GM（1,1） model in Changzhutan region

GUO Rong-zhong, SHEN Hai-jian, YANG Min-hua

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 54-60. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.011

Abstract （358）

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The land cover changes and the land use intensity in Changzhutan area from 2002 to 2014 were evaluated quantitatively, and its changed trends were predicted and analyzed by the GM（1,1） model. The results showed that great changes were observed in various types of land uses in the Changzhutan area from 2002 to 2014, including a net increase in the area of urban and rural land, industrial and mining land, and transportation land, and a net decrease in the area of other types of land. In the same period, the land reclamation index in Changzhutan area decreased year by year. The land development intensity index, land utilization rate index and land utilization intensity composite index all showed an increasing trend year by year. At the same time, various types of land use in the studied areas from 2017 to 2026 are predicted that unused land, gardens, grasslands, wetlands, arable land, woodlands and water areas would be to some extent reduced with the change rates about -19.15%, -12.31%, -7.10%, -3.12%, -2.80%, -1.18%, respectively. However, the areas of land for transportation, residential areas and industrial and mining land have been increased with the change rates of 35.57% and 15.61%, respectively.

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Analysis on the characteristics and effects of continuous rain disaster in Hubei province

ZHENG Zhi-bin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 61-65. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.012

Abstract （425）

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Based on the daily precipitation data monitored by the national surface meteorological observation station of Hubei province in spring and autumn from 1961 to 2017, the characteristics and effects of continuous rain disasters in Hubei province are analyzed, so as to reduce the impact on production and life. The results show that the occurrence frequency of continuous rain in spring in Hubei province is higher in the south than in the north while in autumn is more in the west than in the east,and more in the south than in the north,most in the southwest of Hubei. Since entering the 21st century, the occurrence area and degree have reduced in spring, but since 2010, the occurrence scope and degree show an increasing trend in autumn; The convergence of cold and warm air in Hubei province is the main cause of continuous rain; Continuous rain not only causes heavy losses to agricultural production, but also causes harm to relevant production activities in the storage and transportation industry, and also affects people's physical and mental health, so corresponding defensive measures should be taken.

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Climate change characteristics analysis of Jingzhou city from 1961 to 2018

GAO Hua-dong, DENG Yan-jun, FEI Qiang, YANG Qing-qing, LIU Shi-hui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 66-71. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.013

Abstract （557）

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In order to study the temporal and spatial variation characteristics of climate in Jingzhou city, the data of average daily temperature, daily precipitation and sunshine duration from 1961 to 2018 of six national meteorological stations in Jingzhou city were used to analyze the temporal and spatial variation characteristics of major climatic factors in Jingzhou city by using linear tendency estimation method, cumulative anomaly method and M-K mutation test method. The results showed that in the past 58 years, the trend of temperature increase in Jingzhou city, especially since the 1990s; The precipitation showed upward trend, while the annual fluctuation was large; The annual solar duration decreased significantly. The temperature increase of Jingzhou city was mainly from February to May and from September to December. The precipitation increase trend was obvious in July, and the precipitation decrease was mainly in autumn; The decrease of sunshine duration in summer was the main reason for the poor illumination in Jingzhou city. The trend of climate change in stations Jingzhou city was basically the same, only the annual precipitation of Songzi station showed a decreasing trend, which was contrary to the overall change trend of Jingzhou city. With the warming of the climate, the heat resources of Jingzhou city increased obviously.

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Preliminary study on mildew mechanism of peanut seeds

YANG Shu-xiang, LIU Jin-xuan, JIANG Yang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 72-75. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.014

Abstract （359）

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According to the temperature and humidity characteristics of four seasons in Wuhan city, four different temperatures （8, 18, 28, 35 ℃） and air relative humidity （55%, 75%, 85%, 90%） were set to study the mould of peanut and calculate the mould rate. Two strains were isolated and inoculated from moldy peanut by dilution plate method. The results showed that the mould rate of peanut seeds reached 80.0% under the condition of 90% humidity and temperature of 28 ℃. It was preliminarily identified as Aspergillus flavus and Aspergillus niger by morphological observation and microscope.

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Experiment on Spodoptera frugiperda control with a mixture of insecticide and spray additives

ZHOU Hai-liang, MA Xue-lin, SONG Guo-feng, ZHANG Shu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 76-77. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.015

Abstract （298）

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The control experiments were carried out by using emamectin benzoate-indoxacarb 9%SC、emamectin benzoate 5%WG and emamectin benzoate 5%WG+pesticide adjuvant. The results showed that the treatment control effects of emamectin benzoate 5%WG+pesticide adjuvant was significantly higher than other treatments. The control effects 1, 3 and 7 days after treatment was 83.2%, 91.3% and 93.4%, respectively.

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The effect of four kinds of medium types on Verticillium dahliae V991b and its mutant

HUANG Wei, YUAN Bin, JIN Li-rong, YANG Xiao-lin, ZHANG Rui-yang, WAN Peng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 78-84. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.016

Abstract （381）

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In order to understand the biological characteristics and pathogenicity of Verticillium dahilae V991b and its mutant V991w, the effects of different conditioned medium on the growth and pathogenicity of the two pathogens were investigated. The results showed that the growth curve of two strains on different types of medium was linear basically. The growth rate of V991b on PDA was significantly different from other types of medium. And the growth rate of V991w on PDA and PCA medium was significantly different from other two types of medium. After cultiviated 15 days on PDA medium, there was no significantly difference between the colony diameter of V991b and that of V991w. During amplified the fungi in Czapek’s liquid medium and then inoculated the cotton seedling, the pathogenicity of V991b and V991w was quite different. The disease index on 25 days of inoculated was 56.63 and 23.23, respectively. However, the disease index of cotton seedlings inoculated with Czapek’s-cotton leaf tissue liquid medium was significantly reduced, and the disease index of V991b and V991w at 25 d inoculation was only 12.12 and 7.32. The plant tissue culture medium had no significant effect on the diameter of the microsclerotia, but it had significant inhibitory effect on the number of microsclerotia and the growth of hypha.

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Synthesis and fungicidal activity of N-alkylated glycine ethyl ester-phenazine-1-carboxylic acid derivatives

XIONG Yong-tong, HU Jin-yu, WANG Yun-ping, WU Qing-lai, LI Jun-kai

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 85-87. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.017

Abstract （317）

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In order to obtain highly active compounds, six novel N-alkylated glycine ethyl ester-phenazine-1-carboxylic acid derivatives were designed and synthesized using phenazine-1-carboxylic acid as a lead compound. The target compounds were characterized by ¹H NMR and HIGH resolution MASS spectrometry. The fungicidal activity of the target compounds was tested. The results showed that the synthesized target compounds had certain fungicidal activity against the six tested fungi.

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Introduction test of three different banana varieties in Huangzhou district

YAN Liang, CHEN Zhan-peng, JIANG Yan-yan, GE Chang-jun, XU Li-rong, DAI Jun-fen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 88-90. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.018

Abstract （1142）

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Three different banana varieties were introduced, two transplanting periods were set, the adaptability of different banana varieties was discussed through the analysis of their morphological characteristics, leaf growth dynamics, yield related characters and suitability in Huangzhou district of Huanggang city of Hubei province. The results showed that the suitable transplanting period of banana variety A was before June 18;the suitable transplanting period of banana variety C was before August 26, and this variety banana was the early ripe banana variety, the growth period was about 12 months; the suitable transplanting time of banana variety B in Huangzhou district was yet to be determined by further studies. In Huangzhou district, from April to June and from October to November were the suitable period for banana growth. Therefore, in banana introduction and cultivation management, some agricultural practices such as fertilization can be carried out in advance to improve the yield and quality of banana.

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Effects of sawdust from cinnamon processing residue on the growth of Ganoderma lucidum

ZHAN Nian-yong, LU Rong-sheng, DENG Li-jie, TANG Xuan, HAN Mei-li, LU Tai-rong, CHEN Jin-ning

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 91-95. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.019

Abstract （455）

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The effects of sawdust from cinnamon processing residue on the growth of Ganoderma lucidum were studied. The results showed that the growth rate of Ganoderma lucidum could be promoted by adding sawdust from cinnamon residue sawdust in different growth stages. The growth rate of mycelia could reach to 8.2~8.4 mm/d and the storage time of strain could be extended to 9 months when adding 75~150 g/L of cinnamon processing residue sawdust to the gar medium. The growth rate of mycelia could reach 8.4 mm/d and 9.3 mm/d when adding 200~300 g/kg and 300~500 g /kg of cinnamon processing residue sawdust to the primary seed medium and culture medium respectively, obvious higher than that of the control. Adding 450~600 g/kg of cinnamon processing residues sawdust and 75~100 g/kg of clay soil to medium for fruiting of Ganoderma lucidum could increase the biological conversion rate to 66%~72%.

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Effects of organic fertilizer on the composition and quality of tobacco leaves

YANG Zi-jun, SHEN Hong-tao, XU Chen-sheng, MA Shu-hao, ZHAO Peng-li, LI Guang-zhao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 96-99. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.020

Abstract （381）

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In order to study the effects of different kinds of organic fertilizer on the composition and quality of flue-cured tobacco, five treatments（1 500 kg/hm²） were used in the field experiment,included rapeseed cake fertilizer （T1）, sesame cake fertilizer （T2）, earthworm manure （T3）, high carbon base fertilizer （T4） and chemical fertilizer control （9N∶9P∶27K）（T5）. The effects of fertilizer types on the chemical composition and quality evaluation of flue-cured tobacco were studied. The results showed that the application of rapeseed cake fertilizer, sesame cake fertilizer, earthworm manure and high-carbon base fertilizer could affect the content of components and the results of smoking evaluation in the K326 tobacco planting area of Shaowu city. There were many factors that affected the content of components and smoking evaluation of the flue-cured tobacco. Taking permeability index as an example, sesame cake fertilizer treatment had the best penetrability, aroma quality and the lowest total nitrogen content; permeability was related to aroma quality, reducing sugar, sugar alkali ratio and nitrogen and potassium content. Rapeseed cake fertilizer had the best permeability to C3F tobacco, which was related to potassium content. 1 500 kg/hm² sesame cake fertilizer or rapeseed cake fertilizer could comprehensively improve the permeability of tobacco leaves.

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Discovery and research of new distribution species of endangered plants Primula mallophylla in Hubei province

YANG Lin-sen, PENG Jin-long, LI Hong-tao, WANG Jing, XIE Xiao-lin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 100-101. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.021

Abstract （415）

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Primula mallophylla, a new species of Primula in Primulaceae from Hubei province, was found in Shennongjia, western Hubei. Supplementary descriptions were made according to the specimens. The habitat characteristics of this species were described. The causes of endangerment of this extremely endangered species were preliminarily analyzed, and suggestions for strengthening protection were put forward.

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Effects of different water quality on main physicochemical composition and quality of Yinghong No.9 black tea infusion

ZHOU Qiao-yi, LING Cai-jin, LIN Wei-peng, LIU Shu-mei, GUAN Jie-ting, GAO Li-yang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 102-107. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.022

Abstract （466）

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Yinghong No.9 black tea was chosen as test material for investigation of the color, pH, electrical conductivity and main quality components of tea infusion when five types water （ultra pure water, tap water, natural mountain spring water, natural mineral water, purified water） were applied. Through correlation analysis, the effects of different water quality on the color and quality components of Yinghong No.9 black tea soup were studied. The results showed that pH, conductivity and soluble solids content of water samples increased, brightness of tea infusion decreased and redness increased, different water quality had significant influence on the total catechins, especially ester catechin.

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The preliminary adaptability of 7 tea plant cultivars in Guilin

ZHANG Ling-yun, SU Min, QIN Yu-long, JIANG Chang-jian, LI Shu-yi, LI Zhuo-yi, QING Yan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 108-111. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.023

Abstract （475）

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Taking Fudingdabai as the control,two years comprehensive adaptability evaluation were carried out about Zhonghuang No.1, Longjingchangye,Longjing 43,Huangjinya, Cuiyu, Zhongbai No. 1, Hongyan No.12 in Guilin city. The results showed that the general performance of,Longjing 43,Longjingchangye, Zhonghuang No.1,Huangjinya, Cuiyu,Hongyan No.12 were exceeded or closed to the control in the growth potential, phenological period, processing quality, and resistance,which were suitable for planting in tea-producing areas of Guilin. Among them, Longjing 43 had the best comprehensive performance, which was mainly reflected in its strong adaptability, high transplanting survival rate and excellent tea quality. However, Zhongbai No.1 was unsuitable for tea planting in tea-producing areas of Guilin.

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Effect of forage sweet sorghum silage instead of silage corn on nutrient digestibility, body weight gain of lambs

WANG Hong-bo, LI Wei-hong, YANG Xiao-ling, GAO Ya-qin, XI Bin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 112-114. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.024

Abstract （407）

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In order to study the effect of forage sweet sorghum silage instead of silage corn on lamb performance and nutrient digestion and metabolism. Thirty 3-month-old （28.10±0.32 kg） healthy weaned lambs were randomly divided into three groups （I was corn silage group, II was sweet sorghum corn silage group, III was sweet sorghum silage group）, each group had 10 lambs, and were fed and digested separately. The results showed that there was no significant effect on the performance of lambs （P>0.05）. The apparent digestibility of group Ⅱ was significantly higher than that of group Ⅲ （P<0.01）, while the different between that of groupⅠand group Ⅱ was not significant （P>0.05）. Lamb had no significant effect on the apparent digestibility of other nutrients in corn silage and 50% sweet sorghum silage instead of 50% corn silage （P>0.05）, but had no significant effect on other nutrients. The results showed that sweet sorghum silage can be used as high quality feed for meat sheep, and 50% of sweet sorghum straw and silage can replace corn silage respectively, which can improve the N retention rate and CP digestibility of lamb.

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Diagnosis of mixed infection of Riemerella anatipestifer with duck paramyxovirus disease

LUO Ling, LI Li, WANG Hong-cai, WEN Guo-yuan, LUO Qing-ping, WANG Hong-lin, LU Qin, SHANG Yu, SHAO Hua-bin, WANG Xiao-zhong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 115-117. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.025

Abstract （378）

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：In July 2019, it happened the outbreak of disease and caused a lot of death on a farm of 10 000 ducks in Hubei province.Based on laboratory bacteriological diagnosis and virological diagnosis, the results showed the ducks infected with Riemerella anatipestifer and paramyxovirus.

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Isolation, identification and drug resistance analysis of Staphylococci in the air of chicken houses

LU Yuan-qing, ZHANG Teng-fei, SHAO Hua-bin, ZENG Chi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 118-121. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.026

Abstract （306）

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Coagulase-negative staphylococci （CoNS） are a group of opportunistic pathogens, which are widely spread in the environment. Animal breeding is an important source of pathogen spreading. However, the prevalence and characteristics of CoNS in the bioaerosols of poultry houses are unclear. In this study, through further isolation and identification of bacteria collected from bioaerosols in poultry farms, 102 strains of coagulase-negative staphylococci were collected. Among these isolates, 41.18% isolates were Staphylococcus sciuri, which was the predominant species, followed by S. equorum （21.57%）, S. saprophyticus （18.63%）, S. haemolyticus （10.78%） and S. xylosus （5.88%）. Resistance analysis showed that high resistance rates to penicillin, ampicillin, ciprofloxacin, chloramphenicol, erythromycin, tetracycline, clindamycin and oxacillin among these staphylococci. The presence of mecA was screened by PCR in all CoNS, and it showed that 36.3% isolates contained resistance gene mecA and were resistance to oxacillin, and mecA is a marker resistance gene of methicillin-resistant S. aureus （MRSA）.

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Study on the effectiveness of sensory quality evaluation of paper-process reconstituted tobacco

WANG Yan-hong, ZHOU Gui-yuan, TU Yan-gang, ZHANG Xiang-hui, ZHANG Wen-jun, WANG Xiao-sheng, ZHANG Yan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 122-125. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.027

Abstract （366）

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Usingthe cigarettewhich made by reconstituted tobacco as the research object, based on the characteristics of reconstituted tobacco and the method ofattributeconsistency, the sensory quality of paper-made tobacco leaves was evaluated from the aspects of aroma characteristic, coordination, smoke characteristic, taste characteristic and combustion characteristic, and the evaluation data was compared with the standard score, and the results were analyzed for attribute consistency. The results showed thatthe Kappa value was more than 0.70. The sensory quality of paper-process reconstituted tobacco leaves was stable and consistent with experts, and the sensory evaluation data was effective. The method was accurate, simple and easy to operate.

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Optimization of extraction technology of total flavonoids from Chinese medicine Schisandra propinqua

TANG Chun-li, WEI Jiang-cun, LU Mei-yuan, XIE Zhen, CHEN Hong-xia, ZHAO Feng-xian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 126-129. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.028

Abstract （336）

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The total flavonoids of Schisandra propinqua were extracted by reflux extraction method. The effects of extraction method, extraction solvent volume fraction, extraction volume and extraction time on the total flavonoids content of the medicinal materials were investigated by single factor experiment. The orthogonal test was used to optimize the extraction conditions. The best extraction process for a total flavonoid of Schisandra propinqua was screened out. The results showed that the optimal extraction process of a total flavonoid of Schisandra propinqua was 70% methanol, the ratio of material to liquid was 1∶40（m∶V）, and the reflux extraction time was 1.0 h. This method could effectively determine the content of total flavonoids in Schisandra propinqua medicinal material, the repeatability was good, the method was stable and feasible.

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Identification and mutation breeding of a halophilic strain with high α-amylase-producing activity

ZHANG Wei, WU Xiao-wei, YAO Yan-hong, ZHANG Fen-fen, ZHOU Xiao-lun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 130-133. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.029

Abstract （459）

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A halophilic strain NWU-8 that produced α-amylase was isolated and screened from the soil. Through morphological, physiological and biochemical and 16S rRNA gene sequence analysis, the strain NWU-8 was identified as Halorubrum sp., and the initial amylase activity was 32 U/mL. In order to improve the ability of the strain to produce α-amylase, the strain NWU-8 was mutated by He-Ne irradiation. After the mutation, the mutant NWU-8-14 produced alpha-amylase activity up to 138 U/mL, which was 331% higher than the wild strain. In addition, when the salt concentration was as high as 35%, its α-amylase activity still maintained 80 U/mL. NWU-8-14 was a kind of salt phages with high amylase production. This mutant strain had broad application prospects in food fermentation and environmental protection.

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Comparison on infrared spectrum, thermal properties and microstructure between edible bird’s nest and tremella

DING Rong, WU Qiu-di, ZOU Yin-bo, LI Mei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 134-138. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.030

Abstract （471）

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In order to distinguish the chemical structure, thermal properties and microstructure between edible bird’s nest and tremella, the chemical functional groups of edible bird’s nest and tremella were analyzed with infrared spectrum （FTIR）, the first and the second derivatives as well as Norris first derivative of FTIR were studied, the thermal properties were analyzed with comprehensive thermal analyzer, and their surface microstructure were observed with SEM. The results showed that the main absorption position of infrared spectrum of edible bird’s nest was close to tremella while the absorption peak intensity are fairly different with that of edible bird’s nest being stronger. The absorption peaks of protein, amino acid, and the hydroxyl, methyl, and methylene of polysaccharide were observed at 3 431 and 2 922 cm^-1. The protein amide I band, II band, and III band stretching absorption peak were observed at 1 636, 1 448, 1 384 cm^-1. However, tremella had absorption peak of fat around 1 732 cm^-1. In the fingerprint region with a wave number less than 1 300 cm^-1, the first and the second derivatives and the Norris first derivative of edible bird’s nest and tremella showed great differences. Thermal weight loss curves and DSC curves betweenedible bird’s nest and tremella were significantly different. Thermal weight loss of bird’s nest was greater at 160~400 ℃, while that of tremella was greater at 250~400 ℃. There are distinct differences on microstructure ofedible bird’s nest and tremella. Obvious strip texture oriented in a certain direction can be seen on the surface of edible bird’s nest while considerable convex body accumulated on the surface of tremella. The results provided the basis to distinguish edible bird’s nest and tremella in spectral analysis and microstructure using integrated approaches.

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Study on quality analysis method of konjac

YIN Gui-fang, SUN Dao-wang, LU Wen-jie, WANG Yan-qing, WANG Ling, WANG Li-hua

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 139-141. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.031

Abstract （341）

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In order to reduce breeding cost and shorten breeding years, the quality of konjac was analyzed by means of oven baking a few samples and small pulverizer grinding. The results showed that only 600 g fresh konjac could accurately measure the water content, powder yield and viscosity of a sample, absolute deviation of moisture content measurement value was within 0.3%, absolute deviation of powder yield measurement value was within 0.16%, the absolute deviation of the determination value of the viscosity of fine powder was far less than that of the secondary independent determination result specified in the national standard for agricultural industry NY/T494—2010, which was less than 1 000 mPa.s. This method provided an important reference for the selection and breeding of konjac superior varieties, and laid a foundation for accelerating the breeding process of konjac.

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Evaluation of uncertainty in the determination of cadmium in food by ICP-MS

ZHOU Ling, WAN Chun-yan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 142-144. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.032

Abstract （348）

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According to the JJF1059.1—2012 standard, the measurement model was established to determine and calculate the uncertainty components in the determination process, and analyze the uncertainty in the determination of cadmium in foods by ICP-MS. The results showed that the main influence factors of uncertainty were the calibration of the standard curve fitted by least square method, the reproducibility of measurement and the standard recovery. In order to improve the accuracy of the measurement results, the control of these aspects should be strengthened.

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Research and development of the embedded intelligent maintenance management system of agricultural tools

LIU Kun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 145-148. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.033

Abstract （375）

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In the agricultural tool maintenance process, it was necessary to ensure that agricultural maintenance tools are not missed or lost. At present, it was inefficient, wasted manpower and material resources that the manual supervision method was generally adopted. The intelligent management system for agricultural maintenance tools was studied and developed to solve problems of airport maintenance. With various technologies of the Internet of things, RFID tags were attached on the surface of maintenance tools, and tool management information was obtained by scanning and identifying the tags on the maintenance tools.

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Design and test of hole-type seed meter

LU Wei-an, HUANG Shi-ming, FENG Tian-yu, CHENG Jie, HAO Wen-bin, WANG Ben-yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 149-152. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.034

Abstract （517）

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The seed meter is the core component of the rice direct seeder,and its performance directly affects the operation of the rice drill. Firstly,selected the type of seeder by briefly describing the type of seeder,and calculated the main parameters of the device. Secondly,the stress generated by the seed meter in the working process was analyzed. Finally,the bench test was carried out. The rationality of seed meter design was verified. The results showed that the design of this device conforms to the agronomic requirements of rice direct seeding.

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Research on identification of agricultural insects based on depth residual network with multi-feature and multi-granularity

LI Yan-hong, FAN Tong-ke

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 153-157. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.035

Abstract （297）

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In order to realize insect identification under complex farmland background, this paper proposed a multi-feature and multi-granularity insect identification method based on deep residual network. Compared with the traditional SVM and BP neural network, the accuracy of insect identification based on deep residual network was significantly improved in complex farmland background. Compared with the deep convolutional neural network such as AlexNet, the performance of our method was further improved after the depth residual learning optimization. And the accuracy of 98.67% was obtained on the classification of crop insect images under the background of 10 types of complex farmland. Therefore, this method has high practical application value and can be integrated into the actual agricultural insect control task with the currently used agricultural networking system.

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Analysis and prediction model of meteorological restriction factors of Anhui province rapeseed single yield based on decision tree algorithm

YANG Xiao-bing, YANG Chen, REN Zhong, YANG Jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 158-160. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.036

Abstract （318）

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Aiming to construct a local rapeseed single yield forecasting model based on analysis of the influence of meteorological factors on rapeseed yield in Anhui region, the datas containing surface meteorological data of 78 observation sites in Anhui province from 1999 to 2018 and rapeseed single yield of 78 counties （cities, districts） from 2000 to 2018 were collected. The decision tree algorithm was adopted to analyze influence of meteorological factors on rapeseed output which finally screened out the main factors. A predictive model of rapeseed output based on support vector machine （SVM） was constructed. The results showed that the main meteorological factors which affected rapeseed yield were wetting index during maturity, average temperature during bud stage, wetting index at seedling stage and flowering stage. The root mean square error between predicted and measured value was 402 kg/hm², and fitting index was 0.72 which proved the prediction model behaves well on the whole.

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Analysis on the integration of professional work and financial management in provincial agricultural sciences research units under the new situation

XU E, CHEN Jie, LI Kui, HU Die

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 161-165. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.037

Abstract （364）

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The problems existing in the financial management of the current provincial agricultural sciences research units were analyzed. Under the new situation of scientific and technological innovation and the reform of “decentralization, management and service”, the importance of the integration of professional work and financial management in provincial agricultural sciences research units has been clarified, and the difficulties and obstacles of the integration of professional work and financial management have also been discussed. From the aspects of strengthening financial management system innovation,enhancing the awareness of the integration of professional work and finance management, strengthening informatization construction, innovating horizontal scientific research project funding management, setting up scientific research financial assistant positions, and improving the assessment and incentive mechanism, countermeasures and suggestions have been put forward to promote the integration of professional work and financial management in provincial agricultural sciences research units.

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Research on tourism image of the Song Dynasty of Kungfu city based on network text analysis

GUO Meng-di, TIAN Yu-hui, SUN Li-qiang, WANG Peng-fei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 166-171. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.038

Abstract （477）

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Take the Song Dynasty of Kungfu city in Kaifeng city of Henan province as an example,through websites such as Ctrip.com,LY.com and Mafengwo.com, the related comments and travel notes of the scenic spot were obtained. By using the network text analysis method and the Rost Content Mining software, the high-frequency feature words analysis, semantic network analysis and emotional analysis of the text were carried out,so as to study the tourists' perception of the tourism image of the scenic spot. The results showed that, tourists have a deep perception of the cultural theme characteristics of the Song Dynasty of Kungfu city, and have high satisfaction with the tourism activities. The emotional attitude was mainly positive, while negative emotions mainly focus on the facilities and services of the scenic spot. Therefore,the scenic spot should strengthen cultural publicity, and focus on improving the quality of facilities and services in scenic spot, so as to further enhance the tourism image of the scenic spot.

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Evaluation and analysis of the development efficiency of circular agriculture：Take Mianyang city in Sichuan province as an example

TANG Di, CHEN Chang-hong, PU Yang-hua

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 172-175. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.039

Abstract （411）

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Developing circular agriculture was an important way to promote agricultural modernization. Through the evaluation and analysis of the development efficiency of circular agriculture in Mianyang city of Sichuan province, it can effectively promote the development of local circular agriculture and realize green development while realizing the transformation of agricultural modernization. The CCR model of DEA methods was used to evaluate the development efficiency of circular agriculture in Mianyang city. The results showed that in 2013, 2014, 2015 and 2017, the index efficiency of circular agriculture in Mianyang city reached the relative optimal, and DEA was effective; but in 2016, the index efficiency did not reach the optimal, and DEA was invalid. Finally, some suggestions on how to adjust the development efficiency of circular agriculture in Mianyang city were put forward.

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Evaluation of the implementation effect of general land use planning based on GIS:Take Haian county as an example

YAO Yi, LIU Fu-cheng, LU Xia

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 176-180. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.040

Abstract （312）

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The general land use planning was a macro-strategic measure to coordinate the development, utilization and protection of regional land resources and promote the coordinated development of regional economy, society and environment. Evaluation of the implementation effect of general land use planning was an important way to supervise the implementation process of land use planning and improve the utilization efficiency of land resources. Based on the data of the second land survey of Haian county in 2009, the data of land use status in 2014 and the data of land use planning in 2020, the implementation effect of the general land use planning of Haian county was evaluated. The results had reference value for the revision of land use planning and the optimization of land use direction of Haian county.

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Study on sustainable development of Yellow River ecological belt：Take eight cities along the Yellow River in Henan province as an example

FU Biao, TANG Zheng-qing, WANG Xing, YAO Ming-feng, ZHANG Xiang-feng, WANG Dong-feng, WEN Chang-you

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 181-183. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.041

Abstract （481）

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The Yellow River ecological belt was an important way to practice the concept of green development, an important carrier to carry out the rural revitalization strategy, and an important area to promote the overall development of urban and rural areas, which was of great significance and far-reaching impact. Taking eight cities along the Yellow River in Henan province as an example,this study showed that,the Yellow River ecological belt basin was short of water resources, the ecological environment was fragile, and the task of poverty alleviation and the construction of floodplain were arduous. The suggestions are put forward for the sustainable development of the Yellow River ecological belt,such as promoting the construction of the Yellow River ecological belt as a national strategy, carrying out pilot demonstrations for the coordinated development of ecological civilization in river basin, supporting the protection and development of the Yellow River floodplain, and planning the construction of the core demonstration zone for the Yellow River ecological belt. It provided new ideas and methods for the construction of the Yellow River ecological belt.

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Solving the problems and countermeasures of Mengzi pomegranate sales based on e-commerce mode

DING Peng-li, TAN Shao-jie, WANG Chang-mei, ZHANG Wu-di, YIN Fang, ZHAO Xing-ling, WU Kai, LIU Jing, YANG Hong, LIU Shi-qing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 184-187. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.042

Abstract （452）

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In recent years, under the background of “Internet plus” rapid development, Mengzi city in Yunnan province took e-commerce into rural comprehensive demonstration project as a breakthrough,and comprehensively promoted and encouraged public entrepreneurship and innovation. However, due to the lack of talents,technologyand other factors,there were some difficulties in the development of rural e-commerce in Mengzi. This study analyzed the current situation of Mengzi pomegranate e-commerce development, found out the problems existing in the development of Mengzi pomegranate e-commerce through field visits, access to information and other methods, and put forward the corresponding countermeasures and suggestions.

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Evaluation and development of ecotourism resources in Niubeiliang forest park

WANG Xin-xin, WANG Yi, ZHANG Xue, LI Rong-rong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 188-192. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.043

Abstract （386）

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With the improvement of living standards, people’s spiritual needs were increasing. With the rapid development of tourism, ecotourism was playing a more and more important role in the tourism industry. However, the level of ecotourism resources in each scenicspot was different, and the development status was also different. Taking Niubeiliang forest park as an example, this study comprehensively evaluated the ecotourism resources of the park and analyzes its development status. According to the results of ecotourism resources evaluation and development status analysis, the corresponding development countermeasures were put forward to promote the further development of Niubeiliang forest park.

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Analysis on the willingness and influencing factors of farmers' participation in livestock and poultry care in poverty-stricken areas：Investigation based on Hotan city in Xinjiang

CUI Yu-hang, GUAN Zhi-qiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 193-196. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.044

Abstract （453）

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Livestock and poultry care was a pioneering work in the targeted poverty alleviation work in the poor areas of southern Xinjiang. Based on the relevant survey data of Hotan city in Xinjiang, Logit model was used to analyze the willingness and influencing factors of farmers participation in livestock and poultry care. The results showed that the influence of age, family labor force, single breed and policy compliance on their willingness to participate in livestock and poultry care was not significant. Gender, education level, participation in breeding technology training, understanding of livestock and poultry care, quantity of livestock and poultry, breeding history, and government guidance had a significant impact on farmers’ willingness to participate in livestock and poultry care. Finally, put forward some countermeasures and suggestions to improved the willingness of farmers’ participation in livestock and poultry care.

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Evalution on the comprehensive inheritance degree of rural red culture in Shangluo city

ZHU Bai, YANG Zeng-qiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 197-202. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.045

Abstract （368）

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Taking Beikuanping town in Shangluo city of Shaanxi province as an example, the evaluation index of comprehensive inheritance degree of red culture in Beikuanping town was obtained by applying grounded theory. The evaluation index system was constructed from four aspects of township economic construction, township party building , township cultural confidence and township ecological civilization.The expert evaluation method was used to endow each evaluation index with five subordinate degrees, and the fuzzy analytic hierarchy process （FAHP） was used to determine the weight of each evaluation index. The fuzzy comprehensive inheritance degree evaluation model and the maximum membership principle were used to obtain the comprehensive inheritance evaluation grade of red culture in Beikuanping town, which was the maximum value of 0.347 in the comprehensive evaluation, and the evaluation result was corresponding to good ,and make the specific analysis in order to provide the basis for Shangluo rural red culture inheritance.

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Research on the dilemma and path of rural ecology civilization construction under the rural revitalization strategy: Based on the theoretical perspective of ecological modernization

LI Ye-zi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 203-205. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.046

Abstract （404）

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Ecological civilization was one of the five requirements of the rural revitalization strategy. Under the influence of the modernization process, the construction of rural ecological civilization was faced with the practical problems of lagging ecological governance concept, single ecological governance subject and limited ecological governance technology. From the theoretical perspective of ecological modernization, this research put forward optimization strategies from three aspects of ecological governance concept, ecological governance subject and ecological governance technology, so as to promote the construction of rural ecology civilization.

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Investigation and consideration on prosperity of industry in Jianghan Plain under background of rural revitalization： Take six villages in Jingzhou and Jingmen of Hubei province as examples

BIE Xiao-shuang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 206-210. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.047

Abstract （377）

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Industrial prosperity is an important basis for rural revitalization. It is an important task to highlight the advantages and characteristics, cultivate and expand rural industries, and accelerate the integration of primary, secondary and tertiary industries in rural areas. Jianghan Plain is an important part of the demonstration area of rural revitalization in Hubei province. Based on the investigation of six villages in Jingzhou and Jingmen of Hubei province, this study analyzes the current situation of rural leading industry development, summarizes the experience of rural industry development, and puts forward relevant suggestions for the problems found.

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Evaluation on county development level under the background of characteristic towns cultivation：Take Shandong province as an example

LIU Xuan, ZHANG Miao, LI Yan-ni, JIA Ji-ming, WANG Jie

HUBEI AGRICULTURAL SCIENCES 2020, 59 (16): 211-216. DOI: 10.14088/j.cnki.issn0439-8114.2020.16.048

Abstract （330）

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As an important part of the new urbanization and rural revitalization strategy,the cultivation of characteristic towns must be based on the development level and resources endowment of the county. The comprehensive evaluation index system of county development level was constructed from three dimensions of economic development, social service and ecological environment. The development level of counties （cities and districts） of 21 national characteristic towns in Shandong province was quantitatively measured and objectively classified by using entropy method and cluster analysis method. The results showed that,the development of 21 counties （cities and districts） exist problems of dual imbalances. One was the unbalanced development among the eastern areas,central Yimeng areas and inland remote areas; the other was the unbalanced development among economic development,social service and ecological environment. In order to promote the coordinated development of counties and improve the planning construction of characteristic towns, it was necessary to scientifically plan the layout of county competitiveness,optimize the resources allocation of characteristic towns; deeply excavate the industrial characteristic, condense the core competitiveness of characteristic towns,and strengthen the innovation carriers status of characteristic towns under the promotion of concept and technological innovation.

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Research status and prospect of rice planting mechanization

ZHANG Ni, ZHANG Guo-zhong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 5-6. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.001

Abstract （533）

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The research status and development trend of rice mechanized direct seeding and mechanized transplanting technology and equipment at home and abroad were summarized. According to China's rice planting conditions and planting system, the shortcomings of existing rice planting equipment were pointed out; rice transplanting was an important development direction of rice planting mechanization, and the research status of rice pot seedling transplanting was emphatically elaborated. Finally, the research direction and trend of rice planting mechanization technology were prospected.

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Research progress on the impact of urbanization and urban green space on urban birds

HE Meng, YANG Miao-chen, PEI Ying-feng, YANG Yuan-jiao, LUO Xu, WANG Yu-xiao, MA Chang-le

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 11-15. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.002

Abstract （501）

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Through reading the literature of “urban birds” from 2000 to 2019, three hot topics of urban ornithology research were summarized, namely, the impact of human interference on urban birds, the research on the relationship between urban green space, urbanization and urban bird community, and the impact of plant community composition on urban bird habitat. On this basis, the research status of urbanization and the impact of urban green space on urban birds was analyzed, and then the prospect of urban green space landscape design was put forward.

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Comparison of the effect of several drought-resistant agents on drought resistance and loss reduction in rice production

HOU Lin, LUO Yan-ping, HOU Xiao-jing, LUO Dong-yu, WEI Qiao, LI Hong-ju, ZHOU Jia-hua, JIN De-ming, WANG Fu, WANG Xiong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 16-18. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.003

Abstract （432）

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In order to find suitable drought-resistant agents for rice（Oryza sativa L.） production, field experiments were conducted using three candidate drought-resistant agents, namely, FA Handilong, Hanliting, and abscisic acid. The results showed that in the case of drought, all the three drought resistant agents could increase the chlorophyll content of leaves and panicle length of rice. In addition, FA Handilong could increase the seed setting rate, and Hanliting could increase the number of grains per panicle. Both Hanliting and FA Handilong could significantly increase the grain yield（P<0.05）. Rational application of these two drought-resistant agents in rice production may effectively relieve drought losses.

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Correlation between SPAD Value and nitrogen indicators in rice leaves at different growth stages

CHEN Qiu-yu, HUANG Ying-hua, ZHANG Hua-jie, ZHOU Li-yan, ZHAO Li-jun, ZHANG Shan-xuan, XIA Li-sha, HONG Rui-xia, LI Yan-da, CHEN Qing-chun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 19-24. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.004

Abstract （527）

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In order to clarify the correlation between the average SPAD value of rice （Oryza sativa L. ） leaves at different growth stages and the nitrogen content, accumulation of aboveground parts under condition of different amount of nitrogen fertilizer, a randomized block cultivation experiment under different nitrogen fertilizer levels was conducted. The results showed that the change trend of SPAD values measured in the upper, middle and lower parts of rice leaves was similar to the average SPAD values, and the SPAD values gradually decreased from tillering stage to yellow maturity stage. During the rice growth cycle, the nitrogen content of rice leaves and stems decreased gradually from tillering stage to yellow maturity stage, and the difference of nitrogen content increased with the increase of nitrogen application. During the whole growth period of rice, the average SPAD value of leaves was positively correlated with nitrogen content of stems and leaves（P < 0.01）. Therefore, the SPAD value of rice leaves can be used as a basis for regulating nitrogen application.

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Analysis of some quality characters of 14 wheat new lines

CUI Wen-li, CHEN Rui-rui, WANG Hui, ZHENG Wen-yin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 25-27. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.005

Abstract （341）

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In order to summarize the general situation of some new wheat lines, and to provide reference for the production of high-quality special wheat and the extraction of materials with excellent quality traits, 14 wheat breeding high-generation stable lines were selected, and some quality traits were determined by near-infrared analyzer. The results showed that the sedimentation values of 14 wheat strains were all low, but some strains reached the standard of high quality strong gluten wheat, with higher bulk density, higher protein content and higher wet gluten content. By comparison, the selection of C1, C2 and C9 lines can be strengthened.

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Investigation on present production situation and suggestions on structural adjustment of summer maize in Xiangyang city

LUO Zhen, TANG Hao-jun, ZHAO Yao, TAO Meng, LUO Zhen-mei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 28-30. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.006

Abstract （348）

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The present situation of summer maize （Zea mays L.） production in Xiangyang city was expounded from three aspectsvariety application, climate influence and processing application. The problems in planting structure adjustment of summer maize in the Xiangyang city were analyzed in combination with investigation, and the structural adjustment suggestions of “one stable and multiple adjustment” was put forward , that was, to develop disversified mode and improve planting level,and to steadily promote planting structure adjustment for production reference.

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Climatic characteristics and circulation type of snowstorm in the northern Tibet in the past 31 years

YU Yan-qun, XIONG Qiu-fen, Zuoma, De Ji Baizhen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 31-37. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.007

Abstract （386）

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Using the daily precipitation data, weather phenomena, and snow accumulation data of observatories in northern Tibet from 1988 to 2018, the climatic characteristics of snowstorms in the northern Tibet were analyzed by methods of meteorological diagnosis and wavelet analysis. The results showed that the spatial distribution of the number of snowstorm days, snow amount, maximum snow depth and annually average snow depth in the northern Tibet in the past 31 years generally showed a trend of more in the east than in the west, mainly concentrated in the east, with a significant regional difference. The number of the snowstorm and the amount of snow in the last 31 years showed a decreasing trend. The turning year of which was 2002, it was a snowy period before 2002, and it was a period of few snowfalls after 2002. After the 2000s, the number of snowstorms decreased at a rate of 2 stops every 10 years, and the amount of snowstorms decreased at a rate of 2.4 mm per 10 years. The season distribution of the snowstorm was bimodal, with the largest peaks occurring in April and May. The number of snowstorm days showed a periodic change of 3a, 5a, and 10a. Using NCEP to analyze the height field and wind field of the data, the circulation and impact system of snowstorm weather were summarized and concluded. The circulation pattern of the regional snowstorm in north Tibet could be divided into 4 types, namely, the Indian low-pressure type, the north ridge & south trough type, stepped trough type, and Balkhash lake low trough type. The southern branch trough, tropical depression, low vortex-shear and plateau trough were the main influence systems of the snowstorm in the northern Tibet.

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Study on the response relationship between the area change and climate of Dongting lake based on a long time series

WANG Wei, SUI Bin, LIN Nan, CHEN Tai-long

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 38-42. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.008

Abstract （380）

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Dongting lake is the largest reserve lake in the middle and lower reaches of the Yangtze river. It plays an important role in regulating the surrounding climate of the lake, and the climate also reacts on the lake. In order to study the response mechanism between the area change of Dongting lake and climate change, the long sequence data of water area of Dongting lake from 1989 to 2018 was used to analyze the spatio-temporal evolution characteristics of water area of Dongting lake, and the relationship between the change of water area and climate factors was studied. The results showed that the area of Dongting lake fluctuated significantly, with the largest monthly average area in July and the smallest in January. In recent 30 years, the water area of Dongting lake was in a general shrinking trend, and the annual maximum lake area and area change had a decreasing trend. According to the analysis of the lake area of nearly 30a with temperature and precipitation, the change of the water area of Dongting lake had a significant correlation with the surrounding climate conditions in the long-term evolution process, but the correlation was not significant in the short term. The change of lake area caused the temperature around the lake area to rise faster than the average rise rate of Hunan province and even the whole country.

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Effects of exogenous Se on the content of Cd and Se in rice grain under Cd pollution

WU Juan, GUO Zhen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 43-45. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.009

Abstract （327）

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Exogenous Se fertilizer was applied during the growth of rice to explore the accumulation effect of exogenous Se on Cd in rice grain under heavy metal Cd pollution. The results showed that in the Cd pollution environment, exogenous Se could decrease the accumulation of Cd in rice grains.

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Study on fertilizer utilization ratio of cherry tomato under limited root cultivation with coconut-coir substrate

TAN Hai-yan, ZHANG Zhen-rong, LIN Feng-ying, ZHANG Shao-ming, CHI Jing-di, TONG Jiang-yun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 46-48. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.010

Abstract （429）

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In order to implement substrates cultivation under root system control and improve the effect of fertilizer application, two different nutrient solution treatments were set up to study the utilization rate of nitrogen, phosphorus and potassium under coconut-coir cultivation of cherry tomato （Lycopersicon esculentum var. cerasiforme Alef.）. The results showed that the utilization rate of nitrogen, phosphorus and potassium of two treatments were 58%,44%,64% and 59%,30%,67%, respectively. After the cultivation, the pH of coconut-coir substrate was decreased, the partial nutrients and the salt content were accumulated.

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Effect of different combination of organic and inorganic potassium on the yield,quality and soil regime of lettuce

WANG Su-ping, DU Lei, HONG Juan, HUANG Xiang, ZHANG Gui-you, ZHANG Li-hong, LIAN Zhi-cheng, YE Li-xia, JIANG Li, CHEN Gang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 49-53. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.011

Abstract （392）

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In order to clarify the optimum proportion of organic and inorganic potassium fertilizer on lettuce （Lactucas sativa L. var. ramosa Hort.）, the effects of different combination of organic and inorganic potassium on the yield, quality of lettuce, the content of soil microorganisms and soil enzyme activity in Wuhan were studied. The results showed that the application of potassium fertilizer promoted the increase of leaves, SPAD value, photosynthetic rate and transpiration rate, decreased the content of nitrate and increased soluble sugar in the leaves, and significantly increased the yield, potassium content and potassium accumulation of lettuce, and also increased the activities of urease, phosphatase and invertase in the rhizosphere of lettuce after harvest, and the number of bacteria and fungi that could be cultured in the soil. Under the condition of equal potassium application, the application of organic fertilizer could reduce the accumulation of nitrate in leaves, reduce the content of VC in leaves and increase the content of soluble sugar. The yield of lettuce, accumulation of potassium, apparent utilization rate and agronomic efficiency of potassium fertilizer with the ratio of organic and inorganic potassium of 3∶7 and 1∶1 were all higher than other potassium treatments, and the activity of urease and phosphatase in the soil with the ratio of 1∶1 was also higher.Analysis indicated that the application of organic and inorganic potash fertilizer could improve the yield, quality, fertilizer utilization rate and soil enzyme activity of lettuce, and the effect of organic and inorganic potash fertilizer was better than that of all chemical fertilizers. The ratio of 3∶7 or 1∶1 of organic and inorganic potash fertilizer had better effect.

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Analysis and ecological risk assessment on heavy metal contents of farmland soils in Jiangxia district, Wuhan city

ZHANG Gui-you, WANG Su-ping, DU Lei, HUANG Xiang, JIANG Li, HONG Juan, LIAN Zhi-cheng, ZHANG Li-hong, YE Li-xia, CHEN Gang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 54-57. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.012

Abstract （427）

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A total of 149 soil samples were collected from farmland soils in Jiangxia district, Wuhan city. The contents of 8 heavy metals （Pb, Cd, Cr, Hg, As, Ni, Cu and Zn） were analyzed. The single factor pollution index and Nemerow synthetical pollution index were used to evaluate the heavy metals in farmland soils in this area based on the soil environmental quality risk management and control standard. The results showed that more than half of the farmland soils in Jiangxia distract were clean. The main pollution was Cd pollution, 40.27% of farmland soils were in the state of Cd pollution, about 1% of farmland soils were in the state of As, Ni and Zn pollution respectively, and no farmland soils were found to be contaminated by Cr, Cu, Hg and Pb.

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Rhizosphere soil microorganisms diversity of Cardamine violifolia O. E Schulz analyzed by Illumina MiSeq high-throughput sequencing system

CHEN Fei-fei, CONG Xing, XIANG Ji-qian, LI Hong-ying, SUN Ju-zhi, XU Feng, XUE Hua

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 58-62. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.013

Abstract （359）

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Taking nine soil samples growing Cardamine violifolia O. E Schulz from three area （Shuanghe town, Enshi city; Wufeng county, Yichang city, Hubei province and Shimen county, Changde city, Hunan province） as materials, the microbial community structure and diversity were analyzed by using Illumina MiSeq high-throughput sequencing system. The results showed that the microbial community composition of S1 soil sample form Shuanghe and S2 soil sample from Wufeng were similar, but the composition were quite different between S1 soil sample and S3 soil sample from Shimen, and were different between S2 and S3 soil sample. The diversity index and rarefaction curves analysis showed that in nine soil samples, the highest microbial diversity was No. S1.2 soil sample from Shuanghe and the lowest microbial diversity was No. S3.2 soil sample from Shimen. It indicated that the community diversity of Shuanghe soil sample was the highest and that of Shimen soil sample was the lowest. Proteobacteria was the dominant population in three area soil. The same phyla of the three area were Crenarchaeota, Acidobacteria, Actinobacteria, Bacteroidetes, Chlamydiae, Chloroflexi, Firmicutes, Gemmatimonadetes, Nitrospirae, OD1, Planctomycetes, Proteobacteria, Verrucomicrobia and WS3.

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Investigation and evaluation of ectomycorrhizal fungal resources in Xiangxidong forest park

LI Na, DU Hui-juan, HE Fei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 63-66. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.014

Abstract （522）

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The Xiangxidong forest park is a region with special natural geographical conditions and excellent ecological environment, located in Ankang city, Shaanxi province. Yet, little information is available on ectomycorrhizal fungal （ECMF） resources associated with various trees in this region. In this study, the ECMF resources in Xiangxidong forest park were investigated by the method of field survey, specimen collection and laboratory identification. In total, 16 ECMF species of eight families were identified, belonging to 8 families, including 4 species of Boletaceae, 3 species of Cortinariaceae, 2 species of Russulaceae, 2 species of Tricholomataceae, 2 species of Cantharellaceae, 1 species of Strobilomycetaceae, 1 species of Lycoperdaceae and 1 species of Sclerodermataceae. Among them, ECMF was most widely distributed in coniferous and broad-leaved mixed forests, accounting for 75.00%, 37.50% of fungi were found in coniferous forests, and 62.50% of fungi were found in broad-leaved forest. In the view of economic value, the fungi included 9 species of edible fungi（Excl. those who have been poisoned and those who have been safe to eat it）, 9 species of medicinal or anticarcinogenic fungi（including fungi that have medicinal and anti-cancer properties）, and 7 species of poisonous fungi（including fungi that toxic to them）. The ECMF resources in the Xiangxidong forest park are relatively abundant and have a good prospect for development and utilization.

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Study on the control effect of different pesticides on Empoasca （Matsumurasca） onukii

LIU Chao, PU De-qiang, HUANG Qiong, MAO Jian-hui, CHEN Yu, XIAO Ke-jun, LUO Xi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 67-68. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.015

Abstract （290）

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The field efficacy of three new agents of azadirachtin, eucalyptol and chlorothiazide on Empoasca （Matsumurasca） onukii was studied. The results showed that the three agents had certain control effects on E. onukii. Among them, 0.3% emulsifiable azadirachtin 250 times liquid had lower control effect; 5% solution eucalyptus oil 300 times liquid had better control effect, the control effect on the 3th day after treatment was 66.92%, and the control effect on the 7th day after treatment was 57.72%. The 10% soluble powder chlorothiazolate 750 times solution had good control effect. During the test, each treatment did not have an adverse effect on the growth of tea trees, and it was safe and harmless. Azadirachtin and eucalyptol are biological pesticide, and are environmentally friendly and could be used interchangeably with other chemical agents to delay the development of drug resistance.

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Effects of food factors on the period and survival rate of the first and second stage larvae of Holotrichia parallela

LIU Yan-tao, XI Guo-cheng, HU Tie-huan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 69-71. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.016

Abstract （360）

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Based on the indoor rearing methods of adult and larvae， the effects of different foods on the survival rate and the duration of the first and second instar larvae of Holotrichia parallela were determined. The results showed that the survival rates of the first and second instar larvae were significantly affected by different foods. The survival rates of the first and second instar larvae when they were fed with peanut roots were the highest， and it was lower when they were fed with cotton roots， peanut pieces and millet roots. When the first instar larvae was fed with potatoes， the development speed was fast and the concentration was high， while the larvae fed with cotton roots grew slowest and lasted the longest. The second instar larvae fed with potato， sweet potato and peanut roots developed faster， while the second instar larvae fed with cotton and millet roots developed slower and lasted longer. On the basis of this study， by adjusting the field layout and planting structure， the development duration of the larvae， the mortality rate， the population number and the harm to peanuts could be prolonged.

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Studies on the biological characteristics and phototaxis of the Hydrillodes repugnalis, a insect used for producing insect tea

FENG Shi-xin, WEI Yu-guo, WEI Yu-qian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 72-74. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.017

Abstract （425）

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The purpose of this study is to study the biological characteristics and phototaxis of Hydrillodes repugnalis, and increase yield of insect tea. The source of insects were obtained by attracting wild adults and bred by close net cages, then the development process and life habits of adults, larvae and pupae was observed regularly. While, the effects of monochromatic light waves and frequency conversion light waves on the photochemotactic behavior of adult worms were studied by photoethological method. The results show that in the production area of Guangxi, there are 3 generations per year. Pupation is in mid-March and emergence is in late April in the next year. Sexual maturation and mating occurs 2~3 days after emergence, and oviposition occurs 5~7 days after emergence. The growth course of each generation is different. The first generation is about 64 days, the second is about 72 days, and the third generation is about 205 days. Larvae growth period is the longest and adult period is the shortest in all generations. Adults are lurking in shallow food gaps or dark places during the day, act from eight to nine o’clock of night, and mating at night. Most eggs lay on the leaf surface of food layer, and are scattered. The larvae are photophobic and pseudo-lethal. The larvae are active in a warm, humid environment, and are fed on uncaria, jujube and fragrant leaves. In the range of 340 to 420 nm, the adult’s tendency to light is high. Gender and wavelength have certain influence on the chemotactic rate. The female adults have high phototaxis rateat wavelength of 360 nm, 390 nm and 350 to 369 nm, and the male adults have high phototaxis rate at wavelength of 360 nm, 420 nm and 350 to 369 nm. The adults of Hydrillodes repugnalis have strong phototaxis and concentrate in the range of 350 to 369 nm.

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Effect of temperature and matrix on seed germination of Lupinus micranthus

XU Lai-xian, AI Xun-ru, ZHANG Ying-tuan, ZHU Jiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 75-79. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.018

Abstract （332）

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By measuring the germination rate, germination energy, growth and seedling growth process of Lupinus micranthus seeds under different temperature and matrix conditions, the effects of temperature and matrix on the germination of L. micranthus seeds were analyzed. The results showed that, temperature had a significant effect on seed germination and seedling growth, and was the most important environmental factor for L. Micranthus seed germination and seedling growth. 15 ℃ was the most conducive to obtain a higher germination rate and 25 ℃ was the most conducive to obtain high growth. The matrix had a very significant effect on the maximum growth of L. micranthus, and had a significant effect on the initial stage and germination energy, but had no significant effect on the germination rate, end stage, germination days and average daily growth. The most comprehensive advantage of the matrix was perlite, vermiculite was the most conducive to the growth of seedlings. Temperature and matrix had obvious interaction effects on germination rate, germination period index, germination energy and maximum growth, the optimal treatment of temperature and matrix was “15 ℃+local yellow heart soil” and “15 ℃+peat”.

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Effect of temperature on growth and development of Petunia hybrida

BAI Yan-rong, WANG Jin-ying, JIANG Ya-lian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 80-82. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.019

Abstract （447）

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Taking Petunia hybrida double waterfall, single dream and single carpet as materials, the effects of temperature on the growth and development of P. hybrida were studied. The results showed that the growth and development of P. hybrida were influenced by temperature, the optimum temperature was 16~25 ℃, the plant height, crown width, lateral branches and the number of flowers increased the most at the constant temperature of 21~25 ℃, while the plants grew spindly and the number of flowers decreased when the temperature was 25~35 ℃. Temperature higher than 35 ℃ or lower than 15 ℃ inhibited the growth of P. hybrida, reduced disease resistance, large area of leaves wilting, or even dead.

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Effects of different shading treatments on flowering period and color quality of peony

YUE Gao-feng, HAN Zhi-qiang, MA Jun-qiang, XU Xian-xin, YANG Wen-yue

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 83-86. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.020

Abstract （432）

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Paeonia suffruticosa Andr var. papaveracea （Andr.） Kerner was used to study the effects of different shading treatments on the flowering period and color quality of P. suffruticosa. The results showed that the light conditions under different shading degrees had obvious effects on the flowering period and color quality of peony. The full flowering period under 40% and 60% shading was 3 and 6 days later than that under full sunlight and the number, diameter, fresh weight and flower rate of peony were the best. 20% shade significantly improved the ornamental value of red peony; Under 40% shading, except for purple, the ornamental value of white, pink, red and black lines decreased; The effect of 60% shading on the flower color of all tested color systems was very obvious, which seriously affected and reduced the ornamental value of flower color.

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Comparison of drought resistance of five kinds of Hainan winter crops

YAN Shi-long, QIAN Kun, MENG Rui, AO Su

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 87-92. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.021

Abstract （527）

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In this experiment, polyethylene glycol（PEG-6000） was used to simulate drought conditions, the effects of PEG-6000 on five species including Lycopersicon esculentum, Zea mays L., Brassica chinensis, Citrullus lanatus and Raphanus sativus seed germination and seedling growth were investigated. The results showed that the germination rate, germination potential, germination index, germination rate and average germination time of the five tested plants were obviously affected under high concentration PE-6000 treatment. The comprehensive evaluation of the subordinate function values showed that the order of drought resistance of the five tested plants was as followsZea mays L. > Brassica chinensis > Lycopersicon esculentum > Citrullus lanatus > Raphanus sativus.

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Effects of pioglitazone on oocytes maturation in vitro and parthenogenetic embryos development of pig

LYU Ling-yan, WANG Yan-ling, PAN Xing-chen, ZHANG Jia-qing, WU Yong-shao, SUN Ru-yu, PAN Tian-biao, ZHANG Bing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 93-96. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.022

Abstract （243）

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Aiming to investigate the effects of pioglitazone（PIO） on oocytesmaturation in vitro and parthenogenetic embryos development of pig, the effect of different concentration PIO on oocytes maturation in vitro and parthenogenetic embryos developmental potency were compared. The results showed that the polar body excretion rate of 1.0 μmol/L PIO-treated group was significantly higher than that of 0.5 μmol/L PIO-treated group in maturation medium（P<0.05）,was very significantly higher that of control an 1.5 μmol/L PIO-treated group（P<0.01）. The mature oocytes cultured in different concentrations PIO were parthenogenetic activated, the experimental results showed that the cleavage rate of the 1.0 μmol/L PIO group was significantly higher than of the 0.5 μmol/L group in embryo culture medium（P<0.05）, was very significantly higher than that of the control and 1.5 μmol/L PIO group （P<0.01）, as for the blastocyst rate and the total number of cells, the group of the 1.0 μmol/L PIO-treated group was the highest among the groups （P<0.01）. In conclusion, 1.0 μmol/L PIO in maturation medium could improve the maturation rate of porcine oocyte, also could significantly improve the blastocyst rate and the total number of cells of porcine parthenogenetic embryos.

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Study on phages in control of common bacterial diseases in Macrobrachium nipponense and its application

HANG Xiao-ying, YU Zhe, LYU Sun-jian, SHI Wei-da, YUAN Xue-mei, LIU Li

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 97-100. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.023

Abstract （426）

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41 bacterial strains were isolated from water and shrimps of three Macrobrachium nipponense ponds, by 16S rDNA gene sequence analysis and colony morphology observation, the strains from water were preliminarily identified as Bacillus megaterium, Aeromonas caviae, Enterobacter roggenkampii,et al, the strains from shrimp were preliminarily identified as Aeromonas caviae, Citrobacter murliniae, Enterobacter roggenkampii, et al. The phage NTHP01 inhibition of bacteria was detected by drop injection methodthe results showed that, phage NTHP01 had good cracking effect on Bacillus, Aeromonas, Acinetobacter, Enterobacter, Citrobacter, Arthrobacter protophormiae, Orthomonad, Lactococcus garvieae, et al. Phage NTHP01 preparation was appliled in shrimp breeding pond, the preliminary results showed that, the yield of Macrobrachium nipponense increased by 338~412 kg/hm², and the output value increased by 2.25 ten thousand ~3.00 ten thousand yuan/hm².

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Study on the technique of pond cage culture of Siniperca chuatsi with compound feed

LI Sheng-hua, HUANG Yong-tao, WANG Cong-dan, WU Fan, XIA Chen, ZHANG Cong-yi, HOU Hai-ying, CHEN Xia, XU Wei, ZHANG Liang-ming, SHI Yi-yuan, XIAO Zhi-hua, LI Xu-chun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 101-106. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.024

Abstract （367）

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In order to to study the technique of Siniperca chuatsi with compound feed, the artificial ecology was set up in three ponds, and the net cages were less than 10% of the total area of the pond. Fishes with different niche and feeding habits were controlled outside the cages to control the total biomass of the ponds; Siniperca chuatsi seedlings were placed in the cages. Feed a compound feed combined with a bait made with Chinese herbal medicine ingredients. The result showed that, no disease occurred during the breeding period. The water quality of the three test ponds was mild eutrophication for 52.4% of the time and moderate eutrophication for 47.6% of the time. The yield of commercial Siniperca chuatsi was 15 to 18 kg/m², and the average pond production was 12 700 to 14 400 kg/hm², breeding profit 194 400~249 900 yuan/hm², commercial Siniperca chuatsi Besilewsky specifications were neat, catching and selling were convenient, and economic and ecological benefits were significant.

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Study on extraction technology and content of coumarin from three varieties of Viburnum

NIU Yin-yin, LIAO Teng-fei, WANG Li, SUN Min, XU Ping, LUO Xing-wu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 107-111. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.025

Abstract （342）

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The raw materials are Viburnum rhytidophyllum,Viburnum propinquum and Viburnum sargentii. The single factor experiment was carried out by selecting ethanol concentration, extraction time, extraction temperature and ratio of material to liquid as factors, absorbance as index, and then orthogonal test analysis was carried out at appropriate factor level and HPLC was used to carry out the experiment. The yield of coumarin from three varieties of Viburnum was compared by the optimized extraction process. The results showed that coumarin was found in all three kinds of Viburnum, Viburnum rhytidophyllum was the highest,Viburnum propinquum was the second, and Viburnum sargentii was the least. The optimum conditions of Viburnum rhytidophyllum and Viburnum propinquum were as followsThe concentration of ethanol was 55%, the extraction time was 60 min, the extraction temperature was 80 ℃, and the ratio of material to liquid was 1∶25. The optimum conditions of Viburnum sargentii were as followsThe concentration of ethanol was 55%, the extraction time was 80 min, the extraction temperature was 70 ℃, and the ratio of material to liquid was 1∶25. The content of coumarin was different in the extraction process of different varieties of Viburnum.

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Optimization of extraction technique of polyphenols from Hemsleya chinensis by response surface methodology

ZHOU Zi-shun, DUAN Ke-zhao, QUAN Rui-lan, ZHANG Guang-hui, YANG Sheng-chao, ZHAO Yan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 112-118. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.026

Abstract （342）

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Taking Hemsleya chinensis as the experimental material, the Box-Behnken central composite design and response surface methodology were used to optimize the extraction conditions of polyphenols from Hemsleya chinensis. The extraction rate of polyphenols was used as index. Based on the results single factor experiments, a mathematical model of 4 factors and 3 levels center combinations was established with the Design-Expert 8.0.6 software on the extraction temperature, ethanol concentration, liquid to material ratio, extraction time. The results showed that the fitting degree of polyphenol extraction rate model was good and significant. The best process parameters were extraction time 59 min, ethanol concentration （V/V） 50 %, extraction temperature 74 ℃, and liquid to material ratio 16∶1（mL/g）. Under these conditions, the extraction rate of polyphenols from H. chinensis was （1.479 6±0.004 1） mg/g.

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The low temperature extraction method of cellulose from corn straw

SONG He-ming, JIA Hong-ge, HU Yu-jie, CHENG Hong-bo, ZHAO Xin-yi, WANG Qing-yu, XU Jing-yu, WANG Xue-feng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 119-121. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.027

Abstract （427）

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In this study, a new method of extracting cellulose from corn stalk at low temperature was explored. Cellulose was successfully extracted from corn straw by low temperature system. The cellulose samples were characterized by FTIR, TGA and XRD. The results showed that low temperature system was an environmental protection system, which could extract high purity cellulose from corn straw.

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Fuzzy mathematics comprehensive evaluation method for optimizing the formula of Hovenia dulcis beverage

ZHU Xin-peng, REN Xing-xing, YUAN Zhi-ning, WEI Mei-juan, MING Jing, CHENG Zhu-lin, WANG Jin-meng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 122-127. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.028

Abstract （373）

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Using Hovenia dulcis concentrated juice as raw material through orthogonal test and based on single factor test, taking fuzzy mathematics comprehensive evaluation as index to optimize the formula. The results showed that,100 mL Hovenia dulcis beverage needed 20 mL Hovenia dulcis concentrated juice, the ratio of sucrose to acesulfame was 5.000 g∶0.020 g, the dosage of citric acid was 0.20 g, the ratio of lemon yellow to carmine was 0.100 mL∶0.050 mL, the ratio of xanthan gum to guar gum was 0.02g∶0.02g. The formula makes Hovenia dulcis beverage in harmony with sweet and sour, having delicate fragrance of Hovenia dulcis. It can be used in developing of Hovenia dulcis beverage.

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Preparation of calcium peroxide from kitchen waste egg shell and its application in fruit preservation

YAN Zong-lan, YANG Fu-sheng, ZHAO Peng-ying, PAN Hong, BU Lu-xia

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 128-131. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.029

Abstract （406）

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The eggshell of kitchen waste was pretreated to study the content of calcium peroxide produced by the reaction of eggshell with and without film. It was found that the content of calcium peroxide in the sample without membrane was 54.28%, and that in the sample with film was 50.97%. The preservation of bananas and strawberries with calcium peroxide was studied. With the passage of time, it was found that the higher the amount of calcium peroxide was added, the lower the weight loss rate of fruits was. The weight loss rate of samples without calcium peroxide preservative was the highest. The results showed that calcium peroxide could inhibit the water loss of bananas and strawberries, prolong the fresh-keeping period, and had a strong fresh-keeping effect.

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Application of CMC-Na in coating preservation of Chaenomeles sinensis

XING Jie

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 132-136. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.030

Abstract （356）

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In this study, sodium CMC was used as coating material to investigate the effects of coating solution on the storage and preservation of Chaenomeles sinensis, including the time-dependent change of mass loss, VC content, organic acid content, total sugar content, flavonoids content and total polyphenols content. In order to obtain the optimal treatment concentration of CMC-Na and extend the shelf-life of Chaenomeles sinensis, to provide references for the industrialization of Chaenomeles sinensis. The results showed that CMC-Na film could effectively reduce the mass loss of Chaenomeles sinensis during storage, inhibit the decrease of organic acid, total sugar, flavonoids, total polyphenols and the degrandation of VC. Among all the treatments, the massfraction of 1.0% in CMC-Na had the best preservation effect, which could effectively prolong the fresh-keeping period of Chaenomeles sinensis.

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Study on fresh-keeping condition of cut rose flowers for household use

DENG Tian, HU Ting, YAN Li

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 137-141. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.031

Abstract （418）

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To study the fresh-keeping effects of sprite, salt, vinegar, beer, vitamin C, detergent and citric acid on fresh cut rose flowers. The best fresh-keeping reagent, the best concentration, the best pretreatment temperature and the best length of cut rose were determined by single factor experiment. The best preservation combination was optimized by L₉（3⁴） orthogonal test with the best pretreatment temperature, the best concentration of reagent and the best length of flower branch as the factors and the changes of flower diameter, fresh weight and vase life as the indexes. The results showed that with the increase of vase time of cut rose flowers, the life of fresh-keeping liquid containing sprite was the longest, and it was 5 days longer than that of control group, and the time of reaching the maximum flower diameter was the longest, and the ornamental effect was the best. After low temperature pretreatment, the fresh-keeping effect of fresh-cut flowers was significantly improved, the life span of cut flowers was about 20 days. Different flower branch length had little effect on fresh cut flowers. The results showed that the best fresh-keeping reagent of cut rose was sprite, the best concentration was 2%, the best low temperature pretreatment was 3 ℃, the best length of flower was 30 cm. The optimum preservation conditions were low temperature pretreatment at 3 ℃, reagent（sprite） concentration at 2%, flower diameter length at 25 cm.

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Determination of 13 pesticide residues in Wuniuzao tea by SPE-GC-MS/MS

LEI Hai-yan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 142-144. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.032

Abstract （448）

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To simultaneously determine 13 pesticides in 50 samples of local tea Wuniuzao. The samples extracted by acetonitrile and dried by rotated, purified by Cleanert TPT column,then subjected to GC-MS/MS using internal standard method. The treated samples pesticide residues were not detected. The results showed that the Wuniuzao of the local mountain area were very low. This paper provides some basic theory for the development of local tea industry.

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Detection of biochemical components and quality analysis of ancient tea tree black tea of zhuquesi in Ziyang population species

LUO Ting-ting, ZHANG Lin, HAN Hao-yu, CHEN Fang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 145-149. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.033

Abstract （431）

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The quality characteristics of the spring and autumn rosefinch black tea samples in 2018, were judged by compared with the main content of biochemical components and sensory quality evaluation. The results of sensory evaluation showed that spring rosefinch black tea was brown and bright, uniform and tight, sweet and lasting, and the soup color of red and bright. Autumn rosefinch black tea was slightly loose in shape, but still had the characteristics of sweet and mellow, and the soup color was bright and so on. The comprehensive scores showed that spring black tea belonged to the first grade second class tea, and autumn black tea belonged to the third grade first class high quality tea. The results of physical and chemical examination showed that the total ash and water extract content of spring and autumn black tea samples were in accordance with the national standard. The contents of tea polyphenols, caffeine, free amino acids and tea pigment were little different from those of black tea from other producing areas in China. The average content of trace element selenium is in the range of 0.038 1~0.263 0 mg/kg. The contents of heavy metals Pb,As,Hg and Cd were lower than the China national export quality standards of tea. Moreover, the 48 pesticide residues were not detected in spring and autumn tea samples. Therefore, it can be seen that the spring and autumn rosefinch black tea samples are according with the relevant national standards, and the whole level of the black tea reaches the pure natural organic high-quality black tea, whether it is in the sensory aspect or on the main contents and pesticide residues.

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Improving detection of malachite green residue in aquatic products by HPLC

ZHU Zhi-qiang, TONG Wen-yu, ZENG Zhi, ZHANG Liang-ming, SHI Yi-fu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 150-153. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.034

Abstract （391）

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Malachite green, as a synthetic industrial dye of triphenylmethane, has been widely used in aquaculture as an insecticide and fungicide. GB/T20361—2006 for the detection of malachite green residues in aquatic products was found that had some disadvantages, such as tedious operation, poor recovery and precision during years of testing. The effects of different extraction times and purification times on the recovery rate, and improved the pretreatment and chromatographic conditions of GB/T20361—2006,reducing the number of extractions and purification operations reduces the flow rate of the chromatographic method. The improved method has a detection limit of 0.40 μg/kg, a recovery rate of 81.8%~100.8%, and a precision of 0.93%~8.32%, which all meet and exceed the national standard.

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HPLC analysis of sulforaphane in different kinds of leaf mustard （Brassica juncea Coss. var. foliosa Bailey） seeds

LAN Mei, YANG Hong-li, HU Jing-feng, ZHANG Li-qin, XU Xue-zhong, HE Jiang-ming

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 154-157. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.035

Abstract （436）

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In order to get the resources of leaf mustard with abundant sulforaphane,seeds of twenty cultivars were used to analyze and compare the contents of sulforaphaneby high performance liquid chromatography （HPLC）. The results showed that the range ofvariatoin of sulforaphane was 11.79~226.72 mg/kg（DW） with significant differences among tested lines. The material （Tai le sijiqing） with highest content of sulforaphane,which is 226.72 mg/kg （DW）,was about 19.2 times compared with the lowest one （Spring cobbage with round leaves） which is 11.79 mg/kg （DW）. It provides an foundational material for further research on rule of variation and the mechanism of metabolism of sulforaphan.

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Determination of chromium in food by ionic liquid-graphite furnace atomic absorption spectrometry

LING Yue-tao, PAN Rui-hua, WANG Yao-xue, WANG Fan, GUO Shao-fei, ZHANG Lin, ZHAO Li-yang, XING Jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 158-160. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.036

Abstract （302）

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A method for the determination of chromium in food by ionic liquid -graphite furnace atomic absorption spectrometry was established. The sample was directly dissolved in 1-butyl-3-methylimidazolium chloride （[Bmim]-Cl） and added with 0.5 mL nitric acid. The pretreatment process could be completed within 5 minutes. The solution was directly detected by graphite furnace atomic absorption spectrometry. Chromium has a good linear relationship in the range of 2.58~20.64 μg/L, under the optimized spectral conditions, the detection limit of chromium is 0.04 mg/kg, the recovery of standard addition is 92.00%~101.65%, RSD is 4.11%~6.69%. The pretreatment method is green and safe, easy to operate and fast to process, and has a wide application prospect.

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Design and experiment of the controller of shutter machine in solar greenhouse

CUI Yu-xiang, ZHAO Hui-fang, ZHAO Liang, YANG Fang, YANG Rui, PEI Wei-feng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 161-165. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.037

Abstract （356）

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The daily operation of curtain rolling machine is one of the main work of winter heat preservation in chinese solar greenhouse. A controller based on the minimum system of STM8S003F3 microcontroller was designed. With timer and EEPROM, the control program was designed according to the timing principle, and the semi-automatic operation of the curtain rolling machine was realized. Through wireless communication, the controller had remote control operation function. The controller has the advantages of low cost, simple principle and reliable operation, and reduces the labor burden.

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Design of automatic weather station based on Aliyun Internet of things platform

HUANG Jiao-yu, TANG Hai

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 166-169. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.038

Abstract （461）

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In view of the difficulty of maintenance and high professional requirement of meteorological station used by governmentalmeteorological department, a design scheme of civil automatic weather station based on Aliyun Internet of things platform is proposed. The system design includes hardware and software. The hardware part includes the core circuit of AM3358 controller, Modbus sensor data acquisition circuit ,4G communication module and so on. The software part includes device side Link kit software development, cloud server side IoT Studio website application UI interface development, background data service development, message flow and so on. All functions of the six-element automatic weather station are implementedin the system, and some practical functions are added, such as sending the message to the mobile phone APP group when a specific weather parameter appears. The practical test shows that it has the advantages of simple maintenance and low cost, which is especially suitable for the observation of meteorological elements in non-meteorological departments such as agricultural production, station and port.

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Method for preparing Escherichia coli electrotransformation competent cells by rapid desalting

CAI Song, YANG Dong-cheng, TANG Mei, XIONG Yuan-yuan, WANG Jin-hua, WANG Yong-ze

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 170-174. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.039

Abstract （348）

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A similar density gradient centrifuge method was adopted to establish an efficient and fast protocol for Escherichia coli electrotransformation. Angle rotor centrifuge was used to achieve the goal of rapid desalination of cells using 20% glycerol （V/V） /1.5% mannitol （m/V） solution as gradient medium. The ratio of bacteria suspension to gradient medium was 1∶5 （V/V） to form a density gradient, which led to effectively centrifugal desalination. After centrifugation the cells were resuspended in a mixture of 20% glycerol （V/V）/1.5% mannitol （m/V）, sequentially electroporated at 15 kV/cm. This procedure was suitable for effective desalination. Electroporation at room temperature did not improve transformation efficiency. In this study, the electransformation efficiency can reach 4.2×10⁹ CFU/μg DNA only by two centrifugation, which greatly simplifies the experimental procedure.

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Rapid identification of differential protein related to rice quality by iTRAQ

ZHA Wen-jun, YOU Ai-qing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 175-177. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.040

Abstract （324）

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Using Ezhong No. 5 and Guichao No. 2 as materials, the iTRAQ technology was used to quickly identify rice quality differential proteins, so that candidate genes related to rice quality can be rapidly and effectively screened based on the differential proteins. The results showed that Ezhong No. 5 and Guichao No. 2 identified a total of 61 significantly different proteins in rice, including 21 up-regulated proteins and 40 down-regulated proteins. This technology can be widely used in the research and development of rice quality traits and combined with transgenic technology to cultivate high-quality rice varieties.

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Research on the dilemma and solution of the integrated development of urban and rural economy in China

LI Ping-nyu, LIU Jun-xia

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 178-181. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.041

Abstract （470）

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Starting from the imbalance of urban and rural economic development in China, the main difficulties faced by China's urban-rural economic integration development were analyzed. The policy of urban bias has far-reaching impact, the level of agricultural modernization needs to be fully improvedand the rural environmental problems need to be fully rectified. The realization path of the integration of urban and rural economy in China was put forward.

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Opportunities and challenges for agriculture, rural areas and farmers under the impact of COVID-19

CAO Zhi-jie, YANG Xue

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 182-184. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.042

Abstract （504）

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The huge challenges and development opportunities of China's agriculture, rural areas and farmers were analyzed under the impact of COVID-19. How to promote the common development of agriculture, rural areas and farmers with the cooperation of government, enterprises, social organizations and individuals was discussed.

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Research on the cultivation of new agricultural management subjects based on economic transformation

WANG Ying

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 185-188. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.043

Abstract （278）

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Under the background of economic transformation, cultivating new agricultural management entities is not only an inevitable requirement for economic structure adjustment and upgrading, but also an inherent need for agricultural industrial structure adjustment and upgrading. Based on grasping the logic of the new agricultural management subject cultivation system, the problems existing in the cultivation of new agricultural management subjects in the process of transformation was analyzed. The empirical analysis on the factors influencing the cultivation of new-type agricultural business entities was made. The corresponding countermeasures including improving the rural land circulation market, innovating financing methods, building agricultural socialized service system, establishing risk prevention mechanism and strengthening agricultural vocational and technical training were put forward, so as to better cultivate new agricultural business entities and promote economic transformation and upgrading.

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Study on the distribution of income from market circulation of rural collective business construction land

BAI Shan-wen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 189-192. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.044

Abstract （338）

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At present, the entry of rural collective business construction land into the market has been approved by the Third Plenary Session of the 18th Central Committee of the Communist Party of China on“equal access to the market, same right and same price” between urban and rural unified construction land and state-owned land in 2013. At the same time, article 63 and article 34 of the “Land Management Law” also have relevant provisions on the entry of collective business construction land into the market. With the promotion of the reform of rural collective business construction land, there are more and more researches on the distribution of construction land transfer income. This study mainly analyzes the theoretical basis of rural collective business construction land, the risk of entering the market and the ideas and strategies of value-added income distribution in order to improve the reference value.

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Research on performance evaluation of rural sewage network governance based on BSC：Take Changyuan city,Henan province as an example

ZHU Li-juan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 193-198. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.045

Abstract （360）

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The management and protection of rural water environment involves multi-stakeholders. The joint participation of multiple subjects are the key to improve the effectiveness of governance. Based on the analysis of the current status of rural sewage network treatment in Changyuan city, Henan province, the balanced score card is used as a performance evaluation tool, this study sets and evaluates the performance of rural sewage network governance in Changyuan city of Henan province from four dimensions of finance, customers, internal operations, learning and growth. The results show that the endogenous network governance model with villages as the main body, government guidance, and social resources support is more effective, which will help to open a new situation of rural sewage treatment.

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Institutional economics analysis on the three rights division of rural housing land：A case study of Changyuan city in Henan province

XU Chun-lian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 199-203. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.046

Abstract （350）

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Taking “relative price change of land elements-benefit game-reform on the three rights division of rural housing land” as the main line, this study analyzes the institutional economics of the reform of the three rights division in Changyuan city, Henan province, by means of theoretical analysis and literature review. It is found that the change of relative price of land factors increases the external profit, which also becomes the practical demand of the reform of the separation of three rights division. The reform of the three rights division is conducive to the development of local economy, the improvement of farmers’ living environment, and the strength of collective economic organizations. It can also realize the internal internalization of external profits among farmers, governments and collective economic organizations through benefit game, so as to achieve a win-win situation. Therefore, we should respect the change trend of relative price of homestead elements, recognize the value attributes of homestead assets, construct a value-added revenue sharing mechanism agreed by all stakeholders, and manifest the institutional performance.

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Study on the change and efficiency of rural land resource utilization：A case on Anhui province

ZHU Yuan-yuan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 204-208. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.047

Abstract （363）

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Agricultural production efficiency determines the level of rural regional economic development and the quality of people's life. The mode and structure of rural land use in Anhui province are changing constantly. By constructing multi-factor evaluation index, the land use efficiency was analyzed comprehensively by using data envelopment analysis. The results showed that the comprehensive efficiency of rural land use in Anhui province showed an increasing trend, and there were significant differences among different regions. Therefore,to improve the utilization efficiency of rural land use, we should strengthen the service function of the government, perfect the infrastructure construction, perfect the supporting system of land circulation and enhance the value of land resources.

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Study on the adaptability of the Three Gorges trans provincial migrants in the post immigration period：Based on the sample survey of resettlement sites in Tongling city, Anhui province

NIE Qian-yu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 209-213. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.048

Abstract （325）

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The resettlement of the Three Gorges project is the largest project resettlement in China. By the end of 2009, nearly 200 000 rural migrants had been relocated in the Three Gorges Reservoir area. Since 2000, the first group of immigrants to cross the province had been living there for nearly 20 years. The social adaptation of the immigrants in Tongling city, Anhui province is related to the survival and development of the immigrants in the Three Gorges, and also has an important impact on the community management and economic development of the immigrations. This study analyzed the adaptability of the Three Gorges immigrants in Tongling city, Anhui province by mean of literature method, interview method and field observation method, and studied the social adaptability of immigrants from the aspects of economy, politics, culture and social environment. It was found that there were some problems such as unsatisfied compensation, poor housing quality, petition for rights protection and disturbance and so on. In the post immigration period, we need to pay attention to the problems of employment, prosperity and ecological environment construction of immigrants.

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Research on the supply of rural public services in county areas from the perspective of collaborative governance

XIANG Jia

HUBEI AGRICULTURAL SCIENCES 2020, 59 (17): 214-217. DOI: 10.14088/j.cnki.issn0439-8114.2020.17.049

Abstract （319）

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Based on the theory of collaborative governance, focusing on county rural areas, the basic elements of county rural public service supply, namely, the four collaborative subjects of farmers' demand, government, market and other multiple subjects of society were extracted. The collaborative structure was analyzed from the strategic level, structural level and operational level; the county rural public service supply system was expounded with the collaborative process of establishing trust relationship, reaching ideological consensus, effective participation and collaborative ability. On this basis, the optimization path of public service supply from the perspective of collaborative governance in terms of concept updating and strategy selection was put forward.

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Research progress on the effects of histone deacetylase inhibitors on in vitro developmental competence of nuclear transfer embryos

LYU Ling-yan, WU Yong-shao, ZHANG Jia-qing, SUN Ru-yu, WANG Yan-ling, PAN Tian-biao, CHEN Bao-jian, XIE Bing-kun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 5-8. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.001

Abstract （404）

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Histone deacetylase inhibitor （HDACi） is a class of compounds that can interfere with histone deacetylase to control the tightness of DNA binding to histones and play a role, which can increase the degree of histones acetylation in cells and improve the expression level of acetylated genes. In recent years, the researches on HDACi promoting epigenetic reprogramming of donor cells and nucleartransfer embryos and regulating the genes expression in higher animals have attracted much attention. The effects of HDACi on the in vitro developmental competence of nuclear transfer embryos are mainly described, in order to provide reference for improving the production efficiency of cloned embryos.

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Progress and trend of rural tourism research in the background of rural revitalization strategy： Based on the visual analysis of CiteSpace

REN Li-li, YAO Hong, XU Xiang-fa

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 9-14. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.002

Abstract （553）

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Rural tourism has become a recent research hotspot under the background of the national rural revitalization strategy. In order to clarify the characteristics of preliminary research of rural tourism and the development trends under the new strategy, this article uses the CiteSpace visualization analysis tool to draw scientific knowledge map of rural tourism research for more than two decades from 1992 to 2019, exploring the distribution of researchers in related fields, the distribution of research institutions, and the hotspots of rural tourism research. Furthermore, comparing the evolution of rural tourism research trends before and after the rural revitalization strategy was proposed, through the visualization of the keyword co-occurrence network atlas, to explore the hot topics and analyze the frontier research trends under the background of the rural revitalization strategy.

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Selection of selenium-enriched rice varieties

ZHOU Qiong, TIAN Yong-he

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 15-17. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.003

Abstract （419）

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Using selenium content as an index, the selenium content of rice samples grown in selenium-rich and low-selenium areas in Ankang were analyzed, the high-quality rice varieties with the best selenium accumulation capacity were selected, and the selenium accumulation capacity of different parts of rice was analyzed. The results showed that the variety Chuanyou 2115 and Hongmi 1 had better ability of selenium accumulation, and the protein content was positively correlated with the selenium content in rice. The distribution of selenium in different parts of rice was root>leaf>stem>rice.

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Demonstration of direct seeding rice with different seeding amounts and high-yield cultivation experiments in Senegal

ZENG Fan-cheng, WANG Li, YANG Yu-bo, ZENG Yun, GUO Xin-bo

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 18-20. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.004

Abstract （436）

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Senegal is rich in land and water, and is a high-yield region for rice production. However, the increase of local rice production is restricted by various factors. “South-South Cooperation” rice planting experts from China solved the important factors restricting the increase of rice yield by conducting demonstrations of direct seeding rice with different seeding amounts and high-yield cultivation experiments, which achieved significant results. It has solved the important factors restricting the increase of rice yield and achieved obvious results, and formed the high-yield cultivation technology achievements of “South South cooperation” direct seeding rice.

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Effects of exogenous 5-aminolevulinic acid on the germination and seedling growth of mung bean under NaCl stress

ZHAO Jin-xi, YOU Ying, JIANG Kai-yang, ZHANG Jia-xiang, ZHANG Mei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 21-24. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.005

Abstract （397）

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The effects of different concentrations of exogenous 5-aminolevulinic acid on seed germination and seedling growth ofmung bean under salt stress were studied by using mung bean as test material and 150 mg/L concentration NaCl as simulated salt stress. The results showed that different concentrations of 5-aminolevulinic acid promoted the germination and seedling growth of mung bean at low concentration and inhibited at high concentration under NaCl stress, the germination rate and germination potential of green beans increased first and then decreased with the increase of treatment concentration, reaching the highest concentration of 50 mg/L and 150 mg/L respectively. Exogenous 5-aminolevulinic acid played a significant inhibitory role when the concentration reached 250 mg/L. The leaf area and root length of mung bean at seedling stage increased first and then decreased with the increase of treatment concentration. When the treatment concentration reached 100 mg/L, both reached the maximum value. With the increase of 5-aminolevulinic acid concentration, malondialdehyde （MDA） decreased at first and then increased, and reached the lowest when the treatment concentration reached 150 mg/L. The content of proline and soluble sugar increased first and then decreased, and reached the highest when the treatment concentration reached 150 mg/L and 100 mg/L, respectively. The results showed that 5-aminolevulinic acid had a significant effect on the stress resistance of mung bean seedlings. The optimal concentration of exogenous 5-aminolevulinic acid on mung bean germination under NaCl stress was about 100 mg/L, and the optimal concentration for seedling growth of mung bean under NaCl stress was 150 mg/L.

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Analysis of cotton production situation in cotton areas of Hubei province and Yangtze river basin in the last five years

ZHANG Jiao-hai, YI Guo-xiang, BIE Shu, WANG Xiao-gang, XIA Song-bo

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 25-28. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.006

Abstract （350）

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The production situation of cotton in Hubei province and Yangtze river basin in recent five years was summarized and analyzed. The reasons for the change of area and production capacity were analyzed, and the achievements of cotton planting project and the progress of cotton mechanical harvesting were briefly described. The policy and technical measures to develop cotton production and improve the effective supply of cotton in the Yangtze river basin were puts forward.

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Study on the meteorological insurance index of high temperature and heat damage of rice in central of Hunan province

DENG Mei, WU Meng-qiong, ZHAO hui, WANG Xue-qi, CHEN Tie-jun, XIAO Shi-yao, XIAO Miao-ni

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 29-32. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.007

Abstract （403）

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A rice planting demonstration county in central of Hunan-Shuangfeng county was selected as a pilot, using daily weather observation data, rice yield and rice observation data of agricultural meteorological observatories from Shuangfeng county from 1989 to 2018, combined with the main types of agrometeorological disasters during the growth period of rice, the meteorological disasters with large impact on production were identified as insurance hazard factors to design the meteorological insurance index.The research showed that the high temperature heat damage was the main meteorological disaster that caused single cropping rice yield reduction in central of Hunan. High temperature heat damage meteorological insurance index was established based on the comprehensive hazard heat accumulation temperature and the number of continuous days; By calculating the rice production reduction and currency loss caused by meteorological disasters over the years, the compensation trigger coefficient and compensation standard of the high temperature heat damage meteorological insurance index were determined.

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Analysis and regionalization of agricultural climate resources in Cheng county

CHEN Jian-xiong, XIA Xuan, LIU Li, WU Xin-chang, LI Jia, SHE Lu-qing, GAO Dang-feng, ZANG Juan, HE Xiao-dong, WANG Xin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 33-38. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.008

Abstract （389）

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Statistical principles, spreadsheets and GIS techniques were used for statistical analysis of meteorological and agrometeorological observation data and hydrological data of Cheng county of Gansu province from 1981 to 2015. At the same time, the climate resources, climate change status, the degree of meteorological disaster and the influence of climate on planting and breeding in Cheng county of Gansu province were analyzed with the data from agriculture, forestry and breeding industry. With illustrated in the form of comprehensive analysis demonstrates the development and utilization of climate resources of Cheng county and the effect of meteorological disasters of agroforestry and countermeasures, based on the application of heat index and water index and comprehensive index will become county was divided into three climatic regions and two climatic subregions, namely ⅠA warm semi-humid zone grain economic crop areas,ⅡBa temperate humid climate grain economic forest area, ⅡBb mild and humid climate agriculture and forestry sub region, ⅡBc warm and mild semi-arid agricultural economy area, ⅢC temperature cool moist forests, pastoral areas.

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Soil fertility status and evolution characteristic in tobacco-growing areas of Hubei province

XIE Yuan-yuan, SI Guo-han, LI Fang-ming, CHEN Tao, LIU Sheng-gao, LI Bin, DAI Hui-juan, LI Jia-ying

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 39-47. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.009

Abstract （329）

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By analyzing the nutrient indexs of 3 401 soil samples collected from the main tobacco-producing areas of Hubei province in 2012, and comparing with the relevant data in 2002, the soil fertility status and its evolution characteristics in the tobacco-growing areas of Hubei province were studied. The results showed that the average soil pH in tobacco-producing areas of Hubei province in 2012 was 6.2, belonging to the weak acid level. The average contents of soil organic matter was 24.5 g/kg, and the average contents of soilalkali-hydrolyzed nitrogen, available phosphorus and available potassium were 147.6, 36.9 and 239.1 mg/kg, respectively, and the soil organic matter content was moderate, while soil alkaline nitrogen, available phosphorus and available potassium were high to abundant levels; Compared with 2002, the soil pH in the tobacco-producing areas of Hubei province showed no significant change except that in Enshi city, but the acid area increased; Soil organic matter decreased in different degrees, especially in the tobacco-producing areas of Shiyan city. In 2012, soil available phosphorus and available potassium increased significantly, which was 115.3% and 57.7% higher than that in 2002. The average values of soil exchangeable calcium, available zinc and available copper in different tobacco areas of Hubei province in 2012 were 2 105.3 mg/kg, 1.6 mg/kg and 1.1 mg/kg, respectively, which are all at a high level, while soil water-soluble chlorine and soil water-soluble boron were relatively low, and the average values are 9.8 mg/kg and 0.4 mg/kg, respectively. It can be seen that in the future production of tobacco, the application of boron fertilizer and chlorine fertilizer should be carried out scientifically and rationally according to the soil fertility conditions.

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Characteristics and influencing factors of natural lake level change of Hongze lake in 1988—2017

ZHAO Yu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 48-53. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.010

Abstract （359）

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Using Landsat TM/ETM+ remote sensing image as data source, based on GIS and RS technology, using annual change rate and lake change intensity index and other methods to quantitatively analyze the change trend and characteristics of Hongze lake in 1988—2017. By collecting the meteorological data and artificial conservation data of Hongze lake in 1988—2018, combined with the local actual situation, the main causes of area change were studied in depth. ①From 1988 to 2017, the natural lake surface of Hongze lake showed a trend of shrinking-stable-shrinking. In the past 30 years, it decreased 125.81 km², the relative shrinking rate was 9.88%, and the intensity change index （Q） was -0.34. In comparison, the third stage （2009—2017） decreased significantly, followed by the first stage （1988—2000）, and the second stage （2000—2009）, which was relatively stable, with Q of -0.61, -0.24 and -0.13, respectively. In terms of regional location, the change of Hongze lake area was mainly concentrated in the west of the lake area, followed by the northeast. ②Both temperature and precipitation factors are linearly related to the change of lake level, and the overall increase of temperature in 1988—2018 promotes the increase of evaporation; The change of precipitation also leads to the decrease oflake supply. ③From 1988 to 2017, the area of artificial aquaculture expanded 20 times, which directly led to the loss of 5.0% of the natural lake. ④During the change of lake stage, policy change and flood disaster are also the driving factors that affect the change of the natural lake surface of Hongze lake. From 1988 to 2017, the natural lake surface of Hongze lake basically showed a trend of gradual shrinking. Meteorological factors, policy changes and flood and waterlogging disasters caused the lake surface to shrink, but purse seine farming was the main reason for its change.

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Distribution characteristics of soil organic carbon and its influencing factors in different swamp wetland types in arid region

CHEN Tao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 54-62. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.011

Abstract （407）

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Taking wetlands in arid areas as the research object, the distribution characteristics and influencing factors of soil organic carbon in three types of wetlands： Herb marshes, seasonal salt-water marshes and inland salt marshes were studied. The results showed that the soil organic carbon content of the 0~100 cm soil layer of herbaceous marsh, seasonal saltwater marsh and inland salt marsh were 1.19~49.03, 1.62~101.14, 2.61~23.77 g/kg, respectively. The organic carbon content of seasonal saltwater marshes was significantly higher than that of herbaceous marshes; The soil organic carbon content of herbaceous marshes was significantly positively correlated with soil moisture, total nitrogen, and N/P. Seasonal saltwater the soil organic carbon of marsh wetlands was significantly correlated with soil moisture, bulk density, total nitrogen and N/P, and pH showed a very significant negative correlation. Inland salt marsh soil organic carbon was significantly correlated with total nitrogen was extremely significant positive correlation. Combined with the principal component analysis, three types of wetland F₁ contribution rate was highly correlated with soil moisture. It was proved that soil moisture was the key leading factor affecting the accumulation of soil organic carbon.

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Study on the response of Urechis unicinctus and amylase activity under Cd²⁺ stress

WANG Shu-yang, WANG Yue-chen, DU Xing-lin, MU Jin-hua, LYU Dong-wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 63-66. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.012

Abstract （391）

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Used hydrostatic test, cadmium ions solution （0, 0.2, 0.5, 0.8, 1.1 mg/L） prepared with deionized water at a water temperature of （10 ± 2） ℃ was used for 48 h acute toxicity test to Urechis unicinctus. The results showed that the activity of amylase in the body cavity of Urechis unicinctus was simply negatively correlated with the concentration of Cd²⁺ in the living water body of Urechis unicinctus, and with the increasing concentration of Cd²⁺ in the exposed water, Urechis unicinctus showed varying degrees of stress change.

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Screening of high-efficiency and low-toxicity disinfectant against cucumber diseases in Wuhan city

WU Ren-feng, XIE Biao-hong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 67-70. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.013

Abstract （325）

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21 kinds of low-toxicity disinfectant commonly used in production were selected, aiming at three major diseases of cucumber, such as cucumber downy mildew, cucumber angular leaf pot, cucumber corynespora target leaf spot. The experiment of chemical control was carried out. The results showed that 100 g/L cyazofamid SC, 500 g/L fluazinam SC, 72% cymoxanil-mancozeb（Curzate） WP and 75% chlorothalonil WP, 687.5 g/L fluopicolide-propamocarb（Yin Fali）SC, 722 g/L propamocarb hydrochloride（Pulike）AS could be used to control cucumber downy mildew. 20% zinc-thiazole SC,33.5% quinoline copper SC, 3% zhongshengmycin AS could be used to control cucumber angular leaf pot. 75% trifloxystrobin-tebuconazole WDG, 40% difenoconazole-prochloraz EW, 12.5% myclobutanil EC could be used to control cucumber corynespora target leaf spot.

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Effects of different fertilizing quantity on yield and economic benefit of rape varieties

LIU Nian, TANG Tian-ze, FAN Qi-xin, LI Ying-chun, MENG Da-qing, LI Zhi-fan, CHEN Jun, ZHANG Ti-gang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 71-72. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.014

Abstract （333）

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The effects of three different fertilizing quantity on yield and economic benefits of two vegetable and oil dual-use rape（Brassica campestris L.） varieties after picking were studied. Comprehensively calculating the benefits, as avegetable and oil dual-userape variety, Guohaoyou No.5 was better than Mianyou 32. The fertilizing quantity optimal fertilizing quantity for rape after picking was 90 kg/hm².

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Effects of different fertilizer treatments on growth and quality of Chrysanthemum indicum L. developed in different former stub land

ZENG Ye, MA Qing, HAN Zheng-zhou, LIU Hui-hui, XIE Wen-bo

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 73-77. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.015

Abstract （428）

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Using uncultivated field, rice field, rape field for researching, the formula fertilization scheme design was used, the effects of different NPK fertilization treatments on the growth, the whole plant biomass accumulation, and the linarin content changes of Chrysanthemum indicum L. were studied. The results showed that there were significant differences in growth index and effective ingredient content of Chrysanthemum indicum L. cultivated in different cropping systems, and the best treatment for the quality of Chrysanthemum indicum L. was N₂P₂K₂, which achieved the highest quality of Chrysanthemum indicum L. Therefore, the amount of fertilizer of N,P₂O₅,K₂O, respectively was 225.0 kg/hm²,120.0 kg/hm²,150.0 kg/hm².

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Discussion on the way of industrialization development of Capsella bursa-pastoris（Linn.） Medic.

YUAN Chun-xin, TANG Ming-xia, CHENG Yu-jing, WU Hai-yan, WANG Xi-meng, CHEN Jian-jun, GUAN Dong-mei, QIU Xiao-feng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 78-84. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.016

Abstract （432）

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Capsella bursa-pastoris（Linn.） Medic. which is the wild vegetable is popular with consumers of Yangtze River delta and its industrialization prospect is good. But the industrialization of Capsella bursa-pastoris（Linn.） Medic. has much bottleneck problems such as technology, equipment, brand, efficiency, scale, safety, market, circulation. Solving these problems must follow the thinking of the whole industry chain and the general quality control, on the goal of quality lifing, cost reduction, efficiency improving and co-win, with the high-efficiency cultivation and equipment innovation for foundation, clear target location, for industrializationreasearchcontents of the whole set quick-frozen processing equipment based on the intelligent and automaticcharacteristics, the fall-sowing Capsella bursa-pastoris（Linn.） Medic. high yield and quality cultivation technical regulation based on fractionated sowing, the quick-frozen processing technique based on the increasing of glass transition temperature, the cold chain equipment screening and environmental control technology based on the derogation target, the multiparty win-win mechanism based on the goal of multi-industryintegration, promote the Capsella bursa-pastoris （Linn.） Medic. industrialization development by innovating business model, promoting social service, the unification of agricultural materialssupply, constructing cooperative organization, establishing typical function, supportingleading enterprise, developing service organization, lift the whole technology level of Capsella bursa-pastoris（Linn.） Medic.industrialization and the product core competence, increase benefit.

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The imbalance and spatial-temporal evolution of pig breeding in China

YE Wen-xian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 85-95. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.017

Abstract （388）

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Pig breeding was an important part of agricultural production. The panel data of 31 provinces from 2004 to 2018 were selected to measure quantitatively the imbalance degree and spatial-temporal evolution of pig breeding in China. The results showed that there was obvious regional imbalance in pig breeding in China, and the main pig breeding provinces were concentrated in the central region, southwestern region, and southern region. There was a significant positive spatial correlation in pig breeding in China, and the agglomeration effect of pig breeding was more and more obvious. Star provinces mainly included Henan, Guangdong, Guangxi, Sichuan and Yunnan, while skinny dog provinces mainly included Beijing, Tianjin, Shanghai and Tibet. The imbalance degree of pig breeding in the whole country has changed from shrinking first to expanding. The pig breeding in potential area, key area and moderate area was relatively balanced, while the imbalance degree of pig breeding in restricted area was relatively large and expanding. The imbalance degree of pig breeding in the West and East was relatively close and higher than that in the central area. During the research period, the movement of pig breeding center mainly experienced northeast and northwest migration, and the trend of “South pig breeding in North” was obvious, while the phenomenon of “Peacock flying Southeast” was more prominent. From 2004 to 2017, the spatial distance between the focus of pig breeding and the focus of population showed a trend of first increase and then decrease, indicating that the contradiction between supply and demand of pig breeding had evolved from intensification to easing.

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Research on the changes of nutrient elements content in leaves of feeding ramie Emu No.6

TANG Di-luo, XIONG Wei, DING Kun-ming, TANG Shi, WANG Hong-wu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 96-98. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.018

Abstract （356）

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The dynamic changes and the relativity of five mineral nutrients （N, P, K, Ca and Mg） of feeding ramie Emu No.6 leaves were studied during six harvest periods. The results showed that the content, absorption amount and absorption rate of the five nutrients in the leaves were N> Ca> K> Mg> P. The contents of N、P、K、Ca and Mg were different at different harvest periods. The absorption amount and absorption rate of the five elements changed in the same way, and all of them increased first and then decreased. Elements K and N, K and Mg showed remarkable negative relativity and positive relativity（P<0.05）, Ca and K, Ca and Mg showed extremely remarkable positive relativity（P<0.01）.

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Pathological analysis of co-infection of PRRSV,PCV2 and HPS

YANG Yong, JIN Xin, ZHANG Wang-yuan, ZHOU Yi, YAN Wei-wu, XIE Jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 99-102. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.019

Abstract （445）

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8 cases of swine co-infected with Porcine Reproductive and Respiratory Syndrome Virus（PRRSV）, Porcine Circovirus Type 2 （PCV2）, Haemophilusparasuis （HPS） were collected. In order to distinguish the difference in pathological between these three pathogens, gross pathology and histopathological were carried out. The results showed that when the swine co-infected with PRRSV, PCV2 and HPS, the gross pathology mainly included with increasing of fibrous exudation in the thoracic cavity and abdominal cavity, systemic lymph node enlargementing and hemorrhaging, consolidation of lung in tip and heart leaf, hemorrhagic spots on the surface of kidney and white spots on the surface of liver. The histopathological changed mainly showed polyserositis, interstitial nephritis, suppurative pneumonia, suppurative splenitis, suppurative lymphadenitis.

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Optimizing of extraction process of flavonoids from Chenopodium quinoa Willd. seeds

LAI Yun-ping, LIU Xin-chun, WANG Dan-dan, YUAN Jin-e, FENG Zong-yun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 103-106. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.020

Abstract （395）

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In order to optimize the extraction process of flavonoids from Chenopodium quinoa Willd. seeds, as well as to provide theoretical basis for flavonoids exploitation and high flavonoids variety screening. Orthogonal design L₉（3⁴） test including four factors such as ethanol volume fraction, solid-liquidratio, extracting time and extracting temperature was investigated. The results showed that the influence degree of each factor on extraction content of flavonoid from Chenopodium quinoa Willd. seeds were ethanol volume fraction> extraction temperature> solid-liquidratio> extraction time. The optimum extracting conditions were extracting reagent 90% of ethanol, 1∶50（g∶mL） of solid-liquidratio, 50 min of extraction time and 50 ℃ of extraction temperature.

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Optimization of enrichment and purification process of puerarin in the production of pueraria starch

XU Meng-wei, XIAO Li, LI Yi-jin, DONG Xin-rong, LIU De-ming, ZHU Chi-ming

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 107-111. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.021

Abstract （337）

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The method of puerarin enrichment and purification from water discharged by producing pueraria starch using macroporous resin as adsorption material was studied. The saturated capacity of dynamic adsorption （SCD） and elution rate （ER） of fifteen type of resin for total isoflavones of pueraria was investigated. The results indicated that the SCD for total isoflavones of pueraria was 39.41 mg/mL resin and the ER of resin was 91.42% eluted by 70% ethanol aqueous at 1.0 BV/h flow rate. And 4.7 g of crude isoflavones with the content of total isoflavones of 61.67% （UV spectrophotometry） and the content of puerarin of 31.80% （HPLC） could be recovered from 3 L discharge water. A high purity sample which contained 96.37% puerarin was obtained by 5% HCl hydrolysis and recrystallization, and recovery of puerarin was 15.91%. This was the best process optimization for puerarin enrichment and purification from water extracted pueraria starch.

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Optimization and activity determination of total flavonoids from Flammulina velutipes purified by ADS-8 macroporous adsorption resin

WANG Guang-hui, TAN Ji-li, DU Yan-chao, YANG Jing-yi, SHANG Ya-nan, REN Li-na

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 112-115. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.022

Abstract （457）

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In order to determine the optimal conditions for the purification of Flammulina velutipes flavonoids by ADS-8 macroporous resin and the antibacterial and antioxidative properties of flavonoids after purification, the effects of pH and concentration of sample solution on the adsorption properties of flavonoids in resin and the effects of eluent dosage and eluent volume fraction on the analytical efficiency of flavonoids in the case of ethanol as eluent were studied, and the ability of purified flavonoids to inhibit Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Salmonella enteritidis was tested. The ability of scavenging 2,2-diazo-bis （3-ethyl-benzothiazole-6-sulfonic acid） diammonium salt （ABTS·） was detected. The results showed that the optimal conditions for this purification process were that the pH of the sample solution was 5, the concentration of the sample solution was 1.19 mg/mL, the volume fraction of the eluent ethanol was 70%, and the amount of ethanol was 140 mL. Under the above conditions, the purification ratio of flavonoids was 1.84 times. The flavonoids had inhibitory effects on four kinds of reference bacteria. The order of antibacterial ability was Escherichia coli> Salmonella enteritidis>Staphylococcus aureus>Bacillus subtilis. The results showed that the total flavonoids of Flammulina velutipes had the ability of scavenging ABTS·, indicating that it had antioxidant activity, but weaker than vitamin C.

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Determination of the concentration of flue-cured tobacco volatile oils by UV-spectrophotography

WANG Ke-han, XIANG Huan, YANG Yi-de, ZHANG Yong-hui, ZENG Shu-hua, YANG Min-feng, GUO Shi-ping, LIU Lei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 116-120. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.023

Abstract （401）

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To determining the concentration of volatile oils solution extracted from flue-cured tobacco leaves, a method based on UV spectrophotometry was researched. Volatile oils were extracted by simultaneous distillation and diluted with dicholormethane to prepare a set of gradient solutions. Each solution was scanned by UV spectrophotomet. The best working wavelength was selected to establish linear regression equation between the concentration of volatile oil and its ultraviolet absorbance. Results showed that the volatile oils had a large absorption spectrum between 225~245 nm. The best analysis wavelength was 239 nm, the linear relationship was y =0.250 1x + 0.039 3, R²=0.999 3. Its relative error of was less than 0.5%, the limit of detection （LOD） and the limit of quantification （LOQ） of the working curve were 0.153 g/L and 0.464 g/L separately.

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Rapid determination of parathion and isofenphos-methyl residues in plant foods by gas chromatography

MENG Qing-shun, ZHOU Yi-guo, LIU Qiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 121-122. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.024

Abstract （433）

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A method was established for the determination of the content of parathion and isofenphos-methyl in plant food by gas chromatography. The method was validated by FPD detector, external standard method and automatic injection, the results showed that the detection limits of parathion and isofenphos-methyl were 2 μg/kg （1 μL, S/N=3）, the recoveries of parathion and isofenphos-methyl were 86.7%~94.5%, 89.3%~92.6%, the RSD（n=10） of parathion and isofenphos-methyl were 3.86% and 4.23% respectively.

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Design and implementation of kiwifruit quality and safety control system based on internet of things

CAI Jie, YANG Li-xin, LIU Yan-fang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 123-126. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.025

Abstract （395）

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The application of internet of things, big data and other information technologies in kiwifruit production was an inevitable trend of industrial development. Compared with the traditional mode, the intelligent production mode has many advantages such as cost saving, efficiency increasing, precision and environmental protection. the kiwifruit quality and safety control system based on the Internet of things was designed for the kiwifruit intelligent production mode, and the system functions were analyzed in detail, which provided a new idea for the realization of kiwifruit intelligent production mode.

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The application model of digital library scene based on blockchain technology

ZHU Bai, LI Yin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 127-132. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.026

Abstract （599）

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A hierarchicaliy scenario application model architecture of digital library based on the blockchain technology was built, and the key functional components and core service modeles of each layer in detail were introduced, finally the comprehensive and comparative analysis with the traditional digital library application scenarios was made. Results showed that the comparative analysis of the traditional and the blockchain technology service scenarios, the blockchain technology applied to the scenario application of digital library was safe and reliable, and could solve the credibility of digital library resources effectively, enhance the interoperability and scalability of the system architecture, and further realize the safe circulation of the heterogeneous digital resources.

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Optimization model of agricultural products logistics network based on hybrid genetic algorithm

AI Ting-ting

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 133-138. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.027

Abstract （565）

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In order to solve the optimization problem of fresh agricultural products logistics distribution system, considering the characteristics of short shelf life of fresh agricultural products, combined with the overall optimization idea of farm products logistics distribution network model, a cold chain logistics network optimization model based on hybrid genetic algorithm was established. This optimal model aims at the total operation cost of the logistics network, introduces carbon tax policy to analyze the impact of carbon tax on the total cost and carbon emissions, and proves that carbon tax policy can effectively reduce carbon dioxide emissions in the farm products logistics network. The simulation results obtained from the actual case analysis show the applicability of the model, which provides a green location allocation scheme for agricultural logistics enterprises.

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Composition and quality analysis of high molecular weight glutenin subunits in Neimai variety

WANG Xiang-quan, CHEN Xing-hu, HUANG Hui-yue, WANG Ren-quan, YANG Jie-zhi, GUAN Shu-xian, WANG Shi-lin, RONG Fei-xue, WANG Yong-jun, ZHOU Hai-yan, LI Ming, CHEN Xin-yuan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 139-142. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.028

Abstract （334）

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Neimai series is one of the representative varieties of middle gluten wheat in the southwest wheat region, with a total planting area of over 3.3 million hm² in Sichuan and surrounding provinces. The composition of high molecular weight glutenin subunit （HMW-GS） in Neimai varieties was analyzed by SDS-PAGE electrophoresis. It was found that the 5+10 high quality subunit in Neimai varieties was positively correlated with the processing quality of wheat with a frequency of 85.7% and a bread score of 7 points. The noodle score of Neimai varieties was higher than that of main varieties. The breeding of wheat varieties containing 5+10 high quality subunits can not only meet the market demand of middle gluten noodle wheat in southwest China, but also contribute to the improvement of wheat quality in China.

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Programmable base editing efficiency study of CRISPR/Cas9-guided DNA base editors in pig genome

ZHANG Ting-ting, CHEN Tao, LI Yan-li, YANG Man-man, WEI Qiang, WANG Ran, LI Lin, LI Yong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 143-149. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.029

Abstract （279）

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The CRISPR/Cas9-based BE3（Cytosine base editor,CBE） and ABE7.10（Adenine base editor,ABE） were used to analyze and study the editing efficiency of target gene loci in pig genome. Four porcine genomic target gene loci gRNA expression vectors were designed, synthesized and constructed, which were co-transfected into PK15 cells with CBE or ABE, respectively. The PK15 cells were further cultured for 48 h, and single-base replacement efficiency and indels incidence were determined by second-generation sequencing technology. The results showed that the active editing Windows of the single base editing system BE3 and ABE7.10 were 5 nucleotides and 4 nucleotides, respectively; Both BE3 and ABE7.10 were mainly contributed to single base conversion but not indel; The two sets of single-base editing systems showed certain preference for base replacement in pig genome. In the CMAH, MC1R（1-2） and MC1R（2-1） gene editing Windows, the efficiency of C→T was 2.2%, 0.4% and 1.3%, respectively; The efficiency of A→G in pig GGTA and MSTN-2 was 1.4% and 1.4%, respectively. It is shown that both BE3 and ABE7.10 can perform effective single base substitutions for genomic target sequences in pig cells.

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Study on the spatiotemporal evolution characteristics of rural hollowing in Changsha-Zhuzhou-Xiangtan region

NING Jin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 150-155. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.030

Abstract （394）

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Taking 23 counties （cities and districts） in Changsha-Zhuzhou-Xiangtan region of Hunan province as the research area, the measurement framework and evaluation index system of rural hollowing were constructed from three aspects of land, population and economy. The evaluation index data were processed by using extremum method and variation coefficient method, and the spatiotemporal evolution characteristics and influencing factors of rural hollowing in the Changsha-Zhuzhou-Xiangtan region from 2009 to 2018 were analyzed. The results showed that, the main factors affecting the rural hollowing were resource environment, social economy, population, management and policy.

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Study on the relationship between urbanization, industrial structure and agricultural economic growth

MA Qian-li

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 156-159. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.031

Abstract （336）

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Using the ratio of urban population to total population, the ratio of added value of tertiary industry to secondary industry and total agricultural output value from 2000 to 2018 as sample data, based on VAR model and cointegration test, this research studied the interaction among urbanization, industrial structure and agricultural economic growth. The results showed that urbanization and industrial structure have significant impact on agricultural economic growth. The impact of urbanization on agricultural economic growth was positive, and the impact of industrial structure on agricultural economic growth was negative. The influence of urbanization and industrial structure on agricultural economic growth should be considered comprehensively. With the passage of time, the contribution rate of urbanization to the variance of agricultural economic growth increased significantly; the contribution rate of industrial structure to the variance of agricultural economic growth increased slightly; the proportion of variance fluctuation caused by the factors of agricultural economic growth decreased significantly. We need to focused on the impact of urbanization on agricultural economic growth. Urbanization, industrial structure and agricultural economic growth had a stable long-term equilibrium relationship. In the long run, the government should ensure the coordinated development of urbanization, industrial structure and agricultural economic growth.

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Analysis on the relationship between R&D investment and core competitiveness of agricultural companies

MENG Qing-jun, GU Jia-le

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 160-164. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.032

Abstract （290）

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“Agriculture, rural areas and farmers” was a social issue that the Chinese government and scholars pay close attention to. The core competitiveness of agricultural companies play a very important role in China’s agricultural development. It was of great theoretical significance and application value to study the relationship between R&D investment and core competitiveness of agricultural companies. This study put forward three hypotheses from the relationship between R&D investment and core competitiveness, selected the data of 40 agricultural listed companies in a share from 2014 to 2018 as the sample for empirical analysis, and concluded that R&D investment of agricultural companies was significantly positively correlated with core competitiveness. Finally, the symbiosis theory was used to give solutions from the perspectives of agricultural companies, government, scientific research institution, securities companies and investors.

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Study on the evaluation of the benefit of poverty alleviation fund investment in deep poverty counties：Take Danfeng county in Qinba mountain area as an example

GUO Meng, WANG Yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 165-168. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.033

Abstract （314）

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Taking Danfeng county in Qinba mountain area as an example, this study used regression equation and Pearson correlation coefficient method to evaluate and analyze the input and output data of poverty alleviation from 2013 to 2019. It was found that the direct benefit of poverty alleviation fund investment was very significant, and the direct effect on the incidence of poverty was higher than the effect on disposable income of rural residents. The types of poverty alleviation fund investment had a significant impact on the income and expenditure of farmers construction. Based on this, this study put forward countermeasures and suggestions to optimize the allocation structure of poverty alleviation funds, innovate the financing mode of poverty alleviation funds, and improve the evaluation mechanism of poverty alleviation funds, so as to provided reference for the poverty alleviation of poverty-stricken counties and the government to adjust the investment policies of poverty alleviation funds.

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Analysis on sisutation of world minor grain production and trade

LI Yuan-xin, QU Jia-jia, ZHANG Hui-jie, HU Xiang-dong, MA Ji-liang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 169-175. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.034

Abstract （474）

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Understanding the situation of world minor grain production and trade in the past 10 years was very important to control the changes of world minor grain production and trade, and to study and judge the future pattern of world minor grain production and trade.Based on the statistical data of world minor grain production and trade in FAOSTAT database, the evolution of world minor grain production and trade situation in recent 10 years was analyzed, and the characteristics of world minor grain production and trade situation were summarized. It was found that in the past 10 years, the world's grain yield per unit area has increased, the total output has been increasing, and the total import and export trade volume has also shown an upward trend, but the price of import and export trade has decreased. The production concentration and trade concentration of single variety of minor grain were relatively high in the world. Major production and trade varieties of minor grain were not expected to change greatly. Major production areas and major trade areas were also expected to have no major changes, but the latter was partially adjusted. There were different production characteristics and trade characteristics among different varieties of gramineous grains, edible legume grains and different varieties in the world.

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Research on the coordination degree between the social economic development and cultivated land area changes in Yiwu city

LI Ru

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 176-180. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.035

Abstract （310）

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Taking Yiwu city of Zhejiang province as the research object, this research used the coordination degree model to analyze the relationship between the social economic development and cultivated land area changes in Yiwu city from 2006 to 2018, and used the linear regression model to predict the coordination degree between the social economic development and cultivated land area changes in 2023 and 2028. The results showed that there was an obvious negative correlation between the social economic development and cultivated land area changes in Yiwu city from 2006 to 2018. The coordination degree of the two was low. In recent years, it was in a harmonious state and had not reached the coordination level. The coordination degree between the social economic development and cultivated land area changes will not change greatly in the next 10 years. Finally, some suggestions were put forward to promote the coordinated development between the social economy and cultivated land resources, so as to realize the sustainable development of the social economy and the rational allocation of the cultivated land resources.

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Research on the construction and measurement of evaluation index system of agricultural high-quality development in Hubei province

ZHOU Xin-hao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 181-184. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.036

Abstract （419）

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Combined with the seven characteristics of agricultural high-quality development, the evaluation index system of agricultural high-quality development was constructed, and the agricultural high-quality development level of 13 cities and prefectures in Hubei province was measured. The results showed that, there were regional differences of the agricultural high-quality development in Hubei province, among which Jingmen and Wuhan were in the forefront and play the leading role in demonstration; as traditional agricultural cities, Xiangyang, Jingzhou, Huanggang and Yichang were accelerating the transformation and upgrading to the strong modern agricultural city; other cities and prefectures were relatively backward and had different shortcomings.

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Comparative study on environmental protection behavior of urban and rural residents in China：Analysis based on CGSS 2013 data

CHENG Meng-guang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 185-190. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.037

Abstract （354）

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Based on CGSS 2013 data, the differences and influencing factors of environmental protection behavior of urban and rural residents in China were comparatively analyzed. The results showed that,the practice level of environmental protection behavior of urban and rural residents was low, the personal and public environmental protection behavior of urban residents was higher than that of rural residents; the positive effect of environmental problem cognition on personal environmental protection behavior of urban residents was greater than that of rural residents, and the positive effect on public environmental protection behavior of rural residents was greater than that of urban residents; environmental knowledge was helpful for urban and rural residents to take personal environmental protection behavior,but had negative effect on public environmental protection behavior of urban residents; environmental protection job satisfaction was conducive to the implementation of environmental protection behavior of urban and rural residents, but had different effects on personal environmental protection behavior and public environmental protection behavior of urban and rural residents.

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Risk assessment of exhibition logistics exhibits transportation based on fuzzy fault tree

LIU Rui-xian, CAO Run-feng, LIU Jing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 191-197. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.038

Abstract （386）

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Exhibition logistics exhibits had transportation risks, and the traditional risk assessment methods were difficult to complete the task efficiently. A risk assessment method based on fuzzy fault tree was proposed based on the case of the 3rd China Garden and Garden Horticulture Exhibition in 2020. The exhibition logistics exhibits transportion failure fault tree was established, the consistent decision was used to synthesize expert opinions, the de-fuzzy output was used to get the probability of the bottom event, and the weak links of the top event were obtained by comparing the importance of fuzzy probability. The results showed that the weak links that affecting the transportion failure of exhibition logistics exhibits were mainly the complex road conditions of transportation lines, fatigue driving of transportion vehicle drivers and poor safety awareness of transportation vehicle drivers.

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Evaluation of regional public brand value of agricultural products from the perspective of financial analysis

PANG Jing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 198-202. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.039

Abstract （447）

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With the further advancement of the rural revitalization strategy, how to evaluate the value of the regional public brand of agricultural products has become an important topic of agricultural brand research. At present, there was a lack of specific and in-depth research on financial elements and brand value of agricultural products. Based on the perspective of financial analysis, the evaluation index system of regional public brand value of agricultural products was constructed. Taking Ankang selenium rich tea in Shaanxi province as an example, the analytic hierarchy process method and fuzzy comprehensive evaluation method were used to comprehensively evaluate the Ankang selenium rich tea, in order to provide basis for stakeholders to make effective decisions.

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Study on the survival dilemma and countermeasures of older unmarried men in rural areas：Based on the questionnaire survey of Zhumadian city in Henan province

SUN Zhong-gen, LI Yu-juan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 203-207. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.040

Abstract （464）

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Older unmarried men were one of the phenomena of the imbalance of population gender structure, and the social risk and harm brought by it can not be ignored. Especially in rural areas, under the influence of the traditional patriarchal ideology and the outflow of a large number of young women, there were more and more unmarried men. Due to the lack of support and dependence from their own families, most of them were faced with the dilemma of lonely life, which brought hidden dangers to the healthy and stable development of local society. Based on the questionnaire survey of older men in rural areas in Zhumadian city of Henan province,this study systematically investigated the survival conditions of older unmarried men in rural areas, clarified the survival dilemma and its cause, and put forward countermeasures and suggestions to improve the survival conditions of this group.

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Analysis on poverty reduction effect and influencing factors of land use patterns of ecological immigrants in Ningxia

CHEN Wan-ming, CHANG Dong-ni, TAI Xiu-jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 208-212. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.041

Abstract （328）

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Based on the survey data of 432 migrant households in two cities of Ningxia Hui Autonomous region, this study used the FGT poverty measurement method and binary logistic regression method to measure and analyze the poverty reduction effect and influencing factors of land use patterns of migrant households in resettlement areas. The results showed that, compared with the traditional family management of land, the overall poverty level of the new type of collective lease of land was relatively low; the financial capital and psychological capital were the most significant factors influencing the migrant households to accept the new type of collective lease of land,followed by material capital, human capital and social capital,and the influence of natural capital was not very significant. Finally, some suggestions were put forward in order to provide reference for the land use and livelihood restoration of the resettlement areas.

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Research on the innovation of rural cultural governance system in southern Shaanxi

HU Ming-han

HUBEI AGRICULTURAL SCIENCES 2020, 59 (18): 213-216. DOI: 10.14088/j.cnki.issn0439-8114.2020.18.042

Abstract （334）

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Rural culture was an important part of rural revitalization. By analyzing the current situation, existing problems and difficulties of rural cultural governance in southern Shaanxi, it was concluded that a comprehensive rural cultural governance system in southern Shaanxi must be established. On this basis, this study proposed to establish the rural cultural governance system in southern Shaanxi, which was guided by advanced ideas, comprehensive service, multi subject coordination and long-term governance guarantee, so as to provide reference and reference for the rural cultural governance in southern Shaanxi and even the whole country.

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Exploration of strategies and ways of rural revitalization under the integration of culture and tourism：Based on the experience of Huzhou, the birthplace of “Two Mountains” theory

SHEN Chen-shi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 5-8. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.001

Abstract （486）

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The integrated development of culture and tourism provides new opportunities for upgrading rural tourism, realizing a new round of innovative development and better promoting rural revitalization. From three aspects, this paper analyzed that cultural and tourism integration has injected new momentum into rural revitalization; it also discussed the successful experience of Huzhou city, Zhejiang province, where the concept of “Two Mountains” was born. The practical exploration experience of Huzhou city shows that in the practice of promoting rural revitalization by cultural and tourism integration development, all localities must adhere to the planning guidance, integrated development, innovation prying, brand driven and element linkage, which can effectively break the bottleneck of the integration of rural culture and tourism, promote the deep integration of rural culture and tourism, improve the quality and efficiency. On this basis, the strategies and path selection of deepening cultural and tourism integration and helping rural revitalization were put forward.

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Rethinking on the theoretical logic, 100-year practice and path of rural revitalization：Based on modernization of human

ZHANG Zhi-hui, HUANG Ping, WANG Hua, YANG Ya-dong, JIANG Zi-chun, LIU Jian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 9-14. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.002

Abstract （436）

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The realistic proposition, core direction and theoretical logic that the rural revitalization strategy pays attention to must be reviewed and reconsidered under the historic general task of modernization. China’s modernization have corrected Smith’s tendency to replace the modernization of men with modernization of things, it pays attention to the key role of system construction in social structure and highlights the modernization of men in Hegel’s path, which has been weakened by the suppression of system modernization. In essence, China’s modernization is how to realize the modernization of peasants in the big country who are “poor, stupid, weak and private” it is a process of continuous rural revitalization, which is based on the modernization of “things” and takes the system modernization as intermediary and the modernization of men as the most essential aspect. It has experienced three stages of nearly one hundred years of practice, which are the hard exploration of “rebuilding the new people” by the rural construction school and “turning over to be the master” by the revolutionaries who wanted to smash the old world, the rapid evolution of modernization led by the country and the implementation of the rural revitalization strategy. The rural revitalization strategy follows the theoretical logic of modernization. In the process of the strategy implementation, we need to deal with the “three relationships” and unblock the “three approaches”.

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Evaluation on the development efficiency of green economy in Huzhou city under the practice of “two mountains” theory

LI Na

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 15-22. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.003

Abstract （393）

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Based on the panel data of the three counties and two districts of Huzhou city from 2008 to 2017, the green economy development efficiency evaluation indicators were selected. The DEA-SBM model was used to scientifically analyze the current status of the efficiency of Huzhou’s green economy development from the two dimensions of time and region. The results showed that the efficiency value of green economy development in Huzhou city fluctuates significantly over time, and there were varying degrees of difference in efficiency values between regions. Then an indicator system from five aspects： green production, green life, economic structure, government, and technological level was designed,the Tobit model was used to perform regression analysis on the relevant factors that affect the efficiency of green economy development in Huzhou city. Finally, an optimized path to promote the better development of Huzhou’s green economy was proposed, in order to provide ideas and references for Huzhou and other cities to further practice green economic development.

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Investigation on sustainable development of crawfish-rice industry in Hubei province

LU Guo-liang, XU Miao, WANG Ben-fu, CHENG Jian-ping, CHEN Jie

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 23-26. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.004

Abstract （316）

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“Crawfish-rice co-farming” has become a leading industry in Hubei province because of its remarkable economic and ecological benefits and broad market prospects. After investigation of main production areas of crawfish-rice,the existing problems in crawfish-rice industry development were analyzed in Hubei,and the corresponding suggestions of industry development were proposed.

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Innovation and practice of cooperative extension mechanism of ratoon rice industry in Hubei province

CAO Peng, LIU Dan, ZHANG Jian-she, WANG Ben-fu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 27-30. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.005

Abstract （375）

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Developing ratoon rice industry is an important means to realize green and sustainable development of agriculture in Hubei province. There are many restrictive factors in the traditional ratoonrice industrywith the change of production situation and conditions.In order to improve the development level of ratoon rice industry, multiple units in Hubei province were organized and cooperated to carry out research on ratoon rice bottleneck technology, the organic combination of ratoon rice technology innovation and industrial development was promoted, and the effective docking of technical service and industrial demand was established, and the collaborative promotion mechanism of “one main body and multiple elements” was constructed. Good results have been achieved in the cooperative promotion of ratoon rice industry, with significant economic, social and environmental benefits.

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Research on the development path of traditional Chinese medicine industry in Enshi prefecture

NI Ni, HE Rong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 31-35. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.006

Abstract （347）

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Based on the comprehensive analysis of the development foundation of traditional Chinese medicine industry in Enshi prefecture, the influencing factors that restricting the development of traditional Chinese medicine industry in this region were systematically summarized, and the path and countermeasures that were beneficial to the development of traditional Chinese medicine industry in this region were put forward, so as to further enrich the content and vision of practical research on rural revitalization strategy in ethnic areas.

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Evaluation of output benefit of geographical indications agricultural products in Shaanxi province based on fuzzy comprehensive evaluation method

LI Na-na, ZHOU Xiao-ting, DENG Shu-hong, ZHANG Hong-xia

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 36-39. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.007

Abstract （360）

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The factors which affect the output efficiency of Shaanxi geographical indication agricultural products were divided into internal factors and external factors, the fuzzy comprehensive evaluation method was used to evaluate the output benefits of geographical indication agricultural products in Shaanxi province from seven aspects of quality specification, brand value, geographical location, government decision-making, market risk, business entities and consumer preference. The results showed that the highest yield of agricultural products with geographical indication in Shaanxi province was 0.375 and the higher yield was 0.226, which had obvious output benefit. At the same time, some suggestions and measures were put forward to promote the development of geographical indication agricultural products in Shaanxi province.

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The characteristics and implications of spatio-temporal coupling between geographic indications agricultural products and agricultural economy development in Guangxi

YAN Zhi-qiang, YAN Qin-qiang, PENG Ding-xin, SA Chu-hang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 40-46. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.008

Abstract （453）

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Quantitative analysis and GIS spatial analysis methods were used to analyze the spatio-temporal coupling characteristics of the change of quantity and output value of geographic indications agricultural products and the development of agricultural economy of counties in Guangxi from 2005 to 2015. The results showed that, from 2005 to 2015, the quantity of geographic indications agricultural products in Guangxi counties showed a growing trend, with the growth rate increasing first and then decreasing; From the perspective of space, the growth of geographic indications agricultural products was mainly concentrated in the east, south and northwest of Guangxi. From 2005 to 2015, the output value of Guangxi’s geographic indications agricultural products showed a continuous growth trend, with the growth rate gradually decreasing, and the spatial distribution of the growth was basically consistent with the quantitative distribution. In 2005, 2010 and 2015, the county agricultural economy in Guangxi presented a pattern of unbalanced development, the agricultural economy of different gradients gathered and distributed in space. The regions with good agricultural economic development were all in the six basins （plains） and the border areas, while the regions with less development were mainly concentrated in the northwest. The number of counties that geographic indications agricultural products was few, but the agricultural output value was high, and geographic indications agricultural products was few and the agricultural economy laged behind was relatively more. From 2010 to 2015, the geographical indications agricultural products industry in Guangxi was in a stage of rapid development, and the growth rate was much higher than that of agricultural economy, but the growth rate was different among counties. Finally, according to the spatio-temporal coupling characteristics of geographic indications agricultural products and agricultural economic development, some suggestions on developing geographic indications agricultural products to promote the prosperity of rural industry were put forward.

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Research on the influence of new media marketing on the purchase behavior of Yantai Giant Cherry brand：Empirical analysis based on binary logistic regression model

WANG Han, LI Mei-gui, GAI Yu-heng, ZHANG Yu-jing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 47-50. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.009

Abstract （413）

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Yantai Giant Cherry was selected, a well-known regional brand of agricultural products, as the object. Based on the survey data of 262 consumers in Shandong province, a binary logistic regression model and SPSS 20.0 soft was used to empirically analyze influence factors of the new media marketing on consumers purchase behavior of Yantai Cherry. The results showed that consumer gender, frequency of new media use, usefulness of new media, interactivity of new media, and credibility of new media had a significant positive effect on consumers purchase behavior of Yantai Giant Cherry; Consumer age, education, income, andusability of the new media had no significant impact on its purchase behavior. Finally, related policy recommendations were put forward to promote the new media marketing to promote regional brands of agricultural products.

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An empirical analysis on influencing factors and the measurement of tea export quality in China

LIAO Liang-mei, LU Tian-run, SHU Jia-yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 51-55. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.010

Abstract （424）

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Based on the data of the United Nations database on HS code 0902 （2009—2017）, the similarity, technical complexity and unit price of tea export of 10 major tea exporting countries in the world, including China, Kenya, Sri Lanka, India, Indonesia, Vietnam, Argentina, Uganda, Malawi and Tanzania, were measured. It was proved that the quality of Chinese tea export was high.And an empirical study on the influencing factors of Chinese tea export was maked,the results showed that the income level of tea exporting countries, the per capita income level of importing countries, the strength and geographical location of tea exporting enterprises were positively correlated with the quality of tea export; technical barriers had a positive role in promoting the quality of Chinese tea export; the fierce competition in the world market promoted local enterprises to carry outexport tea quality upgrade. Finally, according to the influencing factors, some suggestions to improve the quality of Chinese tea export from the aspects of technology and market were put forward.

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Exploration on the development path of characteristic villages under the background of rural revitalization：Taking Xujiayuan in Jiangning district of Nanjing as an example

ZHANG Xiao-meng, QU Zhong-qiong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 56-60. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.011

Abstract （480）

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Based on the background of rural revitalization and the perspective of tourism industry development, taking Xujiayuan in Jiangning district, Nanjing city as an example, the development path of Xujiayuan characteristic villages was explored through field investigation. the development status and the existing problems were analyzesd, countermeasures and suggestions were put forward,so as to provide reference for other characteristic villages to actively integrate into the whole area tourism situation.

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Problems and countermeasures in the development of E-commerce poverty alleviation platform of poor area

DENG Jian-gao, ZHAO Cun-xue, YANG Yu-xuan, WANG Yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 61-63. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.012

Abstract （345）

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Based on the analysis of E-commerce poverty alleviation model and characteristics, China social poverty alleviation network was took as an example, and the problems existed in the E-commerce poverty alleviation platform were analyzed. Firstly, the E-commerce poverty alleviation platform of poor area was weak in using big data technology, the platform did not make full use of big data. Secondly, the platform had weak brand awareness. Thirdly, business training needed to be improved urgently. At last, four countermeasures were put forward. The platform should strengthen the combination of operation and big data, improve the business literacy through training, mine regional product characteristics deeply, and create unique brands for each area.

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Research on high quality growth path of agricultural economy in Shaanxi province

CHEN Wei-feng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 64-66. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.013

Abstract （465）

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Agricultural economic growth is not only a hot issue in the field of agriculture, rural areas and farmers, but also a major issue related to people’s livelihood. As a major agricultural province, Shaanxi province is rich in agricultural resources, and agricultural economic growth plays a significant role in the layout of macro-economic growth. So, the research on high-quality agricultural economic growth in Shaanxi province is of great significance to its economic development. Firstly, the connotation of high-quality growth of Shaanxi agricultural economy was defined; Secondly, it puts forward that the high-quality growth of Shaanxi agricultural economy was faced with the problems of unreasonable agricultural industrial structure, imperfect agricultural industrial chain, insufficient investment in agricultural human capital and low utilization rate of modern agricultural science and technology; Finally, combined with the situation of Shaanxi province,the path selection of high-quality growth of Shaanxi agricultural economy was expound.

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Study on spatial-temporal differentiation of urban land use efficiency in Shandong province

FENG Xuan-feng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 67-72. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.014

Abstract （345）

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Based on the construction of the comprehensive evaluation index system of urban land use efficiency in Shandong province, the weight of each index was determined by entropy method. The time sequence characteristics of the comprehensive land use efficiency of 17 prefectural-level cities in Shandong province from 2006 to 2016 were studied by using the comprehensive index model, and the spatial difference of the coordination degree of land use efficiency in each city was analyzed by using the coupling coordination model. The results showed that the land use degree of each city was quite different from 2006 to 2016, and the comprehensive land use benefit presented the situation of “high, middle and low” stratification and showed different development trends in time, and the differences were mainly reflected in the indicators related to economy. The coupling coordination degree between the social and economic benefits of land use and the ecological and environmental benefits of each city was very different. Among them, Qingdao city had the highest coordination degree, reaching 0.85, while Heze city had the lowest coordination degree, only 0.42. In terms of space, the coordination degree of eastern coastal cities of Shandong was relatively high, and that of western inland cities was relatively low.

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Evaluation on carrying capacity of land resources and suitability of construction land in Jinyang county

WU Zheng-fei, ZHAO Qi-hua, ZHANG Chao-jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 73-77. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.015

Abstract （366）

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The southwestern mountainous cities are characterized by complex topography and geomorphology, sensitive ecological environment, tight urban construction land, high population density, and relatively lagging economic development. Urban development and construction are greatly restricted. To provide a reference for the more scientific formulation of urban development planning, the suitability evaluation of construction land and the carrying capacity evaluation of land resource based on the mean square error decision-making method in the representative mountainous city of Jinyang county in the southwestern region, were carried out.The results showed that land suitability score was 7.87, which was suitable for the construction area. The land resource carrying capacity had gradually decreased from good state to crisis state in the decade from 2007 to 2016, and land resource carrying capacity sharply decreased during the period from 2008 to 2009 due to the population increase. In the evaluation of land resource carrying capacity, the land resource carrying potential were positively correlated with the carrying capacity of land resources, and the overall trend showed a downward trend; The land resources carrying efficiency was negatively correlated with the carrying capacity of land resources, and the overall trend was upward. The suitability of construction land in urban area was good, but the edge of urban area was poor. Two suggestions were put forward：Accelerate the construction of the new city, and carry out reasonable population planning and population evacuation for the old city; Pay attention to the protection of land resources, especially the planned use of suitable land for construction, to achieve the purpose of sustainable development.

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Analysis of the present situation of facilities agricultural land in Laixi city based on RS and GIS

YANG Hai-bin, WANG Chao-jie, GAO Ya

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 78-81. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.016

Abstract （352）

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In recent years, the development of agricultural modernization has been speeding up, the high economic benefits of facilities agriculture has attracted more and more people choose to develop facilities agriculture. Facility agricultural land is becoming more and more,which brings some difficulties for the use and management of facilities. Based on the RS and GIS techniques, the spatial distribution and economic benefits of plastic greenhouses, greenhouses and small sheds in the agricultural land of Laixi city in Shandong province were statistically analyzed. The results show that, although the development of facility agriculture in Laixi city was increasingly fast, but the distribution was relatively fragmented,and it has not yet formed a large-scale business structure. There is still much space for development of facilities agriculture of Laixi city.

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Empirical analysis on the economic benefits of farmland transfer in the main grain producing areas：A case of Qitai county

WANG Li, YANG Jun-xiao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 82-85. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.017

Abstract （333）

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Studying the economic benefits of farmland transfer in the major grain-producing areas can provide reference for the development of farmland transfer. The economic benefits of farmland transfer in the study area from the perspectives of farmers’ economic behaviors and marginal benefit were analyzed by the C-D production function. The results showed that, from the farmers’ economic behavior, the farmers’ behavior was economic, the farmland transfer had increased agricultural productivity and produced economies of scale; From the marginal income of agricultural land, the marginal benefit of the farmers who not involved in farm land transfer was greater than that of involved farmers, which showed the involved farmers transfered the farmland marginal income into the production and operating income. Agricultural land transfer economic benefits in studying area are significant, in the process of guiding the transfer of agricultural land, the government should focus on the development of small farmers’ families and improve the level of agricultural technology, combine the local conditions to provide quality integrated services for small farmers’ families.

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Research on the consolidation potential of hollow village residential area based on realizable point： A case of Nanyang village

HU Rong-zheng, WANG Yun-ping

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 86-90. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.018

Abstract （350）

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At present, the measurement method of consolidation potential of hollow village residential area can not measure the ecological environment improvement potential of hollow villages, then impacting on rural revitalization. To solve this problems, this paper adopted normative analysis method to redefine the micro-definition of rural residential consolidation potential based on realizable angle, in order to express the importance of ecological environment improvement potential. On the other hand, the comparative analysis method was adopted to analyze the connotation composition of potential under various methods of rural residential potential measurement, and according to the specific situation of the rural residential hollow in the study area, a method of calculating the realizable consolidation potential based on the classification method of ecological-living-industrial land was proposed. The ecological land category, other land category, the safe idle category, the unsafe use category, the unsafe idle category and the unsafe abandoned category in the production or living land were identified as the consolidation potential. According to the realizable degree of land consolidation, it was divided into three kinds of potentials： easy realization potential, realizable potential and difficult realization potential. In the end, the residential consolidation potential of Nanyang village of Shanxi province was calculated for demonstration. The results show that this calculating method and micro-definition of the potential of rural residential consolidation can analyze the realizable point of potential components and measure the actual potential, and also can protect the village internal ecological environment and promote the rural ecological revitalization.

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Study on coordination of urbanization, economy and environment in Chengdu metropolitan area

HOU Lan-gong, ZHANG Xiang, ZHAO Chun-rong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 91-98. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.019

Abstract （386）

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Taking the Chengdu metropolitan area as the research area, the urbanization level, economic development quality and ecological environment of the nine cities in Chengdu metropolitan area from 2005 to 2016 in terms of urbanization rate, GDP ratio of tertiary industry and ecological environment were calculated and analyzed. Based on the situation, the coordination degree model was used to analyze the coordination of the three. The results showed that the urbanization rate of the urban area was continuously improved, the quality of economic development continued to improve, and the quality of the ecological environment was generally declining. The degree of coordination among urbanization level, economic development quality and ecological environment quality of various cities fluctuated greatly with time, but the overall trend showed a slow downward trend, and there were obvious regional differences in coordination degree. The coordination degree between urbanization and economic development quality had been continuously improved, but the degree of coordination between urbanization and ecological environment quality, economic development and ecological environment quality had generally declined.

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Study on the quality measurement and spatial difference comparison of urbanization of prefecture level cities in Henan and Shaanxi provinces

JIA Yi-bin, WANG Han

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 99-105. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.020

Abstract （561）

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On the basis of the analysis of the quality of urbanization, from 4 aspects of the quality of economic development, the quality of social development, the quality of space environment and the quality of urban and rural integration, 19 indexes were selected to construct the index system of urbanization quality evaluation and conduct the comprehensive measurement and analysis of the urbanization of 29 cities in Henan province and Shaanxi province by entropy method. Then, clustering analysis was carried out according to the comprehensive score. The spatial difference of the results was compared, and some feasible suggestions were put forward, so as to enhance the quality of urbanization of metropolitan area and provide a theoretical basis for promoting the construction of “The Belt and Road”. The results showed that the average level of urbanization in Henan province was higher than that of Shaanxi province, but the development level of urbanization in Shaanxi province was more balanced than that of Henan province. The quality of economic development and social development in Henan province were less than that of Shaanxi province, but the quality of space development and urban and rural integration were stronger than that of Shaanxi province.

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Research on promoting poverty alleviation in the eco-tourism development of Shangluo city from the perspective of precision poverty alleviation

WANG Xin-xin, WANG Yi, GUO Meng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 106-108. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.021

Abstract （375）

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In recent years, relying on the advantages of high-quality air,vast forests, good water resources and other ecological resources, regional eco-tourism has developed rapidly in China, and has gradually become the first choice for people to carry out tourism activities. The development of eco-tourism resources can inject vitality into the local economic development, which also makes the development of eco-tourism an important way and an important measure to implement the precise poverty alleviation policy in China.Starting from the relationship between precise poverty alleviation and regional eco-tourism, this research studied the development status of Shangluo city ecotourism and drew on relevant literature to explore the development approach of Shangluo city ecotourism from the perspective of precise poverty alleviation, so as to stimulate Shangluo’s social and economic development, promote the implementation of precise poverty alleviation policies, and increase the effectiveness of poverty alleviation.

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The practice and enlightenment of coordinated promotion of poverty eradication and rural revitalization： Based on the survey in Shaanxi province

LI Jing-jing, DU Zhi-min

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 109-113. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.022

Abstract （372）

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Coordinated promotion of poverty eradication and rural revitalization are not only the inevitable requirements to solve the contradiction between the people’s growing need for a better life and the unbalanced and inadequate development, but also the inevitable requirement to achieve the goal of “two hundred years”. Based on the research and analysis of Shaanxi province, the practice of coordinated promotion of poverty eradication and rural revitalization was analyzed and summarized from the five aspects of industrial revitalization, talent revitalization, cultural revitalization, ecological revitalization and organization revitalization. Three inspirations were put forward： Establishing and perfecting the institutional mechanism was the institutional guarantee,adhering to perfecting the Party’s leadership of the work of agriculture, rural areas, and farmers was the political guarantee, and cultivating and stimulating the endogenous motivation of the poor was an important hand, for the coordinated promotion of poverty eradication and rural revitalization.

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Study on sustainable development after relocation of ex-situ poverty alleviation from the perspective of rural revitalization： A case study of Pingli county, Ankang city

ZHANG Ying

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 114-117. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.023

Abstract （389）

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Relocation of ex-situ proerty alleviation is one of the “five-one-batch” for poverty alleviation. At this stage, the focus of poverty alleviation work will shift from poverty eradication to stable poverty eradication and sustainable development. Rural revitalization is a major strategy for securing a decisive victory in building a moderately prosperous society in all respects and in socialist modernization. It can be said that the proposal of the rural revitalization strategy is to stand on a higher level to solve the problem of sustainable development of rural areas and farmers. There is an inherent consistency between the relocation of ex-situ poverty allaviation and the rural revitalization. In this study, Pingli county, Ankang city was selected as a sample to sum up the experience of sustainable development after relocation of ex-situ proerty alleviation in this region in terms of employment, industry, entrepreneurship and community management. The problems and obstacles of the sustainable development of the relocation of ex-situ proerty alleviation in the relocation area were analyzed, and some countermeasures connecting with the revitalization of the rural areas were put forward, so as to provide references for the theoretical and practical researchers of the relocation of ex-situ poverty alleviation.

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Research on the integration mode and path of agriculture and tourism in Northeast Sichuan Economic Zone

TAN Ji-cheng, CAI Wen-chun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 118-121. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.024

Abstract （306）

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Based on the study of the integration development of agriculture and tourism in Northeast Sichuan Economic Zone, four modes of integration development were summarized： City based, scenic area based, agricultural industry based and folk customs based. Combining with the problems existing in the integration development of regional agriculture and tourism, some countermeasures and suggestions were put forward： Improving infrastructure, enriching tourism elements of tourist attractions, improving the quality of practitioners and mobilizing various forces to join in the integration of agriculture and tourism.

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Analysis on the differential development path of community participation in rural tourism： Take Lihuaxi in Xinjin as an example

ZHENG Kun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 122-126. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.025

Abstract （455）

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Based on the literature review, the article sort out the differences in community participation in rural tourism in terms of perceived attitudes, forms of participation, and modes of participation. Taking Lihuaxi in Xinjin as an example, the development path of community participation differentiation in rural development of Lihuaxi rural tourism was analyzed. The following three conclusions were drawn： First, the development of rural tourism need to focus on the comprehensive benefits of economy, society and ecology; Second, community participation in rural tourism should not only concern the number of participants, but also the quality and ability of community participation; Third, high-quality ecology environment has always been an inexhaustible conclusion for the development of rural tourism.

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Research on influence of rural tourism development on rurality base on villager’s perception：Take of Longwan village as an example

LI Wen-rui, LI Zhen-ting

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 127-132. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.026

Abstract （435）

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Taking Longwan village in Jingtai county of Gansu province as an example, the connotation of rurality and its changes in the context of rural tourism were explored through designing the measurement scale, conducting questionnaire survey and interviewing. Three main conclusions were obtained as follows. Firstly, rurality consists of four measurement dimensions： Rural ecology, rural life, rural production and rural population. Secondly, from the perspective of villager’s perception,the correlation of rural ecology, rural life dimension and rurality was higher than the dimensions of rural production and rural population. Finally, The development of rural tourism had posed a significant positive effect on rural ecology, rural life, rural production and rural population. The development of tourism slow down the weakening process of the rurality to some extent; The rural tourism development had a significant positive impact on the local rurality.

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Study on the poverty alleviation path of tourism industry in contiguous poverty areas under the background of precision poverty alleviation： Taking southern Shaanxi as an example

LIU Long-long, FANG Yong-heng, ZHANG Hong-xia, CHENG Rui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 133-136. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.027

Abstract （390）

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Under the background of precision poverty alleviation, through the analysis of the current situation of poverty alleviation in the tourism industry in southern Shaanxi, some existing problems are found, such as the poverty-stricken people have weak awareness of tourism poverty alleviation, lack of tourism poverty alleviation professionals, lack of regional characteristics of tourism industry poverty alleviation, weak tourism market expansion, and rural tourism infrastructure conditions are weak. Combining with the actual development of poverty alleviation in the tourism industry in southern Shaanxi, it is proposed that the participation of endogenous driving should be promoted to enhance the participation of interest topics, the training and introduction of professional talents should be strengthened in the tourism industry, the market propaganda and innovation should be increased, and the “Internet + tourism poverty alleviation” model shoud be vigorously promoted, the development and adherence to the sustainable tourism industry poverty alleviation development principles and other path measures should be insisted, so as to to promote contiguous poverty-stricken areas in tourism poverty alleviation work to achieve a comprehensive victory.

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Research on rural collective economic organizations participating in tourism targeted poverty alleviation

ZHANG Man

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 137-139. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.028

Abstract （369）

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The rural collective economic organization was an important part of the national economy. It had the characteristics of mutual assistance and common prosperity, and has played an important role in promoting the development of the rural economy. This study focused on the related theoretical concepts of rural collective economic organizations and tourism targeted poverty alleviation, the problems existing in rural collective economic organizations participating in tourism targeted poverty alleviation were analyzed, and the corresponding countermeasures were proposed.

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Planning and research on rural study tourism products： Take Zhangwu village, Anji county of Zhejiang province as an example

ZHU Zhi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 140-143. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.029

Abstract （495）

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The current situation of tourism development and rural tourism resources in Zhangwu village, Anji county, Zhejiang province were studied and analyzed, and it was suggested that it should focus on creating study tourism products with the theme of Chang Shuo culture, Zhangwu Jinlong culture and fan culture. At the same time, it should integrate the rural culture, agriculture and tourism of Zhangwu village, extend the tourism industry chain, and finally form study tourism products of “researching Chang Shuo culture, pondering the ancient village of Zhangwu” and enhance the popularity of Zhangwu rural tourism.

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Coupling and development strategy of edible fungi ecological leisure tourism in southern Shaanxi

HU Ming-han

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 144-146. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.030

Abstract （338）

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Southern Shaanxi was rich in eco-tourism resources, and the growth conditions of edible fungi were superior. Through the coupling theory, this study analyzed the public coupling, content coupling and control coupling between edible fungus industry and eco-tourism industry in southern Shaanxi. The integrated development of edible fungus industry and eco-tourism industry in southern Shaanxi was an innovative business form of green agriculture and ecological revitalization, which embodied the core significance of harmonious development between human and nature. On this basis, this study put forward four linkage development strategies, including industrial organization, industrial design, brand publicity with industrial characteristics, research and development of new products and technology update.

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Think again about the protection of rural financial consumer rights and interests from the perspective of rural revitalization

SHAN Lin-bo

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 147-152. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.031

Abstract （361）

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The protection of rural financial consumers’ rights and interests is becoming more prominent and important along with the deepening of poverty alleviation and the advancing of rural revitalization strategy. This indicates that legislation is needed to endow rural financial consumers with new connotation, strengthen the rural financial consumer services and commodity supply, complete the system of information disclosure, consumer rights and interests protection of new rural financial products, and dispute resolution of diversified financial consumption, improve the effectiveness of rural financial consumer education, resolve the dilemma of rural financial consumer rights protection in a targeted way, and to promote financial support for the strategy of poverty alleviation and rural revitalization.

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Research on the innovative route of rural financial services under the strategy of rural revitalization

PAN Deng, ZHANG Bei-yu, ZHANG Yun-hui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 153-157. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.032

Abstract （486）

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Rural financial service revitalization is the inevitable requirement of returning the finance to its roots, supporting economic growth and realizing poverty reduction. Fully thinking through the profound change in developments of urban villages and taking more targeted measures to improve the rural financial service quality for revitalization are the inherent requirements of the modernization of rural industry. However, from the perspective of practice, there still exists many problems and challenges in revitalizing the economy with respect to rural financial services. As a consequence, it is necessary to choose the appropriate strategies and optimize the methods of rural financial service revitalization.

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Custom value of farmland and its measurement based on willingnesstopay：Taking Changliu and Lingshan towns in Haikou as an example

LI Sha, QI Rui, LIN Qiao-wen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 158-162. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.033

Abstract （283）

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The news of land-lost farmers cultivating the green space in the resettlement community is frequently seen in the newspapers, which has become a big problem of community governance. Based on this, this study put forward the conception of custom value of farmland. Using the double-bound dichotomous contingent value method to design a questionnaire, and taking land-expropriated peasantsin Haikou Changliu town and Lingshan town as samples, The methods of random utility model and multi-kind Logit model are applied in this paper to measure the custom value of farmland. The results show that the farmland has the function of carrying the peasants’ farming labor habit, and the value of this function is called custom value of farmland. The complete land expropriation will lead to the welfare loss of the value. Age and the emotion of farmland have significant positive influences on custom value; the educational level and occupation type have negative positive influences on custom value. 83.5% respondents have the willingness to pay. The average willingness to pay for custom value of the two towns is 51.62 yuan per household, with a total value of 7.99 million yuan. Hence, relevant departments in the process of land expropriation should not only pay attention to the compensation for the visible loss of land-lost peasants, but also take targeted measures to help them adapt to the sudden change of living habits quickly and effectively.

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Exploring the construction of rural culture under the background of rural revitalization

MIAO Li

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 163-165. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.034

Abstract （350）

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The 19th National Congress of the Communist Party of China （CPC） put forward the strategic goal of rural revitalization, which made the rural construction and development enter the white-hot stage. However, there are some problems in the concrete implementation of the strategic goal of rural revitalization at present, such as excessive pursuit of rural economic development while neglecting the construction of core rural culture. Compared to hardware construction, rural culture presents bigger loss, the current situation of rural culture, quality, and the state, development trend and country to promote the modernization needs also exists certain deviation, as a result, in this paper, the country revitalization under the background of rural culture construction are faced with the problem analysis and put forward suggestions for improvement, discusses the correct way of rural culture construction.

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Study on the dilemma and countermeasures of rural cultural governance in the context of rural hollowing

XU Jia-jun, SUN An-qi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 166-169. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.035

Abstract （465）

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Rural cultural governance as an important way to condense local spiritual civilization and optimize rural social system, it is an important part of the overall layout of rural revitalization strategy. However, under the current stage, the phenomenon of rural hollowing out is becoming more and more serious, which has brought some obstacles for rural cultural governance. This paper first describes the two basic characteristics of rural hollowing out, namely, industrial hollowing out and population hollowing out, discusses the positive significance of cultural governance in rural governance, and then analyzes the practical difficulties caused by rural hollowing out on cultural governance, such as the absence of the main body, the decline of local civilization and the limited development of cultural industry, and puts forward countermeasures and suggestions for rural cultural governance.

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Study on the protection and development of Tujia traditional villages in western Hubei under the strategy of rural revitalization：A case study of Pengjiazhai village in western Hubei

RAN Di-si, CHEN Lan, CHEN Yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 170-173. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.036

Abstract （344）

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The implementation of the new era of rural revitalization strategy aims to help change the imbalance between urban and rural development, realize the revival of local culture and the development of the country. This strategy brings new opportunities for the protection and development of traditional villages. Taking Pengjiazhai village as an example, guided by the strategy of rural revitalization, through the field investigation and analysis of Pengjiazhai, this paper summarizes the protection and development strategies of Pengjiazhai village, and constructs new development ideas for the traditional Tujia village in western Hubei.

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Research on the performance evaluation and countermeasures of the cultivation of new professional farmers

PAN Ze-jiang, ZHANG Yan-xiang, PAN Chang-jian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 174-177. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.037

Abstract （346）

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The cultivation of new professional farmers is an important breakthrough of promoting the organic connection between small farmers and the development of modern agriculture. In this study, DEA performance evaluation method was used to analyze the input-output efficiency of new professional farmers cultivation in seven pilot cities （counties） such as Yidu city. It has become the main direction of the policy reform and improvement of farmer cultivation policy in China to accelerate the cultivation support, improve the allocation of resource structure, and enhance the ability of scientific and technological innovation.

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Analysis on the difficulties and countermeasures of rural youth returning to entrepreneurship under the background of rural revitalization

TAN Can

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 178-182. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.038

Abstract （672）

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Under the background of rural revitalization, the traditional urban-rural population movement pattern has changed. Under the influence of the reverse thrust of urban integration barriers, the pulling force formed by of rural development and other related factors, returning home to start a business has become a rational choice based on economy and emotion, and has gradually developed into a new trend of population flow. However, the process of returning home to start a business has not been smooth sailing. Various practical factors make entrepreneurship in trouble. The qualitative research of young entrepreneurs returning home in the context of rural revitalization finds that entrepreneurial process of rural youth is full of thorns and challenges due to the dual influence of external factors and personal factors. Only when the government guides and supports them at the macro and micro levels, can we realize the healthy operation of the rural youth entrepreneurship system, and further promote the realization of rural revitalization.

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Study on citrus farmers’ adaptation to climate change based on SOR theory

YANG Jie, HOU Lin-chun, LI Hui-qin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 183-189. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.039

Abstract （480）

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Based on stimulus-organism-response （SOR） theory, the survey data of fruit growers in the citrus planting area of Wangjiaqiao in Zigui county were used to analyze the adaptive behavior mechanism of citrus fruit growers to climate change combined with structural equation. The results showed that adaptation of fruit growers to climate change was an adaptive measure after the assessment of perceived value and perceived risk. Fruit growers were acutely aware of climate change, and the perceived risk of fruit growers to temperature was 0.45. Perceived value had a positive effect on the adaptive behavior of fruit growers, while perceived risk had a negative effect on the adaptive behavior of fruit growers. From the micro perspectives of individual fruit growers, it is of great significance for the government to formulate appropriate policies and improve the adaptability of fruit growers to explore their adaptation behaviors to climate change.

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Research on the path of organic connection between small famers and modern agriculture in Qingdao city

FAN Cong-cong, SUN Yu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 190-194. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.040

Abstract （355）

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Taking Qingdao city as an example, based on the questionnaire survey of fruit and vegetable growers and the visit survey of main brands of fruits and vegetables, this study analyzed the main problems existing in the production and operation of fruits and vegetable products in Qingdao city. On this basis, the study discussed the path of connection small between farmers and modern agriculture in Qingdao city, constructed the modern production and marketing path of agricultural products in Qingdao city, so as to provide decision-making reference for realizing the organic connection between small farmers and modern agriculture, boost the development of agricultural economy in Qingdao city, and construct the agricultural modernization system with Chinese characteristics.

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Research on sustainable development of rural logistics based on GIS technology

LI Xin-le

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 195-198. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.041

Abstract （361）

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With the increasing penetration of e-commerce into many areas of rural society and economy, rural logistics is also gradually developing and improving. This study builds a rural logistics distribution system supported by GIS technology, optimizes the package transport path, monitors the transportation vehicles in real time, gives full play to the advantages of GIS technology, and realizes the whole processing management of parcel distribution, so as to ensure the sustainable development of rural logistics.

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Research on the realization mechanism of social responsibility of agricultural products processing industry based on game theory

LI Mao-hua, LIU Yan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 199-203. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.042

Abstract （423）

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Agriculture is an overall and strategic issue related to the economic and social development of a country, which is crucial to the prosperity and development of a country, the improvement of people’s lives, and the long-term stability of society. The realization of corporate social responsibility can prevent risks and promote the improvement of business operation, which is of strategic significance for the development of the whole industry. Firstly, this study introduces the background and current situation of the research, then constructs the realization of social responsibility of agricultural product processing industry by using the method of game theory, and makes a numerical simulation analysis with MATLAB R2019b software, and summarizes the solution to improve the realization mechanism of social responsibility of agricultural product processing industry based on game theory. It has a certain reference value for the better formulation of government guidance.

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Study on the mutual support of rural empty nesters from the perspective of policy network

YAN Yao, JI Nian-fang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 204-207. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.043

Abstract （351）

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Since the reform and opening up, a large number of young and middle-aged rural people have gone to cities for work, and there are more and more empty nesters in the countryside. In order to better solve the problem of rural empty nesters, various regions have started to explore the mode of mutual support for the aged. However, with the increasing number of empty nesters in rural areas, the mutual support in rural areas is in urgent need of improvement. Therefore, from the perspective of policy network theory, this study divides the participation in rural mutual support into five types in policy network theory. Based on the characteristics and division of labor of the five types in theory of policy network, this study discusses the role of each subject in the rural multual support, so as to promote the development of rural mutual support.

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The present situation and governance path of the rural population hollowing in China

XU Jia-jun, ZHANG Meng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 208-210. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.044

Abstract （1162）

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The rural population hollowing is the response of the great social and economic changes in China at the rural population level. Based on the current research on the rural population hollowing, this study reviews and collates the existing research from the aspects of concept definition, formation mechanism and temporal and spatial distribution of rural population hollowing by using the literature method, attempts to reveal the current situation of China's rural population hollowing and its impact, and then puts forward the corresponding governance paths from the three dimensions of agriculture, farmers and rural areas.

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The construction of performance evaluation system of rural public health governance based on BSC：A case study on the outbreak of COVID-19 epidemic in S town,Henan province

XU Peng-fan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 211-215. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.045

Abstract （354）

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Taking the COVID-19 epidemic situation in S town of Henan province as an example, the balanced score card （BSC） is used as a performance evaluation tool. This study analyzes the value from three aspects of responsibility orientation, effect orientation and demand orientation of epidemic prevention and control, makes a one-to-one replacement of financial indexes customer dimension, internal operation index, learning and growth level of the epidemic prevention efficiency, villagers’ identification and participation, clarifying epidemic prevention rights and responsibilities, ensuring the sustainability of epidemic prevention and balanced scorecard （BSC）. Taking the construction of public health management performance evaluation system as the breakthrough point of improving public health governance ability. The evaluation system of epidemic prevention performance of S town in Henan province was constructed from four indexes of epidemic prevention efficiency, customers （villagers）, internal operation, learning and growth. In order to promote COVID-19 in villages and towns better, and open up a new situation in rural public health management.

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Evaluation and improvement strategy of livable satisfaction degree of oasis city in arid area：Take Xinjiang as an example

JIN Xiao, DU Hong-ru, XIAO Feng-ling

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 216-221. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.046

Abstract （402）

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This study constructs the satisfaction evaluation index system from six aspects of urban characteristics, urban transportation, urban public facilities, urban public services, urban livable environment and urban public security, carries out questionnaire survey, selects typical cities（counties） in southern and northern Xinjiang for sample analysis, and uses factor analysis method to comprehensively evaluate the livable satisfaction of cities in Xinjiang. The results showed that the overall satisfaction of residents to urban livability was general, in which the residents’ satisfaction with urban livable environment and urban public security were higher, while that of urban infrastructure and urban public management were relatively low, and that of urban transportation was the lowest. Finally, the corresponding measures to improve the livability of the city are put forward.

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Study on the effect evaluation and optimization countermeasures of rural toilet renovation：Taking Pingquan city as an example

ZHAI Wen-zeng, WANG Cheng-wu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 222-227. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.047

Abstract （403）

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Taking Pingquan city as an example, based on the acceptance of the toilet renovation project in 2019 in Pingquan city. This study conducted a questionnaire survey among the staff of agricultural and rural bureau, township cadres, village cadres, technical workers in charge of toilet reform and villagers in Pingquan city. The fuzzy comprehensive evaluation method was adopted to evaluate the overall effect of the toilet reform in Pingquan city. In the process of evalution, the advantages and disadvantages of rural toilet reform were found, at the same time, the rectification countermeasures were put forword,which effectively promoted the transformation of rural toilets.

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The construction of rural environment collaborative governance system in the ecological development area of northern Guangdong

YANG Liang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (19): 228-232. DOI: 10.14088/j.cnki.issn0439-8114.2020.19.048

Abstract （336）

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As a strategic ecological development areas, northern Guangdong has great ecological value in the scope of province or even greater geographical space. According to the characteristics of rural areas in northern Guangdong and the current situation of environmental governance, this study constructs a collaborative governance system of rural environment in northern Guangdong province. The main contents include designing a systematic governance framework, building a government-society-market collaborative governance system and cross regional coordination system, and building a coordinated development of rural production-life-ecology.

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Research hotspots of rice brown planthopper [Nilaparvata lugens (Stål)]in China based on bibliometrics analysis

CHEN Yu-chong, FENG Rui, GUO Hui, CHEN Can, WEN Guo-quan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 5-9. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.001

Abstract （460）

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Research status of rice brown planthopper (BPH) in China was analyzed by the relevant articles in China National Knowledge Infrastructure (CNKI) database from 1958 to 2019 using the method of document metrological analysis.The results show that,①The number of published articles on BPH research in China has entered a period of substantial growth since 2004,with more than 100 articles published each year;②The key words that appeared with high frequency included rice varieties,wing morph,biotypes,and insecticides resistance;③In the past 10 years,it had frequently appeared in the BPH articlesfor RNA interference (RNAi) and molecular marker-assisted selection;④The most productive authorsare HUANG Fengkuan from the Institute of Plant Protection,Guangxi Academy of Agricultural Sciences,and FU Qiang from the China National Rice Research Institute,in the field of rice brown planthopper research in China,each published 94 BPH articles;⑤The main journals that publish papers in this research field are “Chinese Bulletin of Entomology”,“Acta Entomologica Sinica”,“Plant Protection”,“Journal of Zhejiang Agricultural Sciences”and “Chinese Journal of Rice Science”.There were more than 100 papers on the brown planthopper in each of these journals.With the promoting of green prevention and control crop diseases and insect pests,rice breeding for insect resistance has attracted the attention of researchers,especially with the method of molecular marker assisted selection has been widely used in rice breeding to improving the resistance of riceplants to BPH;RNAi is a powerful tool for insects functional genome research.It is also the first choice for new strategies of resistance in rice,and it has become a new research hotspot in the field of BPH research.

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Analysis on the application of pollen in health food

RUAN Wen-hui, WU Zhe, ZHANG Jiao, FENG Xia-zhen, YE Song-hua, LIU Guan-qun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 10-14. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.002

Abstract （434）

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With searched the special food information query platform of the State Administration for Market Regulation,it got the health food using pollen and related production enterprise information.Then it made a statistical analysis on the production province of health products,the year of listing,efficacy,dosage form,formula,registered capital of production enterprises and so on.So far,there were 175 kinds of domestic health products containing pollen,and there were 133 manufacturers,distributed in 23 provinces.There were some problems,such as lack types of pollen development,limited development power of pollen producing provinces,backward overall technical level of products,small scale of enterprises and so on,and some suggestions for targeted development had put forward.

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Research and implications of agricultural products traceability system from domestic and developed countries

CHEN Ping-ting, LUO Zhi-qing, GUAN Bo, MA Hai-rong, DENG Dan-dan, ZHANG Yue-ting, XU Gui-mei, SHEN Xiang-cheng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 15-20. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.003

Abstract （530）

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The agricultural product traceability system is to establish the whole process information base of agricultural prod uct supply chain through collecting data inthe production,processing,logistics and sales of agricultural products,and to protect and strengthen the traceability system of the quality safety management and risk control of agricultural products.The European Union,the United States,Japan and Australiahave many successful experiences in the construction of the agricultural product quality safety traceability system.This paper makes a careful study of the practices of developed countries from three aspects,the construction of laws and regulations,the construction of standard system and the application of traceability system.Combined with the present situation of small farmers' economic production in the primary stage of socialism in our country,we puts forward some relevant suggestions for realizing the agricultural product quality safety traceability system in our country.The suggestions are perfecting the traceability legislation,constructing coordinated agricultural product standards,andestablishing a national unified system platform by the government which carries out pilot implementation in stages.

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Comparative study on selenium enrichment ability of high quality rice varieties in Yichang city

DENG Hong-jun, ZHU De-ping, CAO Peng, ZHENG Shou-gui, LI Chun-hong, XU Yuan-chun, LIU Yun-fa

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 21-24. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.004

Abstract （994）

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In order to screen out high-quality rice varieties with strong selenium enrichment ability,the selenium enrichment ability of eight high-quality rice varieties planted in large area in Yichang city was studied by spraying different concentrations of organic selenium (4.5 L/hm²,7.5 L/hm²,12.0 L/hm²).The results showed that Quanyou 10,Xiliangyouchaozhan,Quanyou 534,Jingliangyou 534,Ezhong No.5 and Efengsimiao could be used as common selenium-enriched rice(The selenium content was 0.100~0.199 mg/kg) production varieties,Guxiang88 and Yuzhenxiang could be used as special selenium-enriched rice(The selenium content was 0.200~0.300 mg/kg) production varieties.

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Screening of high temperature tolerant rice (Oryza Sativa L.) varieties from current commercial varieties and materials

WANG Chun-hu, MU Qi-lin, WANG Song, TIAN Xiao-hai

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 25-28. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.005

Abstract （531）

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By simulating the daily temperature change pattern of typical local high temperature stress in a climate chamber,14 rice (Oryza sativa L.) varieties were treated with high temperature at flowering stage by cascade-temperature testing method.Assessing the fertility rate of varieties under different temperatures,the heat tolerance index (HTI) and integrated heat tolerance index (IHTI) of each variety were calculated,and the heat tolerance comprehensive index was used as the final index to determine the heat tolerance of rice varieties.The results showed that the comprehensive heat resistance index of Quanyouhuazhan and Quanliangyouhuazhan were greater than 2.5,and the heat resistance index was greater than 0.75 when the daily average temperature were 30.0 and 32.0℃,respectively.When the daily average temperature was 34.0,the heat resistance index decreased significantly.The comprehensive heat tolerance index of other varieties was lower than 2.5,and when the daily average temperature was 32.0,the heat resistance index was 0.51 or less.Two more tolerant varieties of Quanyouhuazhan and Quanliangyouhuazhan were selected,which can be used for extension in commercial production in greater area in the future safely.Suitable heat avoidance measures should be taken for those varieties with 2.0≤IHTI<2.5,and for varieties with IHTI<2.0,their commercial use should be limited to spread in areas where heat damage often occurs.

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Cultivation technique of machine transplanted rice under integrated rice-crayfish cultivation in Hubei province

CHENG Jian-ping, WANG Ben-fu, ZHANG Tao-juan, LI Yang, ZAHGN Zhi-sheng, YANG Xiao-long, PENG Cheng-lin, YANG Xiao-hong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 29-31. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.006

Abstract （411）

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In recent years,due to the low efficiency of rice planting in some areas of Hubei province,the phenomenon of “heavy shrimp over rice” and extensive and irregular rice planting methods and technologies have affected the healthy and sustainable development of rice and crayfish industry.Through many years of experiments and demonstration,this paper summarized the key points of rice machine transplanting cultivation technology in Hubei province,in order to improve the labor productivity and rice planting efficiency under the rice-crayfish cropping mode,and realize the balanced development of rice and crayfish double wheel drive.

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Characteristics of water-saving and drought-resistant rice and its high-yield and efficient cultivation techniques

TU Jun-ming, DENG Qiao-jiang, CAO Zhi-gang, CHEN Jie, JIN Hong-mei, ZHANG Qun, SONG Shu-yuan, YU Xin-qiao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 32-34. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.007

Abstract （417）

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Water-saving and drought-resistant rice refers to a new type of cultivated rice variety that combines high-yield and high-quality rice with drought-resistant and water-saving characteristics.In recent years,the development trend of drought and heat damage has become more serious,and the shortage of water resources has brought serious threats to rice production.The research on water saving and drought resistance has become one of the hotspots in rice research today.This paper analyzes the main advantages of planting water-saving and drought-resistant rice,and summarizes the main points of high-yielding and efficient cultivation techniques for water-saving and drought-resistant rice,with a view to providing a reference for the effective promotion and application of the technology.

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Genetic variation of SSIIIa gene in Yunnan special rice with high resistant starch

LI Xia, DU Juan, YANG Xiao-meng, PU Xiao-ying, YANG Jia-zhen, ZENG Ya-wen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 35-38. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.008

Abstract （375）

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The reported SNP splicing site of SSIIIa gene was detected on Yunnan special rice lines with high resistant starch and “Gongmi”series of varieties.The results showed that there was no reported splicing site SNP mutation in Yunnan high resistant starch materials.Furthermore,genetic variation analysis of SSIIIa gene sequence of Yunnan high-resistant starch special rice was conducted,and a total of 59 genetic variation sites of SNP were found,and 6 deletion/inserted mutation sites were detected.Among them,18 SNPs were distributed in the gene coding region,and 12 SNPs were non-synonymous variants.In addition,the differences of these non-homogenous variation loci between six high-resistant starch special rice materials and the local control varieties in Yunnan were analyzed.The results showed that the differences between six high-resistant starch special rice materials and the control varieties were mainly between indica and japonica rice,only Gongmi No.2 had snp-specific variation.

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Physiological and molecular differences of nitrogen response in wheat seedlings

XU Qing, XU Fu-chao, QIN Dan-dan, LIU Xiao-qiang, YUAN Xu, GE Shuang-tao, DONG Jing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 39-45. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.009

Abstract （449）

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To better understand the response mechanism of wheat seedlings under different nitrogen levels,a panel of 12 different wheat genotypes was set to grow under contrast nitrogen input levels by using the Hoagland nutrition solutions with sand.The seedling biomass,root morphology,chlorophyll content of the leaves,the leaves GS enzyme activity as well as the transcripts of the central genes involved in nitrogen uptake and assimilation process were investigated.The results indicated that the seedlings biomass of all the wheat genotypes was significantly increased upon the increase of the nitrogen input.The seedling root/shoot ratio under the low nitrogen level was higher than that under the high nitrogen levels.Although significant difference was observed for the main root growth between the different genotypes under the nitrogen-deficiency conditions,it was found that the nitrogen deficiency stimulated the main root growth and expanded the total root length and total root surface of the wheat seedlings on the whole.Meanwhile,the chlorophyll con-tent of the individual wheat lines were elevated upon the increase of the nitrogen input.However,the GS enzyme activity demonstrated genotypic variations under contrasting nitrogen levels.The gene expression of TaNRT2.1 and TaNRT1.1 exhibited higher transcript un-der low nitrogen conditions.Furthermore,the transcripts of TaNRT2.1 and TaNRT1.1 were higher in the high nitrogen use efficiency wheat lines of Zhengmai 9023 and“410187”than the low nitrogen use efficiency wheat line of Nanong 08Y611.In spite of up-regula-tion of TaGS2 was observed under high nitrogen levels,no significant differences were detected for the expression of TaGS1 with re-spect to the genotype or the nitrogen levels.

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Effects of EM bio-organic fertilizer on the production of microtubers from virus-free potato seedlings

ZHU Yuan-fang, XU Yong-qing, CHEN Fu-wei, FENG Yan-zhong, LIU Di, LI Feng-lan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 46-50. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.010

Abstract （363）

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The effects of EM bio-organic fertilizer on the agronomic traits,photosynthetic characteristics and yield of p otato microtuber were studied by using virus-free potato seedlings as experimental materials.The results showed that the survival rate and agronomic traits of potato seedlings were significantly improved by applying EM bio-organic fertilizer,and the yield of original seed was increased by 57.6% and 21.9% respectively by applying EM bio-organic fertilizer 1 and EM bio-organic fertilizer 2.

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Effect of returning rape straw to the field on soil nutrient supply

HUANG Yi-qiong, LIU Rong, ZHAO Chang-kun, SHI Liang, YANG Guo-tao, WANG Xue-chun, HU Yun-gao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 51-55. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.011

Abstract （389）

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In order to study the effect of rape straw returning to the field on soil nutrients under the condition of no tillage, different amount of rape straw returning to the field was set through pot and continuous field experiments to explore the effect of rape straw returning to the field on straw decomposition rate and nitrogen and phosphorus content in different soil layers under the condition of no tillage. The results showed that the amount of returning field had no significant effect on straw decomposition. The final straw decomposition rate was the highest when the whole amount was returned to the field. The highest nitrogen and phosphorus content was found in the tillage layer after the straw was returned to the field. The effect of continuous straw returning on nitrogen supplement of tillage layer was greater than that of phosphorus. Long term straw returning can reduce the content of nitrogen and phosphorus under the bottom of plough, reduce the loss of nutrient leakage, reduce the waste of resources and environmental pollution.

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Research on integrated model optimization of high efficiency and water-saving technology in Zhonggegen basin of Qitai county

WU Yu-xiu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 56-60. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.012

Abstract （398）

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Through the large-scale self-pressured drip irrigation technology in Zhonggegen basin in Qitai county,Xinjiang,conventional crop inputs and outputs under the three kinds of high efficiency and water-saving technologies of pond dam pressurized drip irrigation and automatic drip irrigation were investigated and the optimal analysis of benefit index system was carried out.The results showed that from the analysis of efficient water-saving technology,the comprehensive score of big-head self-pressured drip irrigation had the highest comprehensive score and the best comprehensive evaluation.While from the perspective of the comparison of crop planting types,hybrid maize had the highest comprehensive score.According to the comparison of technological preferences and the crop planting structure analysis,the use of the big head self-pressure drip irrigation and water-saving technology in the Zhonggegen basin to grow hybrid corn can increase the economic benefits of agricultural products and improve the farmers' production and income.

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Fertilization model and assessment of net greenhouse gas balance of summer maize in tidal soil area around Bohai sea

LIU Quan-feng, LIU Zhen-zhen, LAI De-qiang, SUN Yi, CHEN Xin, LI Ya-jing, WU Ting, MAO Cai-yun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 61-65. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.013

Abstract （296）

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The field plot experiment was carried out to establish fertilizer effect models of nitrogen,phosphorus and potassium fertilizer in different yielding fields in the tidal area around Bohai sea.The direct and indirect emission laws of greenhouse gases andthe net greenhouse gas emission balance were analyzed in the corn farmland ecosystem.The results show that the greenhouse gas emission intensity of the optimal economic fertilization amount of high,medium and low yield fields is smaller than the highest yield fertilization amount,the potential for warming is large,and it is a “sink”of greenhouse gases,which can be used as a scientific fertilization level for summer maize production in this area.

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Soil moisture variation rule and its relationship with climate factors in the dry land of the southern of Qinghai lake from 2001 to 2019

GUO Lian-yun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 66-70. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.014

Abstract （359）

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The data of soil moisture,air temperature and precipitation in dryland from 2001 to 2019 observed by Gonghe Meteorological Bureau of Qinghai province were analyzed by mathematical statistics,and upland 0~30 cm soil moisture variation characteristics in the Gonghe county over the southern of Qinghai lake was discussed,and a preliminary analysis was made to the climate impact factor.The results showed that soil moisture content increased significantly with the interannual extension in spring,but not significantly with the interannual extension in autumn.Soil moisture content in spring was higher than that in autumn,while the annual variation range in autumn was higher than that in spring.In spring,0~30 cm soil moisture content at all layers showed a significant increasing trend;In autumn,soil moisture content of 0~10 cm showed a slight increasing trend with the interannual extension;While soil moisture content of 10~20 cm and 20~30 cm showed a slight decreasing trend.In spring,the soil moisture content of 0~30 cm in each month showed a significant increase trend with the interannual variation,while the decreasing trend of soil moistur e content in September in autumn and the increasing trend of soil moisture content in October and November were not significant.Soil moisture content in May and October were significantly and positively correlated with the same month precipitation,soil moisture content in November was significantly and positively correlated with precipitation from July to September,and soil moisture in autumn was significantly and positively correlated with precipitation in September and autumn.

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Analysis of the influence of different underlying surface conditions on lightning characteristics in cities and plains based on ADTD

LI Zheng, FAN Min-qi, ZHAO Tao, WANG Xiao-fei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 71-74. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.015

Abstract （433）

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Using the lightning data in Hubei province meteorological department layout ADTD system to record the lightning location system laying,the difference of the surface features of lightning in the Jianghan Plain,Tianmen,Xiantao,Qianjiang three cities underlying surface and near the suburban plain are analyzed,in order to explain the influence of city and plain under different surface conditions on the characteristics of lightning.The results show thatIn the same atmospheric circulation background,the correlation coefficient of simultaneous lightning between urban and plain underlying surface is higher,because the proportion of local lightning generated by underlying surface characteristics is lower;The proportion of positive lightning of city underlying surface is higher than that of flash plain surface,but the average amplitude of positive lightning is low,inversely related;City underlying surface lightning amplitude distribution is more concentrated,mainly in 20~35 kA;Compared with the plain underlying surface,the average amplitude of lightning current is 0.91 kA higher,the median cumulative probability of lightning current is 0.90 kA higher,and the urban underlying surface of lightning current over 60.0 kA is also more.

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Evaluation of tourism climate comfort in Guidong county during 1981—2010

DENG Ling, LUO Xiao, LI Xue-min, JIANG Di-fei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 75-78. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.016

Abstract （509）

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Based on the basic climate data of Guidong county from 1981 to 2010,the weighs was graded by fuzzy analytic hierarchy process (FAHP) to build a comprehensive evaluation model of climate comfort,using the temperature and humidity index,wind efficiency index,clothing index and human comfort index.This model was used to evaluate the climate comfort of Guidong county in each month.The results showed that the comprehensive evaluation index of climate comfort in Guidong county was 4.42~9.00 during the year,the evaluation result of climate comfort index was less comfortable only in January over all the months,and the comfortable period (more comfortable,comfortable and extremely comfortable) was as long as 11 months.The most suitable months to travel in Guidong county were from April to June and from September to October,and the evaluation results of climate comfort index in these months were extremely comfortable;It was suitable to travle to Guidong county to avoid sunstroke from July to August,and the evaluation result of climate comfort index was comfortable.The evaluation results of climate comfort index in Guidong county were more comfortable from November to December and from February to March,and it was more suitable for tourism in these months.

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Construction of science and technology demonstration park for soil and water conservation in West Lake basin of Wuhan city

HUANG Xue-ying, WU Man, LI Bo-run, KE Hui-yan, DING Shu-wen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 79-82. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.017

Abstract （407）

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In order to carry out the national ecological civilization construction strategy,implement the “five development concepts”,and improve the scientific and technological level of soil and water conservation,the Bureau of Water Affairs and Lakes in Caidian District of Wuhan founded the establishment of science and technology demonstration park for soil and water conservation in West Lake basin of Wuhan city.In accordance with the principle of unified planning according to local conditions,using abandoned quarry and soil extraction site to build into a comprehensive science and technology demonstration park for soil and water conservation with five functional areas,including comprehensive management,scientific research and experiment,science popularization and education,ecological restoration,tourism service.The park has achieved remarkable results in scientific research demonstration,ecological industrialization demonstration and science popularization.This study summarizes the general situation,construction contents and achievements of science and technology demonstration park for soil and water conservation in West Lake basin of Wuhan city,and puts forward some relevant development suggestions.

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Species identification of Chironomidae in Leigongshan Natural Reserve

YU Hai-jun, WANG Yi-ting, HUANG Xin, TAI Chun-qing, ZHENG Hui-fang, WANG Qian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 83-87. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.018

Abstract （515）

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Based on DNA barcoding and morphology,species identification of chironomids in Leigongshan Natural Reserve was carried out.The results showed Leigongshan Natural Reserve,where contained 4 subfamily 12 genus 18 species chironomid,with the character of 12 genus were redescribed and attached the overall picture of 18 species.According to DNA barcodes analysis that gave 18 molecular operational taxonomic units,and keep conformed to morphological identification.

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Effects of 24.1% trifloxystrobin·isomitamide seed treatment suspension on rice disease control and yield

ZHANG Shu, HUANG Jia-xiang, LYU Liang, CHANG Xiang-qian, YANG Xiao-lin, WANG Zuo-qian, LI Chun-qing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 88-90. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.019

Abstract （736）

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The effects of 24.1% trifloxystrobin·isomitamide seed treatment suspension (FS) on rice disease control and yield of rice were studied.The results showed that the control effect of 24.1% trifloxystrobin·isomitamide FS for seed treatment were 100% on rice seeding blight and rice seeding blast,and the control effect of rice leaf plague was 86.2%~94.5%,which can also promote the growth of rice roots and increase the tiller number and effective panicles,and improve seed setting rate in the meanwhile.Compared with CK,rice yield increased by 2 157.0~4 335.0 kg/hm².The control should be carried out under condition that the environment and climate were favorable to leaf plague and panicle neck blast,such as planting rice blast varieties and large amount of nitrogen fertilizer,should be controlled,especially after the jointing stage of rice.It was recommended to use 24.1% trifloxystrobin·isomidamide FS 20~25 mL with an addition of 2 mL of film forming agent coating for dried rice seeds per kilogram.

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Disease survey of walnut fruit and fungal pathogen identification in Yunnan province

ZHANG Zhi-xiao, JI Mei, HU Lian-rong, LIU Ling

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 91-95. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.020

Abstract （377）

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A disease investigation of walnut fruit was carried out in 10 major walnut producing areas in Yunnan province,and the collected fruit specimens were sliced for observation and identification.The results showed that diseases were more common in Dali city and Chuxiong city;The main diseases were anthrax,fruit rot,fusarium wilt of fruit and melasma;Pathogen of fruit belonged to 17 species in 17 genera,among them Colletotrichum gloeosporioides,Fusicoccum sp.,Botryosphaeria dothidea and Pestalotiopsis disseminata had the highest occurrence rate.

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A Meta-analysis of Paris polyphylla seeds germination by different experiment ways

FAN Hua, LI Sen, XI Huan, TU Wei-guo, GOU Xiao-lin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 96-101. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.021

Abstract （340）

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By using the method of Meta-analysis,24 papers on seed germination of Paris polyphylla from 1990 to 2019 were selected from Chinese and English databases,and 78 independent experimental groups were obtained.The effects of temperature,machinery,auxin and compound treatment on seed germination of Paris polyphylla were analyzed,and the possibility of seed breeding of Paris polyphylla at higher altitude was discussed.The results showed the seeds germination of Paris polyphylla under constant temperature was a simple and reliable way,interactive treatments of constant temperatures,variable temperatures and sand storage increased seeds germination rate (relative germination rate was 0.61) greatly. Improper use of auxin will inhibit the seed germination of Paris polyphylla. Paris polyphylla Sm. var. yunnanensis (Franch.) Hand.-Mazz had higher germination rate and better stability of germination than Paris polyphylla Sm. var. chinese (Franch.) H. Hara under the same treatment conditions. There was little research about seeds germination of Paris polyphylla in higher elevation. It is necessary to study the breeding technology of Paris polyphylla at high altitude.

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Effects of different planting density on morphology index and yield of Premna microphylla Turcz.

QU Hong-jie, WANG Hui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 102-104. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.022

Abstract （424）

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To explore the effects of different planting density on morphology index and yield of Premna microphylla Turcz.,selected fourgermchitscoming from Anhui province,Henan province,Enshi Tujia and Miao Autonomous Prefecture,Baokang county of Hubei province,carrying on 3 kinds of different density cultivation experiment (The plant row spacing were 20cm×80cm,30cm×80cm,40cm×100cm).The results showed that a significant difference in yield and pectin content of Premna microphylla Turcz.,the yield of fresh leaf coming from Enshi Tujia and Miao Autonomous Prefecture tree was highest,the average yield of fresh leaf of each tree was 2500g every year.The pectin content coming from Anhui province tree was highest,the average pectin content per kilogram of leaves was 35.25%.Different planting density had obvious effect on the yield.The plant row spacing of 30cm×80cm was the best for the growth of Premna microphylla Turcz.The average annual fresh leaf yield per plant was 2269g.

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Research on the effects of intelligent sprinkler irrigation on reducing salt return in garden green space

CHEN Qing-bin, AN Lin, WEI Li-ying

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 105-107. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.023

Abstract （393）

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Taking Tianjin Gangcheng road garden green soil as the research object,the effects of intelligent sprinkler irrigation on salt return in saline-alkali soils under different irrigation conditions were studied,the suggestions to inhibit soil salinity in garden green land were put forward.The results showed that,using intelligent sprinkler irrigation under the condition of 2.7 mm/d irrigation,the water-soluble salt content of 0~20 cm showed a downward trend overall,the salt reduction rate was 16.1%,and it could well inhibit the return of salt in topsoil.At the same time,the effect of water saving was the best,and the cost of watering was the lowest.

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Study on leaf anatomical structure and photosynthetic characteristics of two bamboo species in spring greenhouse

ZHANG Jing-run, WANG Bin-cun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 108-111. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.024

Abstract （448）

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The paraffin sections of B ambusa ventricosa kimmeiand Bambusa ventricosa McClure were prepared by paraffin embedding method and their structures were observed by microscopy.The photosynthetic characteristics and the optical response parameters of two kinds of bamboo plants were measured by CIRAS-3 photosynthesis measuring instrument.The results showed that there were differences in anatomical structure and photosynthetic characteristics of Bambusa ventricosa kimmei and Bambusa ventricosa McClure.The thickness of the upper,lower epidermis,palisade tissue and sponge tissue of the leaves of Bambusa ventricosa McClure were larger than that of Bambusa ventricosa kimmei.After the comparison of five photosynthetic characteristics,the net photosynthetic rate,stomatal conductance and water use efficiency of Bambusa ventricosa McClure were stronger than that of Bambusa ventricosa kimmei,but the transpiration rate of the former was lower than that of the latter.From the light response curves of the two bamboo plants,it was found that the utilization of the Bambusa ventricosa kimmei was not as good as that of the weak light,and the photosynthesis was more susceptible to inhibition in the high light environment.

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Discovery of stomatal groups in six species of Rhododendron in Jiaozishan

BAI Rui-zhu, SU Yuan, ZHAO Chang-you, YIN Gen-shen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 112-115. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.025

Abstract （487）

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The leaf epidermal micromorphological pattern of six species of Rhododendron plants distributed in the Jiaozishan National Protected Area in Yunnan province were observed by the imprinting method.The leaf epidermal morphological index was measured in the software IT 2.00.The results showed that the stomata were distributed in groups in the six Rhododendron plants;and the significance test analyses exhibited that the average distance between the stomata within the stomatal group was significantly smaller than that between adjacent two stomatal groups.This phenomenon of stomatal groups is firstly found on the leaves of Rhododendron plants,and the physiological effects and formation mechanisms of stomata groups have been discussed.

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Landscape pattern analysis and landscape ecological planning of Xiong'an Baiyangdian Wetland

SHI Ying-jie, WANG Gui-xia, AN Guang-yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 116-123. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.026

Abstract （449）

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Landscape changes and its drivers of Xiong'an Baiyangdian wetland between 1999 and 2017 were analyzed based on remote sensing(RS) and geographical information system(GIS).Results showed that,from 1999 to 2017,wetland area showed a trend of rapid reduction first and then slow increase,with a decrease of 5 019.66 hm²,down by 19.35% totally;cultivated land and bare land area increased first and then decreased by a small margin;forest land and construction area increased year by year.The most prominent changes during 1999—2017 are the mutual transformation of farmland and bare land,and the mutual transformation of natural water,artificial water and aquatic plants.In the first and middle period,the outflow of wetlands was the main trend.From 2011 to 2017,the agricultural expansion was controlled and gradually transformed into the inflow of farmland and bare land to wetland.From 1999 to 2017,The number of patches in Baiyangdian wetland increased from 11 722 to 16 720,and the patch density increased from 36.08 to 51.47.The landscape fragmentation degree of Baiyangdian wetland increased.The Area-Weighted-Mean Shape Index showed a trend of decreasing first and then increasing,and the patch shape of wetland became more and more complex.With the increase of Shannon diversity index and Shannon evenness index,the landuse of Baiyangdian wetland is more and more abundant,and the dominant role of dominant patches is gradually reduced.The main reasons for the change of landscape pattern in Baiyangdian in the past 18 years are climate change,decrease of precipitation,economic development,expansion of urban and agricultural land,the government's policy of diverting water and returning farmland to lake,etc.Aiming at wetland restoration and sustainable development,Baiyangdian wetland is divided into six ecological functional areas.

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Preliminary study on the dwarfing effects of spraying four plant growth retardants on potted Tanacetum cinerariifolium

MAO Jing, TONG Jun, DONG Yan-fang, XU Dong-yun, FANG Lin-chuan, ZHOU Yuan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 124-126. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.027

Abstract （491）

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In order to control plant height of pyrethrum (Tanacetum cinerariifolium),B₉,CCC,PP₃₃₃,S₃₃₀₇ with different concentration levels were applied and compared by foliar sprays on the potted plant.Results showed that all the four plant growth retardants had dwarfing effects on plant height with different extent as well as on inhibiting plant crown width.The dwarfing effects and negative effects on plant growth were S₃₃₀₇,PP₃₃₃,CCC,B₉ from strong to weak.The optimum dwarfing treatment for pyrethrum was the application of 200mg/L B9.It not only reduced the height of potted plants to about 30% of that of the control plants,but also inhibited the leaf development and plant width of potted plants,the width of the plant was about 70% of the control plant,and the treated plant was short,compact and suitable for pot culture.

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Study on artificial propagation technology of Mystus guttatus

TAN Dong-chu, LIU Qing-shan, XU Hua-jian, WEI Xue-yao, HE Neng-zhen, WEI Xiang, HE Xing-heng, TAN De-qing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 127-129. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.028

Abstract （634）

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In 2019,the fish proliferation and discharge station of Qiaogong hydropower station used DOM,LRH-A2 and HCG for artificial oxytocin.Results showed that 4 batches of oxytocin were successful,with the 2-needle injection method.The average yield was about 57.5%,and the average fertilization rate was about 48.7%,and the average hatching rate was about 27.6%.The breeding peak was in June.The hatching of fertilized eggs was more affected by water temperature.The hatching effect was better between 26~28.The filming time was 62~64 h.The spot diameter of spotted eggs was 3.1~3.8 mm.The full length of the outcrop was 5~6 mm,and the length of the flat fry was 13.5~15.5 mm.

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Artificial cultivation of albino-Chinese pound turtles

REN Zhen-ming, REN Shao-ming, REN Ke-ming, LIU Bing-jing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 130-133. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.029

Abstract （1918）

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A wild male albino-Chinese pond turtle (P) was used to cultivate artificial albino Chinese pond turtle individuals.The albino-male turtle(P) was mated with ordinary female turtles to breed F₁ offsprings,The mature F₁ offspring female turtles were mated with the albino-male turtle(P) to breed F₂ offsprings.After 7~8 years ocultivation,the F₃ offsprings were cultivated albino-Chinese pound turtle individuals in 2008.This was the first time cultivated albino-Chinese pound turtles successfully.After the cultivation of the 5th generation(F₅) and 14 batches,the rate of emergence of albino-Chinese pound turtles have reached more than 80%,the stable gene with albino-Chinese pound turtles was developed for the first time.

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Comparison of silage yield and quality among twenty maize varieties(lines) in southwest China

JIAO Jin-long, ZHANG Yan-ru, WANG Wei, ZHAO Li, WU Yuan-qi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 134-137. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.030

Abstract （629）

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To compare the agronomic traits and productivity of twenty silage maize varieties(lines),so as to screen out the best silage varieties suitable for spreading cultivation in southwest China.The characteristics,silage yield and quality of different cultivars were analyzed under planting density of 4 266plants/667m².The results showed that there was a difference between the agronomic traits and the yield traits among different varieties.The highest yield of silage was 5 463.66kg/667m²,and the lowest yield was 3680.83kg/667m².Nutritional quality analysis of the six highest silage varieties(lines) (Yunnan Local Species/Pacific 98,chuanqing 8,76/77,1123A/different-7,H1619052/Pacific 98,9yco33/Yunnan Local Species) found,except for In Vitro Dry Matter Digestibility,the contents of crude protein,neutral detergent fiber,acid detergent fiber and soluble sugar were significantly different.Yunnan local species/Pacific 98 and Chuanqing 8 silage have high yield,good quality and excellent comprehensive traits,so they were suitable for popularization and planting in southwest China.

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Association analysis of polymorphism of pig DKK2 gene and sow litter size traits

ZHOU Jia-wei, QIAO Mu, WU Jun-jing, WANG Xiao-zhong, LIU Gui-sheng, MEI Shu-qi, PENG Xian-wen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 138-140. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.031

Abstract （339）

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The reproductive trait of sows is one of the important economic traits of pigs,but due to its low heritability,the conventional selection of reproductive traits of sows has been slow.SANGER sequencing was used to detect the SNP locus in the DKK2,and the correlation analysis was performed with the litter size traits.The results showed that there was a new SNP locus in the second intron of the DKK2,which was named 8:g.114967878A>G,and the SNP locus was significantly related to the number of live births.The new SNP site 8:g.114967878A>G of DKK2 in pigs has effect on the number of live births.

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Optimization of extraction process and determination of total flavonoids in leaves of Podocarpus macrophyllus (Thunb.) D. Don from different origin

LIU Rui, MA Jia-bao, LU Zheng-lin, WEI Jiang-cun, CHEN Chao, WEI Li-fu, CUI Jian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 141-144. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.032

Abstract （381）

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The reflux extraction method was used to extract total flavonoids from the leaves of Podocarpus macrophyllus (Thunb.) D.Don.The effects of extraction method,solvent concentration,extraction volume and extraction time on the content of total flavonoids in Podocarpus macrophyllus (Thunb.) D.Don were investigated by single factor test.The orthogonal test was used to optimize the extraction conditions and the optimum extraction process was selected.The results showed that the optimum extraction process of total flavonoids from the leaves of Podocarpus macrophyllus (Thunb.) D.Don was 75% ethanol,the ratio of material to liquid was 1:30(g:mL),and the reflux extraction time was 0.5 h.The method has good repeatability,stability and feasibility.

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Study on selenium leaching rate and influencing factors of selenium-enriched green tea in bag foam

ZHOU Qiong, PU Na

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 145-149. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.033

Abstract （447）

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The content of selenium is determined by hydrogen generation atomic fluorescence spectrometry with Ziyang selenium-enriched green tea in bag foam as raw material,and the leaching rate of selenium in tea is studied.On the basis of single factor,the conditions for improving the selenium leaching rate of bag selenium-enriched green tea are optimized.The results showed that the leaching rate increases with the increase of extraction time,but decreased with the increase of extraction time and ratio of material to liquid,and the leaching rate was obviously affected by the extraction times,extraction temperature,time and ratio of material to liquid.The orthogonal test and its verification test showed that the leaching rate of Ziyang selenium-rich green tea in bag foam could reach 28.64% under the conditions of extraction temperature 95,time was 6 min,ratio of material to liquid(g:mL) was 1:30 and extraction times was once.

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Optimization for purifying technologyof the leaves and steams of Panax notoginseng saponins extract with ZTC1+1 II clarifier by response surface method

QIN Feng, LI Jin-gui, ZHU Shan-yuan, WANG Hong-yun, WANG An-ping, WU Shuang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 150-154. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.034

Abstract （359）

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In order to determine the ZTC1+1 II type natural clarifying agent used for notoginseng (Panax notoginseng) leaves and steams glycosides extract the best technology of removing impurity,on the basis of single factor analysis,the concentration of drug quality,clarifying agent dosage,temperature is the independent variable,saponin content and solids removal rate as evaluation index,establishing three factors and three levels of Design- Expert response surface analysis method.The results show that the best process of Panax notoginseng leaves and steamspurificationis as followsThe mass concentration of liquid is0.19 g/mL,the order of ZTC1+1 II natural clarifier is first part A and after part B,the dosage of clarifiers B and A are 10% and 5% volume of the extract,respectively,and acting temperature is 40.Under this technological condition,the removal process of stem and leaf of Panax notoginseng is optimized,and the solid removal rate is 28.40%,the saponin retention rate is 92.22%,and the RSD is 1.74% and 0.56%,respectively.The repeatability is good,which verified the validity of the model.

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Design and implementation of meteorological service intranet platform in Hunan province

ZHONG Yan-wen, YIN Xin-huai, ZHU Liang, FENG Xian, XIA Zheng-long

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 155-158. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.035

Abstract （370）

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In order to realize the intensive integration of business system and provide one-stop online data access and sharing services,Hunan meteorological service intranet platform was established.The platform was based on the general J2EE application architecture;unified data source;distributed architecture and big data technology to improve the speed of query and statistics.The platform was based on the WebGIS framework of JavaScript,and realized the overlay of map layers by customizing various components.The platform provided unified business data,products and information services for provincial and municipal users,and provided effective supplementary means and data support for meteorological forecasting,meteorological observation,management and other business.

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Design and implementation of the support system of modern agrometeorological service in Shandong province

LI Yu-hua, CHU Xi, XU Cai-yan, LI Wen-ke, LI Xu-xu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 159-163. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.036

Abstract （430）

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In order to meet the needs of agrometeorological service and improve the capability of modern agrometeorological service,the support system of modern agrometeorological service in Shandong province was developed.By using LBS,data encryption synchronization,Web API release interface and HTML5 technology,the meteorological service data automatic collection module,meteorological service information data maintenance and management module,meteorological service information data release interface,meteorological service data application interface,user and API management module,meteorological service information monitoring module were designed.Based on API,the platform of agricultural meteorological service data release has been established,which can manage meteorological application services and data interface resources in a unified way,and provide strong support for the development of agricultural intelligent meteorological service terminals.The level of agrometeorological service has been effectively improved by the support system of modern agrometeorological service in Shandong province.

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Research on the input behavior of grain farmers in Dancheng county

SHEN Yu-jie

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 164-169. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.037

Abstract （376）

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Under the background of national food security,this research studied the input behavior of grain farmers in Dancheng county of Henan province from four aspectsland input,labor input,means of production input,and technology input.The results showed that,there were some problems in the input behavior of grain farmers in Dancheng county,such as limited land input,reduced labor force,excessive input of means of production,and low technical level.Some suggestions were put forward,such as developing moderate scale operation,cultivating new management subjects,increasing technical training for farmers ,and implementing soil testing and formula fertilization.

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Investigation on the tourist satisfaction of Tianzhushan national forest park

WANG Xin-xin, WANG Yi, GUO Meng, ZHAO Cui-cui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 170-172. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.038

Abstract （409）

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Tianzhushan national forest park located in Shangluo city of Shaanxi province,which was rich in ecotourism resources,but there were still many problems in the tourist satisfaction.This study clarified the concept of tourist satisfaction and other related concepts,collected data through questionnaire survey,used IPA quadrant analysis method to analyze the existing problems of the tourist satisfaction in Tianzhushan national forest park,in order to provide direction for taking targeted measures,so as to effectively improve the tourist satisfaction of Tianzhushan national forest park and promote the better and faster development of the scenic spot.

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Research on the promotion of new science and technology to agricultural economy in Shaanxi province:Take AI bionic cluster control system as an example

PENG Bo, CHEN Wei-feng, SHE Ke

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 173-176. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.039

Abstract （555）

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There were some problems in the development of agricultural economy in Shaanxi province,such as the low degree of intensification,the low per capita share of grain,the lack of agricultural capital investment and the imperfection of agricultural industry chain.In order to change the current situation,taking AI bionic cluster control system as an example,this study researched on the promotion of new science and technology to agricultural economy in Shaanxi province,focused on the selection of intelligence agriculture to promote the development of agricultural economy in Shaanxi province,expound the current situation of agricultural development in Shaanxi province,analyzed the problems existing in the development of agricultural economy in Shaanxi province,and finally put forward the measures to promote the high-quality growth of agricultural economy in Shaanxi province,so as to promote the social stability and harmonious development of Shaanxi province.

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Evaluation of land use intensity of universities in Chenggong district of Kunming city

LI Zhi-ying, WANG Lin, LONG Ye, CHEN Ning-xin, LI Chen-chen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 177-182. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.040

Abstract （369）

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The rough land use in colleges and universities not only wastes land resources,but also adversely affects the work and study of teachers and students.With reference to the evaluation system and evaluation model of land intensive use,taking the intensive use of land in 9 colleges and universities in Chenggong district of kunming City as the research object,the multi factor evaluation method was adopted to evaluate the land use degree,land use intensity,land use structure,input and output intensity of 9 colleges and universities.The results showed that Yunnan Jiaotong Vocational and Technical College,Kunming Medical University and Yunnan Arts College were highly intensively utilized;Yunnan University of Traditional Chinese Medicine,Yunnan Nationalities University and Yunnan Normal University were high intensively utilized;Kunming University of Science and Technology,Yunnan Open University and Yunnan University were general intensively utilized.Finally,relevant suggestions were put forward for the intensive use of land in colleges and universities.

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Research on the development of the universities' retired aging talent resources based on AHP-SWOT analysis method

JI Nian-fang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 183-187. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.041

Abstract （589）

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The retired aging talent resources in universities were an important part of the intellectual resources in China today.Combined with AHP method and SWOT method ,using AHP-SWOT analysis method,this study systematically analyzed the key influencing factors of the development of retired aging talent resources in universities,and calculated the weight of each factor.The results showed that,the development of retired aging talent resources in universities must focus on people-oriented,from the perspective of policy,system and platform to achieve sustainable development.

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Study on salary design of nursing staff in rural “combination of medical and nursing care” nursing homes in J province: From the perspective of ADL scale and standardized value model

ZHOU Xiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 188-191. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.042

Abstract （400）

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With the increasing demand of rural elderly groups for pension institutions,the demand for nursing staff in pension institutions was also gradually increasing.In order to stabilize the nursing staff and attract talents into the industry,this study analyzed and summarized the main problems existing in the salary design of nursing staff in rural “combination of medical and nursing care”nursing homes in J province.Based on the survey results,the ADL scale and the standardized value model were introduced to improve the salary design of nursing staff in rural “combination of medical and nursing care”nursing homes in J province.Through the model,the work contribution of nursing staff was quantified,and the explicit labor value and implicit labor cost of nursing staff were comprehensively considered.Then the standardized service value of nursing staff was calculated,and the salary was calculated based on the calculation results pay distribution,in order to provide new ideas for the salary design of nursing staff in rural “combination of medical and nursing care”nursing homes in J province.

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Economic development quality evaluation of Han River Ecological Economic Belt

YANG Ying-juan, GUO Lei-lei, GAO Yan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 192-196. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.043

Abstract （384）

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Han River Ecological Economic Belt covered some cities and counties in Hubei,Henan and Shaanxi provinces,and was an important part of the Yangtze River Economic Belt.However,in the actual development process of Han River Ecological Economic Belt,there were some problems,such as obvious regional differences,unbalanced industrial development and serious ecological damage,which seriously restrict the socio-economic development of Han River Ecological Economic Belt.Therefore,on the basis of constructing an evaluation index system of economic development quality,which included four dimensions of economic development effectiveness,economic development innovation,economic development sharing,economic development sustainability with the relevant index data of 14 cities in Han River Ecological Economic Belt from 2010 to 2017,the weight of each index was determined by principal component analysis,and the horizontal comparison and vertical comparison were made.The results showed that although the quality of economic development in the Han River Ecological Economic Belt was increasing year by year,but the innovative and sharing growth of economic development were slow,and the sustainability of economic development showed a downward trend in the later period.Finally,specific suggestions were put forward to improve the economic development quality of Han River Ecological Economic Belt.

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Difficulties, countermeasures of collaborative governance in the utilization of straw resources:Take Jingzhou city as an example

DUAN Ya-min, HUANG Qian-qian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 197-199. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.044

Abstract （403）

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The development of straw resources utilization has been paid attention by the country and society,but the development speed was slowly.This study analyzed the dilemma of collaborative governance of straw resources utilization in Jingzhou city,Hubei province.It was found that the concept of multi-agent collaborative governance was not strong,the function of multi-agent was ineffec-tive,and the operation of multi-agent collaborative governance was not coordinated,which affected the collaborative governance effect of straw resources utilization.On this basis,this study further put forward some countermeasures,such as strengthening the concept of multi-agent collaborative governance,improving the operation guarantee mechanism of multi-agent collaborative governance,and strengthening the communication among multi-agent,so as to improve the effect of collaborative governance,promote the develop-ment of straw resources utilization,and realize the construction of rural ecological civilization.

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Research on coupling and coordination between energy policy and green innovation of energy industry

ZHANG Ai-qin, XUE Bi-wei, GUO Pi-bin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 200-204. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.045

Abstract （324）

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Based on the data of energy industry from 2009 to 2015,this study used the grey correlation degree to verify the strong correlation of the selected indicators,and constructed the coupling and coordination model to empirically test the coupling and coordination between China's energy policy and green innovation of energy industry.The results showed that,the energy policy and the green innovation of energy industry changed from the industry development lag type to the policy development lag type in 2013,and the imperfect energy policy system restrict the green innovation growth of energy industry to a certain extent;the coupling degree between the two was always in the antagonistic stage,and there was still a big gap from the benign resonance development;the coordination degree between the two gradually was raised to the initial stage,but the rising speed was slow.The ability of energy policy to drive green innovation of energy industry need to be improved.The research was helpful to provide reference for energy management departments to improve the green innovation efficiency of energy industry and accelerate the transformation and development of regional economy.

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The influence of Haikou traffic on tourism industry development

ZHAO Lin-long, TIAN Yu-su

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 205-208. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.046

Abstract （384）

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Using Eviews 10.0 software,this study make correlation test,stationarity test and cointegration analysis between traffic and tourism revenue in Haikou city,and discussed the influence of traffic on tourism industry development in Haikou city.The results showed that,the traffic in Haikou city had a significant relationship with and promoted the tourism industry development,and the highway traffic in Haikou city had a supporting role in the tourism industry development,and put forward the corresponding development suggestionsexpanding domestic and international air routes,fully promoting the construction of Baohai high-speed railway,and actively constructing cruise tourism experimental area,so as to comprehensively promote the tourism industry development of Haikou city.

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Evaluation of English guided tour information service functions in ecotourism scenic spots: Take Wuhan East Lake Moshan scenic spot as an example

XUE Ju-hua, XIAO Yong-jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 209-214. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.047

Abstract （390）

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English guided tour information was an important part of scenic spot service.Its service functions should be comprehensively evaluated combined with the importance of information and the tourists satisfaction.Taking Wuhan East Lake Moshan scenic spot as an example,this study measured the objective perception degree of English guided tour information in scenic spots by combining analytic hierarchy process (AHP) and fuzzy comprehensive evaluation.The results showed that the guided tour function of English information in Moshan scenic spot was fully implemented ,which can meet the general needs of foreign tourists;the realization degree of the various English guided tour information functions was uneven;with the gradual increased of tourism participation level,tourists' objective perception degree of the required English information gradually decreased.According to the realization degree of the various English guided tour information functions,measures such as maintenance,promotion and creation should be taken.

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Study on tourists' satisfaction with night tourism in Wuhan city based on revised IPA analysis method

YAN Zhi-wu, LIANG Xiao-han, ZHENG Han-ni, LIU Ling

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 215-220. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.048

Abstract （410）

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In recent years,the development of night tourism in China was in full swing.However,through the investigation,it was found that there were still many problems in the development process of night tourism.Taking Wuhan city as the research object,this study introduced the revised IPA analysis method to evaluate the extended importance and tourists' satisfaction of Wuhan city night tourism.The results showed that tourists pay more attention to the price of leisure activities,the order of leisure activities,the price of shopping,communication facilities,public toilets and the convenience of obtaining tourism information,but their satisfaction was low.On this basis,this study put forward some suggestions from the aspects of enriching night tourism activities,maintaining the order of night tourism activities,improving the night tourism infrastructure,standardizing the price of night tourism products,in order to promote the sustainable and healthy development of night tourism in Wuhan city.

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Investigation and thinking on high-quality development of rice industry in Hubei province

CAO Peng, ZHANG Jian-she, CAI Xin, SUN Yang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 221-223. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.049

Abstract （440）

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Through long-term research and comprehensive consideration of natural resource conditions,economic development level,market potential demand and other factors,this study analyzed the problems existing in the development of rice industry in Hubei province,and put forward corresponding suggestions,in order to effectively improve the high-quality development level of rice industry in Hubei province.

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Enlightenment of Japanese agricultural model on the development of family farms in Handan city

LI Zhi-liang, HUANG Cong-lin, XING Hao-chun, LIU Xiao-bin, WU Zhong-yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 224-228. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.050

Abstract （495）

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Japan's agriculture model was characterized by small family farms.It adopted the policy of decentralized land possession and centralized and decentralized management,which adapted to the characteristics of small land area and limited cultivated land.Handan city in Hebei province also had the characteristics of more people and less land.Based on the field investigation in Yangqiao town,Daming county,Handan city,the problems existing in the development process of Handan city family farms were analyzed,and draw lessons from the experience of Japan in developing family farms,and put forward corresponding countermeasures ,so as to promote the healthy development of family farms in Handan city.

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Deconstructing the protection of rural traditional culture based on Marx's theory of spiritual production:Take Cangdong plan as an example

XIAO Han-zhen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (20): 229-233. DOI: 10.14088/j.cnki.issn0439-8114.2020.20.051

Abstract （448）

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In order to systematically deconstruct how to protect rural traditional culture,this study established the interpretation framework of spiritual production according to Marx's spiritual production theory.Taking Cangdong plan as an example,this study summarized the cultural heritage protection system of Cangdong village in different periods,explained the dilemma it faces and how to get rid of it,and refined the practical experience,so as to provide useful reference for the protection of rural traditional culture in rural revitalization strategy.

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Research progress and product development of Polygonatum sibiricum

YANG De, XUE Shu-jing, LU Qi, CHEN Xiao-chun, LI Lu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 5-9. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.001

Abstract （649）

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Polygonatum sibiricum is a medicinal and food homologous plant, rich in functional polysaccharides, flavonoids, steroidal saponins and other bioactive substances. It has the pharmacological effects of improving immunity, protecting kidney, regulating blood glucose and blood lipid. It has high medicinal value and important research significance, it is widely used in clinical and food therapy. The research progress on pharmacological action of Polygonatum sibiricum was reviewed, in order to provide reference for the further development of Polygonatum sibiricum.

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Application and research progress of chlorogenic acids in livestock and poultry production

CHEN Fang, DU En-cun, FAN Qi-wen, WEI Jin-tao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 10-13，16. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.002

Abstract （459）

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Chlorogenic acids （CGAs） as a class of ester compounds, are composed of caffeic acid and quinic acid by condensation. CGAs have biological activities such as antioxidant, antibacterial, anti-inflammatory, antiviral and regulation of glucose and lipid metabolism. The chemical structure, classification and the major plant source of CGAs were introduced. The research progresses of CGAs on its biological activity and application in livestock and poultry production was summarizedin order to provide reference for furtherdevelopment and utilization of CGAs.

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Application of gene editing technology in improving lean meat rate of pigs

REN Hong-yan, WANG Zi-jun, SHU Chang, HUA Zai-dong, BI Yan-zhen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 14-16. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.003

Abstract （454）

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The lean meat rate of Chinese local pigs is lower than that of foreign pigs. Transgenic technology can give new excellent traits to the species. With the rapid development of CRISPR / cas9 third-generation gene editing technology, gene-edited pigs have become the latest development trend in the pig industry. The research progress of gene editing technology in improving lean meat percentage of pork was reviewed.

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Breeding of three-line hybrid medium rice Xiangyou 5327 with early maturing and high yield

FANG Zhen-bing, CHEN Bo, ZHAO Sha-sha, PAN Gao-feng, FAN Bing, TIAN Yong-hong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 17-19. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.004

Abstract （431）

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Xiangyou 5327 is a new three-line hybrid medium rice combination with early maturing and high-yield, which is bred by Xiangyang Academy of Agricultural Sciences with three line sterile line Xiang313A and self bred restorer line R5327. It has the characteristics of early maturity, good yield, strong suitability and high seed production. In August 2019, it passed the approval by Crop Variety Certification Committee in Hubei province.

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Comparison of hybrid rice varieties for ratoon rice cultivation in southeast Hubei province

LI Yang, WANG Ben-fu, ZAHGN Zhi-sheng, CHENG Jian-ping

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 20-22. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.005

Abstract （542）

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For selection ratoon rice in southeast Hubei province, Y Liangyou No.2, Shenliangyou 5814, C Liangyou 4418, Guangliangyou 476 and Longliangyou Huazhan were chosen as materials. And the growing stage, yield and ratoon ability of these five varieties was investigated. The results showed that the performance of Shenliangyou 5814 was good than other varieties, and it was suitably planted as ratoon rice variety. The theoretical yield of Longliangyou Huazhan was high, but the growth stage was longer than other varieties and bad for yield of ratoon season.

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Status and trends of sweet potato research based on bibliometrics

WANG Chong, ZHANG Qi, WANG Lian-jun, YANG Xin-sun, ZHANG Wen-ying

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 23-29，150. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.006

Abstract （409）

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To provide reference for sweet potato [Ipomoea batatas （L.） Lam.] research work, the research status and development trend of sweet potato in the world was objectively analyzed using the literature database. Core collection database of Web of Science^TM was used to statistically analyze the research literature of sweet potato from 2009 to 2018 by bibliometrics methods. The results indicate that the output of research papers about sweet potato around the world was increased, and the quality of articles was improved year by year. The top 3 countries at numbers of papers of sweet potato were China, the United States and Japan. Developed countries such as American have the greatest influence in this field. Many highly cited papers were from these countries. In recent years, the research center is in Asia. Many productive and active writers came from China. However, there was still a gap from the world’s advanced level. The United States Department of Agriculture, Chinese Academy of Agricultural Sciences, China Agricultural University and other institutions have a greater influence on research papers on sweet potatoes. At present, the main research directions are molecular biology, genetics and food science. The cooperation between countries（regions） and subject has become increasingly closer.

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Effects of different fertilization treatments on wheat root rot and the diversity of fungi in rhizosphere soil

LIU Dong-hai, ZHANG Xue-jiang, WANG Peng, HU Cheng, QIAO Yan, ZHANG Zhi, LI Shuang-lai

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 30-34，50. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.007

Abstract （333）

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Four treatments were set up, including no fertilization （T1）, conventional fertilization （T2）, recommended fertilization （T3） and organic fertilizers replace 50% chemical fertilizers （T4）, in order to study the effect of different fertilization on the diversity of fungi and root rot of wheat rhizosphere soil. The results showed that the order of root activity of different fertilization treatments was T4, T3, T2 and T1 from big to small. The yield of recommended fertilization was 5 878.95 kg/hm²,only 66.45 kg/hm² lower than that of conventional fertilization,and 1 111.80 kg/hm² more than that of organic substitution. The use of chemical fertilizers reduced soil pH and the richness and diversity of fungal communities, which changed the structure of fungal communities. The relative abundance of Fusarium graminearum was T2, T4, T3 and T1 from large to small. The relative abundance of Bipolaris sorokiniana and the index of wheat root rot were all low in T4. Therefore, properly reducing the proportion of organic fertilizer replacing chemical fertilizer under the recommended amount of fertilizer can not only ensure the yield, but also reduce the incidence of root rot.

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Study on application effect of different controlled-release fertilizer on maize

ZHU Jin-ying, ZHANG Shu-liang, LI Sheng-dong, WANG Xu-qing, PEI Yan-ting

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 35-37，41. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.008

Abstract （874）

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A split block design experiment was carried out to study the application effect of three kinds of controlled-release fertilizer, including Jinnzhengda, Shikefeng and Zhushang, on summer maize cultivar Zhengdan 958, according to set three nitrogen amout levels of 288 kg/hm², 240 kg/hm² and 192 kg/hm². The results showed that the nitrogen use efficiency of Shikefeng was equal or higher than that of Jinzhengda and Zhushang, and the agronomical efficiency and partial productivity of Zhushang were higher than those of Jinzhengda and Shikefeng at the same nitrogen application rate. The application of special controlled-release fertilizer Zhushang was recommended on summer maize in the northern part of Shandong province, and the recommended amount of nitrogen was 240 kg/hm².

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Analysis of meteorological conditions of industrial tomato seedling based on solar greenhouse

WANG Yuan-yuan, YAO Wei, Bayincaicike, ZHOU Li-na, HUANG Jiu-jun, DONG Chao-yang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 38-41. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.009

Abstract （340）

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The meteorological conditions during the period of industrial tomato seedling cultivation in Xinjiang were studied to provide the meteorological basis for industrial tomato （Lycopersicon esculentum Mill.） seedling cultivation in greenhouse. Microclimate observatory stations in the shed of industrial tomato seedling in Hejing, Yanqi and Bohu of Bazhou in Xinjiang province were installed, and the leaf age and the physiological and ecological indexes of industrial tomato seedling was observed to summarize the light, temperature and water requirements from sowing to transplanting. The results showed that the tomato seedling shed standard were reached when the industrial tomato had grown 5 true leaves, achieved 45 to 52 days seedling age, and reached 12 cm to 15 cm of plant height. It took 8 to 10 days for tomato to grow from sowing to emergence. Time for one leaf to grow was 2 to 15 days. The greenhouse temperature was between 3.8 to 38.8 ℃ with the average temperature being 14.7 ℃ during the entire tomato growth period. The greenhouse temperature was between 5.8 to 36.6 ℃ with the average temperature of 11.8 to 22.9 ℃ during the first to fifth true leave growth period. The accumulated temperature of the whole seedling stage was 805.8 to 955.9 ℃.

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Adaptability research on the common meteorological drought indexes of SPEI and SPI in the northern Shaanxi province

LUO Man-ya, GENG Guang-po, ZHOU Hong-kui, ZHOU Xue

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 42-50. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.010

Abstract （532）

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Based on the meteorological data of 10 stations in northern Shaanxi province from 1981 to 2012, the temporal and spatial distribution of drought and the identification of typical drought events were studied by using two commonly used meteorological drought indexes of SPEI and SPI, and the drought historical records were used to verify the adaptability of the two drought indexes in northern Shaanxi province. The results showed that, on the annual scale, SPEI could more accurately describe the interannual variation of drought in northern Shaanxi province than SPI. It was found that the drought in northern Shaanxi province has intensified in the past 32 years. On the seasonal scale, the expressions of the two drought indexes were basically the same, and it was found that spring drought was the main drought in northern Shaanxi province. From the point of view of spatial distribution, both of them found that the southeast of northern Shaanxi province was a region with high incidence of extreme drought. In the identification of 28 typical drought events, 19 events were identified by SPEI and 6 events identified by SPI, and SPEI was more accurate than SPI. To sum up, SPEI is more suitable for drought monitoring in northern Shaanxi province.

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Analysis of flood-induced rainstorm and construction and validation of rainstorm forecast conceptual model in Xiangxi prefecture

SHAN Dan, WU Zhi-ke, ZHENG Fu-wei, MI Hong-bo

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 51-60，167. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.011

Abstract （354）

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Based on the daily precipitation data the 6 h reanalysis data of NCEP/FNL at 1°×1° from national weather stations and automatic meteorological stations in Xiangxi Tujia and Miao nationality autonomous prefecture, the rainstorms from 2013 to 2018 were analyzed. Rainstorm forecast conceptual model was built through the diagnosis of the characteristics of the situation field and the physical quantity and the samples of 2019 was tested. The results showed that during the heavy rain in Xiangxi perfecture, the high-altitude westerly jets were significant, and occurred under a single blocking condition. Weather systems like vortex and shear line concentrated distributed. There was positive correlation between precipitable water and rainstorm. The 700 hPa vertical velocity large value center can better indicate the regional heavy rainfal. The U component at 700 hPa was dominant, while the proportion of U and V components at 850 hPa was basically the same. Futhermore the change of the component has a good reference for the rainstorm forecast in Xiangxi perfecture. （Super） Low-level jets were conducive to the occurrence and maintenance of heavy rain, and special attention should be paid to the wind velocity fluctuation. Regional torrential rain was related well to the low-level convergence, the intensity and scope of the high-level divergence configuration, while the local rainstorm and forecast factors have good relationship.

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Change characteristics and correlation of air temperature and ground temperature in the middle area of Shandong province

XU Qing-wen, HAO Mao-sheng, WANG Feng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 61-64，133. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.012

Abstract （659）

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Using of 25 national meteorological stations in the middle area of Shandong province, the temporal and spatial variation characteristics and correlation of air temperature and ground temperature in this area were analyzed by the methods of linear trend estimation, Mann-Kendall mutation analysis,T-sliding and ArcGIS spatial interpolation. The results showed that the interannual variations of air temperature and ground temperature in the middle area of Shandong province were similar and showed an undulating upward trend, and the lowest and highest temperature appeared in the same time. The climate tendency rate of air temperature was slightly higher than that of ground temperature. The seasonal variation of air temperature was all upward. The ground temperature showed a downward trend in summer, and the other seasons showed an upward trend. The warming trend of ground temperature in winter was significantly higher than that of air temperature, but the warming trend in other seasons was lower than air temperature. The air temperature changed before and after 1997, and the ground temperature occurred around 2012. The spatial distribution of temperature showed that the overall trend of air temperature was low in the southeast, high in the northwest, and the warming trend was fast in the east and slow in the west. The trend of ground temperature showed high in the west, low in the Changyi-Weifang-Changle-Wuyuan area, and the warming trend was fast in the north and slow in the south. There was an negative correlation between the air temperature and the longitude, and climate tendency rate of air temperature was positively correlated with the longitude. The level of ground temperature was negative related to longitude, and its climate tendency rate was also negative related to latitude. The months ground temperature and air temperature were extremely positively correlated. The correlation degree in the winter half year was higher than that in the summer half year. The month difference between ground temperature and air temperature was increasing month by month in the first half of the year, and decreasing month by month in the second half of the year.

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Study on air quality in China’s main cities based on entropy weight TOPSIS method

WANG Wen-yin, WANG Ya-ru

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 65-68. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.013

Abstract （461）

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In order to objectively and comprehensively evaluate the air quality of each city in China and formulate effective prevention and control measures, the six indexes of SO₂,NO₂,PM10,O₃,CO and PM2.5 were selected as evaluation factors, and entropy weight method was adopted to empower each index. Combined with TOPSIS method, the air quality evaluation model was established, and the air quality ranking of 20 major cities was obtained. The results showed that the three major pollutants were SO₂,CO and PM2.5, and the air quality of Shenzhen, Ningbo and Shanghai was better than others. In view of pollution, some suggestions for reducing carbon emissions, popularizing clean energy, strengthening protection measures, paying attention to environmental protection publicity and strengthening supervision mechanism were put forward.

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Study on the suitability of land reclamation in subsidence area based on R/S analysis and limit learning machine

CHEN Qiang, HUANG Xin, Lahuancairang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 69-74. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.014

Abstract （328）

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In order to solve the problem of land destruction caused by mining subsidence and to maintain the ecological environment stability of the mining area, the study on the suitability of land reclamation in subsidence area was carried out based on the theory of R/S analysis and limit learning machine. Firstly, the stability status of subsidence area was judged by R/S analysis, and the evolution law of subsidence stability with time was analyzed. Then, the prediction model of subsidence deformation was built by using limit learning machine to evaluate the development trend of subsidence deformation, so as to provide basis for land reclamation. Finally, the study on land reclamation measures in the subsidence area was carried out combined with the characteristics of coal mining subsidence. The case study showed that the R/S analysis could effectively evaluate the subsidence stability of the subsidence area, and that there were some differences in the subsidence stability of different stages, but the stability tended to increase with time. At the same time, through the prediction of subsidence deformation, the relative error mean value of the prediction results of the prediction model was less than 3%, which has high prediction accuracy, and the prediction model was verified. The subsidence deformation tended to be stable in the later period, and the stability evaluation was integrated to obtain the appropriate land reclamation measures to be taken in the future. Finally, the land reclamation in the subsidence area is a systematic work, and it is imperative to grasp the reasonable reclamation opportunity through the settlement stability evaluation, and reasonably standardize the reclamation measures in combination with the reclamation conditions of the mining area, to improve land use efficiency.

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Study on equilibrium and kinetics of methylene blue adsorption of magnetic rice straw

TENG Tao, CAO Li-min, LIU Jian-hui, DAI Guang-rong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 75-80. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.015

Abstract （372）

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In order to provide the theoretical basis of magnetic rice straw （MRS） applied in printing and dyeing wastewater treatment, the effect of process parameters such as temperature, contact time, initial concentration of MB and the dosage of MRS on MB adsorption were discussed by static adsorption method, and the isothermal adsorption and kinetic model were also examined. Furthermore, the MRS was characterized by scanning electron microscopy （SEM）. The results showed that the adsorption rate could be up to 97.93% under following conditions： the temperature was 45 ℃, the contact time was 40 min, the initial concentration of MB was 0.05 g/L, and the dosage of MRS was 0.10 g. And the adsorption data followed Langmuir isotherm model （R²>0.94） and pseudo-second-order kinetic model （R²>0.99）. It revealed that the chemisorption nature of MRS on MB, and the adsorption process was easy to occur. SEM results showed that MRS surface had a significant change, and Fe₃O₄ was bound to the straw in the form of tiny particles. Therefore, MRS as a new type of absorbent which not only has high adsorption efficiency, but also has a good solid-liquid separation performance could be used in the treatment of printing and dyeing wastewater.

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Effects of activated carbon-polytetrafluoroethylene electrodes on NH₄⁺-N removal in bioelectrochemical system

WANG Rui, ZHANG Dong-dong, GUO Peng, WANG Yi, ZHANG Chun-fang, CHEN Lei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 81-87. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.016

Abstract （397）

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Carbon black and polytetrafluoroethylene （PTFE）were flat-pressed to fabricate the activated carbon （AC）-polytetrafluoroethylene electrodes. These AC-based electrodes were applied in a single-chamber BES to achieve autotrophic NH₄⁺-N removal under organic carbon-free conditions. The results showed that NH₄⁺-N removal effect could be enhanced by applying voltage to the BES, in which case the NH₄⁺-N removal efficiency gradually increased as the applied voltage increased from 0.10 V to 0.25 V. The average NH₄⁺-N removal rate in a BES was 47.8 mg/（L·d）. Cyclic voltammetry results revealed the presence of several redox-active components on the anode and cathode surfaces, indicating that the AC-based bioanode and biocathode had good electroactivities. Microbial community analysis of 16S rRNA genes based on high-through put sequencing indicated that the diversity of the microbial community increased after electric power application, and Pseudomonas and Paracoccus could be important for nitrogen-compound removal in the BES.

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Analysis of aethesatic connectivity among architechture,music and mathematics during landscape design in ancient Greek and Baroque periods

DU Qing-yao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 88-94，98. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.017

Abstract （317）

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Architecture and music are inextricably linked no matter from internal structure or external form of expression, as the most dominant and leading basic discipline, mathematics is always using its incomparable logic and the axiomatic collection model to influence and promote the development and transformation of architecture and music creation. Based on the theoretical knowledge of natural science and aesthetics, taking the most representative ancient Greek and Baroque periods in the history of natural science and culture in the West as examples, combining visual pictures and digital models to explore a variety of translation models between mathematics, architecture and music, summarize the theories and characteristics of the aesthetic connection of architecture, music, and mathematics in different periods, to provide new ideas and application models for better realization of cross-border design.

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Study on flower bud differentiation of Rosa damascene

LI Li-yun, LIU Xing-le, YANG Qing, YU Hua-qiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 95-98. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.018

Abstract （468）

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This study was conducted from December 11, 2018 to April 15, 2019. A total of 250 apex buds were sampled. The morphological characteristics of buds of Damascus rose at different periods were observed by paraffin section. The statistical results showed that the differentiation of rose bud in Damascus began at the end of January, and completed from the end of march to the beginning of April, lasting about 2 months. The flower bud differentiation can be divided into six stages, namely, the cone differentiation stage of reproductive growth, sepal primordial differentiation stage, petal primordial differentiation stage, stamen primordial differentiation stage, pistil primordial differentiation stage and differentiation completion stage. Flower buds began to differentiate at 5 ℃, the differentiation rate was accelerated at 10~15 ℃, and the differentiation was completed at 15~20 ℃.

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Effects of substrate and plant growth regulator on cutting of Saintpantia ionantha

YAN Hai-Xia, TAO Da-yan, GUAN Shi-kai, ZHOU Jin-ye, SONG Qian, LU Jia-shi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 99-101. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.019

Abstract （356）

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To study the factors that affect the survival of Saintpantia ionantha’s cutting, and to provide the basis for its cutting production in the future. Taking the leaves of Saintpantia ionantha as materials, the single factor cutting experiments of 4 kinds of different substrates, 3 kinds of plant growth regulators and 2 kinds of plant growth regulators with different concentrations were carried out to analyze the effects of substrates, types and concentrations of plant growth regulators on rooting rate, sprouting rate and number of new buds, and to select the best cutting substrates and concentrations of plant growth regulators. The results showed that the rooting rate, sprouting rate and number of new shoots were the highest when the volume ratio of peat, perlite and river sand was 2∶2∶1, which were 98.00%, 25.82% and 19.00, respectively. It was the best cutting medium. The effects of different plant growth regulators vary with species, among which IBA and NAA can significantly improve the survival effect. The concentration of IBA and NAA in the range of 100~400 mg/L had no significant effect on the rooting rate, but had significant effect on the sprouting rate and the number of new buds. The best concentration of IBA and NAA in cutting propagation was 200 mg/L.

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Study on the effects of different nutrient concentrations on growth of grape and substrate under root restriction

JIN Li, WANG Yan, PEI Xian, YANG Shou-kun, YAO Zhong-hua, LI Chang-lin, CHEN Xu-bai, SU Fu-yuan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 102-105，116. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.020

Abstract （395）

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A experiment with annual grape of Shine Muscat and Yongyouyihao was conducted to study the influences of different nutrient solution and identity the appropriate nitrogen content for grape. The results showed that 30 mg/L nitrogen treatment can promote growth for Shine Muscat and 180 and 120 mg/L nitrogen treatments can promote growth for Yongyouyihao. Shine Muscat is more efficient to use nutrition. With less nutrition, the nitrogen is left less in the substrate under root restriction for Shine Muscat. Yongyouyihao is less efficient to use nutrition. With more nutrition, the nitrogen is left less in the substrate under root restriction for Yongyouyihao.

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The analysis and fitting of growth curve of Jianghan chicken

WU Yan, PAN Ai-luan, PI Jin-song, SHEN Jie, ZHANG Hao, PU Yue-jin, LIANG Zhen-hua, DU Jin-ping, SUN Jing, HUANG Zhu-lin, WANG Xiao-zhong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 106-109，112. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.021

Abstract （426）

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Jianghan chicken is a good local chicken of Hubei. The study of early growth rules of Jianghan chicken provide an evidence for new lines breeding. Three kinds of nonlinear model （Logistic, Gompertz and Von Bertalanffy） were used to analyze the growth curve of Jianghan chicken from 0 to 20 weeks. The results showed that the growth curves were appropriately fitted with the three models. Compared the three fitted results, the Gompertz model had the best effect on fitting with the growth curves of Jianghan chicken （R²≥0.996）, the growth inflexion was 9.170 weeks and 763.070 g for male chicken and 9.355 weeks and 623.185 g for female chicken. It was feasible to analyze and fit the growth curve of the Jianghan chicken using the three models. Through the fitting of the growth curve, we can master the growth and development patterns of Jianghan chicken in time and find the problems in feeding and management.

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Identification of the mutations in porcine MMP20 gene and its association analysis with reproduction traits

WU Jun-jing, DONG Bin-ke, QIAO Mu, MEI Shu-qi, PENG Xian-wen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 110-112. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.022

Abstract （336）

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In the present study two mutations （g.14334bpC>G and g.14369bpC>T） at intron 3 in porcine MMP20 gene were identified by Sanger sequencing. These two mutations were completely linked, and the association results showed that they significantly affected the reproduction traits （TNB, TNA, NAd21, BW and LW） in Large White pigs （P<0.05）. GG/CC genotype sows had the lowest litter size, followed by GC/CT genotype sows, while CC/TT genotype sows had the highest litter size.

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Effect of ventilation rate on fragrance transfer rate in cigarette filter

XIAO Wei-yi, LI Xiao, XIONG Shan-shan, HE Xue-feng, HUANG Hai-qun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 113-116. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.023

Abstract （365）

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In order to investigate the effect of cigarette ventilation rate on the transfer rate of phenylethyl alcohol and ethyl vanillin in cigarette filters containing alcohol and water systems, the content of phenylethyl alcohol and ethyl vanillin were determined by cigarettes with different ventilation rates and different flavors. The results showed that the release amount and transfer rate of the essence in the alcohol system were slightly higher than those in the water system. With the increase of the amount of phenylethyl alcohol in the filter, the transfer rate of phenylethyl alcohol showed an upward trend, and the amount of flavor added and the transfer rate showed a significant positive correlation. With the increase of ventilation rate, the transfer rate of phenylethyl alcohol in cigarette samples showed a gradual upward trend, and the transfer rate of phenylethyl alcohol showed a significant positive correlation with the total ventilation rate.

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Comparison of properties of three starch based edible films

QIN Han-zhen, MAO Jian-ke, YU Wen-yi, TAO Yang-yun, XU Ping, HU Pei-pei, YU Zuo-long

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 117-120. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.024

Abstract （455）

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The properties of edible films prepared from raw starch, soluble starch and water-soluble starch were studied. The gelatinization characteristics, contact angle and mechanical properties, permeability, crystallinity and microstructure of three kinds of starch solutions and films were investigated. The results showed that soluble starch film solution and water-soluble starch film solution did not show gelatinization. With the increase of solubility, the contact angle between the three kinds of starch film solution and the plastic plate decreased gradually. With the decrease of the molecular weight of starch, the tensile strength of the three kinds of starch film increased first and then decreased sharply, and the fracture elongation increased gradually. The permeability of salad oil, water vapor, CO₂ and O₂ decreased was studied, it was found that the permeability of water-soluble starch film was the lowest. The oil permeability, water vapor permeability, CO₂ permeability and O² transmittance were 0.084 g·m/（m²·d）, 2.360 g·mm/（m²·d·kPa）, 0.320 g/d and 0.023 g/d, respectively. There were great differences in crystallinity and microstructure of the three kinds of starch films. Therefore, the modification of starch molecules significantly affects the properties of starch products and provides technical support for the development and applications of starch.

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Effects of 1-MCP treatment on shelf life quality of Cuiguan pear

ZHANG Jing-guo, CHEN Qi-liang, YANG Xiao-ping, FAN Jing, HU Hong-ju

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 121-123. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.025

Abstract （356）

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Pyrus prifolia Nakai cv Cuiguan was used to investigate the effects of 1-Methylcyclopropene treatment with three different concentrations on postharvest shelf life at room temperature. The results showed that 1-MCP treatment could significantly reduce the respiratory intensity and ethylene release of Cuiguan pear, inhibit the peel color turning yellow and maintain the firmness of pulp and improve the rate of good fruit, and significantly extend the shelf life of Cuiguan pear to more than 20 days at room temperature, while the shelf life of Cuiguan pear was only about 10 days. 0.5 μL·L^-1 1-MCP treatment could meet the storage requirements of 20 days shelf life at room temperature.

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Determination of 46 banned pesticide residues in Cissus hexangularis and Ixeridium chinense by GC-MS/MS

TANG Ming-hua, JIANG Yong-ning, XU Meng, LU Gui-jian, LIANG Shuang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 124-129，154. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.026

Abstract （336）

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The forbidden,restricted,high-toxic,high-risk and customary pesticides were picked out asdetecting indexes according to the principals of risk management. 20 batches of Cissus hexangularis and Ixeridium chinense samples collected in different areas were detected. The factors affecting the extraction,purification and detection were optimized and the final condition was established as improve the accuracy of results. The results showed that the linear relationship of each component in the corresponding range was good in 50~500 ng/mL, the correlation coefficient ≥0.99, and the average recovery rate of pesticides was 48.6%~119.0%, which was in line with the trace detection requirements. The detecting indexes in the method cover most of the forbidden and restricted pesticides which are suitable for gas chromatography-tandem mass spectrometry, which is meaningful for the safety supervision of the Cissus hexangularis and Ixeridium chinense. With the advantage of rapidness and accuracy, this method can be used for routine determination of multi-pesticides in Cissus hexangularis and Ixeridium chinense.

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Determination of six inorganic elements in mainstream cigarette smoke by ICP-MS

HAN Wei-qi, WANG Yang, SUN Ming, JIANG Ying, LI Feng-qin, LIU Wen-chun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 130-133. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.027

Abstract （417）

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Establish a method to determination six inorganic elements in mainstream cigarette smoke by ICP-MS. The particulate phase was collected by quartz filter pad, and the gas phase was collected by 5% nitric acid （volume fraction） which as absorbent, and then treatment the quartz filter pad by microwave digestion, different from other methods, the whole filter was digested directly in this method, the digestion acid system was as follows： HNO₃∶HCl∶HF（6∶1∶2）, digested completely, and high extraction, determined byICP-MS with more accurate results. The results showed that correlation coefficients of the calibration curves of the six elements were all above 0.999, and the detection limits were between 1.57~2.51 ng per cigarette, the recoveries were from 91.2% to 107.0%. This method is accurate, quick and simple, and suited to the determination of As, Pb, Cd, Cr, Ni, Se in mainstream cigarette smoke.

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Determination of 5 benzenes （BTEX） and 17 phthalates esters （PAEs） in electronic cigarettes liquid by GC-MS

DING Cheng, LI Jian-zheng, YANG Wen-bin, XU Xiao, YUAN Tao, YANG Hai-yu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 134-139. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.028

Abstract （417）

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A GC-MS（gas chromatography-mass spectrometer） method was developed for the simultaneous detection of five benzenes（BTEX） and 17 phthalates esters（PAEs） in electronic cigarette liquid. The linear range of the analytes was from 0.05~1.00 μg/mL with R² 0.995 0. The LOD and LOQ is 0.001~0.010 μg/mL and 0.003~0.050 μg/mL respectively. The GC-MS method displayed a recovery of 81.64%~110.48%, and the RSD is 0.55%~9.93%. Compared to the reported method, GC-MS method holds the merits of easy pretreatment, low LOD and LOQ, rapid analysis, and low solvent consumption.

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Determination of 8 residues of herbicide and mycotoxin in wheat by UPLC-MS/MS

ZHAO Jian, CHEN Guo, ZHANG Hao, WU Yin-liang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 140-143. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.029

Abstract （358）

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A method was developed in wheat by UPLC-MS/MS for determining 8 residues of herbicide and mycotoxin of isoproturon,MCPA,tribenuron methyl,bensulfuron methyl,fumonisin B₁, fumonisin B₂, sterigmatocystin,T-2. The wheat sample was extracted with acetonitrile-water-formic acid （85/14/1, V/V/V）,purified by appropriate amount of PestiCarb. The purified solution was concentrated, redissolved and centrifuged. 8 drugs were separated on ACQUITY UPLC BEH Shield RP18 column by gradient elution, then analyzed by ESI in positive and negative mode under MRM mode. The results showed that the linear range was from 2 to 100 μg/L with the correlation coefficients above 0.99 for all the 8 drugs. The average recoveries of the 8 drugs ranged from 76.4% to 114.0% and the RSD were 1.8%~9.2% in spiked wheat at three levels. The limits of quantification were 0.4~2.0 μg/kg. The method is suitable for the simultaneous determination 8 residues of herbicide and mycotoxins in wheat.

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Determination of cyadox in milk by UPLC-MS/MS

MA Xiao-nian, ZHANG Xiu-qing, CHEN Jun-xiu, OU Li-hua

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 144-146. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.030

Abstract （341）

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To establish a reliable pretreatment method for cyadox in milk with UPLC-MS/MS. The sample was extracted by ethyl acetate, then the results was analysed after PEP cartridge purification. The objective compounds were separated using Inertsil ODS-3column（3 μm,2.1 mm×100 mm）with acetonitrile/ methanol（3∶11） -water （0.1% formate） as mobile phase and analyzed by mass spectrometry in the positive electrospray ionization under multiple reaction monitoring mode（MRM）. The results showed that PEP cartridge had good recovery rate. The detection limit of cyadox was 0.01 μg/g, limit of quantitation was 0.04 μg/g, the average recoveries for spiked levels of 0.384, 0.960,1.920 μg were 136.0%, 116.0%, 62.6%. The method is suitable for the detection of cyadox residual in milk.

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Determination of 19 organophosphorus pesticide residues in Citrus reticulata peel based on QuEChERS-GC method

ZHANG Xian, HU Xi-zhou, PENG Xi-tian, WANG Xiao-fei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 147-150. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.031

Abstract （367）

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To establish a method of QuEChERS combined with GC for detection of the 19 kinds of pesticide residues in Citrus reticulata peel. The sample were extracted by acidified acetonitrile, purified by QuEChERS method, detected by flame photometric detector and quantified by external standard method. The results showed that the good linear ranges of 19 organophosphorus pesticides were 0.050 0~0.500 0 μg/mL and the correlation coefficients were 0.994 1~0.998 9. The average recoveries were in the range of 80.4%~100.2%. The relative standard deviations were between 1.2% and 4.8%. The limits of quantification were in range of 0.010 0~0.020 4 μg/mL. The method is simple, sensitive and can be used for determination of 19 organophosphorus pesticides in Citrus reticulata peel.

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Analysis the nutrient compositions of Siraitia grosvenorii in the hilly region at north Guangxi

ZHANG Yan-zhao, XU Guang-ping, ZHOU Long-wu, TENG Qiu-mei, HE Wen, ZHANG Zhong-feng, ZHANG De-nan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 151-154. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.032

Abstract （445）

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The nutrient compositions extracted from Siraitia grosvenorii in the middle and high altitude hilly region at northern Guangxi （Longsheng autonomous county, Guilin city） were analyzed and compared with Yongfu and Xiangxi county, contents of ash, total sugar, protein, crude fiber, fat, vitamin C（VC）, vitamin E（VE） and amino acid were determined. Results showed that the contents of ash, total sugar, protein, crude fiber, fat, VC and VE were （7.59±1.05）%, （59.99±6.33）%, （21.26±1.68）%, （63.25±5.01）%, （0.09±0.02）%, （38.00±3.06） mg/100 g and （0.61±0.02） mg/100 g. Contents of protein, VE and fat from Siraitia grosvenorii in Longsheng were higher than that of Yongfu with 7.37%, 3.39% and 12.50%, the contents of total sugar, crude fiber and ash from Siraitia grosvenorii in Longsheng were 59.99%, 63.25% and 7.59% which were lower than Yongfu with 66.70%, 69.16% and 8.72%. The contents of ash, total sugar, protein, crude fiber and VC in Siraitia grosvenorii of Longsheng were 1.61, 1.99, 2.46, 1.38 and 4.85 times of the maximum contents of related components in Xiangxi county. There were 17 kinds of amino acids in Longsheng Siraitia grosvenorii, contents of total amino acids were 8.20%, 7 kinds of human body essential amino acids were 31%. There was no significant difference in contents of amino acids in Siraitia grosvenorii between Longsheng and Yongfu. Siraitia grosvenorii is rich in nutrient,which has high protein content, amino acid and VC. It shows higher nutritional value and is suitable for expanding and large-scale planting in the middle and high altitude hilly region at northern Guangxi.

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Determination and evaluation on content of nutrient component and heavy metal of water caltrop

LI Shuang-mei, KE Wei-dong, PENG Jing, HUANG Xin-fang, ZHONG Lan, WANG Chun-li, LI Ming-hua

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 155-157. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.033

Abstract （333）

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Determination and evaluation on content of 13 kinds of nutrient component and 4 kinds of heavy metal in Hanchunred water caltrop and Jiayuwater caltrop were carried out. It indicated that,nutrient component content of Hanchunred water caltrop was higher than that of Jiayuwater caltrop in its totality;Water caltrop stem containted more content of natrium （Na）, ferrum （Fe）, manganese （Mn）, calcium （Ca）, magnesium （Mg）, dry matter, crude fiber and fewer content of protein, potassium （K）, zinc （Zn） and phosphorus （P）, comparing with 104~140 kinds of fresh vegetable. Content of selenium （Se） wasn’t check out. Content of plumbum （Pb）, arsenic （As）, cadmium （Cd） and mercury （Hg） of water caltrop stem was justified National Food Safety Standard.

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Study on the control method for Zhuang medicine Flueggea virosa

LU Zheng-lin, HUANG Rui-song, LIANG Zi-ning

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 158-162. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.034

Abstract （330）

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To establish the quality standard for Flueggea virosa, fifteen batches of Flueggea virosa were collected, the character and microscopic characteristic were studied; TLC method was used to identify these herbal medicines, the spots were clear and well-separated; The moisture of fifteen batches samples were 10.3%~11.3%, the total ashe content were 2.6%~5.0%, acid-insoluble ash were 0.04%~0.40%, the content of ethyl alcohol-soluble extract were 9.4%~13.4%; The contents of bergenin which determined by HPLC were 0.096%~1.430%. This proper and accurate method can be used for the quality control of Flueggea virosa.

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Spectroscopic studies of interaction between melamine and bovine serum albumin

YANG Mei-ling, CUI Dong-ya, NIU Run-hua

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 163-167. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.035

Abstract （350）

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The interaction between melamine and bovine serum albumin （BSA） was investigated by using fluorescence spectroscopy, UV absorption spectroscopy and circular dichroism spectroscopy with the simulated physiological condition. The experiment results of UV absorption spectroscopy and fluorescence spectroscopy exhibited that melamine and BSA could form a kind of new compound that could be proved through the fluorescence quenching of the compound of BSA and melamine. It was the static fluorescence quenching. According to the calculation, at 28, 32 and 37 ℃, the quenching constants were 1.618×10¹², 1.614×10¹², 1.620×10¹²L/（mol·s）, respectively; The binding constants were 2.64×10³,7.59×10³ and 9.80×10³ L/mol, respectively; And the number of binding sites was 1, approximately; And the binding distance was 3.7 nm. All these indicated that a new chemical compound was produced in the mixture of BAS and melamine, and a special binding sit of BAS could only bind a one melamine molecule, and the binding distance between different sites was 3.7 nm. The results of circular dichroism displayed that melamine had a great effect on the secondary structure of BSA.

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Effect of soil cover device for sowing of integrated machinery of cultivation and fertilization on the emergence and yield of Sesamum indicum

WEI Lin-gen, LYU Feng-juan, WANG Rui-qing, XIAO Yun-ping, HUA Ju-lin, LI Jian-guo, ZHANG Zhi-hua, HUANG Hui-qin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 168-170，183. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.036

Abstract （381）

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In order to explore the high-efficiency and high-yield mechanized sowing mode of Sesamum indicum in hilly and sloping land, the effect of two kinds of sowing and mulching devices on the emergence and yield of sesame was studied. The results showed that it was better to plant sesame seeds with no ditcher to cover the soil before rain with more rain season to obtain a higher seedling rate; However, to plant sesame seeds after rain in the dry season with furrow opener.

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Design of small intelligent irrigation system based on singlechip microcomputer

SUN Xiao-chun, SUN Xiao-ying

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 171-173. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.037

Abstract （488）

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51 singlechip microcomputer was used as the controller,according to the requirements of crop growth characteristics, a reasonable humidity parameter range was set. The singlechip microcomputer collects, analyzes and processes the soil humidity information, and then controls whether the pump motor pumps water for irrigation. At the same time, it displays the soil humidity data in real time, and finally realizes the design of intelligent water-saving irrigation system. After testing, the system can monitor the data in real time and realize the requirements of intelligent irrigation.

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Development of early warning system for feeder based on big data technology

LU Ji-ping, ZHANG Zhong-shu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 174-176. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.038

Abstract （403）

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It can be found through the analysis of the failure of the feeding system in the process of leaf moistening and feeding,the abnormal operation of the feeding pump often occurs before the failure of the feeding system, because this parameter was not the key assessment index, and the failure symptom was not obvious, with transient jump, this phenomenon has not been caught by the relevant personnel, resulting in the gradual deterioration of the equipment operation status, resulting in the failure of the feeding system, or even the shutdown. Using big data analysis to summarize and summarize the equipment management of the original feeding module of the feeder, so as to establish the early warning model, develop an intelligent equipment early warning system, find the fault point in time and quickly, and achieve accurate prediction and maintenance. The results show that the time of fault diagnosis can be reduced to 20 minutes, which greatly shortens the time of fault diagnosis and improves the production efficiency of the workshop.

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Research on the effect of meteorological factors on Camellia oleifera yield based on decision tree algorithm

HUANG Chao, LIAO Yu-fang, JIANG Yuan-hua, PENG Jia-dong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 177-183. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.039

Abstract （421）

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In this study, two kinds of decision tree algorithms, CART（Classification and regression tree） and CHAID（Chi-Square automatic interaction detection） were adopted to simulate the yield of Camellia oleifera of Hunan province based on meteorological factors of different phenological phase over from 2010 to 2016. The results showed that the average relative errors of CART and CHAID algorithms were 8.80% and 14.30% respectively, and the trend accuracy were 97.40% and 92.20% respectively. The accumulated temperature above 0 ℃ and the average maximum temperature at flowering stage had the greatest influence on Camellia oleifera yield. In the first fruit expansion period and the peak of oil transformation and accumulation, the diurnal temperature range, the mean minimum temperature and high temperature days were the most important. It is of great significance for improving the efficiency of Camellia oleifera industry chain.

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Study on spatial pattern and optimization of ecological recreation in Guilin city

LIU Meng-yuan, WU Guo-quan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 184-188. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.040

Abstract （340）

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With the improvement of urbanization level, Guilin residents’ demand for urban recreation is constantly increasing, the city and its surrounding areas need a large amount of recreation space for people’s leisure use. This study analyzed the overall layout and formation mechanism, land type and space distribution of Guilin’s urban recreation areas, used scientific methods to analyze regional tourism supply and demand, summarized and sorted out the general rules of regional optimization, it provides a reference for the design and development of Guilin’s urban tourism products.

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Research on the protection and utilization of the cultural heritage of the Silk Road in Shaanxi province from the perspective of cultural soft power

DONG Xiao-ying

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 189-191，195. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.041

Abstract （426）

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Shaanxi province is big province of cultural heritage of the Silk Road, and Xi’an city is the starting point of the ancient Silk Road. This study compared and analyzed the cultural soft power of the Silk Road cultural heritage in Shaanxi province, found out the advantages and disadvantages, and put forward the protection and development path of the Silk Road in the context of cultural soft power with a view to build Shaanxi province into an economic and cultural highland of the Silk Road.

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Research on legal issues of agricultural products e-commerce live broadcast

ZHU Xuan, YAN Xiao-li

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 192-195. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.042

Abstract （543）

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As a product of the internet development in the new era, e-commerce live broadcast of agricultural product has greatly expanded the marketing of agricultural products, and helped to promote the revitalization of rural areas and achieve farmers’ poverty alleviation. However, under different circumstances, the identity of the main body of the live broadcaster and the e-commerce live broadcast platform is complex and complex. Only when the legal status of the main body of both parties is clear, can the corresponding legal responsibilities be determined. This article specifically analyzed the legal status and responsibilities of anchors and e-commerce platforms through the classification of different types of agricultural products live broadcast goods, and put forward suggestions for improving the supervision of e-commerce live broadcasts, in order to promote the healthy development of the agricultural products e-commerce live broadcast industry.

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Empirical research on the promotion of agricultural product brand competitiveness by experiential marketing

GAO Hai-qin, LI Mei-gui, CUI Guan-yun, YANG Shuai

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 196-200，222. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.043

Abstract （360）

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Experiential marketing is a new brand marketing model that is defined and designed based on consumers' senses, emotions, thinking, actions and associations. Taking the agricultural experience, exhibition experience and festival experience marketing models adopted by Shouguang vegetables as an example, the evaluation index system of agricultural product brand competitiveness was constructed, using factor analysis method to compare and analyze the effects of different experience marketing models on agricultural product brand competitiveness, and using analytic hierarchy process to extract the most effective influencing factors of experiential marketing on the competitiveness of agricultural product brands, and provide reference development suggestions for enhancing the competitiveness of agricultural product brands in China.

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Present situation and prospect of soybean production in China

WANG Yu, LI Gan-qiong, YU Wen, FENG Yao, ZHONG Xin, LIU Ran, XU Shi-wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 201-207. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.044

Abstract （562）

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Based on the historical data of soybean from 1949 in China, this study analyzed the variation law, distribution characteristics and fluctuation period of soybean yield in detail, sowing area and per unit yield all regions, discussed the important factors, which affected soybean production in China, and established the prediction model of soybean per unit yield in China （index fitting curve, linear fitting curve and polynomial fitting curve） to analyze the future soybean production in China from 2020 to 2035. It is found that the increase of soybean production in China mainly comes from the increase of per unit yield since the founding of the people’s Republic of China; Compared with other food varieties, the soybean production in China has the characteristics of relatively concentrated area, high fluctuation frequency, short fluctuation period and large fluctuation range; It is estimated that China’s soybean production will reach 25 million tons in 2030 and 27 million tons in 2035, which is lower than that of the United States, Brazil and other countries, but far higher than that of Canada, Australia Asia and India.

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Research on the countermeasures of speeding up the cultivation of leading enterprises of rapeseed oil in Hubei province

JIANG Bo, CHENG Tai, WANG Ying, DENG Yin-xia, YING Liang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 208-210. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.045

Abstract （356）

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Rape industry in Hubei province has obvious production advantages, but there are some problems in the development of leading enterprises of rapeseed oil, such as excessive processing capacity, large import impact, difficulty in financing, lack of big leader and big brand driving. Therefore, we should learn from the successful experience of other provinces, combine with the reality of Hubei province, seize the opportunity and implement comprehensive policies, to speed up the cultivation of rapeseed oil leading enterprises, and make “Hubei contribution”for the safety of edible oil supply in China.

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Research on the fit between the supply of rural public goods and the development of rural economy

XU Qin-zeng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 211-214. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.046

Abstract （416）

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Rural public goods are the most important guarantee of rural economic development. The effective supply of rural public goods can truly reflect the actual needs and development goals of villagers in various areas in terms of scale, structure and form. Starting from the compatibility of rural public goods supply and rural economic development, the fusion mechanism between rural economic development and rural public goods supply was analyzed,the fusion strategy was put forward.

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Legal thinking on China's grain security under the background of Sino-US trade friction

GUO Xiao-rui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 215-217. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.047

Abstract （429）

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Since the occurrence of trade friction between China and the United States, domestic grain imports have declined and prices have fluctuated. Grain security has once again aroused concern from all walks of life. China’s grain security was facing the challenges of increasing grain demand gap and urgent transformation and upgrading of major grain producing areas. In addition to the rigid constraints such as the shortage of arable land and water resources, the imperfect legal system of grain security was one of the main reasons. Specifically, we should speed up the introduction of relevant laws on grain security, optimize the “Separation of Three Powers” system, and promote the development of ecological agriculture law.

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Analysis of the mechanism and path of poverty alleviation of e-commerce based on SWOT： Take Baisha county Hainan province as an example

YANG Er-li

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 218-222. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.048

Abstract （416）

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With the development of the internet,e-commerce poverty alleviation has become a brand-new poverty alleviation method,and has achieved remarkable results in some areas. However, at present, e-commerce poverty alleviation is still in the exploratory stage,and there are many problems to be solved in the application process. This study took Baisha county of Hannan province as the research object,and used SWOT analysis theory to analyze the internal advantages,disadvantages,external opportunities and threats of e-commerce poverty alleviation in Baisha county of Hanan province, and put forward the corresponding development strategy and path.

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Practice and reflections of village movement management mode in China under the COVID-19

TANG Yi-ming

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 223-227，232. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.049

Abstract （531）

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COVID-19 epidemic prevention and control in China has achieved success in the exercise of “hard core” campaign. It is caused by many factors such as the severity of the epidemic, the limited resources of the countryside, the cultural tradition and the objectivity of the regional environment. However, the sports governance model showed some disadvantages, and the measures were blind and radical, which has produced certain side effects. Through the analysis and reflection on the practice of rural epidemic prevention and control and the endogenous mechanism of mobile governance mode, based on the synergistic effect of autonomy, rule of law and rule of virtue, an efficient and normalized rural governance model was constructed, in order to further improve the mechanism of rural response to crisis and enhance the efficiency of rural governance.

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New ethical construction of rural society in the new era

MA Zhi-fan, DAI Rui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (21): 228-232. DOI: 10.14088/j.cnki.issn0439-8114.2020.21.050

Abstract （396）

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Rural ethics in the new era is the stabilizer of the village, the accelerator of rural revitalization, and the spiritual home of farmers,the development of rural ethics provides a steady stream of spiritual power for rural revitalization and is the soul of rural revitalization. With the development of modern civilization, rural society has undergone a series of changes in the main contradictions, family structure, and production methods. It is necessary to re-examine the ethical problems faced by rural development in the new era from a historical and dialectical perspective. It analyzed that the rural society in the new era was facing the invasion of modern ideological trend, the fracture of traditional blood geographical relationship, the collapse of moral authority and the “double standard” concept of benefit and righteousness. The task of rural revitalization and the coordinated development of urban and rural areas is very difficult. It is urgent to strengthen rural ideological and moral construction. The construction strategy of new ethical relationship in rural society in the new era was put forward.

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The foam production and control in submerged liquid fermentation of microbial pesticides

LIU Fang, LIAO Xian-qing, CHEN Wei, ZHOU Rong-hua, WAN Zhong-yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 5-8. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.001

Abstract （413）

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Submerged liquid fermentation has been widely used for the production of of microbial pesticides.The foams are formed during the process of fermentation through the joint effects of aeration,the constituents of media and the secondary metabolites of the mciroorganisms which affect the normal fermentation of microbial pesticides.In this review,it summarized the causes and regularities of foam formation,the adverse consequences of foam and strategies of foam control in the submerged liquid fermentation of microbial pesticides,In order to provide guidance for foam control in submerged liquid fermentation of microbial pesticides.

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Research progress on biotype seed coating agent in China

YANG Dan, ZHEN Jiao-li, LI Fei, HUANG Da-ye, CAO Chun-xia

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 9-12. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.002

Abstract （533）

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Biotype seed coating agent is an environmentally friendly biological pesticide preparation,it is playing an important role in green agriculture of our country.The concept,feature,effective components,function mechanism of biotype seed coating agent were summarized,the development history of biotype seed coating agents in our country was introduced from four aspects of seed disinfection and seedling growth,pest control,seed storage and crop yield,finally,the current problems and prospect of seed coating agent in China were summarized.

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Progress of small molecule compounds targeting DNA ligase IV to improve the efficiency of CRISPR/Cas9 genome editing

XIAO Hong-wei, BI Yan-zhen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 13-19. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.003

Abstract （368）

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Gene-editing-tools can create double-strand breaks (DSB) at specific sites on the DNA chains.There are two different ways to repair DSB in cells,namely homologous recombination (HDR) and non-homologous end joining (NHEJ).NHEJ pathway and HDR pathway compete with each other as alternative DNA repair pathways.DNA ligase IV acts an important role in the NHEJ pathway.Inhibitors of DNA ligase IV can inhibiting the NHEJ efficiency while improving HDR efficiency in the DNA repair pathway.To optimize small-molecule compounds for more effective site-specific insertion of genes,the studies on the four different chemicals SCR7,NU7026,resveratrol and L755507 were reviewed,and these four small-molecule compounds in gene-editing were summarized.Among them,the bioinformatics software was used to simulate the docking of these four chemicals with DNA ligase IV.Based on these,the small molecule compounds used to improving gene editing efficiency was prospected.

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Study on double mechanical transplanting cultivation mode of double cropping rice in southeastern Hubei province

CHEN Jie, XIA Xian-ge, CHEN Zhan-peng, TU Jun-ming, CAO Zhi-gang, ZHANG Qun, JIN Hong-mei, CHENG Jian-ping, ZHANG Zhi-sheng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 20-23. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.004

Abstract （528）

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At present,there are many problems in double-cropping rice planting in southeast Hubei province,such as large labor input,tight stubble,long slow seedling stage and insufficient basic seedlings during mechanical transplanting.Through the research on the annual high yield and high efficiency of double-cropping rice,it was found that the double mechanical transplanting cultivation mode of early rice Liangyou287 and late rice Taiyou398 had high yield,short overall growth period and low production risk,the techniques of double cropping rice scale mechanization and double mechanicaltransplanting were integrated and demonstrated.

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Correlation analysis of agronomic characters and biological yield of summer-sowing food and fodder dual-purpose silage maize

GUO Li, MA Xian-bin, HU Fei, LI Xin-yan, WANG Xi-zhao, LI You-ming

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 24-27. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.005

Abstract （445）

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Through the analysis of correlation and path between agronomic characters and biological yield of summer-sowing food and fodder dual-purpose silage maize (Zea mays L.),the results of showed that the main agronomic traits that were significantly positively correlated with the biological yield of silage maize were plant height,ear height,stem diameter and number of green leaves,among which the correlation value of stem diameter (r=0.7037) was the largest.The correlation coefficient between ratio of whole plant weight to ear weight,ear weight and total fresh weight were positive but not significant.The direct path effects of each character on total fresh weight was in the order of stem diameter >ear weight >ear height >number of green leaves >plant height >ratio of whole plant weight to ear weight,and the direct effects of stem diameter and ear weight on total fresh weight were extremely significant.Stem diameter in the direct path coefficient of total fresh weight was 0.4502,which was the biggest direct positive effect.In order to obtain a higher total fresh weight,it was necessary to select the varieties with thicker stem diameter,higher plant height and ear height,more green leaves and larger ratio of whole plant weight to ear weight.Ear weight in direct path coefficient of total fresh weight was 0.4068,whose direct positive effect value was bigger,but through other characters effect value were negative,so the total fresh weight of silage maize couldnot be determined only by ear weight in character selection,but could be considered increasing the ear weight appropriately.

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Comparative test of fresh corn varieties in Hubei province

ZHANG Xu, QIN Hui-bao, XIONG You-sheng, XIE Yuan-yuan, ZHANG Guo-zhong, LIU Wei, XU Xiang-yu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 28-32. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.006

Abstract （680）

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In order to select high-quality and adaptable fresh corn varieties suitable for planting in Hubei province,a comparative test of fresh corn varieties was carried out in five regions of Hubei province in2019,and the indexes of fresh corn yield,main economic characters,main agronomic characters,growth period and stress resistance were carried out analysis.The results showed that there were significant differences among the five fresh corn varieties.Considering the quality,yield and resistance of fresh corn,Wantian2015 and Xintian477 were recommended to be planted in Hubei province.

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Effects of sowing amount and paclobutrazol on yield characters of wheat Yumai 158

ZHAO Yong-tao, ZHANG Feng, ZHANG Zhong-zhou, YUAN Qian, ZHEN Shi-cong, WANG Gai-ge

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 33-35. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.007

Abstract （493）

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Taking wheat Yumai158 as the research object,the differences in yield and population dynamic characteristics under different sowing rates and spraying amount of paclobutrazol treatments were analyzed.The results showed that under different treatment,Yumai158 had average spike-bearing tiller rate,stable thousand-grain weight,and more grains per spike.The best sowing rate was112.5~150.0 kg/hm²,and the best dosage was600~1200 g/hm² of15% paclobutrazol powder.

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Survey and analysis of crop germplasm resources in Enshi city of Hubei province

LI Li, JIAO Chun-hai, LIU Chang-yan, LIU Liang-jun, WAN Zheng-huang, CHEN Hong-wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 36-43. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.008

Abstract （481）

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Total108 accessions of crop germplasm resources which belonged to46 species of40 genera in24 families were collected in“The3th national action of crop germplasm resources survey and collection”which was carried out in Enshi city.That included13 feeding grain crops,31 vegetables,11 legumes,38 fruit trees,4 tea resources,10 oil crops and1 fiber resource.In this study,local situation,growth and decline conditions of crop germplasm resources were analyzed.Furthermore,the conservation,development and utilization of crop germplasm resources in Enshi city were also discussed.

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Analysis on the suitability of Huaxi district tourism climate

XIA Da-wei, YUAN Xiu, WU Dan, YAN Jiang, AO Deng-ren

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 44-48. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.009

Abstract （437）

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Based on the ground meteorological data of Huaxi district from2007 to2016,the main elements of tourism climate resources in Huaxi district are analyzed,such as temperature,relative humidity,wind speed,precipitation,sunshine,and so on.On this basis,through the temperature and humidity index and wind efficiency index,the annual and monthly climate comfort values of Huaxi district are calculated.The results showed that the climate of Huaxi district is suitable for tourism activities all year round,and it is an ideal tourist destination for tourists,among which April to October is the best tourism period.

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Numerical simulation analysis of a mixed severe convective weather process

SU Tao, YAO Rong, ZHOU Chang-qing, ZHOU Li

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 49-58. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.010

Abstract （383）

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The circulation situation and meteorological features of a strong convection process occurring in Hunan province is analyzed from April3—4,2015,using conventional observation data such as ground and high altitude.The high-resolution WRF model focuses on the strong combination of northeastern Hunan.The convective weather is numerically simulated,and the structure,evolution and trigger mechanism of the strong convective storm under the complex underlying surface of the Dongting lake area are discussed.The results show that this is a mixed strong convective weather in a typical low-level warm-flow forced weather background.The4 hPa up-and-down of ground pressure corresponds to different types of severe convective weather in northeastern Hunan.The continuous strong vertical wind shear and updraft the effect is to maintain the storm,and the convective monomer merges and develops to strengthen the multi-cell strong storm (the supercell storm).The unstable energy accumulation in the northeastern part of Hunan,the local geothermal convection and topography and other uplifting effects,and the low-level flow field sharply merge into a strong rotational rise.The movement triggers the release of unstable energy,which provides favorable dynamic and thermal instability conditions for strong convective weather such as hail,thunderstorm and heavy rain.The simulation results accurately describe the spatial structure and environment of the mixed strong storm in the Dongting lake area.The evolution of parameters has a certain indication for the early warning and forecasting of mixed strong convective weather.

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Analysis of an aircraft artificial precipitation enhancement operation for a typical mixed convective stratiform cloud system in summer and autumn in Hunan

LI Qiong, GAO Qin, TANG Lin, DING Li, WANG Ling, LI Zu-xian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 59-65. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.011

Abstract （353）

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Comprehensive utilization of model,satellite,radar,sounding and other data to analyze the operating conditions of a typical mixed convective stratiform cloud system affected by a tropical low-pressure peripheral cloud system in Hunan.The operation scheme of aircraft artificial precipitation enhancement was designed and carried out.Precipitation enhancement effect was also analyzed through statistical test and intuitionistic physical test.The analysis showed that the weather system configuration of the tropical low-pressure outer cloud system was conducive to the occurrence of precipitation in Hunan.The model index forecast results showed that the cloud water content in southwest of Hunan was sufficient and the supercooled water content was abundant,while the ice crystal number concentration was insufficient,which indicated that there was a good potential for cloud-catalyzed rainfall enhancement.The designed flight operation scheme was basically consistent with the actual flight trajectory.The rationality of the selection of the flight operation area,location and timing selection was verified through the targeted analysis of the comprehensive detection data such as satellite,radar and sounding.The radar echo distribution was relatively uniform before the aircraft rain enhancement operation.During the operation,the radar echo reflectance was significantly enhanced,and the strong echo area and number were significantly increased.The statistical area echo was weakened after the operation.It had a obvious catalytic effect.The rain enhancement rate of this rain process was23.71%,and the amount of increased water was about13.4 million tons.

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Study on boundary layer characteristics of Jinzhou ecological station

ZOU Xu-dong, ZHAO Hu-jia, WANG Xiao-ying, LI Ke-fei, CAI Fu, YANG Hong-bin, ZHANG Yun-hai, WANG Hong-yu, LIU Yu-che

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 66-74. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.012

Abstract （430）

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In order to explore the atmospheric boundary layer structure of Jinzhou ecological station,one day in October2016,February2017,April and July respectively is to be selected.Using NCAR/NCEP data,the characteristics of500 hPa pressure field and850 hPa pressure field weather situation are analyzed;WRF model is used to simulate the surface wind field at8:00 and20:00,the vertical profile wind field,and the48h boundary layer height;Microwave radiometer is used to detect the temperature and humidity of Jinzhou ecological station from the ground to an altitude of10 km.The results show that,the position of Jinzhou is near the trough,the air flow is to the west or north,and the cold air passes through the trough and the temperature drops at the500 hPa pressure field.The position between low pressure system and high pressure system at850 hPa pressure field,the airflow comes from the north or south.The ground wind field is in high pressure divergent air flow,wind direction to west or north.The profile wind fields are all downdraft near the ground.Atmospheric instability occurs at20 o'clock at different altitudes from the ground.There is little change in temperature at a height of10 000 meters from the ground.The height of inversion layer is lowest in February and highest in October.The variation of humidity from the ground to the upper air shows a fluctuating shape.The change is obvious in one day.Among them,there is relatively small change in the humidity within one day of February,the change from ground to upper air is relatively small.The boundary layer height is low between19:00 on the first day and8:00 on the second day.The high values appear between12:00 and15:00.The difference of boundary layer height and low value is small,of which February is the smallest.The difference of the height of the boundary layer is large,with the smallest in February and the largest in July.

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Impact of land use change on ecosystem service value in Lijiang river basin in 1998—2018

HE Yi, TANG Xiang-ling

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 75-80. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.013

Abstract （413）

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Based on3S technology,LANDSET remote sensing images of the Lijiang river basin in1998,2008 and2018 were used to analyze the changes in different regions during the study period through land use change data,and the value of ecosystem services in the Lijiang river basin in1998—2018 was estimated.The results showed that in1998—2018,the forest land had been in a leading position in the Lijiang river watershed land use,land use overall changed mainly for cultivated land to construction land and forest land transfer,including water and arable land area was reduced9.77 km² and415.33 km²,respectively,woodland showed a trend of first increased after decreased and area increased by111.70 km²,construction land and unused land increased significantly,respectively increased by210.34 km² and103.06 km²;The value of ecosystem services in the basin decreased by 0.54% from8 073.8778 million yuan to8 030.1776 million yuan,which was mainly reflected in the trend of first increasing and then decreasing,basically consistent with the change of forest land area.Generally speaking,the spatial distribution showed a trend of high northeast and low southwest.In order of value from high to low,forest land,water area,cultivated land,construction land and unused land were in order.Ecosystem service value of low area appeared expanding trend,and sustained growth,mainly distributed in Guilin city such as downtown,Xing'an,Lingchuan,Yangshuo,median area were mainly cultivated land,gradually occupied by urban sprawl,some high value area was weak increase trend,mainly concentrated in the eastern and northern forest area.

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The effects of future climate change on soybean growth period and yield in Henan province under RCP scenario

LIU Jia, LI Cong, LI Tong-xiao, LIU Yan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 81-85. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.014

Abstract （334）

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Based on the soybean observational data and the corresponding climatic data of Linzhou city in2008—2017,Huangfanqu Farm in2006—2017,Zhengyang county in2000—2008 were selected to verify the model of CROPGRO-Soybean,and the model parameters suitable for the southern,central and northern regions of Henan province were found.By introducing the climate scenario data of future typical concentration paths RCP4.5 and RCP8.5,the response of soybean growth period and yield to climate change in the future climate change scenario were analyzed.The results showed that the flowering period and the first seed period of soybean in Henan showed a prolonging trend under the climate conditions of RCP4.5 and RCP8.5 of the future typical concentration paths,and the prolonging days under the climate conditions of RCP8.5 were more than that of RCP4.5.The prolongation of flowering period of soybean in Henan was the central >the north >the south.And the prolongation of first seed period of soybean in Henan was the central >the south >the north.The maturation period of soybean in Henan was similar under the climate conditions of future typical concentration paths RCP4.5 and RCP8.5,the prolongation trend of soybean maturation period showed the north >the central >the south.Soybean yield in Henan province showed a decreasing trend under the climate conditions of RCP4.5 and RCP8.5,and the reduction under the climate conditions of RCP8.5 was higher than that of RCP4.5.The soybean yield in Henan decreased rapidly from south to north,but the trend in the central region was the smallest.

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Screening of soil-derived bacteria decolorizing malachite green and decoloring condition analysis

LIAO Hua-yuan, XIONG Meng-xia, LI Run-cheng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 86-90. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.015

Abstract （328）

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To screen bacteria capable of decolorizing malachite green (MG) from the soil-derived bacteria,as a result,a strain of Enterobacter cloacae (S1-2) had a strong ability to decolorize MG.According to single variable principle,the different conditions that affected the strain S1-2 were characterized by the change in absorbance of the solution before and after MG degradation.The results showed that the strain S1-2 could completely decolorize1100 mg/L MG within36 h,and the inhibitory rate of treated MG on Bacillus amyloliquefaciens and Bacillus parvii decreased by43.98%~48.21%.The analysis of decolorization conditions with300 mg/L MG found that the degradation rate of 0.5%~7.0% inoculation amount of strain S1-2 was91.67%~93.91%;In different pH culture environment,the strain S1-2 decolorized90.02%~94.54% MG at pH6 and pH7;The strain S1-2 decomposed84.61%~94.56% MG,when cultured at20~40 ℃;Under the concentration of6~18 g/L NaCl,the strain S1-2 degraded91.85%~93.77% MG.

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Canada calcium carbonate volcanic salt rock (SRC) improvement of acidified soil and its application in cabbage

ZHOU Fu-zhong, ZHU Xue-zhu, LIU Ren-bo

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 91-96. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.016

Abstract （444）

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Through field experiments and large scale demonstration,the improvement effect of canada calcium carbonate volcanic salt rock (SRC) on acidified soil and its yield increasing effect on cabbage were verified.The product has good effect on improving the acidified soil.The results show that600~1800 kg/hm² can increase the pH of soil by 0.07~0.28 units in the current season.SRC can also reduce the exchangeable aluminum contentin the soil,reduce the aluminum toxicity in the acid soil,increase the cation exchange capacity of the soil,enhance the ability of supplying and maintaining fertilizer,and increase the exchangeable calcium and magnesium content of the soil.It can reduce the content of available iron and availablemanganese in soil,so as to alleviate soil acidification.On the basis of conventional fertilization,the yield of cabbage can be increased by increasing the amount of SRC.The yield of cabbage can be increased by more than5% when1200 kg/hm² is used;The yield of cabbage can be increased by more than10% when1800 kg/hm² is used,and reached a significant level.

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Effects of hydrogen peroxide and calcium hydroxide pretreatment on cellulose, hemicellulose and lignin content in wheat straw

WANG Ze-jia, CHEN Yang, DONG Yuan-yuan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 97-99. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.017

Abstract （542）

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The experiment took wheat straw as the pretreatment object to study the effect of hydrogen peroxide and lime combined pretreatment with different concentration ratio on the content of cellulose,hemicellulose and lignin (three elements) in wheat straw and the effect of different pretreatment time on the content of three elements in wheat straw.The effects of freezing-thawing conditions on the content of three elements in wheat straw were also investigated.Use titration method to determine three content,through research to determine the optimum freeze-thaw combined alkali pretreatment conditions as followsWhen the mass fraction of hydrogen peroxide and calcium hydroxide was 0.4% and 0.2% respectively after two days of freezing at -10 ℃ and melting at100 ℃,the content of tri-element in wheat straw decreased most obviously,including17.5% cellulose,6.8% hemicellulose and7.8% lignin.

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Research on habitat quality of green infrastructure in Yongding district based on InVEST model

YU Ji-wei, ZHU Shen-hai

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 100-103. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.018

Abstract （439）

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The habitat quality of the green infrastructure in Yongding district was evaluated by the InVEST habitat quality model,which based on the land use data of Yongding district in2017 and combined with the spatial analysis function of ArcGIS software.The results showed that the overall green infrastructure in Yongding district was well maintained.However,because of the deeper urbanization,the construction land continues expanding,and the farmland was reclaimed in disorder,resulting in the degradation of habitat seriously and the quality of habitat poorly around the urban area of Yongding district and its rural area nearby.Human activities were the main factor of ecological change.Taking the evaluation and analysis about the change of habitat quality in Yongding district,the purpose was to provide scientific basis for ecological protection,coordination of the contradiction between ecology and development,and prevent the ecological damage of green infrastructure.

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Research on spatial layout optimization of rural residential areas based on MCR model:Taking Pingshan county in Hebei province as an example

SHI Tie-chou, REN Guo-huan, CHEN Yu-qi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 104-110. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.019

Abstract （499）

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Taking Pingshan county of Hebei province as an example,this paper analyzes the current of the spatial distribution of rural residential areas,selects the evaluation system,determines the space division standard and the basic unit,formulates the evaluation index and selects the evaluation model,and uses the MCR model to calculate the minimum cumulative resistance,to summarize the spatial characteristics of rural residential areas.Based on the present situation,this paper puts forward the optimization countermeasures for the spatial layout of rural residential areas in Pingshan county,and determines the optimization direction,and according to suitable area,relatively suitable area,unsuitable area,extremely unsuitable area four kinds of partition,puts forward four optimization models of urban transformation,gradual merger,relocation and restricted development,and looks forward to the future development of rural residential areas.

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Investigation on the distribution of apple snail (Pomacea canaliculata) in the lower reaches of Yangtze river in China

JI Xiao-mei, WANG Ai-xin, FANG Lin-chuan, LI Chang-lin, XU Lin, YANG Shou-kun, LIU Xian-bao, ZHONG Lan, LIU Yi-man

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 111-116. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.020

Abstract （1357）

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The distribution of apple snail (Pomacea canaliculata) in the lower reaches of the Yangtze river,including provinces of Anhui,Jiangxi,Jiangsu and Zhejiang and the city of Shanghai in China,was investigated,and the distribution characteristics,transmission routes and control strategies of apple snail were discussed.The results showed that some areas in the lower reaches of the Yangtze river are high-density distributed with apple snail and the most often invaded sites include ditches,shallow ponds,aquatic plant fields,rivers and lakes.There three main routes of transmission of apple snail to the lower reaches of the Yangtze river,i.e.,direct introduction by man,indirect introduction with aquatic plants and natural spread.In resent years,indirect introduction with aquatic plants has become the most important route of transmission of apple snail.The main control strategies of apple snailwere introduced.It was suggested that the quarantine for movement of aquatic plants to prevent the spread of apple snail with the plants,and the research in integratedcontrol technology should be strengthened.

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Study on the effects of nitrogen filling insecticide on idle coiling machine in cigarette production workshop

DAI Tian-yi, LIU Chao, HAO Wan-li, ZHAO Xu-sheng, LI Yong-wang, LIU Yong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 117-120. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.021

Abstract （345）

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The effects of nitrogen filling on the idle coiling machine in the cigarette production workshop is studied.The double-layer sealing environment of the crimping machine is created by using the film and the aluminum alloy bracket to realize the double-layer isolation and sealing protection of the winding machine.The idle-rolling machine is subjected to nitrogen and oxygen reduction through the nitrogen generator equipment(The oxygen level of the tabernacle is less than2%) for more than40 days to observe the control effect of tobacco beetle(Lasioderma serricorne).The results show that the idle coiling machine in the cigarette production workshop can effectively kill the various tobacco beetles that breed inside the machine by sealing and purging nitrogen.

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Optimization of fermentation process of Bacillus thuringiensis NBIN-863 by using response-surface method

LIU Xiao-yan, MIN Yong, CHEN Wei, LIU Fang, LIAO Xian-qing, ZHOU Rong-hua, RAO Ben

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 121-124. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.022

Abstract （358）

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The study aimed to optimize the fermentation process of Bacillus thuringiensis NBIN-863.The key factors of the media components were investigated by using Plackett-Burman (PB) design.The central axises of these factors were determined by using steepest-ascent method.The optimized medium for Bacillus thuringiensis NBIN-863 was determined by central combination design and response surface method.It demonstrated that the key factors in NBIN-863 fermentation broth were starch and soybean meal.The optimized medium was obtained through response surface analysis as35.2 g/L of starch,34.7 g/L of soybean meal.The crystal conte nt of NBIN-863 fermentation broth was measured as5.4 mg/mL.The fatality rate of NBIN-863 fermentation broth for nematodes was81.0%,which was very close to the predicted value (81.2%) and80% higher than the actual mortality rate (45.0%) of NBIN-863 before optimization.

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Study on appropriate flight parameters of disease and pest control for plant protection UAV in regenerated rice

YU Wei-pu, ZHOU Zhong-xiong, XU Guo-hua, WU Yun-zi, LIU Mian, XU Hui, YANG Hong-yong, XIE Lei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 125-127. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.023

Abstract （344）

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Based on the common unmanned aerial vehicle (UAV),the appropriate flight parameters of UAV for the control of diseases and insect pests in the ratooning rice field were studied,on the basis of widely used parameter such as flight height,spray volume and droplet size and the control effect of plant protection UAV on diseases and pests under different flight speeds was compared.The results showed that the flight parameters of the UAV represented by Dajiang MS-G1 were set at1.8 m altitude and 0.379 L/min rated flow,which can achieve good control effect in the range of4~6 m/s flight speed.However,with the increase of flying speed,the control effect decreased.According to the comprehensive control efficiency and cost,the control efficiency of the treatment with a flight speed of5 m/s was better than that of other treatments.

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Acute toxicology studies of technical concentrate of Bacillus vallismortis NBIF-001

SHI Li-qiao, LIU Ying-juan, WANG Yue-ying, MIN Yong, LIU Xiao-yan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 128-129. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.024

Abstract （389）

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In order to evaluate the acute toxicity of technical concentrate of Bacillus vallismortis NBIF-001,acute oral toxicity test,dermal toxicity test and inhalation toxicity test were conducted according to the toxicological test methods for pesticides registration (GB/T15670—2017).The results showed that the acute oral LD₅₀ of the biopesticide was more than5 000 mg∕kg in male and female rats,the acute dermal LD₅₀ was more than5 000 mg∕kg and the acute inhalation LD₅₀ was more than5 000 mg∕m³.The acute toxicity of technical concentrate of Bacillus vallismortis NBIF-001 was very low.

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Study on the application effects of a special fertilizer for tea tree in young tea garden

SONG Jing-jing, JIANG Shao-bo

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 130-132. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.025

Abstract （403）

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The effects of Chabaobao special tea tree fertilizer on growth and development of young tea plants and soil were studied in two consecutive years.The results showed that,compared with the conventional potassium sulfate compound fertilizer and urea treatment,the germination time,germination time,first bud first leaf spread and first bud two leaf initial spreading time of the three different quantities of tea tree special fertilizer treatment were2~3 days early,and the survival rate of the first year tea seedlings increased by19.37%,20.48% and21.22%,respectively.The survival rate of the second year tea seedlings increased by23.72%,25.28% and26.61%,and the number of branches increased by16.63%,24.71% and30.48% in the first year respectively.The number of branches of the tea seedlings increased by17.17%,23.23%,26.57% respectively.The height andthe stem diameter of the tea seedlings increased obviously,and the content of soil organic matter and alkali nitrogen could be increased,soil fertilizer and soil were improved.

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A famous variety of purple Caitai——Hongshan Caitai

NIE Qi-jun, LI Jin-quan, DONG Bin-feng, HU Zhi-wei, REN Zhi-yong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 133-135. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.026

Abstract （537）

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The cultivation history,growth environment,characteristics,development and utilization,and cultivation techniques of Hongshan Caitai(Brassica rapa ssp.Chinensis var.purpurea) are introduced.Daguzi,the main variety of Hongshan Caitai,is good quality,it is about90~120 days from sowing to harvesting.The plant height is70~80 cm and the plant width is80~90 cm.The surface of the bolt is purplish red,with wax powder.The base of the bolt is thick,the average diameter of the base of the bolt is2.5 cm,the maximum diameter is more than5 cm.The length of the bolt is35~50 cm,and the maximum weight of the single bolt is more than100 g,the average weight is60~80 g.

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Effects of chemical fertilizer reduction on the yield and quality of spring tea

WANG Hong-juan, WANG You-ping, MAO Ying-xin, HUANG Dan-juan, CHEN Xun, TAN Rong-rong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 136-138. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.027

Abstract （341）

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In order to clarify the effects of fertilizer reduction on the yield and quality of spring tea,four fertilization treatments,including100% fertilizer (local traditional fertilizer amount)A,100% organic fertilizer B,30% organic fertilizer+70% fertilizer C and blank control CK were set.The results showed that the yield,hundred-bud weight and germination density of all fertilization treatments were higher than that blank control,but the differences between different treatments were not significant.The contents of tea polyphenols,amino acids and the ratio of phenol to ammonia in chemical fertilizer reducation treatment were not significantly different from those of other fertilization treatments,and the content of water extract was lower than that of other treatments.The content of catechin(C) in spring tea under fertilizer reduction treatment was significantly reduced,and the contents of C,GCG and CG were different from that under fertilizer treatment.Fertilization could increase the content of chlorophyll a and carotenoid and decrease the content of chlorophyll b,but there was no significant difference between fertilizer treatments.

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Comparative of activity of antioxidant enzymes from Aloe barbadensis Mill. and Aloe vera L.var Chinesis

WANG Bin, ZHAO Jin-rong, ZHANG Teng-xiao, ZHAO Hong-bo, GUO Yan-yao, QI Qi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 139-141. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.028

Abstract （645）

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Using the old,middle and tender leaves,the whole leaves and the gel in the middle part of the leaves of Aloe barbadensis Mill.and Aloe vera L.var Chinesis as sample,the activity of antioxidant enzymes in different parts of the two barbados aloe varieties were compared.The results showed that there were great difference in the activity of antioxidant enzymes in different parts of Aloe barbadensis Mill.and Aloe vera L.var Chinesis.The highest activity of SOD,CAT and POD were in the gel of the old leaves,the middle leaves and the old leaves of Aloe barbadensis Mill.,respectively.The highest activity of SOD,CAT and POD were in gel of tender leaves,the old leaves,the old leaves of Aloe vera L.var Chinesis.Combined with biological production,the gel of middle leaves in Aloe barbadensis Mill and Aloe vera L.var Chinesis should be used as raw materials from the point of view of developing SOD,the the whole old leaves in Aloe barbadensis Mill.and Aloe vera L.var Chinesis should be used as raw materials from the point of view of developing CAT and POD.

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Acute toxic effects of three common fish drugs on Odontobutis potamophila juveniles

LIU Yi-ran, HUANG Yang-yang, WU Qin, XIE De-bing, REN Yan, LI Bo, WANG Hui, ZHU Si-hua, XU Hong-liang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 142-145. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.029

Abstract （788）

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In order to study toxic effect of several common fish drugs on Odontobutis potamophila juveniles,static bioassays were conducted.Results showed that the24,48 and72 h ρ(LC₅₀) of10% povidonum-iodum (PVP-I) to the fish with95% confidence interval was estimated to be1466,1148,997.6 mg/L,respectively,with the safe concentration being270.0 mg/L.Results show that the24,48 and72 h ρ(LC₅₀) of40% phoxim to the fish with95% confidence interval was estimated to be4.030,1.207,0.409 mg/L,respectively,with the safe concentration being 0.1085 mg/L.Results showed that the24,48 and72 h ρ(LC₅₀) of4.5% Beta-thermometric (beta-CYP) to the fish with95% confidence interval was estimated to be3.007,2.695,0.974 mg/L respectively,with the safe concentration being 0.7246 mg/L.

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Effect of cow dung degradation products on aquaculture water quality

LI Pei, CHEN Jian, LIU Wu, LI Qing, WANG Gui-ying, SUN Yan-hong, JIN Er-guang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 146-150. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.030

Abstract （367）

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Simulation experiment was conducted to study the effects of cow dung degradation products on aquaculture water quality at different water depths and fertilization amount.The results showed that the effect of cow dung degradation products on water quality was mainly reflected in the effects on total phosphorus,soluble phosphorus and dissolved oxygen,but not on total nitrogen,ammonia nitrogen.The concentration of total phosphorus and soluble phosphorus in water was positively correlated with the amount of fertilizer,and negatively correlated with water depth,and there was a significant interaction between the two factors.

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Melatonin inhibits zearalenone-induced apoptosis of porcine ovarian granulosa cells

ZHOU Jia-wei, WU Jun-jing, QIAO Mu, WANG Xiao-zhong, LIU Gui-sheng, MEI Shu-qi, PENG Xian-wen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 151-154. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.031

Abstract （352）

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Primary cultured porcine ovarian granulosa cells were used as a model to explore the effect of ZEA on the apoptosis of porcine ovarian granulosa cells,and to determine whether melatonin (MT) regulated the effect of ZEA on the apoptosis of porcine ovarian granulosa cells.The results showed that3 and10 μmol/L ZEA had no significant effect on the apoptosis of porcine ovarian granulosa cells,but30,50 and100 μmol/L ZEA significantly promoted the apoptosis of porcine ovarian granulosa cells,and showed a dose-effect relationship;It was found that 0.1 and1 μmol/L MT partially inhibited30 μmol/L ZEA-induced apoptosis of porcine ovarian granulosa cells and increased cell viability.

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Study on the interaction of porcine APOA2 promoter and transcription factor GATA1

ZHANG Feng, LI Xin, CHEN Kun, CHEN Ming-xin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 155-159. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.032

Abstract （379）

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This study was aimed to clone the potential promoter region of porcine APOA2 gene and analyze its transcriptional activity,obtain its core promoter region,and analyze the effect of transcription factor GATA1 on the transcriptional activity of APOA2 gene for further studying the function of APOA2 gene in meat traits.The results showed that APOA2 gene was mainly expressed in liver and adipose tissue of white pigs.The results of dual luciferase activity indicated that there was the core promoter region of APOA2 gene5 'flanking region at -813 to -565 bp,and there were more likely to be some cis-acting elements promoting APOA2 transcription in the region of -659 to -565 bp.The results of GATA1 over-expression indicated that transcription factor GATA1 promoted the transcription of APOA2 gene.Site-directed mutagenesis assay confirmed that transcription factor GATA1 could directly bind to GATA1 binding sites (-608 to -599 bp) in the promoter region of APOA2 gene.Taken together,these results suggested that transcription factor GATA1 could directly target the promoter region of APOA2 gene and promoted APOA2 transcription.

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Study on compositions and antibacterial activities of petroleum ether extracts from Fritillaria hupehensis Hsiao et k.c.Hsia

FANG Shi-qi, LI Yu, HUANG Dong-hai, AI Lun-qiang, LIU Cui-jun, HE Mei-jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 160-165. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.033

Abstract （358）

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Study on compositions and antibacterial activities of petroleum ether extracts from Fritillaria hupehensis Hsiao et k.c.Hsia,Fritillaria hupehensis Hsiao et k.c.Hsia were collected from Enshi area,Hubei province,China,whoes total extract was obtained by ultrasonic extraction with ethanol solution (60%).And the total extract to obtain petroleum ether segment were extracted.The antibacterial activity and mechanism of petroleum ether segment of Fritillaria hupehensis were studied by means of filter paper method,dilution pour plate method,ultra micro ultraviolet spectrophotometer and conductivity meter.The composition of petroleum ether segment from Fritillaria hupehensis was studied by means of GC-MS,1D and2D NMR.Results showed that Hubei Fritillaria petroleum ether solution was moderately inhibitory sensitive on Bacillus thuringiensis and Pseudomonas aeruginosa,and mildly inhibitory sensitive on Klebsiella pneumonia ATCC13883,Staphylococcus aureus ATCC29213,and Bacillus subtilis.Among them,the MIC/MBC of Bacillus thuringiensis,Bacillus subtilis,and Pseudomonas aeruginosa were less than4.0 mg/mL.Through the exploration of antibacterial mechanism,the petroleum ether section of Fritillaria fragrans Hubei probably slowed the growth of Bacillus thuringiensis,Bacillus subtilis,Pseudomonas aeruginosa by destroying the cell membrane,and finally achieved antibacterial effect.This study also explored the composition of the petroleum ether Extracts from Fritillaria hupehensis Hsiao et k.c.Hsia.Total ion chromatograms of the petroleum ether Extracts from Fritillaria hupehensis Hsiao et k.c.Hsia were obtained and compared with the NIST08.LIB standard library based on retention time and mass spectrometry data.Finally27 compounds of the total ion chromatogram were obtained.

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Fracture analysis of rear axle gear of rice transplanter

LU Wei-an, FENG Tian-yu, HAO Wen-bin, CHENG Jie, HUANG Shi-ming, GONG Rui, WANG Ben-yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 166-169. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.034

Abstract （365）

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To solve the fracture problem of the20CrMnTi gear on the rear axle of the rice transplanter head,the broken gear is inspected for material quality,microstructure observation,hardness gradient detection,metal inclusion detection,and gear meshing dynamic analysis.The structural design analysis was carried out,and the internal relationship between the failure mechanism and strength of carburizing gear was established.The improvement measures of gears in design and technology were put forward.The results showed that the gear fracture is a tooth fracture,the gear is overloaded and the machining tool marks are the main reason of gear fracture.According to the analysis results and corresponding improvement measures were given to avoid similar accidents in the actual production process.

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Tourism image perception of health town based on network text and IPA model:Take Aishan SPA town as an example

GAO Shu-min, ZHANG Jun-xia, CUI Jing-fang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 170-175. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.035

Abstract （476）

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The study on tourism image perception of health town is of great significance to improve the quality of the town and improve the tourists' satisfaction.Based on the travel notes and comments of Ctrip,Meituan,Dianping,etc.,this study systematically analyzed the tourism image perception of Aishan SPA town by using the network text analysis method and IPA model.It was found that the tourism image perception of Aishan SPA town mainly included five aspectshealth care resources,health care environment,health care experience,infrastructure and professional services.Tourists pay less attention to the transportation facilities,accommodation facilities and other hard facilities,but pay more attention to the internal environment,professional services,hot spring resources and other soft environment.On this basis,the relevant suggestions were put forward.

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Effects of topography on farmers' employment:An empirical analysis based on CHIP data

FU Hong-xue, FU Xue-jiao, MIN Yuan-yuan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 176-182. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.036

Abstract （343）

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Based on the location data of4821 poverty villages in Hubei province,this research studied the spatial distribution topography characteristics of poverty villages by using GIS technology.At the same time,by using the data of China's household income survey (CHIP),this research used SUR regression method to test the impact of topography on farmers' employment.The results showed that,40.16% of the poverty villages were located on the flat land with good topography conditions of cultivated land;the topography advantage was conducive to the transformation of farmers' employment;the possibility of farmers' unemployment and the number of unemployed households also decreased due to the topography advantage.

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Game analysis between local government and landless farmers in the process of urbanization

LI Wei-li

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 183-186. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.037

Abstract （401）

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With the acceleration of urbanization,the problems of interest division between local government and landless farmers were becoming more and more prominent.The conflict between them had become an important factor hindering the process of urbanization.Based on the analysis of the game fit point between the local government and the landless farmers,this study constructed the model of the relationship between the two,and explained the results.Finally,it put forward the path to realize the balance of interests from two aspectsthe consistency of the goal and the establishment of the system guarantee.

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Research on the influence mechanism of international crude oil price on China's agricultural products price

GU Jia-le, MENG Qing-jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 187-192. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.038

Abstract （399）

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With the gradual improvement of China's opening to the outside world,the price of China's agricultural products was increasingly affected by the price of international crude oil.Three mechanisms by which international crude oil price affect China's agricultural products price were analyzed.VAR models of international crude oil price and China's overall agricultural products price,soybean price and rapeseed price were established,and impulse response analysis and variance decomposition analysis were performed.The results showed that changes in international crude oil price would have the same effect on China's agricultural products price in the short term,but would have the negative effect after a period of time.At the same time,affecting the overall price of agricultural products by affecting the price of biofuels was an important way for international crude oil price to affect China's agricultural products price.Finally,it put forward suggestions on how to stabilize the price of China's agricultural products.

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Research on the mechanism of urbanization level on energy consumption structure

LI, Xiao-jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 193-198. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.039

Abstract （345）

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Based on the panel data of30 provinces in China from2004 to2016,this study used SPSS software to conduct K-means cluster analysis on urbanization level and energy consumption structure,and empirically test the mechanism of urbanization level on energy consumption structure by using spatial Durbin model.The results showed that,the level of population urbanization and land urbanization had the significant positive effect on the optimization of energy consumption structure;the energy endowment had the certain degree of inhibition on the transformation of energy consumption structure due to the dependence of energy endowment on energy.

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Analysis on farmers' fallow willingness and influencing factors in Urumqi county

FAN Wen-hui, MENG Mei, WANG Zhi-qiang, LI Jia-ning, ZHANG Ming-zhu, GONG Hui

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 199-203. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.040

Abstract （372）

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Cultivated land fallow is an important measure to realize the strategy of “Storing grain in land,Storing grain in technology”,and it is also a necessary way to realize the quality and safety of cultivated land.As the direct participants of fallow,the correct guidance of farmers to actively participate in the fallow was conducive to the steady progress of the fallow policy.Based on the questionnaire survey of313 households in Urumqi county,this study analyzed the farmers' fallow willingness and its influencing factors by using binary logistic regression model.The results showed that age,education level,farmers' support for the fallow policy,and farmers' satisfaction with the fallow compensation had significant positive effects on farmers' fallow willingness,while cultivated land area,cultivated land quality,and the expected subsidy value of farmers had significant negative effects on farmers' fallow willingness.Finally,according to the analysis of the implementation of the fallow policy,put forward relevant countermeasures and suggestions.

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Research on sustainable development path of seabuckthorn engineering forest in Qinghe county under resource constraints

SONG Wei, CHENG Ping

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 204-208. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.041

Abstract （486）

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This study defined the connotation of sustainable development of engineering forest construction,and constructed the analysis framework of sustainable development of engineering forest construction.Taking the seabuckthorn engineering forest in Qinghe county of Xinjiang as the typical case,the development stage and basic situation of seabuckthorn engineering forest were systematically analyzed,and the problems existing in the sustainable development of seabuckthorn engineering forest were examined from the macro and micro levels,the opportunities and challenges of sustainable development of engineering forest in desert oasis were analyzed,which had certain reference significance for the construction of engineering forest in desert oasis.

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Research on the green development efficiency driven by scientific and technological innovation in the Yangtze River Economic Belt

ZHU Dong-yuan, SUN Hao-ran, JI Lei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 209-215. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.042

Abstract （420）

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As the green development of Yangtze River Economic Belt has become the national strategy,scientific and technological innovation as the key driving force to promote green development had important research value.This study used the DEA-SBM model to explore the status and trends of the green development efficiency of the Yangtze River Economic Belt.Based on the panel data of11 provincial administrative regions in the Yangtze River Economic Belt from2011 to2017,the empirical analysis was carried out with the help of spatial econometric model.The results showed that,the overall level of green development efficiency of the Yangtze River Economic Belt was not high,but it had a trend of gradual improvement,and the green development efficiency of the downstream areas was obviously stronger than that of the middle areas and upper areas;the innovation capital and innovation achievements of scientific and technological had a significant role in promoting the green development efficiency,and there was a spatial spillover effect,while the scientific and technological innovation talents had a positive effect on the green development efficiency but there was no significant effect.Finally,put forward the countermeasures and suggestions to promote the regional green development.

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Evaluation and influencing factors of rural tourism development in Henan province

YUAN Meng, WEI Yan, YAN Si-yu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 216-220. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.043

Abstract （541）

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From the five dimensions of resource endowment,natural environment,economic basis,social conditions and tourist market,the evaluation index system of rural tourism development level in Henan province was constructed.Using analytic hierarchy process,TOPSIS method and k-means clustering algorithm,the rural tourism development level in18 cities of Henan province was measured.The results showed that,the overall level of rural tourism development in Henan province was not high,the development was not balanced,and the spatial differences were large.On this basis,suggestions were put forward to speed up the development of rural tourism in Henan province.

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Study on accommodation experience market of rural tourism

SHEN Hui-hui, ZHANG Jie, ZHAO Jin-lin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 221-223. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.044

Abstract （300）

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Through expounded rural tourism hotel industry development condition and experiential economy market mechanism,analysised the relationship between experience landscape and profitable market,it is suggested that we should promote various characteristics of home stay facility and create experience accommodation landscape,focus on consumers' recreation experience,vigorously develop cultural tourism,improve the accommodation environment of rural tourism,in order to promoted sustainable development of rural tourism market.

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Analysis of the institutional innovation of the villagers' self-governance organization: Based on the confusion of its identity

DING ZHI-chun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 224-228. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.045

Abstract （350）

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Villagers' self-governance is an activity that the villagers directly manage the community affairs.The villagers' self-governance organization is the main platform and support for the villagers to exercise their autonomy.At present,there were many grass-roots organizations in rural areas of China.Their legal status was not clear,but they undertake more or less functions of villagers' self- governance.The long-term existence of this situation would not only damage the development of the villagers' self-governance organization,but also bring harm to the construction of the rural governance system.Finally,it would damage the rights and interests of villagers' autonomy.Therefore,it was necessary to clearly define the identity of the villagers' self-governance organization in law,and made corresponding system guarantee and innovationed for the construction and developmented of the villagers' self-governance organization.

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Research on the transformation and development of rural nursing homes for the elderly from the perspective of holistic governance:Take Qidong city of Jiangsu province as an example

DAI Ling-yan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (22): 229-232. DOI: 10.14088/j.cnki.issn0439-8114.2020.22.046

Abstract （308）

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As the main carrier to solve the problems of the elderly in rural areas,rural nursing homes for the elderly played an important role in providing for the elderly in rural areas.From the perspective of holistic governance,taking Qidong city of Jiangsu province as an example,this study analyzed the problems existing in the process of transformation and development of rural nursing homes,such as the fragmentation of management subject's rights and responsibilities,the fragmentation of pension service supply,and the fragmentation of funding sources,and put forward the governance path of building coordination mechanism,improving integration mechanism and establishing trust mechanism.

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Recent advance of alkaloids in genus Aconitum

YANG Shun-yi, YUAN Chun-hong, JIANG Gao-hua, CHEN Ye-gao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 5-10. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.001

Abstract （483）

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In order to study the chemical constituents and biological activities of Aconitum alkaloids,recent advance of alkaloids in Aconitum plants were understood by consulting the literature about Aconitum plants.The result showed that since 2016,scholars had studied Aconitum and found 57 new alkaloids.The study of biological activity showed that some species and alkaloids had significant activities in anti-viral,anti-inflammatory,anti-tumor,anti-cholinesterase,cardiovascular and anti-TMV activities.It is of great significance to study the alkaloids of Aconitum for the development of new bioactive components and resource protection.

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Research progress on selenium enriched fermentation of Lactic acid bacteria

ZHU Jun-yan, LI Huan-mei, WANG Yan-ni, LONG Tong, GAO Wa, ZHAO Xiao, ZHAO Jin-fang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 11-14. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.002

Abstract （567）

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As a kind of element enriched probiotics,many lactic acid bacteria(LAB) can absorb inorganic selenium and convert it into organic selenium and elemental selenium.Selenium enriched lactic acid bacteria have outstanding antioxidant,antibacterial,anticancer and antimutagenic properties.The research status of liquid selenium enriched fermentation of different lactic acid bacteria was reviewed,and the selenium enrichment ability of various lactic acid bacteria was analyzed,which provided theoretical support for the breeding and development of selenium enriched lactic acid bacteria.

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Research progress on development and utilization of Broussonetia papyrifera

LI Yang, SHI Mei-rong, SHU Wen-bo, HAN Lei, ZHEN Bo

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 15-19. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.003

Abstract （651）

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Broussonetia papyrifera is a multi-purpose tree species with good medicinal value,economic value and high resistance.In order to understand and utilize the resources of Broussonetia papyrifera more deeply and to realize the better development and popularization of Broussonetia papyrifera,the growth characteristics,genetic evolution,breeding methods,resistance mechanism and gene function of Broussonetia papyrifera were comprehensively analyzed and the development characteristics and potential were also discussed in order to provide a theoretical basis for the development of Broussonetia papyrifera resources and the breeding of improved varieties in China.

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The latest research progress on porcine pseudorabies

XIANG Min, XIA Yu, WANG Ding-fa, SHAO Zhi-yong, LI Zhong-xin, RUAN Zheng, ZHANG Si-hua, LIU Kai-wu, WAN Xue-ji, LI Xin-yu, SHI Yong-mei, CHENG Lei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 20-23. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.004

Abstract （522）

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:Pseudorabies (PR) infection of pig is an acute contact infectious disease caused by Pseudorabies virus (PRV).The current situation of PR in China,including epidemiology,diagnostic assays,control strategies and challenges of the disease was reviewed in order to provide reference for the prevention,control and eradication of PR in China.

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Developing restorer line pyramiding different resistant genes to blast and brown planthopper by molecular marker-assisted selection in rice

LI Jin-bo, DU Xue-shu, XIA Ming-yuan, WAN Bing-liang, QI Hua-xiong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 24-27. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.005

Abstract （430）

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R022 was used as the receptor parent and GD7 was used as the donor parent to pyramid 4 resistance genes Pi1,Pi2,Bph14 and Bph15 into R650 by molecular marker-assisted selection (MAS).Resistance identification demonstrated that R650 performed resistance to rice blast and brown planthopper.Jufeng 2A/R650,Tianfeng A/R650 and Neixiang 5A/R50,the three hybrid combinations with R650 as restorer line and three sterile lines,performed moderately resistance or moderately susceptible to rice blast and moderately resistance to brown planthopper.Field trial data demonstrated that yields of these three hybrid combinations were higher than or equal to the control hybrid combinations of Jufeng 2A/R022,Tianfeng A/R022,Neixiang 5A/R022 with R022 as recurrent parent under natural field conditions.The combination Jufengyou 650 (Jufeng 2A/R650) was authorized by Hubei Crop Variety Examination and Approving Committee in 2019.

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Study on the cultivation yield and economic benefit of water-saving and drought-resistant rice Hanyou 73 as ratooning rice

WEN Jü, YU Wei-pu, XIE Lei, YANG Hong-yong, LIU Mian, WU Yun-zi, XU Hui, XU Guo-hua

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 28-31. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.006

Abstract （907）

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Compared with Fengliangyouxiang No.1,the water-saving and drought resistant rice Hanyou 73 was cultivated as ratoon rice,and the difference of yield and economic benefit under dry and water pipe conditions was compared.The results showed that the yield of Hanyou 73 in the first season was significantly higher than that of Fengliangyouxiang No.1 in the dry management condition,while the yield in the regeneration season was significantly lower than that of Fengliangyouxiang No.1,and the total yield was the same,consistent with the conclusion under water pipe condition.In terms of economic benefits,Hanyou 73 was slightly lower than Fengliangyouxiang No.1 under dry management condition,and water pipe condition.There is no significant difference between the two.In conclusion,Hanyou 73 can be used as ratoon rice variety in Jianghan Plain,especially in arid climate.

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Discussion on suitable transplanting seedling age and sowing date of “four special” late Indica rice series combinations

LI Xing-hua, CAI Xing-xing, WANG Huan, ZHANG Sheng, ZHOU Qiang, ZHOU Nan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 32-37. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.007

Abstract （503）

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The “four special”late indica rice series combinations has short heading date,poor elasticity of seedling age,and easy to early spike when the seedling age was long,so the yield advantage was difficult to give full play.In order to prevent early ear of “four special”late Indica rice series combinations in production,experiments were carried out with different seedling age and sowing date using three “four special”late Indica rice combinations.The results showed that the highest yield was found in the treatment with 20-day-old seedling age.The most effective panicles per unit area was found in the treatment with 15-day-old seedling age,which was not significantly different with 20-day-old seedling age and 25-day-old seedling age,but significantly higher than 35-day-old seedling age(P<0.05).The seed setting rate and 1 000-grain weight had no significant difference between 30-day-old seedling age and 35-day-old seedling age,but most were significantly lower than 15-day-old seedling age and 20-day-old seedling age(P<0.05).The early ear had already occurred when the seedling age reached 25 days,and was serious when the seedling age more than 30 days.The suitable transplanting seedling age was 15 to 25 days and the most suitable transplanting seedling age was 20 days for “four special”late Indica rice series combinations.According to the safe full-ear and maturity date and the accumulated temperature required for growth period,the sowing of “four special”late Indica rice series combinations could be delayed until June 30 to shorten the seedling age and minimize the effect of seedling age on yield.

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Some characters of water-saving and drought-resistant rice combinations under water and dry cultivation conditions

ZHOU Qiang, WANG Huan, LI Xing-hua, ZHANG Sheng, CAI Xing-xing, ZHOU Nan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 38-40. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.008

Abstract （488）

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A field experiment with Hanyou 804,Hanyou 649,Hanyou 650 and Hanyou 813 as materials was conducted in 2019,the control was Hanyou 73 and Fengliangyou No.4 under water and dry cultivation conditions,respectively.Some characters of water-saving and drought-resistant rice (Oryza sativa L.) combinations under water and dry cultivation conditions were observed.The results showed that the yield of water-saving and drought-tolerant rice increased by 4.86% to 36.60% and -1.47% to 3.51% compared with control (Hanyou73 and Fengliangyou No.4) under dry cultivation and water condition,respectively.Compared with water cultivation condition,chalky grain rate of water-saving and drought-tolerant rice was decreased significantly under dry cultivation condition,but amylose content was increased.In addition,the whole growth period of water-saving and drought-tolerant rice under dry cultivation condition was about 10 days longer than that of water cultivation condition.

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Seedling quality and grain yield of rice to optimized seedling raising methods

LI Zhi-xiang, TANG Ji-chao, WU Zhao-hui, HOU Wei, WU Shan, LU Bi-lin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 41-45. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.009

Abstract （508）

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To solve the problems of soil acquisition difficulties,low quality of seedlings,w eak root twisting power,late greening and slow growth after transplanting in the industrialized rice seedling raising of the rice-wheat rotation area in North Central Hubei Province,Specifically,three different seedling raising methods were tested to study the effect of optimized seedling raising methods on seedling quality and grain yield:bast fiber mulching film combined with nutrient soil for seedling raising and mechanical transplanting (T1),bast fiber mulching film combined with organic matrix and chemical control for seedling raising and mechanical transplanting (T2) andbast fiber mulching film combined with organic matrix and nutrient soil mixed for seedling raising and mechanical transplanting (T3).Nutrient soil for seedling raising and mechanical transplanting (CK1) and water cultivation of seedling and artificial transplanting (CK2) were used as the controls.The results showed that compared with CK1,CK2 and T1 treatments,the seedlings quality of T2 and T3 treatments were higher,the tillering speed and the dry matter accumulation in the early growth stage after transplanting were faster,the leaf area index and SPAD value in each growth period were larger,andits effective panicle number,seed setting rate and grain yield increase.The grain yield of T2 and T3 treatments increased by 6.70%~6.95% compared to CK1 treatment,reaching a significant level;and increased by 4.90%~5.14% compared to CK2 treatment,reaching a significant level.The results indicated that the seedling raising methods of T2 and T3 can improve seedling quality and increase grain yield.

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Simplified cultivation techniques and green prevention control of high-grade rice variety Huangkexiang No.5

CHEN Jie, CHEN Zhan-peng, TU Jun-ming, CAI Zheng-jun, CAO Zhi-gang, ZHANG Qun, JIN Hong-mei, ZHANG Wen-chao, LI Cheng-fang, HUA Hong-xia, CAI Wan-lun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 46-47. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.010

Abstract （548）

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Huangkexiang No.5 is a cultivated high-grade rice variety breed by Huanggang Academy of Agricultural Sciences,which is improved the resistance to blast and high quality conventional of Yuzhenxiang.The cultivation techniques and green prevention and control were studied,to tap the potential of increasing yield,improve the quality of rice and in crease the planting efficiency,and to provide the technical support for the demonstration and promotion of the key technologies of green and low-carbon direct seeding rice cultivation and the cultivation mode of cost-saving,high-yield and high-efficiency.

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Effects of planting density and nitrogen application on yield and related characters of direct-seeded cotton variety Gangmian 11

ZHAO Shu-qi, YAN Zhen-hua, ZHANG Hua-chong, LI Wei, HUANG Xiao-li, DAI Bao-sheng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 48-50. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.011

Abstract （405）

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In order to provide theoretical support for the popularization and application of direct-seeded cotton Gangmian 11,three densities and five nitrogen fertilizer treatments were used to study the effects of density and nitrogen application on the yield and related characters in eastern Hubei province.The results showed that in a certain range,with the increasing of nitrogen application,the yield of Gangmian 11 increased.When the nitrogen application was higher than 112.5 kg/hm²,the yield was lower than that under this nitrogen application level.when the high density treatment was 82 500 plants/hm²,the yield was the highest,and there was no significant difference between the high density treatment and the medium density treatment of 71 250 plants/hm².To sum up,in eastern Hubei province,to obtain high yield,the density was controlled at 72 000 plants/hm² to 82 000 plants/hm² and the nitrogen application could be reduced to 112.5 kg/hm².

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Effect of no nitrogen application on growth and yield of different broom corn millet varieties

LUO SHi-wu, CHENG Bing-wen, YANG Jun-xue, WANG Yong, ZHANG Shang-pei, LI Kai

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 51-54. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.012

Abstract （369）

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Eight broom corn millet (Panicum millaceum L.) varieties were treated with no nitrogen and normal nitrogen application,in order to study the effects of low nitrogen stress on dry matter above ground,root volume,chlorophyll content (SPAD) in leaves and yield of different broom corn millet varieties.The results showed that no nitrogen stress had effects on dry matter above ground,root volume and yield of different broom corn millet varieties during growth,and the differences were obvious between treatments,and the nitrogen use efficiency of different broom corn millet was also different.Among them,the yield of Yanshu No.8 had lower tolerance to low nitrogen stress index,and higher contribution rate of nitrogen fertilizer to yield,indicating that this variety has higher dependence on nitrogen,and more sensitive to low nitrogen stress.The yield of Ningmi 14 had higher tolerance to low nitrogen stress index,and higher contribution rate of nitrogen fertilizer to yield,indicating that this variety has less dependence on nitrogen and stronger tolerance to low nitrogen.

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Effects of paclobutrazol at different concentrations on economic traits and yield of rape

LIU Nian, TANG Tian-ze, ZHONG Si-cheng, LI Bin, FAN Qi-xin, MENG Da-qing, LI Ying-chun, LI Zhi-fan, CHEN Jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 55-57. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.013

Abstract （466）

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Using Guohaoyou No.8 as a test material,the effect of spraying paclobutrazol at different concentrations during the rape (Brassica napus L.) bud period was studied.The results showed that rapeseed could not only cultivate strong seedlings,improve plant type structure and yield characteristics,but also effectively solve the problem of rape lodging when spraying paclobutrazolin.The number of carob fruits per plant,the number of grains per grain,the weight of the rapeseed,and yield of Guohaoyou No.8 was increased by spraying with 1 500 grams of 15% paclobutrazol wettable powder per hectare during the bud period.

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The effect of flooded treatment on the enrichment of arsenic in rice

YANG Yang, GAO Li-li, ZHANG Hui, LYU Xiao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 58-62. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.014

Abstract （451）

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The arsenic(As) concentration in soil and As enrichment in rice (Oryza sativa L.)were studied under different flooded treatment using pot experiments.The results showed that As in soil solution mainly existed as As(III) and its concentration increased with flooded time for flooded treatment in whole rice growing (F).The As concentration was low and mainly existed as As(V) for unflooded treatment in whole rice growing(NF).When soil were aerobic and flooded for FNF and NFF treatments after flowering,As in soil solution decreased and increased markedly,respectively.At the stem extension,weight of rice was not affected by different flooded treatment.But As concentration was much lower in unflooded rice than in flooded rice.Grain weight in the different flooded treatment followed the decreased order of NFF,F,NF and FNF.As concentrations in grain,straw and empth grain followed the decreased order of F,FNF,NFF,NF.While the order of As concentrations in rice tissues from highest to lowest were straw,empth grain and grain.

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Analysis on water environment protection of historic and cultural towns and villages in north China: A case of ancient Guangfu town, Hebei province

ZHANG Da, LI Jia-le, DU Jin-jin, ZHANG Guang-yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 63-67. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.015

Abstract （488）

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Complete protection of water environment of historical and cultural villages and towns is an important issue for the protection and utilization of cultural heritage.By collecting water sample from peripheral ponds and moats,pools and residents' drinking water in typical ancient Guangfu town in north China,and taking advantage of grey relational appraise method,it found that the water environment of ancient Guangfu town was in line with the characteristics of that in Chinese traditional ancient towns and villages.Sanitary sewage,livestock wastewater and runoff and drainage of farmland as well as travelling of tourists were main contaminative sources.Water supply and drainage infrastructure was imperfect and point source pollution was the main form of pollution.In addition,tourists were also one of the factors affecting the water environment of the ancient town.According to the results of analysis,the thesis thus initially proposes measures were put forword to solve water environmental problems.The above research provides relevant basis for the proper protection of water environment of historical and cultural villages,thus to pave the way for improvement of the quality of the connotation of ancient Guangfu town and construction of beautiful country.

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Collaborative control of water pollution of river basin based on four dimensions

ZHANG Zhu-ye, LIU Zhong-lan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 68-72. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.016

Abstract （408）

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Water resources have typical characteristics of quasi-public and externality.However,because water resources in a river basin are divided by different administrative regions,regional conflicts of interest and disputes over pollution control are easy to occur in their jurisdiction and governance,thus hindering the process of water pollution control in the river basin.Therefore,how to establish a collaborative governance mechanism,coordinate the interest relations and conflicts among the main bodies,and compensate the adverse effects brought by the division of public property of water resources and administrative system is of great significance for the water pollution control in the river basin.Based on the existing theoretical basis,this paper analyzes the practical reasons for the dilemma of water pollution control in the river basin,including the defects of the governance system and mechanism as well as the unsound legal system.It is concluded that a new model of collaborative governance should be established,including system collaboration,power collaboration,platform collaboration and consciousness collaboration.

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Temporal and spatial variation analysis of soil salinization in Yinbei irrigation area of Ningxia from 1989 to 2019

WU Xia, WANG Zhang-jun, FAN Li-qin, LI Lei, ZHANG Yong-hong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 73-76. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.017

Abstract （428）

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Yinbei irrigation area of Ningxia was choosed as the research object and remote sensing image was taken as the data source.Based on the classification maps of soil salinization types in Yinbei region in 1989,1999,2009 and 2019,the spatial statistical analysis method was used to analyze the evolution characteristics of soil salinization in the study area.The results showed that,from the perspective of time change,the salinized cultivated land in Yinbei region showed an increasing trend from 1989 to2019,with a large increase form 1989 to 2009 and a small increase from 2009 to 2019;From the perspective of spatial evolution,the degree of salinization gradually transits from centralized distribution to fragmented distribution;From the perspective of transfer matrix,the transformation of soil salinization types in Yinbei region from 1989 to 2019 was relatively large for the complexity,the transfer area between non salinized cultivated land and light to moderate salinized cultivated land was large.

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The dynamic prediction for rapeseed yield based on influence index for bumper or poor harvest from historic meteorological yield in Sichuan basin area

ZHAO Yi, ZOU Yu-jia, ZHANG Yu-fang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 77-80. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.018

Abstract （442）

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As one of nationʼs big producers of rapeseed(Brassica napus L.),timely and accurate yield forecasts has great significance to ensure national grain and oil security in Sichuan province.In order to determine the type of historically similar years,the rape yield data and daily meteorological data from 1983 to 2012 at 30 stations in Sichuan basin area,as well as growth period of 13 agricultural meteorological observatory data were used to set up a comprehensive diagnostic index which based on correlation coefficient and Euclidean distance.By analyzing the relationship between meteorological influence index of rape yield in forecast year and similar year in history,the dynamic prediction method of rape yield in Sichuan basin area was determined.The results showed that the average accuracy of dynamic trend prediction was equal or above 80%,and the average accuracy of basin integration was equal or above 70%.The average precision of dynamic quantitative prediction in each producing area of Sichuan basin was equal or over 89.37%,and the average precision of basin integration was equal or over 96.16%.The precision of dynamic quantitative prediction in each producing area of Sichuan basin was equal or over 89.11%,and the precision of basin integration was equal or over 95.84%.The forecast results indicate that the method has good performance of accuracy to apply and can be applied to actual service.

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Prediction model of tobacco leaf sensory comfort based on chemical composition

YAN Tie-jun, CHEN Si-meng, ZHOU Hong-shen, XU Zi-cheng, PANG Zhe, LIU Wen-feng, PAN Ting-ting, SHAO HUI-fang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 81-86. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.019

Abstract （425）

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To improve the effectiveness of evaluation of cigarette sensory quality comfort,170 standard samples of flue-cured tobacco in China's main tobacco-producing areas in 2019 were selected as materials.Based on the conventional chemical components and polyphenol compounds in the tobacco leaves,the descriptive statistics and simple correlation analysis were first used to analyze the chemical components.Then,the principal component analysis method was used to screen the chemical components,and then the stepwise regression analysis method was used to establish a model for the prediction of cigarette sensory comfort.Results showed that,compared with the real value,the prediction result obtained by the model had smaller error,and the result was relatively stable.Which indicate that the prediction model of tobacco sensory comfort established by principal component-stepwise regression analysis method has high accuracy and feasibility.

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Influence of different nutrient solution formulations on hydroponic Syngonium podoopphyllum cv. White Butterfly

GUO Cong, GUAN Wei, ZENG Xiang-guo, HAN Yong-chao, ZHANG Qing-hua, WANG Jian-rong, XIANG Fa-yun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 87-93. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.020

Abstract （526）

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In order to explore the formula of large and medium nutrient solution suitable for hydroponic Syngonium podophyllum cv.White Butterfly,five common plant hydroponic nutrient solution formulations and one self-modulated formula were selected in the experiment.With clear water as the control,the growth status of S.podophyllum cv.White Butterfly and its hydroponic solution changes were observed and recorded under seven treatments.Then the formula proportion of large and medium amount of nutrient solution suitable for hydroponic S.podophyllum cv.White Butterfly was selected.The results showed that hydroponic culture could effectively promote the growth of S.podophyllum cv.White Butterfly.The addition of nutrient formula had little obvious effect on the root elongation and plant height growth,but had significant effect on other growth indexes.The self-modulated formula could promote the lateral root growth,the leaf growth and the increase of plant width.The Yamazaki hydroponic mitsuba formula could effectively promote the extraction of adventitious roots and the increase of crown width.The SCAU leaf vegetable B formula,the Yamazaki hydroponic mitsuba formula and the self-modulated formula could maintain and promote the new leaf development rate.However,the modified Hoagland formula,the Japan Enshi formula and the general formula for foliage plants were not suitable for the hydroponic S.podophyllum cv.Write Butterfly due to the unsatisfactory effect on plant root system and leaf spreading.Although the excellent nutrient solutions can promote the growth of hydroponic S.podophyllum cv.Write Butterfly,the pH value and electrical conductivity of these nutrient solutions in use still need to be further optimized and adjusted in the later stage to maintain the stability of the solution.

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Comparison test of greenhouse cultivation of seven bitter gourd varieties

JIANG Yan-yan, CHEN Zhan-peng, YAN Liang, GE Chang-jun, XU Li-rong, DAN Jun-fen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 94-96. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.021

Abstract （559）

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The growth period,growth potential,fruit characters and yield of seven bitter gourd(Momordica charantia L.) varieties were compared in greenhouse.The results showed that the comprehensive characters of Qingfengwang and Changqing were better,which could be used as early maturing varieties in greenhouse of Huanggang suburban vegetable base;Duruanlianggua-1 and Heikugua-1 can enrich residents' vegetable basket as later autumn varieties,and the relevant cultivation techniques need further study.

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Study on technique of modeling Forsythia suspensa as mantis

WANG De-fang, ZHENG Zhi-yong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 97-100. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.022

Abstract （477）

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Selecting Forsythia suspensa as the test material and mantis as the creative theme,Paratenodera sinensis Saussure,female imago of Chinese Broadsword Mantis common in China was chosen as bionic object.Catching the habitual movement before hunting,the bionic art design of the external form of trees was carried out around the features.Shrubs that have been grown for four years were selected for directional planting in the center of the mold,and the image modeling was made by pruning and binding process.Then interesting garden landscape was built with green sculpture always presented as vivid mantis by careful maintenance.

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Research report on lotus industry development in Hubei province

LI Feng, ZHOU Xiong-xiang, KE Wei-dong, HUANG Xin-fang, ZHU Hong-lian, ZHONG Lan, ZONG Yi-xiang, WU Man, PENG Jing, LI Shuang-mei, YUAN Tian-yao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 101-106. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.023

Abstract （502）

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In Hubei province,lotus(Nelumbo nucifera Gaertn.) is mainly cultivated in the wetland of Jianghan plain and its surrounding low hills,with a planting area of over 96 300 hm² and a primary product yield of 1.7 million tons,with an output value of nearly 6 billion RMB in 2019;The processing capacity of lotus product is about 250 000 tons,and the output value is about 5 billion RMB;New cultivars,new cultivation techniques,new cultivation patterns and new equipments of lotus have been applied in production areas with remarkable economic benefits;The management main bodies of the lotus have grown stronger and played a leading role in promoting the integrated development of three industries of lotus.There are some problems in lotus industry,such as lack of high quality and disease-resistant cultivars,low seedling quality,large amount of chemical fertilizer,shortage of labor,lag in intensive processing and insufficient exploration of lotus cultural value,etc.It is suggested to strengthen the work in policy support,scientific and technological innovation,intensive processing,brand building and integration of the three industries.

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Screening of extreme germplasms with stone cell content traits in pear

FAN Jing, CHEN Qi-liang, YANG Xiao-ping, ZHANG Jing-guo, HU Hong-ju

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 107-109. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.024

Abstract （402）

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In order to strengthen the exploitation and utilization of excellent characters in pear germplasms,the stone cell content of 853 pear flesh was detected and analyzed.The results showed that,the stone cell content was 0.043%~4.764%,the average value was 0.812%,the coefficient of variation was 68.72%,the median value was 0.694%.17 extremely low germplasm resources with stone cell content less than 0.1% and 10 extremely high germplasm resources with stone cell content more than 2.5% were screened out.These specific resources could be used in pear breeding,genetic population construction and lignin metabolism research,so as to improve the utilization efficiency of germplasm resources.

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Study on mechanism of Arisaema consanguineum anti-cancer based on network pharmacology

HAO Ai-ping, WU Qi-kang, GUO Hui-yan, XUE Ju-kun, WEI Ji-cheng, REN Ru-yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 110-115. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.025

Abstract （406）

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This study used the method of network pharmacology to study the anticancer mechanism of Arisaema consanguineum.The active components and targets of Arisaema consanguineum were searched using the TCMSP database,and these targets were uniformly named using the Uniprot database.Cytoscape 3.7.1 was used to establish a compound-anti-cancer target visualization network map,and the STRING database was used to construct the PPI network.Enrichment pathway analysis was performed using the DAVID website and the ImageGP database.Bioprocess analysis was performed using ClueGo software.The results showed that,a total of 42 anti-cancer active components and 65 anti-cancer targets were collected.The main anticancer active substances of Arisaema consanguineum include terpineol and linoleic acid.Important anti-cancer targets are ADH1B,ADH1C and the like.Arisaema consanguineum can show the multi-component,multi-target and multi-pathway anticancer function by by regulating TNF signaling pathways and p53 signaling pathways through biological processes such as participation in neuroactive ligand-receptor interactions and cancer processes.

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Analysis on ruminal environment and rumen bacterial diversity of grazing Dongbao blackhead goats in grass withering period

ZHANG Nian, YANG Qian-ping, TAO Hu, XIONG Qi, LI Xiao-feng, CHEN Ming-xin, SUO Xiao-jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 116-121. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.026

Abstract （500）

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The aim of this experiment was to study ruminal environment and rumen bacterial diversity of grazing Dongbao blackhead goats in grass withering period.The forge grass were collected and the common nutrition contents were determined.Six healthy annual grazing Dongbao blackhead goats (3 males and 3 females) with the body weight of (45.24±1.25) kg were selected and marked using ear tags with numbers,and the rumen fluids of them were collected via oral cavity before free-range with empty stomach in grass withering period to measured the ruminal environment parameters and rumen microbial diversity.The results showed that the pH of rumen fluid of Dongbao blackhead goats was 6.65,the concentration of ammonia nitrogen (NH₃-N) was 50.30 mg/L,and the ratio of acetic acid/propionic acid was 4.43.A total of 290 868 valid 16S rDNA sequences and 479.67 operational taxonomic unit (OTU) across 6 samples were obtained.The richness indexes Ace and Chao1 indexes of ruminal bacterial were 533.79 and 523.31,respectively,and alpha diversity indexes Shannon and Simpson indexes were 6.39 and 0.96,respectively.The rumen bacteria of grazing Dongbao blackhead goats belonging to 15 phyla,23 classes,29 orders,34 families and 87 genera were identified via taxonomic summary.Among these rumen bacterial populations,the dominant bacterial phyla were Bacteroidetes (75.75%) and Firmicutes (15.62%).The dominant bacterial families were Rikenellaceae (45.89%),Prevotellaceae (19.18%).Rikenellaceae_RC9_gut_group (45.64%) and Prevotella_1 (15.46%) were the dominant bacterial genera.In conclusion,the rumen bacterial diversity of grazing Dongbao blackhead goats was low in grass withering period,and the ratio of acetic acid/propionic acid was high.Bacteroidetes is the predominant phylum and Firmicutes is the second most abundant phylum.Rikenellaceae_RC9_gut_group is the most abundant genus in the rumen bacterial community.

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Study on the body size and carcass traits of the Jianghan chicken,recessive white feather broiler and the progeny of their hybrid

SHEN Jie, HUANG Tao, WU Yan, PI Jin-song, PAN Ai-luan, LIANG Zhen-hua, SUN Jing, ZHANG Hao, PU Yue-jin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 122-125. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.027

Abstract （491）

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In order to meet the diversification of consumer tastes,the breeding enterprises gradually focus on the identification of the characteristics of Chinese indigenous chicken which could be used for genetic improvement with commercial strains.In this study,a hybrid experiment was designed to explore the body growth weights gain,body size and carcass traits of the progeny of reciprocal cross of the recessive white feather broiler and the jianghan chicken.The results showed that compared to the jianghan chicken,the growth weights gain of offsprings derived from jianghan chicken as father was obvious increased.Besides,the body size and carcass traits of the offsprings were ranged in the Jianghan chicken and recessive white broilers,and the modes of the reciprocal cross could significantly affect the body size and carcass traits of the cocks rather than hens.

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Breeding of Jianghan chicken A strain

PAN Ai-luan, WU Yan, SHEN Jie, DU Jin-ping, CAI Chuan-peng, PI Jin-song, ZHANG Hao, HUANG Tao, PU Yue-jin, DUAN Jun, HUANG Zhu-lin, SUN Jing, LIANG Zhen-hua

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 126-129. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.028

Abstract （355）

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The breeding of Jianghan chicken A strain was studied for 4 generations by modern breeding methods for whole family and MAS,with Jianghan chicken as the basic breeding material,high-yield genes were introduced.The results showed that the feather characteristics of Jianghan chicken A strain were basically stable,with 100% of may-feather chicken.For the core group of generation 4,the age of 50% of egg production was 7 d in advance;Body weight at age of 300 days was improved by 139.10 g and 90.81 g for cock and hen,respectively;The average egg weight of generation 0 was 51.26 g,and that of generation 4 was 50.28 g.The egg production number of generation 4 was 104.35,increased by 9 compared with generation 0;The proportion of green-shell eggs in the observation groups of generation 3~4 was over 99%,and it was 100% in core groups for generation 0~4.The proportion of green-shell eggs can be precisely controlled by MAS in commercial stocks.

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Effect of epigallocatechin gallate on lipid deposition and liver anti-oxidative capacity in heat-stressed broilers

LI Zhen-jun, WU Han, YANG Ye

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 130-133. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.029

Abstract （355）

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This study aims to investigate the effects of EGCG on the lipid deposition and liver anti-oxidative capacity of broilers under heat stress.One hundred and ninety-two 2-wk-old broilers were divided into four groups with 6 replicates per group and 8 chickens per replicate.One thermoneutral control group (28°C,TN group),which was fed the basal diet;And three cyclic high-temperature groups (35°C from 7:00 to 19:00;28°C from 19:00 to 7:00,heat stress (HS) group),which were fed the basal diet with added EGCG at doses of 0 mg/kg (HS0 group),300 mg/kg (HS300 group),and 600 mg/kg (HS600 group),respectively.The growth performance and liver metabolism and lipid deposition indices were performed at 35 d of age.The results showed that heat stress significantly reduced body weight,daily weight gain,daily feed intake,and decreased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px),and the Nrf2 mRNA expression in liver,and increase significantly the levels of malondialdehyde (MDA) and the expression of LITAF,NF-κB,FAS,SREBP1 mRNA and the lipid deposition compared with TN group.EGCG (HS300 and HS600 group) increased the activities of SOD and GSH-Px and catalase (CAT),and increased the Nrf2 mRNA expression,and decreased the MAD contents,and reduced the lipid deposition and expression of LITAF,NF-κB,FAS,SREBP1 mRNA.The results of this study show that EGCG can improve liver antioxidative capacity to alleviate oxidative damage caused by heat stress.

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Analysis on the safety of livestock manure applied to the field in Hubei province

RAN Zhi-ping, LIU Ming, TAO Li-wen, WEN Jiang-hui, ZHOU Qi, DENG Bing, GAO Qi-shuang, PENG Xia, TAN Jun-jun, SHAO Zhong-bao, PU Zhen-yu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 134-138. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.030

Abstract （451）

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In order to promote the security of manure application to field,this study estimated the suitable area of cropland needed by manure application,total nitrogen and phosphorus load of cropland and the potential of using manure from growth of main crops in Hubei province.The results showed that,planting rice called for smallest cropland area for reusing manure.In the other word,rice owned the strongest capacity to take manure nitrogen and phosphorus away from field.From the perspective of regulation of fecal pollution,the manure nitrogen and phosphorus load per unit area of arable land in Huanggang city were maximum in Hubei province,and they had surpassed the EU′s standard of limitation on nitrogen and phosphorus which were 170,35 kg/hm²,respectively.Closely followed by Ezhou and Xiangyang city,which should be focused on.From the potential of manure application to field,less space was left for manure application in Shennongjia Forestry District,Huanggang city and Enshi city.Therefore,the total amount of livestock and the proportion of manure applied to cropland should be controlled in these regions.

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The technology of producing and optimizing biochemical fulvic acid utilizing straw

GAO Xi-long, HAN Meng-qi, HUANG Ming-hui, ZHANG Qian-ru, LI Hua, LUO Xing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 139-142. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.031

Abstract （460）

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Using crop straw as raw materials,biochemical fulvic acid can be produced by fermentation method,and the fermentation process was optimized to provide theoretical basis for the utilization of straw with high added value.The results showed that the yield of biochemical fulvic acid was higher when the mass ratio of wheat straw to peanut straw was 2∶1.The content of biochemical fulvic acid could reach 95.10 g/kg after fermentation at 35 ℃ for 216 h.The addition of urea and fermentation bacteria were beneficial to the increase of fulvic acid yield,but the effect was not obvious when urea was added more than 2% and fermentation bacteria was added more than 3%.Wheat straw and peanut straw were mixed according to the mass ratio of 2∶1,adding 2% urea and 3% bacterial agent at 35 ℃,and fermentation for 216 h had better preparation effect of biochemical fulvic acid.

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Evaluation of uncertainty in determination of acesulfame in pastry by UPLC-MS/MS

MO Li-yan, TANG Ming-hua

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 143-147. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.032

Abstract （359）

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Analyze the whole process of determination of acesulfame in cakes by UPLC-MS/MS method,confirm the source and size of uncertainty,synthesize the uncertainty,calculate the expanded uncertainty and report the results.The content of acesulfame was 0.940 9 mg/kg,and its expanded uncertainty was 0.39 mg/kg.Through the analysis of the source of uncertainty and the quantitative evaluation of each component,the preparation process of the standard working solution is the main source of uncertainty.

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Establishment of rapid identification system for inferior oil

LIU Ying-sha, HE Xin-quan, WANG Sheng-wu, LU Yao, XU Yan-yan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 148-151. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.033

Abstract （325）

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In this experiment,11 kinds of oil samples from different sources were tested and analyzed in 9 ways,including sensory evaluation,water content,acid value,peroxide value and plant oil mixed with animal oil,mineral oil,hemp oil,tung oil and aflatoxin.A rapid identification system for inferior oil (gutter oil) was established and suggestions were provided to consumers.

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A multi-food image recognition system based on candidate region

SHEN Feng-xian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 152-155. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.034

Abstract （437）

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This paper proposes a method for recognizing multi-food images by detecting foods in candidate regions and classifying them according to different characteristics.The candidate areas are detected by fusing the output results of multiple area detectors to combine the image feature MKL of each category to estimate the optimal weight.The food recognition method based on feature fusion was applied to the bounding box of candidate regions with various visual features,and through experiments,arranged in descending order of score to estimate images of multiple candidate foods.The results show that for a variety of food image data sets,a classification rate of 55.8% is achieved.The research results have certain reference significance for the field of similar food identification.

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Study on tissue culture and rapid propagation of Zingiber officinale Roscoe cv. Fengtoujiang

YE Xing-zhi, CHENG Qun, ZHANG Deng-hong, XU Yi, CHEN Qiao-ling, CHEN Huo-yun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 156-158. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.035

Abstract （368）

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To screen out the optimal culture medium and build the virus-free rapid propagation system for Fengtoujiang,experiment was carried out with tissue culture technology,using stem apex of as explant and different phytohormone combination.The results indicated that the optimal effect on survival of the callus and stem apex was the combination of MS+6-BA(1.5 mg/L)+NAA(0.1 mg/L).The formation of callus was induced by the medium of MS+6-BA(2.0 mg/L)+KT(0.1 mg/L)and the robust adventitious bud was increased as well.Not only had the MS+6-BA(1.0 mg/L)+NAA(0.3 mg/L) the most efficient effect on ginger root differentiation,but also improved tillering and stand establishment,which increased the propagation significantly.

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The expression of exogenous genes in mammalian cells by using baculovirus

ZOU Gui-lian, LUO Qing-ping, SHAO Hua-bin, WEN Guo-yuan, YANG Ye, SHANG Yu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 159-163. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.036

Abstract （381）

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Baculovirus is a kind of virus that infects invertebrates,and they are widely used in scientific research and biomedicine as expression vector and gene transduction vector.Based on “Bac-to-Bac”operating system of baculovirus,we transformed baculovirus vector into a transduction vector with capable of expressing exogenous genes in mammalian cells in this study.First,the PH and p10 promoters of pFastBac-Dual plasmids in the Bac-to-Bac operating system were replaced with the CMV promoter and EF1 promoter.Then,the egfp gene and mCherry gene were inserted into the downstream of EF1 promoter and CMV promoter respectively.The transposition was performed to obtain the recombinant baculovirus genomeexpressing egfp and mCherry genes,and recombinant baculovirus was obtained by transfect the genome to Sf9 cells.The recombinant baculovirus can generate green and red fluorescence in insect cells and mammalian cells,indicating that the recombinant baculovirus constructed with CMV promoter and EF1 promoter can express exogenous genes in insect cells as well as mammalian cells through transduction.This vector can be used for eukaryotic expression of special proteins and research and development of gene therapy drugs.

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Establishment of tissue culture and rapid propagation technology system of Angelica keiskei Koidzumi

YANG Li-ping, SHI Qun, CHEN Li-wen, YANG Qiong, LI Fang-fei, CHENG Su

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 164-168. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.037

Abstract （357）

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Taking the Angelica keiskei Koidzumi as test materials,through setting up different treatments,the optimal treatment methods and medium formulations of explants disinfection,axillary bud induction,proliferation culture,rooting culture and seedling transplanting were studied,and the tissue culture and rapid propagation technology system of the plant was established.The results showed that using 0.1% HgCl₂ disinfection for 10 min can achieved the ideal disinfection effect,the contamination rate was controlled at 13.3%,the germination rate was up to 83.3%,and the effective buds were up to 96.0%.The optimal culture medium for bud induction wasMS+0.80 mg/L 6-BA+0.10 mg/L NAA,and the buds began to germinate in about 4~6 days.The most suitable culture medium for subculture was MS+0.80 mg/L 6-BA+0.10 mg/L NAA+0.20 mg/L AD,and the coefficient of proliferation was 3.39.The rooting effect of single use of IBA or NAA is better than the combination of the two.When NAA concentration is 0.40 mg/L,the rooting rate reached 100% when cultured for 10 days,and the rooting number and average root length are better than other treatment combinations.The yellow-soil was selected as the transplanting substrate,and the seedlings with complete root system were transplanted.The survival rate was over 98% after 20 days.

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Identification of different transcripts of porcine MMP14 in PAM cells and thier bioinformatics analysis

WU Jun-jing, WAN Xu-ling, HUANG Cang-yu, QIAO Mu, WU Hua-yu, MEI Shu-qi, PENG Xian-wen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 169-173. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.038

Abstract （287）

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Matrix metalloproteinases 14 (MMP14) is the first discovered membrane-type matrix metalloproteinases in the matrix metalloproteinases super family.MMP14 protein can hydrolyze various extracellular matrix components and serum proteins,promote extracellular matrix degradation,and hydrolyze cytokines,chemokines and growth factors.In this study,two different porcine MMP14 transcripts were found in pig alveolar macrophages (PAM).The expression levels of different transcripts of MMP14 gene in porcine PAM cells infected with PRRSV were detected by fluorescence quantitative PCR,the results showed that the expression level of transcript NM_214239.2 significantly reduced (P<0.05),while XM_021098303.1 significantly incresed (P<0.01) in PRRSV infected PAMs.Bioinformatics analysis results showed that these two different transcripts encoded two different proteins.Compared with NM_214239.2 encoded protein,the RRKR motif and PG_binding_1 functional region were missing in XM_021098303.1 encoded protein,which might not havefunctionalactivity in the inactivation of MMP14 protein.It is speculated that the inactivation of MMP14 after PRRSV infection maybe relate to the high expression of cytokines and chemokines stimulated by PRRSV.However,the specific role of MMP14 in PRRSV infection still needs further study.

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Analysis of tillering characteristics, related-nitrogen assimilation enzyme activity and gene expression in rice

AN Qi, JI Wen-jun, HE Yong-gang, ZHANG Zhi-hong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 174-179. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.039

Abstract （405）

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Tillering of rice is one of the important agronomic traits that affect the number of panicles and thus the yield of rice.It was found that low-node (early-onset) tillers had higher yield per panicle than high-node (late-onset) tillers.12 rice varieties with different tillering characteristics were used as meterials.Based on the observation of tiller development dynamics under hydroponics conditions,ammonium nitrogen assimilation enzyme activity and rice tillering-related gene expression were analyzed.Correlation analysis found that the number of tillers was significantly positively correlated with the tillering rate in the second and third leaf positions,but no significant correlation between tiller number and tiller rate at the frist leaf position in rice.The tillering rate at the first leaf position was mainly affected by the ammonium assimilation enzyme activity in rice seedlings,while the expression of genes related to the auxin and monopetalactone signaling pathway did not significantly affect it.The second and third leaf tillering rate were relatively weakly affected by the seedling ammonium nitrogen assimilation enzyme activity and gene expression,but was mainly negatively regulated by the expression of genes related to the auxin and strigolactone signaling pathways.Besides,the soluble sugar content of rice seedlings,genes such as MOC1 that control tiller bud formation and genes that control CK synthesis were not significantly correlated with the tillering rate in the first,second and third leaf positions,while genes such as TB1 that regulate tiller bud elongation had a more significant effect on it.

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Research on the emergency management ability of farmers' professional cooperative fund mutual aid emergency based on AHP-entropy fuzzy method

LIU Jing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 180-188. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.040

Abstract （383）

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This study defined the emergency of mutual fund of farmers' professional cooperative,built an index system of 7 first-level indexes and 27 second-level indexes,which took the emergency management ability of the emergency of mutual fund of farmers' professional cooperative as the object of evaluation.AHP and entropymethod were used to determine the weight of each index,and a multi-level fuzzy comprehensive evaluation model was established to comprehensively evaluate the respondents' ability of emergency management of mutual fund emergencies.The empirical analysis shows that the evaluation index system is scientific and effective,and has high application value,which is of great significance to prevent the occurrence of the emergency risk of the mutu al fund emergency.

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Analysis on time, space and driving force of coordinated development of marine and terrestrial economies in coastal provinces and cities of China

MU Ting-wei, HUANG Ling-hai

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 189-196. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.041

Abstract （417）

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The coordinated development of marine and terrestrial economies is of great significance to China's economic and social development.Based on the data of 11 coastal provinces andcities in China from 2006 to 2016,this study constructed an index system from the coupling of sea-land system,selected Super-SBM method to evaluate the the development of land and sea economy,and calculated the coupling coordination degree of sea and landeconomic development,and then analyzed the spatial patterns and driving force of oupling coordination degree by using spatial statistical tools.The results showed that,except Shanghai and Hainan,the promotion of marine system in other provinces and cities to land economy were higher than that of land system to marine economy.The coupling coordination degree of Tianjin,Hebei,Liaoning,Shandong and Guangxi were on the decline,while Liaoning,Zhejiang and Guangdong were in the stage of mild maladjustment.The coupling coordination degree showed negative spatial autocorrelation from 2006 to 2008 and positive spatial autocorrelation from 2009 to 2016.More than 45% of coastal provinces and cities were in the region with low coupling coordination degree.The coupling coordination degree was affected by the coupling coordination degree of neighboring provinces and cities;Economic driving,government regulation,investment driving,market driving,science and technology driving and coupling coordination had positive spillover effects,while environmental inversion and coupling coordination had negative spillover effects.

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Research on land renovation in hollow village:Take Chengcheng county in Shaanxi province as an example

WANG Tong-da, ZHAO Yong-hua, HAN Lei, HUANG Xin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 197-202. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.042

Abstract （432）

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In order to save land resources,promote the dynamic balance of total arable land and sustainable land use,it is very important to carry out the rectification work of the abandoned residential land in the hollow village.Through the research on the hollow village of Chengcheng county,Shaanxi province,the following conclusions were drawn①The main reason for the formation of the hollow village in Chengcheng county was that farmers spontaneously improve housing conditions and large-scale mining of coal resources lead to changes in topography.②The evolution process of hollow village in Chengcheng county was a process from partial hollowing to complete hollowing.③From the perspective of legal system,cultural concept and technical means,the rectification measures of hollow village were clarified.Through the evaluation of the cultivating property,it was detersmined that the hollow village of Chengcheng county should adopt the technology of remediation and rejuvenation,soil rebuilding and fertility enhancement.A comprehensive approach to remediation.④New hollow village planning link,it will be divided into service-oriented towns and development-type towns according to the situation of each township,plan different development routes,actively build the central towns,and realize the development concept of the towns and towns driving the surrounding towns.

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Ecological security assessment in Hubei province based on P-S-R model

YE Sai, JIAN Xue-ying

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 203-207. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.043

Abstract （406）

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In order to effectively assess the ecological security status of Hubei province from 2014 to 2018,10 major cities in Hubei province were selected as the research objects for empirical study.Combined with the ecological characteristics of the urban agglomeration in the middle reaches of the Yangtze River,based on the pressure-state-response model,13 indicators including the natural population growth rate,the comprehensive utilization rate of solid waste and the proportion of the tertiary industry in the regional GDP were selected to construct the ecological security index system of Hubei province.The weight of each indicator was determined by principal component analysis.The comprehensive index method and comprehensive evaluation model were used to evaluate the ecological security status of Hubei province,and based on the evaluation results,suggestions for promoting the high- quality development of Hubei province were put forward.

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Analysis on the subject mechanism of China's ecological environmental damage compensation claim

DING Chen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 208-211. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.044

Abstract （387）

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The determination of the claimant for ecological environmental damage is the basis of the compensation system for ecological environmental damage.Only when the claimant is clarified can the claim process be initiated,the compensation system for ecological environmental damage can be implemented,and the damaged ecological environment can be effectively repaired.At present,China's main body of compensation for ecological and environmental damage is still in the preliminary exploration stage,and there are some problems such as incomplete laws and regulations,a single scope of claim subjects,and unclear division of labor among subjects.Therefore,it is necessary to further improve China's eco-environmental damage compensation claim subject mechanism from the aspects of improving China's relevant laws and regulations,expanding the scope of claims,refining the division of labor,and clarifying the order of claims,based on the analysis of the theory of eco-environmen tal damage compensation claim right,drawing lessons from the relevant provisions of foreign countries.

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Study on status quo and influencing factors of the UEBMI participation by construction industry migrants

WANG Ying, SHANG Yue

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 212-215. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.045

Abstract （424）

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Based on the data of the China migrants dynamic survey in 2017,the Logit regression model was used to study the status quo and influencing factors of the UEBMI participation by construction industry migrants.The study found that older age,less educated,higher percentage of household registration in agriculture,lower long-term intention to live locally and lower average monthly household income,lower percentage of residence in urban communities,lower percentage of employment in public institutions or state-owned enterprises,and lower percentage of permanent employers were the significant characteristics of construction industry migrants without insurance coverage.Regression results further showed that older,male,low level of education,agriculture census register,short distance migration,inter-provincial migration,had no intention to local long-term residence,low family income,living in rural communities,did not work in the institutions or state-owned enterprises,construction workers,no stable employers town worker medical insurance coverage probability was lower.

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Research on the tourism image of Xi'an city based on the analysis of network text

XIANG Jin, Suo Nan Qu Zhen Zhou Ma, ZHAO Di-xian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 216-221. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.046

Abstract （392）

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This study taked the microblog users as the research object,and used the research methods of content analysis,semantic network analysis and kernel density estimation to analyze Xi'an's tourism image from two aspects of evaluative tourism image and structural tourism image,and put forward relevant suggestions.The results show that Hui Ethnic Street,Bell Tower,Drum Towerand City Wall are the core tourism images of Xi'an city.Ruins are the most preferred theme of tourist images.Tourism emotion evaluation is mainly positive.Xi'an's tourism image pattern has formed three major tourism image areasHui Sstreet-Bell and Drum Tower tourism image area with tourism and food as the theme,Dayan Pagoda-Ancient City Wall-Yongxingfang tourism image area with ruins as the theme,and Terracotta Warriors-Huaqing Palace tourism image area with history and culture as the theme.Image elements show the characteristics of centralized center and scattered periphery.The image of hot spots in urban tourism is still centered on material entity image.It not only supplements the limitations of the previous research on the image of urban tourism in terms of methods,but also provides suggestions for the development of urban tourism in the internet era.

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The measurement of urban green development guality in the Yangtze river economic belt and the analysis of its influencing factors

HE Yuan-jing, SU Pan-da, WEN Chao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 222-229. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.047

Abstract （508）

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Based on the panel data of 11 provincial capitals in the Yangtze river economic belt from 2003 to 2017,the entropy method was used to analyze the quality of green development,and Tobit panel regression model was used to analyze the factors affecting the quality of green development.The results show that the quality of green development of cities in the Yangtze river economic belt is fluctuating and rising,and the regional differences are obvious.The overall trend is gradually decreasing from the upstream cities to the downstream cities and then to the middle cities,that is,the trend of “high at both ends and low in the middle”The level of economic development,market opening,foreign investment and environmental regulation play a significant role in improving the quality of green development.However,industrial structure,scientific and technological progress and urbanization play an inhibitory role in the quality of green development.The optimization path of improving the quality of urban green development in the Yangtze river economic belt was put forward from four aspectsDocking national strategy,increasing government support,optimizing industrial structure and coordinating regional differences.

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Dicussion on the ecotourism development model of rural communities-centered

CHEN Jin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 230-232. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.048

Abstract （441）

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In order to realize the sustainable development of eco-tourism,it is necessary for rural communities to take the community as the main body to promote tourism.Based on the literature analysis method combinind with field investigation,this paper first analyzed the connotation of ecotourism,community ecotourism,then analyzed the cause of domestic promoting ecological tourism,and based on this,taking the rural community as the core of the ecological tourism development mode was put forward,in order to further promote the healthy and sustainable development of ecological tourism in China.

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Research on the mode and path of e-commerce poverty alleviation under digital rural strategy:Taking Taihu county in Anhui province as an example

CHEN Qing-yu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 233-237. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.049

Abstract （455）

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In order to provide new ideas for the development of e-commerce poverty alleviation,this paper investigated and studed the current situation of e-commerce poverty alleviation in Taihu county.Through the analysis,it was found that Taihu county created and promoted “e-commerce platform+leading enterprise+base+poor households”and “e-commerce platform offline consumption”e-commerce poverty alleviation mode,and implemented the “logistics+e-commerce”synchronous operation mode.However,there were some problems in infrastructure,object awareness and ability,brand construction and promotion,and high-end e-commerce talent reserve.In view of its experience and problems,it was proposed that e-commerce poverty alleviation should consider different stages of e-commerce development.In the initial stage of e-commerce development,government guidance should be given priority.In the growth stage,characteristic resources should be brought into play.In the mature stage,innovative promotion methods should be adopted.

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Network governance:A logical approach to governance of rural settlement environment from the perspective of rural revitalization

ZHU Li-juan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (23): 238-241. DOI: 10.14088/j.cnki.issn0439-8114.2020.23.050

Abstract （413）

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Since the implementation of the rural revitalization strategy,the environmental governance of rural settlements has aroused the attention of the academic community on environmental governance models.The current single government-led model,market model and peasant participation model are still difficult to solve the complex problems faced by the diverse rural environment in China,and the governance effectiveness is poor.The management of rural collective environment urgently needs to establish a network that applies the relationship between multiple governance entities and the rural environment.Based on the endogenous subject status of the village that coexists with the rural environment and the construction of the “1+n”governance model,the current rural environment governance has many contradictions in terms of information issues,institutional supply,subject status and responsibilities.Through the network model to establish environmental information service platform,the use of endogenous governance subject value guidance,multi-party cooperation and co-governance,and area responsibility bundling and other multiple measures,will be effectively resolved to promote the healthy development of rural environmental governance.

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Research progress on the biological activity and function of plant polysaccharides

ZHANG Qin, LI Mei-dong, LUO Kai, HUANG Xiu-fang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 5-8. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.001

Abstract （549）

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With the development of chemistry, nutrition, immunology and traditional Chinese medicine, people's research on plant polysaccharides continues to deepen. A number of fruitful research results have been obtained, providing a large amount of experimental data and theoretical basis for the study of human health and the biological activity of plant polysaccharides themselves. This paper was mainly to review the biological activity of plant polysaccharides in recent years, in order to provide reference for subsequent research.

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Research on the application of key information infrastructure security strategy

XING Li-ping, CHEN Kan, WANG Fan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 9-15. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.002

Abstract （438）

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On the basis of combing the security problems of key information infrastructure in Hubei province, combining the grade evaluation report to reflect the main problems and suggestions for rectification, and according to the research ideas of constructing security architecture, the technical strategies including strengthening online security product protection strategy, adding redundant network key nodes and seamless access of security products to the current network are adopted to carry out phased rectification step by step. The results show that the application of various techniques solves the problem of missing redundant design of WAN nodes in the key information infrastructure of Hubei province, and alleviates the irregular situation that the login address of core network equipment and server is not uniform, the password complexity and the periodic replacement are difficult to implement. Through the grade evaluation and risk assessment of the evaluation organization, the safety protection grade requirements and standards of the key information infrastructure in Hubei province are gradually related, and the safety protection ability is improved year by year.

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Research progress on the function of ubiquitination modification in DNA damage repair

QIN Xiao-bing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 16-20. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.003

Abstract （418）

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DNA damage can cause instability of genome, therefore, which makes the normal cells cancerous. Therefore, the DNA damage repair is essential for genome stability and preventing cells from becoming cancerous. There are numerous DNA damage repair factors participate in DNA damage repair response in the cells, some of them will be modificated when they functionating in DNA damage repair response, one of the most significant modifications is ubiquitination modification. In this review, we will shed light on the research progress of ubiquitination modification in the DNA damage repair response,and to provide theoretical basis for the treatment and basic research of related diseases correspondingly.

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Breeding and identification of a wide-compatibility line HDH73 in rice

XIA Ming-yuan, LI Jin-bo, QI Hua-xiong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 21-23. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.004

Abstract （453）

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The exploration of heterosis in indica-japonica hybrids is an important approach to increasing the yield potential of rice (Oryza sativa L.). Hybrid sterility was a major obstacle for utilization of such heterosis. The wide-compatibility genes provided valuable genetic resources to effectively solve the problem of hybrid sterility for rice breeding. An intermediate line HDH73, which had good agronomic characteristics and wide compatibility gene, was bred by anther culture with indica-japonica hybrid Yongyou 4949 as donor. The high-throughput SNP (single nucleotide polymorphism) genotyping showed that the indica/japonica classification of HDH73 was partial japonica, and the frequency of japonica loci of HDH73 accounted for about 2/3 by SNP analysis with polymorphism marker between indica and japonica. The hybrids of Guang 3S/HDH73 and Changlijing S/HDH73 were planted and investigated agronomic characteristics. The growth stages and plant heights of these hybrids were similar to those of the medium-indica hybrids which were widely planted, and the seed setting rate was fertile. The rice line HDH73 could be wide-compatibility resource in breeding program for utilization of inter-subspecific heterosis.

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The relationship among phenotype, heterosis and combining ability of hybrid rice

CHEN Jun-xiao, YOU Ai-qing, LIU Kai

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 24-28. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.005

Abstract （659）

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We constructed a NCII population and performed a high-throughput phenotyping facility (HRPF) and combined with artificial investigation to measure all traits, to investigate the relationship between phenotype, general combining ability, special combining ability and heterosis of yield-related traits. The results showed that there were high correlations among phenotype, SCA, GCA and heterotic advantage for all traits. The phenotype was always positively correlated with GCA and SCA, but GCA and SCA contributed variously to different agronomic traits;heterotic advantage always positively correlated with GCA and SCA, and SCA contribute more to the heterosis. GCA always negatively or not correlated with SCA, the correlations with heterosis and parent genetic distance (PGD) and observed heterozygosity (OH) were not particularly evident in our data.

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Effects of different sowing date and planting density on yield and agronomic characters of E-Jing 403

LI Pei-de, YANG Guo-cai, LI Zhen-lian, LIU Kai, CHEN Zhi-jun, ZHOU Lei, XU Hua-shan, ZHA Wen-jun, LI San-he, YOU Ai-qing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 29-31. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.006

Abstract （418）

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To investigate the effects of different sowing date and planting density on yield and agronomic characters of direct-seeding rice after wheat harvest, japonica variety E-Jing 403 was grown in field experiment with 3 sowing dates S1(05-25), S2(06-04), S3(06-14)and 4 densities D1(45 kg/hm²), D2(60 kg/hm²), D3(75 kg/hm²) and D4(90 kg/hm²). The results showed that the sowing date has a great influence on the whole growing period of E-Jing 403. With the postponing of sowing date, the whole growing period is shortened. Direct seeding density had extremely significant effects on basic seedlings, highest seedlings and effective ears,while sowing date had extremely significant effects on plant height, percentage of available tiller and yield. In order to improve the yield of direct seeding rice E-jing 403, the seeding rate should be reduced in early sowing and increased in late sowing.

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Evaluation of high and stable yield ability of wheat varieties in middle and lower reaches of Yangtze river by high-stable yield coefficient method

ZHANG Yu-qing, ZHU Zhan-wang, LIU Yi-ke, CHEN Ling, ZOU Juan, TONG Han-wen, HE Wei-jie, ZHU Guang, LI Xiang-cheng, WANG Wen-xue, GAO Chun-bao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 32-34. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.007

Abstract （460）

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The high and stable yield ability of 26 wheat varieties (lines) in the regional trial of the middle and lower reaches of the Yangtze river in 2016-2017 were analyzed by using the high-stable yield coefficient (HSC) method. The results showed that the evaluation results acquired by HSC method were basically consistent with that of conventional statistical analysis. But HSC can measure the consistency of high and stable yield of varieties more simply and intuitively through HSC value. After analysis, Huihong 225 was evaluated as a typical high and stable yield variety as its yield, HSC value and coefficient of variation ranked the first, the first and the second respectively. So it should have a wide popular prospect in the future.

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Analysis of agronomic characters and quality performances on wheat germplasm of different types planted in humpy ground in northern Hubei province

LIU Ying, JIN Xiao-jie, LING Dong, ZUO Zhen-xi, TAN Jing, LONG Xiao-ling , WEN Liang, WANG Li-feng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 35-40. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.008

Abstract （490）

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The performance and variation of wheat (Triticum aestivum L.) quality and agronomic characters on wheat germplasm of different types planted in humpy ground in Xiangyang of northern Hubei province were studied. We have analyzed the agronomic characters and quality indices. The variation analysis and correlation analysis were made on five agronomic character indices including grain number per spike, 1 000-grain weight, plant height and 11 wheat quality indices such as protein content, wet gluten content and sedimentation value. The results of variation analysis showed that the grain number per spike had the highest variation, while the least variation was found on 1 000-grain weight and spikelet number. As for quality indices, the bulk density was with the smallest variation while stability time had the highest variation. Correlation analysis showed that significant positive correlations among the agronomic characters and quality indices. It should be considered in wheat breeding and germplasm resources innovation to improve the selection efficiency of high quality wheat and the accuracy of comprehensive evaluation.

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Analysis and evaluation on tuber quality of different potato varieties

ZHU Jie, SU Xiu-li, WU Guo-wen, FANG Zhi-guo, WEN Hai-xia, ZHANG Jie, FENG Peng, WANG Si-qing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 41-44. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.009

Abstract （510）

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In order to select new potato varieties with good quality, a set of comprehensive analysis and evaluation methods of potato quality was put forward, and the nutritional quality, processing quality and commodity quality of 6 potato varieties were evaluated. The results showed that Hongmei had the best nutritional quality, Huashu No. 3 had the best processing quality and commodity quality, and Huashu No. 3 was the best in comprehensive quality analysis.

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Study on fertilization effect and appropriate fertilizer application of green manure rapeseed

ZHOU Yu-ting, TIAN Gui-sheng, YOU Jiang-feng, WU Hai-ya, CHEN Yan, YOU Shao-yang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 45-47. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.010

Abstract （454）

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In order to find out the application effect and suitable application amount of fertilizer on green manure, six different fertilization treatments were set up to study the effects of different fertilization dosages on the phenological period, winter solstice and biomass of green manure rapeseed. The results showed that fertilization not only prolonged the growth period of green manure and improved winter solstice, but also had a certain effect on improving the biomass of green manure. Compared with the treatment without fertilization (T₁), the growth period of green-fertilized rapeseed treated with fertilization (T₂ to T₆) was delayed by 2~5 days, and the plant heightincreased by 6.3~9.7 cm, rhizome thicknessincreased by 4.7~5.0 mm, leaf number per plant increased by 1.7~2.8 tablets,and leaf area of winter saplings increased by 21.1~41.3 cm²; Compared with T₁, the biomass fresh weight of vegetated vegetable moss increased by 14 775~31 410, 16 290~33 585 kg/hm², and dry weight of vegetated vegetable moss increased by 9 300~21 990 and 10 140~24 345 kg/hm² respectively at the mature stage and in the fertilized vegetable moss stage. The optimal fertilizer rate of green rape in Wuxue city was N 75 kg/hm², P₂O₅ 30 kg/hm² and K₂O 45 kg/hm².

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Complete experimental analysis of fertilizer effect of waterlogged paddy soil rice “3414” in Zhongxiang city

FENG Jing, LI Jing-rong, GUAN Shao-hua, LI Hong-jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 48-50. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.011

Abstract （456）

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“3414” fertilizer effects test design scheme was adopted to carry out field experiments to explore the optimal amount and ratio of nitrogen, phosphorus and potassium fertilization in waterlogged paddy soil rice. The current recommended amount of nitrogen fertilizer (N 180.0 kg/hm²), amount of phosphorus fertilizer (P₂O₅ 67.5 kg/hm²) and amount of potassium fertilizer (K₂O 90.0 kg/hm²) on rice yield increase was studied. The results showed that fertilization increased the yield significantly, with an average yield increase of 235.1~3 066.6 kg/hm², with growth of 3.5%~45.3%. Fertilization increased the gross income by 587.8~7 666.5 yuan/hm², increased the investment by 886.5~2 361.1 yuan/hm², the yield to investment ratio was 0.7~3.9, and the net income was -298.5~5 697.0 yuan/hm². The agronomic utilization rate of nitrogen fertilizer was 8.7~19.5 kg/kg, 13.5 kg/kg for P₂(P₂O₅ 67.5 kg/hm²) and 4.8 kg for P₃(101.3 kg/hm²), and 6.5 kg/kg for K₂(90.0 kg/hm²).

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Effects of nitrogen application on yield increase, nutrient absorption and nitrogen use efficiency of wheat in Hubei province

ZOU Juan, LI Xiang-cheng, ZHANG Zi-hao, TANG Hao-jun, WANG Peng, YAN Shuang-yi, ZHANG Yu-qing, GAO Chun-bao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 51-55. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.012

Abstract （508）

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32 field experiments were conducted in wheat areas in the hills of central Hubei and highland of north Hubei(CNH) and Jianghan plain(JP) from 2015 to 2018. Three treatments were set, namely CK(no fertilization), N₀PK(no nitrogen application) and NPK(combined application of nitrogen, phosphorus and potassium). The grain yield, stem and leaf biomass of wheat(Triticum aestivum L.) were measured at the maturity stage, and the contents of nitrogen, phosphorus and potassium in each part were analyzed. The results were indicated that application of N fertilizer increased wheat yield significantly, the average grain yield increment resulting from N application was 1 838 kg/hm², and increase rate was 58.2%. N contribution rate to wheat was 36.8%. The effect of yield increasing by N application in CNH was more significant than that in JP. Compared to N₀PK treatment, N, P₂O₅ and K₂O uptakes of NPK treatment at harvest were increased by 72.97%, 56.19% and 83.13%. N recovery efficiencyof wheat in Hubei province was 34.0%, and agronomic N use efficiency, N physiological efficiency of wheat in Hubei province were 10.2 kg/kg and 33.0 kg/kg, respectively. N use efficiencies of wheat in CNH were higher than that in JP. Nutrient absorptions per 100 kg grain in Hubei province averaged 2.87 kg N, 1.02 kg P₂O₅ and 2.51 kg K₂O, respectively. The combination of N with P and K fertilizers could increase the shoot biomass and nutrient uptake significantly, the grain yield of wheat in different region of Hubei province also improved simultaneously. At the same level of wheat yield, N and P₂O₅ absorptions were high in JP, and K₂O was high in CNH.

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Rice fertilizer reduction effect assessment study based on fuzzy hierarchical analysis： Take Hubei province as an example

YANG Li, MA Chao-hong, HU Shi-you, DUAN Jian-she, XU Wei-ming, TU Tao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 56-62. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.013

Abstract （459）

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By constructing a fuzzy comprehensive evaluation model, using the technical, economic, social and environmental indicators, organic fertilizer instead of fertilizer volume, fertilizer application reduction value, the effect of rice fertilizer reduction in rice rotation area of Hubei province was evaluated by 14 three-level indicators, such as the degree of government policy support response and the improvement of soil environment in farmland. The overall effect evaluation was better, among which the technical, economic and social evaluation was better, and the environmental effect evaluation was very good.

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Effects of Brassica campestris L. ssp. chinensis var. utili Tsen et Lee waste returning on yield and nutrient accumulation of kidney bean

XU Da-bing, XIE Yuan-yuan, XIA Xian-ge, ZHOU Jian-xiong, YUAN Jia-fu, ZHAO Shu-jun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 63-66. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.014

Abstract （422）

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Field experiment was carried out to study the effects of Brassica campestris L. ssp. chinensis var. utili Tsen et Lee waste returning and fertilizer reduction on yield on yield and nutrient accumulation of kidney bean. The results showed that compared with conventional fertilization, the yield of kidney bean increased by 13.41% and 29.43% with the half and all Brassica campestris L. ssp. chinensis var. utili Tsen et Lee waste returning and conventional fertilization, respectively. On the basis of returning all the waste of Brassica campestris L. ssp. chinensis var. utili Tsen et Lee, the yield decreased by 25.41% of the treatments with 30% reduction of chemical fertilizer and all waste returning. The biomass increased by 9.38% of the 30% reduction of chemical fertilizer and all waste returning treatments comparing to conventional fertilization. When compared with conventional fertilization, the nitrogen, phosphorus and potassium accumulation increased by 18.72%~32.55%, 6.35%~38.22%, 5.73%~41.87% of waste returning treatments, and the nitrogen and potassium mainly existed in stems, leaves and fruits.

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Analysis of the timing characteristics and influencing factors of agricultural carbon emissions in Hubei province

ZHANG Jin-xin, HU Wan-ling, WANG Hong-ling

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 67-74. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.015

Abstract （461）

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Agricultural carbon emissions in Hubei province from 1993 to 2017 using 6 types of carbon sources was calculated, including fertilizers, pesticides, agricultural films, agricultural diesel, agricultural planting area, and agricultural irrigation area. By analysing of time-series characteristics and LMDI addition and decomposition, the influencing factors of agricultural carbon emissions were studied in Hubei province. The results showed that Hubei's agricultural carbon emissions generally increased first and then declined between 1993 and 2017, reaching its peak in 2012, but with a significant upward trend, and agricultural carbon emissions increased with a significant downward trend. The carbon emissions and increments per unit of GDP had a significant downward trend, the carbon emissions per unit of arable land had a significant upward trend, and the carbon emissions per unit of arable land had a significant downward trend; From the decomposition results of the influencing factors, agricultural production efficiency, industrial structure, rural population and agricultural industrial structure were the main influencing factors of agricultural carbon emission reduction in Hubei province, and their contributions decrease in turn, while the level of economic development and urbanization were the main influencing factors leading to the increase of agricultural carbon emission in Hubei province.

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Content and spatial variability of nitrogen and phosphorus in dich sediment in a typical small area of Jianghan plain

NIE Yu-lian, XIONG Gui-yun, LIU Dong-bi, HUANG Min, FAN Xian-peng, WU Mao-qian, XIA Ying, LIU Yi

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 75-81. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.016

Abstract （371）

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A typical small area naturally enclosed by drainage and irrigation canals was selected to monitor and analyze the content and spatial variability of nitrogen and phosphorus in 0~10 cm sediment. The results showed that in the 36 sediment samples, the total nitrogen content was 0.7~2.9 g/kg, the average value was 1.79 g/kg. The total soluble nitrogen content was 17~166 mg/kg, the proportion of inorganic nitrogen in total soluble nitrogen was 88.1%~94.5%, the main part of inorganic nitrogen was ammonium nitrogen, the proportion of which was 84.7%~90.4%. The total phosphorus content of the ditch sediment was 0.4~1.3 g/kg, which averaged 0.60 g/kg, the Olsen-P was 10.2~131.7 mg/kg, accounting for 2.6%~10.1% of the total phosphorus, and the water-soluble phosphorus content was 0.5%~3.7% of Olsen-P. The total nitrogen, total soluble nitrogen and ammonium nitrogen, as well as total phosphorus, Olsen-P and water-soluble phosphorus in the ditch sediment showed a very significant positive correlation at the level of α=0.01. The average value of various form nitrogen in the ditch sediment was the highest in the laternal canal near the fish pond, while the highest phosphorus content was observed in the branch canal near the residential area.

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Analysis of agricultural climate resources change during mid-season rice growth period in Suizhou city

YANG Tao, FENG Wen-li, BAO Feng-xia, ZHANG Zhi-sheng, CHENG Jian-ping

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 82-87. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.017

Abstract （414）

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Based on the daily ground surface meteorological observation data of two national meteorological stations in Suizhou city from 1959 to 2018, the variation characteristics of agricultural climate resources such as sunshine, temperature and precipitation in this region under the background of climate change were calculated and analyzed. The results showed that the climatic tendency rate of accumulated temperature ≥10 ℃ in Suizhou was 30.6 (℃·d)/10 years, with a significant increasing trend. The trend rate of accumulated temperature ≥10 ℃ in Guangshui city was 82.5 (℃·d)/10 years. There was no significant increase trend of accumulated temperature ≥20 ℃ in Suizhou city; The cumulative temperature ≥20 ℃ had a significant increasing trend, and the climatic tendency rate ≥20 ℃ was 89.2 (℃·d)/10 years. The climate tendency rate of sunshine hours in Suizhou city and Guangshui city were -35.4 h/10 years and -25.2 h/10 years, respectively, and both showed a significant decreasing trend. The trend of solar radiation reduction in Suizhou city was significant, and the trend rate of solar radiation climate was -42.4 MJ/(m²·10 years), while the trend of solar radiation reduction in Guangshui city was not significant. The annual precipitation fluctuated greatly in the growing season and the variation trend was not significant. The climate tendency rate of reference evapotranspiration in Suizhou city was -24.7 mm/10 years, and the variation trend of reference evapotranspiration in Guangshui city was not significant.

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Effects of planting years and cultivation methods on heavy metal accumulation in vegetable soil

WANG Su-ping, DU Lei, GUO Cui-ying, HUANG Xiang, ZHANG Gui-you, HONG Juan, LIAN Zhi-cheng, JIANG Li, ZHANG Li-hong, YE Li-xia, CHEN Gang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 88-91. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.018

Abstract （464）

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In order to study the effects of different planting years and cultivation methods on the accumulation of heavy metals and their bioavailability, the contents and bioavailability coefficients of 8 heavy metals (As, Cd, Cr, Cu, Ni, Pb, Zn, Hg) in vegetable soil with planting years less than 5, 5~10, 10~15 years and 15~20 years were studied. At the same time the content of heavy metals and bioavailability coefficient of soil under open and protected cultivation were analyzed. The results showed that the content of As, Cd, Ni and Pb increased with the increase of planting years, the effective state content and bioavailability of Cd, Cr, Cu, Pb and Zn were the highest and biggest in 10~15 years, the content of As, Cd, Hg and Ni was higher culture in open field than in the greenhouse soil, the content of Cr, Cu, Pb and Zn was higher culture in greenhouse soil than in open field. The bioavailability coefficient of Ni elements culture in the open field was higher than that in the greenhouse soil, and that of As, Cr, Cd, Cu, Hg, Pb, Zn elements culture in the greenhouse soil was higher than that in the open field. The average contents of As, Cr, Cu, Pb and Zn in the soil planted with leafy vegetables were higher than those with fruits, fruits and rhizomes. The average content of Hg was the highest in the soil planted with melons and fruits, and the average contents of Cd and Ni were the highest in the soil planted with rhizome vegetables.

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Analysis and ecological risk assessment on heavy metal contents of farmland soils in Wuhan East Lake High-Tech Development Zone

ZHANG Gui-you, DU Lei, WANG Su-ping, HUANG Xiang, JIANG Li, HONG Juan, LIAN Zhi-cheng, ZHANG Li-hong, YE Li-xia,CHEN Gang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 92-95. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.019

Abstract （464）

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A total of 58 soil samples were collected from farmland soils in Wuhan East Lake High-Tech Development Zone. The contents of 8 heavy metals (Pb, Cd, Cr, Hg, As, Ni, Cu, Zn) were analyzed. The single factor pollution index and Nemerow synthetical pollution index were used to evaluate the heavy metals in farmland soils in this area based on the soil environmental quality risk management and control standard. The results showed that the farmland soil in Wuhan East Lake High-Tech Development Zone was in a clean level, and there was slight Cd pollution in a small area (1.72%). There was no Pb, Ni, Cr, Cu, Hg, Pb pollution were found.

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A field efficacy of jinggangmycin and thifluzamide on corn sheath blight

YUAN Wei-jun, ZHOU Zhong-xiong, ZHOU Zhong-quan, CHENG Ting-ting, LIU Mei-ling, XIANG Li-bo

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 96-98. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.020

Abstract （973）

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To evaluate the control effects and the effects on yield of jinggangmycin and thiofuramide on maize sheath blight, the field trials were carried out by spraying in early stage of maize sheath blight. The results showed that 24% of jinggangmycin AS 600, 1 200 mL/hm² and 240 g/L thifluzamide SC 150, 300, and 600 mL/hm²all had better control efficacy on corn sheath blight. The control effects was 77.90% to 88.53%, and 1 000-grain weight was significantly higher than that of the control.

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Identification of root rot diseases from Hippeastrum rutilum

MENG Qing-zhong, LI Xiao-jian, YI Xian-da, LI Guo-rong, ZHANG Tao, LYU Bo

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 99-100. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.021

Abstract （470）

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The pathogen from the root tissue of Hippeastrum rutilum was isolated by tissue isolation method, and the pathogen was identified by morphological observation and ITS sequence analysis. The results showed that the colony of the pathogen was round, the hyphae were white villous at first, and gradually changed to light yellow with time, and the conidia of the pathogen strain had two forms, the large conidia were straight or slightly curved into sickle shape, there were more transverse septum, and the small conidia were oval or oval with 1~2 transverse septum. The pathogen was identified as Fusarium solani by morphology, pathogenicity determination and ITS sequence analysis.

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The identification and screening of fungicides against Fusarium culmorum from stem of walnut

WANG Peng-cheng, FANG Ai-ping, QU Ding-bin, PENG Xin, JIANG Li, JIN Tai-xing, SUN Chang-cheng, YANG Zheng-rong, JIA Qie

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 101-105. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.022

Abstract （640）

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In recent years, the black rot on young walnut stems has seriously affected the sound development of the industry in this area. In order to clarify the pathogen and improve the effect of managing this disease, identification and screening of the fungicide against the pathogen from walnut young stem were carried out in laboratory. The results showed that the black rot on walnut was mainly caused by Fusarium culmorum and the pathogen was sensitive to different fungicides. Among them, the toxicity of 40% flusilazole emulsifiable concentrate was the strongest one with an EC₅₀ of 0.383 7 μg/mL, followed by 43% tebuconazole suspension with an EC₅₀ of 9.192 1 μg/mL, and 70% thiophanate methyl wettable powder with an EC₅₀ of 2 010.961 μg/mL. In the practice of pathogen control, 40% flusilazole emulsifiable concentrate and 43% tebuconazole suspension should be considered as the main control agents Fusarium culmorum from walnut stem.

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Study on the screening method of microbial herbicide activity

ZHANG Zhi-gang, WANG Kai-mei, WU Zhao-yuan, WAN Zhong-yi, FANG Wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 106-109. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.023

Abstract （344）

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Using [Cynodon dactylon (L.) Pers.] and (Amaranthus retroflexus L.) as materials three different screening methods were used to screen the activity of microbial herbicides in laboratory. The results showed that the microplate method was suitable for the screening of quick acting compounds or active ingredients. It had the advantages of large screening flux, strong operability and less sample dosage. However, it could be used for the preliminary screening of compound activity due to its short culture time and poor anti pollution ability, but it was not suitable for the screening of microbial fermentation broth or living bacteria samples; The metabolism of the leaves in vitro is slower than that of the leaves in vivo, and the growth point almost stops growing, which can not fully reflect the activity of the samples to the leaves in vivo; The mini pot method is suitable for screening samples with complex components. The results are close to the advantages of field medication, strong pollution resistance and long observation time, but the dosage is relatively large, the operation is complex, and the screening flux is limited.

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Secondary metabolites from fungus NBERC_51984 with herbicidal activity

WU Zhao-yuan, ZHANG Zhi-gang, ZHANG Ya-ni, LIU Fang, FANG Wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 110-112. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.024

Abstract （420）

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In the search for pesticides of natural products from microbes, fungus NBERC_51984 with herbicidal activity was obtained. In order to clarify the active substance basis of fungus NBERC_51984, its secondary metabolites were studied. By activity tracking and large-scale fermentation, 2 compounds were isolated and identified as Pseurotin A (1) and Bisdethiobis(methylthio)gliotoxin (2) by LC-MS and NMR. Pseurotin A was found to show herbicidal activity to Amaranthus retroflexus.

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Preliminary study on degradation of acetochlor residue in soil by different types of organic fertilizer

CHANG Xiang-qian, HAN Mei-mei, LIU Dong-bi, ZHANG Shu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 113-116. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.025

Abstract （428）

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After the paddy soil in the barrel was treated with different types of organic fertilizer, the solution of acetochlor with the concentration of 1.2 g/L was sprayed, and the soil samples were taken to detect the residue of acetochlor at different days (0.5, 1, 3, 5, 7, 14, 21, 30, 45 d). The half-lifes of acetochlor after different treatments were calculated, and the feasibility of degradation of acetochlor by fertilizer treatment was analyzed. The results showed that after the application of organic water-soluble fertilizer or microbial organic fertilizer, the half-life of acetochlor in soil was significantly shorter than that of the control. After PSBGH, the half-life of acetochlor was the shortest, which was 21.77% of that of the control. After the application of AWJL, the half-life of acetochlor was the longest in all treatments, which was 61.51% of the control. It was considered that the application of organic fertilizer could be used as an effective method to degrade acetochlor residues in paddy fields.

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Identification and biological characteristics of the pathogen causing anthracnose on goldthread in Hubei province

WANG Zuo-qian, PAN Zhu-qing, ZHANG Shu, YOU Jing-mao, WANG You-ping, YANG Xiao-lin

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 117-121. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.026

Abstract （387）

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To identify anthracnose on goldthread in Hubei province, study its biological characteristics, and screen effective fungicides, there were twelve isolates isolated by tissue isolation method from infected leaves collected from Banqiao, Enshi, Hubei province. Among them, EsH2 and EsH6 succeed to infect leaves of goldthread in vitro and produce typical lesions. While morphology and ITS phylogenetic analysis indicated the two isolates belonging to Colletotrichum boninense species complex. Evaluation of culture under variants illumination conditions showing that they grew significantly slower under absence of light. And they grew more rapid on mung bean medium and PDA than obtained from others, respectively. Results of fungicide toxicity assay exhibited thiophanate-methyl, pyraclostrobin and difenoconazole were more effective fungicides than others with relatively low EC₅₀ and high correlation coefficient.

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Occurrence regularity and integrated control technology of citrus canker

WANG Zhi-jing, WU Li-ming, SONG Fang, HE Li-gang, WANG Ce, JIANG Ying-chun

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 122-123. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.027

Abstract （447）

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Citrus canker is a kind of quarantine bacterial disease, which seriously damages its leaves, branches and fruits, and then leads to the decrease of citrus yield. At the same time, it affects the commodity and sales of citrus. It has the characteristics of rapid transmission and difficult to cure. the symptom recognition and occurrence regularity of citrus canker weresummarized, the comprehensive control technology waselaborated, in order to effectively carry out prevention and control, reduce the cost of prevention and control, make the citrus production can be smoothly developed.

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Control effects of five kinds of fungicides against the wheat rhizome rot disease

WANG Hua, ZHANG Su-mei, CHANG Wei, ZHANG Xue-jiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 124-127. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.028

Abstract （1807）

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The study aims to find out high effective fungicides which had better control on wheat rhizome rot disease in filed in Honghu city, Hubei Province. Two methods were used： seed dressing and root spraying. The results showed that the control effects of five kinds of fungicides (50% carbendazim, 43% tebuconazole, 19% propiconazole, 21% jinggangmycin,50% formosa) in the seed dressing test were 25.3%~56.9%. Among them, 43% tebuconazole (1500 ml/hm²) showed the highest control effect (56.9%). At the same, the control effect of 19% propiconazole (900 g/hm²) and 21% jinggangmycin (750 mL/hm²) was also higher (53.1% and 50.0%, respectively). The control effects of five kinds of fungicides (60% carbendazim, horizon (430 g/L), 19% propiconazole, 24% jinggangmycin,weifu (400 g/L)) in the root spraying test were 11.8%~54.4%. Among them, the horizon (430 g/L) (180 g/hm²) showed the highest control effect (54.4%). The control effects of 19% propiconazole (900 mL/hm²) and horizon (430 g/L) (135 g/hm²) were also higher (49.3% and 43.2%, respectively). To control the wheat rhizome rot in Honghu city, the horizon (430 g/L) (180 g/hm²) and 19% propiconazole (900 mL/hm²) for seed dressing should be selected; 43% tebuconazole (180 g/hm²) and 19% propiconazole (900 mL/hm²) for the root spraying should be selected.

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Growth regularity of precious wood species Phoebe zhennan in Chongqing city

XUE Pei-pei, CHEN Ben-wen, ZHU Yuan-chun, QIAN Tao

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 128-132. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.029

Abstract （737）

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Based on the material of stem analysis, Phoebe zhennan natural secondary forest of state forest farm in Yongchuan district of Chongqing city was analyzed. The results showed that,the mean DBH of 60-year Phoebe zhennan was 38.81 cm, mean annual DBH increment was between 0.63 cm and 0.81 cm. The mean height of 60-year Phoebe zhennan was 35.30 m, mean annual height increment was 0.68 m. The maximum of current annual DBH increment and height increment were 1.00 cm and 1.00 m, and they were occurred from 15 years to 20 years, the minimum of current annual DBH increment and height increment were occured in 55~60 years, they were 0.31 cm and 0.14 m, respectively. Until the 60th year, the growth of volume is in a state of continuous growth. The volume of 60-year Phoebe zhennan was reached 1.713 1 m³, mean annual volume increment was 0.017 4 m³. The maximum of current annual volume increment occurred at 45-year-old and it was 0.047 5 m³. The growth dynamic models of height,DBH and volume of Phoebe zhennan were established with the cubic polynomial, the goodness of fit all exceed 99%. Three regression equations were y=-5.275+1.203x-0.007x²-0.000 006x³,y=-5.109+1.087x-0.004x²-0.000 06x³,y=0.182-0.035x+0.002x²-0.000 002x³, where y is DBH, height and volume, x is age. Comparing with fast growing tree species Cunninghamia lanceolata, the height and DBH increment of 38-year Cunninghamia lanceolata were 18.34 m and 25.52 cm, the tree height and DBH growth rate of Phoebe zhennan were higher than that of Cunninghamia lanceolata, in addition to the high velue of Phoebe zhennan wood, it can be widely cultivated and applied as the precious tree species in China.

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Study on the dihydromyricetin in Ampelopsis grossedentata

ZHENG Lin, GAO Shi-wei, LIU Pan-pan, ZHENG Peng-cheng, GONG Zi-ming

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 133-134. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.030

Abstract （511）

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The content of dihydromyricetin(DMY)in Ampelopsis grossedentata from different areas was comparatively analyzed,the results showed that the content of DMY was ranked Lijiang city in Yunnan province>Jinxiu Yao Autonomous county of Guangxi Zhuang Autonomous region> Wuping countyin Fujian>Zhangjiajie city in Hunan province>Laifeng county of Enshi Tujia and Miao Autonomous Prefecture from high to low. The content of DMY in Ampelopsis grossedentata from Lijiang city in Yunnan province was significantly highest, and the content of DMY from Laifeng county of Enshi Tujia and Miao Autonomous Prefecture was significantly lowest,there was no significant difference among other areas. On the basis, DMY was extracted from Ampelopsis grossedentata by ultrasonic, microwave, wall breaking methods with ethanol,the results showed that microwave-assisted ethanol extraction could achieve green, energy-saving and high-efficiency extraction state with less amount of ethanol, shorter extraction time and lower solid-liquid ratio.

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Investigation and preliminary analysis on nutritional factors of soil, leaf and fruit in citrus orchards in Hubei province

HE Li-gang, WU Li-ming, JIANG Ying-chun, ZHANG Lian-lian, JIANG Yan, YAO Jing-lei, WANG Zhi-jing, SONG Fang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 135-140. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.031

Abstract （457）

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The nutrition factors of soil, leaf and fruit, and quality of fruits in citrus orchards from Yidu, Dangyang, and Zigui county in Hubei province were investigated and analyzed, referred to the standard of diagnostic on citrus nutrition, the results demonstrated that boron (B) was deficient in soil of Yidu and Dangyang, where manganese (Mn) was excessive while zinc (Zn) was deficient, calcium (Ca) was excessive in soil of Zigui; the soil from three different areas contained insufficient nitrogen (N) and Zn, but too much Mn; due to the main cultivars in above areas, the nutritional levels were different from each other. Based on the above conclusion, the soil environment and plant nutritional levels were determined and theoretically contributed to the nutritional diagnostic, fertilizing efficiency and ecological improvement for the development of the citrus industry in Hubei province.

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Polymorphism of porcine OLR1 gene and its association with liter size trait of Xidu black pigs

QIAO Mu, LI Kang-chi, WU Jun-jing, WU Hua-yu, ZHOU Jia-wei, MEI Shu-qi, PENG Xian-wen

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 141-143. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.032

Abstract （440）

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In order to explore the effect of OLR1 gene genetic polymorphism on litter size of Xidu black pigs, the direct sequencing method was used to detect the genetic polymorphism of OLR1 gene in the Xidu black pigs, and the association analysis with litter size was carried out. The results showed that there was a T/C mutation at 7 bp of intron 4, and there were three genotypes TT, TC and CC; The total number born and number born alive of individuals with TT genotype were extremely significantly higher than those with TC and CC genotype (P<0.01), indicating that the mutation had a significant impact on litter size traits of Xidu black pig.

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Identification of HDLBP SNPs and its association with egg performance traits

HUANG Tao, CHENG Lu, MO Chang-huan, WANG Xiao-zhong, SHEN Jie, PI Jin-song, GONG Yan-zhang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 144-147. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.033

Abstract （384）

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6 SNPs in the full length of HDLBP transcript were identified as a synonymous mutation in adult hens. Association between two SNPs and egg performance traits were analyzed in Xinhua E-strain chickens, which showed that they were highly significantly associated with AFE and EW (P<0.05), suggesting that it was a candidate gene related with egg production. The HDLBP mRNA in different sized follicles showed that the expression was progressively increased in follicles from undifferentiated follicles to SYFs, and maintain stable in pre-ovulatory follicle. Besides, FSH, EGF, VEGF with dosage dependent could regulate the expression of HDLBP in granulosa cells of phases, while Follistatin showed no effect.

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Study on the extraction technology of essential oil from Damascus rose

YANG Qing, LIU Xing-le, LI Li-yun, WANG Dun, WANG Xue, ZHANG Shu-juan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 148-150. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.034

Abstract （453）

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The extraction technology of essential oil from Damascus rose was studied by steam distillation. The effects of dosage, extraction time, ratio of material to liquid, and reflux time on the extraction rate of essential oil were investigated. The results showed that, it will be best when the dosage was 200 kg, the extraction time was 4 hours, the ratio of material to liquid was 1∶4, and the reflux time was 2 hours,which can come up to 3.19?.

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Development and nutritional value evaluation of the potato flour ham

CAI Sha, MEI Xin, HE Jian-jun, SUI Yong, SHI Jian-bin, XIONG Tian, CHEN Xue-ling, FAN Chuan-hui, CAI Fang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 151-155. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.035

Abstract （519）

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Taking potato flour as raw materials, using sensory evaluation and texture properties as the indicators,through single factor experiments and orthogonal design experiments,the factors affecting the texture properties and sensory evaluation of potato flour ham were optimized,such as lean meat percentage, mashed potato percentage, soy isolate protein percentage, water percentage. The results showed that, when the lean meat percentage was 96%, mashed potato percentage was 10%, soy isolate protein percentage was 6%, water percentage was 40%, the sensory evaluation of potato flour ham was the highest, which was 87.4. Compared with the commercial hams, potato flour ham had higher protein content and lower fat content, which was more suitable for all kinds of people.

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Study on antioxidant activity and HPLC fingerprints from different polarity parts of Oxalis corniculata L.

LI Xiao-na, JI Yong-hui, WANG Jing-long, ZHANG Li-hua, ZANG Yuan-long, YANG Bin, LIU Chun-he, LIU Chun-jiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 156-163. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.036

Abstract （396）

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The contents of polyphenol, flavonoids, polysaccharide in the bulb of Oxalis corniculata L. were measured by ferrous tartrate method, erinitrit-aluminum nitrate-sodium hydroxide method, phenol-sulfuric acid method and DNS method. Using acetonitrile and 0.1% phosphoric acid as mobile phase and gradient elution, the fingerprints of different polar parts of alcohol extract of Oxalis ferruginosa were investigated. The antioxidant activity in vitro was determined by 1,1-diphenyl-2-picrylhydrazine radical scavenging method and hydroxyl. OH radical scavenging method with ascorbic acid as positive control. The polyphenol contents of the two parts were 5.45 and 4.73 mg/g. The flavonoids contents of the two parts were 16.62 and 7.70 mg/g. The polysaccharide contents of the two parts were 17.00 and 121.02 mg/g. 32 peaks were calibrated on the basis of peak 21, and 31 inner cores were calibrated were set at 280 nm. The common peaks in different polar parts are the outer skin： petroleum ether phase 8, chloroform phase 22, ethyl acetate phase 22, n-butanol phase 26, water phase 9; inner core： petroleum ether phase 14, chloroform phase 21, ethyl acetate phase 17, n-butanol phase 19, water phase 10. In the determination system of antioxidant activity, the outer skin and inner core of bulb had certain antioxidant activity in different polar parts. The chloroform phase and ethyl acetate showed better antioxidant activity than the two free radicals. The contents of polyphenol, flavonoids and polysaccharide were rich in the bulb of Oxalis corniculata L.. After extraction by petroleum ether, chloroform, ethyl acetate and n-butanol, the chemical fingerprints and antioxidant ability of the ethanol extracts were different, which could provide reference for the discovery of effective substances in the later period.

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Study on rice-Cadmium of safety production threshold of cultivated land in high geological background area of Hubei province

YI Tian, PENG Li-jun, CUI Wen-wen, WANG Ming-rui, YAO Jing-jing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 164-168. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.037

Abstract （368）

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It is inconsistent to exceed the Cd standard in the high geological background area and the Cd standard in the rice area, so the safe production threshold of rice-cadmium cultivated land suitable for the high geological background area should be selected.This study based on the soil heavy metals-rice, physical and chemical means of correlation analysis, through spatial interpolation and parameter calculation, get the following results,Cd content in soil and soil physical and chemical indicators (pH, organic matter, cation exchange capacity) is weak, the linear relationship between the regions with high explain geological background due to factors such as soil background value, the soil pollution is serious.There is a close relationship between Cd in rice and soil physicochemical properties, which may be because although the background value of heavy metals in high geological background is high, it is not affected by human activities and has little impact on Cd content of rice planted.By calculating the required parameters and interpolation, the cultivated land safety threshold of the geological high background area was preliminarily judged to be 0.31 mg/kg. The reasonableness of the predicted results was tested, and the result showed that the cultivated land security threshold in the geological high background area was set reasonably.

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Physiological response and transcriptome analysis of cotton leaf in the two-leaf period under low temperature

WANG Xiao-man, WANG Qiong-shan, WANG Xiao-gang, ZHANG Jiao-hai, ZHANG You-chang, ZHANG Xing-zhong, BIE Shu, XIA Song-bo

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 169-176. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.038

Abstract （394）

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CN01 and SJB016 (a low temperature resistant cotton) were used as materials to study the effects of low temperature treatment on cotton in the two-leaf period. Firstly, We separately measured the contents of malondialdehyde (MDA), soluble sugar (SS) and proline (Pro) in CN01 and SJB016 at 25 ℃ (CK) and 12 ℃ (low temperature treatment) for 0, 3, 6, 12, 24, 48 and 72 h. It was found that the contents of MDA, SS, and Pro in CN01 and SJB016 increased after 12 ℃ treatment compared with 25 ℃. After low temperature treatment, the content of MDA in SJB016 increased less than CN01, the content of SS in SJB016 increased larger than CN01, and the content of Pro in SJB016 increased less than CN01. Transcriptome analysis of cotton leaves in two-leaf period after low temperature treatment for 6 hours showed that the photosynthesis-related genes were up-regulated in CN01 after 12 ℃ treatment, while the genes of photosystem II oxygen-releasing complexes (PsdP, PsdQ, et al) were down-regulated in SJB016, compared with 25 ℃. The up-regulated differentially expressed genes associated with phytohormone were more than down-regulated genes in CN01 and SJB016, and ethylene-responsive transcription factors ERF053, ERF020, and ERF061 significantly up-regulated. These genes are deemed to play an important role in improving the cold resistance of cotton.

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Isolation and identification of swine Mannheimia haemolytica

LIU Ze-wen, YUAN Fang-yan, LIU Wei, GAO Ting, ZHOU Dan-na, YANG Ke-li, DUAN Zheng-ying, GUO Rui, LIANG Wan, TIAN Yong-xiang

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 177-178. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.039

Abstract （478）

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A gram negative bacilli was isolated from fattening pigs of a pig farm with pneumonia in Hubei province. After pathogen isolation, gram staining, PCR identification and biochemical identification, it was identified as Mannheimia haemolytica. The bacterium was gram-negative short bacilli with two poles stained. The results of sequence comparison showed that the homology rate of the gene sequence of the strain was more than 99.7%, and its biochemical characteristics accorded with the biochemical characteristics of the strain. The strain 2×10⁸ CFU/mL can kill mice and has strong pathogenicity. The results of drug sensitivity test showed that the strain was sensitive to ampicillin, cefotaxime and ciprofloxacin.

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Research on the identification of entrepreneurial opportunities of the bottom groups in the process of urbanization

HAN Lu, BAO Hai-jun, CHEN Meng-yuan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 179-183. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.040

Abstract （373）

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Taking eight bottom groups in the urbanization process of Qingyang city of Gansu province as the typical case, using case study method, this study constructed the operation model of the identification of the entrepreneurial opportunities of the bottom groups based on the theoretical framework of the entrepreneurial opportunities identification, and analyzed the factors affecting the entrepreneurial opportunities identification of the bottom groups. The results showed that ,the identification of entrepreneurial opportunities of the bottom groups in the process of urbanization was mainly influenced by two factors： Individual characteristics and entrepreneurial environment, and the influencing factors in different stages were different. Finally, the corresponding countermeasures were put forward to improve the ability of the bottom groups to identify the entrepreneurial opportunities.

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Marketing strategy optimization of geographical indication agricultural products enterprises under the background of industry poverty alleviation：Take Chuju in Chuzhou city as an example

SHI Lei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 184-188. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.041

Abstract （470）

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Geographical indication agricultural products had distinct regional characteristics and competitive advantages, which was an effective way to realize rural industrial poverty alleviation and rural revitalization. This study selected Chuju, an agricultural product with geographical indication, from the perspective of product marketing, analyzed the problems existing in product marketing of Chuju enterprises under the background of industrial poverty alleviation, and put forward the optimization suggestions of marketing strategies, so as to help the regional industrial poverty alleviation and rural revitalization development.

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Research on optimization of agricultural products cold chain logistics distribution path based on customer satisfaction

WEI Qing-hao, WU Xian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 189-194. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.042

Abstract （469）

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The lack of cooperation between the traditional agricultural products cold chain logistics distribution stations in China not only caused waste of resources, but also lead to high time cost and low customer satisfaction. Based on the optimization of agricultural products cold chain logistics distribution path, this study established the collaborative distribution optimization model from the perspective of customer satisfaction, and verified the effectiveness of the model through case analysis. The results showed that, collaborative distribution can effectively reduce the distribution cost and improve the distribution efficiency, which provided the corresponding guidance for cold chain logistics enterprises to choose high-quality distribution path.

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Impact of COVID-19 on Wuhan residents' willingness of rural homestead land transfer

LIU Zhi-ling, WANG Zhen-wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 195-198. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.043

Abstract （439）

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Stduy on residents' willingness of rural homestead land transfer can provide intellectual support to promote the pilot reform of the separation of “Theree Rights” in rural homestead land and furtherly to help realize rural revitalization strategy. This study employed regulatory focus theory to analyze the influence mechanism of COVID-19 on Wuhan residents' willingness of rural homestead land transfer. It compared Wuhan residents' willingness of rural homestead land transfer from Janauray 23rd to March 10th in 2020 with that in 2019,then the lag correlation analysis was conducted. The results showed that, Wuhan residents' willingness of rural homestead land transfer increased by 39.52 percent because of COVID-19. It had high positive correlation with the number of new confirmed cases, new deaths and cumulative deaths. The response of Wuhan residents' willingness of rural homestead land transfer to them lagged 13 or 14 days. Some suggestions were given to motivate Wuhan residents to transfer rural homestead land.

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Analysis on the characteristics of external cooperation of Xinjiang regional innovation system elements

WU Zheng-ping, SHEN Gao-yan, LI Xiu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 199-205. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.044

Abstract （385）

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The elements of regional innovation system in Xinjiang included universities, enterprises and scientific research institutions.Based on the data from 2013 to 2017, this study analyzed the characteristics of external cooperation of Xinjiang regional innovation system elements by analyzing the characteristics of core papers published by Xinjiang undergraduate colleges with external cooperation ,the characteristics of patents invented by Xinjiang main board listed companies with external cooperation, and the characteristics of Xinjiang technology flow region. The results showed that,① the regional distribution of core papers published by Xinjiang undergraduate colleges with external cooperation was relatively wide, and mainly in the eastern economically developed areas;② the number of patents invented by Xinjiang main board listed companies with external cooperation was less, and the technical cooperation between enterprises and external cooperation was less; ③ the distribution of Xinjiang technology flow region was mainly concentrated in the central and eastern areas. Finally, some countermeasures and suggestions were put forward to improve the technical cooperation between Xinjiang and external cooperation.

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Research on the legal obstacles and countermeasures of using collective construction land to build rental houses in Guangzhou city

ZHOU Wei-ji, CHENG Xiao-man, WEN Han-feng, YE Jun-feng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 206-209. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.045

Abstract （319）

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From the perspective of law and management, this study made a systematic analysis of the legal obstacles of using collective construction land to build rental houses in Guangzhou city through the methods of literature research and comprehensive analysis. The results showed that, there were some problems in the using collective construction land to build rental houses in Guangzhou city, such as the conflict with current laws and regulations, lack of legal basis, involving many stakeholders, fuzzy income distribution system and blocked development and financing channels. Therefore, it was suggested that the current laws and regulations should be amended timely, the property right system should be improved, the supervision mechanism should be established and perfected, the financing market should be stabilized, and the financial policy should be relaxed.

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Development potential of agricultural products trade between China and ASEAN under the background of “The Belt and Road”： Based on trade gravity model

WANG Rui, YU Xin-xin, LIU Yu

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 210-214. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.046

Abstract （523）

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Based on the trade data of agricultural products between China and ASEAN from 2008 to 2017, this study analyzed the competitiveness and complementarity of bilateral trade of agricultural products between China and ASEAN by using the regional revealed comparative advantage index (RRCA) and trade complementarity index (TCI), and calculated the trade potential of agricultural products between China and ASEAN in 2013 and 2017 through the trade gravity model. On this basis,the development potential of agricultural products trade between China and ASEAN under the background of “The Belt and Road” was analyzed,and the countermeasures and suggestions to promote the development of bilateral trade of agricultural products between China and ASEAN were put forward.

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Analysis on the green production path of tea farmers in southern Shaanxi guided by tea geographical indications

CHENG Dang-wei

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 215-218. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.047

Abstract （342）

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How to promote agricultural supply-side structural reform as the main line, change the mode of agricultural development, promote the transformation of farmers' production mode from conventional production to green production, and improve the quality and efficiency of agricultural products was an urgent task for the sustainable development of tea industry in southern Shaanxi. Based on the management of tea geographical indications in southern Shaanxi, this study explored the way to guide tea farmers' green production with tea geographical indications, improved the quality and production efficiency of tea geographical indications, promoted rural revitalization and sustainable development of tea industry, increased efficiency of tea industry and the income of tea farmers.

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Evaluation measure and analysis of agricultural science and technology popularization efficiency in Hubei province：Based on the survey of peasant household data in Hubei province

ZHOU Yan, GUO Hong-xi, KE Yan-ruo, WU Cheng-cheng, XIA Juan, WANG Zhi-hong

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 219-225. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.048

Abstract （362）

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Agricultural science and technology extension service was an important means to improve agricultural production efficiency in China, which was the key to the green and efficient development of agriculture. In this paper, the data were obtained through the peasant survey in the sample area of Hubei Province, and the Ordinal Logistics model was used for econometric analysis to measure the evaluation of peasant households on the economic efficiency, social efficiency and ecological efficiency of agricultural science and technology extension service. First, the results showed that the field demonstration and household guidance were the most popular methods for farmers.Second, the ranking of farmers' approval attitude towards various benefits of agricultural science and technology extension service was： economic benefits > ecological benefits > social benefits. Third, when the other conditions remain unchanged, the two popularization methods of “observation and learning” and “exchange meeting” can significantly improve farmers' evaluation of the service effect, while “issuing technical guidance materials” was not conducive to the improvement of farmers' evaluation of the service effect of agricultural science and technology popularization. Finally, it was found that the government should improve the cultural and educational level of farmers in various aspects, strengthen the exemplary leading role of agricultural science and technology demonstration households, and increase policy support and fund input to improve the efficiency of agricultural science and technology popularization services.

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Strategies for high-quality development of rural tourism in Henan province under the strategy of rural revitalization

WEI Yan, LI Bao-guo, YUAN Meng

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 226-229. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.049

Abstract （451）

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Rural tourism is an important part of rural revitalization, and the dialectical development relationship between them is mutual support and mutual promotion. This study analyzed the requirements for the high-quality development of rural tourism under the strategy of rural revitalization, expound the current situation and existing problems of rural tourism development in Henan province, and put forward the development strategies of scientific planning, innovation of rural tourism products, creation of rural tourism brands, improvement of supporting facilities, effective marketing, protection of rural ecology, and assistance of tourism poverty alleviation.

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Difficulties and enlightenment of the development of farmers' cooperatives in Xiaogan city

WU Ya-ling, HUANG Wen-wen, LIU Hai-bo, LEI Shu-yan

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 230-232. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.050

Abstract （329）

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In recent years, Xiaogan city has strengthened the policy support, promoted the farmers' cooperatives to become bigger and stronger, promoted the standardized development of farmers' cooperatives, improved the service driving ability of farmers' cooperatives, and significantly improved the development quality of farmers' cooperatives. On the basis of summarizing the main measures for the development of farmers' cooperatives in Xiaogan city, in view of the main problems faced by the development of farmers' cooperatives in Xiaogan city, this study put forward some countermeasures and suggestions from the aspects of characteristic industry support, financial insurance docking, agricultural talent training, supporting facilities construction and so on.

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Exploration on the strategy of rural revitalization in Shaanxi based on the sustainable development of rural society

LI Jing

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 233-235. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.051

Abstract （371）

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Excellent local culture was the inherent wealth of rural society and the foundation and power of sustainable development of rural society. This study expounded the foundation of Shaanxi rural revitalization development, analyzed the examples of Shaanxi excellent local culture helping rural revitalization development, discussed the opportunities and challenges faced by Shaanxi excellent local culture inheritance, and put forward suggestions on Shaanxi excellent local culture helping rural revitalization development, so as to drive the development of rural industry, and lay the cornerstone of effective governance of rural society.

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Study on the evolution of symbiotic behavior pattern of multi-agents in tourism community governance：Taking Huzhu Tu Nationality Homeland Park in Qinghai province as an example

SHAO Li, TANG Zhong-xia, XIANG Cheng, REN Xi-xian, CHAI Jian

HUBEI AGRICULTURAL SCIENCES 2020, 59 (24): 236-241. DOI: 10.14088/j.cnki.issn0439-8114.2020.24.052

Abstract （405）

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Based on symbiosis theory, this study took Huzhu Tu Nationality Homeland Park in Qinghai province as the case, selected government, enterprises, residents and tourists as the symbiotic main units, analyzed and discussed the symbiotic behavior pattern and evolution of multi-agents in tourism community governance through two field survey data. The results showed that,①there was a positive symbiotic relationship between different governance agents in 2012 and 2017; ② there were differences in the interaction and influence between different governance agents in the two periods; ③compared with 2012, the symbiotic correlation among government-enterprises, government-tourists, residents-enterprises and residents- tourists in 2017 was strengthened, the symbiotic correlation between government-residents and enterprises-tourists in 2017 was weaken; ④the symbiotic behavior pattern has evolved from the R-G-E-T pattern in 2012 to the E-R-G-T pattern in 2017.

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